B-ultrasonic degradable coupling patch capable of avoiding cross infection and preparation method of B-ultrasonic degradable coupling patch

Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to a B-ultrasonic degradable coupling patch capable of avoiding cross infection and a preparation method of the B-ultrasonic degradable coupling patch. The B-ultrasonic degradable coupling patch capable of avoiding cross infection has acoustic performance and antibacterial performance and can perfectly replace a traditional liquid coupling agent, in addition, the modified guar gum, the coral mucus and the oxidized glucose are all natural degradable materials subjected to certain treatment, and compared with an existing solid coupling patch, the coupling patch has the advantages that the cost is low, and the application range is wide. Acoustic performance and antibacterial performance are both considered, and the fabric is more environmentally friendly and safer.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and specifically relates to a degradable coupling patch for B-ultrasound to avoid cross infection and a preparation method thereof. Background Art

[0002] B-ultrasound, also known as B-type ultrasound, is a two-dimensional grayscale ultrasound, which is a type of ultrasound examination technology. The color ultrasound instrument currently widely used in China includes B-type ultrasound and color Doppler ultrasound functions. Equipment that uses B-ultrasound examination alone has been basically eliminated. B-ultrasound examination is a non-surgical diagnostic examination. Ultrasound has multiple physical properties such as reflection, refraction, scattering, attenuation, and absorption. When it passes through different tissues and organs of the human body, it produces echo signals of different intensities due to different acoustic impedances. After these echo signals are received by the ultrasound probe, they are processed by the computer and presented on the display in various sound images. Doctors make a diagnosis of the disease by analyzing the sound images. Therefore, in the operation of ultrasound diagnosis and treatment, it is necessary to fill the medical ultrasound coupling agent between the radiation surface of the ultrasound probe and the skin. Medical ultrasound coupling agent is a necessary auxiliary preparation for the probe of ultrasound diagnostic instruments. It is used to fill the intermediate medium for transmitting sound waves between the radiation surface of the ultrasound probe and the skin. It is the propagation channel of ultrasound. The quality of the coupling agent directly affects the image clarity and resolution.

[0003] YY0299-2008 "Medical Ultrasonic Coupling Agent" points out that medical ultrasonic coupling agent products are water-based polymer gel-type preparations. During ultrasonic diagnosis and treatment operations, the gel-like medical ultrasonic coupling agent needs to be applied to the ultrasonic probe or the skin of the detection site. After the ultrasonic detection is completed, it needs to be washed with water or paper to remove the residual coupling agent. The operation is neither convenient nor hygienic. In addition, the gel-like medical ultrasonic coupling agent is not effective when used on curved and tortuous parts of the body such as wrists and ankles. At the same time, it is not suitable for contact with damaged skin.

[0004] In addition, during ultrasound diagnosis and treatment operations, the ultrasound probe directly contacts the patient's skin and needs to be disinfected promptly and regularly after use. Clinically, unsterilized probes are found to contain common pathogens, which poses a potential risk of medical cross-infection. According to relevant specifications, all diagnostic and treatment instruments that contact the skin must be effectively disinfected, including ultrasound probes. However, due to the particularity of ultrasound probe materials and structures, an ideal ultrasound probe disinfection method has not yet been found. Wiping with peracetic acid, 75% ethanol, or chlorine-containing disinfectants often deforms and cracks the probe, shortening its service life; iodine tincture is easy to color and can affect sound transmission performance; ultraviolet irradiation disinfection can interfere with ultrasound and affect probe performance; liquid spraying, gas fumigation, and ultra-thin plastic paper wrapping are inconvenient for daily operations and are not easy to promote and implement for a long time.

