The application of vanillyl butyl ether in topical skin preparations, vanillyl butyl ether cyclodextrin inclusion complex and method for preparing fiber masks
Vanillyl butyl ether nanofiber masks were prepared by cyclodextrin coating and electrospinning, which solved the problem of poor water solubility of vanillyl butyl ether and achieved antioxidant and gentle thermal skin care effects, improving the safety and comfort of the mask.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING TECH & BUSINESS UNIV
- Filing Date
- 2024-09-27
- Publication Date
- 2026-06-02
AI Technical Summary
Vanillyl butyl ether has poor water solubility, a problem that current technology cannot effectively solve, which affects its widespread application in the skin care industry. Existing face masks contain inactive ingredients, which can easily cause skin allergies and accelerate aging.
By encapsulating vanillyl butyl ether with the special structure of cyclodextrin, its solubility in water is improved, and nanofiber masks are prepared by electrospinning, combining antioxidant active ingredients with a gentle warming effect.
The high solubility and antioxidant properties of vanillyl butyl ether in water were achieved, resulting in the preparation of a safe and comfortable nanofiber mask that avoids the use of preservatives and stabilizers and has ideal antioxidant and heat-generating effects.
Smart Images
Figure CN119112683B_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of cosmetic technology, and specifically relates to the application of vanillyl butyl ether in topical skin preparations, vanillyl butyl ether cyclodextrin inclusion complexes, and a method for preparing fibrous facial masks. Background Technology
[0002] The human body generates free radicals during normal metabolism, including from respiration, external pollution, and radiation exposure. The accumulation of these free radicals can cause oxidative stress and various pathological reactions. Appropriate use of antioxidants can effectively inhibit the oxidation reaction of free radicals and alleviate skin aging and other problems. Therefore, antioxidant properties are considered a major research and development direction for cosmetic companies and one of the most important functional demands in the market.
[0003] Vanillyl butyl ether, extracted from rosemary and sage, has been shown to produce a mild and lasting warming sensation upon application to the skin, promoting capillary blood circulation and accelerating the absorption of active ingredients. It is commonly added to cosmetics and personal care products as a warming agent. However, other properties of vanillyl butyl ether have not been reported, and its poor solubility in water limits its widespread application; currently, it is only used in oil-phase systems.
[0004] Facial masks, as a commonly used skincare product, offer rapid hydration and skincare benefits, making them an indispensable part of daily life. However, most commercially available masks are water-based sheet masks, containing various inactive ingredients such as preservatives and thickeners, which can easily cause skin problems such as allergies and accelerated aging. Therefore, people are more inclined to accept novel, green, and safe masks to reduce skin damage, but reports on heat-generating nanofiber masks are still rare.
[0005] Therefore, there is an urgent need in the field to develop the potential properties of vanillyl butyl ether and improve its water solubility in order to expand its application in skin care products or other daily chemical products. Summary of the Invention
[0006] The technical problem this application aims to solve is to develop the potential properties of vanillyl butyl ether, overcome its poor water solubility, lack of modification methods, and the absence of research on the activity of vanillyl butyl ether cyclodextrin inclusion complexes after preparation of masks by electrospinning. This application provides a method for the application of vanillyl butyl ether in topical skin agents, vanillyl butyl ether cyclodextrin inclusion complexes, and the preparation of fiber masks. This application has discovered that vanillyl butyl ether possesses antioxidant activity. Utilizing the special structure of cyclodextrin and encapsulating vanillyl butyl ether with encapsulation technology, high water solubility of vanillyl butyl ether is achieved, improving its application scope. Nanofiber masks were successfully prepared using electrospinning, achieving ideal antioxidant effects and a gentle warming effect.
[0007] This application adopts the following technical solution to solve the above-mentioned technical problems:
[0008] This application provides the use of vanillyl butyl ether in topical skin preparations, whereby vanillyl butyl ether is used as an antioxidant active ingredient in topical skin preparations;
[0009] The amount of vanillyl butyl ether added to the topical skin preparation is 0.01 to 10 wt%.
[0010] In some embodiments, the vanillyl butyl ether is encapsulated with cyclodextrin to obtain a vanillyl butyl ether cyclodextrin inclusion complex, which is used in the topical skin agent.
[0011] In some embodiments, the amount of vanillyl butyl ether added to the topical skin preparation is 0.05 to 6 wt%, for example, 1%.
[0012] In some embodiments, the preparation method of the vanillyl butyl ether cyclodextrin inclusion complex specifically includes the following steps:
[0013] (1) Dissolve vanillyl butyl ether in ethanol to prepare vanillyl butyl ether alcohol solution;
[0014] (2) Dissolve the cyclodextrin in water and stir until completely dissolved to obtain a cyclodextrin aqueous solution;
[0015] (3) Add the vanillyl butyl ether alcohol solution prepared in step (1) to the cyclodextrin aqueous solution prepared in step (2), stir to encapsulate, remove the solvent, and dry.
[0016] In step (1), the concentration of vanillyl butyl ether in the vanillyl butyl ether alcohol solution is 0.008-0.1 g / mL, preferably 0.03-0.05 g / mL.
