Traditional Chinese medicine composition and application thereof in preparation of product for preventing and treating recessive mastitis disease of sheep

Abstract

The invention discloses a traditional Chinese medicine composition and application thereof in preparation of products for preventing and treating recessive mastitis diseases of sheep. The traditional Chinese medicine composition is prepared from the following components: radix bupleuri, polygonum cuspidatum, dandelion, plantain herb, selfheal, astragalus membranaceus, honeysuckle stem and liquorice. The traditional Chinese medicine composition provided by the invention is good in palatability to ruminants and low in cost, and has a remarkable curative effect on sheep recessive mastitis caused by various reasons. The raw materials of the formula are natural and healthy, and the formula is suitable for modern animal husbandry and organic pasture.

Description

Technical Field

[0001] The invention relates to the field of veterinary medicine for livestock and poultry, and in particular to a Chinese medicine composition and a preparation method thereof and application of the composition in preparing a product for preventing and treating sheep latent mastitis. Background Art

[0002] This section merely provides background information related to the present disclosure and is not necessarily prior art.

[0003] As an important part of animal husbandry, dairy goats' dairy products are deeply loved by consumers because of their rich nutrition and easy digestion. However, in the process of dairy goat breeding, mastitis, as a common disease, poses a serious threat to the health of dairy goats and the quality of dairy products. Among them, invisible mastitis is often ignored because its symptoms are not obvious, but its long-term existence will lead to a decrease in milk production, deterioration of milk quality, and even cause more serious diseases, bringing immeasurable economic losses to farmers. Traditionally, chemical drugs such as antibiotics are mostly used to treat mastitis, but these drugs have problems such as drug resistance and drug residues, which not only affect the safety of dairy products, but also may pose a potential threat to the environment and human health. Therefore, it is particularly important to find a safe, effective and residue-free method for the prevention and treatment of mastitis. Summary of the invention

[0004] Purpose of the invention: The technical problem to be solved by the present invention is to provide a Chinese medicine composition for preventing and treating sheep latent mastitis and its preparation method and application in view of the deficiencies in the prior art.

[0005] In order to solve the above technical problems, the present invention discloses the following technical solutions:

[0006] In a first aspect, the present invention discloses a Chinese medicinal composition for preventing and treating latent mastitis in sheep, which is made from the following components: bupleurum, knotweed, dandelion, plantain, selfheal, astragalus, honeysuckle vine, and liquorice.

[0007] The pharmacological properties of each Chinese medicine component in the above Chinese medicine composition are as follows:

[0008] Bupleurum: Nature and flavor and meridians: bitter, slightly cold; enters the gallbladder and liver meridians; Functions and indications: releases exterior and harmonizes the interior, raises yang and soothes the liver; used for alternating cold and heat or spleen deficiency, etc.

[0009] Polygonum cuspidatum: Nature and flavor and meridians: bitter and cold; enters the gallbladder, liver and lung meridians; Functions and indications: promotes bile secretion and reduces jaundice, clears away heat and detoxifies, promotes blood circulation and removes blood stasis; used for damp-heat jaundice, stranguria and leucorrhea, etc.

[0010] Dandelion: Nature and flavor: bitter, sweet, and cold; it enters the liver and stomach meridians; functions and indications: clearing away heat and detoxifying, relieving swelling and dispersing blood stasis, and promoting diuresis and relieving stranguria; it is used for breast abscesses, carbuncles, furuncles, etc.

[0011] Plantago: Nature and flavor: sweet, light, and cold; it enters the liver, kidney, and lung meridians; functions and indications: diuresis and relieving stranguria, clearing away heat and detoxifying; it is used for heat-toxic carbuncles, damp-heat stranguria, etc.

[0012] Prunella Vulgaris: Nature and flavor: pungent, bitter, and cold; enters the liver and gallbladder meridians; Functions and indications: clears the liver and purges fire, disperses stagnation and reduces swelling; used for red and swollen eyes, mastitis, breast lumps, breast pain, etc.

[0013] Astragalus: Nature and flavor and meridians: sweet, warm; enters the lung and spleen meridians; Functions and indications: tonifies Qi and raises Yang, benefits the Wei and consolidates the exterior, promotes diuresis and reduces swelling, removes toxins and discharges pus, heals sores and promotes tissue regeneration; used for spleen and lung Qi deficiency, Qi and blood deficiency, etc.