[0005] Solid ultrasonic coupling agents that have emerged in recent years can overcome the above-mentioned defects of liquid ultrasonic coupling agents and can be used on any part of the body surface. In the prior art, solid ultrasonic coupling agents are mostly made of water-based polymer materials as the main component, with certain moisturizers, preservatives, colorants and other substances added, and then solidified. Currently, silver nanoparticles, photoresponsive materials, antimicrobial peptides, cationic polymers, etc. are mainly used as antimicrobial agents. However, these antibacterial hydrogels also have some shortcomings, such as silver nanoparticles may cause cytotoxicity and environmental pollution; photoresponsive materials require external light sources to activate; antimicrobial peptides may be degraded by proteases or bind to serum proteins; cationic polymers may cause hemolysis or inflammatory reactions. Therefore, it is an urgent need in the current field of ultrasonic medicine to develop an antibacterial molecule that is efficient, safe, stable and environmentally friendly, and use it to prepare an ultrasonic coupling agent with antibacterial activity. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a degradable coupling patch for B-ultrasound that can avoid cross infection and a preparation method thereof.

[0007] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials: glycerin, modified guar gum, coral mucus, oxidized glucose, konjac flour, carrageenan and water.

[0008] Preferably, the degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 2-5% glycerol, 2-4% modified guar gum, 5-10% coral mucus, 2-4% oxidized glucose, 0.1-0.5% konjac flour, 0.1-0.5% carrageenan, and the balance is water.

[0009] Further preferably, the degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials, by mass percentage: 2-3% glycerol, 2.6-3.2% modified guar gum, 6-8% coral mucus, 2.6-3.2% oxidized glucose, 0.2-0.4% konjac flour, 0.1-0.3% carrageenan, and the balance is water.

[0010] The preparation method of the modified guar gum comprises the following steps:

[0011] 1-2 parts of guar gum are added to 20-40 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 80-120 r / min for 10-20 minutes. Then, 0.9-1 parts of N-hydroxysuccinimide and 0.5-0.6 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, and stirring is continued for 10-30 minutes. Subsequently, 1-1.4 parts of 3,4-dihydroxyphenylacetic acid are added, and stirring is continued for 6-8 hours under a nitrogen atmosphere. The mixture is centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0012] The freeze drying temperature is -20°C to -10°C, and the vacuum degree is 10 -2 Up to 10 -3 mbar.

[0013] The method for extracting coral mucus comprises the following steps:

[0014] Take a piece of 200-500cm 2 The living coral is stimulated by letting a weight of 0.5-1.5 kg fall freely from 20-30 cm above the living coral for 1-3 times, and then the stimulated living coral is immersed in seawater for 50-100 minutes, and finally the living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1: (0.01-0.1), stirred at a speed of 80-200 r / min for 5-10 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0015] The method for preparing oxidized glucose comprises the following steps:

[0016] By mass, 1-2 parts of dextran are added to 90-110 parts of water, stirred at 80-120 r / min for 5-10 min, then 1-1.5 parts of sodium periodate are added, stirring is continued in a dark environment for 6-8 h, then 1-2 parts of ethylene glycol are added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0017] The freeze drying temperature is -40°C to -20°C, and the vacuum degree is 10 -2 Up to 10 -3 mbar.

[0018] The present invention also provides a method for preparing a degradable coupling patch for B-ultrasound that avoids cross infection, comprising the following steps:

[0019] (1) mixing modified guar gum, coral mucus, konjac flour, carrageenan, and water according to mass percentage, and stirring at a speed of 80-120 r / min for 10-20 min to obtain solution A;

[0020] (2) Adding glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heating to 60-80° C. and continuing stirring for 25-30 min, finally vacuuming and defoaming for 6-10 min, casting, naturally curing for 8-12 h, and then microwave drying at 40-60° C. for 5-10 min, rolling and cutting to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5-2 mm.

[0021] The power of the microwave drying is 12-48KW.

[0022] The present invention uses modified guar gum, coral mucus, and oxidized glucose as main raw materials, and grafts guar gum with 3,4-dihydroxyphenylacetic acid, which can effectively reduce its viscosity. Compared with the traditional hydrogel synthesized with carrageenan, xanthan gum, and guar gum as raw materials, it has more excellent acoustic performance. In addition, the guar gum grafted with 3,4-dihydroxyphenylacetic acid can react with oxidized glucose to form a hydrogel with a relatively excellent chemical structure, which can well lock the coral mucus therein.

