Night oral care solution for improving oral environment

Abstract

According to the night oral care solution for improving the oral environment provided by the invention, the probiotics, the antioxidant and the antibacterial agent jointly form a synergistic system to ensure the health and balance of the oral environment at night, and meanwhile, the oral care solution can be used for improving the oral environment at night. The low-water activity protective agent and the surfactant are added to be matched with the probiotics, so that the probiotics can keep activity before being used and are uniformly dispersed in the oral care solution.

Description

Technical Field

[0001] The invention relates to the field of oral care, in particular to a night-time oral care liquid for improving the environment in the oral cavity. Background Art

[0002] Oral care solution, commonly known as mouthwash, is a liquid preparation used to maintain oral hygiene. It can not only clean the mouth and remove food residues, but also inhibit or kill bacteria in the mouth and prevent the occurrence of oral diseases such as caries and periodontal disease. In addition, some specially formulated mouthwashes also have anti-inflammatory, analgesic, and oral mucosal repair effects, which play an important role in maintaining oral health.

[0003] At night, the human body enters a resting state, oral activity decreases, and saliva secretion decreases accordingly. Saliva is a natural cleanser for the oral cavity. Its reduction will lead to a decrease in the oral self-cleaning ability, providing a suitable environment for the reproduction of bacteria. Studies have shown that there are many types of bacteria in the mouth, and their number is huge. They multiply rapidly under suitable conditions, which may cause problems such as bad breath, tooth decay and even periodontal disease. Especially when you do not eat for a long time at night, food residues in the mouth become a breeding ground for bacteria, further aggravating the imbalance of the oral microecology.

[0004] In addition, the dry oral environment at night also provides advantages for some aerobic bacteria, which consume oxygen in the mouth and produce metabolites, such as volatile sulfides, which are the main components of bad breath. Therefore, maintaining the balance of oral flora at night is essential for preventing oral diseases and keeping breath fresh.

[0005] The development of night-time oral care solution is precisely to meet the special needs of the oral environment at night. It not only needs to have a good antibacterial effect to reduce the number of bacteria and maintain oral hygiene, but also needs to be able to promote saliva secretion and improve oral dryness. Through continuous protection at night, oral care solution helps maintain oral health and lays the foundation for fresh breath and good oral condition the next day. In the prior art, by adding probiotics, such as Streptococcus salivarius and Lactobacillus rhamnosus, the growth of harmful bacteria can be effectively inhibited, and through competitive exclusion, production of antibacterial substances, and enhancement of the barrier function of the oral mucosa, it has a positive effect on maintaining oral health. They help to balance the microbial community in the oral cavity, improve the oral cavity's resistance to disease, while reducing oral inflammation and reducing the risk of gingival bleeding.

[0006] However, the current formulation and use of night-time oral care solutions do not fully meet the complex needs of the nighttime oral environment. In particular, the addition of probiotics, although in theory it can provide beneficial bacteria to the oral cavity and improve the balance of the microecology, in actual applications, the stability and activity of probiotics remain a technical challenge. The survival rate of probiotics in oral care solutions, the maintenance of their activity during storage and use, and their effective release and action in the oral environment are all key points that require further research and improvement. Summary of the invention

[0007] The present invention aims to provide a night-time oral care solution that improves the oral environment. By using the oral care solution before going to bed, the oral cavity can continue to be antibacterial, maintain the beneficial bacteria in the oral cavity, inhibit the consumption of oxygen in the oral cavity by aerobic bacteria, and keep the breath fresh at night. The oral care solution provided by the present invention is added with probiotics, and the activity of the probiotics is maintained and effectively released during the storage and use of the oral care solution through the selection of specific components. In addition, the present invention also provides a preparation method of the oral care solution and its application in oral care. The details are as follows:

[0008] A night-time oral care solution for improving the intraoral environment, comprising the following components and contents based on the total mass of the oral care solution:

[0009] Probiotics: 0.01wt%-0.1wt%;

[0010] Antimicrobial agent: 0.05wt%-0.2wt%;

[0011] Antioxidant: 0.01wt%-0.5wt%;

[0012] Low water activity protective agent: 0.1wt%-0.5wt%;

[0013] pH regulator: 0.5wt%-2wt%;

[0014] Surfactant 0.05wt%-0.5wt%;

[0015] and the balance of water;

[0016] The probiotics are selected from one or more of Streptococcus salivarius K12 or Lactobacillus rhamnosus GG; the number of live bacteria of the probiotics in the oral care solution is maintained at 5×10 within 180 days from the date of preparation. 7 Up to 10 11 CFU / mL, the survival rate of live bacteria is above 80%.

