Pesticidal compositions comprising isoxazoline compounds

Abstract

The present invention relates to a pesticidal composition comprising component A and component B, characterized in that the component A is isoxtolfenpyrad and the component B is a component selected from the group consisting of Bacillus spp., fermented solids thereof, and insecticidal toxins thereof.

Description

[0001] The present invention relates to compositions of pesticidal active ingredients and to methods of using these compositions in agricultural applications.

[0002] WO 2011067272 discloses that certain isoxazoline compounds have insecticidal activity.

[0003] The present invention provides a pesticidal composition comprising component A and component B, wherein component A is isoxathiapiprolin, and component B is a component selected from Bacillus species, fermentation solids thereof, and insecticidal toxins thereof.

[0004] It has now surprisingly been found that the active ingredient combinations according to the invention, or in other words active ingredient mixtures, achieve a synergistic effect, especially for the treatment of pests from the orders Lepidoptera and / or Coleoptera. The active ingredient combinations achieve a high degree of pest control, especially in a very short time.

[0005] Throughout this document, the expression "composition" represents various mixtures or combinations of components (A) and (B), for example in a single "ready-to-use" form, in a combined spray mixture (which mixture consists of separate formulations of the individual active ingredient components) (such as a "tank mix"), and in combination with individual active ingredients when applied in a sequential manner (i.e., one after another within a reasonably short period of time, such as a few hours or days). For the implementation of the present invention, the order of applying components (A) and (B) is not important.

[0006] Isotrione is an insecticidal agricultural chemical with the following CAS number: 2061933-85-3 and has the following chemical formula:

[0007] Isoxafenamide may include isomer (5S, 4R), which is 4-[(5S)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4R)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide, and optionally at least one isomer selected from isomer (5S, 4S), isomer (5R, 4R), isomer (5R, 4S), and any combination thereof. In the present invention, the isomer (5S, 4S) is 4-[(5S)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4S)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide; the isomer (5R, 4R) is 4-[(5R)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)- isomer (5R,4S) is 4-[(5R)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4S)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide. When isoxathiapiprolin further comprises at least one isomer selected from isomer (5S, 4S), isomer (5R, 4R), isomer (5R, 4S), and any combination thereof, isoxathiapiprolin may comprise a molar proportion of isomer (5S, 4R) greater than 50%, for example at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99%, relative to the total amount of isomers (5S, 4R), (5S, 4S), (5R, 4R) and (5R, 4S).

[0008] The molar proportion of the isomer (5S,4S) relative to the total amount of isomers (5S,4R), (5S,4S), (5R,4R) and (5R,4S) can be less than 50%, for example, at most 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5% or 0.1%.

[0009] The molar proportion of the isomer (5R,4R) relative to the total amount of isomers (5S,4R), (5S,4S), (5R,4R) and (5R,4S) can be less than 50%, for example, at most 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5% or 0.1%.

[0010] The molar proportion of the isomer (5R,4S) relative to the total amount of isomers (5S,4R), (5S,4S), (5R,4R) and (5R,4S) can be less than 50%, for example, at most 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5% or 0.1%.

[0011] Component B is a component selected from Bacillus species.

[0012] Bacillus species are insecticidal and / or nematicidal bacteria and may include, for example, Bacillus firmus, Bacillus cereus, Bacillus subtilis, or Bacillus thuringiensis.

[0013] The most preferred Bacillus species is Bacillus thuringiensis, and more particularly Bacillus thuringiensis var. kurstaki or Bacillus thuringiensis var. tenebrionis.

[0014] For example, the Bacillus thuringiensis of the present invention may be COSTAR provided by Syngenta, or NOVODOR provided by Leu+Gygax or BioSciences.

[0015] In the present invention, the bacillus composition of the present invention can be commercially available or can be obtained by culturing bacillus strains (e.g., bacillus thuringiensis strains) or insecticidal mutants (strains) derived therefrom according to methods well known in the art. For example, conventional large-scale microbial culture methods include submerged fermentation, solid-state fermentation, or liquid surface culture. When fermentation is about to end, as nutrients are exhausted, cells begin to change from the growth phase to the sporulation phase, so that the final product of fermentation is mainly spores, metabolites, and residual fermentation medium. Spore formation is a part of the natural life cycle of bacillus thuringiensis, and is usually started by cells in response to nutrient restriction. Fermentation is configured to obtain high-level colony forming units and promote sporulation. The bacterial cells, spores, and metabolites in the culture medium produced by fermentation can be directly used or concentrated by conventional industrial methods (such as centrifugation, tangential flow filtration, deep filtration, and evaporation).

[0016] The term "fermentation" broadly refers to the enzymatic and anaerobic or aerobic decomposition of organic matter (e.g., carbon substrate) by microorganisms under controlled conditions (e.g., temperature, oxygen, pH, nutrients, etc.) to produce fermentation products. Although fermentation typically describes processes that occur under anaerobic conditions, as used herein, this is not intended to limit the term to strictly anaerobic conditions, as the term "fermentation" as used herein can also occur in processes that occur in the presence of oxygen.

[0017] The composition of the present invention comprises fermentation product. In some embodiments, the concentrated fermentation broth is washed, for example, via a diafiltration process to remove residual fermentation broth and metabolites. As used herein, the term "broth concentrate" refers to a whole broth (fermentation broth) that has been concentrated by conventional industrial methods as described above but still remains in liquid form. As used herein, the term "fermentation solids" refers to the solid material remaining after the fermentation broth is dried. As used herein, the term "fermentation product" refers to whole broth, broth concentrate and / or fermentation solids. The composition of the present invention comprises fermentation product.

[0018] In a typical embodiment, the fermentation product produced by the Bacillus thuringiensis species will also include insecticidal toxins, such as at least one of a crystalline (Cry) protein, a cytolytic (Cyt) protein, and a vegetative insecticidal protein (Vip). For example, Bacillus thuringiensis subsp. Kurstaki strain SA-12 contains genes encoding a potential cytotoxin K2 type (CytK2), six crystalline insecticidal proteins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ia, Cry2Aa, Cry2Ab), two Cry-like proteins, and a vegetative insecticidal protein (VIP 3Aa10).

