Novel sake lactobacillus strain and use thereof

Abstract

Novel Lactobacillus sake strains and uses thereof. The present invention relates to a Lactobacillus sake LGHNHH22021 strain, a Lactobacillus sake LGHNH2021 strain, a Lactobacillus sake LGHNH2021 strain, a Lactobacillus sake LGHNH2021 strain A cosmetic composition, a foreign medicine composition, and a food composition for improving skin, which comprise at least one of the above-mentioned bacterial strain, a culture of the above-mentioned bacterial strain, a fermented product of the above-mentioned bacterial strain, a culture solution of the above-mentioned bacterial strain, an extract of the above-mentioned bacterial strain, and a crushed product of the above-mentioned bacterial strain; a composition for promoting the proliferation of Staphylococcus epidermidis, which is a beneficial bacterium for the skin, comprising the above strain; a composition for inhibiting the proliferation of Staphylococcus aureus, which is a bacterium harmful to the skin, comprising the above strain; and a method for producing a fermented product or a dissolved product, which comprises a step of fermenting or pulverizing the strain.

Description

Technical Field

[0001] The present invention relates to Lactobacillus sakei LGHNH22021 strain; a cosmetic composition, a quasi-pharmaceutical composition and a food composition for improving skin, comprising any one or more of the strain, a culture of the strain, a fermented product of the strain, a culture solution of the strain, an extract of the strain and a crushed product of the strain; a composition comprising the strain for promoting the proliferation of Staphylococcus epidermidis, which is a beneficial bacteria for the skin; a composition comprising the strain for inhibiting the proliferation of Staphylococcus aureus, which is a harmful bacteria for the skin; and a method for preparing a fermented product or a dissolved product comprising the steps of fermenting or crushing the strain. Background Art

[0002] There are many symbiotic microorganisms in living organisms. Among these symbiotic microorganisms, some produce lactic acid as a metabolite while growing, and these bacteria are collectively referred to as lactic acid bacteria. They are widely distributed in various organs such as the digestive tract, oral cavity, vagina of humans and mammals, as well as in nature such as plants, various fermented foods and soil. These lactic acid bacteria are microorganisms that have been used by humans for a long time and are used in various fermented foods (fermented dairy products, fermented foods, sauces, kimchi, etc.). Recently, the attention paid to the efficacy of lactic acid bacteria has been increasing, and research on the use of lactic acid bacteria not only in food materials but also in cosmetics and pharmaceuticals is being actively carried out.

[0003] Materials mainly made of terrestrial plants used in cosmetics and quasi-pharmaceuticals require a lot of money and time to cultivate, but microorganisms are highly sustainable materials that can be cultivated forever after being obtained once. In addition, even biological resources of the same genus have different characteristics and effects depending on the strain and origin, so it is necessary to explore as many biological resources as possible and use them according to their characteristics.

[0004] In this regard, research is being conducted to use plant lactobacillus strains (KR 10-2022-0081475A) and fermentative lactobacillus strains (KR 10-2022-0170253A) belonging to the genus Lactobacillus as cosmetic raw materials, but the industrial fields in which lactic acid bacteria are applied are still extremely limited in terms of their scale and diversity compared to the rich species, so there is a need to expand the application fields.

[0005] Under such circumstances, the present inventors have studied lactic acid bacteria from natural resources that are safer and have excellent skin-improving effects, and have confirmed that the novel sake Lactobacillus LGHNH22021 strain has a significantly excellent skin-improving effect, thereby completing the present invention. Summary of the invention

[0006] Issues to be resolved

[0007] An object of the present invention is to provide a Lactobacillus sakei LGHNH22021 strain.

[0008] Another object of the present invention is to provide a cosmetic composition for improving the skin comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain.

[0009] Another object of the present invention is to provide a quasi-pharmaceutical composition for improving skin comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain.

[0010] Another object of the present invention is to provide a food composition for improving skin comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain.

[0011] Another object of the present invention is to provide a composition for promoting the proliferation of Staphylococcus epidermidis, which is a beneficial bacteria for the skin, comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain.

[0012] Another object of the present invention is to provide a composition for inhibiting the proliferation of Staphylococcus aureus, which is a skin-harmful bacterium, comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain, and the crushed product of the above-mentioned strain.

[0013] Another object of the present invention is to provide a method for preparing a fermented product comprising the step of fermenting the above strain.

[0014] Another object of the present invention is to provide a method for preparing a lysate comprising the step of crushing the above-mentioned strain.

[0015] Another object of the present invention is to provide a method for improving skin condition, comprising the step of applying a composition comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain to the skin of an individual.

[0016] Another object of the present invention is to provide a composition for improving skin condition comprising any one or more of the above-mentioned strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain.

[0017] Another object of the present invention is to provide a skin improvement use of Lactobacillus sakei LGHNH22021 strain preserved under the deposit number KCTC 15087BP.

[0018] Solution to the problem

[0019] It is described in detail as follows. On the other hand, each description and embodiment disclosed in the present invention can also be applied to each different description and embodiment. That is, all combinations of the various elements disclosed in the present invention belong to the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific description described below.

[0020] Additionally, those skilled in the art may be able to identify or ascertain, using no more than routine experimentation, multiple equivalents to the specific aspects of the invention described herein. Additionally, such equivalents are intended to be encompassed by the present invention.

[0021] One aspect of the present invention provides a Lactobacillus sakei LGHNH22021 strain deposited under the accession number KCTC 15087BP.

[0022] As a specific example, the above-mentioned strain may have one or more characteristics selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0023] In the present invention, the term "Lactobacillus sakei" refers to an aerobic or facultative anaerobic Gram-positive microorganism of the genus Bacillus that is widely distributed in nature.

[0024] The present inventors developed a novel strain belonging to Lactobacillus sakei, named it Lactobacillus sakei LGHNH22021, and deposited it under the accession number KCTC 15087BP.

[0025] Specifically, the novel sake Lactobacillus LGHNH22021 strain of the present invention is the core-microbiome of Achilleamillefolium and can be obtained by isolation from Achilleamillefolium, but is not limited thereto, as long as it has the same characteristics as the preserved strain sake Lactobacillus LGHNH22021.

[0026] In the present invention, the method for isolating the above strain from Achillea millefolium is not particularly limited, and the strain having the same characteristics as the above deposited strain can be isolated according to the commonly used methods in the technical field or similar fields.

[0027] The term "strain" of the present invention refers to a collection of individuals derived from one cell, proliferated by asexual reproduction and having homogeneous genetic characteristics, and there are multiple strains (genetic variants) with different genetic characteristics within one species. In the present invention, the strain refers to Lactobacillus sakei LGHNH22021, specifically Lactobacillus sakei LGHNH22021 deposited with the accession number KCTC 15087BP, but is not limited thereto.

[0028] In the present invention, the strain may include the cell, dried cell, etc. of the Lactobacillus sakei LGHNH22021 strain. In this case, the dried cell may be spray-dried, freeze-dried, vacuum-dried, drum-dried, or other dried cells.

[0029] Another aspect of the present invention provides a cosmetic composition for improving skin comprising any one or more of the sake Lactobacillus LGHNH22021 strain, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a crushed product of the strain.

[0030] Specifically, the present inventors confirmed that the Lactobacillus sakei LGHNH22021 strain derived from yarrow ( Achillea millefolium ) has an excellent skin-improving effect.

[0031] The term "culture" of the present invention refers to a product obtained by culturing the above-mentioned strain in a culture medium. As an example, the culture of the present invention may include components remaining in the culture medium remaining after harvesting the above-mentioned strain from the culture medium of Lactobacillus sakei LGHNH22021, or components of the culture medium containing the strain.

[0032] The term "culture fluid" of the present invention refers to the part of the above-mentioned culture except the strain. As an example, the culture fluid of the present invention may include the culture supernatant except the above-mentioned strain after centrifugation in the culture medium of Lactobacillus sakei LGHNH22021, or the part of the culture supernatant except the crushed product.

[0033] The culture may be the sake Lactobacillus LGHNH22021 strain, the whole culture of the strain, a culture medium, a fraction thereof, etc. The culture solution may be the culture supernatant of the sake Lactobacillus LGHNH22021 strain, etc. At this time, the culture supernatant may be obtained by centrifuging the culture of the strain, and the fraction may be obtained by applying centrifugation, chromatography, etc. to the strain, the culture, the culture supernatant, etc.

[0034] The culture medium and other culture conditions used to culture the strain of the present invention can be any without particular limitation, as long as it is a conventional culture medium for culturing Lactobacillus microorganisms. Specifically, the strain of the present invention can be cultured under aerobic or anaerobic conditions in a conventional culture medium containing an appropriate carbon source, nitrogen source, phosphorus source, inorganic compound, amino acid and / or vitamin, etc., while adjusting the temperature, pH, etc.

