Experimental small animal forced swimming system
By combining observation and analysis and execution modules, direct stimulation modules, drowning prevention modules and environmental stimulation modules in the experimental small animal forced swimming system, intelligent and automated water temperature and water flow regulation are achieved, and the experimental time extension and the The problem of unclear observations improves experimental efficiency and accuracy and enhances the humanization of the system.
Patent Information
- Application Number
- CN202411287949.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-13
- Publication Date
- 2024-12-13
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing forced swimming devices for experimental animals cannot achieve intelligent and automated water temperature regulation and water flow surge regulation, resulting in a prolonged experimental time and unclear observations, making it difficult to finely display the movement trajectory and behavior of small animals.
An experimental small animal forced swimming system including observation and analysis and execution module, direct stimulation module, drowning prevention module and environmental stimulation module was designed. The animal's movement trajectory and vital signs were monitored through multiple sets of observation probes, and the animal's status was intelligently judged. Perform direct stimulation or water flow rotation to shorten the experiment time and stimulate the animals through weak currents and water tornadoes avoid overwork or hypoxia.
Intelligent and automated experimental control is realized, experimental time is shortened, experimental efficiency and accuracy is improved, animals are avoided overwork or hypoxia death, and the humanization of the system is enhanced.
Smart Images
Figure CN119111418A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of forced swimming experiments on experimental animals, in particular to a forced swimming system for experimental small animals. Background Art
[0002] Animal forced swimming experiment refers to placing animals in a confined swimming device with a certain water level to swim. The experimental animal forced swimming device is an animal experimental device that provides a forced swimming environment to achieve the experimental purpose of causing animals to be stressed and exhausted.
[0003] The existing forced swimming apparatus for experimental animals can realize some automatic functions, but many functions require manual participation, which brings great inconvenience to the experiment and is time-consuming and labor-intensive. In addition, the existing forced swimming apparatus for experimental animals cannot realize intelligent adjustment of functions such as water temperature regulation and water flow, which is not conducive to better establishing a forced swimming environment for experimental animals.
[0004] For example, a forced swimming system for experimental small animals disclosed in Chinese invention patent publication number CN117084190A realizes full, intelligent and automated control of the entire small animal forced swimming experiment process, specifically realizes the function of automatic water addition to adjust the water depth, the function of automatic water heating to adjust the water temperature, the function of automatic water flow surging to adjust the water flow, the function of automatic video monitoring of the forced swimming situation of the experimental small animals, and the function of automatic fishing of the experimental small animals when it is analyzed that the experimental small animals are in a state of exhaustion. The experimental small animals are fished out and stay on the water surface for a period of time. After the experimental small animals have rested, the experimental small animals are put into the water again to continue the forced swimming experiment, the function of automatic fishing of the experimental small animals when the experimental time is reached, the function of automatic water discharge, and the function of automatic cleaning and disinfection.
[0005] The above-mentioned device achieves the purpose of animal stress and exhaustion by providing a forced swimming environment, and realizes water temperature rise and water flow in an intelligent and automatic manner. However, when animals are in this swimming environment for too long and reach an adapted state, they often float on the water surface and remain still to conserve their energy. Simple water flow is difficult to further increase the stimulation to the small animals in the water, which forces the experiment time to be prolonged. In addition, although placing an observation head inside the experimental tube increases the observation range to a certain extent, the splashes when the small animals struggle can easily cause the observation head to wade in the water, resulting in unclear observation, making it difficult to quantify the data on the movement trajectory and fine behavior of the small animals. Summary of the invention
[0006] In view of the deficiencies of the prior art, the present invention provides a forced swimming system for experimental small animals, which solves the problems raised in the above-mentioned background technology.
[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: an experimental small animal forced swimming system, including an experimental table, on which four groups of open boxes for building a swimming platform are arranged, each group of the open boxes is provided with a swimming system for small animals forced to swim, the swimming system consists of an observation analysis and execution module, a direct stimulation module, an anti-drowning module and an environmental stimulation module, the observation analysis and execution module performs behavioral analysis based on the observation results to obtain execution decisions for the direct stimulation module, the anti-drowning module and the environmental stimulation module, and the observation analysis and execution module is electrically connected to the direct stimulation module, the anti-drowning module and the environmental stimulation module, respectively.
