A fluorescence imaging method, device and storage medium based on light source filtering

Abstract

The present application provides a fluorescence imaging method, device and storage medium based on light source filtering, the method comprising: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue, and then the green fluorescence band spectrum generated by the excitation of riboflavin is filtered through a notch filter to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through a bandpass filter to increase the ratio of the green fluorescence band spectrum to the red and blue fluorescence band spectrum, and then passes through a notch filter to filter the green fluorescence band spectrum, the notch filter reduces the ratio of the green fluorescence band spectrum, and the difference between the ratio of the green fluorescence band spectrum increased by the bandpass filter is within a preset range. In solving the problem that the green fluorescence generated by the excitation of riboflavin affects the contrast of PDD fluorescence imaging during PDD fluorescence imaging, the color restoration of traditional white light imaging can also be guaranteed.

Description

Technical Field

[0001] The present invention relates to the field of photodynamic diagnosis, and in particular to a fluorescence imaging method, device and storage medium based on light source filtering. Background Art

[0002] Photodynamic diagnosis (PDD) is a new diagnostic technology that allows photosensitizing substances to accumulate in diseased tissues and irradiates them with excitation light to make them fluoresce. The presence of disease and the diseased tissues can be determined by observing and detecting fluorescence.

[0003] This technology is widely used in the field of medical diagnosis. For example, in the diagnosis of tumors, photosensitizers are selectively enriched in cancer cells; then the cancer tissue is irradiated with excitation light to make the enriched photosensitizers produce fluorescence; by analyzing the intensity and distribution of the fluorescence, the location and condition of the cancer can be determined. For another example, in the process of bladder PDD imaging, blue-violet light is used as the excitation light to irradiate the photosensitizer-aggregated tissue, stimulating red fluorescence and imaging it.

[0004] However, during bladder PDD imaging, the metabolite riboflavin in urine will be excited by the excitation light and emit green fluorescence, thereby interfering with the contrast of PDD fluorescence. The existing method is to change the excitation light band, such as using 500nm as the photosensitizer excitation wavelength to reduce the excitation efficiency of riboflavin. However, changing the excitation wavelength will also cause the fluorescence excitation efficiency of the photosensitizer to decrease, and the fluorescence brightness of PDD imaging will decrease. At the same time, it will cause more loss of visible light imaging band, which is not conducive to traditional white light imaging. Summary of the invention

[0005] The present application provides a fluorescence imaging method, device and storage medium based on light source filtering. By combining a filtering module and a light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging can be ensured.

[0006] In view of this, the present application provides a fluorescence imaging method based on light source filtering, characterized in that the method is applied to a fluorescence imaging device based on light source filtering, in which a notch filter and a bandpass filter are provided, and the device has two modes of photodynamic diagnosis PDD fluorescence mode and photodynamic white light imaging mode. The method includes: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observation tissue, and then filtered through the notch filter to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin, so as to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through the bandpass filter to increase the ratio of the spectrum of the green fluorescence band to the spectrum of the red and blue fluorescence bands, and then passes through the notch filter to filter the spectrum of the green fluorescence band after being reflected by the observation tissue, so that the light after passing through the notch filter is still white light, wherein the difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter is within a preset range.

[0007] In combination with the first aspect, in a possible implementation, the transmittance of the notch filter for a blue band spectrum and a red band spectrum exceeds a first preset transmittance threshold, while the transmittance for a green band spectrum is lower than a second preset transmittance threshold.

[0008] In combination with the first aspect, in a possible implementation manner, the transmittance of the bandpass filter for a green band spectrum exceeds a third preset transmittance threshold, and filters a blue band spectrum and a red band spectrum below a fourth transmittance threshold.

[0009] With reference to the first aspect, in a possible implementation manner, the transmittance of the notch filter and the transmittance of the bandpass filter for light with a wavelength of λ are complementary.