[0014] Honeysuckle vine: Nature and flavor: sweet, cold; enters the lung and stomach meridians; Functions and indications: clears heat and detoxifies, soothes wind and dredges the meridians, used for fever caused by febrile diseases, carbuncles, ulcers, rheumatism and heat arthralgia, etc.

[0015] Licorice: Nature and flavor: sweet, flat; enters the heart, spleen, lung and kidney meridians; Functions and indications: tonifies the middle and replenishes qi, clears away heat and detoxifies, relieves pain; used for spleen and stomach weakness, sores, swelling and pain, etc.

[0016] The present invention is formulated according to the principle of syndrome differentiation and treatment of traditional Chinese veterinary medicine, and has been put into clinical practice for many times. Bupleurum, which can raise yang and lift the sunken, and Astragalus, which can replenish qi and support toxins and discharge pus, are the main medicines; Polygonum cuspidatum, which can clear away heat and detoxify, promote blood circulation and remove blood stasis, and Plantago, which can clear away heat and detoxify, promote diuresis and relieve stranguria, are the ministerial medicines; Taraxacum, Honeysuckle, which can clear away heat and detoxify, reduce swelling and disperse blood stasis, soothe wind and dredge collaterals, and Prunella vulgaris, which can disperse knots and reduce swelling, are the adjuvant medicines; Licorice, which can harmonize various medicines, is the guiding medicine. This prescription takes into account the hot constitution of sheep and various factors causing sheep mastitis, and at the same time takes into account the symptoms such as redness, swelling, heat and pain caused by mastitis, and adopts various medicines to be reasonably compatible, which complement each other and significantly improve the clinical effect.

[0017] In some embodiments, the weight proportions of each component are 50-70 parts of bupleurum, 40-60 parts of knotweed, 80-99 parts of dandelion, 50-70 parts of plantain, 35-55 parts of selfheal, 20-40 parts of astragalus, 20-40 parts of honeysuckle, and 35-55 parts of licorice. In some embodiments, the weight proportions of each component are 55-65 parts of bupleurum, 45-55 parts of knotweed, 85-95 parts of dandelion, 55-65 parts of plantain, 40-50 parts of selfheal, 25-35 parts of astragalus, 25-35 parts of honeysuckle, and 40-50 parts of licorice. In some embodiments, the weight proportions of each component are 60 parts of bupleurum, 50 parts of knotweed, 90 parts of dandelion, 60 parts of plantain, 45 parts of selfheal, 30 parts of astragalus, 30 parts of honeysuckle, and 45 parts of licorice.

[0018] In some embodiments, the traditional Chinese medicine composition is prepared by the following method: weighing each component according to the formula, crushing, countercurrent extraction, concentrating, and drying to obtain.

[0019] Wherein, the extraction solvent is drinking water.

[0020] The mass ratio of the solvent to the raw material is 10-20:1, preferably 15:1.

[0021] Wherein, the extraction temperature is 70-90°C, preferably 80°C.

[0022] Wherein, the concentration is to concentrate to a relative density of 1-1.4, preferably 1.2.

[0023] Wherein, the concentration is carried out by using a double-effect concentrator.

[0024] In some embodiments, the dosage form of the Chinese medicine composition is an oral dosage form, including but not limited to oral liquid, granules, powders, powders, pills, tablets or capsules. The preparation method of the oral drug dosage form is to use the Chinese medicine contained in the above formula as the active ingredient and add commonly used excipients or auxiliary ingredients to prepare an oral dosage form.

[0025] In a second aspect, the present invention discloses a method for preparing the Chinese medicine composition described in the first aspect, comprising weighing each component according to the formula, crushing, countercurrent extraction, concentrating, and drying to obtain the composition.

[0026] Wherein, the extraction solvent is drinking water.

[0027] The mass ratio of the solvent to the raw material is 10-20:1, preferably 15:1.

[0028] Wherein, the extraction temperature is 70-90°C, preferably 80°C.

[0029] Wherein, the concentration is to concentrate to a relative density of 1-1.4, preferably 1.2.

