A new pineapple mealybug attractant and its application

Abstract

The present invention discloses a new pineapple mealybug attractant and its application. The attractant is composed of the following components in parts by weight: 5-6 parts of 3-hexanone, 10-12 parts of 3-hexanol, 10-12 parts of 2-hexanol, 2-3 parts of 3-ethylacetophenone, and 2-3 parts of 2,2-bis(2-tetrahydrofuryl)propane. The new pineapple mealybug attractant can be used in the prevention and control of new pineapple mealybug attractants, which is beneficial for reducing the negative impact of chemical control on crops such as sisal, pineapple, pumpkin, banana, and mangosteen.

Description

Technical Field

[0001] The invention relates to the technical field of prevention and control of new pineapple gray mealybugs, in particular to a new pineapple gray mealybug attractant and application thereof. Background Art

[0002] The pineapple mealybug, Dysmicoccus neobrevipes, is an invasive pest in my country. It belongs to the genus Dysmicoccus, family Pseudococcidae, order Hemiptera. It threatens 76 species of tropical and subtropical agricultural and forestry crops from 39 families, including sisal, pineapple, pumpkin, banana, and mangosteen. It is a serious threat to sisal, fruits, vegetables, and flowers in its breeding areas. It not only sucks nutrients from its host but also transmits various plant viruses, such as sisal purple leafroll disease and pineapple wilt disease, causing significant economic losses.

[0003] Currently, chemical control is the primary method for the prevention and control of the new pineapple mealybug. However, the insect's high reproductive capacity, frequent outbreaks, hidden nature, and evolving resistance to pesticides significantly increase the difficulty of prevention and control. Therefore, the need to safely and effectively control the new pineapple mealybug and explore new avenues for its prevention and control has become an urgent issue.

[0004] Attractants are active substances produced by plants or artificially synthesized that have a behavioral attractant effect on specific insects. They are effective biological control agents and are widely used in the production of organic agricultural products such as rice, corn, cotton, fruit trees, and vegetables. However, there are currently few reports on the prevention and control of new pineapple gray mealybugs. Summary of the Invention

[0005] The invention discloses a new pineapple gray mealybug attractant and application thereof. The new pineapple gray mealybug attractant can be applied to the prevention and treatment of the new pineapple gray mealybug attractant, and is beneficial to reducing the negative impact of chemical prevention and treatment on crops such as sisal, pineapple, pumpkin, banana and mangosteen.

[0006] To achieve the above object, the technical solution of the present invention is:

[0007] A new pineapple mealybug attractant is composed of the following components in parts by weight: 5-6 parts of 3-hexanone, 10-12 parts of 3-hexanol, 10-12 parts of 2-hexanol, 2-3 parts of 3-ethylacetophenone, and 2-3 parts of 2,2-bis(2-tetrahydrofuryl)propane.

[0008] Preferably, the new pineapple mealybug attractant described above is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone, and 2.5 parts of 2,2-di(2-tetrahydrofuryl)propane.

[0009] The present invention also provides the use of the above-mentioned new pineapple gray mealybug attractant in preventing and controlling the new pineapple gray mealybug.

[0010] Furthermore, the attractant is mixed with a carrier, or adsorbed in the carrier, or sprayed on weeds in the sisal field.

[0011] Furthermore, the carrier is one of rubber, polyethylene sustained-release bottle, liquid paraffin and activated carbon.

[0012] Furthermore, the concentration of the attractant in liquid paraffin is 10-1000 ng / μL.

[0013] The present invention is based on the study of volatile substances of the host of the new pineapple gray mealybug, and obtains an attractant containing 3-hexanone, 1,3-hexanol, 2-hexanol, 3-ethylacetophenone, and 2,2-bis(2-tetrahydrofuryl)propane. The attractant has a significant attracting effect on the new pineapple gray mealybug, can be used in the prevention and control of the new pineapple gray mealybug attractant, and is beneficial to reducing the negative impact of chemical control on crops such as sisal, pineapple, pumpkin, banana and mangosteen. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The figures are the attracting effects of Examples 1-4 and Comparative Examples 1-3 on male Pseudomonas aeruginosa.

[0015] Figure 2 The figures are the attracting effects of Examples 1-4 and Comparative Examples 1-3 on female Pseudomonas aeruginosa. DETAILED DESCRIPTION

[0016] The present invention will be further described below with reference to specific examples, but the protection scope of the present invention is not limited to the following examples.

[0017] In this example, 3-hexanone, 3-hexanol, and 2-hexanol were collected from pineapple using dynamic headspace adsorption. 3-ethylacetophenone and 2,2-bis(2-tetrahydrofuryl)propane were collected from sisal using dynamic headspace adsorption. Their compositions were determined by comparison with standard samples purchased from Shanghai MacLean Biochemical Technology Co., Ltd. The liquid paraffin oil used was analytical grade S10187, SCRC. Example

[0018] A new pineapple gray mealybug attractant is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone, and 2.5 parts of 2,2-bis(2-tetrahydrofuryl)propane.