[0017] In one embodiment, the low water activity protective agent is camellia seed oil; the surfactant is a nonionic surfactant and a zwitterionic surfactant, including one or more of soybean lecithin, polysorbate, cocamidopropyl betaine, alkyl glucoside, decyl glucoside, and polyoxyethylene (20) castor oil, among which polysorbate is preferred.

[0018] In one embodiment, the antibacterial agent is selected from one or more of domiphene, chlorhexidine, phenoxyethanol, and parabens.

[0019] In one embodiment, the antioxidant is selected from one or more of vitamin C (ascorbic acid), vitamin E (tocopherol), lipoic acid, phytic acid, caffeic acid, gallic acid, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT).

[0020] In one of the solutions, based on the total mass of the oral care solution, the following components and contents are also included:

[0021] Fragrance modifier 0wt%-3wt%; preferably 1wt%-2wt%;

[0022] Sweetener 0wt%-1wt%; preferably 0.2wt%-0.6wt%.

[0023] In one approach, the probiotics are protected against low water activity by:

[0024] (1) mixing probiotics with a low water activity protective agent and a surfactant;

[0025] (2) Perform ultrasonic homogenization at a low power of 100-200w for 10-30 seconds;

[0026] Preferably, the temperature is controlled within the range of 0-25°C and the humidity is controlled within the range of 45-65% during the low water activity protection step.

[0027] In one of the protocols, the pH of the nighttime oral care solution was between 6.5 and 7.5.

[0028] In one embodiment, the pH adjuster is selected from one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium bicarbonate and sodium carbonate, preferably sodium bicarbonate or disodium hydrogen phosphate.

[0029] In one embodiment, the flavor modulator is selected from one or more of pineapple extract, menthol, limonene, and carvone.

[0030] In one embodiment, the sweetener is selected from one or more of sucralose, aspartame, stevia or erythritol.

[0031] The present invention also provides a method for preparing a night-time oral care solution for improving the oral environment, comprising the following steps:

[0032] (1) Preparation of probiotics: culturing and activating the probiotic strains, collecting them by centrifugation or filtration, and washing to remove impurities in the culture medium;

[0033] (2) Low water activity protection of probiotics: mixing probiotics with a low water activity protective agent and a surfactant; performing ultrasonic homogenization at a low power of 100-200w for 10-30 seconds to obtain homogenized probiotics with low water activity protection;

[0034] (3) Oral care solution mixing: dissolving the antibacterial agent and antioxidant in water according to the ratio, and then adding the homogenized low water activity protected probiotics, and adding the pH adjuster to adjust the pH value of the final product to between 6.5 and 7.5, to form a night-time oral care solution for improving the oral environment.

[0035] In one embodiment, in step (2), the temperature is controlled within the range of 0-25°C and the humidity is controlled within the range of 45-65%;

[0036] In one embodiment, in step (3), the method further includes adding one or more of a flavor regulator and a sweetener at the same time as the antibacterial agent and the antioxidant according to a ratio.

[0037] The invention also provides an application of the oral care liquid in oral care at night.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) Enhanced antibacterial effect at night: Probiotics, antioxidants and antibacterial agents together form a synergistic system to ensure a healthy and balanced oral environment at night. Probiotics such as Streptococcus salivarius K12 and Lactobacillus rhamnosus GG occupy the attachment points of the oral mucosa, competitively exclude harmful bacteria, and enhance the barrier function of the oral mucosa, thereby maintaining the natural balance of the oral flora. Antioxidants, such as vitamin C or vitamin E, not only protect probiotics from damage caused by oxidative stress and maintain their activity, but also neutralize free radicals that may damage oral cells, providing a stable environment for probiotics so that they can continue to work at night. Antibacterial agents, which are non-broad-spectrum antibacterial agents with less impact on probiotics, are selected to directly kill or inhibit harmful bacteria in the mouth, and work together with probiotics to maintain oral hygiene, while reducing the risk of probiotics being subjected to external environmental pressure. By maintaining the balance of oral flora and reducing the problem of bacterial reproduction aggravated by reduced saliva secretion, it helps prevent the occurrence of caries and periodontal disease, while reducing oral odor caused by bacterial activity at night.