[0030] Wherein, the concentration is carried out by using a double-effect concentrator.

[0031] In a third aspect, the present invention discloses the use of the Chinese medicine composition described in the first aspect in the preparation of a product for preventing and treating sheep latent mastitis.

[0032] In a fourth aspect, the present invention discloses a product for preventing and treating sheep latent mastitis, which comprises the Chinese medicine composition described in the first aspect.

[0033] In the third and fourth aspects above,

[0034] The Chinese medicine composition can be fed directly, added to water, or added to feed.

[0035] The dosage of the Chinese medicine composition is calculated at 0.03-0.08 g / kg body weight per day, and is used continuously for 5-7 days.

[0036] When the application is to prevent sheep latent mastitis, such as postpartum preventive addition to dairy goats, the Chinese medicine composition is directly fed every day, with a dosage of 0.03-0.08 g / kg body weight, which can be taken in divided doses or all at once; for prevention, the dosage can be halved and used continuously for 7 days.

[0037] Wherein, the dosage is preferably 0.05 g / kg body weight.

[0038] The prevention and treatment mentioned in the present invention includes prophylaxis and / or treatment.

[0039] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0040] (1) The Chinese medicine composition provided by the present invention has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and reducing swelling and relieving pain; it is safe, has no side effects, and treats both the symptoms and the root causes. It is a Chinese medicine formula specially developed for sheep mastitis and has better effects than traditional Chinese medicine formulas.

[0041] (2) The Chinese medicine composition provided by the present invention has significant therapeutic effects. It can not only control the development of mastitis in sheep, but also quickly reduce fever, relieve symptoms, and improve the milk production and disease resistance of sick sheep.

[0042] (3) The Chinese medicine composition provided by the present invention is safe, has significant efficacy, low cost, fast fever reduction, good efficiency, good palatability, and is easy to use. It is a Chinese medicine formula for the prevention and treatment of sheep latent mastitis.

[0043] (4) The Chinese medicine composition provided by the present invention has good palatability for ruminants, and sheep can actively eat or drink water, which reduces the difficulty and workload of drug administration.

[0044] (5) The Chinese medicine composition provided by the present invention has the effects of soothing the liver and regulating qi, strengthening the spleen and harmonizing the stomach. When sheep develop latent mastitis, it can relieve symptoms and increase the feed intake and disease resistance of sick sheep, thereby improving the cure rate and reducing the incidence rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0046] Figure 1 These are the sheep with latent mastitis in Example 2.

[0047] Figure 2 The sheep with latent mastitis in Example 2 were gavaged with the Chinese medicinal compound.

[0048] Figure 3 This is a routine blood test of the sheep in Example 2.

[0049] Figure 4This is a diagnostic test for latent mastitis in sheep in Example 2. DETAILED DESCRIPTION

[0050] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0051] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0052] Example 1 Preparation of Chinese medicinal compound

[0053] Recipe: 60kg of Bupleurum, 50kg of Polygonum cuspidatum, 90kg of Taraxacum, 60kg of Plantain, 45kg of Prunella Vulgaris, 30kg of Astragalus, 30kg of Honeysuckle, and 45kg of Licorice.

[0054] Preparation method: Weigh the above medicinal materials according to weight ratio, screen and wash them; crush the medicinal materials until they pass through a 20-mesh screen; extract them by dynamic countercurrent reflux extraction method, the extraction solvent is drinking water, the mass ratio of the solvent to the raw material is 15:1, the heating temperature is 80°C, and the extraction time is 2.5h; concentrate the above extract to a relative density of 1.2. The concentration method uses a double-effect concentrator, and the vacuum degree and evaporation temperature of each effect are: one effect -0.05MPa, 85°C; the second effect -0.08MPa, 70°C, and the extract is spray-dried to obtain.

[0055] Example 2

[0056] 1 Materials and methods

[0057] 1.1 Experimental animals

[0058] The experiment selected 20 healthy dairy goats weighing 41.03±6.24kg, aged 1-2 years, and 1-2 parities in lactation, and 40 dairy goats with invisible mastitis detected by CMT method. Before and during the experiment, they were free to drink water, uniformly fed TMR, and had no other diseases and did not receive antibiotic treatment. Figure 1-Figure 4 shown.