[0017] In step (1), the ethanol is anhydrous ethanol.
[0018] In step (1), the dissolution is carried out under stirring conditions. The stirring is conventional in the art and is generally carried out under a magnetic stirrer.
[0019] When the magnetic stirrer is used for stirring, the stirring speed is 100-800 rpm, preferably 300-400 rpm.
[0020] When a magnetic stirrer is used for stirring, the stirring time is 60 to 120 minutes, preferably 90 minutes.
[0021] In step (1), the dissolution is carried out at room temperature.
[0022] In step (2), the concentration of cyclodextrin in the cyclodextrin aqueous solution is 0.007-0.5 g / mL, preferably 0.045-0.055 g / mL.
[0023] In step (2), the stirring is the stirring that is conventionally performed in the art, generally under magnetic stirring.
[0024] When a magnetic stirrer is used for stirring, the rotation speed is 100-800 rpm, preferably 300-400 rpm.
[0025] When a magnetic stirrer is used for stirring, the stirring time is 60 to 120 minutes, preferably 90 minutes.
[0026] In step (2), the cyclodextrin includes one of hydroxypropyl-β-cyclodextrin, sulfobutyl-β-cyclodextrin, and methyl-β-cyclodextrin, preferably hydroxypropyl-β-cyclodextrin.
[0027] In step (3), after the vanillyl butyl ether alcohol solution is mixed with the cyclodextrin aqueous solution, the molar ratio of vanillyl butyl ether to cyclodextrin is 1:(1-2).
[0028] In step (3), the dripping rate is 1 to 5 mL / min, preferably 3 mL / min.
[0029] In step (3), the stirring is the stirring that is conventionally performed in the art, generally under magnetic stirring.
[0030] When a magnetic stirrer is used for stirring, the stirring speed is 100-800 rpm, preferably 300-400 rpm.
[0031] When a magnetic stirrer is used for stirring, the stirring time is 6 to 24 hours, preferably 12 hours.
[0032] In step (3), the inclusion method is as follows: the vanillyl butyl ether is included in the cavity structure inside the cyclodextrin to carry out the inclusion reaction.
[0033] In step (3), the method for removing the solvent includes vacuum evaporation.
[0034] When using the aforementioned reduced-pressure evaporation method, it can be carried out in a rotary evaporator as is customary in the art.
[0035] The temperature of the vacuum evaporation can be 35-45℃.
[0036] The time for vacuum evaporation can be 20 to 45 minutes.
[0037] In some embodiments, the drying in step (3) is conventional in the art, generally freeze drying.
[0038] When the freeze-drying is performed, the process also includes pre-freezing the sample. Preferably, the pre-freezing temperature is -30 to -10°C, and more preferably -20°C.
[0039] When a pre-freezing operation is used, the pre-freezing time is 2 to 24 hours, preferably 12 hours.
[0040] When the freeze-drying is performed, the freeze-drying temperature is -90 to -70°C, preferably -80°C.
[0041] When the freeze-drying process is performed, the freeze-drying time is 24 to 72 hours, preferably 48 hours.
[0042] In some embodiments, the vanillyl butyl ether is used as an antioxidant active ingredient in the topical skin agent that has ABTS free radical scavenging activity and / or DPPH free radical scavenging activity.
[0043] The vanillyl butyl ether cyclodextrin inclusion complex is an antioxidant active ingredient in the topical skin agent that has ABTS free radical scavenging activity and / or DPPH free radical scavenging activity.
[0044] The vanillyl butyl ether or the vanillyl butyl ether cyclodextrin inclusion complex is a pyrogenic ingredient in the topical skin agent. The pyrogenic ingredient has a pyrogenic effect in 1 to 15 minutes and lasts for 15 to 30 minutes.
[0045] The topical skin agent includes a face mask, lotion, or cream, preferably a face mask, such as a nanofiber face mask.
[0046] In some embodiments, the nanofiber mask is prepared by electrospinning, and the electrospinning preparation method specifically includes the following steps:
[0047] I. The vanillyl butyl ether cyclodextrin inclusion complex and the active ingredient are compounded in a polymer spinning aid solution, stirred, and ultrasonically treated to obtain a spinning solution;
[0048] II. Fix the spinning solution and spray it into the receiving device.
[0049] In some embodiments, in step (I), the mass concentration of the vanillyl butyl ether cyclodextrin inclusion complex is 0.1–20 wt%; preferably 2–5 wt%.
[0050] In step (I), the polymeric spinning aid solution may generally include at least one of polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, pullulan, pectin, gelatin, gum arabic, and sodium alginate.
[0051] In step (I), the concentration of the polymer spinning aid solution is 5-50 wt%, preferably 8-20 wt%.
[0052] In step (I), the active ingredients may also include active ingredients commonly used in the art, which may generally include at least one of moisturizing active ingredients, whitening active ingredients, anti-inflammatory active ingredients, anti-allergic active ingredients and antioxidant active ingredients.
