Composite bactericidal composition containing tetramycin and picoxystrobin, bactericide and application

Abstract

The invention discloses a compound bactericidal composition containing tetramycin and picoxystrobin, a bactericide and application, and belongs to the technical field of pesticide compounding. According to the application, indoor toxicity determination is carried out on tetramycin, polyoxin, zhongshengmycin, picoxystrobin, pyraclostrobin and thiophanate methyl. Results show that the lowest concentration of the biological bactericide in inhibition of colletotrichum gloeosporioides is tetramycin, and the lowest concentration of the biological bactericide in inhibition of colletotrichum gloeosporioides is picoxystrobin. The ratio of tetramycin to picoxystrobin is measured and screened under an in-vitro condition, a result shows that when the mass ratio of tetramycin to picoxystrobin is 1: 10, a very strong synergistic effect is achieved, a field test result shows that when the field indirect bacterium concentration is 6 * 10 < 4 > cfu / ml, the highest prevention effect on grape anthracnose bacteria is 86.29%, a basis is provided for effectively preventing and treating grape anthracnose, and the application prospect is broad. And a new choice is provided for preventing and treating grape anthracnose.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and in particular relates to a composite fungicide composition containing tetramycin and picoxystrobin, a fungicide and application thereof. Background Art

[0002] Grapes are prone to many diseases and pests in actual production, such as bitter rot, black rot, and Botrytis bunch rot, among which grape anthracnose is the most serious. Grape anthracnose mainly occurs during the fruit ripening period, and the rotten fruit is usually covered with orange conidia. Grape ripe rot was first discovered and reported in the United States in 1891, and now occurs in most grape-growing areas in my country. Relevant studies at home and abroad have shown that a variety of pathogens can cause grape ripe rot, among which Colletotrichum gloeosporioides and C. acutatum are the most reported, and C. boninense and C. orchidearum species complexes are occasionally involved. The pathogens of grape ripe rot in my country are mainly C. gloeosporioides complex, which mainly includes C. gloeosporioides, C. fructicola and C. viniferum. There are at least four species of grape anthracnose (Colletotrichum spp.) in Jurong City, including C. gloeosporioides, C. aenigma, C. fructicola and C. viniferum. The prevention and control of grape ripe rot at home and abroad is mainly based on chemical control. The main agents used for the prevention and control of grape ripe rot in production are carbendazim and methyl thiophanate of benzimidazoles (Ben); azoxystrobin and pyraclostrobin of methoxyacrylates (QoIs); difenoconazole and tebuconazole of sterol demethylation inhibitors (DMI). The drug resistance management technology is mainly substitution and rotation technology, that is, after the pathogen develops resistance to one agent, another agent with a different mechanism of action is used instead or in rotation. However, the fungicides in the prior art generally have defects such as poor antibacterial efficacy and inability to achieve long-term antibacterial effect. Summary of the invention

[0003] In view of the above technical problems, the present invention provides a composite bactericidal composition, a bactericide and applications containing tetramycin and picoxystrobin. The composite bactericidal composition and the bactericide provided by the present invention have the advantages of convenient preparation and good control effect.

[0004] The technical solution provided by the present invention is as follows:

[0005] A composite bactericidal composition containing tetramycin and picoxystrobin, wherein the composite bactericidal composition comprises tetramycin and picoxystrobin, and the mass ratio of tetramycin to picoxystrobin is 1:8-12.

[0006] Furthermore, the mass ratio of tetramycin to picoxystrobin is 1:10.

[0007] Furthermore, the concentration of tetramycin is 0.125-4 mg / L, and the concentration of picoxystrobin is 0.003-1 mg / L.

[0008] The present invention also provides a method for preparing the composite fungicide composition containing tetramycin and picoxystrobin, wherein tetramycin and picoxystrobin are compounded into a suspension, a suspoemulsion or a dispersible oil suspension according to a mass ratio of 1:10.

[0009] The present invention also provides a bactericide, comprising the composite bactericidal composition.

[0010] Furthermore, the bactericide also includes alkyl aryl polyoxyethylene ether, wetting and penetrating agent F, Tween 80, silicone oil, polyvinyl alcohol, propylene glycol and deionized water.