[0040] (2) Long-term maintenance of probiotic activity: At the same time, low water activity protective agents and surfactants are combined with probiotics to enable the probiotics to maintain their activity and uniform dispersion in the oral care solution before use. It is specifically speculated that low water activity protective agents, such as camellia oil, slow down their metabolic activity by reducing the water activity around the probiotics, thereby extending the dormancy period of the probiotics and maintaining their activity. At the same time, surfactants, such as polysorbate, use their hydrophilic and lipophilic groups to reduce the oil-water interfacial tension, promote the uniform dispersion of probiotics in the oral care solution, prevent aggregation and precipitation, and improve stability. The use of low water activity protective agents and surfactants in combination with probiotics not only protects the probiotics from adverse environmental factors during storage, but also ensures the rapid release and effective action of the probiotics when used in the oral cavity, thereby providing continuous protection and freshness to the oral cavity throughout the night. The dispersibility of the probiotics is further enhanced by ultrasonic homogenization. DETAILED DESCRIPTION

[0041] Although the present invention has been described to a certain extent, it is obvious that appropriate changes in various conditions can be made without departing from the spirit and scope of the present invention. It is understood that the present invention is not limited to the embodiments described, but belongs to the scope of the claims, which includes equivalent replacements of each factor described.

[0042] In the oral care solution of the present invention, the main function of probiotics is to help improve the microbial balance in the oral cavity. They can play a role through a variety of mechanisms, including competitive inhibition of the adhesion and colonization of harmful bacteria, neutralization of virus-causing toxins, and possibly maintaining the balance of colonies in the oral cavity by regulating the body's immune response. These effects help prevent oral diseases such as caries and periodontal disease. In particular, the two probiotics, Streptococcus salivarius K12 and Lactobacillus rhamnosus GG, can resist cariogenic bacteria and reduce the number of Streptococcus mutans in saliva, thereby reducing the incidence of caries.

[0043] The probiotic content is controlled within the range of 0.01wt%-0.1wt% to ensure that the product maintains effectiveness while also ensuring safety and stability. This content range helps the probiotics to work in the oral cavity while avoiding possible side effects or incompatibility with other ingredients caused by excessive concentrations. The probiotic content is preferably 0.02wt%-0.08wt% based on the total mass of the oral care solution, and more preferably 0.04wt%-0.06wt%. The number of live bacteria is guaranteed to remain at 5×10 within 180 days from the date of preparation. 7 Up to 10 11CFU / mL is to ensure that the product can provide enough live bacteria throughout the shelf life to improve the oral environment. Based on the oral care liquid composition of the present invention, the viable survival rate of probiotics within 180 days is more than 80%, preferably more than 85%.

[0044] In the oral care solution of the present invention, broad-spectrum antibacterial agents such as triclosan and triclocarban are avoided when selecting antibacterial agents, mainly because although these broad-spectrum antibacterial agents have a wide range of antibacterial effects, they may have an adverse effect on probiotics, thereby destroying the natural balance of microorganisms in the oral cavity. The oral microbiome is an important barrier against foreign pathogens, and broad-spectrum antibacterial agents may not only inhibit harmful bacteria, but also inhibit beneficial bacteria, leading to an imbalance in the oral microbiome.

[0045] The antibacterial agents such as domiphene, chlorhexidine, phenoxyethanol, and parabens are selected because they are relatively more targeted and can reduce the impact on probiotics while inhibiting harmful bacteria. For example, domiphene is a surfactant with a bactericidal effect, but relative to broad-spectrum antibacterial agents, it may have less impact on probiotics. Chlorhexidine is a commonly used antibacterial agent with good antibacterial effect, but the impact on some probiotics may be small. Phenoxyethanol and parabens are also commonly used preservatives, and they usually have limited impact on probiotics at low concentrations. Therefore, the content of the antibacterial agent is controlled at 0.05wt%-0.2wt%; preferably 0.07wt%-0.18wt%; more preferably 0.1wt%-0.15wt%.