[0008] A further improvement of the technical solution of the present invention lies in that: four groups of closed plates connecting the vertices of different positions of the box are respectively arranged in each of the open boxes, and perfusion water pipes are respectively limitedly arranged in two groups of adjacent closed plates, and the other end of each group of perfusion water pipes is located in an experimental cylinder. The four groups of closed plates limit the experimental cylinder vertically in the open box, and a detachable force frame is respectively arranged on the axis where each group of perfusion water pipes is located in the experimental cylinder, and the inside of the experimental cylinder is coated with a super-slip coating to prevent small animals from climbing, and each group of perfusion water pipes is arranged with a plurality of groups of water perfusion holes in an array. In order to prevent small animals from climbing or hanging on the cylinder wall during the forced swimming experiment, the present invention specifically coats the inner wall of the experimental cylinder with a super-slip coating. The super-slip coating has excellent anti-stickiness and easy-to-clean properties, and is intended to reduce the friction coefficient of the inner wall of the experimental cylinder, making it difficult for animals to attach; and a force frame installed at the perfusion water pipe is specifically provided as a necessary climbing path for small animals. This design is intended to measure whether there is active escape behavior when the small animals have hope of survival.
[0009] A further improvement of the technical solution of the present invention is that: the observation analysis and execution module includes a slide seat arranged between two other sets of adjacent closed plates, a slider for horizontal movement is arranged between the two slide seats, a connecting rod is connected to the top of the slider by a movable universal ball, the other end of the connecting rod is connected to an observation device, and a plurality of observation probes are arranged at the front end of the observation device;
[0010] The multiple groups of observation probes include monitoring probes for observing the overall situation of the water surface of the experimental tube, infrared 3D monitoring probes for observing the thermal image of experimental small animals, and capture probes for motion capture.
[0011] A further improvement of the technical solution of the present invention is that the observation, analysis and execution module also includes an analysis unit, which uses a monitoring probe to identify animals and complete real-time tracking of animals, and the analysis unit generates a swimming trajectory based on a time series based on the image data taken by the monitoring probe, and the analysis unit generates a vital sign based on a time series based on the infrared D detection probe, and the analysis unit generates a motion trajectory based on a time series based on the capture probe. There are multiple groups of observation probes to complete the real-time tracking of small animals, the monitoring of the vital signs of small animals, and the monitoring of the movement trajectories of small animals at different times. Then, the monitored images are used to complete the feature extraction of the monitored images based on the HOG algorithm. The generation of the swimming trajectory based on the time series is to splice the image features taken at adjacent times, and use the monitoring probe as the coordinate origin to calculate the vector change between the coordinate origin and the position of the image feature pixel, which is finally displayed on the display screen. Similarly, the thermal radiation brightness of the animal captured by the infrared D detection probe is spliced based on the time series to complete the thermal radiation change of the animal in the swimming state, so as to observe the specific changes in the animal's vital signs. The specific time of the monitoring image bracket taken at adjacent times is s, so the distance between the animal position photographed by the monitoring probe in the next second and the animal position photographed by the monitoring probe in the previous second is taken as the average movement speed of the animal v ’ mm / s, the resulting v ’ > The preset speed is considered as the animal's active movement state, v ’ ≤preset speed is regarded as the animal's inert motion state. When the analysis shows an inert motion state, the execution unit sends a start signal to the direct stimulation module to complete the direct stimulation of the animal, aiming to stimulate the animal's survival instinct; when the animal's thermal radiation amount obtained by the infrared D monitoring probe is greater than the minimum thermal radiation amount of vital signs, it is regarded as the animal has the ability to move. When the animal's thermal radiation amount obtained by the infrared D monitoring probe is ≤the minimum thermal radiation amount of vital signs, the execution unit sends a start signal to the anti-drowning module for emergency drainage.