[0035] In the present invention, as the above-mentioned carbon source, carbohydrates such as glucose, fructose, sucrose, maltose, etc.; sugar alcohols such as mannitol, sorbitol, etc.; organic acids such as pyruvic acid, lactic acid, citric acid, etc.; amino acids such as glutamic acid, methionine, lysine, etc., but not limited thereto. In addition, natural organic nutrient sources such as starch hydrolysates, molasses, blackstrap molasses, rice bran, cassava, bagasse, and corn steep liquor can be used, carbohydrates such as glucose and sterilized pretreated molasses (i.e., molasses converted into reducing sugars), etc. can be used, and other appropriate carbon sources can be used in a variety of ways without limitation. These carbon sources can be used alone or in combination of two or more.

[0036] As the nitrogen source, inorganic nitrogen sources such as ammonia, ammonium sulfate, ammonium chloride, ammonium acetate, ammonium phosphate, ammonium carbonate, ammonium nitrate, etc.; organic nitrogen sources such as amino acids, peptone, NZ-amine, meat extract, yeast extract, malt extract, corn steep liquor, casein hydrolyzate, fish or its decomposition product, defatted soybean cake or its decomposition product, etc. can be used. These nitrogen sources can be used alone or in combination of two or more, but are not limited thereto.

[0037] As the phosphorus source, potassium dihydrogen phosphate, dipotassium hydrogen phosphate or their corresponding sodium salts may be included, etc. As the inorganic compound, sodium chloride, calcium chloride, ferric chloride, magnesium sulfate, ferric sulfate, manganese sulfate, calcium carbonate, etc. may be used.

[0038] In addition, the above-mentioned culture medium may contain amino acids, vitamins and / or appropriate precursors, etc. Specifically, L-amino acids, etc. may be added to the culture medium of the above-mentioned strain. Specifically, glycine, glutamate and / or cysteine, etc. may be added, and L-amino acids such as lysine may also be added if necessary, but are not necessarily limited thereto.

[0039] The above-mentioned culture medium or precursor may be added to the culture or culture solution in a batch manner or a continuous manner, but is not limited thereto.

[0040] In the present invention, in the cultivation of bacterial strains, the pH of the culture or culture solution can be regulated by adding compounds such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid, sulfuric acid in a suitable manner to the culture or culture solution. In addition, in the cultivation, the generation of foam can be suppressed by using defoamers such as fatty acid polyethylene glycol esters. In addition, in order to maintain the aerobic state of the culture or culture solution, oxygen or oxygen-containing gas can be injected into the culture or culture solution, or in order to maintain anaerobic and microaerobic state, nitrogen, hydrogen or carbon dioxide gas can be injected without injecting gas.

[0041] The term "fermented product" of the present invention may include a culture medium containing a strain, its metabolites, additional nutrients, etc. obtained by culturing a microorganism in a culture medium for a certain period of time, a culture solution from which the strain is removed, a dilution or concentrate of the culture medium or culture solution, or a dried product thereof. The above-mentioned "fermentation" refers to those processes that are not decay reactions in which organic matter is decomposed using enzymes possessed by the microorganism itself.

[0042] In the present invention, the method for obtaining the fermentation product from the above-mentioned strain is not particularly limited, and can be obtained according to a method commonly used in the present technical field or similar fields.

[0043] In the present invention, the method for obtaining the above-mentioned fermentation product can be obtained by treating the above-mentioned isolated strain on the energy source of the strain, i.e., sugar, and fermenting it, but the temperature, fermentation time, etc. of the above-mentioned fermentation process are not particularly limited, and can be selected in various ways depending on the fermentation precursor substances, fermentation conditions, the types of substances to be obtained from the fermentation product, etc.

[0044] In the present invention, the fermentation process can be carried out at a temperature of 20°C to 45°C, more specifically 25°C to 35°C, and further specifically 27°C to 33°C. The fermentation period can be 1 to 14 days, more specifically 1 to 3 days, and further specifically 1 to 2 days.

[0045] In the present invention, the fermentation product obtained from the above-mentioned strain includes not only the fermented substance itself, but also all kinds of substances including the fermentation product produced by the above-mentioned strain, for example, the culture medium of the strain in which the strain and the culture coexist, the fermentation product of the strain filtered from the above-mentioned culture medium, the supernatant except the strain after centrifuging the above-mentioned culture medium, the fermentation product obtained by sterilizing the strain from the above-mentioned culture medium and filtering it, the extract of the above-mentioned fermentation product or the culture medium containing it, the dilution of the above-mentioned fermentation product or its extract, the dried product of the above-mentioned fermentation product or its extract, the lysate of the bacterial body of the above-mentioned strain collected and crushed, etc.

[0046] In the present invention, for the concentration of the fermentation product of Lactobacillus sake LGHNH22021, the weight % of the above-mentioned Lactobacillus sake LGHNH22021 strain can be 0.000001% to 100%, 0.000001% to 50%, 0.000001% to 40%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15%, 0.000001% to 10%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15 ...30%, 0.000001% to 30%, 0.000001% to 30%, 0.000001% to 30%, 0.000001% to 30%, 0.000001% to 30%, 0.01% to 10%, 0.0001% to 30%, 0.0001% to 20%, 0.0001% to 15%, 0.0001% to 10%, 0.001% to 30%, 0.001% to 20%, 0.001% to 15%, 0.001% to 10%, 0.01% to 30%, 0.01% to 20%, 0.01% to 15%, 0.01% to 10%, 0.1% to 30%, 0.1% to 20%, 0.1% to 15%, 0.1% to 10%, 1% to 30%, 1% to 20%, 1% to 15%, or 1% to 10%, but is not limited to these.

[0047] The term "extract" in the present invention refers to a product such as a liquid component obtained by immersing a target substance in various solvents and then extracting it for a certain period of time at room temperature, low temperature or heating, a solid component obtained by removing the solvent from the above liquid component, etc. In addition, in addition to the above products, it can be generally interpreted as including all dilutions, concentrated solutions, crude and refined products, refined products, etc. of the above products. Therefore, the sake Lactobacillus LGHNH22021 extract provided in the present invention can be interpreted as including an extract obtained by performing an extraction treatment on it, a dilution or concentrated solution of the above extract, a dry product obtained by drying the above extract, a crude or refined product of the above extract, or a mixture thereof, etc., the extract itself and extracts in all dosage forms that can be formed using the extract.

[0048] The method for extracting sake Lactobacillus LGHNH22021 of the present invention is not particularly limited, and can be extracted according to a method commonly used in the art. As non-limiting examples of the above-mentioned extraction method, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc. can be cited, which can be performed alone or in combination of two or more methods.

[0049] The type of solvent used for the above-mentioned extraction is not particularly limited, and any solvent known in the art can be used. As non-limiting examples of the above-mentioned extraction solvent, water, C1 to C4 lower alcohol, ether, ethyl acetate, glycerine, butylene glycol, propylene glycol, methyl acetate, ethyl acetate, chloroform, acetone, benzene, hexane, diethyl ether and methylene chloride or its mixed solvent etc. can be cited, these can be used alone or two or more are mixed and used, and water can be used specifically. When using alcohol as solvent, specifically alcohol with a carbon number of 1 to 4 can be used.

[0050] In the present invention, the extract may be an extract of the above-mentioned culture, fermentation product, culture broth, crushed product or strain (precipitate, pellet), specifically, an extract extracted by vacuum concentration of the strain after centrifuging the culture and removing the supernatant, but is not limited thereto.

[0051] In the present invention, for the concentration of the sake Lactobacillus LGHNH22021 extract, based on the total weight % of the composition, the weight % of the above-mentioned sake Lactobacillus LGHNH22021 strain may be 0.000001% to 100%, 0.000001% to 50%, 0.000001% to 40%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15%, 0.000001% to 10%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15%. 0.01% to 10%, 0.0001% to 30%, 0.0001% to 20%, 0.0001% to 15%, 0.0001% to 10%, 0.001% to 30%, 0.001% to 20%, 0.001% to 15%, 0.001% to 10%, 0.01% to 30%, 0.01% to 20%, 0.01% to 15%, 0.01% to 10%, 0.1% to 30%, 0.1% to 20%, 0.1% to 15%, 0.1% to 10%, 1% to 30%, 1% to 20%, 1% to 15%, or 1% to 10%, but is not limited to these.

[0052] The term "crushed material (or lysate)" of the present invention can be obtained by subjecting the strain itself to physical or chemical treatment, autoclaving or ultrasonic treatment, or can be obtained by crushing the supernatant, fermentation product or culture fluid obtained by centrifuging the culture of the strain, but is not limited thereto. The above crushed material can be used in combination with the term "lysate".

