Aqueous humor collecting device and aqueous humor collecting method

Abstract

The invention discloses an aqueous humor collecting device and method, belongs to the technical field of medical instruments, and aims to improve the aqueous humor collecting efficiency. Comprising a capillary glass blood collection tube, a first through hole sleeve, a blind hole sleeve and a puncture needle, one end of the capillary glass blood collection tube is fixedly sleeved with the first through hole sleeve, and external threads are arranged on the circumferential outer wall face of the first through hole sleeve; internal threads are formed in the inner wall surface of the blind hole sleeve and are matched with the external threads; the radius of the puncture needle is smaller than the inner radius of the capillary glass blood collection tube, one end of the puncture needle is detachably and fixedly arranged on the bottom wall of the blind hole sleeve, a through hole is formed in the bottom wall, the other part of the puncture needle is located in the capillary glass blood collection tube, and the central axis of the puncture needle coincides with the central axis of the blind hole sleeve; wherein the blind hole sleeve is screwed through the matching of the internal thread and the external thread, so that the puncture needle can extend out of the capillary glass blood collection tube.

Description

Technical Field

[0001] The present invention relates to the technical field of medical instruments, and in particular to an aqueous humor collection device and method. Background Art

[0002] In biomedical research, the collection of aqueous humor is of great significance for the study of ophthalmic diseases and drug development. Aqueous humor is the liquid between the anterior chamber and the posterior chamber of the eye, and its composition and properties can reflect the physiological and pathological conditions of the eye. At present, rats are one of the commonly used models in animal experiments, and the effective collection of aqueous humor is one of the key links in research.

[0003] The relevant method for collecting aqueous humor is as follows: first anesthetize the rat, then use tweezers to fix the eyeball of the anesthetized rat, place one end of the capillary micro-volume glass blood collection tube close to the center of the cornea of ​​the eyeball, use a 1ml injection needle to pierce a small needle hole in the center of the cornea of ​​the eyeball, and the aqueous humor is instantly sucked into the capillary micro-volume glass blood collection tube through the needle hole. Put a rubber head on the other end of the capillary glass tube, apply pressure to squeeze the aqueous humor into the ep tube to complete the collection of aqueous humor.

[0004] Although this method meets the experimental needs to a certain extent, there are many problems in the actual operation process: First, during the entire collection process, the operator needs to constantly change different tools, which makes the operation cumbersome. In addition, frequent tool changes will waste a lot of time and reduce the overall efficiency of the experiment.

[0005] The disclosure of the above background technology content is only used to assist in understanding the concept and technical solution of the present invention. It does not necessarily belong to the prior art of this patent application. In the absence of clear evidence that the above content has been disclosed on the filing date of this patent application, the above background technology should not be used to evaluate the novelty and creativity of the present application. Summary of the invention

[0006] The present application provides an aqueous humor collection device and method, which can improve the efficiency of aqueous humor collection.

[0007] To achieve the above objectives, the present application discloses the following technical solutions:

[0008] In the first aspect, an embodiment of the present application provides an aqueous humor collection device, comprising: a capillary micro-pipette glass blood sampling tube, a first through-hole sleeve, a blind-hole sleeve and a puncture needle, wherein the first through-hole sleeve is fixedly sleeved on one end of the capillary micro-pipette glass blood sampling tube, and an external thread is arranged on the circumferential outer wall surface of the first through-hole sleeve; an internal thread is provided on the inner wall surface of the blind-hole sleeve, and the internal thread and the external thread cooperate with each other; the radius of the puncture needle is smaller than the inner radius of the capillary micro-pipette glass blood sampling tube, and one end of the puncture needle is detachably fixedly provided on the bottom wall of the blind-hole sleeve, and a through hole is provided on the bottom wall, and the other part of the puncture needle is located in the capillary micro-pipette glass blood sampling tube, and the central axis of the puncture needle coincides with the central axis of the blind-hole sleeve; wherein, the cooperation of the internal thread and the external thread to tighten the blind-hole sleeve can make the puncture needle extend out of the capillary micro-pipette glass blood sampling tube.