[0059] The test period was 7 days and was divided into 3 groups:

[0060] Healthy control group (referred to as “control group”): 20 dairy goats with “-” mastitis test results were selected, fed normally, and observed.

[0061] Chinese medicine compound treatment group (referred to as "Chinese medicine group"): 20 dairy goats with mastitis test results of "+++" or "++" were selected for Chinese medicine compound treatment. 30 g of the spray-dried powder in Example 1 was dissolved in 100 ml of water to prepare a suspension, and 10 ml / goat / day was orally administered for 7 consecutive days.

[0062] Vaccine immunization control group (referred to as "vaccine group"): 20 goats with mastitis test results of "+++" or "++" were selected for streptococcal vaccine immunization, with 5 ml / goat injected intramuscularly only on the first day of the experiment.

[0063] 1.2 Evaluation criteria for sheep latent mastitis

[0064] Table 1 shows the scoring criteria for sheep subclinical mastitis.

[0065] Table 1 Criteria for determining sheep subclinical mastitis (CMT)

[0066]

[0067]

[0068] 1.3 Evaluation of the therapeutic effect of traditional Chinese medicine compound

[0069] The effectiveness of drug treatment is determined based on the CMT test results, and the treatment effects are divided into cure, effectiveness, and ineffectiveness.

[0070] After CMT testing, if the result of mastitis dairy goats changes from “+++” to “-” or from “++” to “-”, they are judged to be cured; if it changes from “+++” to “++”, “+”, “±” or from “++” to “+”, “±”, it is judged to be effective.

[0071] Cure rate % = (number of cured sheep in this group / number of treated sheep in this group) × 100%;

[0072] Effective rate % = (number of effective sheep in this group / number of treated sheep in this group) × 100%;

[0073] Total effective rate % = cure rate % + effective rate %.

[0074] 1.4 Evaluation of the therapeutic effect of traditional Chinese medicine compound

[0075] After the Chinese medicine was continuously administered for 7 days, the subclinical mastitis test (CMT method) of dairy goats was recorded on the 4th and 7th days, and the cure rate, effective rate and total effective rate within 7 days were calculated.

[0076] 1.5 Safety evaluation of traditional Chinese medicine compound

[0077] During the experiment, the body temperature, heart rate, respiratory rate, and clinical indicators related to the number of single rumination chewing of dairy goats in each group were recorded every day. Six dairy goats were randomly selected from each group on the 1st and 7th days of the experiment, and blood was collected from the jugular vein to test blood routine related indicators and milk cell count to evaluate the safety of traditional Chinese medicine. Blood routine indicators: white blood cell count (WBC), red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width variation coefficient (RDW).

[0078] 1.6 Data Analysis

[0079] The experimental data were statistically analyzed using Graphpad prism 9, using one-way analysis of variance (ANOVA), Tukey's multiple comparisons and independent T test. The data were expressed as "mean ± SD". P < 0.05 was considered to be statistically significant.

[0080] 2 Results

[0081] 2.1 The therapeutic effect of Chinese herbal compound on sheep subclinical mastitis

[0082] As shown in Table 2, on the 4th day of the test, 2 were cured in the Chinese medicine group, and 14 showed improvement. On the 7th day of the test, 9 were cured, 5 showed improvement, and 11 were cured in total, with a cure rate of 55%, an effective rate of 25%, and a total effective rate of 80%. On the 4th day of the test, 4 were cured in the vaccine group, and 11 showed improvement. On the 7th day of the test, 3 were cured, 9 showed improvement, and 7 were cured in total, with a cure rate of 35%, an effective rate of 45%, and a total effective rate of 80%. The results show that the Chinese medicine compound of the invention shows obvious therapeutic effect in the treatment of sheep subclinical mastitis.

[0083] Table 2 Effect of Chinese herbal compound on cure time and cure rate of dairy goats with invisible mastitis

[0084]

[0085] 2.2 Effect of Chinese herbal compound on the number of milk somatic cells in dairy goats with invisible mastitis

[0086] As shown in Table 3, on the first day of the experiment, the number of somatic cells in the milk of the goats in the control group was low and within the normal range; the number of somatic cells in the milk of the goats in the Chinese medicine group and the vaccine group was within the range of latent mastitis. On the seventh day of the experiment, the number of somatic cells in the milk of the goats in the control group increased to varying degrees, but was still within the normal range; the number of somatic cells in the milk of the goats in the Chinese medicine group decreased to varying degrees, of which 3 were below 500,000 / ml and 1 was above 500,000 / ml; the number of somatic cells in the milk of the goats in the vaccine group decreased to varying degrees, of which 2 were below 500,000 / ml and 2 were above 500,000 / ml.