[0010] In combination with the first aspect, in a possible implementation method, the transmittance of the notch filter and the bandpass filter for light with a wavelength of λ is complementary, specifically including: for light with a wavelength of λ, the standard deviation of the transmittance of the notch filter and the bandpass filter is less than or equal to a set standard deviation threshold.

[0011] In combination with the first aspect, in a possible implementation manner, for light with a wavelength of λ, a standard deviation s of the summed transmittance of the notch filter and the bandpass filter is:

[0012] ;

[0013] Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ;

[0014] T(λ)=Tn(λ)×Tb(λ), where Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter, and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter.

[0015] The second aspect of the present application provides a fluorescence imaging device based on light source filtering, characterized in that the device includes: a blue light source 1, an imaging lens group 2, a notch filter 3, a photosensitive element 4, a white light source 5, a bandpass filter 6, and an image processor 7; in the photodynamic diagnosis PDD mode, the blue light source 1 is used to emit photosensitizer excitation to irradiate the observed tissue, the imaging lens group 2 is used to collect the spectrum of the PDD fluorescence excited in the observed tissue and the green fluorescence band generated by riboflavin, the notch filter 3 is used to filter the spectrum of the green fluorescence band generated by riboflavin, the PDD fluorescence passes through the notch filter 3 to reach the photosensitive element 4, and the photosensitive element 4 is used to convert the optical signal into an electrical signal and transmit it to the image processor 7 for processing, thereby outputting a PDD fluorescence image ; In the photodynamic white light imaging mode, the white light source 5 is used to emit white light, and the bandpass filter 6 is used to increase the ratio of the spectrum of the green fluorescence band in the white light to the spectrum of the red and blue light bands. After passing through the bandpass filter 6, the white light is irradiated to the observed tissue and reflected by the observed tissue to the imaging lens group 2. The imaging lens group 2 is used to collect the white light reflected by the observed tissue and transmit it to the notch filter 3. The notch filter 3 is used to filter the spectrum of the green fluorescence band and transmit it to the photosensitive element 4. The photosensitive element 4 is used to convert the optical signal into an electrical signal and transmit it to the image processor 7 for processing to output a white light image, wherein the difference between the notch filter 3 reducing the ratio of the spectrum of the green fluorescence band and the bandpass filter 6 increasing the ratio of the spectrum of the green fluorescence band is within a preset range.

[0016] In combination with the second aspect, in a possible implementation, the transmittance of the notch filter 3 for the blue band spectrum and the red band spectrum exceeds a first preset transmittance threshold, while the transmittance for the green band spectrum is lower than a second preset transmittance threshold.

[0017] In combination with the second aspect, in a possible implementation, the transmittance of the bandpass filter 6 for a green band spectrum exceeds a third preset transmittance threshold, and filters a blue band spectrum and a red band spectrum below a fourth transmittance threshold.

[0018] In combination with the second aspect, in a possible implementation manner, the transmittance of the notch filter 3 and the transmittance of the bandpass filter 6 for light with a wavelength of λ are complementary.

[0019] In combination with the second aspect, in a possible implementation, for light with a wavelength of λ, a standard deviation of the transmittance of the notch filter 3 and the bandpass filter 6 is less than or equal to a set standard deviation threshold.

[0020] In combination with the second aspect, in a possible implementation, for light with a wavelength of λ, a standard deviation s of the summed transmittance of the notch filter and the bandpass filter is:

[0021] ;

[0022] Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ;

[0023] T(λ)=Tn(λ)×Tb(λ), where Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter, and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter.

[0024] The third aspect of the present application provides a fluorescence imaging device based on light source filtering, which includes: a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, the fluorescence imaging method based on light source filtering as described in any one of the implementation methods in the first aspect to the first aspect of the present application is implemented.

[0025] The fourth aspect of the present application provides a computer-readable storage medium, which stores a computer program. When the computer program is executed by a processor, it implements the fluorescence imaging method based on light source filtering as described in any one of the implementation methods in the first aspect to the first aspect of the present application.