[0019] The fermentation broth or broth concentrate can be dried using conventional drying processes or methods such as spray drying, freeze drying, tray drying, fluidized bed drying, drum drying, or evaporation, with or without the addition of a carrier.

[0020] The resulting dry product can be further processed, such as by milling or granulation, to achieve a specific particle size or physical form. A carrier can also be added after drying.

[0021] The cell-free preparation of the fermentation broth of the strain of the present invention can be obtained by any means known in the art (such as extraction, centrifugation and / or filtration of the fermentation broth). It will be understood by those skilled in the art that the so-called cell-free preparation may not be cell-free, but rather largely cell-free or substantially cell-free, depending on the technique used to remove the cells (e.g., the speed of centrifugation). The resulting cell-free preparation can be dried and / or formulated with components that facilitate its application to plants or plant growth media. The concentration methods and drying techniques described above for the fermentation broth are also applicable to the cell-free preparation.

[0022] In one embodiment, the fermentation product comprises at least about 1×10 4 Colony forming units (CFU) of a microorganism (e.g., a Bacillus thuringiensis strain or an insecticidal mutant thereof) / mL of broth. In another embodiment, the fermentation product comprises at least about 1×10 5 In another embodiment, the fermentation product comprises at least about 1×10 6 CFU of microorganisms / mL broth. In yet another embodiment, the fermentation product comprises at least about 1×10 7 CFU of microorganisms / mL broth. In another embodiment, the fermentation product comprises at least about 1×10 8 CFU of microorganisms / mL broth. In another embodiment, the fermentation product comprises at least about 1×10 9 CFU of microorganisms / mL broth. In another embodiment, the fermentation product comprises at least about 1×10 19 CFU of microorganisms / mL broth. In another embodiment, the fermentation product comprises at least about 1×10 11 CFU of microorganisms / mL broth.

[0023] "Mutant" refers to a bacterial species with an alteration or variation (e.g., in a nucleotide sequence or amino acid sequence) that results in the organism and / or sequence being different from the naturally occurring or wild-type organism to which the mutant is compared. In some embodiments, the alteration or variation may be one or more nucleotide and / or amino acid substitutions or modifications (e.g., deletions or additions). In some embodiments, one or more amino acid substitutions or modifications may be conservative; herein, such conservative amino acid substitutions and / or modifications in a "mutant" do not substantially reduce the activity of the mutant relative to its non-mutant form. In some instances, the mutation may, for example, render the Bacillus species auxotrophic.

[0024] According to the present invention, components (A) and (B) are present in the composition in synergistically effective amounts.

[0025] More specifically, the weight ratio in grams of the active ingredients of component A to component B in the composition may be in the range of 1:2000 to 1:2, preferably 1:1000 to 1:10, preferably 1:1000 to 1:100, preferably 1:400 to 1:100, and more preferably 1:200 to 1:100.

[0026] Component A according to the present invention can be used to prepare suspension concentrates (SC), flowable suspensions (FS), suspoemulsions (SE), suspension concentrate-capsule suspension blends (ZC), water-soluble granules (SG), water-dispersible granules (WG), water-dispersible tablets (WT), dispersible concentrates (DC), or oil dispersions (OD). The most preferred formulation types are suspension concentrates, flowable suspensions or dispersible concentrates.

[0027] The above-described formulations can be prepared using ingredients and techniques well known in the art.

[0028] For example, the pesticidal composition according to the present invention, especially for preparing component A, may further comprise at least one of the components selected from polyoxyalkylene copolymers, acrylic graft copolymers, oxygen-containing hydrocarbons, lignin compounds, lactamide compounds, and ether solvents, preferably at least two of the components, preferably at least three of the components, and more preferably at least four of the components.

[0029] In a specific embodiment of forming a suspension concentrate, the pesticidal composition, especially for preparing component A, may further comprise at least one component selected from polyoxyalkylene copolymers, acrylic graft copolymers, and oxygen-containing hydrocarbons, preferably at least two of the components, and more preferably the three components.

[0030] In a specific embodiment of forming a dispersible concentrate, the pesticidal composition, especially for preparing component A, may further comprise at least one of the components selected from the group consisting of an acrylic graft copolymer, a lignin compound, a lactamide compound and an ether solvent, preferably at least two of the components, preferably at least three of the components, and more preferably the four components.

[0031] In the present invention, the polyoxyalkylene copolymer may be obtained from at least two different alkylene oxides, such as from ethylene oxide monomers and propylene oxide monomers.

[0032] The polyoxyalkylene copolymer may more preferably be an AB, ABA, BAB, or ABABA type polyoxyalkylene block copolymer.

[0033] More specifically, the polyoxyalkylene copolymers can be prepared by ring-opening polymerization of corresponding cyclic ethylene oxide monomers and propylene oxide monomers.

[0034] Typically, the ring-opening polymerization is initiated by the addition of water and alkali metal hydroxides such as sodium hydroxide and potassium hydroxide.The block structure of the copolymer is formed by first polymerizing a polymer block using one monomer and then adding a second monomer to form additional polymer blocks.

[0035] In a preferred embodiment, the polyoxyalkylene copolymer can be an ethylene oxide-propylene oxide-ethylene oxide block copolymer (EO-PO-EO block copolymer), or in other words a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymer or a poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol) block copolymer.

[0036] The polyoxyalkylene copolymer of the present invention, and more particularly the EO-PO-EO block copolymer, may have a molecular weight of 1,000 g / mol to 15,000 g / mol, and more preferably 3,000 g / mol to 7,000 g / mol.

[0037] In the present invention, the expression "molecular weight" means the average molecular weight (ie the approximate molecular weight). The molecular weight, or in other words the molar mass, of a polymer can be easily determined by methods well known in the art, such as gel permeation chromatography (GPC).

[0038] In a first embodiment, the EO-PO-EO block copolymer may have a polypropylene oxide molecular weight of 900 g / mol to 4,000 g / mol, and preferably 2,000 g / mol to 4,000 g / mol. In other words, the polypropylene oxide molecular weight is the molecular weight of the poly(propylene oxide) block of the EO-PO-EO block copolymer.

[0039] In a second embodiment, the EO-PO-EO block copolymer may have an ethylene oxide content of 10% to 80%, and preferably 30% to 50%, relative to the total weight of the copolymer. In other words, the ethylene oxide content is the percentage of poly(ethylene oxide) blocks in the EO-PO-EO block copolymer.

