A method for propagating a hydrangea macrophylla by stem section
By inducing adventitious roots in the mother branches of large-flowered hydrangea in a high-humidity environment and then directly planting them, the problems of long seedling time and low survival rate in potted large-flowered hydrangea propagation have been solved, realizing an efficient and rapid propagation method that improves survival rate and production efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANJING INST OF VEGETABLE SCI
- Filing Date
- 2024-12-20
- Publication Date
- 2026-05-29
AI Technical Summary
Existing methods for propagating large-flowered hydrangeas in pots have long seedling growth times, low survival rates, and high requirements for climate and operation, making them prone to rotting and increasing production costs.
A single-stage propagation method is adopted, taking advantage of the high humidity tolerance of hydrangea. Adventitious roots are induced in the mother plant branches in a high-humidity environment, and cuttings are directly planted in the cultivation substrate to root, eliminating the steps of cutting and transplanting. Slow-release fertilizer and broad-spectrum fungicide are used for treatment.
This method achieves rapid seedling growth and high survival rate for large-flowered hydrangeas, with a survival rate more than 10% higher than that of traditional methods. In particular, the survival rate is increased by more than 15% under high summer temperatures, reducing the use of agricultural materials and labor, and shortening the production time.
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Figure CN119498189B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of hydrangea cultivation technology, and in particular to a single-stage propagation method for hydrangeas. Background Technology
[0002] Large-flowered hydrangea (Hydrangea macrophylla) is a plant belonging to the genus Hydrangea in the family Saxifragaceae. It has a long history of cultivation and is a common ornamental plant for spring and summer. Large-flowered hydrangeas have large, spherical inflorescences with vibrant and varied colors, forming a compact and dense flower head, making them highly ornamental. In recent years, more and more varieties of large-flowered hydrangea have entered the market, and home gardening has gradually become popular. Therefore, large-flowered hydrangeas are increasingly favored in landscape cultivation, potted plants, and floral arrangements.
[0003] In existing technologies, potted hydrangeas generally employ a two-stage cultivation method: first, propagation by cuttings, and then transplanting into pots. Cuttings are taken from softwood cuttings, resulting in a long seedling development time, typically 40-50 days. Furthermore, this method is highly susceptible to climate influences, and rotting is common during the cutting process. It also demands high standards of facility conditions and operator skill; even slight negligence can lead to large-scale mortality, disrupting production plans and increasing costs. Therefore, there is an urgent need in this field for a simple, quick, and high-survival-rate method for propagating hydrangeas. Summary of the Invention
[0004] The purpose of this invention is to provide a one-stage propagation method for large-flowered hydrangeas. By utilizing the strong tolerance of large-flowered hydrangeas to high humidity environments, the root primordia and even root systems of the mother plant branches are formed. Then, the branches that are about to develop or have already developed adventitious roots are cut into cuttings and directly planted. The cuttings take root directly in the cultivation substrate, which can realize one-stage potted production of large-flowered hydrangeas. It has the advantages of simple operation, short seedling time, and high survival rate.
[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution:
[0006] This invention provides a single-stage propagation method for large-flowered hydrangeas, comprising the following steps:
[0007] (1) Fertilize and disinfect the mother plant, and disinfect the cultivation facilities;
[0008] (2) Place the fertilized and disinfected mother plant in a high-humidity environment until dot-like protrusions or adventitious roots appear on the branches of the mother plant, and then harvest the cuttings.
[0009] (3) Plant the cuttings in the cultivation substrate and manage them until they take root;
[0010] (4) After the cuttings have taken root, carry out routine management.
[0011] Preferably, the length of the branches of the mother plant in step (1) is 10-50 cm;
[0012] The temperature of the cultivation facility is 20–40°C.
[0013] Preferably, the fertilization method is to apply slow-release fertilizer to the parent plant at a standard of 10-20g / plant.
[0014] Preferably, the disinfection method is as follows: 4-6 days after fertilizing the parent plant, the parent plant is disinfected with a broad-spectrum fungicide; the broad-spectrum fungicide includes one or more of iprodione, chlorothalonil, carbendazim, and chlorobromoisocyanuric acid.
[0015] Preferably, the relative air humidity of the high-humidity environment in step (2) is 90-100%; the start time for placing the fertilized and disinfected mother plant in the high-humidity environment is 1-3 days after disinfection; and the duration for placing the fertilized and disinfected mother plant in the high-humidity environment is 17-23 days.