[0012] A further improvement of the technical solution of the present invention is that two groups of waterproof plates are symmetrically arranged at the bottom of the observation equipment, and the front and rear sections of each group of waterproof plates are fixedly connected with extension plates, and each of the extension plates is slidably installed in a track groove in the arc track, and an arc guide rod is passed through the track groove, and a reset spring is provided on the upper limit sleeve of the arc guide rod, and a positioning plate is fixedly installed on the top of the arc track, and the positioning plate is slidably installed on the adjustment seat, and a positioning rod is slidably installed on the positioning plate, and the adjustment seat is welded to a protective shell installed on the top of the observation equipment.
[0013] Furthermore, waterproof plates are arranged on both sides of the bottom of the observation equipment, and cooperate with the protective shell on the top to complete the overall protection of the observation equipment, so as to prevent the observation equipment or the observation probe from getting wet due to animals hitting the water surface under stress. Because the movable setting of the universal ball enables the observation equipment to be adjusted to a certain pitch angle, in this case, the position of the waterproof plate set at the bottom of the observation equipment also needs to be adjusted accordingly, so the positioning plate slides on the adjustment seat.
[0014] A further improvement of the technical solution of the present invention is that in order to ensure that the observation equipment can be disassembled and installed, thereby facilitating the rapid conduct of the experiment, the positioning rod fixes the protective shell through a plurality of groups of positioning holes arranged in an array on the protective shell, and limiting openings are provided at the bottom of the left and right ends of the protective shell, and the waterproof plate is connected to the protective shell through the limiting openings.
[0015] A further improvement of the technical solution of the present invention is that: the direct stimulation module includes conductive sheets respectively installed at the top of each group of experimental tubes, each group of the conductive sheets is in contact with the experimental tubes, and a connecting joint is fixedly installed on the top of the conductive sheets, and each group of the connecting joints is connected to a wire, and the other two ends of the wire are respectively connected to a small AC power generation device installed on the experimental table, and the control switch of the small AC power generation device is electrically connected by an execution unit in the observation and analysis and execution module, and each group of the experimental tubes is made of conductive material. The control switch of the small AC power generation device is started and stopped after the execution unit issues an execution command. When the small AC power generation device is turned on, a stable weak current is emitted, which is transmitted to the conductive sheet by the wire. Due to the conductivity of the experimental tube and its contact with the conductive sheet, and water as a good conductor, the animal in a passive state is directly stimulated by the weak current, which makes the animal stressed again, avoids the animal from being still for a long time and conserves physical strength, thereby discovering the behavioral changes and physiological changes of the animal after experiencing a secondary stress state.
[0016] A further improvement of the technical solution of the present invention is that the environmental stimulation module includes a stainless steel transmission wheel fixedly arranged at the bottom of each group of experimental cylinders, a tooth plate is fixedly installed on each of the stainless steel transmission wheels through a recess, a gear meshing with each group of tooth plates is arranged between the four tooth plates, the gear is connected to a drive shaft through a connecting key, and the other end of the drive shaft is connected to a drive motor through a coupling.
[0017] A further improvement of the technical solution of the present invention is that: centrifugal rods are arranged in an array on the top of each of the stainless steel transmission wheels, and a movable sleeve keyed thereto is arranged in a vertical array on each of the centrifugal rods, and a telescopic impact rod is arranged on the outer wall of each of the movable sleeves, and a stabilizing rod is arranged between adjacent movable sleeves. The difference between the environmental stimulation module and the direct stimulation module is that the stainless steel transmission wheel drives the experimental cylinder to rotate, so that the water flow inside the experimental cylinder produces a waterspout, causing the animals in the water to shake. Compared with the direct stimulation module, the method is milder, avoiding the animals from excessive stress and death, and increasing the physical energy consumption of the animals, so that the animals can face the inescapable emergency environment more quickly, shortening the time required for the experiment.
[0018] A further improvement of the technical solution of the present invention is that the anti-drowning module includes a capture net arranged inside each group of experimental cylinders, and a sealing plate is arranged directly below each group of the capture nets, each group of the sealing plates are provided with drainage holes in an array, each of the drainage holes is provided with a sealing plug, and a sealing rod with the other end connected to a connecting plate is slidably installed at the bottom of each sealing plug, and a hydraulic rod fixedly installed at the bottom of the sealing plate is provided in the middle of the connecting plate.
