Construction method of out-of-focus type myopic animal model and myopic animal model diagnosis device and equipment

Abstract

The invention provides a construction method of an out-of-focus type myopic animal model and a myopic animal model diagnosis device and equipment, and belongs to the technical field of biomedicine. According to the method, a myopic guinea pig model is manufactured by adopting a monocular hyperopia optical defocus method, a Photorefrator small animal infrared optometry unit is applied to the measurement of the eye refraction degree of the out-of-focus myopic model guinea pig, and the development degree of the out-of-focus myopic model guinea pig is quantitatively and objectively judged according to the absolute value of a relative myopic refraction value. An ultrasonic measuring instrument is adopted in parallel to measure the eye axis length of a model animal to assist in judging the development of the model, and the out-of-focus myopia animal model is successfully constructed. The animal model can be applied to pharmacodynamic evaluation of non-clinical ophthalmic drugs, provides more reliable drug effectiveness data support, and lays a foundation for new drug research application and clinical application of new drugs.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical technology, and in particular relates to a method for constructing a defocused myopia animal model and a myopia animal model diagnostic device and equipment. Background Art

[0002] Myopia is a common disease among children, but in many countries, the penetration rate of vision screening for infants and preschoolers is also very low, resulting in a high incidence of lifelong eye diseases. Once myopia occurs, reversible treatment is difficult, so the development of myopia drugs has attracted more and more attention. At present, in order to develop effective myopia treatment drugs, myopia animal models are often used for drug screening, and judging whether the drug is effective in myopia animal models or using drugs at a certain stage of model development is the key content of preclinical test sample pharmacodynamics research. Therefore, it is very necessary to recommend a complete and accurate method for establishing myopia animal models.

[0003] Guinea pigs are often selected to construct animal models of myopia because they can highly simulate the characteristics and molecular basis of the etiology, pathology and prognosis of human diseases. However, the current methods for constructing animal models of myopia are often based on the formation of myopia by inducing diet. For example, the patent with publication number CN 115885920A discloses feeding guinea pigs with high-sugar feed or high-sugar and high-fat feed to increase their blood sugar levels, thereby inducing changes in the local structure and physiological state of the eyeball and forming simple myopia. Although this method can successfully obtain a myopia model, it is difficult to predict whether its pathological mechanism is different from myopia caused by improper use of the eyes, which in turn affects the accurate evaluation of the efficacy of the tested drugs. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide a method for constructing a defocus-type myopia animal model, inducing myopia through the hyperopic optical defocus method, and the stability evaluation of the animal model is conducive to obtaining an animal model close to human natural myopia, greatly improving the accuracy of myopia treatment drug evaluation.

[0005] The present invention provides a method for constructing a defocus myopia animal model, comprising the following steps:

[0006] A myopia guinea pig model is constructed using guinea pigs with healthy eyes, with one eye of the guinea pig being the modeling eye and the other being the control eye;

[0007] The modeling eye is worn with a -8D to -12D concave lens spectacle cover for modeling, the refractive power and axial length of both eyes of the guinea pig are regularly tested on a weekly basis, and the relative myopia refractive value and relative axial length difference formed between the modeling eye and the control eye are calculated;

[0008] When compared with before modeling, the relative myopia refractive value of the guinea pig after modeling was significantly reduced, and the absolute value of the relative myopia refractive value was significantly increased, and / or the relative axial length difference of the guinea pig after modeling was significantly increased, indicating that the defocus myopia animal model was successfully constructed.

[0009] Preferably, the myopia degree of the concave lens includes -10D.

[0010] Preferably, the modeling period is 2 to 4 weeks.

[0011] Preferably, the relative myopia refractive value is calculated according to formula I, and the unit is D;

[0012] Relative myopia refraction value = model eye refraction value - control eye refraction value formula I;

[0013] The relative axial length difference is calculated according to Formula II, in mm;

[0014] Relative axial length difference = axial length of model eye - axial length of control eye (Formula II).

[0015] Preferably, the screening criteria for guinea pigs with healthy eyes include selecting guinea pigs with refractive values ​​within the range of 1.0 to 8.0 D and with binocular refractive difference not exceeding 2.0 D and excluding guinea pigs with ophthalmic diseases.