[0040] In a third embodiment, the EO-PO-EO block copolymer may have the features of the first embodiment and the features of the second embodiment.

[0041] Examples include PF series (CLARIANT), Series (BASF), PE series (CRODA), or Series (STEPAN).

[0042] The acrylic graft copolymer typically has a comb-like or star-like structure, and preferably a comb-like structure.

[0043] A graft copolymer is a branched copolymer in which the components forming the side chains are structurally different from the components forming the main chain.

[0044] Comb polymers comprise a main chain (backbone) containing branching points, from each of which emanate linear side chains.

[0045] Star polymers contain a multifunctional center from which at least three polymer chains radiate.

[0046] In a preferred embodiment, the acrylic graft copolymer may be an amphiphilic copolymer.

[0047] More particularly, the acrylic graft copolymer comprises at least one component A (hydrophilic part), which is soluble by the aqueous medium, and at least one other component B, which is hydrophobic.

[0048] Suitable acrylic graft copolymers may include polyethylene glycol, monomethyl ether of polyethylene glycol, poly(vinyl pyrrolidone), poly(acrylamide), or poly(vinyl alcohol) as hydrophilic side chains, while the hydrophobic backbone may include polymers and copolymers of styrene, methyl acrylate, methyl methacrylate, ethyl acrylate, 2-ethylhexyl acrylate, lauryl methacrylate, or vinyl acetate.

[0049] Such acrylic graft copolymers can be prepared, for example, by converting the monomethyl ether of polyethylene glycol into an acrylate or methacrylate, which is then subjected to free radical polymerization together with other unsaturated monomers such as styrene, ethyl acrylate, or methyl methacrylate. It is also possible to prepare such acrylic graft copolymers by reacting a hydrophobic polymer backbone consisting of chemically reactive sites such as carboxyl, hydroxyl, or amine groups with monomeric alkylene oxides such as ethylene oxide and propylene oxide to form hydrophilic side chains.

[0050] More preferably, the acrylic graft copolymer is a nonionic polymer and more particularly has a comb-like structure.

[0051] In the present invention, the acrylic graft copolymer may contain polyethylene glycol and / or monoether polyethylene glycol side chains.

[0052] The acrylic graft copolymer may also comprise a backbone derived from acrylate and / or methacrylate monomers.

[0053] Even more preferably, the acrylic graft copolymer may comprise a backbone obtained from acrylate and / or methacrylate monomers and side chains comprising polyethylene glycol and / or monoether polyethylene glycol, so as to more particularly impart a comb-like structure to the polymer.

[0054] For example, the acrylic acid graft copolymer of the present invention can be Atlox 4913 provided by Croda Corporation. TM , or Tersperse2500 provided by Huntsman TM .

[0055] The oxygen-containing hydrocarbon compound may be selected from alkyl ether compounds, alkyl ester compounds, and any mixture thereof; and is preferably selected from polyethylene glycol alkyl ethers, sulfonated alkyl ester compounds, and any mixture thereof. In other words, the oxygen-containing hydrocarbon compound is preferably different from the polyoxyalkylene copolymer and different from the acrylic graft copolymer.

[0056] Polyethylene glycol alkyl ethers can be obtained by reacting alcohols with ethylene oxide. Suitable alcohols are C2 to C 15 The chain length is preferably C7 to C 11 chain length, and more preferably has C9 or C 10 An example of a linear or branched fatty alcohol is Rhodasurf DA / 630-E provided by Solvay-Rhodia. TM ,

[0057] The sulfonated alkyl ester compound may be a sulfosuccinate or a salt of a sulfosuccinate. Examples may be Aerosol supplied by Solvay-Rhodia. TM OT-B.

[0058] The lignin compound may have a molecular weight of up to 10000 g / mol, and preferably up to 5000 g / mol. In a preferred embodiment, the lignin compound may have a molecular weight of at least 1000 g / mol.

[0059] The lignin compound may more particularly be kraft lignin and / or unsulfonated lignin. In a preferred embodiment, the lignin compound may be unsulfonated kraft lignin.

[0060] Unsulfonated kraft lignin can be defined as lignin separated from cellulose using the kraft process (also known as the kraft process), i.e., separated using sodium hydroxide (NaOH) and sodium sulfide (Na2S). Unlike lignin produced via the acid sulfite process, unsulfonated lignin is produced using this process.

[0061] The lactamide compound may have the formula I: CH3CH(OH)C(=O)NR1R2(I), wherein R1 and R2 are each independently hydrogen; or C 1-6 Alkyl, C 2-6 Alkenyl or C 3-6 cycloalkyl, each of which is optionally substituted by up to three independently selected groups consisting of phenyl, hydroxy, C 1-5 Alkoxy, morpholinyl and NR3R4 (wherein R3 and R4 are each independently C 1-3 alkyl) substituent; or phenyl, which is optionally substituted by up to three independently selected from C 1-3or R1 and R2 together with the nitrogen atom to which they are attached form a morpholinyl, pyrrolidinyl, piperidinyl or azepanyl ring, each of which is optionally substituted by up to three independently selected C 1-3 In a preferred embodiment, R1 and R2 of the lactamide compound of formula I can each independently be hydrogen or C 1-6 alkyl, and more preferably hydrogen or C 1-3 Alkyl groups, such as lactic acid dimethylamide or lactic acid diethylamide.

[0062] The ether solvent can be selected from dialkyl ether, dialkylamide ether, glycol ether, aliphatic ether, and cyclic ether. For example, the ether solvent can be 5-(dimethylamino)-2-methyl-5-oxopentanoic acid methyl ester, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monophenyl ether, tetrahydrofuran or dibutyl ether.

[0063] In the present invention, the composition may further contain water.

[0064] The composition of the present invention may further comprise one or more formulation additives well known in the art. In particular, the formulation additives may be selected from antifreeze agents, defoamers, antimicrobial agents (or biocides), viscosity regulators (or thickeners), pH regulators, and any mixtures thereof.

[0065] In a preferred embodiment, the composition of the present invention may further comprise at least one silicone compound as a defoaming agent. An example of the silicone compound may be polydimethylsiloxane.