[0009] In the embodiment of the present application, the capillary micropipette glass and the puncture needle are integrated together by the cooperation of the first through-hole sleeve and the blind hole sleeve. By tightening the blind hole sleeve, the extension and retraction of the puncture needle can be controlled without changing the tool, and a pinhole can be pierced on the cornea of ​​the rat's eyeball. In this way, the number of tool changes can be effectively reduced, thereby improving the efficiency of aqueous humor collection.

[0010] In some possible implementations of the first aspect, the aqueous humor collection device further comprises a rubber head, which is fixedly arranged on one end of the blind hole sleeve away from the capillary micro-volume glass blood collection tube and is connected to the through hole, and a vent hole is provided on the edge of the circumferential side wall of the blind hole sleeve away from the capillary micro-volume glass blood collection tube. In this way, while ensuring that both ends of the capillary micro-volume glass blood collection tube are ventilated, the rubber head is effectively integrated together, eliminating the steps of installing and removing the rubber head, thereby further improving the efficiency of aqueous humor collection.

[0011] In some possible implementations of the first aspect, the aqueous humor collection device further includes a rubber plug, an elastic ring, and a connecting rope, the rubber plug is detachably plugged into the vent hole, one end of the connecting rope is fixedly connected to the rubber plug, and the other end of the connecting rope is fixedly connected to the elastic ring, and the elastic ring is fixedly sleeved on the blind hole sleeve. In this way, when the rubber head is not needed, the rubber plug can be removed to allow air to flow through both ends of the capillary micro-volume glass blood collection tube. When the rubber head is needed, the rubber plug is inserted into the vent hole, and then the rubber head is squeezed, thereby preventing air leakage from the vent hole and improving the effect of squeezing the rubber out of the capillary micro-volume glass blood collection tube.

[0012] In some possible implementations of the first aspect, an annular avoidance groove is provided on the circumferential wall surface of the blind hole sleeve, and the elastic ring is located in the annular avoidance groove. In this way, on the one hand, the elastic ring can be fixed more securely; on the other hand, discomfort caused by the protrusion can be avoided.

[0013] In some possible implementations of the first aspect, at least one position mark is provided on the outer wall surface of the first through-hole sleeve, so as to improve the accuracy of the operation and reduce the experimental error caused by improper positioning.

[0014] In some possible implementations of the first aspect, the aqueous humor collection device further comprises a fixing block, which is detachably fixedly sleeved on one end of the capillary micro-pipette glass blood collection tube away from the first through-hole sleeve, and the fixing block is provided with a first accommodating groove and a second accommodating groove, and the aqueous humor collection device further comprises a first telescopic device, a second telescopic device, a first connecting rod, a second connecting rod, a first clamping rod, a second clamping rod, and a controller, the first telescopic device is arranged in the first accommodating groove, the second telescopic device is arranged in the second accommodating groove, the first clamping rod is fixedly connected to the telescopic rod of the first telescopic device through the first connecting rod, the second clamping rod is fixedly connected to the telescopic rod of the second telescopic device through the second connecting rod, the first clamping rod and the second clamping rod are arranged relative to each other in a first direction, the first direction is perpendicular to the central axis of the capillary micro-pipette glass blood collection tube, and the controller is electrically connected to the first telescopic device and the second telescopic device. In this way, the controller can start the first telescopic device and the second telescopic device, so that the telescopic rods of the first telescopic device and the second telescopic device are synchronously extended and retracted, and the first clamping rod and the second clamping rod are driven to move inward to clamp and fix the rat's eyeball. In this way, the clamping structures can be effectively integrated together to further improve the efficiency of aqueous humor collection.

[0015] In some possible implementations of the first aspect, the aqueous humor collection device further includes a MEMS weighing sensor, which is disposed on a surface of the first clamping rod facing the second clamping rod, and the MEMS weighing sensor is electrically connected to the controller. In this way, the controller can automatically adjust the clamping force to ensure that the applied pressure is moderate and does not cause damage to the eyeball.

[0016] In some possible implementations of the first aspect, the first receiving groove and the second receiving groove are arranged opposite to each other in the second direction, the second direction is perpendicular to the first direction, and the second direction is also perpendicular to the central axis of the capillary micro-vitro blood collection tube. In this way, the spatial layout is more reasonable, and the width of the fixing block in the first direction can be effectively reduced, thereby making the volume of the entire aqueous humor collection device more compact.