[0011] Furthermore, the fungicide is a suspoemulsion.

[0012] The present invention also provides application of the composite fungicide composition or the fungicide in the prevention and treatment of grape anthracnose.

[0013] Furthermore, the above-mentioned composite fungicide composition or the above-mentioned fungicide is sprayed on grapes.

[0014] Furthermore, the spray treatment is performed during the young grape fruit stage.

[0015] Beneficial Effects

[0016] In this application, grape anthracnose (Colletotrichum viniferum) was used as the test bacteria, and the mycelium growth rate method was used to conduct indoor toxicity tests on tetramycin, polyoxin, sinocycline, picoxystrobin, pyraclostrobin and methyl thiophanate. The results showed that among the above-mentioned biological fungicides, tetramycin had the lowest inhibition concentration on grape anthracnose, and its EC50 was 0.6789 mg / L. Among the above-mentioned chemical fungicides, picoxystrobin had the lowest inhibition concentration on grape anthracnose, and its EC50 was 0.0464 mg / L. The ratio of tetramycin and picoxystrobin was screened under in vitro conditions. The results showed that when the mass ratio of tetramycin to picoxystrobin was 1:10, the EC50 was only 0.0207 mg / L, and the SR value was 2.45, which had a strong synergistic effect. The field test results showed that when the in vitro bacterial concentration was 6*10 4 cfu / ml, the highest protection effect against grape anthracnose (Colletotrichum viniferum) was 86.29%, which provided a basis for the effective prevention and control of grape anthracnose and a new choice for the prevention and control of grape anthracnose.

[0017] The present invention relates to a novel tetramycin and picoxystrobin composite fungicide composition, which is particularly suitable for preventing and treating grape anthracnose. The composition not only has a significant fungicide effect, but also can overcome and delay the drug resistance of pathogens, improve the yield and quality of grapes, reduce the cost of pesticide use, and has less impact on the environment and human health.

[0018] Grape anthracnose is one of the common diseases during the growth period of grapes, which has a serious impact on the yield and quality of grapes. Tetramycin, as a broad-spectrum fungicide, has the characteristics of a wide antibacterial spectrum and good fungicide effect, while picoxystrobin is a highly effective systemic fungicide that can quickly penetrate plant tissues and reach the diseased parts to exert a fungicide effect. The combined use of tetramycin and picoxystrobin can give full play to the synergistic effect of the two, with strong targeting and high efficiency in the prevention and control of grape anthracnose.

[0019] Studies have shown that in the early stage of grape anthracnose, the composite fungicide composition of the present invention can be used for prevention and treatment, which can quickly inhibit the growth and reproduction of pathogens and effectively reduce the spread and diffusion of the disease. Compared with the use of tetramycin or picoxystrobin alone, the control effect of the composite fungicide composition is significantly improved, and the incidence and disease index of grape anthracnose are significantly reduced.

[0020] Grape anthracnose pathogens are prone to develop drug resistance when exposed to a single fungicide for a long time, resulting in reduced control effects. The composite fungicide composition of the present invention acts on pathogens through different fungicidal mechanisms, reducing the possibility of pathogens developing drug resistance to a single fungicide.

[0021] Studies have shown that the development of drug resistance of grape anthracnose pathogens is significantly delayed when the composite fungicide composition is used continuously. Compared with the use of tetramycin or picoxystrobin alone, the composite fungicide composition can maintain a high-efficiency control effect for a longer period of time, extend the service life of the fungicide, and reduce the difficulty and cost of disease control.

[0022] Grape anthracnose not only affects the yield of grapes, but also reduces the quality of grapes, such as affecting the appearance, taste and nutritional value of grapes. Using the composite fungicide composition of the present invention for prevention and treatment can effectively reduce the damage of the disease to grapes, protect the healthy growth of grapes, and thus improve the yield and quality of grapes.

[0023] Studies have shown that when the composite fungicide composition is applied, the yield of grapes is significantly increased compared to the control group without fungicide application. At the same time, the quality of grapes has also been significantly improved, with neat bunches, plump grapes, bright colors and better taste. In addition, the composite fungicide composition can also promote the growth and development of grapes, improve the stress resistance and nutritional value of grapes, and provide strong support for the sustainable development of the grape industry.