[0066] Other suitable formulation additives include antioxidants, emulsifiers, colorants (or pigments), fragrances, adjuvants, attractants, adhesives, buffers, solid supports (carriers), coating agents, deodorants, emetics, inorganic fillers, safeners, organic solvents, light protectants, and any mixtures thereof, in addition to other additives known to those skilled in the art.

[0067] The composition of the present invention may further comprise additional agricultural chemicals. The additional agricultural chemicals may be insecticides, fungicides, herbicides, synergists, plant growth regulators, nematicides, plant nutrients, plant fertilizers, and mixtures thereof.

[0068] The composition of the present invention may contain one or more of the above-mentioned one or more components in appropriate amounts to obtain corresponding properties as appropriate.

[0069] In certain embodiments, when component A is prepared to form a formulation (such as a suspension concentrate, a flowable suspension, a suspoemulsion, a suspension concentrate-capsule suspension blend, a water-soluble granule, a water-dispersible granule, a water-dispersible tablet, a dispersible concentrate, or an oil dispersion), the formulation may include:

[0070] 0.01 to 50.0% by weight of component A, and preferably 0.1 to 40.0% by weight of component A, relative to the total weight of the formulation,

[0071] - polyoxyalkylene copolymers, preferably from 0.0005% to 50.0% by weight of polyoxyalkylene copolymers and more preferably from 0.002% to 25.0% by weight of polyoxyalkylene copolymers relative to the total weight of the formulation,

[0072] - acrylic acid graft copolymers, preferably 0.0005% to 20.0% by weight of acrylic acid graft polymers, and more preferably 0.001% to 10.0% by weight of acrylic acid graft polymers relative to the total weight of the formulation,

[0073] - oxygenated hydrocarbon compounds, preferably from 0.0001% to 10.0% by weight of oxygenated hydrocarbon compounds, and more preferably from 0.001% to 5.0% by weight of oxygenated hydrocarbon compounds relative to the total weight of the formulation, and

[0074] -Optionally water.

[0075] The above formulations are preferably formulated to form a suspension concentrate or a flowable suspension.

[0076] In certain embodiments, when component A is prepared to form a formulation (such as a suspension concentrate, a flowable suspension, a suspoemulsion, a suspension concentrate-capsule suspension blend, a water-soluble granule, a water-dispersible granule, a water-dispersible tablet, a dispersible concentrate, or an oil dispersion), the formulation may include:

[0077] (a) 0.01% to 70.0% by weight of component A, and preferably 0.1% to 50.0% by weight of component A, relative to the total weight of the formulation,

[0078] (b) lignin compounds, preferably 0.01% to 30.0% by weight of lignin compounds and preferably 0.1% to 20.0% by weight of lignin compounds relative to the total weight of the formulation,

[0079] (c) optionally an acrylic acid graft copolymer, preferably 0.01% to 30.0% by weight of an acrylic acid graft copolymer, and preferably 0.1% to 20.0% by weight of an acrylic acid graft copolymer relative to the total weight of the formulation,

[0080] (d) optionally a lactamide compound, preferably from 10.0% to 90.0% by weight of a lactamide compound, and preferably from 20% to 80% by weight of a lactamide compound relative to the total weight of the composition, and

[0081] (e) optionally an ether type solvent, preferably 1.0 to 70.0% by weight of an ether type solvent, and preferably 1.0 to 60.0% by weight of an ether type solvent relative to the total weight of the composition.

[0082] The above formulations are preferably formulated to form dispersible concentrates.

[0083] In the specific embodiment, isoxathiapiprole, polyoxyalkylene copolymers, acrylic acid graft copolymers and oxygen-containing hydrocarbons are compounds described in the present specification.

[0084] Component B according to the invention can be used for the preparation of water-dispersible granules (WG) or wettable powders (WP).

[0085] The above-described formulations can be prepared using ingredients and techniques well known in the art.

[0086] For example, the water-dispersible granules containing component B may be the product provided by Syngenta WG.

[0087] The pesticidal composition according to the present invention, especially the pesticidal composition for preparing component B, may further comprise at least one sulfonated compound, preferably a polyorganosulfonate, and more preferably sodium poly(naphthaldehyde)sulfonate.

[0088] In certain embodiments, when component B is prepared to form a formulation (such as a water-dispersible granule or a wettable powder), the formulation may comprise:

[0089] - 1.0 to 40% by weight of component B, and preferably 10 to 30% by weight of component B, relative to the total weight of the formulation, and

[0090] - 20 to 60% by weight of sulfonated compound relative to the total weight of the formulation.

[0091] In a preferred embodiment, according to the present invention, the pesticidal composition may be a mixture of component A and component B, and more particularly a mixture of:

[0092] a formulation containing component A, such as a suspension concentrate, a flowable suspension, a suspoemulsion, a suspension concentrate-capsule suspension blend, a water-soluble granule, a water-dispersible granule, a water-dispersible tablet, a dispersible concentrate, or an oil dispersion, and

[0093] - Formulations containing component B, such as water-dispersible granules or wettable powders.

[0094] The mixture can be diluted with water in a tank mixer.

[0095] A formulation containing component A and a formulation containing component B can be added to a farmer's water spray tank, or once mixed it can be applied directly without further dilution.

[0096] In further embodiments, component A and component B may be administered separately in any desired order or simultaneously.

[0097] In further embodiments, the formulation containing component A and the formulation containing component B can be applied separately in any desired order or simultaneously.

[0098] Another object of the present invention relates to a method for combating and / or controlling animal pests, which comprises applying a combination of components A and B to the pests, the locations of the pests, the plants susceptible to pests, or the plant propagation materials susceptible to pests, characterized in that components A and B are as defined in the present invention.

[0099] In other words, the combination is a pesticidal combination comprising component A and component B as defined in the present invention, wherein component A is isoxathiapiprolin and component B is a component selected from Bacillus species, fermentation solids thereof, and insecticidal toxins thereof. The combination can be applied in any desired order or simultaneously.

[0100] The combination of components A and B may be a pesticidal composition according to the invention.