[0046] The oral care solution of the present invention further comprises an antioxidant, which is selected from one or more of vitamin C (ascorbic acid), vitamin E (tocopherol), lipoic acid, phytic acid, caffeic acid, gallic acid, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT), preferably vitamin C (ascorbic acid) and vitamin E (tocopherol). Antioxidants can effectively remove free radicals in the oral cavity and reduce oxidative stress, which is particularly important for preventing and treating oral diseases such as periodontitis.

[0047] The oral care solution described in the present invention also contains surfactants. Surfactants mainly play emulsifying, wetting, dispersing and solubilizing roles in the oral care solution. They can stabilize the various ingredients in the product formula, improve the solubility and bioavailability of the active ingredients, enhance the cleaning effect of the product, improve the taste, and at the same time help the adsorption and distribution of probiotics on the oral mucosa, thereby improving the overall effect and user experience of the oral care solution.

[0048] It can be seen that the first layer of the present invention, as the three essential components at the core of the present invention, probiotics, antioxidants and antibacterial agents together constitute a synergistic system to ensure the health and balance of the oral environment at night. Probiotics such as Streptococcus salivarius K12 and Lactobacillus rhamnosus GG, by occupying the attachment points of the oral mucosa, competitively exclude harmful bacteria, enhance the barrier function of the oral mucosa, and thus maintain the natural balance of the oral flora. Antioxidants, such as vitamin C or vitamin E, not only protect probiotics from damage caused by oxidative stress and maintain their activity, but also neutralize free radicals that may cause damage to oral cells, providing a stable environment for probiotics so that they can continue to play a role at night. Antibacterial agents, non-broad-spectrum antibacterial agents with less effect on probiotics are selected, and by directly killing or inhibiting harmful bacteria in the mouth, they work together with probiotics to maintain oral hygiene, while reducing the risk of probiotics being subjected to external environmental pressure.

[0049] The oral care liquid of the present invention also includes a low water activity protective agent, which is an additive used to reduce water activity (Aw) in food, medicine and biological products, and its purpose is to reduce the free water required for microbial growth, thereby extending the shelf life of the product and maintaining its stability. In mouthwash, the role of the low water activity protective agent is to reduce microbial growth, while maintaining the stability of probiotics and other ingredients, ensuring the effectiveness of the product during the shelf life. In oral care liquid products containing probiotics, it is necessary to ensure the activity of probiotics, so the addition amount of the low water activity protective agent should be able to effectively reduce the water activity, and not inhibit the probiotics. Experiments show that when camellia seed oil is selected and the content is controlled at 0.1wt%-0.5wt%, the water activity can be effectively reduced, and the probiotics will not be inhibited. When using polyols such as sorbitol, glycerol, propylene glycol, etc. commonly used in the art, it is necessary to reduce the water activity and the antiseptic effect at a higher concentration (above 1wt%), but there are also inhibitory side effects on probiotics.

[0050] The present invention selects nonionic surfactants and zwitterionic surfactants, including soybean lecithin, polysorbate, cocamidopropyl betaine, alkyl glycosides, decyl glucoside, polyoxyethylene (20) castor oil or more. Compared with anionic surfactants, nonionic surfactants and zwitterionic surfactants are milder, less irritating to oral mucosa and probiotics, and have good biocompatibility, can coexist with probiotics, and will not have a negative impact on the metabolic activity of probiotics, preferably polysorbate, which can further remain stable and not ionized in a wide pH range. The content of the surfactant is 0.05wt%-0.5wt%, preferably 0.1wt%-0.4wt%, and more preferably 0.2wt%-0.35wt%.