[0053] The composition of the present invention comprising the sake Lactobacillus LGHNH22021 strain preserved with KCTC 15087BP, a culture, fermentation product, culture solution, extract, crushed product or a combination thereof has an excellent skin improvement effect compared to a composition comprising other sake Lactobacillus LGHNH22021 strains, their cultures, fermentation products, culture solutions, extracts or crushed products.

[0054] In the present invention, "improving skin" refers to all actions that at least reduce the degree of parameters such as symptoms related to the alleviation or treatment of skin conditions. For example, the above-mentioned skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0055] The term "improvement" in the present invention refers to all actions that at least reduce the parameters associated with the alleviation or treatment of a condition, such as the extent of symptoms.

[0056] The above-mentioned skin improvement can be achieved by one or more selected from the group consisting of promoting collagen synthesis, promoting keratinocyte growth, inhibiting melanin synthesis, increasing hyaluronic acid production, inhibiting NO production, increasing beneficial bacteria growth and inhibiting harmful bacteria growth, but is not limited thereto.

[0057] The term "aging" of the present invention refers to the phenomenon that the structure and function of an organism decline over time, and for the "anti-aging" of the present invention, if aging is improved, such as prevention, inhibition, delay of aging, etc., it is not limited to its degree, etc. The above-mentioned anti-aging may include improving skin elasticity, regenerating skin, improving wrinkles, etc., but is not limited thereto.

[0058] The term "skin wrinkles" in the present invention refers to the phenomenon of reduced skin elasticity and skin wrinkles due to skin aging, and "improving wrinkles" in the present invention may refer to inhibiting or preventing the formation of wrinkles on the skin, or alleviating wrinkles that have already occurred, but is not limited thereto.

[0059] The term "skin elasticity" of the present invention is the force that keeps the skin taut when pressing with fingers, and "improving elasticity" or "enhancing elasticity" means improving the elasticity of the skin by strengthening the subcutaneous fat layer structure of an individual, and may refer to improving the elasticity of the skin using the composition of the present invention. Specifically, anti-aging and improving elasticity may be due to the promotion of collagen synthesis, but is not limited thereto.

[0060] The term "collagen" in the present invention is a fibrous protein that is common in most animals, especially mammals, and occupies most of all connective tissues in the body, such as skin and cartilage. The most common cells in the body, fibroblasts, produce and secrete collagen. Gelatin, which is widely used in cooking or the food and pharmaceutical industries, is irreversibly hydrolyzed from collagen. The reduction of collagen is the main cause of wrinkles and reduced skin elasticity, so the synthesis of collagen is crucial for improving wrinkles and enhancing elasticity.

[0061] In the present invention, the promotion of collagen synthesis may be due to the sake Lactobacillus LGHNH22021 strain, the culture of the above strain, the fermentation product of the above strain, the culture liquid of the above strain, the extract of the above strain, the crushed material of the above strain or a mixture thereof, and the composition of the present invention can have an anti-aging effect by promoting the above collagen synthesis.

[0062] In one embodiment of the present invention, it was confirmed that the lysate and fermentation product of the novel sake Lactobacillus LGHNH22021 strain of the present invention had excellent collagen synthesis promoting effect compared with the conventionally known sake Lactobacillus comparison strain KCTC3603, thereby confirming that the sake Lactobacillus LGHNH22021 strain had significant anti-aging effects.

[0063] In the present invention, the term "regenerated skin" refers to the process of skin tissue recovering from damage caused by external and internal causes of the skin. The damage caused by the external causes may be exemplified by ultraviolet rays, external pollutants, trauma, trauma, etc., and the damage caused by the internal causes may be exemplified by stress, etc. Specifically, regenerated skin may be due to the promotion of keratinocyte growth, but is not limited thereto.

[0064] In the present invention, the promotion of keratinocyte growth may be due to the sake Lactobacillus sakei LGHNH22021 strain, the culture of the above strain, the fermentation of the above strain, the culture solution of the above strain, the extract of the above strain, the crushed material of the above strain or a mixture thereof, and the composition of the present invention can have a skin regeneration effect by promoting the growth of the above keratinocytes.

[0065] In one embodiment of the present invention, it was confirmed that the lysate and fermentation product of the novel sake Lactobacillus LGHNH22021 strain of the present invention had excellent keratinocyte growth promoting effect compared with the conventionally known sake Lactobacillus comparison strain KCTC3603, thereby confirming that the sake Lactobacillus LGHNH22021 strain had a significant skin regeneration effect.

[0066] The term "whitening" in the present invention means a method of increasing the brightness of skin whose brightness is reduced due to excessive pigments such as melanin or maintaining the brightness of the skin at a certain level, skin with increased brightness formed by the above method, etc., and specifically refers to skin whitening.

[0067] In the present invention, the term "skin whitening" means not only brightening the skin color but also improving skin hyperpigmentation such as dark spots or freckles caused by ultraviolet rays, hormones or genetics by inhibiting the synthesis of melanin pigment.

[0068] The term "melanin" in the present invention is one of the common pigments in nature, which is dark brown or black. The amount of melanin determines the skin color. The more melanin, the darker the skin color. It is synthesized from tyrosine, one of the amino acids, to form a polyphenol polymer, forming a very stable structure. Due to such structural characteristics, in addition to resistance to heat and chemicals, antioxidant capacity, it also has resistance to radiation such as ultraviolet rays.

[0069] In the present invention, the inhibition of melanin synthesis may be due to the sake Lactobacillus LGHNH22021 strain, the culture of the above strain, the fermentation product of the above strain, the culture liquid of the above strain, the extract of the above strain, the crushed material of the above strain or a mixture thereof, and the composition of the present invention can have a whitening effect by inhibiting the above-mentioned melanin synthesis.

[0070] In one embodiment of the present invention, it was confirmed that the lysate or fermentation product of the novel sake Lactobacillus LGHNH22021 strain of the present invention had an excellent melanin synthesis inhibitory effect compared with the conventionally known sake Lactobacillus comparison strain KCTC3603, thereby confirming that the sake Lactobacillus LGHNH22021 strain had a significant skin whitening effect.

[0071] In the present invention, the term "moisturizing skin" refers to all actions to maintain the softness of the skin by supplying moisture to the skin or blocking the evaporation of moisture and to maintain a smooth surface by inducing uniform keratin exfoliation, and "dryness" refers to a state in which the skin is not sufficiently moisturized. The skin moisturizing effect can help improve wrinkles and increase elasticity of the skin. Specifically, skin moisturizing may be due to an increase in hyaluronic acid production and / or an increase in the expression of aquaporin 3 (AQP3), but is not limited thereto.

[0072] In the present invention, the term "hyaluronic acid" is a glycosaminoglycan, which refers to a chain-like high-molecular polysaccharide substance formed by repeated connection of glucuronic acid and N-acetylglucosamine residues. Specifically, hyaluronic acid is synthesized by hyaluronan synthase HAS2 (hyaluronan synthase 2). Therefore, when HAS2 expression is increased, the synthesis of hyaluronic acid can be promoted.

[0073] In the present invention, the term "aquaporin 3 (AQP3)" is an integral membrane protein that forms a channel in the cell membrane and induces passive transport of water molecules, selectively passing only water molecules while restricting the movement of other substances.

[0074] In the present invention, the increase in hyaluronic acid production and / or the increase in aquaporin 3 expression may be due to the sake Lactobacillus sakei LGHNH22021 strain, the culture of the above strain, the fermentation product of the above strain, the culture liquid of the above strain, the extract of the above strain, the crushed product of the above strain or a mixture thereof, and the composition of the present invention can have a skin moisturizing effect through the increase in hyaluronic acid production and / or the increase in aquaporin 3 expression.

[0075] In one embodiment of the present invention, it was confirmed that the solubilized product or fermented product of the novel sake Lactobacillus LGHNH22021 strain of the present invention had excellent hyaluronic acid production increase and / or water channel protein 3 expression increase effect compared with the conventionally known sake Lactobacillus comparison strain KCTC3603, thereby confirming that the sake Lactobacillus LGHNH22021 strain had a significant skin moisturizing effect.

[0076] The term "skin calming" in the present invention refers to relieving and stabilizing erythema or irritated skin parts, etc., and may include the concept of relieving skin irritation. For example, it may include reducing skin moisture loss and / or reducing skin redness, but is not limited thereto.

[0077] The term "inflammation" in the present invention is an in vivo response to damage or infection of a specific tissue, and the main mediator is immune cells. The purpose of this inflammation is to minimize tissue damage, remove infected bodies, and regenerate tissues. Specifically, calming the skin and anti-inflammation may be due to the inhibition of NO production, but it is not limited thereto.