[0053] The moisturizing active ingredient includes sodium hyaluronate. When sodium hyaluronate is added, its concentration is 0.5–10 wt%, preferably 1–4 wt%.
[0054] In some embodiments, in step (I), the stirring continues until the material is completely dissolved. The stirring time is 2–24 hours, preferably 12 hours.
[0055] In some embodiments, in step (I), the ultrasonic treatment is ultrasonic degassing treatment.
[0056] When the ultrasonic degassing treatment is used, the ultrasonic time is 10-20 minutes, preferably 15 minutes.
[0057] When the ultrasonic degassing treatment is used, the frequency of the ultrasound is 150-300W, preferably 200-250W.
[0058] In some embodiments, the fixing in step (II) is conventional in the art and is generally performed on an electrospinning apparatus.
[0059] When the fixing is performed on the electrospinning device, the fixing voltage is 9-20 kV, preferably 12-16 kV, and more preferably 14-15 kV.
[0060] In some embodiments, in step (II), the spraying is conventional injection spraying in the art, which generally involves using a syringe to draw up the spinning solution and spraying it under the nonwoven fabric film.
[0061] Preferably, the spraying flow rate is 0.2 to 0.6 mL / h, more preferably 0.3 to 0.4 mL / h.
[0062] Preferably, the spraying time is 1 to 20 hours, more preferably 4 to 8 hours.
[0063] Preferably, the receiving distance for the spraying is 5 to 30 cm, more preferably 15 to 20 cm, for example 15 cm.
[0064] This application also provides a fiber mask containing a vanillyl butyl ether cyclodextrin inclusion complex, which is prepared by electrospinning as described above.
[0065] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of this application.
[0066] All reagents and raw materials used in this application are commercially available.
[0067] The positive and progressive effects of this application are as follows:
[0068] This application has discovered that vanillyl butyl ether possesses potential antioxidant activity due to the presence of a phenolic hydroxyl group in its structure. Furthermore, this application utilizes the unique structure of cyclodextrin to encapsulate vanillyl butyl ether using the preparation method described herein, achieving high solubility in water. When applied to a base solution, it can be combined with other active ingredients and film-forming agents to exert a synergistic effect. Simultaneously, this application has successfully prepared nanofiber masks using high-voltage electrospinning. Free radical scavenging experiments and human body thermal sensation experiments have confirmed its ideal antioxidant and heat-generating effects, without the need for preservatives or stabilizers, ensuring high safety and a comfortable user experience. Attached Figure Description
[0069] This application can be better understood by referring to the description given below in conjunction with the accompanying drawings. These drawings, together with the detailed description below, are included in and form part of this specification, and are used to further illustrate preferred embodiments of the application and explain the principles and advantages of the application. Wherein:
[0070] Figure 1 The graph shows the scavenging effect of different concentrations of vanillyl butyl ether ethanol solutions on DPPH free radicals.
[0071] Figure 2 Scanning electron microscope image of the inclusion complex of hydroxypropyl-β-cyclodextrin and vanillyl butyl ether cyclodextrin obtained in Example 1 of this application;
[0072] Figure 3 Thermogravimetric analysis curves of vanillyl butyl ether and the vanillyl butyl ether cyclodextrin inclusion complex obtained in Example 1 of this application;
[0073] Figure 4 This is a scanning electron microscope image of the nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate obtained in Example 1 of this application;
[0074] Figure 5The image shows the sustained-release chloroprene (CDR) of the vanillyl butyl ether cyclodextrin inclusion complex and the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate nanofiber mask obtained in Example 1 of this application.
[0075] Figure 6 This is a comparison chart showing the scavenging effects of vanillyl butyl ether cyclodextrin inclusion complexes at different concentrations on ABTS free radicals obtained in Examples 1-3 and Comparative Examples 1-3 of this application.
[0076] Figure 7 This is a comparison chart showing the scavenging effects of vanillyl butyl ether cyclodextrin inclusion complexes at different concentrations on DPPH free radicals obtained in Examples 1-3 and Comparative Examples 1-3 of this application.
[0077] Figure 8 This is a comparison of the scavenging effects of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate nanofiber masks obtained in Examples 1-3 and Comparative Examples 1-3 of this application on ABTS free radicals. Detailed Implementation
[0078] The present application is further illustrated below by way of embodiments, but these embodiments are not intended to limit the scope of the present application. Experimental methods not specifically described in the following embodiments are performed according to conventional methods and conditions, or as selected in accordance with the product instructions.
[0079] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.
[0080] The vanillyl butyl ether used in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.
[0081] The ethanol used in the following examples and comparative examples was purchased from Shanghai Titan Technology Co., Ltd.
[0082] The hydroxypropyl-β-cyclodextrin used in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.
[0083] The β-cyclodextrin used in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.
[0084] Sodium hyaluronate used in the following examples and comparative examples was purchased from Bloomage Biotechnology Co., Ltd.
[0085] Pullulose used in the following examples and comparative examples was purchased from Zhongke Hongji Biotechnology Co., Ltd.