[0051] The probiotics are protected from low water activity by:

[0052] (1) mixing probiotics with a low water activity protective agent and a surfactant;

[0053] (2) Perform ultrasonic homogenization at a low power of 100-200w for 10-30 seconds;

[0054] Preferably, the temperature is controlled at 0-25°C and the humidity is controlled at 45-65% in the low water activity protection step. Low power helps to reduce thermal effects, and a short treatment time can prevent long-term mechanical shearing forces from damaging probiotic cells. In addition, under preferred conditions, the temperature is controlled at 0-25°C and the humidity is controlled at 45-65%, which helps to maintain the activity of probiotics and reduce the adverse effects of environmental factors on their stability. Ultrasonic homogenization further enhances the dispersibility of probiotics while maximizing the activity of probiotics.

[0055] It can be seen that the second layer of the present invention is a low water activity protective agent and a surfactant in combination with probiotics to enable the probiotics to maintain activity and uniform dispersion in the oral care solution before use. It is specifically speculated that a low water activity protective agent, such as camellia oil, slows down its metabolic activity by reducing the water activity around the probiotics, thereby extending the dormancy period of the probiotics and maintaining their activity. At the same time, surfactants, such as polysorbates, utilize their hydrophilic and lipophilic groups to reduce the oil-water interfacial tension, promote the uniform dispersion of probiotics in the oral care solution, prevent aggregation and precipitation, and improve stability. The use of low water activity protective agents and surfactants in combination with probiotics not only protects the probiotics from adverse environmental factors during storage, but also ensures the rapid release and effective action of the probiotics when used in the oral cavity, thereby providing continuous protection and freshness for the oral cavity throughout the night.

[0056] The oral care solution of the present invention further comprises a pH regulator, which is selected from one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium bicarbonate and sodium carbonate, preferably sodium bicarbonate or disodium hydrogen phosphate. The pH value of the oral care solution is adjusted by the pH regulator, and the pH value of the oral care solution is between 6.5 and 7.5, providing an environment close to the natural pH value of the oral cavity (usually between 6.5 and 7.5), reducing irritation to the oral mucosa, and helping to maintain the activity of probiotics in the oral cavity, preferably sodium bicarbonate or disodium hydrogen phosphate as the pH regulator.

[0057] The oral care solution of the present invention may further contain a flavor regulator and a sweetener. The flavor regulator is selected from one or more of pineapple extract, menthol, limonene, and carvone. The sweetener is selected from one or more of sucralose, aspartame, stevioside, or erythritol. The oral care solution may further provide a pleasant aroma and taste, and cover up possible unpleasant tastes.

[0058] The present invention also provides a method for preparing a night-time oral care solution for improving the oral environment, comprising the following steps:

[0059] (1) Preparation of probiotics: culturing and activating the probiotic strains, collecting them by centrifugation or filtration, and washing to remove impurities in the culture medium;

[0060] (2) Low water activity protection of probiotics: the probiotics are mixed with a low water activity protective agent and a surfactant; ultrasonic homogenization is performed at a low power of 100-200w for 10-30 seconds to obtain homogenized low water activity protected probiotics; further, the temperature is controlled at 0-25°C and the humidity is controlled in the range of 45-65%;

[0061] (3) Oral care solution mixing: The antibacterial agent and antioxidant are dissolved in water according to the ratio, and then the homogenized low water activity protected probiotics are added, and a pH adjuster is added to adjust the pH value of the final product to between 6.5 and 7.5 to form a night-time oral care solution that improves the oral environment. Furthermore, one or more flavor regulators and sweeteners can be added at the same time as the antibacterial agent and antioxidant according to the ratio.

[0062] The present invention also provides an application of an oral care liquid composition for oral care at night. Specifically, after brushing teeth before going to bed, pour 50-150 ml of the oral care liquid into the mouth, rinse the mouth gently, and allow the liquid to fully contact the teeth, gums and tongue in the mouth for 30 seconds to 1 minute. After rinsing the mouth, spit out the care liquid.

[0063] The oral care liquid composition and the preparation method thereof provided by the present invention are described in detail below in conjunction with the examples, but they should not be construed as limiting the scope of protection of the present invention. In addition, if no specific conditions are specified in the examples of the present invention, the conventional conditions or the conditions recommended by the manufacturer are used; if the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially.