[0101] In the method according to the invention, the composition can generally be applied together with an effective amount of isoxathiapiprolin and an effective amount of Bacillus species. More particularly, the weight ratio of component A to component B in the composition can be in the range of 1:1 to 1:10000, preferably 1:1 to 1:2000, preferably 1:1 to 1:1000, preferably 1:1 to 1:500, preferably 1:1 to 1:400, preferably 1:1 to 1:300, more preferably 1:1 to 1:200, more preferably 1:1 to 1:100, more preferably 1:1 to 1:10, and more preferably 1:2 to 1:10.

[0102] More particularly, for foliar application, the weight ratio of component A to component B in the composition may be in the range of 1:100 to 1:120, and preferably 1:110 to 1:115, such as, for example, 1:112.5.

[0103] For seed treatment applications, the weight ratio of component A to component B in the composition may range from 1:2 to 1:20, preferably 1:3 to 1:10, and more preferably 1:5 to 1:10.

[0104] The composition may be a mixture of component A formulated as described in the present invention (ie a formulation containing component A) and component B formulated as described in the present invention (ie a formulation containing component B). Water may optionally be added to the mixture.

[0105] The animal pests may be invertebrate pests, such as insects and mites. The pests may more preferably be selected from the order Lepidoptera and Coleoptera.

[0106] The Lepidoptera may be from the Noctuidae family (e.g. such as Spodoptera sp., Helicoverpa sp., Trichoplusia sp., Chrysodeixis sp., Autographa sp.) and the Gelechiidae family (e.g. such as Tuta sp., Anarsia sp., Phthorimaea sp.). The most preferred pests may be from the Spodoptera genus (e.g. such as Spodoptera littoralis), Tuta genus (e.g. such as Tuta absoluta), or Helicoverpa genus (e.g. such as Helicoverpa armigera).

[0107] The order Coleoptera may be selected from the family of the Beetles (e.g., species of the genera Beetles, species of the genera Ctenoceros, species of the genera Tenesera, species of the genera Chrysophypnus, species of the genera Short, species of the genera Wireworm, species of the genera Chrysophypnus, species of the genera Short, species of the genera Wireworm, species of the genera Hadromorphus, species of the genera Short, species of the genera Comb, species of the genera Pheletes, species of the genera Broad-breasted, species of the genera Wireworm, species of the genera Pheletes, species of the genera Grooved, species of the genera Sugarcane Wireworm, Pheletes californicus, aeripennistinctus Ctenoceros, aeripennis destructor Ctenoceros, potato beetles, aeripennisdestructor, golden destructor, golden aeripennis destructor, golden aeripennis aeripennis, Chyptohypnus nocturnus bicolor, Cryptohypnus nocturnus (Eschscholtz), nocturnus short beetle, two-color beetle, beet beetle, wireworm, wheat wireworm, Hadromorphus glaucus, short-breasted beetle, rufipalpis beetle, proximus beetle, littigiosus beetle, criddlei beetle, straight-striped beetle, dark-colored beetle, field beetle, European beetle, burnt brown short-breasted beetle, abbreviates short beetle, gray-headed beetle, pctoralis beetle, smoky brown beetle, slender-breasted beetle, opacicollis leconte), Tenebriophyidae (e.g., G. leconte, Adelium species, Pterohelaeus species, G. coarse-striped, G. diplotene, G. carpentariae (northern ground beetle), G. contractum, G. dorsogranosum, G. elderi (vegetable beetle), G. holmii, G. misellum, G. paleuille, G. mongolica, G. rusticum, G. seriatum, G. setulosum, G. torridum, G. tuberculosis, G.The most preferred pests may be from the Diabrotica genus (e.g., Diabrotica balteata), or from the Agriotes genus (e.g., Agriotes lineatus). .

[0108] The composition according to the invention can be used to combat and / or control (i.e. contain or destroy) harmful organisms of the above-mentioned type, which occur in particular on plants, especially on useful plants and ornamental plants in agriculture, in horticulture and in forestry, or on organs of these plants, such as fruits, flowers, leaves, stems, tubers or roots, and in some cases, even plant organs formed at a subsequent point in time remain protected against these harmful organisms. The composition can also be used to combat and / or control (i.e. contain or destroy) harmful organisms of the above-mentioned type, which occur in particular in the soil and / or on plant propagation materials.

[0109] The term "plant" as used in this specification includes seedlings, shrubs and trees. In particular, suitable target crops are cereals, such as wheat, barley, rye, oats, rice, corn (e.g., field corn, popcorn corn, corn), millet or sorghum; beets, such as sugar beets or fodder beets; fruits, such as pome, drupe or soft fruit, such as apples, pears, plums, peaches, apricots, cherries or berries (e.g., strawberries, raspberries or blackberries); leguminous crops, such as beans, lentils, peas or soybeans (soya / soyabeans); oilseed crops, such as rapeseed, mustard, poppy, olive, sunflower seeds, etc. sunflower, coconut, castor, cocoa beans, or peanuts; melons, such as pumpkin, cucumber, melon, watermelon, or squash; fiber plants, such as cotton, flax, hemp, or jute; citrus fruits, such as oranges, lemons, grapefruits, or tangerines; vegetables, such as spinach, lettuce, asparagus, cabbage, broccoli, cauliflower, carrots, onions, tomatoes, potatoes, eggplant, peppers, or bell peppers; lauraceae, such as avocado, cinnamon, or camphor; and also tobacco, nuts, coffee, sugar cane, tea, grapevines, hops, the plant family, and latex plants.

[0110] In a preferred embodiment, the plant may be selected from:

[0111] - Cereals, such as wheat, barley, rye, oats, rice, corn (e.g., field corn, popcorn, maize), millet, or sorghum;

[0112] - Leguminous crops such as beans, lentils, peas or soya (soya / soya beans);

[0113] - fiber plants such as cotton, flax, hemp or jute; and

[0114] -Vegetables, such as spinach, lettuce, asparagus, cabbage, broccoli, cauliflower, carrots, onions, tomatoes, potatoes, and peppers or bell peppers.

[0115] The term "plant propagation material" is understood to mean all reproductive parts of a plant, such as seeds, which can be used for the latter's proliferation, including vegetative plant materials, such as cuttings. Seeds (strictly speaking), roots, fruits, tubers, bulbs, rhizomes, parts of plants can be mentioned as plant propagation materials. Before sowing or planting the plant propagation material, the material can be treated with the composition of the present invention. Alternatively, during sowing or planting, the plant propagation material can be treated with the composition of the present invention. In addition, before or during the planting of plant propagation material, the composition of the present invention can be applied to the previously treated plant propagation material. The composition of the present invention can be applied during the sowing of seeds. The composition can also be used for plant propagation materials derived from plants grown in greenhouses and / or during transplanting.