[0086] Example 1
[0087] A method for preparing a vanillyl butyl ether cyclodextrin inclusion complex includes the following steps:
[0088] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether alcohol solution with a concentration of 0.05g / mL.
[0089] (2) Weigh 3.67g of hydroxypropyl-β-cyclodextrin and dissolve it in 70mL of deionized water. Stir magnetically until completely dissolved to obtain an aqueous solution of hydroxypropyl-β-cyclodextrin with a concentration of 0.0524g / mL.
[0090] (3) 10 mL of vanillyl butyl ether ethanol solution was added dropwise to 70 mL of cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 12 h at a speed of 300 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and the solvent was removed by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.
[0091] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0092] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared above and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0093] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, set the voltage to 15kV, the receiving distance to 15cm, the liquid flow rate to 0.4mL / h, and the receiving screen to be a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0094] Example 2
[0095] A method for preparing a vanillyl butyl ether cyclodextrin inclusion complex includes the following steps:
[0096] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether ethanol solution with a concentration of 0.05g / mL.
[0097] (2) Weigh 7.33g of hydroxypropyl-β-cyclodextrin and dissolve it in 140mL of deionized water. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain an aqueous solution of hydroxypropyl-β-cyclodextrin with a concentration of 0.0524g / mL.
[0098] (3) 10 mL of vanillyl butyl ether ethanol solution was added dropwise to 140 mL of cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 12 h at a speed of 300 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and the solvent was removed by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.
[0099] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0100] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared above and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0101] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, set the voltage to 15kV, the receiving distance to 15cm, the liquid flow rate to 0.4mL / h, and the receiving screen to be a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0102] Example 3
[0103] A method for preparing a vanillyl butyl ether cyclodextrin inclusion complex includes the following steps:
[0104] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether alcohol solution with a concentration of 0.05g / mL.
[0105] (2) Weigh 3.67g of hydroxypropyl-β-cyclodextrin and dissolve it in 70mL of deionized water. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain an aqueous solution of hydroxypropyl-β-cyclodextrin with a concentration of 0.0524g / mL.
[0106] (3) 50 mL of vanillyl butyl ether ethanol solution was added dropwise to 100 mL of cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 8 h at a speed of 300 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and the solvent was removed by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 40 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.
[0107] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0108] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared above and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0109] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, set the voltage to 15kV, the receiving distance to 15cm, the liquid flow rate to 0.4mL / h, and the receiving screen to be a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0110] Comparative Example 1
[0111] The only difference from Example 1 is that the reagent used is β-cyclodextrin, and the specific operation is as follows:
[0112] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether alcohol solution with a concentration of 0.05g / mL.
[0113] (2) Weigh 2.7g of β-cyclodextrin and dissolve it in 70mL of deionized water. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a β-cyclodextrin aqueous solution with a concentration of 0.0386g / mL.
[0114] (3) 10 mL of vanillyl butyl ether ethanol solution was added dropwise to 70 mL of β-cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 12 h at a speed of 500 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and the solvent was removed by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-β-cyclodextrin inclusion complex.
[0115] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0116] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of vanillyl butyl ether-β-cyclodextrin inclusion complex and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0117] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, fix the voltage at 15kV, the receiving distance at 15cm, the liquid flow rate at 0.4mL / h, and the receiving screen at a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0118] Comparative Example 2
[0119] Compared with Example 1, the only difference is that the molar ratio of vanillyl butyl ether to hydroxypropyl-β-cyclodextrin is 1:4, and the specific operation is as follows:
[0120] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether alcohol solution with a concentration of 0.05g / mL.
[0121] (2) Weigh 14.68g of hydroxypropyl-β-cyclodextrin and dissolve it in 280mL of deionized water. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain an aqueous solution of hydroxypropyl-β-cyclodextrin with a concentration of 0.0524g / mL.
[0122] (3) 10 mL of vanillyl butyl ether ethanol solution was added dropwise to 280 mL of cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 12 h at a speed of 300 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and the solvent was removed by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.
[0123] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0124] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared above and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0125] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, set the voltage to 15kV, the receiving distance to 15cm, the liquid flow rate to 0.4mL / h, and the receiving screen to be a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0126] Comparative Example 3
[0127] Compared with Example 1, the difference lies in the operation in step (3), which is as follows:
[0128] (1) Weigh 0.5g of vanillyl butyl ether and dissolve it in 10mL of anhydrous ethanol. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain a vanillyl butyl ether alcohol solution with a concentration of 0.05g / mL.
[0129] (2) Weigh 3.67g of hydroxypropyl-β-cyclodextrin and dissolve it in 70mL of deionized water. Stir the solution with magnetic force at room temperature until it is completely dissolved to obtain an aqueous solution of hydroxypropyl-β-cyclodextrin with a concentration of 0.0524g / mL.
[0130] (3) 10 mL of vanillyl butyl ether ethanol solution was added dropwise to 70 mL of cyclodextrin aqueous solution at a rate of 3 mL / min. The mixture was magnetically stirred for 1 h at a speed of 300 rpm to carry out the inclusion reaction and obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator to remove the solvent by vacuum evaporation. The heating temperature of the rotary evaporator was 40 °C and the vacuum evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20 °C for 12 h and then freeze-dried at -80 °C for 48 h to obtain a white loose inclusion complex solid, namely vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.