[0064] 1. Preparation of oral care liquid composition in Examples and Comparative Examples

[0065] Example 1

[0066] Inoculate Streptococcus salivarius K12 into the pre-prepared MRS medium under sterile conditions, raise the temperature to 30°C, culture overnight, activate, centrifuge and wash to remove impurities in the medium; after culturing Streptococcus salivarius K12;

[0067] Mix the culture solution containing 0.05 g of Streptococcus salivarius K12 with 0.3 g of camellia seed oil and 0.3 g of polysorbate; homogenize with ultrasound at 150 W for 20 seconds, control the temperature at 10° C., and control the humidity within the range of 50% ± 2%;

[0068] Dissolve 0.1 g of domiphene, 0.15 g of vitamin C, 0.15 g of vitamin E, 1.0 g of pineapple extract, and 0.5 g of stevioside in water, add homogenized low water activity protected probiotics, adjust the pH value to 7.0 with sodium bicarbonate, and add the remainder of deionized water to prepare 100 g of oral care solution.

[0069] Example 2

[0070] Compared with Example 1, pineapple extract and stevioside were not added, the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0071] Example 3

[0072] Inoculate Lactobacillus rhamnosus GG into the pre-prepared MRS medium under sterile conditions, raise the temperature to 30°C, culture overnight, activate, centrifuge and wash to remove impurities in the medium;

[0073] Mix the culture solution containing 0.01 g of Streptococcus salivarius K12 with 0.1 g of camellia seed oil and 0.05 g of polysorbate; homogenize with ultrasound at 150 W for 10 seconds, control the temperature at 0° C., and control the humidity within the range of 45% ± 2%;

[0074] Dissolve 0.05 g of domiphene and 0.01 g of vitamin C in water, add homogenized low water activity protected probiotics, adjust the pH value to 6.8 with sodium bicarbonate, and add the remaining amount of deionized water to prepare 100 g of oral care solution.

[0075] Example 4

[0076] Inoculate Streptococcus salivarius K12 into the pre-prepared MRS medium under sterile conditions, raise the temperature to 30°C, culture overnight, activate, centrifuge and wash to remove impurities in the medium;

[0077] The culture solution containing 0.1 g of Streptococcus salivarius K12 was mixed with 0.5 g of camellia seed oil and 0.5 g of polysorbate; the mixture was homogenized by ultrasonication at 200 W for 30 seconds, the temperature was controlled at 25° C., and the humidity was controlled within the range of 65%±2%;

[0078] Dissolve 0.2g of domiphene, 0.25g of vitamin C, 0.25g of vitamin E, 3.0g of pineapple extract, and 1.0g of stevioside in water, add homogenized low water activity protected probiotics, adjust the pH value to 7.2 with sodium bicarbonate, and add the remaining amount of deionized water to prepare 100g of oral care solution.

[0079] Example 5

[0080] Compared with Example 1, the antioxidant was replaced by 0.3 g of butylated hydroxyanisole from vitamin C and vitamin E, the antibacterial agent was replaced by phenoxyethanol from domiphene, the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0081] Example 6

[0082] Compared with Example 1, the step of homogenizing the probiotics protected by low water activity is not performed.

[0083] Comparative Example 1 (without adding antibacterial agent)

[0084] Compared with Example 1, domiphene was not added, the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0085] Comparative Example 2

[0086] Compared with Example 1, vitamin C and vitamin E were not added, the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0087] Comparative Example 3

[0088] Compared with Example 1, no camellia seed oil was added and no homogenization treatment was performed. The amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0089] Comparative Example 4

[0090] Compared with Example 1, polysorbate was not added, and the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g, and the other components and addition conditions remained unchanged.

[0091] Comparative Example 5

[0092] Compared with Example 1, 0.1 g of domiphene was replaced by 0.1 g of triclosan, and the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g. The other components and addition conditions remained unchanged.

[0093] Comparative Example 6

[0094] Compared with Example 1, 0.3 g of polysorbate was replaced by 0.3 g of sodium dodecylbenzene sulfonate, and the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g. The other components and addition conditions remained unchanged.

[0095] Comparative Example 7

[0096] Compared with Example 1, 0.3 g of camellia seed oil was replaced by 0.3 g of glycerol, and the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g. The other components and addition conditions remained unchanged.