[0078] The term "NO (nitric oxide, nitrous acid)" of the present invention plays a variety of roles such as removing bacteria and tumors, regulating blood pressure or mediating neurotransmission, but if an inflammatory reaction occurs, the expression of iNOS (inducible nitric oxide synthase) in the relevant cells increases, thereby generating a large amount of NO, and the excessively generated NO induces tissue damage, gene mutation, nerve damage, etc., increases vascular permeability, and promotes inflammatory reactions such as edema. The generation of NO increases in cells that induce inflammation, and if the skin is calmed, the generation of NO is suppressed, so they have a necessary correlation.

[0079] In the present invention, the inhibition of NO production may be due to the sake Lactobacillus LGHNH22021 strain, the culture of the above strain, the fermentation product of the above strain, the culture solution of the above strain, the extract of the above strain, the crushed product of the above strain or a mixture thereof, and the composition of the present invention can have skin calming and inflammation improvement effects through the above-mentioned inhibition of NO production.

[0080] In one embodiment of the present invention, it was confirmed that the solubilized product or fermented product of the novel sake Lactobacillus LGHNH22021 strain of the present invention has an excellent effect of inhibiting the production of nitric oxide (NO) (which is a representative redness-inducing substance and induces skin irritation as an inflammatory substance) compared with the conventionally known sake Lactobacillus comparison strain KCTC3603, thereby confirming that the sake Lactobacillus LGHNH22021 strain has significant skin calming and inflammation improvement effects.

[0081] The term "microbiome" of the present invention refers to all genetic information of microorganisms inhabiting the human body or the microorganisms themselves, and is a composite word of microbiota and genome, which are symbiotic microorganisms inhabiting the human body.

[0082] The term "improving the microbiome" of the present invention can be used interchangeably as maintaining the balance of the microbiome, which means maintaining the balance of the above-mentioned microbiome, and the balance of the microbiome can be maintained by promoting the growth of beneficial bacteria while inhibiting the growth of harmful bacteria. If the balance of the microbiome is destroyed, the skin barrier will be weakened, thereby facilitating the proliferation of harmful microorganisms. Specifically, improving the microbiome may be due to an increase in the growth of beneficial bacteria and / or an inhibition of the growth of harmful bacteria, but is not limited thereto.

[0083] In the present invention, the term "beneficial bacteria for skin" refers to a bacterial strain that helps maintain skin homeostasis (homeostasis) by enhancing skin defense by enhancing skin regeneration effect, and by destroying (death) and inhibiting the growth of harmful bacteria. For example, the beneficial bacteria may be Staphylococcus epidermidis, Staphylococcus warneri, Streptococcus mitis, Micrococcus luteus, Acinetobacter johnsonii, etc. Specifically, it may be Staphylococcus epidermidis, but is not limited thereto. More specifically, the beneficial bacteria may be Staphylococcus epidermidis, but is not limited thereto.

[0084] The above-mentioned Staphylococcus epidermidis enhances the skin's defense by inducing the expression of antimicrobial peptides (AMP) from skin epidermal cells and enhancing the skin's regeneration effect; when treating the component PSM (phenol soluble modulin) secreted by Staphylococcus epidermidis, skin regeneration is enhanced by destroying (killing) the cell membrane of harmful bacteria and inhibiting their growth; butyric acid, a component secreted by Staphylococcus epidermidis, inhibits the expression of inflammatory factors induced by ultraviolet rays, thereby showing a skin calming effect; when live bacteria are applied to the skin, the effect of enhancing the moisture content of the skin and inhibiting moisture loss is shown; when live bacteria are applied to the skin, the pH of the skin can be weakly acidified, thereby improving the pH environment, so the substance having the enhancing effect of the above-mentioned Staphylococcus epidermidis as a beneficial bacteria has a beneficial effect on the skin.

[0085] In the present invention, the term "promoting the proliferation of beneficial bacteria on the skin" refers to promoting or improving the growth of the aforementioned beneficial bacteria. For the purpose of the present invention, the proliferation promotion of the aforementioned beneficial bacteria may be the proliferation promotion of Staphylococcus epidermidis, but is not limited thereto.

[0086] The term "skin harmful bacteria" in the present invention refers to bacteria that cause skin inflammation, acne, etc. The harmful bacteria may be, for example, Staphylococcus aureus, but is not limited thereto.

[0087] In the present invention, the term "inhibiting the proliferation of harmful bacteria on the skin" means inhibiting the growth of the aforementioned harmful bacteria. For the purpose of the present invention, the inhibition of the proliferation of the aforementioned harmful bacteria may be inhibition of the proliferation of Staphylococcus aureus, but is not limited thereto.

[0088] In the present invention, the promotion of the proliferation of beneficial bacteria on the skin and / or the inhibition of the proliferation of harmful bacteria on the skin may be due to the sake Lactobacillus LGHNH22021 strain, the culture of the above strain, the fermentation product of the above strain, the culture solution of the above strain, the extract of the above strain, the crushed material of the above strain or a mixture thereof, and the composition of the present invention can have a microbiome improvement effect by promoting the proliferation of the above-mentioned beneficial bacteria on the skin and / or inhibiting the proliferation of harmful bacteria on the skin.

[0089] In one embodiment of the present invention, it was confirmed that the sake Lactobacillus LGHNH22021 strain promoted the proliferation of Staphylococcus epidermidis, which is a beneficial bacterium, and inhibited the growth of Staphylococcus aureus, which is a harmful bacterium, thereby confirming that the sake Lactobacillus LGHNH22021 strain has a microbiome improvement effect.

[0090] The term "cosmetic composition" of the present invention can be a dosage form selected from the group consisting of solution, external agent, cream, foam, nourishing lotion, skin softener, perfume, facial mask, softening water, emulsion, primer, essence, soap, liquid cleanser, bath preparation, sunscreen, sunscreen oil, suspension, emulsion, paste, gel, dew, powder, soap, cleanser containing surfactant, oil, powder foundation, emulsion foundation, wax foundation, patch and spray, but is not limited thereto.

[0091] In the present invention, the term "external agent" refers to a preparation provided for external use, including skin external agent, external powder, external tablet, external liquid, external ointment, external plaster, external suppository, etc. External agent refers to a preparation such as zinc white (sub-lead) starch that is spread on erosions, ulcers, etc. of the skin and mucous membranes, external tablets refer to dissolving tablets or vaginal tablets, and external liquid refers to a liquid preparation with water, ethanol, oils, etc. as solvents, which is used for brushing teeth, wet compresses, cleaning, eye drops, nasal drops, etc. In addition, an ointment is a semisolid agent of appropriate consistency applied to the skin, a plaster is a skin-applicable agent that is solid at room temperature and softened at body temperature, and a suppository is a preparation suitable for the anus, vagina, and urethra.

[0092] The above-mentioned "skin external preparation" refers to a preparation that acts on the skin among external preparations, and in the present invention, refers to a skin external preparation containing the above-mentioned isoascorbic acid compound or an acceptable salt thereof as an effective ingredient. Specifically, among the skin external preparations, a pharmaceutical or quasi-pharmaceutical product in the form of a skin external preparation in the form of a cream, gel, patch, spray, ointment, plaster, lotion, liniment, paste or papule can be used, but it is not limited thereto.

[0093] The cosmetic composition of the present invention may further contain one or more cosmetically acceptable carriers generally used in skin cosmetics. As conventional ingredients, for example, oil, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately added, but are not limited to these.

[0094] The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the dosage form of the cosmetic composition.

[0095] When the cosmetic dosage form of the present invention is an ointment, cream, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silicon dioxide, talc, zinc oxide, etc. can be used, but not limited to these. These can be used alone or in combination of two or more.

[0096] When the dosage form of the present invention is a solution or an emulsion, as a carrier component, a solvent, a solubilizing agent or an emulsifier can be used, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, etc. can be used, especially cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan can be used, but it is not limited thereto. These can be used alone or in combination of two or more.

[0097] When the dosage form of the present invention is a suspension, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth gum can be used, but are not limited thereto. These can be used alone or in combination of two or more.

[0098] When the dosage form of the present invention is soap, as the carrier component, alkali metal salts of fatty acids, fatty acid half ester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. can be used, but are not limited thereto. These can be used alone or in combination of two or more.

[0099] When the dosage form of the present invention is powder or spray, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof can be used as a carrier component. In particular, when it is a spray, a propellant such as chlorofluorocarbon, propane / butane or dimethyl ether can be additionally contained.

[0100] On the other hand, all the ingredients described in the present invention can preferably be included in the composition of the present invention within the range not exceeding the maximum usage values ​​specified in relevant regulations such as cosmetic safety standards and China's "Cosmetic Safety·Technical Specifications".

