Stem cell culture device and method

Abstract

The invention discloses a stem cell culture device and method, and belongs to the field of stem cell culture. A stem cell culture device comprises a culture box and a germicidal lamp fixed to the top of an inner cavity of the culture box, the middle of the inner cavity of the culture box is rotationally connected with a rotating disc, the bottom of the rotating disc is fixedly connected with a U-shaped piece, the other end of the U-shaped piece is fixedly connected with a culture disc, the culture disc is attached to the inner wall of the culture box in a sliding mode, and a culture dish is installed at the top of the culture disc. An adsorption assembly for fixing a culture dish is arranged at the bottom of the culture tray; according to the device, a spraying pipe drives a spraying disc to rotate, so that a nutrient solution is sprayed out in a rotating manner along atomizing nozzles at all positions, and a culture disc can drive culture dishes at all positions to rotate in cooperation with the fitting friction effect between a friction wheel and a friction ring, so that the comprehensive and uniform spraying effect is achieved; the nutrient solution adding efficiency is improved, meanwhile, it is guaranteed that the quality of the nutrient solutions added into the culture dishes is the same, and therefore the culture effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, and in particular to a stem cell culture device and method. Background Art

[0002] Stem cells are a type of undifferentiated cells with the ability to self-renew and multidirectional differentiation potential. They can divide indefinitely in vivo or in vitro without losing their differentiation ability, and can also differentiate into various types of mature cells. At present, in order to better understand the basic principles of life sciences and find new methods to treat diseases, researchers have conducted a series of scientific studies on stem cells. In order to provide enough stem cells for scientific research, stem cells usually need to be cultured to meet scientific research needs.

[0003] At present, in the process of stem cell culture, in order to maintain the various nutrients necessary for the survival and proliferation of stem cells, it is necessary to regularly add nutrient solution to the culture dish. Stem cell culture is usually carried out in multiple groups at the same time. At this time, adding nutrient solution one by one is inefficient, and the dosage of each addition needs to be controlled, which is cumbersome. Moreover, after the nutrient solution is added to the culture dish, it cannot achieve uniform contact with the stem cells, resulting in different growth and reproduction efficiencies of stem cells in various parts of the culture dish, affecting the culture effect. Therefore, a stem cell culture device and method are proposed. Summary of the invention

[0004] The purpose of the present invention is to solve the problems in the prior art that when multiple groups of stem cells are cultured simultaneously, the efficiency of dripping nutrient solution is low; and the nutrient solution and stem cells cannot quickly achieve uniform contact, affecting the culture effect, and to propose a stem cell culture device and method.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A stem cell culture device comprises an incubator and a sterilizing lamp fixed on the top of its inner cavity, wherein a rotating disk is rotatably connected to the middle of the inner cavity of the incubator, a U-shaped piece is fixedly connected to the bottom, a culture tray is fixedly connected to the other end of the U-shaped piece, the culture tray slides in contact with the inner wall of the incubator, a culture dish is installed on the top of the culture tray, and an adsorption component for fixing the culture dish is arranged at the bottom of the culture tray, and also includes: a spray component, which is arranged on the incubator and is used to transport nutrient solution into the culture dish.

[0007] In order to improve the efficiency of adding nutrient solution, preferably, the spray assembly includes a spray pipe, which is rotatably connected to the top of the inner cavity of the incubator, a spray plate is fixedly connected to the outer wall of the spray pipe, and the spray plate is communicated with the inner cavity of the spray pipe, a plurality of groups of atomizing nozzles are fixed at equal intervals on the lower part of the outer wall of the spray plate, and the atomizing nozzles are communicated with the inner cavity of the spray plate, a liquid storage tank is fixedly connected to the top of the incubator, the top end of the spray pipe extends through and extends above the top of the liquid storage tank, and the spray pipe is communicated with the inner cavity of the liquid storage tank, and a centrifugal part for driving the spray pipe to rotate is provided on the top of the incubator.

[0008] Furthermore, the centrifugal part includes a transmission box, which is fixedly connected to the top of the liquid storage tank. A driving shaft is rotatably connected to the transmission box. The top of the spray pipe passes through the transmission box, and the driving shaft and one end of the spray pipe that is close to each other are meshed and connected via a bevel gear set. A driving motor is fixedly connected to the top of the incubator, and the output shaft of the driving motor and the driving shaft are transmission-connected via a pulley set.