[0131] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate includes the following steps:
[0132] (I) Add 5g pullulan to 45g of water and stir at room temperature for 4 hours until the pullulan is completely dissolved. Stop stirring and degas the solution by sonication for 15 minutes to obtain a pullulan solution (with a pullulan concentration of 10wt%). Weigh 0.5g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared above and 0.5g of sodium hyaluronate and add them to the pullulan solution. Stir for 4 hours until both are completely dissolved. Stop stirring and then degas the solution by sonication for 15 minutes to obtain a spinning solution.
[0133] (II) Use a 5mL syringe to draw the spinning solution from step (I), fix it on the electrospinning device, set the voltage to 15kV, the receiving distance to 15cm, the liquid flow rate to 0.4mL / h, and the receiving screen to be a non-woven mask cloth. Spray for 5h. After spraying, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate is obtained.
[0134] Example 1
[0135] Determination of DPPH free radical scavenging effect. DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is a stable organic free radical with a maximum absorption peak at 517 nm in the visible light region. A higher DPPH free radical scavenging rate indicates a more ideal antioxidant effect. Weigh 20 mg of DPPH into a 250 mL volumetric flask and dilute to volume with anhydrous ethanol to prepare a 0.2 mmol / L DPPH ethanol solution. Dissolve vanillyl butyl ether in ethanol to prepare solutions with concentrations of 0.001 mg / mL, 0.005 mg / mL, 0.01 mg / mL, 0.05 mg / mL, 0.1 mg / mL, 0.5 mg / mL, 1 mg / mL, 5 mg / mL, and 10 mg / mL as test solutions. Mix 100 μL of the test solution with an equal volume of DPPH ethanol solution to prepare the sample group. After gentle shaking, incubate in the dark for 30 min and measure the absorbance at 517 nm. Simultaneously, the DPPH solution and an equal volume of anhydrous ethanol mixture were used as a blank group, and the test solution and an equal volume of anhydrous ethanol mixture were used as a solvent baseline group.
[0136] DPPH free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%
[0137] Table 1
[0138]
[0139]
[0140] Figure 1 Table 1 shows that vanillyl butyl ether has excellent DPPH free radical scavenging rate, indicating that vanillyl butyl ether has good antioxidant effect, and the DPPH free radical scavenging rate is concentration-dependent in the range of 0.001 mg / mL to 10 mg / mL.
[0141] Example 2
[0142] The microstructure of the vanillyl butyl ether cyclodextrin inclusion complex prepared in Example 1 was observed using a ZEISS GeminiSEM 300 scanning electron microscope. The test voltage was 5 kV. See [link to relevant documentation]. Figure 2 .
[0143] Depend on Figure 2It can be seen that hydroxypropyl-β-cyclodextrin has a spherical cavity structure. In the scanning electron microscope (SEM) of the vanillyl butyl ether cyclodextrin inclusion complex prepared in Example 1, the unique spherical cavity structure of hydroxypropyl-β-cyclodextrin disappears, and an irregular blocky structure can be clearly observed. This indicates that vanillyl butyl ether interacts with hydroxypropyl-β-cyclodextrin, causing a change in the morphology of the cyclodextrin molecule. Thermogravimetric analysis of vanillyl butyl ether and the vanillyl butyl ether cyclodextrin inclusion complex is shown below. Figure 3 When vanillyl butyl ether is included with cyclodextrin, the thermal stability of the inclusion complex is significantly better than that of vanillyl butyl ether.
[0144] Example 3
[0145] The microstructure of the nanofiber membrane prepared in Example 1 was observed using a ZEISS GeminiSEM 300 scanning electron microscope at a test voltage of 5 kV. (See attached image.) Figure 4 .
[0146] Depend on Figure 4 It is known that the average diameter of the electrospun mask is 194 nm, and the fibers are uniform, smooth, and free of beads, indicating that the preparation method of the electrospun mask in this application can achieve uniform mixing of vanillyl butyl ether cyclodextrin inclusion complex, sodium hyaluronate, and pullulanose.
[0147] Example 4
[0148] The release rate of vanillyl butyl ether from the vanillyl butyl ether cyclodextrin inclusion complex and the nanofiber mask prepared in Example 1 was tested, and the results are shown in Table 2. Figure 5 .
[0149] The test method includes the following steps: Dissolve 100 mg of the test sample in an Erlenmeyer flask containing 20 ml of water, and place it in a shaker to oscillate at 100 rpm. At regular time intervals, take 4 mL of the release solution, measure the absorbance of the solution at 280 nm, and promptly pour it back into the original Erlenmeyer flask.