[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: the monitoring of the animal's motion trajectory, vital signs and action trajectory based on the time series is completed through the observation analysis and execution modules, and whether there is a negative state or a situation of conserving physical strength is intelligently and automatically judged according to the animal's real-time motion speed and motion state, and direct stimulation is given to stimulate the survival instinct, shorten the experimental time, improve the experimental efficiency, so that the experimental scope and experimental accuracy of the system are further improved, and the drainage operation is quickly completed when the animal's vital signs are abnormal, so as to avoid the animal's death due to overwork or lack of oxygen, making the experimental system more humane;
[0020] After the small AC generator is turned on, a stable weak current is emitted, which is transmitted to the conductive sheet by the wire. Because the experimental tube is conductive and in contact with the conductive sheet, and water is a good conductor, the animals in a passive state are directly stimulated by the weak current, which re-stresses the animals and prevents them from being still for a long time to conserve their energy, thus discovering the behavioral and physiological changes of the animals after experiencing a secondary stress state.
[0021] The experimental cylinder is driven to rotate by a stainless steel transmission wheel, so that the water flow inside the experimental cylinder produces a waterspout, causing the animals in the water to shake. Compared with the direct stimulation module, this method is gentler and can prevent the animals from dying due to excessive stress. It also increases the animals' physical energy consumption, allowing them to face the inescapable emergency environment more quickly and shorten the time required for the experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a schematic diagram of the overall structure of the forced swimming system for experimental small animals;
[0023] Figure 2 Provides a bird's-eye view of the forced swimming system for experimental small animals;
[0024] Figure 3 This is a schematic diagram of the structure of the environmental stimulation module in the forced swimming system for experimental small animals;
[0025] Figure 4 This is a bottom view of the partial structure of the environmental stimulation module in the experimental small animal forced swimming system;
[0026] Figure 5 This is a schematic diagram of the structure of the experimental cylinder in the forced swimming system for experimental small animals;
[0027] Figure 6 This is a front view of the anti-drowning module in the forced swimming system for experimental small animals;
[0028] Figure 7 This is a structural diagram of the open box in the forced swimming system for experimental small animals;
[0029] Figure 8 This is a schematic diagram of the structure of the observation, analysis and execution modules in the forced swimming system for experimental small animals;
[0030] Fig. 9 This is a schematic diagram of the observation, analysis and execution modules in the experimental small animal forced swimming system;
[0031] Fig.10 This is a schematic diagram of the swimming system in the forced swimming system for experimental small animals;
[0032] Fig.11 This is a diagram of the animal movement trajectory in the experimental animal forced swimming system.
[0033] In the figure: 100, experimental table; 200, open box; 300, observation analysis and execution module; 400, direct stimulation module; 500, drowning prevention module; 600, environmental stimulation module; 201, closing plate; 202, water injection pipe; 203, experimental cylinder; 204, force frame; 205, water injection hole; 301, sliding seat; 302, slider; 303, universal ball; 304, connecting rod; 305, observation equipment; 306, observation probe; 307, waterproof board; 308, extension board; 309, arc track; 310, arc guide rod; 311, reset spring; 312, arc guide rod; 313, return spring; 314, arc guide rod; 315, return spring; 316, arc guide rod; 317, return spring; 318, arc guide rod; 319, return spring; 320, arc guide rod; 321, return spring; 322, arc guide rod; 323, return spring; 324, return spring; 325, return spring; 326, return spring; 327, return spring; 328, return spring; 329, return spring; 330, return spring; 331, return spring; 332, return spring; 333, return spring; 334, return spring; 335, return spring; 336, return spring; 337, return spring; 338, return spring; 339, return spring; 340, return spring; 341, return spring; 342, return spring; 343, return spring; 344, return spring; 345, return spring; 346, return spring; 347, return spring; 348, return spring; 349, return spring; 350, return spring; 351 12. Positioning plate; 313. Adjusting seat; 314. Positioning plug; 315. Protective shell; 316. Positioning hole; 401. Conducting sheet; 402. Connecting joint; 403. Wire; 404. Small AC power generation equipment; 501. Capture net; 502. Sealing plate; 503. Sealing plug; 504. Connecting plate; 505. Sealing rod; 506. Hydraulic rod; 601. Stainless steel transmission wheel; 602. Tooth plate; 603. Gear; 604. Drive shaft; 605. Drive motor; 606. Centrifugal rod; 607. Movable sleeve; 608. Telescopic impact rod; 609. Stabilizing rod. DETAILED DESCRIPTION
[0034] Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise specified.