[0016] The present invention provides application of the defocus type myopia animal model obtained by the construction method in myopia treatment drug screening and / or drug efficacy evaluation.

[0017] Preferably, the myopia treatment drug includes 0.1% atropine eye drops.

[0018] The present invention provides a myopia animal model diagnostic device, comprising: a sample information acquisition module, connected to a central analysis module, for collecting information data of animals, wherein the information data includes the number, age and symptom information of the animals;

[0019] A data acquisition module, connected to the central analysis module, for collecting myopia diagnostic index data of the animal through medical equipment, wherein the myopia diagnostic index includes a refractive value and / or an axial length of the eye;

[0020] A central analysis module, connected to the sample information acquisition module, the data acquisition module and the diagnosis module, for analyzing the statistical differences in relative myopia refractive values ​​and / or relative axial length differences before and after animal modeling;

[0021] A diagnosis module, connected to the central analysis module and the display module, is used to determine whether the myopia model of the animal has been successfully established;

[0022] The display module is connected to the central analysis module and the diagnosis module, and is used to display the collected animal information data, myopia diagnosis index data and diagnosis results.

[0023] Preferably, the medical equipment includes a Photorefractor small animal infrared ophthalmometer and / or an ophthalmic ultrasonic measuring instrument.

[0024] The invention provides a myopia animal model diagnostic device equipped with the device.

[0025] The invention provides a method for constructing a defocus myopia animal model, comprising the following steps: constructing a myopia guinea pig model with healthy eyes, taking one eye of the guinea pig as a modeling eye and the other as a control eye; wearing a spectacle cover with a -8D to -12D concave lens on the modeling eye for modeling, regularly detecting the diopter and axial length of both eyes of the guinea pig in units of weeks, and calculating the relative myopia refraction value and relative axial length difference between the modeling eye and the control eye; when compared with before modeling, the relative myopia refraction value of the guinea pig after modeling is significantly reduced, the absolute value of the relative myopia refraction value is significantly increased, and / or the relative axial length difference of the guinea pig after modeling is significantly increased, indicating that the defocus myopia animal model is successfully constructed. The present invention adopts a monocular hyperopic optical defocus method to prepare a myopic guinea pig model, uses myopic refractive value and axial length as myopia diagnostic indicators, and objectively judges the development degree of the defocus myopia model guinea pig according to the relative myopic refractive value and its absolute value quantitative and the relative axial length difference, thereby realizing the successful construction of a defocus myopia animal model. The defocus myopia animal model constructed by the construction method of the present invention is used to carry out the efficacy evaluation of myopia treatment drugs, which shows that the myopia of the defocus myopia animal model is improved before and after drug administration, which reflects that the defocus myopia animal model constructed by the present invention has good drug evaluation stability, which lays a foundation for the development of clinical myopia treatment drugs. DETAILED DESCRIPTION

[0026] The present invention provides a method for constructing a defocus myopia animal model, comprising the following steps:

[0027] A myopia guinea pig model is constructed using guinea pigs with healthy eyes, with one eye of the guinea pig being the modeling eye and the other being the control eye;

[0028] The modeling eye is worn with a -8D to -12D concave lens spectacle cover for modeling, the refractive power and axial length of both eyes of the guinea pig are regularly tested on a weekly basis, and the relative myopia refractive value and relative axial length difference formed between the modeling eye and the control eye are calculated;

[0029] When compared with before modeling, the relative myopia refractive value of the guinea pig after modeling was significantly reduced, and the absolute value of the relative myopia refractive value was significantly increased, and / or the relative axial length difference of the guinea pig after modeling was significantly increased, indicating that the defocus myopia animal model was successfully constructed.

[0030] The invention uses guinea pigs with healthy eyes as objects to construct a myopia guinea pig model, wherein one eye of the guinea pig is used as a modeling eye and the other eye is used as a control eye; a spectacle cover with a -8D to -12D concave lens is worn on the modeling eye for modeling, the refractive power and axial length of both eyes of the guinea pig are regularly detected in units of weeks, and the relative myopia refractive value and relative axial length difference formed between the modeling eye and the control eye are calculated.