[0116] More preferably, plant propagation material is plant seed.For the purpose of germination and growth of plant, seed treatment can occur in unsown seed, and term " unsown seed " means to include the seed in any period between the harvest of seed and the sowing of seed in the soil.For the processing of unsown seed, it is not intended to include that wherein said composition is applied to those practices in the soil but to include any application practice that seed is targeted during the sowing / planting process.The treated plant propagation material of the present invention can be processed in the same manner as conventional plant propagation material.These treated plant propagation materials can be stored, processed, sown and cultivated in the same manner as the material processed by any other pesticide.

[0117] In a first specific embodiment, the pesticidal composition according to the present invention can be applied to pests, the location of pests, or plants susceptible to pests by spraying the pesticidal composition (e.g., dispensing from a spray container for foliar application). The method may involve applying an effective amount of the pesticidal composition at the following rate:

[0118] - 0.01 g to 200 g of component A per hectare, preferably 0.1 g to 100 g of component A per hectare, and more preferably 50 g to 100 g of component A per hectare; and

[0119] - 0.1 g to 1000 g of component B per hectare, preferably 1 g to 500 g of component B per hectare, and more preferably 100 g to 500 g of component B per hectare.

[0120] More particularly, for foliar application, the weight ratio of component B is at least 10 times the weight ratio of component A, preferably at least 50 times the weight ratio of component A, and more preferably at least 100 times the weight ratio of component A. Preferably, the weight ratio of component B is at most 200 times the weight ratio of component A. In a second specific embodiment, the pesticidal composition according to the present invention can be applied to a plant propagation material susceptible to pests. In this case, the pesticidal composition can be used as a seed treatment. The method may involve applying an effective amount of the pesticidal composition at the following ratio:

[0121] 0.01 g to 30 g of component A per 100 kg of seeds, preferably 0.01 g to 25 g of component A per 100 kg of seeds, preferably 0.1 g to 25 g of component A per 100 kg of seeds, preferably 0.1 g to 10 g of component A per 100 kg of seeds, preferably 0.1 g to 5 g of component A per 100 kg of seeds, and more preferably 0.5 g to 1 g of component A per 100 kg of seeds, and

[0122] 1 g to 1000 g of component B per 100 kg of seeds, preferably 5 g to 500 g of component B per 100 kg of seeds, preferably 10 g to 200 g of component B per 100 kg of seeds, preferably 10 g to 150 g of component B per 100 kg of seeds, and more preferably 50 g to 100 g of component B per 100 kg of seeds.

[0123] More particularly, for seed treatment applications, the weight ratio of component B is at least 2 times the weight ratio of component A, preferably at least 2.5 times the weight ratio of component A, preferably at least 3 times the weight ratio of component A, more preferably 5 times the weight ratio of component A,

[0124] And more preferably 10 times the weight ratio of component A. The weight ratio of component B may be at most 50 times the weight ratio of component A, preferably at most 40 times the weight ratio of component A, and more preferably at most 30 times the weight ratio of component A.

[0125] In another object, the pesticidal composition of the present invention can be used to combat and / or control the following pests:

[0126] from the order Lepidoptera, more preferably from the family Noctuidae (such as for example species of the genus Spodoptera, Spodoptera, Spodoptera, Goutioscelidae, Spodoptera) or from the family Lycopodiidae (such as for example species of the genus Leafminer, Gnaphalina, Gnaphalina), and / or

[0127] from the order Coleoptera, more preferably from the order Coleoptera which may be selected from the family of the Beetles (such as for example species of the genus Beetles, species of the genus Ctenoceros, species of the genus Tenesera, species of the genus Chyptohypnus, species of the genus Short-beetles, species of the genus Chrysophora, species of the genus Chyptohypnus, species of the genus Short-beetles, species of the genus Chrysophora, species of the family Hadromorphus, species of the genus Short-beetles, species of the genus Comb-clawed beetles, species of the genus Pheletes, species of the genus Broad-breasted beetles, species of the genus Chrysophora, species of the genus Grooved beetles, species of the family Chrysophora, Pheletescalifornicus, aeripennis tinctus, aeripennis destructor, potato beetles, aeripennis destructor, destructor golden beetles, aeripennis destructor golden beetles, aeripennis aeripennis golden beetles, Chyptohypnus nocturnus bicolor, Cryptohypnusnocturnus (Eschscholtz), nocturnus short beetle, two-color beetle, beet beetle, wireworm, wheat wireworm, Hadromorphus glaucus, short beetle, rufipalpis beetle, proximus beetle, littigiosus beetle, criddlei beetle, straight beetle, dark beetle, field beetle, European beetle, burnt brown short beetle, abbreviates short beetle, gray-headed beetle, pctoralis beetle, smoky brown beetle, slender-breasted beetle, opacicollis leconte), Tenebriophyidae (e.g., G. leconte, Adelium species, Pterohelaeus species, G. coarse-striped, G. diplotene, G. carpentariae (northern ground beetle), G. contractum, G. dorsogranosum, G. elderi (vegetable beetle), G. holmii, G. misellum, G. paleuille, G. mongolica, G. rusticum, G. seriatum, G. setulosum, G. torridum, G. tuberculosis, G.walker, Adelium brevicorne, Pterohelaeusalternatus), Chrysomelidae (e.g., species of root beetles, species of flower beetles, species of striped flea beetles, species of concave shanks), Scarabidae (e.g., species of horn beetles, species of square beetles, species of arc beetles, species of tooth claw beetles), Curculionidae (e.g., species of flower beetles, species of sharp cryptobeak beetles, species of ear beetles), and Cercopithecidae (e.g., species of cauliflower beetles). The most preferred pests may be from the genus root beetles (e.g., species of cucumber leaf beetles), or from the genus snap beetles (e.g., species of straight striped snap beetles). .

[0128] The active ingredient compositions according to the invention achieve a high degree of pest control in particular within a very short period of time, such as, for example, at least three days after application.

