Composition for synergistically preventing and treating banana wilt and application thereof
By using the compounds of 8-hydroxylinoline and 6-pentomy-2H-Pyra-2-ketone and other compounds, combined with EDDHA-FE, it solves the problem of effectively preventing banana disease in existing technologies. The effective inhibitory of banana wilt and the healthy growth of plants.
Patent Information
- Application Number
- CN202411517309.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-29
- Publication Date
- 2025-01-28
AI Technical Summary
The prior art is difficult to effectively control the spread of Fusarium oxysporus spores and the prevention and treatment of banana blight, especially in plants.
A composition with a collaborative prevention and treatment of banana, including 8-Hydroxyquinoline (8HQ) and 6-pentomy-2H-pyra-2-ketone (6-pentyl-Pyran-2- One, 6-pp), and add EDDHA-FE under appropriate circumstances to achieve the suppression of banana withered disease.
This composition has significantly improved the prevention and treatment of banana witness through a clear bacteriostatic mechanism and clear role, and is friendly and easy to obtain.
Smart Images
Figure CN119366522A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of agriculture, and particularly relates to a composition for synergistically preventing and controlling banana wilt disease and application thereof. Background Art
[0002] Fusarium oxysporum is the main cause of banana wilt, of which races 1 and 4 pose a serious threat to global banana production (Siddhesh, International Journal of Pest Management, 2015, 61(3):250-263). Its soil-borne nature makes it very difficult to control or even eliminate Fusarium oxysporum (Carvalhais, Frontier in plant science, 2019, 10). As the disease progresses, the leaves of the plant begin to turn yellow and gradually wilt. As the disease spreads further, the leaves will fall off and form a circle around the pseudostem. The new leaves will have wrinkled edges (Berg, Molecular Plant Pathology, 2010, 8(3)). Once banana plants are infected with Fusarium oxysporum, there is currently no effective control measure. This difficulty is due to the fact that Fusarium oxysporum can produce a large number of spores, which can survive in the soil for up to 30 years (Ploetz, Plant Health Prog, 2000, 10: 1-7). Existing fungicides and fumigation methods cannot effectively control the spread of Fusarium oxysporum spores (Ploetz, Plant Health Progress, 2005).
[0003] Further screening of fungicides effective against Fusarium oxysporum, some of which come from known chemical reagents, and the other part comes from metabolites in biocontrol bacteria. Mao et al. (Mao Haiqing, Tropical Agricultural Science, 2019, 39(8): 72-76.) used Schiff base compounds to study their inhibitory effects on banana wilt pathogens in a plate confrontation experiment. Zhu et al. (Zhu, Communications Biology, 2023, 6: 27) studied the use of Streptomyces Sm4-1986 and Indian pear-shaped spores to synergistically control banana wilt. Most of the research on the antibacterial effect of compounds is concentrated in the plate confrontation experiment stage, and less application on plants. The application of biocontrol bacteria has certain advantages in the ecological environment, but its stability fluctuates due to factors such as natural conditions. Combined with the antibacterial mechanism of biocontrol bacteria, it is urgent to develop a compound control method with targeted treatment. Summary of the invention
[0004] Technical problems to be solved: In view of the above technical problems, the present invention provides a composition for synergistically preventing and controlling banana wilt disease and its application, which can effectively solve the shortcomings of the above compounds in that the antibacterial effect is less applied to plants.
[0005] Technical solution: In a first aspect, the present invention provides a composition for synergistically preventing and controlling banana wilt disease, comprising 8-hydroxyquinoline (8HQ) and 6-pentyl-2H-pyran-2-one (6-PP).
[0006] Preferably, the mass ratio of 8-hydroxyquinoline to 6-pentyl-2H-pyran-2-one is 1:32.
[0007] Preferably, the composition further comprises EDDHA-Fe.
[0008] Furthermore, the mass ratio of the EDDHA-Fe to 8-hydroxyquinoline is 1:3.
[0009] In a second aspect, the present invention provides use of the composition described in the first aspect in preparing a product for synergistically controlling banana wilt.