[0023] In addition, the mucus layer on the surface of corals provides an important interface between the coral epithelium and the seawater environment. The mucus plays a defensive role in resisting various environmental pressures, ciliary mucus feeding and sediment cleaning. On the one hand, suspended particles flowing through the reef are bound in sticky clumps and quickly settle and are decomposed by benthic organisms. Provide nutrients for the growth of corals; on the other hand, the mucus on the surface of corals can play a cementing role, bonding coral fragments together. Compared with carrageenan, xanthan gum, and guar gum, its lower viscosity can further reduce the density of the formed hydrogel and promote the formation of the gel structure, which is also conducive to improving the acoustic performance of the obtained degradable coupling patch for B-ultrasound to avoid cross infection.

[0024] Furthermore, coral mucus contains natural antibacterial ingredients that can effectively inhibit bacterial growth. 3,4-dihydroxyphenylacetic acid grafted guar gum also has good antibacterial properties. The two work together to effectively inhibit bacterial growth in the coupling patch and avoid cross infection.

[0025] Beneficial effects of the present invention:

[0026] The degradable coupling patch for B-ultrasound that avoids cross infection provided by the present invention takes into account both acoustic performance and antibacterial performance, and can perfectly replace traditional liquid coupling agents. In addition, modified guar gum, coral mucus, and oxidized glucose are all natural degradable materials that have been processed to a certain extent. Compared with the existing solid coupling patch, it takes into account both acoustic performance and antibacterial performance, and is more environmentally friendly and safe. DETAILED DESCRIPTION

[0027] The present invention will be further described below with reference to the embodiments and comparative examples.

[0028] The coral used in the embodiment is a giant symphylla coral from the sea area of ​​Sanya, Hainan.

[0029] Guar gum, product number: 2202, Shandong Dongrun Biotechnology Co., Ltd.

[0030] The power of the microwave drying in the embodiments and comparative examples is 12-48KW.

[0031] Example 1

[0032] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 8% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0033] The preparation method of the modified guar gum comprises the following steps:

[0034] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0035] The method for extracting coral mucus comprises the following steps:

[0036] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0037] The method for preparing oxidized glucose comprises the following steps:

[0038] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0039] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection, comprising the following steps:

[0040] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0041] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0042] Example 2

[0043] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 6% modified guar gum, 8% coral mucus, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0044] The preparation method of the modified guar gum comprises the following steps:

[0045] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0046] The method for extracting coral mucus comprises the following steps:

[0047] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0048] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection comprises the following steps:

[0049] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0050] (2) adding glycerol to the solution A obtained in step (1) according to the mass percentage, heating to 80° C. and continuing stirring for 30 min, finally vacuuming and defoaming for 8 min, casting, naturally curing for 10 h, and then microwave drying at 50° C. for 8 min, and rolling and cutting to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0051] Example 3

[0052] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0053] The preparation method of the modified guar gum comprises the following steps:

[0054] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0055] The method for preparing oxidized glucose comprises the following steps:

[0056] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0057] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection comprises the following steps:

[0058] (1) Mixing modified guar gum, konjac flour, carrageenan, and water according to mass percentage, and stirring at a speed of 100 r / min for 15 min to obtain solution A;

[0059] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0060] Example 4

[0061] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 12% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0062] The preparation method of the modified guar gum comprises the following steps:

[0063] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0064] The method for extracting coral mucus comprises the following steps:

[0065] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0066] The method for preparing oxidized glucose comprises the following steps:

[0067] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0068] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection comprises the following steps:

[0069] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0070] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0071] Example 5

[0072] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 4% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0073] The preparation method of the modified guar gum comprises the following steps:

[0074] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0075] The method for extracting coral mucus comprises the following steps:

[0076] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0077] The method for preparing oxidized glucose comprises the following steps:

[0078] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0079] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection comprises the following steps:

[0080] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0081] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0082] Comparative Example 1

[0083] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% guar gum, 8% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0084] The method for extracting coral mucus comprises the following steps:

[0085] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0086] The method for preparing oxidized glucose comprises the following steps:

[0087] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0088] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection comprises the following steps:

[0089] (1) guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0090] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0091] Comparative Example 2

[0092] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 8% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0093] The preparation method of the modified guar gum comprises the following steps:

[0094] By mass, 1 part of guar gum was added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes. Then, 1.46 parts of N-hydroxysuccinimide were added, and stirring was continued for 20 minutes. Subsequently, 1.2 parts of 3,4-dihydroxyphenylacetic acid were added, and stirring was continued for 7 hours under a nitrogen atmosphere. The mixture was centrifuged and filtered, and the supernatant was freeze-dried to obtain the modified guar gum.

[0095] The method for extracting coral mucus comprises the following steps:

[0096] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0097] The method for preparing oxidized glucose comprises the following steps:

[0098] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0099] A method for preparing a degradable coupling patch for B-ultrasound that avoids cross infection comprises the following steps:

[0100] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0101] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0102] Comparative Example 3

[0103] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 8% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0104] The preparation method of the modified guar gum comprises the following steps:

[0105] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, then 1.46 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, and stirring is continued for 20 minutes, followed by adding 1.2 parts of 3,4-dihydroxyphenylacetic acid, and stirring is continued for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0106] The method for extracting coral mucus comprises the following steps:

[0107] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0108] The method for preparing oxidized glucose comprises the following steps:

[0109] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0110] A method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection, comprising the following steps:

[0111] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0112] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0113] Comparative Example 4

[0114] A degradable coupling patch for B-ultrasound to avoid cross infection is composed of the following raw materials by mass percentage: 3% glycerol, 3% modified guar gum, 8% coral mucus, 3% oxidized glucose, 0.3% konjac flour, 0.2% carrageenan, and the balance is water.

[0115] The preparation method of the modified guar gum comprises the following steps:

[0116] By mass, 1 part of guar gum is added to 20 parts of acetic acid aqueous solution with a volume fraction of 2%, and stirred at a speed of 100 r / min for 15 minutes, followed by adding 0.92 parts of N-hydroxysuccinimide and 0.54 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and stirring is continued for 20 minutes. Subsequently, 1.2 parts of 2,5-dihydroxyphenylacetic acid are added, and the reaction is continued to be stirred for 7 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

[0117] The method for extracting coral mucus comprises the following steps:

[0118] Take a 300cm 2 A living coral is stimulated by letting a 1 kg weight fall freely from 25 cm above the living coral twice, and then the stimulated living coral is immersed in seawater for 80 minutes. Finally, the stimulated living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1:0.05, stirred at a speed of 120 r / min for 8 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

[0119] The method for preparing oxidized glucose comprises the following steps:

[0120] By mass, 1 part of dextran was added to 100 parts of water, stirred at 100 r / min for 8 min, then 1.2 parts of sodium periodate was added, stirring was continued for 6 h in a dark environment, then 1 part of ethylene glycol was added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

[0121] A method for preparing a degradable coupling patch for B-ultrasound that avoids cross infection comprises the following steps:

[0122] (1) Modified guar gum, coral mucus, konjac flour, carrageenan, and water were mixed according to mass percentage, and stirred at a speed of 100 r / min for 15 min to obtain solution A;

[0123] (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 80° C. and continue stirring for 30 min, finally evacuate and defoam for 8 min, cast, naturally cure for 10 h, and then microwave dry at 50° C. for 8 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5 mm.

[0124] Test Example 1:

[0125] The main performance indicators of the degradable coupling patch for B-ultrasound to avoid cross infection prepared in Examples 1-5 and Comparative Example 1 were tested with reference to the testing method of YY / T0299-2022, and the results are shown in Table 1.