[0097] Comparative Example 8

[0098] Compared with Example 1, 0.3 g of camellia seed oil was replaced with 1.2 g of glycerol, and the amount of deionized water added was adaptively adjusted so that the total weight of the oral care solution was 100 g. The other components and addition conditions remained unchanged.

[0099] 2. Test on the preservation effect of probiotic live bacteria

[0100] Under sterile conditions, 10 mL of sample was collected from 100 g of oral care solution of Examples 1-6 and Comparative Examples 1-8, the sample was diluted 100 times with sterile saline, 0.1 mL of the diluted sample was inoculated onto nutrient agar medium, the culture dish was gently rotated to mix evenly, and the culture dish was placed in a constant temperature incubator set at 37° C. and cultured for 48 hours.

[0101] After the culture is completed, the colonies on the culture medium are counted using a microscope and a counting plate, and the number of colonies at each dilution is recorded.

[0102] According to the dilution multiple and colony count, calculate the number of viable bacteria per milliliter of the original sample. The calculation formula is as follows: The dilution multiple is 100 times, the inoculation volume is 0.1 mL, and the collection volume is 10 mL.

[0103] The above steps were repeated at the beginning of the experiment (day 0) and on days 10, 30, 60 and 180.

[0104] Table 1 shows the number of viable bacteria in Examples 1-6 and Comparative Examples 1-8 at different times

[0105]

[0106] It can be seen that the samples of Examples 1-6 obtained according to the formula and preparation process of the present invention can maintain the activity of the probiotics at more than 80% for a long time compared with that during production. Even if Example 6 is not homogenized, the decrease in its activity is not obvious. However, if the antibacterial agent, antioxidant or low water activity protective agent is not added at the same time, or a broad-spectrum antibacterial agent, anionic surfactant, and non-specific low water activity protective agent are selected, such as Comparative Examples 1-8, the activity of the probiotics will be significantly reduced within 180 days.

[0107] 3. In vitro simulated antibacterial test

[0108] Experimental methods:

[0109] Use a ceramic 3D printer (Qiyu Technology) to 3D print bionic zirconia to create a simulated tooth model.

[0110] Using soybean agar plates as culture medium, culture mutans Streptococcus in an anaerobic environment at 37°C for 3 days. After the culture is completed, carefully pick the colony with an inoculation loop and transfer it to the pre-prepared Todd Hewitt broth medium, and culture it under anaerobic conditions at 37°C for 18 hours. After that, take out 10mL of the culture solution and add it to a culture dish containing 2mL Todd Hewitt broth medium (1% sucrose has been added to the culture medium). Place the simulated tooth model at an angle of 45° in the culture dish and continue to culture it in an anaerobic environment at 37°C for 6 hours, during which a layer of bacterial film forms on the surface of the simulated tooth model.

[0111] After the incubation, 5 mL of PBS buffer was used to gently rinse the bacterial film on the test tube simulated tooth model, and the result was a model simulating dental plaque for standby use.

[0112] 10 mL of each of the oral care solutions of Examples 1-6 and Comparative Examples 1-8 and deionized water as a blank control group were immersed in the dental plaque model and kept in a 37°C water bath for 1 minute to simulate the action of rinsing the mouth. The test oral care solution was then discarded. The following operations were performed at the first time and 24 hours later:

[0113] After adding 3 mL of PBS buffer to rinse the test tube twice, pour out the liquid, add 3 mL of sodium hydroxide (0.5 M) test solution, use a vortex oscillator to shake and disperse the remaining dental plaque, and then detect the absorbance (550 nm) of the liquid in the test tube.

[0114] Plaque removal rate = (absorbance of PBS buffer - absorbance of blank control) / absorbance of blank control

[0115] Table 2 shows the immediate plaque removal rate (%) and 24h antibacterial effect of Examples 1-6 and Comparative Examples 1-8

[0116]

[0117]

[0118] It can be seen that Examples 1-6 falling within the scope of the present invention not only have good immediate cleaning and sterilization effects, but also have good antibacterial effects in the 24-hour oral environment, and there is no obvious increase in dental plaque. In Comparative Examples 1-2, when antibacterial agents and antioxidants are lacking, the immediate antibacterial effect is significantly reduced; and in Comparative Examples 3-6, when the probiotics are not subjected to specific low water activity protection and no specific surfactants are added, it can be seen that the 24-hour long-lasting antibacterial effect is not good, that is, Examples 1-6 of the present invention can have both immediate antibacterial effects and long-lasting antibacterial effects to improve the oral environment.