[0026] The present application provides a fluorescence imaging method, device and storage medium based on light source filtering, the method comprising: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue, and then the spectrum of the green fluorescence band generated by the excitation of riboflavin is filtered through a notch filter to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through a bandpass filter to increase the ratio of the green fluorescence band spectrum to the red and blue fluorescence band spectrum, and then passes through a notch filter to filter the green fluorescence band spectrum, the notch filter reduces the ratio of the green fluorescence band spectrum, and the difference between the ratio of the green fluorescence band spectrum increased by the bandpass filter is within a preset range. In this way, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging can be guaranteed. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A schematic diagram of a flow chart of a fluorescence imaging method based on light source filtering provided in an embodiment of the present application;

[0028] Figure 2 A schematic diagram of the structure of a fluorescence imaging device based on light source filtering provided in an embodiment of the present application;

[0029] Figure 3 A schematic diagram of the structure of a fluorescence imaging device based on light source filtering provided in an embodiment of the present application. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of this application.

[0031] The term "and / or" in this application can be a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this application generally indicates that the associated objects before and after are in an "or" relationship.

[0032] The terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments described herein can be implemented in an order other than that illustrated or described herein. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or modules is not necessarily limited to those steps or modules that are clearly listed, but may include other steps or modules that are not clearly listed or inherent to these processes, methods, products or devices.

[0033] During bladder PDD imaging, the metabolite riboflavin in urine will be excited by the excitation light and emit green fluorescence, thereby interfering with the contrast of PDD fluorescence. The existing method is to reduce the excitation efficiency of riboflavin by changing the excitation light band, such as using 500nm as the photosensitizer excitation wavelength. However, changing the excitation wavelength will also cause the fluorescence excitation efficiency of the photosensitizer to decrease, and the fluorescence brightness of PDD imaging will decrease. At the same time, it will cause more loss of visible light imaging band, which is not conducive to traditional white light imaging.

[0034] Therefore, the present application provides a fluorescence imaging method, device and storage medium based on light source filtering. By combining a filtering module and a light compensation module, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, while the color restoration of traditional white light imaging can be ensured.

[0035] See also Figure 1 , the present application provides a fluorescence imaging method based on light source filtering, the method comprising:

[0036] 101. Determine the working mode.

[0037] The fluorescence imaging method based on light source filtering provided in the present application is applied to a fluorescence imaging device based on light source filtering, in which a notch filter and a bandpass filter are provided.

[0038] For details, see Figure 2 The present application also provides a fluorescence imaging device based on light source filtering, which includes: a blue light source 1, an imaging lens group 2, a notch filter 3, a photosensitive element 4, a white light source 5, a bandpass filter 6, and an image processor 7.

[0039] The device has two modes: PDD fluorescence mode and photodynamic white light imaging mode. The device may also add other modes in the future, which are not limited here.

[0040] 102. In the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue and then filtered through the notch filter to filter the spectrum of the green fluorescence band produced by the excitation of riboflavin, so as to reduce the interference of green fluorescence on the PDD fluorescence contrast.

[0041] When the working mode is determined to be the PDD fluorescence mode, the blue light source 1 is turned on, and the blue light is reflected by the observed tissue to excite the fluorescence of riboflavin in the bladder. The PDD fluorescence and the spectrum of the green fluorescence band generated by the excited riboflavin are collected by the imaging lens group 2.

[0042] The notch filter 3 can be used to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin to reduce the interference of green fluorescence on the PDD fluorescence contrast. The PDD fluorescence passing through the notch filter 3 reaches the photosensitive element 4. The photosensitive element 4 then converts the optical signal into an electrical signal and transmits it to the image processor 7 for processing, thereby outputting a PDD fluorescence image.

[0043] 103. In the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through a bandpass filter to increase the ratio of the spectrum of the green fluorescence band to the spectrum of the red and blue fluorescence bands, and then passes through a notch filter to filter the spectrum of the green fluorescence band, so that the light after passing through the notch filter is still white light, wherein the difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter is within a preset range.