[0017] In some possible implementations of the first aspect, a limit ring is fixedly provided on the circumferential side wall of the first through-hole sleeve, so as to prevent the blind hole sleeve from moving too far and improve the safety and stability of the operation.

[0018] In a second aspect, an embodiment of the present application provides a method for collecting aqueous humor, using any of the above-mentioned aqueous humor collecting devices without a rubber plug, comprising the following steps:

[0019] 1) placing the end of the capillary micropipette glass blood collection tube away from the first through-hole sleeve close to the cornea of ​​the rat's eye;

[0020] 2) activating the first telescopic device and the second telescopic device through the controller, so that the telescopic rods of the first telescopic device and the second telescopic device are synchronously extended and retracted, thereby driving the first clamping rod and the second clamping rod to clamp and fix the eyeball of the rat;

[0021] 3) The blind hole sleeve is tightened to drive the puncture needle to move in the extension direction of the capillary micro-volume glass blood collection tube, so that the puncture needle extends out of the capillary micro-volume glass blood collection tube and a pinhole is pierced on the cornea of ​​the rat's eyeball, and the aqueous humor is instantly sucked into the capillary micro-volume glass blood collection tube through the pinhole;

[0022] 4) Release the first clamping rod and the second clamping rod from fixing the eyeball of the rat, move the end of the capillary micro-volume glass blood collection tube away from the first through-hole sleeve close to the EP tube opening, squeeze the rubber head to squeeze the aqueous humor into the EP tube, and complete the collection of aqueous humor.

[0023] In some possible implementations of the second aspect, step 3) further includes continuing to activate the first telescopic device and the second telescopic device so that the first clamping rod and the second clamping rod apply pressure to the eyeball to make more aqueous humor flow out of the pinhole.

[0024] Since the aqueous humor collection method provided in the embodiment of the present application includes the aqueous humor collection device described in the above technical solution without a rubber plug, the two can solve the same technical problem and achieve the same technical effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following briefly introduces the drawings required for the embodiments or the prior art descriptions. It should be understood that in all the drawings, the same reference numerals represent the same elements. In the drawings, for the sake of clarity and ease of understanding, the sizes of some features may be deformed.

[0026] Figure 1 A schematic diagram of the structure of an aqueous humor collection device provided in some embodiments of the present application;

[0027] Figure 2 For only Figure 1 A schematic structural diagram of a cross-section view of a first through-hole sleeve in the aqueous humor collection device shown;

[0028] Figure 3 for Figure 2 An enlarged view of the circled portion of the structure shown at A;

[0029] Figure 4 for Figure 1 A schematic diagram of the internal structure of the partial structure of the aqueous humor collection device shown;

[0030] Figure 5 for Figure 4 Exploded view of the structure shown.

[0031] Description of reference numerals:

[0032] 10. Capillary micro-pipette glass blood collection tube; 20. First through-hole sleeve; 201. Limiting ring; 30. Blind hole sleeve; 301. Through hole; 302. Air vent; 303. Annular avoidance groove; 40. Puncture needle; 50. Rubber head; 60. Rubber plug; 601. Elastic ring; 602. Connecting rope; 70. Fixed block; 701. First accommodating groove; 702. Second accommodating groove; 80. First telescopic device; 90. Second telescopic device; 100. First connecting rod; 110. Second connecting rod; 120. First clamping rod; 130. Second clamping rod; 140. Controller. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] It should be noted that, in the description of the present invention, terms such as "center", "upper", "lower", "horizontal", "inner", etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0035] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0036] See also Figure 1 Combined with Figure 2 and Figure 3The embodiment of the present application provides an aqueous humor collection device, comprising: a capillary micro-pipette glass sampling tube 10, a first through-hole sleeve 20, a blind hole sleeve 30 and a puncture needle 40, wherein the first through-hole sleeve 20 is fixedly sleeved on one end of the capillary micro-pipette glass sampling tube 10. Exemplarily, the first through-hole sleeve 20 and the capillary micro-pipette glass sampling tube 10 can be fixedly connected by, but not limited to, bonding. An external thread is provided on the circumferential outer wall surface of the first through-hole sleeve 20; an internal thread is provided on the inner wall surface of the blind hole sleeve 30, and the internal thread and the external thread cooperate with each other; the radius of the puncture needle 40 is smaller than the inner radius of the capillary micro-pipette glass sampling tube 10, and one end of the puncture needle 40 is detachably fixedly provided on the bottom wall of the blind hole sleeve 30. Exemplarily, a rubber block is fixedly provided on the bottom wall of the blind hole sleeve 30, and a detachable fixed connection between the puncture needle 40 and the blind hole sleeve 30 is achieved by inserting one end of the puncture needle 40 into the rubber block. Specifically, the rubber block can be fixedly connected to the bottom wall of the blind hole sleeve 30 by bonding, screw connection, etc. Specifically, the rubber block can be directly fixedly connected to the bottom wall of the blind hole sleeve 30, or can be indirectly fixedly connected to the bottom wall of the blind hole sleeve 30 by a connecting plate or other structure. It can be understood that the subsequent fixed connection method between various components can be designed with reference to the connection method between the rubber block and the bottom wall of the blind hole sleeve 30, and no further description is given.