[0024] Since the composite fungicide composition of the present invention has a significant control effect, it can reduce the number of times and the amount of pesticides used, thereby reducing the cost of pesticide use. Compared with the use of tetramycin or picoxystrobin alone, the cost of the composite fungicide composition is reduced by more than about 15%. In addition, the preparation process of the composite fungicide composition is simple, the raw materials are easily available, and the production cost is low. Therefore, in the prevention and control of grape anthracnose, the use of the composite fungicide composition can not only improve the control effect, but also reduce the cost of pesticide use, bringing greater economic benefits to grape growers.

[0025] Tetramycin and picoxystrobin are both low-toxic and low-residue pesticides with little impact on the environment and human health. During the application process, the composite fungicide composition can be rapidly degraded and will not cause long-term pollution to the environment. At the same time, the residual amount of the composite fungicide composition is low and will not cause harm to human health.

[0026] In addition, the composite fungicide composition of the present invention also has good environmental compatibility, and can coexist harmoniously with microorganisms, plants and other organisms in the ecological environment such as soil and water sources, without causing damage to the ecological environment. Therefore, in the prevention and treatment of grape anthracnose, the use of the composite fungicide composition can not only improve the prevention and treatment effect, but also protect the ecological environment and human health, and realize green, environmentally friendly and sustainable agricultural production.

[0027] In summary, the tetramycin and picoxystrobin composite fungicide composition of the present invention has significant beneficial effects in preventing and treating grape anthracnose. The composition not only has the advantages of high sterilization, overcoming and delaying bacterial resistance, improving grape yield and quality, and reducing the cost of pesticide use, but also has little impact on the environment and human health. Therefore, the composite fungicide composition has broad application prospects and economic benefits in the prevention and treatment of grape anthracnose, and is of great significance for ensuring the healthy development of my country's grape industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a comparison diagram of grapes treated differently in Example 3. DETAILED DESCRIPTION

[0029] Test strain: Grape anthracnose pathogen Colletotrichum viniferum

[0030] It was collected from Dingzhuang Village, Maoshan Town, Jurong City, Jiangsu Province, and isolated, identified and preserved by the Ecological Agriculture Research Laboratory of Zhenjiang Agricultural Science Research Institute in the hilly area of ​​Jiangsu Province.

[0031] The strains were stored in a -20°C refrigerator using filter paper.

[0032] After the filter paper strain was activated on PDA medium in the laboratory, it was cultured in a 25°C incubator for 7 days, and a 4mm diameter bacterial cake was taken from the edge of the colony for experimental determination.

[0033] Example 1

[0034] Indoor toxicity test of fungicides against grape anthracnose

[0035] Preparation of mother solution: prepare tetramycin, polyoxin, sinomenine, picoxystrobin, pyraclostrobin and thiophanate-methyl into 10000ml / L mother solution respectively with sterile pure water.

[0036] The concentration design of each single dose of medicine is shown in Table 1 below, and all of them are prepared and used immediately.

[0037] Table 1 Design of different concentrations of 6 fungicides

[0038] Pharmacy Concentration (mg / L) Tetramycin 4，2，1，0.5，0.25，0.125 Polymycin 4，2，1，0.5，0.25，0.125 Zhongshengmycin 4，2，1，0.5，0.25，0.125 Picoxystrobin 1，0.3，0.1，0.03，0.01，0.003 Pyraclostrobin 10，3，1，0.3，0.1，0.03 Thiophanate methyl 10，3，1，0.3，0.1，0.03

[0039] Table 2 shows different concentration designs of tetramycin alone, prothioconazole alone, and tetramycin and picoxystrobin combined, all of which are prepared and used immediately.

[0040] Table 2 Different concentrations of tetramycin alone, picoxystrobin alone, and tetramycin and picoxystrobin combined

[0041] Pharmacy Concentration (mg / L) Tetramycin 4，2，1，0.5，0.25，0.125 Picoxystrobin 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 1:10 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 1:7 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 1:3 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 1:1 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 3:1 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 7:1 1，0.3，0.1，0.03，0.01，0.003 Tetramycin-picoxystrobin 10:1 1，0.3，0.1，0.03，0.01，0.003

[0042] The compounding ratio of the above tetramycin and picoxystrobin is 1-10:10-1 as the mass ratio.