[0101] In the cosmetic composition of the present invention, the content of the Lactobacillus sakei LGHNH22021 strain, the culture of the strain, the fermentation product of the strain, the culture solution of the strain, the extract of the strain, the crushed product of the strain or a combination thereof may be 0.000001% to 100%, 0.000001% to 90%, 0.000001% to 80%, 0.000001% to 70%, 0.000001% to 60%, 0.000001% to 50%, 0.000001% to 40%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15% based on the total weight of the cosmetic composition. , 0.000001% to 10%, 0.00001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 40%, 0.00001% to 30%, 0.00001% to 20%, 0.00001% to 15%, 0.00001% to 10%, 0.0001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 4 0%, 0.0001% to 30%, 0.0001% to 20%, 0.0001% to 15%, 0.0001% to 10%, 0.001% to 90%, 0.001% to 80%, 0.001% to 70%, 0.001% to 60%, 0.001% to 50%, 0.001% to 40%, 0.001% to 30%, 0.001% to 20%, 0.001% to 15%, 0.001% to 10%, 0.01% to 90%, 0.01% to 80%, 0.01% to 70%, 0.01% to 60%, 0.01% to 50%, 0.0 1% to 40%, 0.01% to 30%, 0.01% to 20%, 0.01% to 15%, 0.01% to 10%, 0.1% to 90%, 0.1% to 80%, 0.1% to 70%, 0.1% to 60%, 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, 0.1% to 15%, 0.1% to 10%, 1% to 90%, 1% to 80%, 1% to 70%, 1% to 60%, 1% to 50%, 1% to 40%, 1% to 30%, 1% to 20%, 1% to 15%, or 1% to 10%, but not limited thereto. For example, the content can be 0.001 to 100%, specifically 0.01 to 30%, but not limited thereto.

[0102] Another aspect of the present invention provides a quasi-pharmaceutical composition for improving skin comprising any one or more of the above-mentioned Lactobacillus sakei LGHNH22021 strain, a culture of the above-mentioned strain, a fermentation product of the above-mentioned strain, a culture solution of the above-mentioned strain, an extract of the above-mentioned strain, and a crushed product of the above-mentioned strain.

[0103] The above-mentioned “Lactobacillus sakei LGHNH22021”, “strain”, “culture”, “culture solution”, “crushed product”, “fermented product”, “extract”, “skin improvement” and the like are as described above.

[0104] Specifically, the above-mentioned quasi-pharmaceutical composition is used to improve the skin, and the above-mentioned skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0105] In the present invention, the term "quasi-pharmaceuticals" can be selected from the group consisting of shower gel, disinfecting cleaner, detergent, kitchen cleaner, cleaning cleaner, toothpaste, mouthwash, wet wipes, lotion, soap, hand soap, hair cleanser, hair softener, humidifier filler, facial mask, ointment and filter filler, but is not limited thereto.

[0106] The quasi-medical composition of the present invention may further contain a pharmaceutically acceptable carrier, excipient or diluent in addition to the above-mentioned ingredients as required. The above-mentioned pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effect of the present invention, and may include, for example, a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, a lubricant, a sweetener, a fragrance, a preservative, etc.

[0107] Representative examples of pharmaceutically acceptable carriers, excipients or diluents of the present invention can include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerol, gum arabic, alginate, calcium phosphate, calcium carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil, injectable esters, witepsol, macrogol, Tween 61, cocoa butter, laurin butter, etc.

[0108] When the bacterial strain of the present invention or its fermented product is used as a medicine, it can further contain more than one active ingredient showing the same or similar function. For example, it can include known ingredients for enhancing beneficial skin bacteria, inhibiting harmful skin bacteria, calming skin, improving skin inflammation, whitening skin, regenerating skin, and healing wounds. If additional wrinkle improvement, skin whitening, skin problem improvement, and moisturizing skin ingredients are included, the antioxidant, whitening, anti-aging, skin elasticity improvement, microbiome improvement, moisturizing skin, calming skin, inflammation improvement, and skin barrier strengthening effects of the composition of the present invention can be further increased. When adding the above-mentioned ingredients, it can be considered that the skin safety caused by composite use, the ease of formulation, and the stability of the active ingredient.

[0109] The pharmaceutical composition of the present invention may further include retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extracts selected from the group consisting of known skin aging ingredients in the art; non-steroidal anti-inflammatory ingredients such as flufenamic acid, ibuprofen, benzydamine, indomethacin, prednisolone, dexamethasone, allantoin, azulene, hydrocortisone; and their derivatives and various plant extracts. Based on the weight of the total composition, the content of the additional ingredients can be 0.0001% by weight to 10% by weight, and the above content range can be adjusted according to conditions such as skin safety, ease of formulation of the strain or fermentation product of the present invention, etc.

[0110] The preparation method, dosage, application method, constituent components, etc. of the quasi-drug can be appropriately selected from conventional techniques known in the technical field.

[0111] In the quasi-pharmaceutical composition of the present invention, the content of the above-mentioned Lactobacillus sakei LGHNH22021 strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain, the crushed material of the above-mentioned strain or a combination thereof can be 0.000001% to 100%, 0.000001% to 90%, 0.000001% to 80%, 0.000001% to 70%, 0.000001% to 60%, 0.000001% to 50%, 0.000001% to 40%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15% based on the total weight of the above-mentioned quasi-pharmaceutical composition. , 0.000001% to 10%, 0.00001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 40%, 0.00001% to 30%, 0.00001% to 20%, 0.00001% to 15%, 0.00001% to 10%, 0.0001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 4 0%, 0.0001% to 30%, 0.0001% to 20%, 0.0001% to 15%, 0.0001% to 10%, 0.001% to 90%, 0.001% to 80%, 0.001% to 70%, 0.001% to 60%, 0.001% to 50%, 0.001% to 40%, 0.001% to 30%, 0.001% to 20%, 0.001% to 15%, 0.001% to 10%, 0.01% to 90%, 0.01% to 80%, 0.01% to 70%, 0.01% to 60%, 0.01% to 50%, 0.0 1% to 40%, 0.01% to 30%, 0.01% to 20%, 0.01% to 15%, 0.01% to 10%, 0.1% to 90%, 0.1% to 80%, 0.1% to 70%, 0.1% to 60%, 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, 0.1% to 15%, 0.1% to 10%, 1% to 90%, 1% to 80%, 1% to 70%, 1% to 60%, 1% to 50%, 1% to 40%, 1% to 30%, 1% to 20%, 1% to 15%, or 1% to 10%, but not limited thereto. For example, the content can be 0.001 to 100%, specifically 0.01 to 30%, but not limited thereto.

[0112] Another aspect of the present invention provides a food composition for improving skin comprising any one or more of the sake Lactobacillus LGHNH22021 strain, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a crushed product of the strain.

[0113] The above-mentioned “Lactobacillus sakei LGHNH22021”, “strain”, “culture”, “culture solution”, “crushed product”, “fermented product”, “extract”, “skin improvement” and the like are as described above.

[0114] Specifically, the food composition is used to improve the skin, and the skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0115] The term "food" of the present invention includes all foods in the usual sense, such as meat, sausage, bread, chocolate, candy, snacks, biscuits, pizza, instant noodles, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, oral liquids, alcoholic beverages, vitamin complexes, health functional foods, etc., and there is no limitation, as long as it can contain the sake lactobacillus LGHNH22021 of the present invention, the culture of the above strain, the fermentation of the above strain, the culture broth of the above strain, the extract of the above strain, and the crushed product of the above strain. In addition, it can be prepared by adding the above strain, the culture, fermentation, culture broth, extract and crushed product of the above strain as the main ingredient to the prepared juice, tea, jelly and fruit juice, etc., and can include the form of pills, powders, granules, tablets, capsules or liquids, etc.

[0116] The above-mentioned health function (property) food (functional food) is the same term as food for special health use (food for special health use, FoSHU), which refers to a food processed so that it can effectively show the medical and medical effects of biological regulation functions in addition to supplying nutrition. Here, "function (property)" refers to the useful effects of health care uses such as regulating nutrients for the structure and function of the human body or physiological effects. The food of the present invention can be prepared by a method commonly used in the art, and during the above preparation, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the dosage form of the above-mentioned food can also be prepared without restriction, as long as it is a dosage form recognized as food. The food composition of the present invention can be prepared in a variety of dosage forms, and unlike general medicines, it uses food as a raw material, so it has the advantages of no side effects that may be caused by long-term use of medicines, and has excellent portability so that the food of the present invention can be taken as an adjuvant for enhancing the immune enhancement effect.