[0016] As a preferred method, the cuttings are harvested as follows: select mother branches with dot-like protrusions or adventitious roots, cut off 1 to 6 nodes as 1 cutting, each cutting retains 2 to 10 leaves, and each leaf retains 1 / 3 to 1 / 3 of the original leaf area.
[0017] Preferably, the cultivation substrate in step (3) comprises the following raw materials in parts by volume: 1-3 parts peat and 0.8-1.2 parts perlite.
[0018] As a preferred method, the transplanting management method is as follows: the temperature is controlled at 20-40℃; a shade net with a shading rate of 60-70% is covered above the cuttings; watering is done once every 3-7 days to maintain the moisture content of the cultivation substrate at 40-80%.
[0019] As a preferred method, the daily management method described in step (4) is as follows: apply slow-release fertilizer at a standard of 5-10g / plant; cover with a shade net with a shading rate of 60-70% from May to October; and maintain the moisture content of the cultivation substrate at 40-80%.
[0020] The beneficial effects of this invention are as follows:
[0021] 1. The propagation method of this invention enables one-stage potted production of large-flowered hydrangeas. Treated cuttings are directly planted in the cultivation substrate, where they root and grow, eliminating the need for stem cutting propagation. It also eliminates the need for seed trays, substrates, and other agricultural inputs required for stem cutting propagation, reducing labor costs. More importantly, the survival rate of large-flowered hydrangeas using this propagation method is over 90%, more than 10% higher than that of stem cutting propagation. Especially during the high temperatures of summer, stem cutting propagation is prone to rotting, leading to a significant drop in survival rate. This propagation method ensures a high survival rate even in summer, 15% higher than that of stem cutting propagation. It also has a significant effect on improving the survival rate of large-flowered hydrangea varieties that are difficult to root, with a survival rate more than 15% higher than that of stem cutting propagation.
[0022] 2. The propagation method of this invention eliminates the step of transplanting cuttings, so the root system does not need to adapt to the new cultivation substrate and growth environment again. There is no recovery period after seedling establishment. Within the same time frame, the plant's growth indicators, such as plant height, above-ground dry weight, and underground dry weight, are significantly better than those of conventional methods. This shortens the cultivation time for finished flowers, saving production costs and allowing for faster market response. In summary, the propagation method of this invention reduces the difficulty of hydrangea propagation, increases the survival rate of propagated plants, reduces the use of agricultural inputs, greenhouses, and labor, and increases yield, making it an effective means to drive the development of the hydrangea industry. Attached Figure Description
[0023] Figure 1 These are mother plant branches that developed dot-like protrusions after treatment;
[0024] Figure 2 The trimmed cuttings;
[0025] Figure 3 Cuttings for rooting;
[0026] Figure 4 To illustrate the effects of different cultivation methods on the growth of large-flowered hydrangeas, the varieties from top to bottom are 'Endless Summer', 'Miss Saori', and 'Red Lila'; for each variety, the three plants on the left are examples, and the three plants on the right are comparative examples. Detailed Implementation
[0027] This invention provides a single-stage propagation method for large-flowered hydrangeas, comprising the following steps:
[0028] (1) Fertilize and disinfect the mother plant, and disinfect the cultivation facilities;
[0029] (2) Place the fertilized and disinfected mother plant in a high-humidity environment until dot-like protrusions or adventitious roots appear on the branches of the mother plant, and then harvest the cuttings.
[0030] (3) Plant the cuttings in the cultivation substrate and manage them until they take root;
[0031] (4) After the cuttings have taken root, carry out routine management.
[0032] In this invention, the length of the branches of the mother plant in step (1) is preferably 10-50cm, more preferably 20-40cm, and even more preferably 28-32cm; the temperature of the cultivation facility is preferably 20-40℃, and more preferably 28-32℃.
[0033] In this invention, the preferred method of fertilization in step (1) is to apply slow-release fertilizer to the parent plant at a standard of 10-20g / plant, more preferably 12-17g / plant, and even more preferably 14g / plant; the N-P2O5-K2O ratio of the slow-release fertilizer is 14-14-14.
[0034] In this invention, the disinfection method in step (1) is preferably: disinfecting the mother plant with a broad-spectrum fungicide 4 to 6 days after fertilizing the mother plant, and more preferably 5 days after fertilizing the mother plant; the broad-spectrum fungicide includes one or more of iprodione, carbendazim, chlorothalonil and chlorobromoisocyanuric acid.