[0087] Table 3 Changes in the number of somatic cells in the milk of 12 goats (10,000 / ml) at the beginning and end of the experiment

[0088]

[0089]

[0090] Note: * indicates that the milk somatic cell count exceeds 500,000 / ml, which is within the range of subclinical mastitis.

[0091] 2.3 Effect of Chinese herbal compound on daily milk production of dairy goats with invisible mastitis

[0092] As shown in Table 4, the daily milk production of dairy goats in the Chinese medicine group and the vaccine group was lower than that in the control group, but there was no significant difference. As the treatment time increased, the daily milk production of dairy goats in the Chinese medicine group and the vaccine group increased slightly, but there was no significant difference.

[0093] Table 4 Effect of Chinese herbal compound on daily milk production of dairy goats with invisible mastitis (kg)

[0094]

[0095] Note: Data in different groups at the same time with different lowercase letters indicate significant differences (P<0.05), while data with the same lowercase letters indicate no significant differences (P>0.05); data in the same group at different times with different uppercase letters indicate significant differences (P<0.05), while data with the same uppercase letters indicate no significant differences (P>0.05).

[0096] 2.4 Effect of Chinese herbal compound on body temperature of dairy goats with invisible mastitis

[0097] As shown in Table 5, compared with the control group, the body temperature of the dairy goats in the Chinese medicine group was significantly higher than that of the control group within 1-3 days of the experiment (P < 0.05), but there was no significant difference in body temperature between the control group and the vaccine group during the experiment (P < 0.05). As the treatment time increased, the body temperature of the dairy goats in the Chinese medicine group within 5-7 days of the experiment was significantly lower than that within 1-4 days of the experiment (P < 0.05), and there was no significant change in the body temperature of the dairy goats in the control group and the vaccine group.

[0098] Table 5 Effect of Chinese herbal compound on body temperature of dairy goats with invisible mastitis (℃)

[0099]

[0100] Note: Data in different groups at the same time with different lowercase letters indicate significant differences (P<0.05), while data with the same lowercase letters indicate no significant differences (P>0.05); data in the same group at different times with different uppercase letters indicate significant differences (P<0.05), while data with the same uppercase letters indicate no significant differences (P>0.05).

[0101] 2.5 Effect of Chinese herbal compound on heart rate of dairy goats with invisible mastitis

[0102] As shown in Table 6, there was no significant change trend in the heart rate of dairy goats among the groups, and the differences were not significant.

[0103] Table 6 Effect of Chinese herbal compound on heart rate of dairy goats with invisible mastitis (beats / min)

[0104]

[0105] Note: Data in different groups at the same time with different lowercase letters indicate significant differences (P<0.05), while data with the same lowercase letters indicate no significant differences (P>0.05); data in the same group at different times with different uppercase letters indicate significant differences (P<0.05), while data with the same uppercase letters indicate no significant differences (P>0.05).

[0106] 2.6 Effect of Chinese herbal compound on respiratory rate of dairy goats with invisible mastitis

[0107] As shown in Table 7, there was no significant change trend in the respiratory rate of dairy goats among the groups, and the differences were not significant.

[0108] Table 7 Effect of Chinese herbal compound on respiratory rate of dairy goats with invisible mastitis (times / minute)

[0109]

[0110] Note: Data in different groups at the same time with different lowercase letters indicate significant differences (P<0.05), while data with the same lowercase letters indicate no significant differences (P>0.05); data in the same group at different times with different uppercase letters indicate significant differences (P<0.05), while data with the same uppercase letters indicate no significant differences (P>0.05).

[0111] 2.7 Effect of Chinese herbal compound on the number of ruminations per time in dairy goats with invisible mastitis

[0112] As shown in Table 8, there was no significant change trend in the number of single rumination chewing of dairy goats in each group, and the difference was not significant.

