A traditional Chinese medicine oral care solution, a preparation method and application thereof

Abstract

The application belongs to the technical field of oral care, and particularly relates to a traditional Chinese medicine oral care solution, a preparation method and application thereof. Compared with traditional mouthwash and oral care solution, the traditional Chinese medicine oral care solution provided by the application contains various traditional Chinese medicine extracts which are matched with each other and synergistically enhanced, and has the effects of beneficial antibacterial and antiviral and saliva production. Meanwhile, the borate solution contained in the product plays a role in adjusting the pH value of the oral cavity, and synergistically enhances the traditional Chinese medicine components, so as to assist the traditional Chinese medicine extract in improving the oral microecology and prolonging the protection time of the traditional Chinese medicine extract on the oral cavity, and better achieve the purpose of oral care health, and therefore has good practical popularization and application value.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of oral care, and particularly relates to a traditional Chinese medicine oral care solution and a preparation method and application thereof. BACKGROUND

[0002] The information disclosed in this Background section is for the purpose of increasing the understanding of the background of the application without admitting that such information constitutes prior art.

[0003] With the development of society and the improvement of people's living standards, oral health is receiving more and more attention. Elderly people are more susceptible to oral problems such as periodontal disease and oral mucosa abnormalities due to factors such as body aging, lack of medical treatment, and multiple drug use.

[0004] In addition, it is worth noting that with age, the salivary secretion in the human mouth gradually decreases, and the amount of saliva in the elderly is significantly lower than that of young people. Saliva contains three buffer systems of bicarbonate, phosphate, and protein buffer system, and is an important buffer system in the oral cavity. When the amount of saliva is significantly reduced, the acid-base balance in the oral cavity is disrupted, and harmful bacteria are more likely to breed and reproduce, which will have adverse effects on periodontal health, mucosal health, dental health, and even respiratory health. Therefore, the elderly face more serious oral problems than young people. For intubated elderly people who cannot eat orally, because there is no masticatory stimulation, salivary secretion is even more reduced, and oral problems are more prominent.

[0005] Currently, the main ways to clean the oral cavity are brushing and gargling with mouthwash (oral care solution), but conventional brushing can only remove 40%-50% of plaque on the tooth surface, and has little effect on removing bacteria on the surface of soft tissue in the oral cavity. Mouthwash can reach deep into all parts of the oral cavity, including the gum margin and interdental space that are difficult to reach by toothbrush, and improve gum health, making it a good measure to assist in controlling plaque. Mouthwash is easy to use and convenient to carry, and is the best choice for oral health protection for the elderly.

[0006] Most of the mouthwashes on the market and used in hospitals are prepared by using chemical drugs such as essence, surfactant, fluoride, strontium chloride, alcohol, metronidazole, hydrogen peroxide, etc., which have good antibacterial effect but are easy to produce drug resistance and cause adverse reactions after long-term use. In addition, such chemical mouthwashes can stimulate the oral mucosa, and long-term use will damage the oral mucosa, and even cause oral mucosal peeling in severe cases. The use of metronidazole mouthwash can cause changes in taste, oral mucosa pain, nausea, vomiting and other adverse reactions, and long-term use of hydrogen peroxide can cause dental decalcification and black hairy tongue and other side effects. Overall, long-term use of chemical mouthwash can pose a safety hazard to the body. In addition, the current market does not pay attention to the serious problem of the destruction of the oral acid-base balance of the elderly, and there is no product to meet the demand for adjusting the pH value of the oral cavity of the elderly. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application provides a traditional Chinese medicine oral care solution for the elderly and a preparation method and application thereof. It has been verified through experiments that the product has good antibacterial and antiviral abilities and the ability to adjust the pH value of the oral cavity, can adjust the microecological balance of the oral cavity, relieve inflammation and pain, freshen breath, and improve the overall health status of the oral cavity of the elderly, and has a good experience when used. Based on the above research results, the present application is completed.