[0009] In order to improve the uniformity of adding nutrient solution, preferably, three groups of U-shaped parts are arranged at equal intervals, friction rings are fixedly connected between the three groups of U-shaped parts, a linkage shaft is fixedly connected to the bottom of the spray plate, the bottom end of the linkage shaft passes through the annular cavity formed by the culture plate and the culture dish and extends to the friction ring, the bottom end of the linkage shaft is fixedly connected to a friction wheel, and the friction wheel is in contact with the side wall of the friction ring.

[0010] In order to reduce the loss of nutrient solution, preferably, a liquid suction box is rotatably connected to the outer wall of the driving shaft, a return pipe is fixedly connected to the lower part of the side wall of the liquid suction box, the other end of the return pipe is fixedly connected to the upper part of the outer wall of the liquid storage tank, and the two ends of the return pipe are respectively connected to the liquid suction box and the inner cavity of the liquid storage tank, a liquid suction blade is fixedly connected to the outer wall of the driving shaft located in the liquid suction box, a guide ring is provided at the bottom of the inner cavity of the incubator, a liquid collecting bin is provided at the bottom of the incubator, the liquid collecting bin is connected to the inner cavity of the guide ring, a liquid extraction pipe is fixed to and connected to the upper part of the side wall of the liquid suction box, and the other end of the liquid extraction pipe is connected to the inner cavity of the liquid collecting bin.

[0011] In order to improve the culture effect, preferably, limiting grooves are evenly spaced on the top of the culture plate, a driven plate is rotatably connected in the limiting groove, the culture dish is inserted in the limiting groove and fits on the top of the driven plate, a positioning bearing is provided at the bottom of the limiting groove, the bottom end of the rotating shaft of the driven plate passes through the center of the positioning bearing and is fixedly connected to a driven wheel, and the outer wall of the driven wheel fits with the outer wall of the U-shaped part.

[0012] In order to improve the stability of the culture dish, preferably, the adsorption assembly includes a piston tube, which is fixed at the bottom of the limiting groove, the input end of the piston tube is connected to the inner cavity of the limiting groove, a piston plate is slidably connected in the piston tube, a return spring is fixedly connected between the top of the piston plate and the inner wall of the piston tube, an air guide tube is fixedly connected to the piston plate, three groups of magnetic blocks are evenly spaced at the top of the driven disk, the magnetic blocks and the piston plate repel each other magnetically, an adsorption groove is opened in the driven disk, and the bottom end of the adsorption groove is connected to the inner cavity of the limiting groove, and one-way valves are arranged at the input and output ends of the piston tube and in the air guide tube.

[0013] In order to facilitate aseptic culture, preferably, an addition tube is fixedly connected to the top of the culture box, a sealing plug is threadedly connected to the top of the addition tube, and the output end of the addition tube is located directly above the culture dish.

[0014] Furthermore, a sealing plate is plugged into the incubator, and a transparent window is provided on the sealing plate.

[0015] A stem cell culture method, comprising the following steps:

[0016] Step 1: Place the culture dish on the culture tray and sterilize the inside of the incubator;

[0017] Step 2: adding stem cells to be cultured into the culture dish;

[0018] Step 3, adding nutrient solution to the culture dish containing stem cells;

[0019] Step 4: Shake the stem cells in the culture dish to ensure that they are in even contact with the nutrient solution.

[0020] Compared with the prior art, the present invention provides a stem cell culture device and method, which have the following beneficial effects:

[0021] 1. The stem cell culture device drives the spray plate to rotate through the spray pipe, so that the nutrient solution is sprayed out from the atomizing nozzles at various locations in a rotational manner, and the friction between the friction wheel and the friction ring will cause the culture plate to rotate with the culture dishes at various locations, thereby achieving a comprehensive and uniform spraying effect, improving the efficiency of adding nutrient solution, and ensuring that the quality of the nutrient solution added to the culture dishes at various locations is the same, thereby improving the culture effect.

[0022] 2. The stem cell culture device, through the fitting and friction effect between the driven wheel and the outer wall of the U-shaped part, will cause the driven disk to suddenly obtain a rotating effect during the rotation of the culture dish. Under the action of inertia, the stem cell tissue fluid and nutrient solution in the culture dish are driven to shake, so that the stem cell tissue fluid is in uniform contact with the nutrient solution and oxygen, ensuring that each cell can obtain sufficient nutrition and oxygen supply, accelerating the cell reproduction efficiency and improving the culture effect.