[0031] In the present invention, the guinea pig is preferably a tricolor guinea pig. The screening criteria for the guinea pigs with healthy eyes preferably include selecting guinea pigs with refractive values ​​within the range of 1.0 to 8.0D and binocular refractive difference not exceeding 2.0D and excluding guinea pigs with ophthalmic diseases. The ophthalmic diseases preferably include cataracts, corneal clouding and other ophthalmic diseases that affect vision and optometry accuracy. In an embodiment of the present invention, the right eye of the guinea pig is the model eye, and the left eye of the guinea pig is the control eye. The conversion relationship between the refractive value and the degree of myopia is that the degree of myopia is 50 degrees, and the corresponding refractive value is -0.5D.

[0032] In the present invention, the instrument for measuring the refractive value preferably includes a Photorefractor small animal infrared ophthalmometer (ST-PR.01). The refractive value is preferably measured 3 times, and the average value of each detection value is taken. Before the refractive value is measured, it is preferred to use compound tropicamide eye drops to dilate the pupil.

[0033] In the present invention, the myopia of the concave lens is preferably -10D. The material of the concave lens is preferably polymethyl methacrylate lens. The concave lens is preferably fixed by using a milky white latex balloon as a material to make a glasses cover with a concave lens on one side. The duration of the modeling is preferably 2 to 4 weeks.

[0034] In the present invention, the relative myopia refractive value is preferably calculated according to Formula I, and the unit is D;

[0035] Relative myopia refraction value = model eye refraction value - control eye refraction value formula I;

[0036] The relative axial length difference is preferably calculated according to Formula II, in mm;

[0037] Relative axial length difference = axial length of model eye - axial length of control eye (Formula II).

[0038] In the embodiment of the present invention, the refractive value and axial length of the guinea pig's eyes were measured at 1 week, 2 weeks, 3 weeks and 4 weeks after modeling. The results showed that compared with before modeling, with the extension of modeling time, the relative myopia refractive value of the guinea pig decreased significantly and gradually, and the absolute value of the relative myopia refractive value increased significantly, while the relative axial length difference of the guinea pig increased significantly and gradually, and after the second week of modeling, the relative myopia refractive value tended to be stable, above 2.0D.

[0039] In an embodiment of the present invention, in order to evaluate the stability of the constructed myopia animal model, the drug used to treat juvenile myopia in clinical studies was used. The myopia treatment drug is 0.1% atropine eye drops. The method of the experiment is to administer the myopia treatment drug by eye drops on the day of modeling, twice a day, with 25 μL added each time. The interval between the two administrations is 6 hours. The duration of administration is preferably the same as the duration of modeling. The results showed that compared with the model group, the relative myopia refractive value of the guinea pigs in the positive control group (administered with 0.1% atropine eye drops) was significantly increased 3 to 4 weeks after modeling, and the absolute value of the relative myopia refractive value was significantly reduced. At the same time, the relative axial length difference of the guinea pigs was significantly reduced 3 to 4 weeks after modeling.

[0040] The present invention provides application of the defocus type myopia animal model obtained by the construction method in myopia treatment drug screening and / or drug efficacy evaluation.

[0041] In the present invention, the myopia treatment drug preferably includes 0.1% atropine eye drops. The defocus myopia animal model is used as the drug administration object, and the changes in the relative myopia refractive value and / or relative axial length difference of the animal before and after administration are analyzed to judge the effectiveness of the drug to be tested in treating myopia. The method for judging the effectiveness of the drug is preferably that compared with before administration, the relative myopia refractive value of the guinea pig after administration is significantly increased, and the absolute value of the relative myopia refractive value is significantly reduced, and at the same time, the relative axial length difference of the guinea pig is significantly reduced, indicating that the drug to be tested is effective in treating myopia.

[0042] The present invention provides a myopia animal model diagnostic device, comprising: a sample information acquisition module, connected to a central analysis module, for collecting information data of animals, wherein the information data includes the number, age and symptom information of the animals;

[0043] A data acquisition module, connected to the central analysis module, for collecting myopia diagnostic index data of the animal through medical equipment, wherein the myopia diagnostic index includes a refractive value and / or an axial length of the eye;

[0044] A central analysis module, connected to the sample information acquisition module, the data acquisition module and the diagnosis module, for analyzing the statistical differences in relative myopia refractive values ​​and / or relative axial length differences before and after animal modeling;

[0045] A diagnosis module, connected to the central analysis module and the display module, is used to determine whether the myopia model of the animal has been successfully established;

[0046] The display module is connected to the central analysis module and the diagnosis module, and is used to display the collected animal information data, myopia diagnosis index data and diagnosis results.