[0008] To achieve the above technical purposes, the technical scheme adopted by the present application is as follows:

[0009] In a first aspect of the present application, a traditional Chinese medicine oral care solution is provided, which comprises the following components in mass fraction: traditional Chinese medicine extract 2-5 parts, 3% boric acid solution 2-5 parts; wherein the raw materials of the traditional Chinese medicine extract include honeysuckle 5-20 parts, mint 8-25 parts, reed 8-25 parts, corydalis 1-8 parts, sophora 2-10 parts, and platycodon 1-6 parts.

[0010] In a second aspect of the present application, a preparation method of the above traditional Chinese medicine oral care solution is provided, which comprises mixing the traditional Chinese medicine extract with the 3% boric acid solution.

[0011] The preparation method of the traditional Chinese medicine extract comprises:

[0012] The reed, corydalis, sophora, and platycodon are weighed and mixed according to the above weight ratio, water is added and decocted to boiling, then the honeysuckle and mint are added, and heated, and the obtained filtrate after filtration is the traditional Chinese medicine extract.

[0013] In a third aspect of the present application, the above traditional Chinese medicine oral care solution is applied in any one or more of the following:

[0014] (a) antibacterial or preparation of antibacterial products;

[0015] (b) Antiviral treatment or preparation of antiviral products;

[0016] (c) Adjusting oral pH or preparing products for adjusting oral pH;

[0017] (d) Anti-inflammatory and analgesic, or preparation of anti-inflammatory and analgesic products;

[0018] (e) Freshen breath or products that prepare fresh breath;

[0019] (f) Improve the overall health of the human oral cavity or prepare products that improve the overall health of the human oral cavity.

[0020] One or more of the above technical solutions have the following beneficial technical effects:

[0021] The herbal oral care solution provided by the above technical solution, compared with traditional mouthwash and oral care solutions, contains various herbal extracts that work synergistically to provide beneficial antibacterial, antiviral, and salivation-promoting effects. Simultaneously, the boric acid solution in this product regulates the pH value of the oral cavity, working synergistically with the herbal components to help improve the oral microecology and prolong the protective time of the herbal extracts, thus better achieving the goal of oral health care. Therefore, it has good practical application value. Attached Figure Description

[0022] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0023] Figure 1 The IC50 of the herbal extract and boric acid solution of the present invention 50 Experimental results; where A and B represent the inhibition rates against Escherichia coli; C and D represent the inhibition rates against Staphylococcus aureus; and E and F represent the inhibition rates against Candida albicans.

[0024] Figure 2 The results of antibacterial experiments on the herbal extract, boric acid solution, and mixture of the present invention are shown below; where A represents the inhibition rate against Escherichia coli; B represents the inhibition rate against Staphylococcus aureus; and C represents the inhibition rate against Candida albicans. Detailed Implementation

[0025] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0026] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, steps, operations, elements, components, and / or groups thereof, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and / or groups thereof. It will be understood that the scope of the application is not limited to the specific specific embodiments described below; it is also understood that the terminology used herein is for the purpose of describing specific embodiments only and is not intended to be limiting of the scope of the present application.

[0027] In one exemplary embodiment of the present application, a traditional Chinese medicine oral care solution is provided, which comprises the following components in mass parts: traditional Chinese medicine extract 2-5 parts, 3% boric acid solution 2-5 parts; wherein the raw materials of the traditional Chinese medicine extract include honeysuckle 5-20 parts, mint 8-25 parts, reed 8-25 parts, corydalis 1-8 parts, sophora 2-10 parts, and platycodon 1-6 parts.

[0028] Further, the traditional Chinese medicine oral care solution comprises the following components in mass parts: traditional Chinese medicine extract 2-5 parts, 3% boric acid solution 2-5 parts, and water 0-3 parts.