[0129] In the present invention, additional active ingredients can be combined with component A and / or component B of the present invention, and used in the method of the present invention, and applied simultaneously or sequentially with components A and B of the present invention. When applied simultaneously, these additional active ingredients can be formulated or mixed in, for example, a spray barrel together with the composition of the present invention. These additional biocidal active ingredients can be fungicides, herbicides, insecticides, bactericides, acaricides, nematicides, plant growth regulators and / or biological preparations.

[0130] The following mixtures of component A and / or component B with further active ingredients are preferred: acibenzolar-s-methyl, metalaxyl-M, metalaxyl, piperacillin, thiabendazole, ethaboxanil, fludioxonil, azoxystrobin, thiabendazole, difenoconazole, tebuconazole, ipconazole, clofoconazole, prothioconazole, trichlorfon, sedaxane, inpyrfluxam, penflufen, fluopyram, fluopyram, pydiflumetofen, cylobutrifluramide, m), thiamethoxam, isoflurane, chlothianidin, benzovinflumazole, cymoxanil, phosphites (phosphonates), silthiopyrad, pyraclostrobin, isothiopyrad, tolprocarb, quinofumelin, ipflufenoquin, metyltetraprole, florylpicoxamid, fenpicoxamid, fluopyram fluoxytioconazole, fluindapyr, isoflurane, fluoxapiprolin, broflanilide, imidacloprid, acetamiprid, cyantraniliprole, chlorantraniliprole, flucyantraniliprole, fluoxoxaprozil, penthiopyrad, dimethomorph, dimpropyridaz, flupyrimin, triflumezopyrim, oxazosulfonyl oxazosulfyl, dichlobentiazox, benzpyrimoxan, oxamexyl, pyrapropoyne, cyclobromofenamide, mandestrobin, copper, lambdha-cyhalothrin, cyproconazole, avermectin, tefluthrin, picoxystrobin, spiropidion, oligosaccharins, Melaleuca alternifoliaalternifolia, thiathioate, fenamifos, oxamyl, fluensulfone, fluazanindolizine, spirotetramat, spidoxamat, fluazinam, indazapyroxamet, thiophanate methyl, zoxamide, fluoxastrobin, pirimifos, tetrachlorantraniliprole, bifenthrin, and emamectin.

[0131] The following mixtures of component A and / or component B with further active ingredients are most preferred: acibenzolar-s-methyl, metalaxyl-M, metalaxyl, piperacillin, thiabendazole, ethaboxam, fludioxonil, azoxystrobin, thiabendazole, difenoconazole, tebuconazole, ipconazole, clofoconazole, prothioconazole, trichlorfon, fluopyram, indopyram, fluopyram, fluopyram, fluopyram, fluopyram, trifloxystrobin, triflupyramide, thiamethoxam, thiamethoxam.

[0132] In certain embodiments, Component A and / or Component B may be combined with at least two, three, or four of the additional active ingredients described above.

[0133] More particularly, component A and / or component B may be combined with at least two of the additional active ingredients selected from fluzoxacillin, mefenoxam, difenoconazole, thiamethoxam, and fludioxonil; preferably at least three of the additional active ingredients selected from fluzoxacillin, mefenoxam, difenoconazole, thiamethoxam, and fludioxonil; preferably at least four of the additional active ingredients selected from fluzoxacillin, mefenoxam, difenoconazole, thiamethoxam, and fludioxonil; and more preferably with all of the additional active ingredients selected from fluzoxacillin, mefenoxam, difenoconazole, thiamethoxam, and fludioxonil. A preferred combination may be a combination of component A with fluzoxacillin, mefenoxam, difenoconazole, and thiamethoxam, or a combination of component A with fluzoxacillin, mefenoxam, difenoconazole, and fludioxonil.

[0134] When component A, component B and at least one of the additional active ingredients are combined, or component A and at least two of the additional active ingredients are combined, or component B and at least two of the additional active ingredients are combined, the weight ratio between any two components may be independently of each other 1:100 to 100:1, preferably 1:50 to 50:1, and even more preferably 1:10 to 10:1. For example, the mixture can be as follows: component A (7.7 g / L), fluazifop (7.7 g / L), metalaxyl-M (15.3 g / L), difenoconazole (36.8 g / L), and thiamethoxam (61.3 g / L); component A (19.1 g / L), fluazifop (19.1 g / L), metalaxyl-M (38.4 g / L), difenoconazole (92.2 g / L), and thiamethoxam (153.4 g / L); or component A (15.4 g / L), fluazifop (15.4 g / L), metalaxyl-M (9.2 g / L), difenoconazole (36.9 g / L), and fludioxonil (7.7 g / L). Examples

[0135] The following non-limiting examples demonstrate the improved behavior associated with the pesticidal compositions according to the present invention.

[0136] A synergistic effect exists whenever the effect of the combination of active ingredients is greater than the sum of the effects of the individual components.

[0137] For a given combination of active ingredients, the expected effect E obeys the so-called COLBY formula and can be calculated as follows (COLBY, SR "Calculating synergistic and antagonistic responses of herbicide combination". Weeds, Vol. 15, pp. 20-22; 1967):

[0138] ppm = milligrams of active ingredient per litre of spray mixture

[0139] X = % effect based on active ingredient A) using p ppm of active ingredient

[0140] Y = % effect based on active ingredient B) using q ppm of active ingredient. According to Colby, using p+q ppm of active ingredient, the expected (additive) effect of active ingredient A) + B) is

[0141] If the effect actually observed (O) is greater than the expected effect (E), then the effect of the combination is superadditive, i.e. there is a synergistic effect. In mathematical terms, the synergy factor SF corresponds to O / E. In agricultural practice, an SF of ≥1.2 represents a significant improvement over the purely complementary addition of the activity (the expected activity), whereas an SF of ≤0.9 marks a loss of activity compared to the expected activity in actual application practices.