[0023] 3. The stem cell culture device, through the rotation of the liquid suction blade, refluxes the nutrient solution scattered in the culture box for reuse, thereby reducing the loss of the nutrient solution. At the same time, it also continuously applies airflow thrust along the reflux pipe to the liquid storage tank, thereby increasing the falling pressure of the nutrient solution in the liquid storage tank, accelerating the speed at which the nutrient solution is sprayed out along the atomizing nozzle, improving the spraying efficiency, reducing the working time of the drive motor, reducing energy consumption, and improving energy-saving effects.

[0024] 4. The stem cell culture device, through the repulsive effect of the magnetic block and the piston plate, and the rebound effect of the reset spring, will gradually discharge the gas in the limit groove during the rotation of the culture plate, thereby generating negative pressure suction in the adsorption groove, so that the culture plate fits tightly on the surface of the driven plate, ensuring the stability of the culture plate during the shaking culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of a stem cell culture device proposed by the present invention;

[0026] Figure 2 This is a schematic diagram of the overall structure of a stem cell culture device proposed by the present invention from a side view;

[0027] Figure 3 This is a schematic diagram of the overall half-section structure of a stem cell culture device proposed by the present invention;

[0028] Figure 4 A stem cell culture device proposed by the present invention Figure 3 A schematic diagram of the partially enlarged structure;

[0029] Figure 5 A stem cell culture device proposed by the present invention Figure 3 A schematic diagram of the enlarged structure of the middle A area;

[0030] Figure 6 A stem cell culture device proposed by the present invention Figure 3 Schematic diagram of the enlarged structure of the middle B area;

[0031] Figure 7 This is a schematic diagram of the transverse cross-sectional structure of a stem cell culture device proposed by the present invention;

[0032] Figure 8 This is a schematic diagram of the bottom structure of a culture dish of a stem cell culture device proposed in the present invention.

[0033] In the figure: 1, incubator; 2, germicidal lamp; 3, rotating disk; 31, U-shaped piece; 311, friction ring; 32, incubator; 33, incubator; 4, spray pipe; 41, spray disk; 411, atomizing nozzle; 42, liquid storage tank; 43, transmission box; 431, drive shaft; 432, bevel gear set; 44, drive motor; 441, pulley set; 45, linkage shaft; 451, friction wheel; 46, liquid suction box ; 461, reflux pipe; 462, suction blade; 463, guide ring; 464, liquid collecting bin; 465, suction pipe; 5, limit groove; 51, driven disk; 511, driven wheel; 52, positioning bearing; 6, piston tube; 61, piston plate; 611, return spring; 612, air guide pipe; 62, magnetic block; 63, adsorption groove; 7, adding tube; 71, sealing plug; 8, sealing plate; 81, transparent window. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0036] Embodiment 1:

[0037] Reference Figure 1-Figure 8A stem cell culture device comprises a culture box 1 and a germicidal lamp 2 fixed on the top of the inner cavity thereof. The germicidal lamp 2 adopts the existing mature ultraviolet technology to achieve sterilization and disinfection of the inner surface of the culture box 1, ensure a sterile environment, and improve the culture effect. A rotating disk 3 is rotatably connected to the middle of the inner cavity of the culture box 1, and a U-shaped piece 31 is fixedly connected to the bottom. The other end of the U-shaped piece 31 is fixedly connected to a culture plate 32. The culture plate 32 slides in contact with the inner wall of the culture box 1. A culture dish 33 is installed on the top of the culture dish 32, and an adsorption component for fixing the culture dish 33 is provided at the bottom of the culture dish 32. The top of the culture box 1 is fixedly connected There is an addition tube 7, the top end of the addition tube 7 is threadedly connected with a sealing plug 71, and the output end of the addition tube 7 is located directly above the culture dish 33. The stem cell tissue fluid can be directly added to the culture dish 33 in the sterile environment through the addition tube 7 to achieve sterile addition operation and ensure the culture effect. A sealing plate 8 is plugged into the culture box 1, and the sealing plate 8 is used to ensure the sealing inside the culture box 1. A transparent window 81 is provided on the sealing plate 8, and the transparent window 81 is used to facilitate observation of the stem cell culture situation. It also includes: a spray component, which is arranged on the culture box 1 and is used to transport nutrient solution into the culture dish 33.