[0043] After culturing for 7 days at 25°C, the colony diameters of each treatment were measured using the cross method. PDA medium without fungicide was used as the control treatment group, and each treatment was repeated 4 times. The drug inhibition rate corresponding to each drug concentration was calculated, and the effective median concentration (EC 50 ), and the toxicity regression equation, relative coefficient and 95% confidence interval of the agent were obtained.

[0044] The results of the indoor toxicity test of 6 fungicides against grape anthracnose showed (Table 4) that among the chemical agents, picoxystrobin had the lowest concentration of inhibition against grape anthracnose, and among the biological agents, tetramycin had the lowest concentration of inhibition against grape anthracnose. The EC50 of the 6 single agents were: tetramycin (0.6789 mg / L) > polyoxin (1.3081 mg / L) > zhongshengmycin (1.6809 mg / L); picoxystrobin (0.0464 mg / L) > pyraclostrobin (1.1634 mg / L) > thiophanate-methyl (2.8989 mg / L). Compared with other biological agents and chemical agents, tetramycin and picoxystrobin have good antibacterial activity.

[0045] Table 3 Inhibition rate of mycelial growth of grape anthracnose by six fungicides

[0046]

[0047] Table 4 Inhibitory effects of six fungicides on the mycelial growth of grape anthracnose

[0048]

[0049] It can be seen from Table 5 that when the concentration of tetramycin is 0.125-4 mg / L, the inhibition rate of mycelial growth of grape anthracnose is 8.29-93.09%; when the concentration of picoxystrobin is 0.003-1 mg / L, the inhibition rate of mycelial growth of grape anthracnose is 21.20-83.41%; when the ratio of tetramycin to picoxystrobin is 1:10, 1:7, 1:3 and 1:1 respectively, the inhibition rate of mycelial growth of grape anthracnose is 21.20-83.41%. The inhibition rates of anthracnose fungus mycelium growth were 30.41-90.32%, 20.74-86.64%, 16.59-78.80% and 9.68-75.58% respectively; when the ratios of tetramycin to picoxystrobin were 10:1, 7:1 and 3:1 respectively, the inhibition rates of grape anthracnose fungus mycelium growth were 0-53.46%, 1.84-58.53% and 9.68-67.74% respectively.

[0050] Example 2

[0051] EC in fungicide activity assay50 The synergistic coefficient is calculated by the ratio of the single agent in the mixture. Eob represents the EC of the mixture 50 The actual efficacy of Eth is the theoretical efficacy of the mixture, and the calculation formula is as follows:

[0052] Mixture EC 50 of

[0053] In the formula, A and B represent the single dose of each fungicide, and a and b represent the proportion of each single dose component in the mixture.

[0054] Synergy coefficient (SR) = Eth / Eob

[0055] SR>1.5 is a synergistic effect; 0.5≤SR≤1.5 is an additive effect; SR<0.5 is an antagonistic effect.

[0056] Tetramycin and picoxystrobin were compounded. It can be seen from Table 6 that when the compounding ratio was 1:10, the maximum SR value was 2.45, which had a synergistic effect. 50 The ratio of tetramycin to picoxystrobin is 0.0207 mg / L. Only when the compound ratio is 1:10, it has a synergistic effect, and other compound ratios have an additive effect. Therefore, we choose the optimal compound ratio of tetramycin to picoxystrobin as 1:10.

[0057] Table 5 Inhibition rate of mycelial growth of grape anthracnose by tetramycin, picoxystrobin and their different ratios

[0058]

[0059]

[0060] Table 6 Screening of the best ratio of tetramycin and picoxystrobin against grape anthracnose

[0061]

[0062]

[0063] Example 3

[0064] Application of fungicides in the prevention and control of grape anthracnose

[0065] Implementation time and location: This embodiment will be carried out in Xia Dian Village, Huayang Town, Jurong City from May to July 2024.