[0113] Table 8 Effect of Chinese herbal compound on the number of single rumination chewing times of dairy goats with invisible mastitis (times / min)

[0114]

[0115]

[0116] Note: Data in different groups at the same time with different lowercase letters indicate significant differences (P<0.05), while data with the same lowercase letters indicate no significant differences (P>0.05); data in the same group at different times with different uppercase letters indicate significant differences (P<0.05), while data with the same uppercase letters indicate no significant differences (P>0.05).

[0117] 2.8 Effect of Chinese herbal compound on routine blood parameters of dairy goats with invisible mastitis

[0118] As shown in Table 9, on the first day of the experiment, the blood routine indicators of dairy goats in the Chinese medicine group and the vaccine group did not change significantly compared with the control group, and the difference was not obvious. On the 7th day of the experiment, the MCHC index of dairy goats in the Chinese medicine group was significantly lower than that in the control group and the vaccine group (P<0.05). On the 7th day of the experiment, compared with the 1st day of the experiment, there was no significant change in the blood indicators of dairy goats in the Chinese medicine group, the HGB and HCT indicators of dairy goats in the control group increased significantly (P<0.05), and the WBC, RBC, HGB, HCT, and RDW indicators of dairy goats in the vaccine group increased significantly (P<0.05), and they were all higher than the normal range.

[0119] Table 9 Effect of Chinese herbal compound on blood routine indexes of dairy goats with invisible mastitis

[0120]

[0121]

[0122] Note: WBC: white blood cell count; RBC: red blood cell count; HGB: hemoglobin; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin content; MCHC: mean corpuscular hemoglobin concentration; RDW: coefficient of variation of red cell distribution width; ↑ indicates that the value is beyond the normal range. Data with different lowercase letters at the same time in different groups indicate significant differences (P<0.05), and those with the same lowercase letters indicate no significant differences (P>0.05); data with different capital letters at different times in the same group indicate significant differences (P<0.05), and those with the same capital letters indicate no significant differences (P>0.05).

[0123] 3 Conclusion

[0124] The above results show that the Chinese medicine compound of the invention shows good therapeutic effect in treating sheep latent mastitis, and the Chinese medicine compound is safe and has no side effects.

[0125] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A Chinese medicine composition for preventing and treating sheep latent mastitis, characterized in that: It is made from the following ingredients: bupleurum, knotweed, dandelion, plantain, selfheal, astragalus, honeysuckle vine, and licorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The weight proportions of the components are 50-70 parts of bupleurum, 40-60 parts of knotweed, 80-99 parts of dandelion, 50-70 parts of plantain, 35-55 parts of selfheal, 20-40 parts of astragalus, 20-40 parts of honeysuckle vine and 35-55 parts of liquorice.

3. The Chinese medicine composition according to claim 1, characterized in that The weight proportions of the components are 55-65 parts of bupleurum, 45-55 parts of knotweed, 85-95 parts of dandelion, 55-65 parts of plantain, 40-50 parts of selfheal, 25-35 parts of astragalus, 25-35 parts of honeysuckle vine and 40-50 parts of liquorice.

4. The Chinese medicine composition according to claim 1, characterized in that: The weight proportions of the components are 60 parts of bupleurum, 50 parts of knotweed, 90 parts of dandelion, 60 parts of plantain, 45 parts of selfheal, 30 parts of astragalus, 30 parts of honeysuckle vine, and 45 parts of liquorice.

5. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The Chinese medicine composition is prepared by the following method: weighing each component according to the formula, crushing, countercurrent extraction, concentrating, and drying to obtain.

6. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The dosage form of the Chinese medicine composition is an oral administration dosage form.

7. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The dosage form of the Chinese medicine composition includes oral liquid, granules, powders, powders, pills, tablets or capsules.

8. The method for preparing the Chinese medicine composition according to any one of claims 1 to 4, characterized in that: According to the formula, each component is weighed, crushed, countercurrently extracted, concentrated, dried, and obtained.

9. Use of the Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a product for preventing and treating sheep latent mastitis.

10. A product for preventing and treating sheep latent mastitis, characterized in that: A Chinese medicine composition comprising any one of claims 1 to 4.

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