[0047] In the present invention, the sample information acquisition module, data acquisition module, central analysis module, diagnosis module and display module are electrically connected. The animals preferably include one or more of mice, rats, guinea pigs and chickens. The medical equipment preferably includes a Photorefractor small animal infrared ophthalmometer and / or an ophthalmic ultrasonic measuring instrument. The calculation method of the relative myopia refractive value and / or the relative axial length difference is the same as that described in the construction method in the above technical solution, and will not be repeated here. The analysis method of the statistical difference is preferably a T test.

[0048] In the present invention, in the diagnostic module, the method for judging whether the myopia model of the animal is successfully constructed is preferably that when compared with before modeling, the relative myopia refractive value of the guinea pig after modeling is significantly reduced, and the absolute value of the relative myopia refractive value is significantly increased, and / or the relative axial length difference of the guinea pig after modeling is significantly increased, it indicates that the defocus type myopia animal model is successfully constructed; otherwise, it indicates that the defocus type myopia animal model is not successfully constructed.

[0049] The invention provides a myopia animal model diagnostic device equipped with the device.

[0050] The present invention also provides the use of refractive value and / or axial length as myopia diagnostic markers in the preparation of products for myopia diagnosis or evaluation of the effectiveness of myopia treatment drugs.

[0051] In the present invention, the product includes a myopia detection reagent or a myopia treatment drug.

[0052] The following is a detailed description of a method for constructing a defocused myopia animal model and a myopia animal model diagnostic device and equipment provided by the present invention in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0053] Example 1

[0054] Construction and verification method of a defocus myopia animal model

[0055] 1) Animal species: Tricolor guinea pigs; Number: 20; Age: 1-2 weeks old; Weight range: 120-180 g; Gender: females: 10; males: 10.

[0056] 2) Testing instruments: Photorefractor small animal infrared ophthalmometer (ST-PR.01); ophthalmic ultrasonic measuring instrument (MD-1000A).

[0057] 3) The main reagents and positive drugs are: modeling lens: PMMA concave lens, mydriatic drug: 0.5% compound tropicamide eye drops; ocular surface anesthetic: proparacaine hydrochloride eye drops; positive drug: 0.1% atropine eye drops;.

[0058] 4) Detection process:

[0059] ①Method for measuring the refractive power of guinea pig eyes using Photorefractor small animal infrared ophthalmometer:

[0060] First, drip compound tropicolide eye drops into the conjunctival sac of both eyes of the animal, once every 5 minutes, 1 drop each time, for a total of 4 times. After the first eye drop, the animal is dark-adapted. After about 40 minutes, the animal is placed on the animal detection platform, and the head is fixed so that the detection eye looks directly at the camera lens, but to avoid oppression of the eye; after fixation, adjust the image brightness to obtain a satisfactory eye image, click the "Settings" button of the instrument to set the mode of the infrared LED light, and then click the "Lower LED row (on / off)" switch to turn on the fourth row of LED lights. If the LED lights do not change after setting, click "UPDATE STATUS" to update the status; finally, perform optometry and click the space bar to start recording. When the setting is turned on to control gaze, the software counts every 10 data collections within the gaze control range and with pupil brightness in the range of 75 to 170 as a set of data. The number of data sets collected is displayed on the software interface. Generally speaking, collecting 10 to 20 sets of data is sufficient.

[0061] ②Method for measuring axial length using ophthalmic ultrasound measuring instrument:

[0062] One drop of proparacaine hydrochloride eye drops was instilled into the conjunctival sac of both eyes of the animal for surface anesthesia. About 1 minute later, the axial length (the distance from the corneal vertex to the anterior surface of the vitreous retina at the posterior pole of the eyeball) was measured using a type A ultrasonic measuring instrument. The measurement was performed 5 times in a row in manual mode with an accuracy of 0.01 mm, and the average value was taken as the final axial length value.