[0037] Of course, the present application is not limited thereto. In other embodiments, a magnetic block is fixedly disposed on the bottom wall of the blind hole sleeve 30, and a detachable fixed connection between the puncture needle 40 and the blind hole sleeve 30 is achieved by inserting one end of the puncture needle 40 into the plug hole provided on the magnetic block.

[0038] A through hole 301 is provided on the bottom wall, and the other part of the puncture needle 40 is located in the capillary micro-volume glass blood collection tube 10, and the central axis of the puncture needle 40 coincides with the central axis of the blind hole sleeve 30; wherein, the inner thread and the outer thread cooperate to tighten the blind hole sleeve 30, so that the puncture needle 40 can extend out of the capillary micro-volume glass blood collection tube 10.

[0039] The capillary micropipette glass and the puncture needle 40 are integrated together by the cooperation of the first through-hole sleeve 20 and the blind hole sleeve 30. By tightening the blind hole sleeve 30, the extension and retraction of the puncture needle 40 can be controlled without changing the tool, and a pinhole can be pierced on the cornea of ​​the rat's eyeball. In this way, the number of tool changes can be effectively reduced, thereby improving the efficiency of aqueous humor collection.

[0040] See also Figure 2In some embodiments, the aqueous humor collection device further includes a rubber head 50, which is fixedly arranged on one end of the blind hole sleeve 30 away from the capillary micro-volume glass sampling tube 10 and communicated with the through hole 301. A vent hole 302 is provided on the edge of the circumferential side wall of the blind hole sleeve 30 away from the capillary micro-volume glass sampling tube 10. In this way, while ensuring that both ends of the capillary micro-volume glass sampling tube 10 are ventilated, the rubber head 50 is effectively integrated together, eliminating the steps of installing and disassembling the rubber head 50, thereby further improving the efficiency of aqueous humor collection.

[0041] See also Figure 2 and Figure 3 In some embodiments, the aqueous humor collection device further includes a rubber plug 60, an elastic ring 601 and a connecting rope 602. The rubber plug 60 is detachably inserted into the vent hole 302. One end of the connecting rope 602 is fixedly connected to the rubber plug 60, and the other end of the connecting rope 602 is fixedly connected to the elastic ring 601. The elastic ring 601 is fixedly sleeved on the blind hole sleeve 30. In this way, when the rubber head 50 is not needed, the rubber plug 60 can be removed to allow air to pass through both ends of the capillary micro-amount glass blood collection tube 10. When the rubber head 50 is needed, the rubber plug 60 is inserted into the vent hole 302, and then the rubber head 50 is squeezed, thereby preventing the vent hole 302 from leaking air and improving the effect of squeezing the rubber out of the capillary micro-amount glass blood collection tube 10.

[0042] See also Figure 3 In some embodiments, an annular avoidance groove 303 is provided on the circumferential wall of the blind hole sleeve 30, and the elastic ring 601 is located in the annular avoidance groove 303. In this way, on the one hand, the elastic ring 601 can be fixed more firmly; on the other hand, the discomfort caused by the protrusion can be avoided.

[0043] See also Figure 2 In some embodiments, a limit ring 201 is fixedly disposed on the circumferential side wall of the first through hole sleeve 20. In this way, the blind hole sleeve 30 can be prevented from moving too far, thereby improving the safety and stability of the operation.