[0029] In China, natural medicines (traditional Chinese medicines) have a long history of application, small toxic and side effects, and also have bactericidal and anti-inflammatory effects, and are widely used in basic research and clinical practice. Traditional Chinese medicine preparations are widely used in oral diseases, and a large number of studies have shown that traditional Chinese medicine has good efficacy in treating periodontal disease, oral ulcer and other diseases. With people's attention to the safety of mouthwash, the advantages of traditional Chinese medicine mouthwash gradually emerge. Traditional Chinese medicine believes that the gums are passed through by the Yangming meridian, and the teeth are the remainder of the bones, and the oral diseases are mainly related to the stomach and large intestine of the Yangming meridian and the kidney. The etiology and pathogenesis are mainly caused by stomach fire, damp heat in large intestine, and hyperpyrexia of deficiency fire. Therefore, the present application summarizes the traditional Chinese medicines for daily oral care, which are mainly divided into five categories of clearing heat, relieving pain, producing saliva, inhibiting bacteria and freshening breath. In view of the five types of needs, the present application is matched with five traditional Chinese medicinal materials, namely honeysuckle, mint, reed, corydalis, sophora, and platycodon is added to adjust the taste.

[0030] The mint is a labiatae plant mint (Mentha haplocalyx Briq. Mentha haplocalyxThe dried aerial parts of Mentha haplocalyx Briq. The whole plant has a fragrant green smell and is one of the commonly used traditional Chinese medicines in China. It is a pungent and cool diaphoretic antipyretic drug, entering the lung meridian and liver meridian. It dispels wind-heat, clears the head and eyes, relieves sore throat and rash, soothes the liver and promotes qi movement, and is commonly used in the treatment of sores in the mouth, pharynx, throat, gum swelling and pain, etc. Research has shown that the main components of mint are volatile oils, organic acids, flavonoids, etc. As one of the main pharmacodynamic components of mint, volatile oils have biological activities such as anti-inflammatory, analgesic, antibacterial, and anti-tumor; the catechol acid contained in mint is an effective anti-inflammatory agent, which can inhibit 3α-hydroxysteroid dehydrogenase to play an anti-inflammatory role. Mint can also promote the secretion of respiratory mucus, remove the mucus attached to the mucosa, and reduce frothy sputum to increase the effective ventilation volume of the respiratory tract. In addition, mint contains a monoterpene compound, which can reach the lungs through blood circulation, making people feel fresh breath when breathing, and has the effect of relieving the discomfort of gum inflammation and swelling. At the same time, mint has strong biological activity and has been proven to have antibacterial and effective prevention and treatment of oral ulcers and other effects.

[0031] Flos Lonicerae comes from the dried flower buds or flowers with newly opened flowers of Lonicera japonica Thunb. of the family Caprifoliaceae, and has the effects of clearing heat and detoxifying, dispelling wind-heat, and is commonly used in the treatment of heat stroke, prickly heat, boils, and sore throat. Research has shown that Flos Lonicerae can enhance immunity and inhibit the growth of a variety of viruses and bacteria. The functional components of Flos Lonicerae include flavonoids, organic acids, volatile oils, polyphenols, and triterpenoid saponins, which can improve the phagocytosis of white blood cells. Lonicera japonica

[0032] CoryMis yanhusuo Rhizoma Corydalis is the dried tuber of Corydalis yanhusuo W.T.Wang of the family Papaveraceae, with pungent, bitter, and warm properties, belonging to the liver and spleen meridians, and mainly has the functions of promoting blood circulation, promoting qi movement, and relieving pain. Tetrahydropalmatine is the crystal of the extract of Rhizoma Corydalis and is the main active ingredient of Rhizoma Corydalis, with good analgesic effects. Research has shown that Rhizoma Corydalis can help inhibit toothache caused by dental caries, pulpitis, periodontitis, gingivitis, etc.