[0063] 5) Main test process:

[0064] ① Screening and grouping before modeling:

[0065] All screened animals were treated with compound tropicamide eye drops to dilate the pupils and test the refraction of both eyes. Each eye of the animal was measured three times according to the above steps, and the average value was taken as the final refraction value, and the result was recorded as the result before modeling (0w). Animals with refraction values ​​within the range of 1.0 to 8.0D and binocular refraction difference not exceeding 2.0D were selected for inclusion in the study. Guinea pigs with ophthalmic diseases such as cataracts and corneal clouding that affect vision and optometry accuracy were excluded. Guinea pigs were randomly divided into two groups according to binocular refraction difference and gender, with 10 animals in each group, half male and half female.

[0066] ② Defocus method to make guinea pig myopia model:

[0067] The myopic guinea pig model was made by the monocular hyperopic optical defocus method. The right eye was modeled and the left eye was used as the control eye. A milky white latex balloon was used as the material to make a spectacle cover with a -10D PMMA concave lens on one side. The lenses were checked and cleaned 1 to 2 times a day. If they were loose, they were put on again. If they were obviously worn, new lenses were replaced. The model was made for 4 weeks. Be careful not to damage or adhere to the eyeball and eyelid.

[0068] ③ Administer eye drops on the first day of modeling, twice a day, with an interval of about 6 hours, for 4 consecutive weeks.

[0069] ④ Before modeling and at the 1st, 2nd, 3rd and 4th week after modeling, the refractive power of the left and right eyes of the animals in the model group and the positive control group was measured with the Photorefractor small animal infrared ophthalmometer. The relative myopia refractive values ​​(D) of the two groups of animals before modeling (recorded as 0w) and at the 1st, 2nd, 3rd and 4th week after modeling (1-4w) were calculated according to formula I and statistically analyzed.

[0070] Relative myopia refraction value (D) = refraction value (D) of the right eye modeling eye - refraction value (D) of the left eye control eye (Formula I).

[0071] ⑤ Before modeling and at the 1st, 2nd, 3rd and 4th week after modeling, the axial length of the left and right eyes of the animals in the model group and the positive control group was measured with an ophthalmic ultrasonic measuring instrument. The relative axial length difference (mm) of the two groups of animals before modeling (recorded as 0w) and at the 1st, 2nd, 3rd and 4th week after modeling (1-4w) was calculated according to formula II and statistically analyzed.

[0072] Relative axial length difference (mm) = axial length of the right model eye - axial length of the left control eye (Formula II).

[0073] 6) Main test results of the present invention:

[0074] ① In this embodiment, the refractive power of the left and right eyes of the animals in the model group and the positive control group was measured at 0 weeks and 1 to 4 weeks, and the relative myopia refractive power was calculated. The results show (Table 1) that compared with before modeling, the relative myopia refractive power of the animals in the model group and the positive control group was significantly reduced 1 to 4 weeks after modeling (P < 0.01), and the absolute value of the relative myopia refractive power was significantly increased (P < 0.01); compared with the model group, the relative myopia refractive power of the animals in the positive control group was significantly increased 3 to 4 weeks after modeling (P < 0.05), and the absolute value of the relative myopia refractive power was significantly reduced (P < 0.05).

[0075] ② In this embodiment, the axial length of the left and right eyes of the animals in the model group and the positive control group was measured at 0w and 1-4w, and the relative axial length difference was calculated. The results show (Table 2) that compared with before modeling, the relative axial length difference of the animals in the model group increased significantly after 1-4 weeks of modeling (P < 0.01); compared with the model group, the relative axial length difference of the animals in the positive control group decreased significantly after 3-4 weeks of modeling (P < 0.05 or P < 0.01). The results suggest that with the extension of the modeling time, the modeling animals show the progression of relative myopia. After the first week of modeling, the relative axial length difference of the model animals gradually increases. After the second week of modeling, the absolute value of the relative myopia of the model animals is above 2.0D, and the defocus myopia guinea pig model tends to be stable; in addition, this model can be used for the effectiveness evaluation of myopia drugs after verification by positive drugs (0.1% atropine eye drops).

[0076] Table 1 Statistical results of the mean refraction of both eyes of animals in the model group and 0.1% atropine eye drops group from 0 to 4 weeks n=10)

[0077]

[0078] Note: Compared with the self-modeling group (0w), * indicates P < 0.05, ** indicates P < 0.01; compared with the model group, # indicates P < 0.05, ## indicates P < 0.01.