[0150] Vanillyl butyl ether release rate = (Actual concentration of vanillyl butyl ether in solution) / (Theoretical concentration of vanillyl butyl ether) × 100%
[0151] Table 2
[0152]
[0153] From Table 2 and Figure 5 This indicates that vanillyl butyl ether has a good release effect in both the inclusion complex and the nanofiber mask of Example 1. It is released rapidly in the first 10 minutes, and then slowly. After 30 minutes, the release amount of vanillyl butyl ether in the inclusion complex can reach more than 80%, and the release amount of vanillyl butyl ether in the nanofiber mask can reach more than 60%.
[0154] Example 5
[0155] The antioxidant capacity of a substance is evaluated by detecting its ability to scavenge ABTS free radicals. A higher ABTS free radical scavenging rate indicates a more ideal antioxidant effect. The ABTS working solution is prepared by incubating equal volumes of ABTS solution (7.4 mmol / L) and K₂S₂O₈ solution (2.6 mmol / L) in the dark for 12 hours. Before measurement, the ABTS working solution is diluted with deionized water to ensure an absorbance at 734 nm between 0.68 and 0.72. The vanillyl butyl ether cyclodextrin inclusion complexes obtained in Examples 1-3 and Comparative Examples 1-3 are dissolved in deionized water to prepare test solutions of different concentrations: 1 mg / mL, 2 mg / mL, 5 mg / mL, and 10 mg / mL. It is worth noting that when the concentration of the vanillyl butyl ether-β-cyclodextrin inclusion complex prepared in Comparative Example 1 was 10 mg / mL or higher, the solution became turbid, indicating that the water solubility of the vanillyl butyl ether-β-cyclodextrin inclusion complex was still low. Therefore, only the solutions at 0.5 mg / mL, 1 mg / mL, and 5 mg / mL were used for free radical scavenging experiments. A 50 μL solution of the inclusion complex was mixed with 150 μL of ABTS diluent to form the sample group. After shaking and mixing, the absorbance of the mixture at 734 nm was measured after 6 min. Deionized water was used to replace the inclusion complex solution as the blank group, and a 50 μL solution of the inclusion complex was mixed with 150 μL of deionized water as the solvent baseline group. The results are shown in Table 3. Figure 6 .
[0156] ABTS free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%.
[0157] Table 3
[0158]
[0159] Table 3 and Figure 6 The results show that the vanillyl butyl ether inclusion complexes prepared in Examples 1-3 have a better ABTS radical scavenging effect than those prepared in Comparative Examples 1-3.
[0160] Example 6
[0161] The vanillyl butyl ether cyclodextrin inclusion complexes prepared in Examples 1-3 and Comparative Examples 1-3 were dissolved in deionized water to prepare test solutions of different concentrations: 1 mg / mL, 2 mg / mL, 5 mg / mL, and 10 mg / mL. 100 μL of the inclusion complex solution was mixed with an equal volume of DPPH ethanol solution to prepare the sample group. After gentle shaking, the mixture was incubated in the dark for 30 min, and the absorbance was measured at 517 nm. Simultaneously, the absorbance of the DPPH solution mixed with an equal volume of deionized water was used as the blank group, and the mixture of the inclusion complex solution and an equal volume of anhydrous ethanol was used as the solvent baseline group.
[0162] DPPH free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%
[0163] The DPPH radical scavenging rates of the vanillyl butyl ether cyclodextrin inclusion complexes prepared in Examples 1-3 and Comparative Examples 1-3 were tested, and the results are shown in Table 4. Figure 7 .
[0164] Table 4
[0165]
[0166] From Table 4 and Figure 7 It can be seen that, at the same concentration, the antioxidant effects of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complexes prepared in Examples 1-3 are better than those in Comparative Examples 1-3, and the antioxidant effect increases with the increase of the concentration of the vanillyl butyl ether cyclodextrin inclusion complex. Moreover, when the concentration of the vanillyl butyl ether cyclodextrin inclusion complex increases to 10 mg / mL, the vanillyl butyl ether cyclodextrin inclusion complex in Comparative Example 1 cannot be completely dissolved, so it cannot be measured.
[0167] Example 7
[0168] The ABTS free radical scavenging ability of the nanofiber masks containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate prepared in Examples 1-3 and Comparative Examples 1-3 was determined. The nanofiber masks were dissolved in deionized water to prepare test solutions of different concentrations, with the mass-to-volume ratio of nanofiber masks in the test solutions being 5 mg / mL, 10 mg / mL, 20 mg / mL, and 30 mg / mL, respectively. Specific experimental procedures are shown in Example 5. The ABTS free radical scavenging rates are shown in Table 5. Figure 8 As shown.
[0169] Table 5
[0170]
[0171] From Table 5 and Figure 8 It can be seen that the nanofiber membranes prepared in Examples 1-3 have a better ABTS free radical scavenging effect than those prepared in Comparative Examples 1-3.
[0172] Example 8
[0173] The experimental group consisted of 12 volunteers aged 18-60 who met the experimental requirements. The nanofiber membrane prepared in Example 1 was cut into 5cm × 5cm pieces, applied to the inner forearm of the subjects, and moistened with a small amount of deionized water. Timing was started from the dissolution of the nanofiber membrane, and the time to the onset of a warming sensation was recorded within 30 minutes, along with any skin irritation reactions (obvious erythema, itching, swelling, stinging, etc.). The test results are shown in Table 6. "-" = no reaction; "+" = reaction.