[0035] In this invention, the relative air humidity of the high-humidity environment in step (2) is preferably 90-100%, more preferably 100%; the starting time for placing the fertilized and disinfected mother plant in the high-humidity environment is preferably 1-3 days after disinfection, more preferably 2 days after disinfection; the duration for placing the fertilized and disinfected mother plant in the high-humidity environment is preferably 17-23 days, more preferably 20 days.
[0036] In this invention, the method of placing the fertilized and disinfected parent plant in a high-humidity environment in step (2) is preferably as follows: when the temperature is 24-26°C every afternoon, ventilation is stopped, the facilities are closed, and water is sprayed on the parent plant for 3-5 minutes, more preferably 4 minutes, and ventilation is carried out the next day when the temperature is 29-31°C; the water spraying device is preferably a rotary nozzle with a spray radius of 0.5-1.5m and a working flow rate of 40-60L / h, more preferably a rotary nozzle with a spray radius of 1m and a working flow rate of 50L / h.
[0037] In this invention, the preferred method for harvesting cuttings in step (2) is as follows: select mother branches with dot-like protrusions or adventitious roots, cut off 1 to 6 nodes as 1 cutting, more preferably 2 to 5 nodes, leave 2 to 10 leaves for each cutting, more preferably 4 to 8 leaves, and retain 1 / 3 to 1 / 2 of the original leaf area for each leaf; the length of the cutting is preferably 5 to 7 cm, more preferably 6 cm.
[0038] In this invention, the cultivation substrate in step (3) preferably includes the following raw materials in parts by volume: 1 to 3 parts of peat, more preferably 1.3 to 2.5 parts, even more preferably 2.0 parts, and 0.8 to 1.2 parts of perlite, more preferably 0.9 to 1.1 parts, even more preferably 1.0 parts.
[0039] In this invention, the planting management method described in step (3) is preferably as follows: the temperature is controlled at 20-40℃, more preferably 24-36℃, and even more preferably 29-31℃; a shade net with a shading rate of 60-70% is covered above the cuttings, more preferably 65%; watering is preferably once every 3-7 days, more preferably once every 4-6 days, and even more preferably once every 5 days, maintaining the moisture content of the cultivation substrate at 40-80%, more preferably 50-70%, and even more preferably 64-69%.
[0040] In this invention, the routine management method described in step (4) is preferably as follows: applying slow-release fertilizer at a standard of 5-10g / plant, more preferably 7g / plant; the ratio of N-P2O5-K2O in the slow-release fertilizer is preferably 14-14-14; covering with a shade net with a shading rate of 60-70% from May to October, more preferably 65% shade net; maintaining the moisture content of the cultivation substrate at 40-80%, more preferably 50-70%, and even more preferably 64-69%.
[0041] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0042] Three cultivation comparison trials were conducted on May 10, June 10, and July 10, 2024. The comparison was the conventional two-stage propagation method for large-flowered hydrangeas, while the examples were the one-stage propagation method for large-flowered hydrangeas of this invention. The large-flowered hydrangea varieties used for cuttings were 'Endless Summer', 'Miss Saori', and 'Red Lila'. Among them, 'Endless Summer' and 'Miss Saori' were easy to root from cuttings with high survival rates, while 'Red Lila' was difficult to root from cuttings, with a lower survival rate than the other two varieties.
[0043] The dot-like protrusions described in this invention are: the formation of root primordia induced by a high-humidity environment, the root primordia continue to divide and grow, differentiating into growth points and root caps, the growth points continue to grow and the substances secreted by the root caps dissolve the epidermal cells, and thus dot-like protrusions appear on the branches, which then develop into adventitious roots.
[0044] Dense dot-like protrusions: Ten or more dot-like protrusions appear on a section of branch.
[0045] A few punctate protrusions: 1 to 5 punctate protrusions appear sporadically on a branch.
[0046] The slow-release fertilizer used in the embodiments, examples and comparative examples of this invention was purchased from Shanghai Yongtong Ecological Engineering Co., Ltd., and the N-P2O5-K2O ratio in the slow-release fertilizer is 14-14-14.
[0047] The iprodione-chlorothalonil fumigant used in the embodiments, examples and comparative examples of this invention was purchased from Shandong Xinxing Pesticide Co., Ltd. The total effective ingredient content of the iprodione-chlorothalonil fumigant was 15%, the effective ingredient content of chlorothalonil was 12%, and the effective ingredient content of iprodione was 3%.