[0038] Reference Figure 1-Figure 4 and Figure 6 , wherein the spray assembly includes a spray pipe 4, which is rotatably connected to the top of the inner cavity of the incubator 1, a spray disc 41 is fixedly connected to the outer wall of the spray pipe 4, and the spray disc 41 is communicated with the inner cavity of the spray pipe 4, and a plurality of groups of atomizing nozzles 411 are fixed at equal intervals on the lower part of the outer wall of the spray disc 41, and the atomizing nozzle 411 adopts an existing pressure nozzle, and a certain pressure needs to be applied to achieve a flow-through effect, and the atomizing nozzle 411 is communicated with the inner cavity of the spray disc 41, and a liquid storage tank 42 is fixedly connected to the top of the incubator 1, and the top end of the spray pipe 4 extends through and extends to the top of the liquid storage tank 42, and the spray pipe 4 is communicated with the inner cavity of the liquid storage tank 42, and a centrifugal part for driving the spray pipe 4 to rotate is provided on the top of the incubator 1; the centrifugal part includes a transmission box 43, and the transmission box 43 is fixedly connected to the liquid storage tank 4 2, a driving shaft 431 is rotatably connected to the transmission box 43, the top of the spraying pipe 4 passes through the transmission box 43, and the driving shaft 431 and the end of the spraying pipe 4 close to each other are meshed and connected through a bevel gear set 432, a driving motor 44 is fixedly connected to the top of the incubator 1, and the output shaft of the driving motor 44 is connected to the driving shaft 431 through a pulley set 441; three groups of U-shaped parts 31 are arranged at equal intervals, and friction rings 311 are fixedly connected between the three groups of U-shaped parts 31, and a linkage shaft 45 is fixedly connected to the bottom of the spraying disc 41, and the bottom end of the linkage shaft 45 passes through the annular cavity formed by the culture disc 32 and the culture dish 33 and extends to the friction ring 311, and the bottom end of the linkage shaft 45 is fixedly connected to the friction wheel 451, and the friction wheel 451 fits the side wall of the friction ring 311

[0039] By setting the above structure, the driving motor 44 is turned on, and the transmission action of the pulley group 441, the driving shaft 431 and the bevel gear group 432 is cooperated to drive the spray pipe 4 to rotate. At this time, the spray pipe 4 will synchronously drive the spray disc 41 to rotate, and generate centrifugal force in the spray disc 41, so that the nutrient solution is sprayed out in a rotational manner along the atomizing nozzles 411 at various locations. At the same time, the friction ring 311 drives the culture disc 32 to rotate, so that the culture dishes 33 at various locations will rotate and pass under the spray disc 41, so as to achieve a comprehensive and uniform spraying effect, improve the efficiency of adding nutrient solution, and ensure that the quality of the nutrient solution added in the culture dishes 33 at various locations is the same, thereby improving the culture effect.

[0040] It should also be noted that the friction wheel 451 drives the friction ring 311 to rotate by friction force, and the friction force between the two is relatively small, that is, during operation, the rotation speed of the friction ring 311 is much lower than that of the friction wheel 451 .

[0041] Reference Figure 2-Figure 4 , wherein a liquid pipette box 46 is rotatably connected to the outer wall of the driving shaft 431, a return pipe 461 is fixedly connected to the lower part of the side wall of the liquid pipette box 46, the other end of the return pipe 461 is fixedly connected to the upper part of the outer wall of the liquid storage tank 42, and the two ends of the return pipe 461 are respectively connected to the inner cavities of the liquid pipette box 46 and the liquid storage tank 42, a liquid pipette blade 462 is fixedly connected to the outer wall of the driving shaft 431 located in the liquid pipette box 46, a guide ring 463 is provided at the bottom of the inner cavity of the incubator 1, a liquid collecting bin 464 is provided at the bottom of the incubator 1, the liquid collecting bin 464 is connected to the inner cavity of the guide ring 463, a liquid extraction pipe 465 is fixed and connected to the upper part of the side wall of the liquid pipette box 46, and the other end of the liquid extraction pipe 465 is connected to the inner cavity of the liquid collecting bin 464.