[0044] In the photodynamic white light imaging mode, the white light source 5 emits white light, and after the bandpass filter 6 increases the ratio of the spectrum of the green fluorescence band in the white light to the spectrum of the red and blue fluorescence bands, the white light passes through the bandpass filter 6 and is irradiated to the observed tissue, and is reflected by the observed tissue to the imaging lens group 2, which is used to collect the white light reflected by the observed tissue and transmit it to the notch filter 3, which filters the spectrum of the green fluorescence band and transmits it to the photosensitive element 4, which then converts the optical signal into an electrical signal and transmits it to the image processor 7 for processing, thereby outputting a white light image.

[0045] The difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter 3 and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter 6 is within a preset range. Exemplarily, the difference between the absorption amount of the spectrum of the green fluorescence band by the notch filter 3 and the increase amount of the spectrum of the green fluorescence band by the bandpass filter 6 can be within 10%, which is only an example and is not limited here.

[0046] It should be noted that the ratio of the green fluorescence band spectrum reduced by the notch filter 3 is specifically the ratio of the green fluorescence band spectrum reduced by the notch filter relative to the red and blue light band spectrum. The ratio of the green fluorescence band spectrum increased by the bandpass filter 6 is specifically the ratio of the green fluorescence band spectrum increased by the bandpass filter 6 relative to the red and blue light band spectrum.

[0047] It should be noted that the notch filter 3 is highly transparent to the blue band spectrum and the red band spectrum, and partially filters the green band spectrum. Specifically, the transmittance of the notch filter 3 for the blue band spectrum and the red band spectrum exceeds the first preset transmittance threshold. And the transmittance for the green band spectrum is lower than the second preset transmittance threshold. Exemplarily, the first preset transmittance threshold and the second preset transmittance threshold can be set to 90% and 10%, respectively. It can also be set to 50%, 60%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the notch filter. The green band spectrum filtered by the notch filter is between 500 nanometers and 575 nanometers.

[0048] The bandpass filter 6 is highly transparent to the green band spectrum, while partially filtering the blue band spectrum and the red band spectrum. Specifically, the transmittance of the bandpass filter 6 for the green band spectrum exceeds the third preset transmittance threshold, while filtering the blue band spectrum and the red band spectrum is lower than the fourth transmittance threshold. Exemplarily, the third preset transmittance threshold and the fourth preset transmittance threshold can be set to 90% and 10%, respectively. It can also be set to 50%, 60%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the bandpass filter.

[0049] It should be noted that the transmittance of the notch filter 3 and the bandpass filter 6 for light with a wavelength of λ is complementary. Specifically, for light with a wavelength of λ, the standard deviation of the transmittance of the notch filter 3 and the bandpass filter 6 is less than or equal to a set standard deviation threshold. Exemplarily, the standard deviation threshold can be set to 10%, but is not limited thereto.

[0050] For light with a wavelength of λ, the standard deviation s of the transmittance of the notch filter 3 and the bandpass filter 6 is:

[0051] ;

[0052] Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ;

[0053] T(λ)=Tn(λ)×Tb(λ), wherein Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter 3 , and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter 6 .

[0054] This application also provides a fluorescence imaging device based on light source filtering, see Figure 2 The device includes: a blue light source 1, an imaging lens group 2, a notch filter 3, a photosensitive element 4, a white light source 5, a bandpass filter 6, and an image processor 7.

[0055] In the photodynamic diagnosis PDD mode, the blue light source 1 is used to emit photosensitizer excitation to the observed tissue, the imaging lens group 2 is used to collect the spectrum of the PDD fluorescence excited in the observed tissue and the green fluorescence band produced by riboflavin excitation, the notch filter 3 is used to filter the spectrum of the green fluorescence band produced by riboflavin excitation, the PDD fluorescence passes through the notch filter 3 to reach the photosensitive element 4, the photosensitive element 4 is used to convert the optical signal into an electrical signal and transmit it to the image processor 7 for processing, thereby outputting a PDD fluorescence image.