[0035] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.
[0036] In addition, in order to better illustrate the present application, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present application can also be implemented without certain specific details. In some examples, methods, means, and elements well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present application.
[0037] Reference Figure 1 - Fig.11 , the present invention provides three technical solutions:
[0038] Embodiment 1:
[0039] A small animal forced swimming system comprises a test bench 100. Four groups of open boxes 200 for building a swimming platform are arranged on the test bench 100. A swimming system for small animals forced swimming is arranged in each group of open boxes 200. The swimming system comprises an observation analysis and execution module 300, a direct stimulation module 400, an anti-drowning module 500 and an environmental stimulation module 600. The observation analysis and execution module 300 performs behavior analysis based on the observation results to obtain execution decisions of the direct stimulation module 400, the anti-drowning module 500 and the environmental stimulation module 600. The observation analysis and execution module 300 is electrically connected to the direct stimulation module 400, the anti-drowning module 500 and the environmental stimulation module 600, respectively.
[0040] Each open box 200 is respectively provided with four groups of closed plates 201 connecting the vertices at different positions of the box, and two groups of adjacent closed plates 201 are respectively provided with perfusion water pipes 202 for limiting positions. The other end of each group of perfusion water pipes 202 is located in an experimental cylinder 203. The four groups of closed plates 201 vertically limit the experimental cylinder 203 in the open box 200, and each group of perfusion water pipes 202 is respectively provided with a detachable force frame 204 on the axis where the experimental cylinder 203 is located, and the interior of the experimental cylinder 203 is coated with an ultra-slip coating to prevent small animals from climbing, and each group of perfusion water pipes 202 is arranged with a plurality of groups of water holes 205 in an array.
[0041] In order to prevent the small animals from climbing or hanging on the cylinder wall during the forced swimming experiment, the present invention specifically coats the inner wall of the experimental cylinder 203 with a super-slip coating, which has excellent anti-stick and easy-to-clean properties, and is intended to reduce the friction coefficient of the inner wall of the experimental cylinder 203, making it difficult for the animals to adhere;
[0042] Moreover, a force frame 204 installed at the irrigation pipe 202 is specifically provided as a necessary climbing path for small animals. This design is intended to measure whether there is active escape behavior when the small animals have hope of survival.
[0043] Embodiment 2:
[0044] On the basis of the first embodiment: the observation analysis and execution module 300 includes a slide seat 301 disposed between two other sets of adjacent closed plates 201, a slider 302 for horizontal movement is disposed between the two slide seats 301, a connecting rod 304 is connected to the top of the slider 302 by a movable universal ball 303, an observation device 305 is connected to the other end of the connecting rod 304, and a plurality of observation probes 306 are disposed at the front end of the observation device 305;
[0045] The multiple groups of observation probes 306 include monitoring probes for observing the overall situation of the water surface of the experimental tube 203, infrared 3D monitoring probes for observing the thermal image of experimental small animals, and capture probes for motion capture.
[0046] The observation, analysis and execution module 300 also includes an analysis unit, which uses the monitoring probe to identify animals and complete real-time tracking of animals. The analysis unit generates a swimming trajectory based on a time series based on the image data taken by the monitoring probe. The analysis unit generates vital signs based on a time series based on the infrared 3D detection probe. The analysis unit generates a motion trajectory based on a time series based on the capture probe.
[0047] There are multiple groups of observation probes 306 to respectively complete the real-time tracking of small animals, the monitoring of the vital signs of small animals, and the monitoring of the movement trajectories of small animals at different times, and then complete the feature extraction of the monitored images based on the HOG algorithm. The generation of the swimming trajectory based on the time series is to splice the image features taken at adjacent times, and use the monitoring probe as the coordinate origin to calculate the vector change Δθ between the coordinate origin and the position of the image feature pixel, and finally display it on the display screen. Similarly, the thermal radiation brightness of the animal captured by the infrared 3D detection probe is spliced based on the time series to complete the thermal radiation change of the animal in the swimming state, so as to observe the specific changes of the animal's vital signs.