[0042] By setting the above-mentioned structure, the nutrient solution sprayed and scattered in the culture box 1 will flow into the guide ring 463, and at this time, the driving shaft 431 rotates with the liquid suction blade 462 in the liquid suction box 46, and a suction effect will be generated on the side close to the liquid extraction pipe 465, so that the nutrient solution flowing into the guide ring 463 is sucked into the liquid suction box 46, and then flows back into the liquid storage tank 42 through the return pipe 461, so as to realize the recycling and reuse of the scattered nutrient solution and improve the energy-saving effect; and the rotation of the liquid suction blade 462 will continuously apply airflow thrust to the liquid storage tank 42 along the return pipe 461, so as to increase the falling pressure of the nutrient solution in the liquid storage tank 42, thereby accelerating the speed at which the nutrient solution is sprayed out along the atomizing nozzle 411, improving the spraying efficiency, reducing the working time of the driving motor 44, reducing energy consumption, and improving the energy-saving effect.

[0043] Reference Figure 5 , Figure 7 and Figure 8, wherein limiting grooves 5 are evenly spaced on the top of the culture plate 32, a driven plate 51 is rotatably connected in the limiting groove 5, the culture dish 33 is inserted in the limiting groove 5 and fits on the top of the driven plate 51, a positioning bearing 52 is provided at the bottom of the limiting groove 5, the bottom end of the rotating shaft of the driven plate 51 passes through the center of the positioning bearing 52 and is fixedly connected to a driven wheel 511, and the outer wall of the driven wheel 511 fits with the outer wall of the U-shaped member 31.

[0044] Through the arrangement of the above-mentioned structure, during the rotation of the culture plate 32, the driven wheel 511 will intermittently rotate in contact with the outer wall of the U-shaped member 31, thereby driving the driven plate 51 to rotate, so that the culture dish 33 suddenly obtains a rotation effect, and under the action of inertia, the stem cell tissue fluid and nutrient solution inside it are shaken, so that the stem cell tissue fluid is in uniform contact with the nutrient solution and oxygen, ensuring that each cell can obtain sufficient nutrition and oxygen supply, accelerating the cell reproduction efficiency, and improving the culture effect.

[0045] Reference Figure 5 , Figure 7 and Figure 8 , wherein the adsorption component includes a piston tube 6, which is fixed at the bottom of the limiting groove 5, the input end of the piston tube 6 is connected to the inner cavity of the limiting groove 5, a piston plate 61 is slidably connected in the piston tube 6, a return spring 611 is fixedly connected between the top of the piston plate 61 and the inner wall of the piston tube 6, an air guide pipe 612 is fixedly connected to the piston plate 61, three groups of magnetic blocks 62 are arranged at equal intervals on the top of the driven disk 51, the magnetic blocks 62 and the piston plate 61 repel each other magnetically, an adsorption groove 63 is opened in the driven disk 51, and the bottom end of the adsorption groove 63 is connected to the inner cavity of the limiting groove 5, and a one-way valve is arranged at the input and output ends of the piston tube 6 and in the air guide pipe 612.

[0046] It should be noted that the one-way valve in the input end of the piston tube 6 can only allow the gas in the limit groove 5 to enter the piston tube 6; the one-way valve in the output end of the piston tube 6 can only allow the gas in the piston tube 6 to be discharged outward; the one-way valve in the air guide tube 612 can only allow the gas in the upper cavity of the piston tube 6 to enter the lower cavity.

[0047] By setting the above structure, during the rotation of the driven wheel 511, the magnetic block 62 and the piston plate 61 will enter the repulsive area. Under the repulsive effect of the two, the piston plate 61 will move upward. Under the effect of gas resistance, the one-way valve of the air guide pipe 612 will be opened, so that the gas in the upper cavity of the piston tube 6 moves to the lower cavity. Then, when the magnetic block 62 and the piston plate 61 are separated from the repulsive area, the piston plate 61 will move downward and reset under the rebound effect of the reset spring 611. This compresses the airflow in the lower cavity of the piston tube 6, and opens the one-way valve at the input end of the piston tube 6 to discharge the airflow out of the piston tube 6. When the piston plate 61 moves downward, suction is generated in the upper cavity of the piston tube 6, so that the gas in the limiting groove 5 is sucked into the piston tube 6. This reciprocating motion will reduce the air pressure in the limiting groove 5, thereby generating an adsorption effect in the adsorption groove 63, so that the culture dish 33 fits tightly against the surface of the driven disk 51, thereby ensuring the stability of the culture dish 33 during the shaking culture process.