[0044] In some embodiments, at least one position mark is disposed on the outer wall surface of the first through-hole sleeve 20. In this way, the accuracy of the operation can be improved and the experimental error caused by improper positioning can be reduced.

[0045] See also Figure 4 Combined with Figure 5In some embodiments, the aqueous humor collection device further includes a fixing block 70, which is detachably fixedly sleeved on one end of the capillary micropipette glass blood collection tube 10 away from the first through-hole sleeve 20. The fixing block 70 is provided with a first receiving groove 701 and a second receiving groove 702. The aqueous humor collection device further includes a first telescopic device 80, a second telescopic device 90, a first connecting rod 100, a second connecting rod 110, a first clamping rod 120, a second clamping rod 130, and a controller 140. The first telescopic device 80 is arranged in the first receiving groove 701, and the second telescopic device 90 is arranged in the second receiving groove 702. The first clamping rod 120 is fixedly connected to the telescopic rod of the first telescopic device 80 through the first connecting rod 100, and the second clamping rod 130 is fixedly connected to the telescopic rod of the second telescopic device 90 through the second connecting rod 110. Exemplarily, the first telescopic device 80 and the second telescopic device 90 may be micro electric push rods.

[0046] The first clamping rod 120 and the second clamping rod 130 are arranged relative to each other in a first direction, and the first direction is perpendicular to the central axis of the capillary micro-pipette glass blood collection tube 10. The controller 140 is electrically connected to the first telescopic device 80 and the second telescopic device 90. Exemplarily, the controller 140 can be a microcomputer terminal or a single-chip microcomputer. The controller 140 has three push switches for controlling the first telescopic device 80 and the second telescopic device 90 of the controller 140 to extend, pause, and shorten. Of course, the present application is not limited to this. In other embodiments, the controller 140 can also push a switch, and the push switch has three push positions for controlling the first telescopic device 80 and the second telescopic device 90 to extend, pause, and shorten. Another exemplary embodiment, the controller 140 can be set on the outer wall surface of the fixed block 70.

[0047] In this way, the controller 140 can start the first telescopic device 80 and the second telescopic device 90, so that the telescopic rods of the first telescopic device 80 and the second telescopic device 90 are synchronously extended and retracted, driving the first clamping rod 120 and the second clamping rod 130 to move inward to clamp and fix the rat's eyeball. In this way, the clamping structure can be effectively integrated together to further improve the efficiency of aqueous humor collection.

[0048] In some embodiments, the detachable fixed connection between the fixing block 70 and the capillary micro-pipette glass sampling tube 10 can be designed with reference to the detachable fixed connection between the blind hole sleeve 30 and the capillary micro-pipette glass sampling tube 10, that is, a second through-hole sleeve is sleeved on the end of the capillary micro-pipette glass sampling tube 10 away from the first through-hole sleeve 20, and an external thread is arranged on the outer wall of the second through-hole sleeve; a threaded hole matching the external thread on the outer wall of the second through-hole sleeve is arranged on the fixing block 70. It can be understood that the fixing block 70 can be disassembled and installed by loosening or tightening the fixing block 70.

[0049] On the basis of the above embodiment, the end of the capillary micro-volume glass blood sampling tube 10 away from the first through-hole sleeve 20 is tapered. In this way, when the puncture needle 40 is operated to pierce a needle hole on the cornea of ​​the rat's eyeball, the capillary micro-volume glass blood sampling tube 10 can be rotated with the other hand by holding the fixing block 70 with one hand, and the end of the capillary micro-volume glass blood sampling tube 10 away from the first through-hole sleeve 20 can also pass through the cornea of ​​the rat's eyeball by using the tapered shape, thereby being more conducive to the collection of aqueous humor.

[0050] Of course, the present application is not limited thereto. In other embodiments, the end of the capillary micro-pipette glass blood sampling tube 10 away from the first through-hole sleeve 20 may also be round. It is understandable that the specific shape of the end of the capillary micro-pipette glass blood sampling tube 10 away from the first through-hole sleeve 20 is determined according to actual needs.