[0033] Sophora flavescens Radix Sophorae Flavescentis is the dried root of Sophora flavescens of the family Leguminosae, and has the effect of clearing heat and drying dampness, and is one of the commonly used traditional Chinese medicine decoction pieces in clinical practice. The alkaloids and flavonoids in Radix Sophorae Flavescentis are the material basis for its various pharmacological effects such as antibacterial, analgesic, and anti-tumor. As a natural medicine, Radix Sophorae Flavescentis has fewer side effects and is not easy to produce tolerance compared with western medicine preparations such as chlorhexidine and levamisole. It has a good antibacterial effect and also has the effects of preventing, treating or assisting in the treatment of oral mucosal damage, periodontitis, oral cancer and other diseases; the extract of Radix Sophorae Flavescentis can inhibit the growth, adhesion, acid production and sugar production of cariogenic bacteria in the planktonic and biofilm states, and has a good antibacterial effect. Because the matrine bio-gel mainly composed of matrine can help reduce the area of oral ulcers, reduce the degree of oral mucosal damage, and reduce the pain of patients. Sophora flavescens

[0034]

[0034] Rhizoma Phragmitis is fresh or dried rhizome of Phragmites communis Trin. (Poaceae), which is cold in nature and sweet in flavor. It has the functions of clearing heat and purging fire, generating fluid and relieving vomiting, removing restlessness, and diuresis. It has good effects on treating dry mouth, and can treat halitosis caused by dry tongue or gum ulcer. Phragmites communis

[0035] In another specific embodiment of the present application, a preparation method of the traditional Chinese medicine oral care solution is provided, which comprises mixing the traditional Chinese medicine extract and a 3% boric acid solution.

[0036] The preparation method of the traditional Chinese medicine extract comprises:

[0037] Rhizoma Phragmitis, Corydalis, Sophora flavescens, and Platycodon grandiflorum are weighed and mixed according to the above weight ratio, and then water is added for decoction until boiling. Then, honeysuckle and mint are added, and heating is continued. After filtration, the obtained filtrate is the traditional Chinese medicine extract. The viscosity of the traditional Chinese medicine extract is 1.1-1.4 mPa·s.

[0038] Further, in the preparation method of the traditional Chinese medicine extract, the solid-liquid ratio is 1:5-20 (g / mL).

[0039] Further, the water is soaked for 10-60 minutes before decoction.

[0040] Further, after boiling, the water is heated with a low fire for 10-30 minutes, and then the honeysuckle and mint are added. Then, the water is heated with a low fire for another 5-20 minutes.

[0041] In another specific embodiment of the present application, the traditional Chinese medicine oral care solution is applied in any one or more of the following:

[0042] (a) antibacterial or preparation of antibacterial products;

[0043] (b) antiviral or preparation of antiviral products;

[0044] (c) adjusting oral pH or preparation of products for adjusting oral pH;

[0045] (d) anti-inflammatory and analgesic or preparation of anti-inflammatory and analgesic products;

[0046] (e) freshening breath or preparation of products for freshening breath;

[0047] (f) improving the overall health of the human oral cavity or preparation of products for improving the overall health of the human oral cavity.

[0048] In another specific embodiment of the present application, other auxiliary ingredients can be added to the products, so as to obtain different dosage forms, such as water, emulsion, powder, gel, etc., which are not specifically limited herein.

[0049] ​Particularly, the product can be a daily chemical product or a medicine, particularly an oral care product, such as the most common mouthwash, and the use method at least includes the following two: daily use as a mouthwash; for intubated patients, the product can be used with a cotton swab to clean the oral cavity by wiping the gums, teeth, and other parts. It is particularly worth mentioning that the present application has a positive effect on the improvement of the oral environment of the elderly.

[0050] The present application is further explained and described by the following examples, but does not constitute a limitation on the present application. It should be understood that these examples are only used to illustrate the present application and not to limit the scope of the present application.

[0051] Examples 1-7 and Comparative Examples 1-7

[0052] The preparation method of the traditional Chinese medicine oral care solution in each example and comparative example is as follows:

[0053] The rhizoma phragmitis, rhizoma corydalis, sophora flavescens, and platycodon grandiflorum are weighed according to the mass of each example and comparative example and mixed, 500 mL of purified water is added and stirred to soak for 30 minutes, heated to boiling, and gently heated for 20 minutes, then honeysuckle and mint are added and gently heated for 10 minutes, filtered to obtain filtrate A.

[0054] Take 250 mL of filtrate A, mix with 3% boric acid solution in each example and comparative example, and then add purified water to make up to 500 mL, mix and stir evenly to obtain an oral care solution.