[0174] Table 6
[0175]
[0176] As shown in Table 6, all volunteers experienced a warming sensation within 15 minutes, indicating a heating effect, consistent with the typical usage time of the mask (15-30 minutes). Among the 12 volunteers, 4 experienced a warming sensation within 1-5 minutes, and 9 experienced a warming sensation within 10 minutes, demonstrating the widespread adaptability of the nanofiber mask prepared in Example 1. Furthermore, no significant irritation reactions such as redness, itching, or swelling were observed on the test skin of any of the volunteers.
[0177] Finally, it should be noted that the terms “comprising,” “including,” or any other variations thereof in this application are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
[0178] Although this application has been disclosed above through the description of specific embodiments, it should be understood that those skilled in the art can devise various modifications, improvements, or equivalents to this application within the spirit and scope of the appended solutions. Such modifications, improvements, or equivalents should also be considered to be included within the scope of protection claimed in this application.
Claims
1. Use of vanillylbutyl ether for the preparation of a skin external agent, characterized in that, Vanillyl butyl ether is used as an antioxidant and pyrogenic ingredient in topical skin preparations. The vanillyl butyl ether is encapsulated with cyclodextrin to obtain a vanillyl butyl ether cyclodextrin inclusion complex, which is used in the topical skin agent; the cyclodextrin is hydroxypropyl-β-cyclodextrin; the amount of vanillyl butyl ether added to the topical skin agent is 0.05~10wt%; the topical skin agent is a nanofiber mask; The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex specifically includes the following steps: (1) Dissolve the vanillyl butyl ether in ethanol to prepare a vanillyl butyl ether alcohol solution; (2) Dissolve the cyclodextrin in water and stir until completely dissolved to obtain a cyclodextrin aqueous solution; (3) The vanillyl butyl ether alcohol solution prepared in step (1) is added dropwise to the cyclodextrin aqueous solution prepared in step (2), stirred to encapsulate, the solvent is removed, and dried; wherein, after the vanillyl butyl ether alcohol solution and the cyclodextrin aqueous solution are mixed, the molar ratio of vanillyl butyl ether to cyclodextrin is 1: (1~2). In step (3), the stirring time is 6 to 24 hours.
2. Use of vanillyl butyl ether according to claim 1 for the manufacture of a skin external preparation, characterized in that, The amount of vanillyl butyl ether added to the topical skin preparation is 0.05~6 wt%.
3. The application of vanillyl butyl ether as described in claim 2 in the preparation of topical skin agents, characterized in that, The amount of vanillyl butyl ether added to the topical skin preparation is 1 wt%.
4. The application of vanillyl butyl ether as described in claim 1 in the preparation of topical skin agents, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies at least one of the following conditions: In step (1), the concentration of vanillyl butyl ether in the vanillyl butyl ether alcohol solution is 0.008~0.1g / mL; In step (1), the ethanol is anhydrous ethanol; In step (1), the stirring speed is 100~800 rpm; In step (1), the stirring time is 60~120 min.
5. The application of vanillyl butyl ether as described in claim 4 in the preparation of topical skin preparations, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies at least one of the following conditions: In step (1), the concentration of vanillyl butyl ether in the vanillyl butyl ether alcohol solution is 0.03~0.05 g / mL; In step (1), the stirring speed is 300~400 rpm; In step (1), the stirring time is 90 minutes.
6. The use of vanillyl butyl ether as described in claim 1 in the preparation of topical skin agents, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies at least one of the following conditions: In step (2), the concentration of cyclodextrin in the cyclodextrin aqueous solution is 0.007~0.5g / mL; In step (2), the stirring speed is 100~800 rpm; In step (2), the stirring time is 60~120 min; In step (3), the dripping rate is 1~5 mL / min; In step (3), the stirring speed is 100~800 rpm.
7. The use of vanillyl butyl ether as described in claim 6 in the preparation of topical skin agents, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies at least one of the following conditions: In step (2), the concentration of cyclodextrin in the cyclodextrin aqueous solution is 0.045~0.055 g / mL; In step (2), the stirring speed is 300~400 rpm; In step (2), the stirring time is 90 minutes; In step (2), the cyclodextrin is hydroxypropyl-β-cyclodextrin; In step (3), the dripping rate is 3 mL / min; In step (3), the stirring speed is 300~400 rpm; In step (3), the stirring time is 12 hours.
8. The use of vanillyl butyl ether as described in any one of claims 1 to 6 in the preparation of topical skin preparations, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies at least one of the following conditions: The solvent removal method includes vacuum evaporation and / or freeze drying. When the vacuum evaporation method is used, the vacuum evaporation temperature is 35~45℃; and the vacuum evaporation time is 20~45min. The drying process is freeze-drying. When freeze-drying is performed, it also includes a pre-freezing operation of the sample. The freeze-drying temperature is -90 to -70°C, and the freeze-drying time is 24 to 72 hours.