[0051] In some embodiments, the aqueous humor collection device further includes a MEMS weighing sensor, which is disposed on the surface of the first clamping rod 120 facing the second clamping rod 130, and the MEMS weighing sensor is electrically connected to the controller 140. In this way, the controller 140 can automatically adjust the clamping force to ensure that the applied pressure is moderate and does not cause damage to the eyeball.

[0052] See also Figure 4 Combined with Figure 5 In some embodiments, the first receiving groove 701 and the second receiving groove 702 are arranged opposite to each other in the second direction, the second direction is perpendicular to the first direction, and the second direction is also perpendicular to the central axis of the capillary micro-pipette glass blood sampling tube 10. In this way, the spatial layout is more reasonable, and the width of the fixing block 70 in the first direction can be effectively reduced, so that the volume of the entire aqueous humor collection device is more compact.

[0053] The embodiment of the present application also provides a method for collecting aqueous humor, which uses any of the above-mentioned aqueous humor collecting devices without the rubber plug 60 and includes the following steps:

[0054] 1) placing the end of the capillary micropipette glass blood collection tube 10 away from the first through-hole sleeve 20 close to the cornea of ​​the rat's eye;

[0055] Specifically, the capillary micro-pipette glass blood collection tube 10 can be placed away from the end of the first through-hole sleeve 20 close to the center of the rat's cornea. Of course, the present application is not limited to this. In other embodiments, the capillary micro-pipette glass blood collection tube 10 can be placed away from the end of the first through-hole sleeve 20 close to other positions of the rat's cornea according to actual needs. It is understandable that the rat eyeball can be a rat eyeball that has been removed or a rat eyeball that has not been removed (in this case, the rat needs to be anesthetized).

[0056] 2) activating the first telescopic device 80 and the second telescopic device 90 through the controller 140, so that the telescopic rods of the first telescopic device 80 and the second telescopic device 90 are synchronously extended and retracted, thereby driving the first clamping rod 120 and the second clamping rod 130 to clamp and fix the eyeball of the rat;

[0057] 3) By tightening the blind hole sleeve 30, the puncture needle 40 is driven to move in the extension direction of the capillary micro-volume glass blood sampling tube 10, so that the puncture needle 40 extends out of the capillary micro-volume glass blood sampling tube 10, and a pinhole is pierced on the cornea of ​​the rat's eye. Through the pinhole, the aqueous humor is instantly sucked into the capillary micro-volume glass blood sampling tube 10; it can be understood that the position of the pinhole on the cornea of ​​the rat's eye is determined according to actual needs and is not limited here. For example, a pinhole can be pierced in the center of the rat's cornea.

[0058] 4) Release the first clamping rod 120 and the second clamping rod 130 from fixing the eyeball of the rat, move the capillary micro-volume glass blood collection tube 10 away from the end of the first through-hole sleeve 20 close to the EP tube opening, squeeze the rubber head 50 to squeeze the aqueous humor into the EP tube, and complete the collection of aqueous humor.

[0059] Preferably, step 3) further comprises continuing to activate the first telescopic device 80 and the second telescopic device 90 so that the first clamping rod 120 and the second clamping rod 130 apply pressure to the eyeball, so that more aqueous humor flows out of the pinhole toward the cornea.

[0060] It is understandable that when using an aqueous humor collection device with a rubber plug 60, before step 3), the rubber plug 60 needs to be removed to allow air to flow through both ends of the capillary micro-volume glass blood collection tube 10, and before squeezing the rubber tip 50, the rubber plug 60 needs to be inserted into the vent hole 302.

[0061] The present invention and its embodiments are described above, and such description is not restrictive. What is shown in the full text is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A device for collecting aqueous humor, characterized in that: include: Capillary micro-volume glass blood collection tube; A first through-hole sleeve, wherein the first through-hole sleeve is fixedly sleeved on one end of the capillary micro-vitro glass blood collection tube, and an external thread is arranged on the circumferential outer wall surface of the first through-hole sleeve; A blind hole sleeve, wherein an internal thread is provided on the inner wall surface of the blind hole sleeve, and the internal thread cooperates with the external thread; A puncture needle, the radius of which is smaller than the inner radius of the capillary micro-volume glass blood sampling tube, one end of which is detachably fixed on the bottom wall of the blind hole sleeve, a through hole being provided on the bottom wall, the other part of which is located in the capillary micro-volume glass blood sampling tube, and the central axis of the puncture needle coincides with the central axis of the blind hole sleeve; wherein, by tightening the blind hole sleeve through the cooperation of the internal thread and the external thread, the puncture needle can be extended out of the capillary micro-volume glass blood sampling tube.