[0142] The products used in the following examples are detailed below:

[0143] - VIRANTRA SC400 (commercialized by Syngenta) is a SC400 formulation (suspension concentrate with 400 g of active ingredient per litre) comprising isoxathiapiprole as active ingredient;

[0144] - FS400 is a flowable concentrate for seed treatment containing 400 g of active ingredient per liter, which contains isoxathiapiprole as the active ingredient;

[0145] - COSTAR WG 18 (commercialized by Syngenta) is a WG product (water dispersible granules containing 18% active ingredient by weight relative to the total weight of the product) comprising 90.4 million IU / g of Bacillus thuringiensis subsp. Kurstaki strain SA-12 (Bt1) as active ingredient; and

[0146] - NOVODOR FC is a SC product (suspension concentrate containing 30 g / L active ingredient) which contains 10000 I.U. / mg of Bacillus thuringiensis subsp. pseudotrichiae strain NB-176 (Bt2) as active ingredient.

[0147] Experimental Description Example 1 (Spodoptera species)

[0148] The products VIRANTRA SC400 and COSTAR WG 18 were diluted with the addition of water to obtain the concentrations of active ingredients collected in Tables 1 and 2 and applied to soybean plants (planted in pots; about 8 plants per pot, 5 pots per treatment) by using a rotary disc applicator (500 l / ha). After drying, 10 soybean plants were cut and placed in a Petri dish (diameter: 14.5 cm) containing 3 wet filter papers (4 ml of water); 3 replicates were performed for each concentration. The plants were infested with 10 Spodoptera littoralis larvae (second instar larvae), and the Petri dish was covered with a round cotton paper towel and closed with a plastic cover. The mortality rate was assessed three and five days after application.

[0149] The results are collected in Tables 1 and 2 below, where Table 1 relates to the results three days after administration (3DAA) and Table 2 relates to the results five days after administration (5DAA).

[0150] [Table 1]

[0151] Table 2 Example 2 (root leaf beetle)

[0152] Products FS400 and NOVODOR 3% FC were diluted with water to obtain a dosage of active ingredient for 30 seeds plus an excess of 10%. These extra 10% are needed to compensate for the loss of active ingredient during the seed treatment process. 30 corn seeds were placed in a spherical glass beaker and the application solution was added. The beaker was shaken to mix the seeds with the solution. After drying, four seeds were sown in each pot. Five replications were prepared for each treatment. 14 days after sowing, these pots were infested with 5 second-instar larvae of the cucumber beetle. 6 days after infestation (6DAI), the mortality of the larvae and the damage to the plants were assessed.

[0153] The results are collected in the following tables, where Table 3 relates to the results of the mortality assessment 6 days after infestation and Table 4 relates to the assessment of plant damage 6 days after infestation (6 DAI).

[0154] [Table 3]

[0155] Table 4 Example 3 (Spp.)

[0156] Products FS400 and NOVODOR 3% FC are diluted with water to obtain the dosage of active ingredients for 30 seeds plus 10% excess. These extra 10% are needed to compensate for the loss of active ingredients during the seed treatment process. 30 barley seeds are placed in a spherical glass beaker and the application solution is added. The beaker is shaken to mix the seeds with the solution. After drying, two seeds are sown in each pot. Ten repetitions are prepared for each treatment. After 5 days of sowing, these pots are infested with 10 wireworms (straight bar beetles, about 20mm long). 20 days after infestation (20DAI), the corrected mortality of wireworms is evaluated based on the control treatment. 6 days after infestation (6DAI), plant height is measured.

[0157] The results are collected in the following tables, where Table 5 relates to the results of the mortality assessment 20 days after infestation (20 DAI) and Table 6 relates to the assessment of plant damage (plant height in cm) 6 days after infestation (6 DAI).

[0158] [Table 5]

[0159] Table 6

Claims

1. A pesticidal composition comprising component A and component B, characterized in that The component A is isoxathiapiprolin, and the component B is a component selected from Bacillus species, fermentation solids thereof, and insecticidal toxins thereof.

2. The pesticidal composition according to claim 1, characterized in that The component B is Bacillus thuringiensis, and preferably Bacillus thuringiensis subsp. Kurstaki or Bacillus thuringiensis subsp. Pseudomonas.

3. A pesticidal composition according to any one of the preceding claims, characterized in that The weight ratio of component A to component B may be in the range of 1:1 to 1:10000, preferably 1:1 to 1:2000, preferably 1:1 to 1:1000, and more preferably 1:1 to 1:

500.

4. A pesticidal composition according to any one of the preceding claims, characterised in that The component A is used to prepare a suspension concentrate, a flowable suspension, a suspoemulsion, a suspension concentrate-capsule suspension blend, a water-soluble granule, a water-dispersible granule, a water-dispersible tablet, a dispersible concentrate, or an oil dispersion.

5. A pesticidal composition according to any one of the preceding claims, characterised in that The component B is used to prepare water-dispersible granules or wettable powders.

6. A pesticidal composition according to any one of the preceding claims, characterised in that The composition further comprises at least one component selected from the group consisting of a polyoxyalkylene copolymer, an acrylic graft copolymer, an oxygen-containing hydrocarbon, a lignin compound, a lactamide compound, and an ether type solvent.

7. A pesticidal composition according to any one of the preceding claims, characterised in that The composition further comprises at least one sulfonated compound, preferably a polyorganosulfonate, and more preferably sodium poly(naphthaldehyde)sulfonate.

8. A pesticidal composition according to any one of the preceding claims, characterised in that The composition further comprises one or more formulation additives selected from the group consisting of an antifreeze agent, an antifoaming agent, an antibacterial agent (or biocide), a viscosity modifier, a pH modifier, and any mixture thereof.

9. A method for combating and / or controlling animal pests, the method comprising applying a combination of components A and B to the pests, the location of the pests, the plants susceptible to the pests, or the plant propagation materials susceptible to the pests, characterized in that The components A and B are as defined in any one of the preceding claims.

10. The method according to claim 9, characterized in that The components A and B are applied by mixing a formulation containing the component A and a formulation containing the component B.

11. The method according to claim 9 or 10, characterized in that The method is applied by spraying the pesticidal composition.

12. The method according to claim 9 or 10, wherein: The components A and B are applied in a sequential manner.

13. The method according to any one of claims 9 to 12, characterized in that The pests are from the order Lepidoptera and / or Coleoptera.

14. The method according to any one of claims 9 to 13, characterized in that The plant is selected from the group consisting of cereals, leguminous crops, fiber plants, and vegetables.

15. Use of a pesticidal composition according to any one of claims 1 to 8 for combating and / or controlling pests from the orders Lepidoptera and / or Coleoptera.

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