[0079] Table 2 Statistical results of the mean axial length of both eyes of animals in the model group and 0.1% atropine eye drops group from 0 to 4 weeks n=10)

[0080]

[0081] Note: Compared with the self-modeling group (0w), * indicates P < 0.05, ** indicates P < 0.01; compared with the model group, # indicates P < 0.05, ## indicates P < 0.01.

[0082] Table 3 Individual results of refraction values ​​of both eyes of animals in the model group and 0.1% atropine eye drops group at 0-4 weeks

[0083]

[0084]

[0085] Table 4 Individual results of axial length of both eyes of animals in the model group and 0.1% atropine eye drops group from 0 to 4 weeks

[0086]

[0087]

[0088]

[0089] The main detection result analysis and judgment of the present invention: the present invention is based on the objective examination of the refractive degree of the eyes of clinical myopia patients by simulated eccentric photographic optometry, and the Photorefractor small animal infrared optometry instrument of Striatech GmbH of Germany is applied to the determination of the refractive degree of the eyes of the defocus myopia model guinea pigs. The development degree of the defocus myopia model guinea pigs is quantitatively and objectively judged according to the absolute value of the relative myopia refractive value, and the ultrasonic measuring instrument is used to measure the axial length of the model animals to assist in judging the development of the model, and the positive drug 0.1% atropine eye drops are used to verify the stability of this model, so that the obtained evaluation method is accurate and reliable.

[0090] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for constructing a defocus myopia animal model, characterized in that: The following steps are involved: A myopia guinea pig model is constructed using guinea pigs with healthy eyes, with one eye of the guinea pig being the modeling eye and the other being the control eye; The modeling eye is worn with a -8D to -12D concave lens spectacle cover for modeling, the refractive power and axial length of both eyes of the guinea pig are regularly tested on a weekly basis, and the relative myopia refractive value and relative axial length difference formed between the modeling eye and the control eye are calculated; When compared with before modeling, the relative myopia refractive value of the guinea pig after modeling was significantly reduced, and the absolute value of the relative myopia refractive value was significantly increased, and / or the relative axial length difference of the guinea pig after modeling was significantly increased, indicating that the defocus myopia animal model was successfully constructed.

2. The construction method according to claim 1, characterized in that: The myopia degree of the concave lens sheet includes -10D.

3. The construction method according to claim 1, characterized in that: The duration of the modeling ranges from 2 to 4 weeks.

4. The construction method according to claim 1, characterized in that: The relative myopia refractive value is calculated according to formula I, and the unit is D; Relative myopia refraction value = model eye refraction value - control eye refraction value formula I; The relative axial length difference is calculated according to Formula II, in mm; Relative axial length difference = axial length of model eye - axial length of control eye (Formula II).

5. The construction method according to any one of claims 1 to 4, characterized in that: The screening criteria for the guinea pigs with healthy eyes include selecting guinea pigs with refractive values ​​within the range of 1.0 to 8.0 D and binocular refractive difference not exceeding 2.0 D and excluding guinea pigs with ophthalmic diseases.

6. Use of the defocus myopia animal model obtained by the construction method according to any one of claims 1 to 5 in the screening of myopia therapeutic drugs and / or the evaluation of drug efficacy.

7. The use according to claim 6, characterized in that: The myopia treatment medicine includes 0.1% atropine eye drops.

8. A myopia animal model diagnostic device, characterized in that: include: A sample information collection module, connected to the central analysis module, for collecting information data of animals, the information data including the number, age and symptom information of the animals; A data acquisition module, connected to the central analysis module, for collecting myopia diagnostic index data of the animal through medical equipment, wherein the myopia diagnostic index includes a refractive value and / or an axial length of the eye; A central analysis module, connected to the sample information acquisition module, the data acquisition module and the diagnosis module, for analyzing the statistical differences in relative myopia refractive values ​​and / or relative axial length differences before and after animal modeling; A diagnosis module, connected to the central analysis module and the display module, is used to determine whether the myopia model of the animal has been successfully established; The display module is connected to the central analysis module and the diagnosis module, and is used to display the collected animal information data, myopia diagnosis index data and diagnosis results.

9. The device according to claim 8, characterized in that: The medical equipment includes a Photorefractor small animal infrared optometry instrument and / or an ophthalmic ultrasonic measuring instrument.

10. A diagnostic device for myopia animal models equipped with the device according to claim 8 or 9.

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