[0048] Reference Figure 1-Figure 8 In the present invention, when in use, the culture dish 33 is placed in the limiting groove 5, and then the sealing plate 8 is closed to seal the culture box 1, and then the sterilization lamp 2 is turned on to use the ultraviolet light emitted by it to disinfect the inner surface of the box to ensure a sterile environment and improve the culture effect. After the sterilization is completed, the sealing plug 71 can be opened, and the stem cell tissue fluid can be dripped into the culture dish 33 from the addition tube 7, and the driving motor 44 can be turned on intermittently, and the transmission effect of the pulley group 441, the driving shaft 431 and the bevel gear group 432 is coordinated to make the spraying tube 4 drive the friction wheel 451 to rotate, and then the friction effect is used to make the friction ring 311 drive the culture plate 32 to rotate, so as to drip the stem cell tissue fluid into the culture dishes 33 at different positions, and the operation is completely carried out in a sterile environment to ensure the culture effect of stem cells.

[0049] During the culture process, when nutrient solution needs to be added, the nutrient solution is first filled into the liquid storage tank 42, and the nutrient solution will enter the spray plate 41 along the spray pipe 4. Then, the drive motor 44 is turned on. At this time, the spray pipe 4 will drive the spray plate 41 to rotate, thereby generating centrifugal force in the spray plate 41, so that the nutrient solution is sprayed out in a rotational manner along the atomizing nozzles 411 at various locations. At the same time, the friction ring 311 drives the culture plate 32 to rotate, so that the culture dishes 33 at various locations will rotate and pass under the spray plate 41, so as to achieve a comprehensive and uniform spraying effect, which improves the efficiency of adding nutrient solution while ensuring that the quality of the nutrient solution added to the culture dishes 33 at various locations is the same, thereby improving the culture effect. Since the nutrient solution scattered in the incubator 1 by spraying will flow into the guide ring 463, at the same time, the driving shaft 431 rotates with the liquid suction blade 462 in the liquid suction box 46, and a suction effect will be generated on the side close to the liquid extraction tube 465, so that the nutrient solution flowing into the guide ring 463 is sucked into the liquid suction box 46, and then flows back into the liquid storage tank 42 through the return pipe 461, so as to realize the recycling and reuse of the scattered nutrient solution and improve the energy-saving effect; and the rotation of the liquid suction blade 462 will continuously apply airflow thrust to the liquid storage tank 42 along the return pipe 461, so as to increase the falling pressure of the nutrient solution in the liquid storage tank 42, thereby accelerating the speed at which the nutrient solution is sprayed out along the atomizing nozzle 411, thereby improving the spraying efficiency, reducing the working time of the driving motor 44, reducing energy consumption, and improving the energy-saving effect.

[0050] During the rotation of the culture dish 32, the driven wheel 511 will intermittently rotate in contact with the outer wall of the U-shaped member 31, thereby driving the driven dish 51 to rotate, so that the culture dish 33 suddenly obtains a rotating effect, and under the action of inertia, the stem cell tissue fluid and the nutrient solution inside thereof are shaken, so that the stem cell tissue fluid, the nutrient solution and the oxygen are evenly contacted, ensuring that each cell can obtain sufficient nutrition and oxygen supply, accelerating the cell reproduction efficiency and improving the culture effect; and during the rotation of the driven wheel 511, the magnetic block 62 and the piston plate 61 will enter the repulsive area, and under the repulsive action of the two, the piston plate 61 will move upward, and under the action of gas resistance, the one-way of the air guide tube 612 will be The valve is opened, so that the gas in the upper cavity of the piston tube 6 moves to the lower cavity. Then, when the magnetic block 62 and the piston plate 61 are separated from the repulsive area, the piston plate 61 will move down and reset under the rebound action of the reset spring 611, thereby compressing the airflow in the lower cavity of the piston tube 6 and opening the one-way valve at the input end of the piston tube 6 to discharge the airflow out of the piston tube 6. When the piston plate 61 moves downward, suction will be generated in the upper cavity of the piston tube 6, so that the gas in the limiting groove 5 is sucked into the piston tube 6. The reciprocating movement as above will reduce the air pressure in the limiting groove 5, thereby generating an adsorption effect in the adsorption groove 63, so that the culture dish 33 fits tightly on the surface of the driven disk 51, ensuring the stability of the culture dish 33 during the shaking culture process.