9. The use of vanillyl butyl ether as described in claim 8 in the preparation of topical skin agents, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies the following requirements: The pre-freezing temperature is -30 to -10°C; the pre-freezing time is 2 to 24 hours; the freeze-drying temperature is -80°C; and the freeze-drying time is 48 hours.
10. The use of vanillyl butyl ether as described in claim 9 in the preparation of topical skin agents, characterized in that, The preparation method of the vanillyl butyl ether cyclodextrin inclusion complex satisfies the following requirements: The pre-freezing temperature is -20℃; the pre-freezing time is 12 hours.
11. The use of vanillyl butyl ether as described in claim 1 or 2 in the preparation of topical skin preparations, characterized in that, The vanillyl butyl ether is an antioxidant active ingredient in the topical skin agent that has ABTS free radical scavenging activity and / or DPPH free radical scavenging activity.
12. The use of vanillyl butyl ether as described in claim 11 in the preparation of topical skin preparations, characterized in that, The vanillyl butyl ether cyclodextrin inclusion complex serves as an antioxidant active ingredient in the topical skin agent that has ABTS free radical scavenging activity and / or DPPH free radical scavenging activity; And / or, the vanillyl butyl ether or the vanillyl butyl ether cyclodextrin inclusion complex serves as the pyrogenic active ingredient in the topical skin agent.
13. The use of vanillyl butyl ether as described in claim 12 in the preparation of topical skin agents, characterized in that, The heating ingredient produces a heating effect in 1 to 15 minutes, and lasts for 15 to 30 minutes.
14. The use of vanillyl butyl ether as described in claim 11 in the preparation of topical skin preparations, characterized in that, The nanofiber mask is prepared by electrospinning, and the electrospinning preparation method specifically includes the following steps: I. The vanillyl butyl ether cyclodextrin inclusion complex and the active ingredient are compounded in a polymer spinning aid solution, stirred, and ultrasonically treated to obtain a spinning solution; II. Fix the spinning solution and spray it into the receiving device.
15. The use of vanillyl butyl ether as described in claim 14 in the preparation of topical skin preparations, characterized in that, The electrospinning preparation method of the above satisfies at least one of the following conditions: In step (I), the mass concentration of the vanillyl butyl ether cyclodextrin inclusion complex is 0.1~20 wt%; In step (I), the polymeric spinning aid solution includes at least one of polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, pullulan, pectin, gelatin, gum arabic, and sodium alginate; In step (I), the concentration of the polymer spinning aid solution is 5-50 wt%; In step (I), the active ingredient further includes at least one of moisturizing active ingredient, whitening active ingredient, anti-inflammatory active ingredient, anti-allergic active ingredient and antioxidant active ingredient; In step (I), the stirring time is 2~24h; In step (I), the ultrasonic treatment is ultrasonic degassing treatment; In step (II), the fixing is performed on the electrospinning device; In step (II), the spraying is performed by using a syringe to draw up the spinning solution and spraying it under the nonwoven fabric film.
16. The use of vanillyl butyl ether as described in claim 15 in the preparation of topical skin preparations, characterized in that, The electrospinning preparation method of the above satisfies at least one of the following conditions: In step (I), the mass concentration of the vanillyl butyl ether cyclodextrin inclusion complex is 2-5 wt%. In step (I), the concentration of the polymer spinning aid solution is 8~20 wt%; In step (I), the moisturizing active ingredient includes sodium hyaluronate; In step (I), the stirring time is 12 hours; In step (I), when the ultrasonic degassing treatment is used, the ultrasonic time is 10-20 min; the ultrasonic frequency is 150-300 W. In step (II), when the fixing is performed on the electrospinning device, the fixing voltage is 9~20KV; In step (II), the spraying flow rate is 0.2~0.6 mL / h; the spraying time is 4~8 h; and the spraying receiving distance is 5~30 cm.
17. The use of vanillyl butyl ether as described in claim 16 in the preparation of topical skin preparations, characterized in that, The electrospinning preparation method of the above satisfies at least one of the following conditions: In step (I), when the sodium hyaluronate is added, the concentration of the sodium hyaluronate is 0.5~10 wt%; In step (I), when the ultrasonic degassing treatment is used, the ultrasonic time is 15 minutes; the ultrasonic frequency is 200~250W. In step (II), when the fixing is performed on the electrospinning device, the fixing voltage is 12~16KV; In step (II), the spraying flow rate is 0.3~0.4 mL / h; the spraying time is 4~8 h; and the spraying receiving distance is 15~20 cm.
18. The use of vanillyl butyl ether as described in claim 17 in the preparation of topical skin preparations, characterized in that, The electrospinning preparation method of the above satisfies at least one of the following conditions: In step (I), when the sodium hyaluronate is added, the concentration of the sodium hyaluronate is 1~4 wt%; In step (II), when the fixing is performed on the electrospinning device, the fixing voltage is 14~15KV; In step (II), the receiving distance for spraying is 15cm.
19. A nanofiber mask of vanillyl butyl ether cyclodextrin inclusion complex, prepared by the electrospinning method according to any one of claims 14-18.