[0010] Beneficial effects: The present invention provides a composition for synergistically preventing and controlling banana wilt disease, which has the characteristics of clear antibacterial mechanism, clear action point, easy acquisition of single compounds, and environmental friendliness. 6-pentyl-2H-pyran-2-one is the main antibacterial component. When analyzing the physical and chemical properties of the soil of banana wilt disease-infected plants and healthy plants, it was found that the Fe content in the rhizosphere of the soil was negatively correlated with its incidence rate. Therefore, the iron chelator 8-hydroxyquinoline was used for combined application; during the application process, after 8HQ chelated iron, the pathogen failed to grow, but it also affected the growth of the plant; in the subsequent selection, EDDHA-Fe was added to supplement the plant iron agent, and the pathogen failed to effectively utilize EDDHA-Fe, achieving the effect of three-dimensional prevention and control. 8HQ is inexpensive and practical; EDDHA chelated iron can also be directly selected, but due to the high price of EDDHA, its application lacks an economic basis. BRIEF DESCRIPTION OF THE DRAWINGS
[0011] Figure 1 The three-dimensional response surface diagram (A) and interaction curve diagram (B) of the interaction between different concentrations of 8-hydroxyquinoline (8HQ) and different concentrations of 6-pentyl-2H-pyran-2-one (6-PP), where X1 is 25-125 μM 8HQ and X2 is 0.6-2.4 mM 6-PP; Figure 2Schematic diagram of the antibacterial effect of applying different compounds in one embodiment of the present invention, wherein A is a control, B is the application of 74 µM 8HQ and 2.4 mM 6-PP, C is the application of 74 µM 8HQ, and D is the application of 2.4 mM 6PP; Figure 3 This is a graph showing the effect of 8HQ on inhibiting the growth of banana wilt pathogen (FOC4) by chelating iron in one embodiment of the present invention, wherein A is the control, B is 200 µM 8HQ, C is 200 µM 8HQ and 70 µM FeCl3, and D is the effect of 8HQ chelating different concentrations of FeCl3 on the disease of banana seedlings, which are 0, 0.7, 7, 70, 700 and 7000 µM FeCl3 from left to right; Figure 4 The figures are the statistical data of disease index of banana seedlings after different treatments (A) and the disease conditions of banana seedlings after different treatments (B) in one embodiment of the present invention, wherein CK is a control, treated with 200 mL of sterile water; FOC4 is a pathogen treatment, treated with 200 mL of spore solution of Fusarium oxysporum (spore concentration is 1×10 7 T1: 200 mL of Fusarium oxysporum spore solution (spore concentration of 1×10 7 / mL), 10 mL 74 µM 8HQ and 10 mL 2.4 mM 6-PP were used for root irrigation; T2: 200 mL of Fusarium oxysporum spore solution (spore concentration was 1×10 7 / mL), 10 mL 74 µM 8HQ and 10 mL 2.4 mM 6-PP were used for root irrigation, and 70 µM EDDHA-Fe was applied for foliar spraying. DETAILED DESCRIPTION
[0012] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments: Example 1: 8HQ and 6-PP have a synergistic effect in inhibiting the growth of banana wilt pathogens The compound experiment was conducted using 25-125 μM 8HQ and 0.6-2.4 mM 6-PP. The results are as follows: Figure 1 As shown in A and B: The optimal application concentrations were 74 µM 8HQ and 2.4 mM 6-PP; The antibacterial effect of different compounds was tested. The results are as follows Figure 2 As shown: A is the control, B is the application of 74 µM 8HQ and 2.4 mM 6-PP, C is the application of 74 µM 8HQ, and D is the application of 2.4 mM 6PP. It can be seen that 74 µM 8HQ and 2.4 mM 6-PP have stronger antibacterial effects than a single applied compound, and enhance each other's antibacterial properties against banana wilt.
[0013] Example 2: Antibacterial mechanism of 8HQ 8HQ Figure 3 The antibacterial mechanism of AC was demonstrated. Figure 3 A is a control experiment. Figure 3 B: No growth of pathogens after adding 8HQ. Figure 3 In C, when Fe was added to the culture medium with 8HQ, the pathogen resumed growth, indicating that 8HQ inhibited the growth of pathogens by chelating the iron in the culture medium. Figure 3 In D, adding 8HQ to the culture medium first and then supplementing with different concentrations of Fe can restore the growth of the plant seedlings. This shows that the Fe chelated by 8HQ cannot be used by the plant, and supplementing with excess Fe can restore the plant growth. Therefore, adding Fe to the environment further restored the growth of the banana plant.
[0014] Example 3: Control of banana wilt using 74 µM 8HQ, 2.4 mM 6-PP and 70 µM EDDHA-Fe After getting the banana plant seedlings in bags, plant them in small pots with 200 g of sterile soil. When they grow to the five-leaf and one-heart stage, four treatments were set up: CK: add 200 mL of sterile water, FOC4: add 100 mL of Fusarium oxysporum spore solution and 100 mL of sterile water, T1: add 100 mL of Fusarium oxysporum spore solution, 30 mL of 8HQ and 30 mL of 6-PP and fill up to 200 mL with sterile water, T2: add 100 mL of Fusarium oxysporum spore solution, 30 mL of 8HQ, 30 mL of 6PP and 10 mL of EDDHA-Fe and fill up to 200 mL with sterile water; The results are as follows Figure 4 As shown in the figure: 4 µM 8HQ, 2.4 mM 6-PP and 70 µM EDDHA-Fe can effectively reduce the incidence index and morbidity of banana wilt. Among them, EDDHA-Fe plant pathogens cannot use it, but banana plants can absorb it. Therefore, this example uses cheap EDDHA-Fe, 8HQ and 6-PP to effectively prevent and control banana wilt.
[0015] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A composition for synergistically preventing and treating banana wilt, characterized in that: These include 8-hydroxyquinoline and 6-pentyl-2H-pyran-2-one.
2. The composition for synergistically preventing and controlling banana wilt according to claim 1, characterized in that: The mass ratio of the 8-hydroxyquinoline to the 6-pentyl-2H-pyran-2-one is 1:
32.
3. The composition for synergistically preventing and controlling banana wilt according to claim 1, characterized in that: Also includes EDDHA-Fe.
4. A composition for synergistically controlling banana wilt according to claim 3, characterized in that: The mass ratio of the EDDHA-Fe to 8-hydroxyquinoline is 1:
3.
5. Use of the composition according to claim 1 or 3 in preparing a product for synergistically preventing and controlling banana wilt.