[0056] In the photodynamic white light imaging mode, the white light source 5 is used to emit white light, and the bandpass filter 6 is used to increase the ratio of the spectrum of the green fluorescence band in the white light to the spectrum of the red and blue light bands. After passing through the bandpass filter 6, the white light is irradiated to the observed tissue and reflected by the observed tissue to the imaging lens group 2. The imaging lens group 2 is used to collect the white light reflected by the observed tissue and transmit it to the notch filter 3. The notch filter 3 is used to filter the spectrum of the green fluorescence band and transmit it to the photosensitive element 4. The photosensitive element 4 is used to convert the optical signal into an electrical signal and transmit it to the image processor 7 for processing to output a white light image, wherein the difference between the notch filter 3 reducing the ratio of the spectrum of the green fluorescence band and the bandpass filter 6 increasing the ratio of the spectrum of the green fluorescence band is within a preset range.

[0057] It should be noted that the ratio of the green fluorescence band spectrum reduced by the notch filter 3 is specifically the ratio of the green fluorescence band spectrum reduced by the notch filter relative to the red and blue light band spectrum. The ratio of the green fluorescence band spectrum increased by the bandpass filter 6 is specifically the ratio of the green fluorescence band spectrum increased by the bandpass filter 6 relative to the red and blue light band spectrum. It should be noted that the notch filter 3 is highly transparent to the blue band spectrum and the red band spectrum, and partially filters the green band spectrum. Specifically, the transmittance of the notch filter 3 for the blue band spectrum and the red band spectrum exceeds the first preset transmittance threshold. And the transmittance for the green band spectrum is lower than the second preset transmittance threshold. Exemplarily, the first preset transmittance threshold and the second preset transmittance threshold can be set to 90% and 10% respectively. It can also be set to 50%, 60%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the notch filter. The green band spectrum filtered by this notch filter is between 500 nanometers and 575 nanometers.

[0058] The bandpass filter 6 is highly transparent to the green band spectrum, while partially filtering the blue band spectrum and the red band spectrum. Specifically, the transmittance of the bandpass filter 6 for the green band spectrum exceeds the third preset transmittance threshold, while filtering the blue band spectrum and the red band spectrum is lower than the fourth transmittance threshold. Exemplarily, the third preset transmittance threshold and the fourth preset transmittance threshold can be set to 90% and 10%, respectively. It can also be set to 50%, 60%, etc., which can be set according to actual business needs and is not limited here. The setting of the transmittance can be achieved by coating the surface of the bandpass filter.

[0059] It should be noted that the transmittance of the notch filter 3 and the bandpass filter 6 for light with a wavelength of λ is complementary. Specifically, for light with a wavelength of λ, the standard deviation of the transmittance of the notch filter 3 and the bandpass filter 6 is less than or equal to a set standard deviation threshold. Exemplarily, the standard deviation threshold can be set to 10%, but is not limited thereto.

[0060] For light with a wavelength of λ, the standard deviation s of the transmittance of the notch filter 3 and the bandpass filter 6 is:

[0061] ;

[0062] Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ;

[0063] T(λ)=Tn(λ)×Tb(λ), wherein Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter 3 , and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter 6 .

[0064] The present application provides a fluorescence imaging method, device and storage medium based on light source filtering, the method comprising: in the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue, and then the spectrum of the green fluorescence band generated by the excitation of riboflavin is filtered through the notch filter 3 to reduce the interference of green fluorescence on the PDD fluorescence contrast; in the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through the bandpass filter 6 to enhance the spectrum of the green fluorescence band, and then passes through the notch filter 3 to filter the spectrum of the green fluorescence band, so that the light after passing through the notch filter 3 is still white light, wherein the notch filter 3 reduces the proportion of the spectrum of the green fluorescence band, and the difference between the proportion of the spectrum of the green fluorescence band increased by the bandpass filter 6 is within a preset range. In this way, the problem of the green fluorescence generated by the excitation of riboflavin affecting the contrast of PDD fluorescence imaging during PDD fluorescence imaging is solved, and the color restoration of traditional white light imaging can be guaranteed at the same time.