[0048] Furthermore, the specific time of the monitoring image bracket taken at adjacent times is 1 second, so the distance between the animal position photographed by the monitoring probe in the next second and the animal position photographed by the monitoring probe in the previous second is taken as the average movement speed of the animal v ’ mm / s, the resulting v ’ > The preset speed is considered as the animal's active movement state, v ’ ≤ the preset speed is regarded as the inertial motion state of the animal. When the inertial motion state is obtained through analysis, the execution unit sends a start signal to the direct stimulation module 400 to complete the direct stimulation of the animal, aiming to stimulate the survival instinct of the animal;
[0049] Furthermore, when the thermal radiation of the animal obtained by the infrared 3D monitoring probe is greater than the minimum thermal radiation of vital signs, the animal is deemed to have the ability to move, and when the thermal radiation of the animal obtained by the infrared 3D monitoring probe is less than or equal to the minimum thermal radiation of vital signs, the execution unit sends a start signal to the anti-drowning module 500 to drain water urgently;
[0050] Therefore, it can be seen from the above that the present invention uses the observation analysis and execution module 300 to complete the monitoring of the animal's movement trajectory, vital signs and action trajectory based on the time series, and intelligently and automatically judges whether there is a negative state or a situation of conserving physical strength according to the animal's real-time movement speed and movement state, and directly stimulates the survival instinct, shortens the experimental time, improves the experimental efficiency, and further improves the experimental scope and experimental accuracy of the system. When the animal's vital signs are abnormal, the drainage operation is quickly completed to avoid the animal's death from overwork or lack of oxygen, making the experimental system more humane.
[0051] Two groups of waterproof plates 307 are symmetrically arranged at the bottom of the observation device 305. The front and rear sections of each group of waterproof plates 307 are fixedly connected with extension plates 308. Each extension plate 308 is slidably installed in a track groove in the arc track 309. An arc guide rod 310 is passed through the track groove, and a return spring 311 is provided on the upper limit sleeve of the arc guide rod 310. A positioning plate 312 is fixedly installed on the top of the arc track 309. The positioning plate 312 is slidably installed on the adjustment seat 313, and a positioning rod 314 is slidably installed on the positioning plate 312. The adjustment seat 313 is welded to a protective shell 315 installed on the top of the observation device 305.
[0052] Furthermore, waterproof plates 307 are arranged on both sides of the bottom of the observation device 305, and cooperate with the protective shell 315 on the top to provide overall protection for the observation device 305, so as to prevent the animal from hitting the water surface under stress and causing water to enter the observation device 305 or the observation probe 306. Because the movable setting of the universal ball 303 enables the observation device 305 to adjust a certain pitch angle, in this case, the position of the waterproof plate 307 set at the bottom of the observation device 305 also needs to be adjusted accordingly, so the positioning plate 312 slides on the adjustment seat 313.
[0053] In order to ensure that the observation equipment 305 can be disassembled and installed, thereby facilitating the rapid conduct of the experiment, the positioning rod 314 is fixed to the protective shell 315 by a plurality of groups of positioning holes 316 arranged in an array on the protective shell 315, and limiting openings are set at the bottom of the left and right ends of the protective shell 315, and the waterproof plate 307 is connected to the protective shell 315 through the limiting openings.
[0054] Embodiment three:
[0055] On the basis of Example 1 and Example 2: the direct stimulation module 400 includes conductive sheets 401 respectively installed at the top of each group of experimental tubes 203, each group of conductive sheets 401 is in contact with the experimental tubes 203, and a connecting connector 402 is fixedly installed on the top of the conductive sheet 401, and each group of connecting connectors 402 is connected to a wire 403, and the other two ends of the wire 403 are respectively connected to a small AC power generation device 404 installed at the experimental table 100, and the control switch of the small AC power generation device 404 is electrically connected by the execution unit in the observation and analysis and execution module 300, and each group of experimental tubes 203 is made of conductive material.