2. The aqueous humor collection device according to claim 1, characterized in that: It also includes a rubber head, which is fixedly arranged on one end of the blind hole sleeve away from the capillary micro-amount glass blood sampling tube and is connected to the through hole. A vent hole is opened on the edge of one side of the circumferential side wall of the blind hole sleeve away from the capillary micro-amount glass blood sampling tube.

3. The aqueous humor collection device according to claim 2, characterized in that: It also includes a rubber plug, an elastic ring and a connecting rope. The rubber plug is detachably inserted into the vent hole. One end of the connecting rope is fixedly connected to the rubber plug, and the other end of the connecting rope is fixedly connected to the elastic ring. The elastic ring is fixedly sleeved on the blind hole sleeve.

4. The aqueous humor collection device according to claim 3, characterized in that: An annular avoidance groove is provided on the circumferential wall surface of the blind hole sleeve, and the elastic ring is located in the annular avoidance groove.

5. The aqueous humor collection device according to claim 4, characterized in that: At least one position mark is arranged on the outer wall surface of the first through hole sleeve.

6. The aqueous humor collection device according to claim 3, characterized in that: The apparatus further comprises a fixing block, which is detachably fixedly mounted on one end of the capillary micro-volume glass blood sampling tube away from the first through-hole sleeve, and the fixing block is provided with a first accommodating groove and a second accommodating groove. The aqueous humor collection device further comprises a first telescopic device, a second telescopic device, a first connecting rod, a second connecting rod, a first clamping rod, a second clamping rod, and a controller. The first telescopic device is arranged in the first accommodating groove, and the second telescopic device is arranged in the second accommodating groove. The first clamping rod is fixedly connected to the telescopic rod of the first telescopic device through the first connecting rod, and the second clamping rod is fixedly connected to the telescopic rod of the second telescopic device through the second connecting rod. The first clamping rod and the second clamping rod are arranged opposite to each other in a first direction, and the first direction is perpendicular to the central axis of the capillary micro-volume glass blood sampling tube. The controller is electrically connected to the first telescopic device and the second telescopic device.

7. The aqueous humor sampling device according to claim 6, characterized in that: The first accommodating groove and the second accommodating groove are arranged opposite to each other in a second direction, the second direction is perpendicular to the first direction, and the second direction is also perpendicular to the central axis of the capillary micro-particle glass blood collection tube.

8. The aqueous humor sampling device according to claim 6 or 7, characterized in that: A limiting ring is fixedly arranged on the circumferential side wall of the first through hole sleeve.

9. A method for collecting aqueous humor, characterized in that: The aqueous humor collection device according to claim 6 comprises the following steps: 1) placing the end of the capillary micropipette glass blood collection tube away from the first through-hole sleeve close to the cornea of ​​the rat's eye; 2) activating the first telescopic device and the second telescopic device through a controller, so that the telescopic rods of the first telescopic device and the second telescopic device are synchronously extended and retracted, thereby driving the first clamping rod and the second clamping rod to clamp and fix the eyeball of the rat; 3) tightening the blind hole sleeve to drive the puncture needle to move in the extension direction of the capillary micro-volume glass blood sampling tube, so that the puncture needle extends out of the capillary micro-volume glass blood sampling tube and pokes a pinhole on the cornea of ​​the rat's eyeball, and the aqueous humor is instantly sucked into the capillary micro-volume glass blood sampling tube through the pinhole; 4) Release the first clamping rod and the second clamping rod from fixing the eyeball of the rat, move the capillary micro-volume glass blood collection tube away from the first through-hole sleeve and close to the EP tube opening, squeeze the rubber head to squeeze the aqueous humor into the EP tube, and complete the collection of aqueous humor.

10. The aqueous humor collection method according to claim 9, characterized in that: Step 3) also includes continuing to activate the first telescopic device and the second telescopic device so that the first clamping rod and the second clamping rod apply pressure to the eyeball, causing more aqueous humor to flow out of the pinhole.

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