[0065] Figure 3 Schematic diagram of the structure of the fluorescence imaging device 20 based on light source filtering provided in the embodiment of the present application. Figure 3 As shown, the fluorescence imaging device 20 based on light source filtering of this embodiment includes: at least one processor 201 ( Figure 3Only one processor is shown in the figure), a memory 202 and a computer program 203 stored in the memory 202 and executable on the at least one processor 201, wherein the processor 201 implements the steps in any of the above-mentioned embodiments of the fluorescence imaging method based on light source filtering when executing the computer program 203.

[0066] The fluorescence imaging device 20 based on light source filtering may include, but is not limited to, a processor 201 and a memory 202. Those skilled in the art will appreciate that Figure 3 It is only an example of the fluorescence imaging device 20 based on light source filtering and does not constitute a limitation of the fluorescence imaging device 20 based on light source filtering. It may include more or fewer components than shown in the figure, or a combination of certain components, or different components, for example, it may also include input and output devices, network access devices, etc.

[0067] The processor 201 may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or any conventional processor, etc.

[0068] In some embodiments, the memory 202 may be an internal storage unit of the fluorescence imaging device 20 based on light source filtering, such as a hard disk or memory of the fluorescence imaging device 20 based on light source filtering. In other embodiments, the fluorescence imaging device 20 based on light source filtering may also be an external storage device of the fluorescence imaging device 20 based on light source filtering, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (Secure Digital, SD) card, a flash card (Flash Card), etc. equipped on the fluorescence imaging device 20 based on light source filtering. Further, the memory 202 may also include both the internal storage unit of the fluorescence imaging device 20 based on light source filtering and an external storage device. The memory 202 is used to store operating devices, application programs, boot loaders (BootLoader), data and other programs, such as program codes of the computer program, etc. The memory 202 may also be used to temporarily store data that has been output or is to be output.

[0069] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0070] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application is essentially or the part that contributes to the prior art or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a storage medium, including several instructions to enable a computer device (which can be a personal computer, server, or network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM), random access memory (RAM), disk or optical disk, etc., various media that can store program codes.

[0071] In the embodiments provided in this application, it should be understood that the disclosed methods can be implemented in other ways without exceeding the spirit and scope of this application. The current embodiment is only an illustrative example and should not be used as a limitation. The specific content given should not limit the purpose of this application. For example, some features can be ignored or not executed.

[0072] The technical means disclosed in the present application are not limited to the technical means disclosed in the above-mentioned implementation methods, but also include technical solutions composed of any combination of the above technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of this application, and these improvements and modifications are also regarded as the protection scope of this application.

[0073] The above is a detailed introduction to a fluorescence imaging method, device and storage medium based on light source filtering provided in the embodiments of the present application. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea; at the same time, for those of ordinary skill in the art, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application. Although the present application has been described in detail with reference to the aforementioned embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein; and these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A fluorescence imaging method based on light source filtering, characterized in that: The method is applied to a fluorescence imaging device based on light source filtering, wherein a notch filter and a bandpass filter are provided in the device, and the device has two modes: a photodynamic diagnosis PDD fluorescence mode and a photodynamic white light imaging mode. The method comprises: In the PDD fluorescence mode, when the blue light source is turned on, the blue light is reflected by the observed tissue and then filtered through the notch filter to filter the spectrum of the green fluorescence band generated by the excitation of riboflavin, so as to reduce the interference of green fluorescence on the PDD fluorescence contrast; In the photodynamic white light imaging mode, when the white light source is turned on, the white light first passes through a bandpass filter to increase the ratio of the spectrum of the green fluorescence band to the spectrum of the red and blue fluorescence bands, and then passes through a notch filter to filter the spectrum of the green fluorescence band after being reflected by the observed tissue, so that the light after passing through the notch filter is still white light, wherein the difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter is within a preset range; The transmittance of the notch filter for the blue band spectrum and the red band spectrum exceeds a first preset transmittance threshold, while the transmittance for the green band spectrum is lower than a second preset transmittance threshold; The transmittance of the bandpass filter for the green band spectrum exceeds the third preset transmittance threshold, while the transmittance of the blue band spectrum and the red band spectrum is lower than the fourth transmittance threshold; The difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter is within a preset range and specifically includes: The transmittance of the notch filter and the bandpass filter for light with a wavelength of λ are complementary; The transmittance of the notch filter and the bandpass filter for light with a wavelength of λ is complementary, specifically including: The standard deviation s of the transmittance of the notch filter and the bandpass filter is less than or equal to a set standard deviation threshold; ； Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ； T(λ)=Tn(λ)×Tb(λ), where Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter, and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter.