[0119] The above is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A night-time oral care solution for improving the oral environment, characterized in that: Based on the total mass of the oral care solution, it contains the following components and contents: Probiotics: 0.01wt%-0.1wt%; Antimicrobial agent: 0.05wt%-0.2wt%; Antioxidant: 0.01wt%-0.5wt%; Low water activity protective agent: 0.1wt%-0.5wt%; pH regulator: 0.5wt%-2wt%; Surfactant 0.05wt%-0.5wt%; and the balance of water; The probiotics are selected from one or more of Streptococcus salivarius K12 or Lactobacillus rhamnosus GG; the number of live bacteria of the probiotics in the oral care solution is maintained at 5×10 within 180 days from the date of preparation. 7 Up to 1×10 11 CFU / mL, the survival rate of live bacteria is above 80%; Among them, the low water activity protective agent is camellia seed oil; the surfactant is a nonionic surfactant and a zwitterionic surfactant, including one or more of soybean lecithin, polysorbate, cocamidopropyl betaine, alkyl glucoside, decyl glucoside, and polyoxyethylene castor oil.

2. The night-time oral care solution according to claim 1, characterized in that: The antibacterial agent is selected from one or more of domiphene, chlorhexidine, phenoxyethanol, and parabens.

3. The night-time oral care solution according to claim 1, characterized in that: The antioxidant is selected from one or more of vitamin C, vitamin E, lipoic acid, phytic acid, caffeic acid, gallic acid, butylated hydroxyanisole and butylated hydroxytoluene.

4. The night-time oral care solution according to claim 1, characterized in that: Based on the total mass of the oral care solution, the following components and contents are also included: Fragrance modifier 0wt%-3wt%; Sweetener 0wt%-1wt%; The flavor regulator is selected from one or more of pineapple extract, menthol, limonene, and carvone; The sweetener is selected from one or more of sucralose, aspartame, stevioside or erythritol.

5. The night-time oral care solution according to claim 1, characterized in that: The probiotics are protected from low water activity by the following method: (1) mixing probiotics with a low water activity protective agent and a surfactant; (2) Perform ultrasonic homogenization at a low power of 100-200w for 10-30 seconds.

6. The night-time oral care solution according to claim 5, characterized in that: In the low water activity protection step, the temperature is controlled within the range of 0-25° C., and the humidity is controlled within the range of 45-65%.

7. The night-time oral care solution according to claim 1, characterized in that: The pH regulator is selected from one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium bicarbonate and sodium carbonate, and the pH value of the night oral care solution is between 6.5 and 7.

5.

8. A method for preparing the night-time oral care solution for improving the oral environment according to claim 1, characterized in that: The steps include: (1) Preparation of probiotics: culturing and activating the probiotic strains, collecting them by centrifugation or filtration, and washing to remove impurities in the culture medium; (2) Low water activity protection of probiotics: mixing probiotics with a low water activity protective agent and a surfactant; performing ultrasonic homogenization at a low power of 100-200w for 10-30 seconds to obtain homogenized probiotics with low water activity protection; (3) Oral care solution mixing: dissolving the antibacterial agent and antioxidant in water according to the ratio, and then adding the homogenized low water activity protected probiotics, and adding the pH adjuster to adjust the pH value of the final product to between 6.5 and 7.5, to form a night-time oral care solution for improving the oral environment.

9. A method for preparing the night-time oral care solution according to claim 8, characterized in that: In step (2), the temperature is controlled within the range of 0-25° C. and the humidity is controlled within the range of 45-65%; and In step (3), the method further includes adding one or more of a flavor regulator and a sweetener at the same time as the antibacterial agent and the antioxidant according to a ratio.

10. Use of the night-time oral care solution according to claims 1-7 for oral care at night.

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