[0055] Seven examples and seven comparative examples are set, and the specific formulations are as follows:

[0056]

[0057] Effect verification

[0058] The properties of the oral care solutions with different formulations are compared as follows:

[0059]

[0060] From the experimental results, it can be seen that the color and taste of examples 1, 2, 3, 5, and 6 are in a relatively good state; example 4 is sour because the proportion of 3% boric acid solution is high; example 7 has a strong medicinal taste and bitter taste because the amount of traditional Chinese medicinal materials is large. Comparative example 7 is sour because the proportion of 3% boric acid solution is too high, which is unacceptable in actual use.

[0061] The oral pH regulation ability of the product was determined. Saliva was collected before using the product, 1 hour after using the product, and 3 hours after using the product, and the pH value was determined. The results are as follows:

[0062]

[0063] The results show that the example with a higher amount of boric acid solution was more effective in regulating oral pH than the example with a lower amount. Adding 200 mL of 3% boric acid solution effectively regulated oral pH, maintaining it within a reasonable range (6.6-7.1) even after 3 hours of use. However, excessively high amounts of 3% boric acid solution resulted in an overly acidic overall taste (Comparative Example 7), making it unsuitable for practical use. This product, by adding boric acid solution, regulates oral pH within a reasonable range, preventing the excessive growth of harmful bacteria and exhibiting a certain antibacterial effect by controlling the bacterial environment.

[0064] An antibacterial test was conducted using a prepared oral care solution to examine whether the sample had antibacterial activity. The antibacterial effect was tested according to QBT 2738-2012, "Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products." Under conditions of 20℃-21℃, the oral care solution was applied for 2 minutes. The test bacteria were Escherichia coli, Staphylococcus aureus, and Candida albicans. An inhibition rate ≥50% was considered to have an antibacterial effect, and the test was repeated three times. An inhibition rate ≥90% was considered to have a strong antibacterial effect, and the test was repeated three times. The results are as follows:

[0065]

[0066] The experimental results show that Example 7 has a strong antibacterial effect against Escherichia coli, Staphylococcus aureus, and Candida albicans. This is related to the large dosage and good antibacterial effect of the Chinese medicinal materials used in Example 7. Examples 1-6 also have antibacterial effects against Escherichia coli, Staphylococcus aureus, and Candida albicans. Among them, Examples 3 and 4 show more outstanding effects. The proportion of Chinese medicinal materials in these two examples is appropriate, resulting in a better antibacterial effect. At the same time, the appropriate amount of 3% boric acid solution can effectively regulate the pH value of the oral cavity and inhibit the growth and reproduction of harmful bacteria. It works synergistically with the Chinese medicinal materials to exert a good antibacterial effect from the perspectives of controlling environmental conditions and direct antibacterial action.

[0067] To demonstrate the synergistic effect of traditional Chinese medicine (TCM) liquid and boric acid solution on antibacterial function, the following experiment was conducted. First, different volumes of TCM liquid (the herbal ratio in Example 3) and 3% boric acid solution were taken and diluted to 500 mL for antibacterial testing to determine the IC50 of the TCM liquid and boric acid solution. 50 Value. Experimental results are as follows: Figure 1 As shown, for E. coli, the IC of the traditional Chinese medicine liquid... 50 The value was 253.82 mL, and the IC50 of the boric acid solution was... 50 The value was 4.95%; for Staphylococcus aureus, the IC50 of the traditional Chinese medicine solution was [value missing]. 50 The value was 204.86 mL, and the IC50 of the boric acid solution was... 50 The value was 6.11%; for Candida albicans, the IC50 of the traditional Chinese medicine solution was...50 The IC value of the borate solution is 232.13 mL, and the IC value of the borate solution is 4.08%. 50

[0068] The combination of the traditional Chinese medicine liquid and the borate solution is used as the experimental object to detect the inhibition ability on the three bacteria, and the specific concentration of each experimental object is set as follows:

[0069]