[0117] The above-mentioned health food refers to food that has a positive effect of maintaining or enhancing health compared to general food, and health supplement food refers to food for the purpose of health supplement. Depending on the situation, the terms health functional food, health food, and health supplement food are used interchangeably.

[0118] Specifically, the above-mentioned health functional food is a food prepared by adding any one or more of sake Lactobacillus LGHNH22021, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain and the crushed product of the above-mentioned strain to food materials such as beverages, teas, spices, chewing gum, biscuits, etc., or preparing them into capsules, powders, suspensions, etc., and means that when they are ingested, they will bring specific health effects, but unlike general medicines, they are made from food and therefore have the advantage of not having side effects that may be caused by long-term use of medicines.

[0119] The above composition may further comprise a physiologically acceptable carrier, and the type of the carrier is not particularly limited, and any carrier commonly used in the technical field may be used.

[0120] In addition, the above composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, etc. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folic acid, panthotenic acid, etc. may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), chromium (Cr) may be included. In addition, amino acids such as lysine, tryptophan, cysteine, and valine may be included.

[0121] In addition, the composition may contain food additives such as preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), bactericides (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), etc.), colorants (tar pigments, etc.), colorants (sodium nitrite, sodium nitrite, etc.), bleaching agents (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclamate, saccharin, sodium, etc.), spices (vanillin, lactones, etc.), expanders (alum, D-potassium hydrogen tartrate, etc.), strengtheners, emulsifiers, thickeners (paste), film-forming agents, glue bases, foam inhibitors, solvents, improvers, etc. The additives may be selected according to the type of food and used in appropriate amounts.

[0122] Any one or more of the sake lactobacillus LGHNH22021, the culture of the strain, the fermentation of the strain, the culture solution of the strain, the extract of the strain, and the crushed product of the strain can be directly added or used together with other foods or food ingredients, and can be appropriately used according to conventional methods. The mixing amount of the active ingredient can be appropriately determined according to its purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverages, the food composition of the present invention can be added in an amount of less than 50 parts by weight, specifically less than 20 parts by weight, relative to food or beverage. However, when long-term intake for the purpose of health and hygiene, the content below the above range can be included, and there is no problem in safety, so the active ingredient can also be used in an amount above the above range.

[0123] As an example of the food composition of the present invention, it can be used as a health drink composition, and in this case, like conventional beverages, various flavoring agents or natural carbohydrates can be contained as additional ingredients. The above-mentioned natural carbohydrates can be monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; sugar alcohols such as xylitol, sorbitol, and erythritol. Sweeteners can use natural sweeteners such as thaumatin and stevia extract; synthetic sweeteners such as saccharin and aspartame. Regarding the proportion of the above-mentioned natural carbohydrates, it is generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

[0124] In addition to the above substances, the health drink composition can also contain various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectin acid, pectin salts, alginic acid, alginic salts, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerol, alcohol or carbonating agents, etc. In addition, it can contain the pulp used to prepare natural fruit juice, fruit juice beverage or vegetable beverage. These ingredients can be used alone or in combination. Although the ratio of these additives is not very important, it is generally selected in the range of 0.01 to 0.1 weight parts relative to every 100 weight parts of the composition of the present invention.

[0125] In the food composition of the present invention, based on the gross weight of the food composition, the content of the Lactobacillus sakei LGHNH22021 strain, the culture of the strain, the fermentation product of the strain, the culture solution of the strain, the extract of the strain, the crushed material of the strain or a combination thereof may be 0.000001% to 100%, 0.000001% to 90%, 0.000001% to 80%, 0.000001% to 70%, 0.000001% to 60%, 0.000001% to 50%, 0.000001% to 40%, 0.000001% to 30%, 0.000001% to 20%, 0.000001% to 15% , 0.000001% to 10%, 0.00001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 40%, 0.00001% to 30%, 0.00001% to 20%, 0.00001% to 15%, 0.00001% to 10%, 0.0001% to 90%, 0.00001% to 80%, 0.00001% to 70%, 0.00001% to 60%, 0.00001% to 50%, 0.00001% to 4 0%, 0.0001% to 30%, 0.0001% to 20%, 0.0001% to 15%, 0.0001% to 10%, 0.001% to 90%, 0.001% to 80%, 0.001% to 70%, 0.001% to 60%, 0.001% to 50%, 0.001% to 40%, 0.001% to 30%, 0.001% to 20%, 0.001% to 15%, 0.001% to 10%, 0.01% to 90%, 0.01% to 80%, 0.01% to 70%, 0.01% to 60%, 0.01% to 50%, 0.0 1% to 40%, 0.01% to 30%, 0.01% to 20%, 0.01% to 15%, 0.01% to 10%, 0.1% to 90%, 0.1% to 80%, 0.1% to 70%, 0.1% to 60%, 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, 0.1% to 15%, 0.1% to 10%, 1% to 90%, 1% to 80%, 1% to 70%, 1% to 60%, 1% to 50%, 1% to 40%, 1% to 30%, 1% to 20%, 1% to 15%, or 1% to 10%, but not limited thereto. For example, the content can be 0.001 to 100%, specifically 0.01 to 30%, but not limited thereto.

[0126] Another aspect of the present invention provides a composition for promoting the proliferation of Staphylococcus epidermidis, which is a beneficial skin bacterium, comprising any one or more of the above-mentioned sake Lactobacillus NH22021 strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain, and the crushed product of the above-mentioned strain.

[0127] The composition may be a cosmetic composition, a quasi-drug composition or a food composition, but is not limited thereto.

[0128] The above-mentioned “Lactobacillus sakei LGHNH22021”, “beneficial bacteria to the skin”, and “promoting the proliferation of beneficial bacteria to the skin” are as described above.

[0129] Another aspect of the present invention provides a composition for inhibiting the proliferation of Staphylococcus aureus, which is a skin-harmful bacterium, comprising any one or more of the above-mentioned sake Lactobacillus LGHNH22021 strain, a culture of the above-mentioned strain, a fermentation product of the above-mentioned strain, a culture solution of the above-mentioned strain, an extract of the above-mentioned strain, and a crushed product of the above-mentioned strain.

[0130] The composition may be a cosmetic composition, a quasi-drug composition or a food composition, but is not limited thereto.

[0131] The above-mentioned “Lactobacillus sakei LGHNH22021”, “harmful bacteria to the skin”, and “inhibition of proliferation of harmful bacteria to the skin” are as described above.

[0132] Another aspect of the present invention provides a method for preparing a fermented product, comprising the step of fermenting the above-mentioned sake Lactobacillus LGHNH22021 strain.

[0133] The method for producing the fermented product of the present invention can be appropriately selected from conventional techniques known in the technical field.

[0134] The “Lactobacillus sakei LGHNH22021”, “strain”, “fermentation”, “fermentation product” and the like are as described above.

[0135] Another aspect of the present invention provides a method for preparing a lysate, comprising the step of crushing the above-mentioned Lactobacillus sakei LGHNH22021 strain.

[0136] The method for preparing the solute of the present invention can be appropriately selected from conventional techniques known in the technical field.

[0137] The above-mentioned “Lactobacillus sakei LGHNH22021”, “strain”, “fermentation”, “lysate” and the like are as described above.

[0138] Another object of the present invention is to provide a method for improving skin condition, comprising the step of applying a composition comprising any one or more of the above-mentioned sake Lactobacillus LGHNH22021 strain, the culture of the above-mentioned strain, the fermentation product of the above-mentioned strain, the culture solution of the above-mentioned strain, the extract of the above-mentioned strain, and the crushed product of the above-mentioned strain to the skin of an individual.

[0139] The above-mentioned skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0140] The composition may be a cosmetic composition, a quasi-drug composition or a food composition, but is not limited thereto.

[0141] In the present invention, "individual" may refer to all animals including humans that need or may need one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome. The above-mentioned animals can be not only humans, but also mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, cats, etc. that need to treat similar symptoms, but are not limited thereto.

[0142] The above-mentioned "Sake Lactobacillus LGHNH22021", "strain", "culture", "culture solution", "crushed product", "fermented product", "extract", "skin improvement", "cosmetic composition", "quasi-drug composition" and "food composition" are as described above.

[0143] Another aspect of the present invention provides a composition for improving skin condition comprising any one or more of the above-mentioned Lactobacillus sakei LGHNH22021 strain, a culture of the above-mentioned strain, a fermentation product of the above-mentioned strain, a culture solution of the above-mentioned strain, an extract of the above-mentioned strain, and a crushed product of the above-mentioned strain.

[0144] The above composition may be a cosmetic composition, a quasi-drug composition, or a food composition, but is not limited thereto.