[0066] Tetramycin and picoxystrobin were compounded into suspension, suspoemulsion and dispersible oil suspension in a mass ratio of 1:10, and inoculated in the rain-sheltered vineyard of Xiadian Village, Jurong City (the grape variety was Xiahei) before grape bagging (inoculation concentration was 6*10 4cfu / ml) and spray treatment (spray only once before bagging, 10 bunches of grapes per treatment). After treatment, the disease situation was investigated during the grape ripening and picking period (July 20, 2024), and the fungicidal effects of various agents were compared.

[0067] Agent 1: The bactericide is a suspension agent. The specific mass percentages are: 0.3% tetramycin, 3% picoxystrobin, 5% arylphenol polyoxyethylene succinate sulfonate, 6% lakai powder, 0.5% organosilicon, 0.1% xanthan gum, 0.1% sodium benzoate, 2% ethylene glycol, and deionized water added to 100%.

[0068] Agent 2: The bactericide is a suspension emulsion. The specific mass percentages are: 0.3% tetramycin, 3% picoxystrobin, 8% alkyl aryl polyoxyethylene ether, 6% wetting and penetrating agent F, 5.5% Tween 80, 0.4% silicone oil, 4% polyvinyl alcohol, 2% propylene glycol, and deionized water added to 100%.

[0069] Agent 3: The fungicide is a dispersible oil suspension. The specific mass percentages are: 0.3% tetramycin, 3% picoxystrobin, 6% calcium dodecylbenzenesulfonate, 4% nonylphenol polyoxyethylene ether, 6% fatty alcohol polyoxyethylene ether, 2% organic bentonite, 5% soybean oil, and 100% methyl oleate.

[0070] Experimental group 1: 2000-fold dilution of drug 1;

[0071] Experimental group 2: 2000-fold dilution of drug 1;

[0072] Experimental group 3: 2000-fold dilution of drug 1;

[0073] Control agent 1: 1000-fold dilution of tetramycin;

[0074] Control agent 2: 2000 times dilution of picoxystrobin;

[0075] Clean water control: Clean water spray.

[0076] The control effects of various fungicides on grape anthracnose are shown in Tables 7 and Figure 1 As shown:

[0077]

[0078]

[0079] The data in the above table show that the fungicide used in experimental group 2 has the highest prevention and control effect and the lowest diseased fruit rate, and has the best technical effect.

Claims

1. A composite bactericidal composition containing tetramycin and picoxystrobin, characterized in that: The composite bactericidal composition comprises tetramycin and picoxystrobin, and the mass ratio of tetramycin to picoxystrobin is 1:8-12.

2. The composite bactericidal composition containing tetramycin and picoxystrobin according to claim 1, characterized in that: The mass ratio of tetramycin to picoxystrobin is 1:

10.

3. The composite bactericidal composition containing tetramycin and picoxystrobin according to claim 1, characterized in that: The concentration of tetramycin is 0.125-4 mg / L, and the concentration of picoxystrobin is 0.003-1 mg / L.

4. The method for preparing the composite bactericidal composition containing tetramycin and picoxystrobin according to claim 1, characterized in that: Tetramycin and picoxystrobin are compounded into a suspension concentrate, emulsifiable concentrate or suspoemulsion in a mass ratio of 1:

10.

5. A bactericide, characterized in that: A composite bactericidal composition comprising any one of claims 1 to 3.

6. The fungicide according to claim 5, characterized in that The bactericide further comprises alkyl aryl polyoxyethylene ether, wetting and penetrating agent F, Tween 80, silicone oil, polyvinyl alcohol, propylene glycol and deionized water.

7. The fungicide according to claim 5, characterized in that The bactericide is a suspoemulsion.

8. Use of the composite fungicide composition according to any one of claims 1 to 3 or the fungicide according to claim 5 in the prevention and treatment of grape anthracnose.

9. The use according to claim 8, characterized in that: The composite fungicide composition according to any one of claims 1 to 7 or the fungicide according to claim 8 is sprayed on grapes.

10. The use according to claim 9, characterized in that: The spray treatment is carried out during the young fruit stage of the grapes.

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