[0070] The concentration gradient setting principle is that the concentration of the present application is based on the IC value, and 1 / 4 IC, 1 / 2 IC, and IC are used as the concentration gradient. The bacteriostatic rate under each concentration is shown in Table 1. 50 50 50 50 Figure 2

[0071] According to the inhibition rate in Table 1, the combined inhibition index of the traditional Chinese medicine liquid and the borate solution at different concentration ratios is calculated. According to the data results, the effect of the traditional Chinese medicine liquid and the borate solution at each concentration ratio is synergistic. Figure 2

[0072]

[0073] Note: The combined index is CI. When CI value < 0.9, it belongs to synergistic effect; when 0.9 < CI value < 1.1, it belongs to additive effect; and when CI value > 1.1, it belongs to antagonistic effect.

[0074] Based on the above experiments, four embodiments with good traditional Chinese medicine ratio and borate solution ratio and four representative comparative examples are selected for human experiments. After using the oral care liquid for ten days, the users are surveyed for the use effect, and the results are as follows:

[0075]

[0076] According to the experimental results, the addition amount of the traditional Chinese medicine materials for clearing heat and detoxifying, relieving inflammation and pain in the oral care liquid has a significant effect on gingival swelling and pain; the addition amount of the traditional Chinese medicine materials for freshening breath has a slightly weaker effect on the degree of breath improvement; the addition of the traditional Chinese medicine materials for inhibiting bacteria and regulating the oral microecological environment significantly improves the severity of dental plaque; and the addition of 3% borate solution has a good synergistic effect on the efficacy of the traditional Chinese medicine materials. Overall, the oral care liquid of the present application has a positive effect on the improvement of the oral environment of the elderly in a relatively short period of time.

[0077] ​​​​​​​The above examples are only for illustrating the technical concept and characteristics of the present application, and the purpose is to enable those skilled in the art to understand the content of the present application and to implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made according to the spirit and essence of the present application shall be covered within the protection scope of the present application.

Claims

1. A traditional Chinese medicine oral care solution, comprising the following components in mass parts: traditional Chinese medicine extract 2-5 parts, 3% boric acid solution 2-5 parts; wherein, The raw materials of the traditional Chinese medicine extract include honeysuckle 5-20 parts, mint 8-25 parts, reed 8-25 parts, corydalis 1-8 parts, sophora 2-10 parts, and platycodon 1-6 parts.

2. The traditional Chinese medicine oral care liquid of claim 1, wherein, The traditional Chinese medicine oral care solution comprises the following components in mass fraction: traditional Chinese medicine extract 2-5 parts, 3% boric acid solution 2-5 parts, and water 0-3 parts.

3. The method for preparing the traditional Chinese medicine oral care solution according to any one of claims 1-2, characterized in that, The preparation method comprises mixing the traditional Chinese medicine extract and the 3% boric acid solution.

4. The production method according to claim 3, wherein The preparation method of the traditional Chinese medicine extract comprises: The reed, corydalis, sophora, and platycodon are weighed and mixed according to the above weight ratio, water is added and boiled, then the honeysuckle and mint are added, and heated, and the obtained filtrate after filtration is the traditional Chinese medicine extract.

5. The production method according to claim 3, wherein In the preparation method of the traditional Chinese medicine extract, the solid-liquid ratio is 1:5-20 (g / mL).

6. The production method according to claim 3, wherein The water is soaked for 10-60 minutes before boiling.

7. The production method according to claim 3, wherein After boiling, the water is heated with a low fire for 10-30 minutes, then the honeysuckle and mint are added, and heated with a low fire for 5-20 minutes.

8. The use of the traditional Chinese medicine oral care solution of claim 1 or 2 in the preparation of any one or more of the following: (a) an antibacterial product; (b) a product for adjusting oral pH; (c) an anti-inflammatory and analgesic product; (d) a product for freshening breath; (e) a product for improving dental plaque.

9. The use according to claim 8, wherein the compound is ###00003### or a pharmaceutically acceptable salt thereof. The product is an aqueous solution, an emulsion, a powder, or a gel.

10. The use according to claim 8, characterized in that, The product is applied to the elderly.

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