[0056] Furthermore, the control switch of the small AC power generation device 404 is started and stopped after the execution unit issues an execution command. When the small AC power generation device 404 is turned on, it emits a stable weak current, which is transmitted to the conductive sheet 401 by the wire 403. Due to the conductivity of the experimental tube 203 and its contact with the conductive sheet 401, and the water being a good conductor, the animals in the passive state are directly stimulated by the weak current, which makes the animals stressed again, avoids the animals from being still for a long time and conserves their physical strength, thereby discovering the behavioral changes and physiological changes of the animals after experiencing a secondary stress state.
[0057] The environmental stimulation module 600 includes a stainless steel transmission wheel 601 fixedly arranged at the bottom of each group of experimental cylinders 203, and a tooth plate 602 is fixedly installed on each stainless steel transmission wheel 601 through a recess. A gear 603 meshing with each group of tooth plates 602 is arranged between the four groups of tooth plates 602, and the gear 603 is connected to a driving shaft 604 through a connecting key, and the other end of the driving shaft 604 is connected to a driving motor 605 through a coupling.
[0058] Centrifugal rods 606 are arranged in an array on the top of each stainless steel transmission wheel 601, and movable sleeves 607 connected thereto by keys are arranged vertically in an array on each centrifugal rod 606. A telescopic impact rod 608 is arranged on the outer wall of each movable sleeve 607, and a stabilizing rod 609 is arranged between adjacent movable sleeves 607.
[0059] Furthermore, the difference between the environmental stimulation module 600 and the direct stimulation module 400 is that the stainless steel transmission wheel 601 drives the experimental tube 203 to rotate, so that the water flow inside the experimental tube 203 produces a waterspout, causing the animals in the water to shake. Compared with the direct stimulation module 400, the method is gentler, avoiding the animals from excessive stress and death, and increasing the physical energy consumption of the animals, so that the animals can face the inescapable emergency environment more quickly, shortening the time required for the experiment.
[0060] The anti-drowning module 500 includes a capture net 501 arranged inside each group of experimental cylinders 203, and a sealing plate 502 is arranged directly below each group of capture nets 501, each group of sealing plates 502 is provided with drainage holes in an array, each drainage hole is provided with a sealing plug 503, and a sealing rod 505 is slidably installed at the bottom of each sealing plug 503, and the other end of the sealing rod 505 is connected to a connecting plate 504, and a hydraulic rod 506 fixedly installed at the bottom of the sealing plate 502 is provided in the middle of the connecting plate 504.
[0061] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0062] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A forced swimming system for experimental small animals, comprising an experimental table (100), characterized in that: The experimental platform (100) is provided with four groups of open boxes (200) for building a swimming platform. A swimming system for forced swimming of small animals is provided in each group of the open boxes (200). The swimming system comprises an observation analysis and execution module (300), a direct stimulation module (400), an anti-drowning module (500) and an environmental stimulation module (600). The observation analysis and execution module (300) performs a behavior analysis based on the observation results to obtain an execution decision for executing the direct stimulation module (400), the anti-drowning module (500) and the environmental stimulation module (600). The observation analysis and execution module (300) is electrically connected to the direct stimulation module (400), the anti-drowning module (500) and the environmental stimulation module (600), respectively.
2. The experimental small animal forced swimming system according to claim 1, characterized in that: Each of the open boxes (200) is provided with four groups of closed plates (201) connected to the vertices of different positions of the boxes, and two groups of adjacent closed plates (201) are provided with injection water pipes (202) in a limited manner, and the other end of each group of the injection water pipes (202) is located in an experimental cylinder (203). The four groups of closed plates (201) vertically limit the experimental cylinder (203) in the open box (200), and each group of the injection water pipes (202) is provided with a detachable force frame (204) on the axis where the injection water pipes (202) are located in the experimental cylinder (203), and the interior of the experimental cylinder (203) is coated with a super-slip coating to prevent small animals from climbing, and each group of the injection water pipes (202) is provided with a plurality of groups of injection holes (205) arranged in an array.