2. A fluorescence imaging device based on light source filtering, characterized in that: The device comprises: a blue light source (1), an imaging lens group (2), a notch filter (3), a photosensitive element (4), a white light source (5), a bandpass filter (6), and an image processor (7); In a photodynamic diagnosis PDD mode, the blue light source (1) is used to emit photosensitizer excitation to irradiate the observed tissue, the imaging lens group (2) is used to collect the spectrum of the PDD fluorescence generated by the observed tissue and the green fluorescence band generated by the excited riboflavin, the notch filter (3) is used to filter the spectrum of the green fluorescence band generated by the excited riboflavin, the PDD fluorescence passes through the notch filter (3) to reach the photosensitive element (4), and the photosensitive element (4) is used to convert the optical signal into an electrical signal and transmit it to the image processor (7) for processing, thereby outputting a PDD fluorescence image; In the photodynamic white light imaging mode, the white light source (5) is used to emit white light, the bandpass filter (6) is used to increase the ratio of the spectrum of the green fluorescence band in the white light to the spectrum of the red and blue light bands, the white light passes through the bandpass filter (6) and then irradiates the observed tissue, and is reflected by the observed tissue to the imaging lens group (2), the imaging lens group (2) is used to collect the white light reflected by the observed tissue and transmit it to the notch filter (3), the notch filter (3) is used to filter the spectrum of the green fluorescence band and transmit it to the photosensitive element (4), the photosensitive element (4) is used to convert the optical signal into an electrical signal and transmit it to the image processor (7) for processing to output a white light image, wherein the difference between the ratio of the spectrum of the green fluorescence band reduced by the notch filter (3) and the ratio of the spectrum of the green fluorescence band increased by the bandpass filter (6) is within a preset range; The transmittance of the notch filter (3) for the blue band spectrum and the red band spectrum exceeds a first preset transmittance threshold, while the transmittance for the green band spectrum is lower than a second preset transmittance threshold; The bandpass filter (6) has a transmittance greater than a third preset transmittance threshold for a green wavelength band spectrum, and filters a blue wavelength band spectrum and a red wavelength band spectrum below a fourth transmittance threshold; The transmittance of the notch filter (3) and the bandpass filter (6) for light with a wavelength of λ are complementary; The standard deviation s of the transmittance of the notch filter (3) and the bandpass filter (6) is less than or equal to a set standard deviation threshold; ； Where T(λ) is the transmittance of light with a wavelength of λ. is the mean value of the same and light transmittance; ； T(λ)=Tn(λ)×Tb(λ), wherein Tn(λ) is the transmittance of light with a wavelength of λ entering the notch filter (3), and Tb(λ) is the transmittance of light with a wavelength of λ entering the bandpass filter (6).

3. A fluorescence imaging device based on light source filtering, characterized in that: The device comprises: a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, the fluorescence imaging method based on light source filtering as claimed in claim 1 is implemented.

4. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the fluorescence imaging method based on light source filtering as claimed in claim 1 is implemented.

Images