[0126] Table 1: Acoustic performance test results

[0127]

[0128]

[0129] Test Example 2

[0130] The test was conducted in accordance with the requirements of 2.1.1 Disinfectant Microbial Killing Test in the 2002 edition of the Technical Specifications for Disinfection. Staphylococcus aureus ATCC6538 was selected as the test bacteria, and the results are shown in Table 2.

[0131] Table 2: Results of bactericidal test on Staphylococcus aureus

[0132] Killing logarithm value at 3.0min Example 1 ≥5.0 Example 2 4.9 Example 3 3.0 Example 4 ≥5.0 Example 5 4.3 Comparative Example 1 4.6 Comparative Example 2 4.8 Comparative Example 3 4.8 Comparative Example 4 4.7

[0133] As can be seen from Table 1, the sound velocity of the degradable coupling patch for B-ultrasound to avoid cross infection synthesized by glycerol, modified guar gum, coral mucus, oxidized glucose and water in Examples 1-5 of the present invention is between 1573-1586 m / s, and the acoustic characteristic impedance is between 1.57×10 6 -1.60×106 Pa.s / m, and the slope of the acoustic attenuation coefficient is between 0.048-0.051dB / cm.MHz, which meets the standards of the "Pharmaceutical Industry Standard of the People's Republic of China YY0299-2008" for medical ultrasonic coupling agents (sound velocity of 1520-1620m / s, acoustic characteristic impedance of 1.5×106-1.7×106Pa.s / m, and slope of the acoustic attenuation coefficient ≤0.05dB / cm.MHz), and has excellent acoustic performance. It can be seen that modified guar gum, oxidized glucose, and coral mucus have a certain influence on the acoustic performance of the prepared degradable coupling patch for B-ultrasound to avoid cross infection. Among them, the effect of guar gum is the most obvious. By comparing the embodiment and comparative example 1, it can be seen that the modified guar gum has a significantly smaller effect on the acoustic performance. The acoustic performance is related to the chemical structure and density of the product. The present invention believes that the modification process can effectively reduce the viscosity of guar gum, thereby reducing its density, thereby obtaining a degradable coupling patch for B-ultrasound with excellent acoustic performance to avoid cross infection. In addition, the concentration of modified guar gum, oxidized glucose, and coral mucus also has a certain effect on the acoustic performance. The higher the concentration, the greater the density of the product, and the worse its acoustic performance.

[0134] As can be seen from Table 2, the antibacterial effect is mainly played by modified guar gum and coral mucus, and the two can play an excellent antibacterial effect at a suitable addition amount. In the process of modifying guar gum, the present invention finds that hydroxyphenylacetic acid modification can effectively reduce the density of guar gum, improve its acoustic properties, and give it stronger antibacterial ability. It can be seen from Example 1 and Comparative Example 4 that 3,4-dihydroxyphenylacetic acid modification has a better antibacterial effect. It can be seen from Example 1 and Comparative Example 2 and Comparative Example 3 that N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride work together, which helps 3,4-dihydroxyphenylacetic acid to modify guar gum and can further enhance its antibacterial performance. The present invention believes that this is because N-hydroxysuccinimide (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) work together to generate NHS esters, which are more easily reacted with amines than free carboxyl groups, thereby promoting the formation of amide bonds.

[0135] Therefore, the degradable coupling patch for B-ultrasound that avoids cross infection provided by the present invention takes into account both acoustic performance and antibacterial performance, and can perfectly replace traditional liquid coupling agents. In addition, modified guar gum, coral mucus, and oxidized glucose are all natural degradable materials that have undergone certain treatments. Compared with existing solid-state coupling patches, they take into account both acoustic performance and antibacterial performance, and are more environmentally friendly and safe.

Claims

1. A degradable coupling patch for B-ultrasound to avoid cross infection, characterized in that: It is composed of the following ingredients: glycerin, modified guar gum, coral mucus, oxidized glucose, konjac flour, carrageenan, and water.