3. The experimental small animal forced swimming system according to claim 2, characterized in that: The observation analysis and execution module (300) comprises a slide seat (301) arranged between two other groups of adjacent closed plates (201), a slider (302) for horizontal movement is arranged between the two slide seats (301), a connecting rod (304) is connected to the top of the slider (302) by a movable universal ball (303), the other end of the connecting rod (304) is connected to an observation device (305), and a plurality of observation probes (306) are arranged at the front end of the observation device (305); The plurality of observation probes (306) include a monitoring probe for observing the overall situation of the water surface of the experimental tube (203), an infrared 3D monitoring probe for observing the thermal image of the experimental small animals, and a capture probe for motion capture.
4. The experimental small animal forced swimming system according to claim 3, characterized in that: The observation, analysis and execution module also includes an analysis unit, which uses a monitoring probe to identify animals and complete real-time tracking of animals. The analysis unit generates a swimming trajectory based on a time series using the image data taken by the monitoring probe. The analysis unit generates vital signs based on a time series using an infrared 3D detection probe. The analysis unit generates an action trajectory based on a time series using a capture probe.
5. The experimental small animal forced swimming system according to claim 3, characterized in that: Two groups of waterproof plates (307) are symmetrically arranged at the bottom of the observation device (305), and the front and rear ends of each group of waterproof plates (307) are fixedly connected to extension plates (308), and each extension plate (308) is slidably installed in a track groove in the arc track (309), and an arc guide rod (310) is passed through the track groove, and the upper limit sleeve of the arc guide rod (310) is provided with a return spring (311), and a positioning plate (312) is fixedly installed on the top of the arc track (309), and the positioning plate (312) is slidably installed on the adjustment seat (31 3), and a positioning rod (314) is slidably mounted on the positioning plate (312), and the adjustment seat (313) is welded to a protective shell (315) mounted on the top of the observation device (305).
6. The experimental small animal forced swimming system according to claim 5, characterized in that: The positioning plug rod (314) fixes the protective shell (315) through a plurality of positioning holes (316) arranged in an array on the protective shell (315); limiting openings are provided at the bottom of the left and right ends of the protective shell (315); and the waterproof plate (307) is plugged into the protective shell (315) through the limiting openings.
7. The experimental small animal forced swimming system according to claim 2, characterized in that: The direct stimulation module (400) comprises conductive sheets (401) respectively installed at the top of each group of experimental tubes (203), each group of the conductive sheets (401) is in contact with the experimental tubes (203), and a connecting connector (402) is fixedly installed on the top of the conductive sheets (401), and each group of the connecting connectors (402) is connected to a wire (403), and the other two ends of the wire (403) are respectively connected to a small AC power generation device (404) installed on the experimental table (100), and the control switch of the small AC power generation device (404) is electrically connected to the execution unit in the observation analysis and execution module (300), and each group of the experimental tubes (203) is made of conductive material.
8. The experimental small animal forced swimming system according to claim 2, characterized in that: The environmental stimulation module (600) includes a stainless steel transmission wheel (601) fixedly arranged at the bottom of each group of experimental cylinders (203), a toothed plate (602) is fixedly installed on each of the stainless steel transmission wheels (601) through a notch, and a gear (603) meshing with each group of toothed plates (602) is arranged between the four toothed plates (602), the gear (603) is connected to a driving shaft (604) through a connecting key, and the other end of the driving shaft (604) is connected to a driving motor (605) through a coupling.
9. The experimental small animal forced swimming system according to claim 8, characterized in that: Centrifugal rods (606) are arranged in an array on the top of each of the stainless steel transmission wheels (601), and movable sleeves (607) connected thereto by keys are arranged vertically in an array on each of the centrifugal rods (606). A telescopic impact rod (608) is arranged on the outer wall of each movable sleeve (607), and a stabilizing rod (609) is arranged between adjacent movable sleeves (607).
10. The experimental small animal forced swimming system according to claim 1, characterized in that: The drowning prevention module (500) includes a capture net (501) arranged inside each group of experimental cylinders (203), and a sealing plate (502) is arranged directly below each group of the capture net (501), each group of the sealing plates (502) is provided with drainage holes in an array, each of the drainage holes is provided with a sealing plug (503), and a sealing rod (505) is slidably installed at the bottom of each sealing plug (503), the other end of which is connected to a connecting plate (504), and a hydraulic rod (506) fixedly installed at the bottom of the sealing plate (502) is arranged in the middle of the connecting plate (504).