[0145] The above-mentioned skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0146] The above-mentioned "Sake Lactobacillus LGHNH22021", "strain", "culture", "culture solution", "crushed product", "fermented product", "extract", "skin improvement", "cosmetic composition", "quasi-drug composition" and "food composition" are as described above.

[0147] Another aspect of the present invention provides a use of Lactobacillus sakei LGHNH22021 strain deposited with the accession number KCTC 15087BP for improving skin.

[0148] The above-mentioned skin improvement can be one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome, but is not limited thereto.

[0149] Effects of the Invention

[0150] Since the sake Lactobacillus saké LGHNH22021 strain, the culture of the strain, the fermentation product of the strain, the culture solution of the strain, the extract of the strain, or the crushed product of the strain of the present invention has excellent skin improvement effect, it is highly useful as a cosmetic composition, a quasi-drug composition, and a food composition for improving skin. Therefore, various industrial applications such as cosmetics, quasi-drugs, and foods can be expected. BRIEF DESCRIPTION OF THE DRAWINGS

[0151] Figure 1 This is a diagram showing the results of analyzing the effects of the lysate or fermentation product of the Lactobacillus sakei strain LGHNH22021 of the present invention on the enhancement of collagen synthesis. DETAILED DESCRIPTION

[0152] Hereinafter, the present invention is described in more detail by examples. However, these examples are used to illustratively describe the present invention, and the scope of the present invention is not limited to these examples.

[0153] Example 1. Isolation of a novel Lactobacillus sakei strain LGHNH22021 from Achillea millefolium

[0154] A novel strain of Lactobacillus sakei was isolated from Achillea millefolium.

[0155] Specifically, the leaves of Achillea millefolium were washed several times with autoclaved water. Next, 5 g of the leaves were chopped and put into 50 ml of PBS, and the leaves were evenly distributed by vertex. Next, the leaves were diluted with PBS and 100 μl was dispensed into MRS solid medium (BD, 288130) according to the dilution steps, and the solid medium was spread on a flat surface and then cultured at 30°C for 2 days.

[0156] The cultured strain was isolated and identified, confirmed to be Lactobacillus sakei (L. sakei), named Lactobacillus sakei LGHNH22021, and internationally deposited in the BioResource Center of the Korea Institute of Biotechnology (KCTC: Korean Collection for Type Culture), an international depository under the Budapest Treaty, and obtained the deposit number KCTC 15087BP on September 20, 2022.

[0157] The 16s rRNA base sequence of the above strain is shown in SEQ ID NO:1.

[0158] 16s rRNA sequence of Lactobacillus sakei strain LGHNH22021 (SEQ ID NO. 1):

[0159]

[0160] Example 2. Preparation of fermented products and lysates of novel sake Lactobacillus saké LGHNH22021 strain Example 2-1. Preparation of fermented products of novel sake Lactobacillus saké LGHNH22021 strain

[0161] The Lactobacillus sakei LGHNH22021 strain was inoculated into an MRS medium, cultured with shaking at 30° C. and 200 rpm for 4 days, and then centrifuged to collect the supernatant to prepare a fermentation product of the Lactobacillus sakei LGHNH22021 strain.

[0162] The supernatant obtained includes the culture solution of the LGHNH22021 strain of the present invention and the crushed material obtained by crushing the main culture. The above-mentioned centrifuged supernatant is prepared by decomposing organic compounds by the strain, and is therefore equivalent to the fermentation product of the sake Lactobacillus LGHNH22021 strain.

[0163] Example 2-2. Preparation of a lysate (crushed product) of a novel sake Lactobacillus strain LGHNH22021

[0164] The bacterial cells obtained after centrifugation of the culture in the above Example 2-1 were suspended in pure water at 5%, and then treated with ultrasound (amplitude 40, processing time 40 minutes, pulse-ON time 1S, pulse-OFF time 2S) to crush and filter the bacterial cells, and finally prepare a lactobacillus lysate (crushed material).

[0165] Example 2-3. Preparation of fermented product and lysate of standard strain of Lactobacillus sakei (KCTC3603)

[0166] The standard strain of Lactobacillus sakei (KCTC3603) was purchased from the Bioresource Center (KCTC) of Korea Biotechnology Institute. The lysate and fermentation product of the standard strain were prepared by the same method as the lysate and fermentation product of Lactobacillus sakei LGHNH22021 in the above Examples 2-1 and 2-2.

[0167] Example 3. Evaluation of the anti-aging effect of the novel sake Lactobacillus strain LGHNH22021

[0168] In order to verify the anti-aging effect of the new strain, the negative control group (control, DMEM containing 0% serum); the lysate (LS-LG) and fermentation (LS-LGF) of the sake Lactobacillus LGHNH22021 strain of the present invention; the lysate (LS-TS) and fermentation (LS-TSF) of the sake Lactobacillus standard strain (KCTC3603); and culture medium (broth) were treated with human fibroblast HS68 cells according to concentration for 24 hours.

[0169] Next, the collagen synthesis rate was measured using a procollagen type I C-peptide (PIP) kit. The collagen synthesis efficacy of the sample was measured by comparing it with a negative control group (control, DMEM containing 0% serum). The results are shown in Figure 1 , Table 1 and Table 2 below.

[0170] [Table 1]

[0171]

[0172] [Table 2]

[0173]

[0174] like Figure 1 As shown in Tables 1 and 2 below, it was confirmed that the sake Lactobacillus LGHNH22021 strain lysate of the present invention ( Figure 1 A, Table 1) and fermentation products ( Figure 1 B, Table 2) increased collagen synthesis in a concentration-dependent manner, and confirmed that it had a significant collagen synthesis-promoting effect compared with the lysate and fermentation product of the comparative strain KCTC3603.

[0175] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has significant anti-aging, wrinkle improvement and elasticity improvement effects.

[0176] Example 4. Evaluation of the skin regeneration effect of the novel sake Lactobacillus LGHNH22021 strain

[0177] In order to verify the skin regeneration effect of the new strain, the negative control group (control, DMEM containing 0% serum); the lysate (LS-LG) and fermentation (LS-LGF) of the sake Lactobacillus LGHNH22021 strain of the present invention; the lysate (LS-TS) and fermentation (LS-TSF) of the sake Lactobacillus standard strain (KCTC3603); and the culture medium (broth, Broth) were treated with human keratinocytes HaCaT cells according to the concentration for 24 hours.

[0178] Next, CCK-8 solution was added and reacted for 1 hour and 30 minutes, and then absorbance was measured at 460 nm to calculate the proliferation rate of keratinocyte HaCaT cells compared with the negative control group. The results are shown in Tables 3 and 4 below.

[0179] [Table 3]

[0180]

[0181] [Table 4]

[0182]

[0183] As shown in Tables 3 and 4, it was confirmed that the lysate (Table 3) and fermentation product (Table 4) of the sake Lactobacillus strain LGHNH22021 of the present invention increased keratinocyte growth in a concentration-dependent manner, and it was confirmed that the lysate and fermentation product had a significant keratinocyte growth promoting effect compared with the lysate and fermentation product of the comparison strain KCTC3603.

[0184] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has a significant skin regeneration effect.

[0185] Example 5. Evaluation of the skin whitening effect of the novel sake Lactobacillus strain LGHNH22021

[0186] In order to verify the skin whitening effect of the new strain, the negative control group (control, DMEM containing 0% serum); the lysate (LS-LG) and fermentation product (LS-LGF) of the sake Lactobacillus LGHNH22021 strain of the present invention; the lysate (LS-TS) and fermentation product (LS-TSF) of the sake Lactobacillus standard strain (KCTC3603); and the culture medium (broth, Broth) were treated in mouse melanoma cell line B16F1 according to the concentration, and then cultured for 72 hours.

[0187] Next, the cells were collected and centrifuged to discard the supernatant, and then 100ul of 1N NaOH / 10% DMSO solution was placed and dissolved at 80 degrees for 20 minutes. Next, the absorbance was measured at 400nm with a microplate reader, and the melanin synthesis amount was calculated using a melanin standard curve, and then expressed as a ratio compared to the negative control group. The results are shown in Tables 5 and 6 below.

[0188] [Table 5]

[0189]

[0190] [Table 6]

[0191]

[0192] As shown in Tables 5 and 6, it was confirmed that the lysate (Table 5) and fermentation product (Table 6) of the sake Lactobacillus strain LGHNH22021 of the present invention inhibited melanin synthesis in a concentration-dependent manner, and it was confirmed that the lysate and fermentation product had a significant melanin synthesis inhibitory effect compared with the lysate and fermentation product of the comparison strain KCTC3603.

[0193] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has a significant whitening effect.