[0019] Prepare 1 μg / μL 3-hexanone solution, 1 μg / μL 3-hexanol solution, 1 μg / μL 2-hexanol solution, 1 μg / μL 3-ethylacetophenone solution, and 1 μg / μL 2,2-di(2-tetrahydrofuryl)propane solution respectively, and use liquid paraffin as the dilution solvent. Stir until the mixture is uniform, and then dilute to prepare a 10 ng / μL attractant solution. The carrier of the attractant is liquid paraffin. Example

[0020] Example 2 is basically the same as Example 1, but is finally diluted to prepare an attractant solution of 100 ng / μL, and the carrier of the attractant is liquid paraffin. Example

[0021] Example 2 is basically the same as Example 1, but is finally diluted to prepare an attractant solution of 1000 ng / μL, and the carrier of the attractant is liquid paraffin. Example

[0022] A new pineapple mealybug attractant is composed of the following components in parts by weight: 6 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2 parts of 3-ethylacetophenone, and 2 parts of 2,2-bis(2-tetrahydrofuryl)propane.

[0023] Prepare 1 μg / μL 3-hexanone solution, 1 μg / μL 3-hexanol solution, 1 μg / μL 2-hexanol solution, 1 μg / μL 3-ethylacetophenone solution, and 1 μg / μL 2,2-di(2-tetrahydrofuryl)propane solution respectively, and use liquid paraffin as the dilution solvent. Stir until the mixture is uniform, and then dilute to prepare a 10 ng / μL attractant solution. The carrier of the attractant is liquid paraffin.

[0024] Comparative Example 1

[0025] The attractant consisted of 3-hexanol and 2-hexanol in a weight ratio of 1:1.

[0026] Prepare 1 μg / μL 3-hexanol solution and 1 μg / μL 2-hexanol solution respectively, use liquid paraffin as dilution solvent, stir until mixed evenly, and then dilute with liquid paraffin to prepare 10 ng / μL attractant solution.

[0027] Comparative Example 2

[0028] The attractant is composed of 3-hexanone and 3-ethylacetophenone in a weight ratio of 2:1.

[0029] Prepare 1 μg / μL 3-hexanone and 1 μg / μL 3-ethylacetophenone respectively, use liquid paraffin as the dilution solvent, stir until the mixture is uniform, and then dilute with liquid paraffin to prepare a 10 ng / μL attractant solution.

[0030] Comparative Example 3

[0031] The attractant was prepared by diluting 2,2-di(2-tetrahydrofuryl)propane with liquid paraffin to prepare a 10 ng / μL attractant solution.

[0032] A Y-shaped olfactometer was used to measure the tactic response of P. nemoides to attractants. P. nemoides with intact antennae and relatively active behavior were selected for testing. Each sample test consisted of 100 P. nemoides, with males and females tested separately.

[0033] Cut filter paper strips into 5 cm x 1 cm pieces, fold them into a V-shape, and drip the corresponding samples from Examples 1-4 and Comparative Examples 1-3 into the flavor source bottle on one side. Place a filter paper strip with 20 μl of liquid paraffin on the other side as a control. The Y-shaped tube was placed in a dark box at 26°C.

[0034] Ten insects were tested each time, and they were introduced from the tube mouth. After 5 minutes, the behavioral response of the new pineapple gray mealybugs to the odor source was observed. When the new pineapple gray mealybugs crawled to 1 / 2 of a certain arm and stopped for more than 30 seconds, it was considered to have made a choice for the odor. If it still did not make a choice after 5 minutes, it was considered to have no choice, and the result was recorded. After testing 10 insects, the left and right directions of the "Y"-shaped tube were reversed to eliminate the influence of the environment on the test results; after testing 20 insects, they were cleaned with acetone, and then the inner wall of the olfactometer was wiped with cotton wool soaked in ethanol, rinsed with distilled water, and dried in an oven. The above operation was regarded as one repetition, and each treatment was repeated 5 times, and females and males were tested separately.

[0035] Figure 1 The figures are the attracting effects of Examples 1-4 and Comparative Examples 1-3 on male Pseudomonas aeruginosa. Figure 2 The figure shows the attracting effect of Examples 1-4 and Comparative Examples 1-3 on female P. chinensis. As can be seen from the figure, the attractant of this example has a significant attracting effect on P. chinensis, especially when the concentration is 10 ng / μL, the attracting effect is the best, and the attracting effect on female P. chinensis is even better.

Claims

1. A new pineapple mealybug attractant, characterized in that: The invention is composed of the following components in parts by weight: 5-6 parts of 3-hexanone, 10-12 parts of 3-hexanol, 10-12 parts of 2-hexanol, 2-3 parts of 3-ethylacetophenone, and 2-3 parts of 2,2-di(2-tetrahydrofuryl)propane.

2. The new pineapple mealybug attractant according to claim 1, wherein: The invention is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone, and 2.5 parts of 2,2-di(2-tetrahydrofuryl)propane.

3. The application of the new pineapple gray mealybug attractant according to any one of claims 1 to 2 in preventing and treating the new pineapple gray mealybug.

4. The use according to claim 3, characterized in that: The attractant is mixed with a carrier, or adsorbed in the carrier, or sprayed on weeds in a sisal field.

5. The use according to claim 4, characterized in that: The carrier is one of rubber, polyethylene sustained-release bottle, liquid paraffin and activated carbon.

6. The use according to claim 5, characterized in that: The concentration of the attractant in liquid paraffin is 10-1000 ng / μL.

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