[0048] Example
[0049] Thirty healthy, uniformly growing plants of each variety were selected as mother plants. Each plant was treated with 14g of slow-release fertilizer. Five days later, the mother plants were treated with a fumigant of iprodione and chlorothalonil, using 300g of the fumigant per acre. The treatment process was as follows: every afternoon when the temperature reached 25℃, the greenhouse was closed, and the mother plants were sprayed with water for 4 minutes. The next day, ventilation was carried out when the temperature reached 30℃. After 20 days of treatment, dot-like protrusions appeared on the branches of the mother plants. Figure 1 Cut the mother plant branches with dotted protrusions to obtain cuttings: take the top 4-6 nodes as one cutting, each cutting retains 6-8 leaves, each leaf retains 1 / 3-1 / 3 of its original leaf area, and each cutting is 6cm long. Prune 216 cuttings, and then... Figure 2 The cuttings were planted in 10cm diameter pots using a 2:1 volume ratio of peat moss and perlite as the growing medium. After planting, they were placed in a greenhouse, with 72 cuttings per plot, for a total of three plots. After planting, the cuttings were covered with a 60-70% shade net, and the temperature was controlled at 20-35℃, maintaining the moisture content of the growing medium at 64-69%. The cuttings rooted 10 days after planting. Figure 3 After rooting, apply 7g of slow-release fertilizer to each pot. 40 days after transplanting, collect data on survival rate and plant growth. The experimental results are shown below. Figure 4 The data is shown in Table 1 on the left side of the middle section.
[0050] Comparative Example
[0051] Thirty healthy, uniformly growing plants of each variety were selected as mother plants. Each plant was treated with 14g of slow-release fertilizer. Five days later, the mother plants were treated with a fumigant of iprodione and chlorothalonil at a rate of 300g per acre. Cuttings were taken from the mother plants, with the top 4-6 nodes forming one cutting. Each cutting retained 6-8 leaves, with each leaf retaining 1 / 3 to 1 / 3 of its original leaf area. Each cutting was 6cm long. The cuttings were soaked in rooting solution for 5 minutes before being inserted into the substrate. The rooting solution consisted of 10mg / L naphthaleneacetic acid + 10mg / L indolebutyric acid + 0.1mg / L triacontanol. The rooting substrate was a mixture of coconut coir and perlite in a 2:1 volume ratio. Three 72-cell trays were used for cuttings. Water was sprayed 2-4 times daily after planting. The cuttings were transplanted after 40 days. The planting substrate was a mixture of peat moss and perlite in a 2:1 volume ratio. After planting, 7g of slow-release fertilizer was applied to each pot. After planting, the pots were covered with a shade net providing 60-70% shade, and the temperature was controlled at 20-35℃, maintaining a substrate moisture content of 64-69%. Survival rate and plant growth data were collected 20 days after planting, along with the data from the previous example. The experimental results are shown below. Figure 4 The data is shown in Table 1 on the right side of the middle section.
[0052] Table 1. Effects of different cultivation methods on the survival and growth of *Hydrangea macrophylla*
[0053]
[0054]
[0055] As shown in Table 1, the survival rate of the comparative examples was relatively high (77.77%–84.72%) at suitable times (May 10th and June 10th), but decreased significantly (56.94%–75.46%) under high temperatures (July 10th). In contrast, the survival rate of the examples exceeded 90% in different batches, which was more than 10% higher than that of the comparative examples, and the survival rate under high summer temperatures was more than 15% higher than that of the comparative examples. For varieties that are not easy to root ('Red Lila'), the survival rate of the examples was more than 15% higher than that of the comparative examples. The plant height, above-ground fresh weight, and underground fresh weight of different batches of the examples were all higher than those of the comparative examples, and the plants grew faster.
[0056] Experimental Example
[0057] On June 10th, using 'Miss Saori' as the cutting material, an experiment was conducted to investigate the effect of different watering treatment durations on the survival rate of cuttings. Five treatment groups were established: watering for 14, 17, 20, 23, and 26 days. After treatment, the cuttings were pruned, their condition was observed, and they were then planted. The survival rate was recorded 20 days after planting. Each treatment group consisted of 20 mother plants, with 60 plants planted. The results are shown in Table 2.