[0194] Example 6. Evaluation of the skin moisturizing effect of the novel sake Lactobacillus LGHNH22021 strain

[0195] In order to verify the skin moisturizing effect of the new strain, the negative control group (control, DMEM containing 0% serum); the lysate (LS-LG) and fermentation product (LS-LGF) of the sake Lactobacillus LGHNH22021 strain of the present invention; the lysate (LS-TS) and fermentation product (LS-TSF) of the sake Lactobacillus standard strain (KCTC3603); and the culture medium (broth, Broth) were treated in human keratinocytes HaCaT for 24 hours according to the concentration, and then RNA was extracted.

[0196] Next, 1 μg of RNA was used to synthesize cDNA, and then the expression of hyaluronan synthase HAS2 and moisture retention-related gene aquaporin 3 (AQP3) was confirmed by real time PCR. The results are shown in Table 7 (HAS2) and Table 8 (AQP3) below.

[0197] [Table 7]

[0198]

[0199] [Table 8]

[0200]

[0201] As shown in Table 7, it was confirmed that the lysate of the Lactobacillus sake LGHNH22021 strain of the present invention increased the expression of hyaluronan synthase HAS2 in a concentration-dependent manner compared to the conventionally known Lactobacillus sake comparison strain KCTC3603. That is, it was confirmed that compared to the lysate of the comparison strain KCTC3603, there was a significant effect of increasing hyaluronic acid production.

[0202] In addition, as shown in Table 8, it was confirmed that the fermentation product of the Lactobacillus sake LGHNH22021 strain of the present invention increased the expression of the water transport membrane protein, aquaporin 3, in a concentration-dependent manner compared to the conventionally known sake Lactobacillus sake comparison strain KCTC3603. That is, it was confirmed that the fermentation product of the comparison strain KCTC3603 had a significant effect of increasing the expression of aquaporin 3.

[0203] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has a significant moisturizing effect. Example 7 Evaluation of the skin soothing and anti-inflammatory effects of the novel sake Lactobacillus LGHNH22021 strain

[0204] In order to verify the skin sedative and anti-inflammatory effects of the new strain, the negative control group (control, serum free culture medium); the lysate (LS-LG) and fermentation product (LS-LGF) of the sake Lactobacillus LGHNH22021 strain of the present invention; the lysate (LS-TS) and fermentation product (LS-TSF) of the sake Lactobacillus standard strain (KCTC3603); and the culture medium (broth, Broth) were pretreated in mouse macrophage cell line Raw264.7 cells for 1 hour according to the concentration, and then treated with 0.5 ppm LPS for 24 hours.

[0205] Next, 4% Griess reagent was added to each cell culture solution, and then reacted at room temperature for 10 minutes. Afterwards, the absorbance was measured at 540 nm using a microplate reader. The NO generation was calculated using the NO standard curve, and then the NO generation inhibition rate compared to the negative control group was displayed. The results are shown in Tables 9 and 10 below.

[0206] [Table 9]

[0207]

[0208] [Table 10]

[0209]

[0210] As shown in Tables 9 and 10, it was confirmed that the lysate (Table 9) and fermentation product (Table 10) of the sake Lactobacillus strain LGHNH22021 of the present invention inhibited NO production in a concentration-dependent manner, and it was confirmed that the lysate and fermentation product had a significant NO production inhibitory effect compared with the lysate and fermentation product of the comparison strain KCTC3603.

[0211] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has a significant skin soothing and inflammation improving effect.

[0212] Example 8. Evaluation of the Microbiome Improvement Effect of the Novel Sake Lactobacillus LGHNH22021 Strain

[0213] The microbiome-improving effect (in vitro prebiotic ability) was confirmed using the fermentation product of the novel strain, and the microbiome-improving effect was compared by measuring the changes in the growth rates of beneficial and harmful bacteria caused by the fermentation products of the control group and the comparison strain (fermentation product of the sake Lactobacillus standard strain (KCTC3603)).

[0214] Among the bacteria resident on the skin, Staphylococcus epidermidis (S. epidermidis) was selected as a representative beneficial bacteria, and Staphylococcus aureus (S. aureus) was selected as a representative harmful bacteria.

[0215] Specifically, a sterile liquid culture medium is prepared using a commercial TSB (Tryptic soy broth) culture medium. Staphylococcus epidermidis glycerol stock or Staphylococcus aureus glycerol stock is inoculated into a sterile liquid TSB culture medium 1-2 times and pre-cultured at 30-37°C for 6-18 hours. A negative control group (control, serum free culture solution); a fermentation product (LS-LGF) of the sake lactobacillus LGHNH22021 strain of the present invention; a fermentation product (LS-TSF) of the sake lactobacillus standard strain (KCTC3603); and a culture medium (broth, Broth) are added to a sterile liquid TSB culture medium at 1%, and the above pre-culture solution is inoculated and cultured at 30-37°C for 6-18 hours. After culturing, the absorbance just after inoculation and the absorbance during the stationary phase are measured, and the ratio of their values ​​(growth rate) is used as a statistical variable. The growth rate of the treated sample experimental group was compared with that of the untreated sample control group to determine whether the sample had a significant effect on the growth of the bacteria. The evaluation was performed 3-6 times or more, and the average value, standard deviation, etc. of the growth rate of the multiple samples were calculated, and then the significance was verified by t-test. The results are shown in Tables 11 and 12 below.

[0216] [Table 11]

[0217]

[0218] [Table 12]

[0219]

[0220] As shown in Table 11, it was confirmed that the fermented product of the Lactobacillus sake LGHNH22021 strain of the present invention promoted the growth of Staphylococcus epidermidis, which is a beneficial bacteria, compared with the conventionally known Lactobacillus sake comparison strain KCTC3603. That is, it was confirmed that it had a significant effect of promoting the proliferation of skin beneficial bacteria compared with the lysate of the comparison strain KCTC3603.

[0221] In addition, as shown in Table 12, it was confirmed that the fermented product of the Lactobacillus sake LGHNH22021 strain of the present invention inhibited the growth of Staphylococcus aureus, which is a harmful bacterium, compared with the conventionally known Lactobacillus sake comparison strain KCTC3603. That is, it was confirmed that the fermented product of the Lactobacillus sake LGHNH22021 strain of the present invention had a significant effect of inhibiting the proliferation of harmful skin bacteria compared with the lysate of the comparison strain KCTC3603.

[0222] Based on this, it was confirmed that the sake Lactobacillus LGHNH22021 strain of the present invention has a significant microbiome-improving effect (skin flora-improving effect).

[0223] Based on the above description, a person skilled in the art of the present application can understand that the present application can also be implemented in other specific ways without changing the technical idea or necessary features of the present application. In this regard, it should be understood that the embodiments described above are exemplary and non-restrictive in all aspects. The scope of the present application should be interpreted as including the meaning and scope of the claims described later and all changes or deformations derived from their equivalent concepts, rather than just the above detailed description.

[0224] [Accession number]

[0225] Name of depository institution: Korea Type Culture Collection

[0226] Accession number: KCTC 15087BP

[0227] Date of preservation: September 20, 2022.

Claims

1. A Lactobacillus sakei LGHNH22021 strain, which is deposited under the accession number KCTC15087BP.

2. The strain according to claim 1, wherein The strain has one or more characteristics selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome.

3. The strain according to claim 1, wherein The above strain is derived from yarrow (Achilleamillefolium).

4. A cosmetic composition for improving skin, comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a pulverized product of the strain.

5. The cosmetic composition according to claim 4, wherein The above-mentioned skin improvement is one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome.

6. A quasi-pharmaceutical composition for improving skin, comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a pulverized product of the strain.

7. A food composition for improving skin, comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a pulverized product of the strain.

8. A composition for promoting the proliferation of Staphylococcus epidermidis, which is a skin-friendly bacterium, comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a crushed product of the strain.

9. A composition for inhibiting the proliferation of Staphylococcus aureus, which is a skin-harming bacterium, comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a crushed product of the strain.

10. A method for preparing a fermentation product, comprising the step of fermenting the strain according to claim 1.

11. A method for preparing a lysate, comprising the step of crushing the strain according to claim 1.

12. Use of Lactobacillus sakei LGHNH22021 strain deposited with the accession number KCTC 15087BP for improving skin.

13. Use of the strain according to claim 12 for improving skin, wherein: The above-mentioned skin improvement is one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome.

14. Use of a cosmetic composition for improving skin comprising any one or more of the strain according to claim 1, a culture of the strain, a fermentation product of the strain, a culture solution of the strain, an extract of the strain, and a pulverized product of the strain.

15. Use of the cosmetic composition for improving skin according to claim 14, wherein: The above-mentioned skin improvement is one or more selected from the group consisting of anti-aging, wrinkle improvement, elasticity improvement, skin regeneration, skin whitening, skin moisturizing, skin calming, anti-inflammation and improvement of skin microbiome.

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