[0051] Embodiment 2:

[0052] Reference Figure 1-Figure 8 , which is basically the same as Example 1, and based on Example 1, a stem cell culture method is proposed, and the steps are as follows:

[0053] Step 1, place the culture dish 33 on the culture tray 32, and sterilize the inside of the incubator 1: place the culture dish 33 into the limiting groove 5, then close the sealing plate 8, seal the incubator 1, and then turn on the sterilization lamp 2, and use the ultraviolet light it emits to disinfect the inner surface of the box to ensure a sterile environment and improve the culture effect.

[0054] Step 2, dripping stem cells to be cultured into the culture dish 33: open the sealing plug 71, dripping stem cell tissue fluid into the culture dish 33 from the addition tube 7, and intermittently turning on the drive motor 44, cooperating with the transmission action of the pulley group 441, the drive shaft 431 and the bevel gear group 432, so that the spray pipe 4 drives the friction wheel 451 to rotate, and then using the friction effect, the friction ring 311 drives the culture plate 32 to rotate, so as to drip stem cell tissue fluid into the culture dishes 33 at different positions, and the operation is performed completely in a sterile environment to ensure the culture effect of the stem cells.

[0055] Step 3, adding nutrient solution to the culture dish 33 containing stem cells: fill the nutrient solution into the liquid storage tank 42, and the nutrient solution will enter the spray plate 41 along the spray pipe 4, then turn on the drive motor 44, cooperate with the transmission action of the pulley group 441, the drive shaft 431 and the bevel gear group 432, so that the spray pipe 4 drives the spray plate 41 to rotate, thereby generating centrifugal force in the spray plate 41, so that the nutrient solution is sprayed out in a rotational manner along the atomizing nozzles 411 at various locations, and cooperate with the rotation of the culture plate 32 to achieve a comprehensive and uniform spraying effect, which improves the efficiency of adding nutrient solution while ensuring that the quality of the nutrient solution added to each culture dish 33 is the same, thereby improving the culture effect.

[0056] Step 4: Shake the stem cells in the culture dish 33 to make them evenly contact with the nutrient solution: The driven wheel 511 intermittently rotates in contact with the outer wall of the U-shaped member 31 to drive the driven disk 51 to rotate, so that the culture dish 33 suddenly obtains a rotation effect. Under the action of inertia, the stem cell tissue fluid and the nutrient solution inside it are shaken, so that the stem cell tissue fluid is evenly contacted with the nutrient solution and oxygen, ensuring that each cell can obtain sufficient nutrition and oxygen supply, accelerating the cell reproduction efficiency and improving the culture effect.

[0057] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A stem cell culture device, comprising a culture box (1) and a germicidal lamp (2) fixed on the top of the inner cavity thereof, characterized in that: The middle part of the inner cavity of the incubator (1) is rotatably connected to a rotating disk (3), and the bottom part is fixedly connected to a U-shaped piece (31); the other end of the U-shaped piece (31) is fixedly connected to a culture dish (32); the culture dish (32) slides in contact with the inner wall of the incubator (1); a culture dish (33) is installed on the top of the culture dish (32); and an adsorption component for fixing the culture dish (33) is arranged at the bottom of the culture dish (32); the spray component is also included, and the spray component is arranged on the incubator (1); the spray component is used to transport nutrient solution into the culture dish (33).

2. A stem cell culture device according to claim 1, characterized in that: The spray assembly comprises a spray pipe (4), the spray pipe (4) being rotatably connected to the top of the inner cavity of the incubator (1), a spray disc (41) being fixedly connected to the outer wall of the spray pipe (4), and the spray disc (41) being communicated with the inner cavity of the spray pipe (4), a plurality of groups of atomizing nozzles (411) being fixedly arranged at equal intervals on the lower part of the outer wall of the spray disc (41), and the atomizing nozzles (411) being communicated with the inner cavity of the spray disc (41), a liquid storage tank (42) being fixedly connected to the top of the incubator (1), the top end of the spray pipe (4) extending through and above the top of the liquid storage tank (42), and the spray pipe (4) being communicated with the inner cavity of the liquid storage tank (42), and a centrifugal part for driving the spray pipe (4) to rotate is provided at the top of the incubator (1).