2. The degradable coupling patch for B-ultrasound to avoid cross infection as claimed in claim 1, characterized in that: The invention is composed of the following raw materials by mass percentage: 2-5% glycerol, 2-4% modified guar gum, 5-10% coral mucus, 2-4% oxidized glucose, 0.1-0.5% konjac flour, 0.1-0.5% carrageenan, and the balance is water.

3. The degradable coupling patch for B-ultrasound to avoid cross infection according to any one of claims 1 to 2, characterized in that: The invention is composed of the following raw materials by mass percentage: 2-3% glycerol, 2.6-3.2% modified guar gum, 6-8% coral mucus, 2.6-3.2% oxidized glucose, 0.2-0.4% konjac flour, 0.1-0.3% carrageenan, and the balance is water.

4. The degradable coupling patch for B-ultrasound to avoid cross infection according to any one of claims 1 to 3, characterized in that: The preparation method of the modified guar gum comprises the following steps: By mass, 1-2 parts of guar gum are added to 20-40 parts of acetic acid aqueous solution, stirred at a speed of 80-120 r / min for 10-20 minutes, then 0.9-1 parts of N-hydroxysuccinimide and 0.5-0.6 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, stirring is continued for 10-30 minutes, and then 1-1.4 parts of 3,4-dihydroxyphenylacetic acid are added, stirring is continued for 6-8 hours under a nitrogen atmosphere, centrifuged, filtered, and the supernatant is freeze-dried to obtain the modified guar gum.

5. The degradable coupling patch for B-ultrasound to avoid cross infection as claimed in claim 4, characterized in that: The volume fraction of the acetic acid aqueous solution is 2%.

6. The degradable coupling patch for B-ultrasound to avoid cross infection as claimed in claim 4, characterized in that: The freeze drying temperature is -20°C to -10°C, and the vacuum degree is 10 -2 Up to 10 -3 mbar.

7. The degradable coupling patch for B-ultrasound to avoid cross infection according to any one of claims 1 to 3, characterized in that: The method for extracting coral mucus comprises the following steps: Take a piece of 200-500cm 2 The living coral is stimulated by letting a weight of 0.5-1.5 kg fall freely from 20-30 cm above the living coral for 1-3 times, and then the stimulated living coral is immersed in seawater for 50-100 minutes, and finally the living coral is taken out and inverted to collect mucus, and the obtained mucus and activated carbon particles are mixed at a mass ratio of 1: (0.01-0.1), stirred at a speed of 80-200 r / min for 5-10 minutes, and the activated carbon is separated by centrifugation to obtain the coral mucus.

8. The degradable coupling patch for B-ultrasound to avoid cross infection according to any one of claims 1 to 3, characterized in that: The method for preparing oxidized glucose comprises the following steps: By mass, 1-2 parts of dextran are added to 90-110 parts of water, stirred at 80-120 r / min for 5-10 min, then 1-1.5 parts of sodium periodate are added, stirring is continued in a dark environment for 6-8 h, then 1-2 parts of ethylene glycol are added to terminate the reaction, and freeze-dried to obtain the oxidized glucose.

9. The degradable coupling patch for B-ultrasound to avoid cross infection as claimed in claim 8, characterized in that: The freeze drying temperature is -40°C to -20°C, and the vacuum degree is 10 -2 Up to 10 -3 mbar.

10. The method for preparing a degradable coupling patch for B-ultrasound to avoid cross infection according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) mixing modified guar gum, coral mucus, konjac flour, carrageenan, and water according to mass percentage, and stirring at a speed of 80-120 r / min for 10-20 min to obtain solution A; (2) Add glycerol and oxidized glucose to the solution A obtained in step (1) according to the mass percentage, heat to 60-80° C. and continue stirring for 25-30 min, finally vacuum defoam for 6-10 min, cast into a film, naturally cure for 8-12 h, and then microwave dry at 40-60° C. for 5-10 min, roll and cut to obtain the degradable coupling patch for B-ultrasound to avoid cross infection; the thickness of the degradable coupling patch for B-ultrasound to avoid cross infection is 0.5-2 mm.

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