[0058] Table 2. Effects of different water spraying durations on the survival rate of cuttings.
[0059] Processing time (d) Scion status Survival rate of cuttings (%) 14 No obvious dot-like protrusions or adventitious roots were observed on the branches. 81.67 17 A few punctate protrusions appeared on the branches, but no adventitious roots were observed. 91.67 20 The branches show dense, dot-like protrusions, but no adventitious roots are observed. 93.33 23 The branches show dense dot-like protrusions and a small number of adventitious roots. 93.33 26 The branches develop dense, dot-like protrusions and adventitious roots. 91.67
[0060] As shown in Table 2, although no dot-like protrusions were observed on the mother plant branches after 14 days of treatment, root primordia had already formed, and these cuttings had a high survival rate. After 20 days of treatment, obvious dot-like protrusions appeared on the branches, and the survival rate of the cuttings exceeded 90%, which can be regarded as the optimal time.
[0061] As can be seen from the above embodiments, the present invention provides a one-stage propagation method for large-flowered hydrangea, including: fertilizing and disinfecting the mother plant, disinfecting the cultivation facility; placing the fertilized and disinfected mother plant in a high-humidity environment until dot-like protrusions or adventitious roots appear on the branches of the mother plant, and harvesting the cuttings; transferring the rooted cuttings to routine management, etc. This propagation method cuts branches that are about to or have already produced adventitious roots into cuttings and directly planted them. The cuttings root directly in the cultivation substrate, which can eliminate the steps of cutting propagation and transplanting of cuttings, and there is no seedling recovery period after transplanting, resulting in faster plant growth. Furthermore, the survival rate of this propagation method is over 90%, which is more than 10% higher than that of cutting propagation; this method can ensure that the survival rate of large-flowered hydrangea remains at a high level in summer, which is more than 15% higher than that of cutting propagation; this method can also improve the survival rate of varieties that are not easy to root, which is more than 15% higher than that of cutting propagation.
[0062] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for propagating large-flowered hydrangeas in a single stage, characterized in that, Includes the following steps: (1) Fertilize and disinfect the mother plant, and disinfect the cultivation facilities; (2) Place the fertilized and disinfected mother plant in a high-humidity environment until dot-like protrusions or adventitious roots appear on the branches of the mother plant, and then harvest the cuttings; (3) Plant the cuttings in the cultivation substrate and manage them until they take root; (4) After the cuttings have rooted, carry out routine management; The duration of placing the fertilized and disinfected parent plants in a high-humidity environment as described in step (2) is 17-23 days; The relative humidity of the high-humidity environment mentioned in step (2) is 90~100%; the time for placing the fertilized and disinfected mother plant in the high-humidity environment is 1~3 days after disinfection; The method for harvesting cuttings in step (2) is as follows: select mother branches with dot-like protrusions or adventitious roots, cut off 1 to 6 nodes as 1 cutting, leave 2 to 10 leaves for each cutting, and retain 1 / 3 to 1 / 3 of the original leaf area for each leaf.
2. The breeding method according to claim 1, characterized in that, The length of the branches of the mother plant in step (1) is 10~50 cm, and the temperature of the cultivation facility is 20~40℃.
3. The breeding method according to claim 2, characterized in that, The fertilization method described in step (1) is to apply slow-release fertilizer to the mother plant at a standard of 10~20 g / plant.
4. The breeding method according to claim 3, characterized in that, The disinfection method described in step (1) is as follows: 4-6 days after fertilizing the mother plant, a broad-spectrum fungicide is used to disinfect the mother plant; the broad-spectrum fungicide includes one or more of iprodione, chlorothalonil, carbendazim and chlorobromoisocyanuric acid.
5. The breeding method according to claim 1, characterized in that, The cultivation substrate mentioned in step (3) includes the following raw materials in the following volume parts: 1-3 parts peat and 0.8-1.2 parts perlite.
6. The breeding method according to claim 5, characterized in that, The planting management method described in step (3) is as follows: the temperature is controlled at 20~40℃; a shade net with a shading rate of 60~70% is covered above the cuttings; water is applied once every 3~7 days to maintain the moisture content of the cultivation substrate at 40~80%.
7. The breeding method according to claim 6, characterized in that, The daily management method described in step (4) is as follows: apply slow-release fertilizer at a standard of 5~10g / plant; cover with shade net with a shading rate of 60~70% from May to October; and maintain the moisture content of the cultivation substrate at 40~80%.