3. A stem cell culture device according to claim 2, characterized in that: The centrifugal part comprises a transmission box (43), the transmission box (43) is fixedly connected to the top of the liquid storage tank (42), a driving shaft (431) is rotatably connected to the transmission box (43), the top end of the spray pipe (4) passes through the transmission box (43), and the driving shaft (431) and the end of the spray pipe (4) close to each other are meshed and connected through a bevel gear set (432), and a driving motor (44) is fixedly connected to the top of the incubator (1), and the output shaft of the driving motor (44) is connected to the driving shaft (431) through a pulley set (441).

4. A stem cell culture device according to claim 2, characterized in that: The U-shaped members (31) are arranged in three groups at equal intervals, and a friction ring (311) is fixedly connected between the three groups of U-shaped members (31). A linkage shaft (45) is fixedly connected to the bottom of the spray plate (41), and the bottom end of the linkage shaft (45) passes through the annular cavity formed by the culture plate (32) and the culture dish (33) and extends to the friction ring (311). A friction wheel (451) is fixedly connected to the bottom end of the linkage shaft (45), and the friction wheel (451) is in contact with the side wall of the friction ring (311).

5. The stem cell culture device according to claim 3, characterized in that: The outer wall of the driving shaft (431) is rotatably connected to a liquid suction box (46), the lower part of the side wall of the liquid suction box (46) is fixedly connected to a return pipe (461), the other end of the return pipe (461) is fixedly connected to the upper part of the outer wall of the liquid storage tank (42), and the two ends of the return pipe (461) are respectively connected to the inner cavity of the liquid suction box (46) and the liquid storage tank (42). A liquid suction blade (462) is fixedly connected to the outer wall; a guide ring (463) is provided at the bottom of the inner cavity of the incubator (1); a liquid collecting bin (464) is provided at the bottom of the incubator (1); the liquid collecting bin (464) is communicated with the inner cavity of the guide ring (463); a liquid suction pipe (465) is fixed to and communicated with the upper part of the side wall of the liquid suction box (46); the other end of the liquid suction pipe (465) is communicated with the inner cavity of the liquid collecting bin (464).

6. The stem cell culture device according to claim 1, characterized in that: The top of the culture plate (32) is provided with limiting grooves (5) at equal intervals, a driven plate (51) is rotatably connected in the limiting groove (5), the culture dish (33) is inserted in the limiting groove (5) and fits on the top of the driven plate (51), a positioning bearing (52) is provided at the bottom of the limiting groove (5), the bottom end of the rotating shaft of the driven plate (51) passes through the center of the positioning bearing (52) and is fixedly connected to a driven wheel (511), and the outer wall of the driven wheel (511) fits with the outer wall of the U-shaped member (31).

7. A stem cell culture device according to claim 6, characterized in that: The adsorption assembly comprises a piston tube (6), the piston tube (6) is fixed at the bottom of the limiting groove (5), the input end of the piston tube (6) is communicated with the inner cavity of the limiting groove (5), a piston plate (61) is slidably connected in the piston tube (6), a return spring (611) is fixedly connected between the top of the piston plate (61) and the inner wall of the piston tube (6), an air guide tube (612) is fixedly connected to the piston plate (61), three groups of magnetic blocks (62) are arranged at equal intervals on the top of the driven disk (51), the magnetic blocks (62) and the piston plate (61) repel each other magnetically, an adsorption groove (63) is opened in the driven disk (51), and the bottom end of the adsorption groove (63) is communicated with the inner cavity of the limiting groove (5), and one-way valves are arranged at the input end and output end of the piston tube (6) and in the air guide tube (612).

8. The stem cell culture device according to claim 1, characterized in that: The top of the culture box (1) is fixedly connected with an addition tube (7), the top end of the addition tube (7) is threadedly connected with a sealing plug (71), and the output end of the addition tube (7) is located directly above the culture dish (33).

9. A stem cell culture device according to claim 8, characterized in that: A sealing plate (8) is plugged into the incubator (1), and a transparent window (81) is provided on the sealing plate (8).

10. A stem cell culture method, using a stem cell culture device according to any one of claims 1 to 9, characterized in that: Here are the steps: Step 1: Place the culture dish (33) on the culture tray (32) and sterilize the inside of the culture box (1); Step 2: adding stem cells to be cultured into the culture dish (33); Step 3, adding nutrient solution to the culture dish (33) containing stem cells; Step 4: Shake the stem cells in the culture dish (33) to make them evenly contact with the nutrient solution.

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