A pesticide composition

Abstract

The present invention belongs to the technical field of pesticides and insecticides and relates to a pesticide composition and its use. The pesticide composition comprises active ingredients A and B, wherein active ingredient A is a compound of formula (I) and active ingredient B is isoflualanam or cybenzoxasulfyl. The mass ratio of active ingredient A to active ingredient B is 1:45 to 27:1. The composition has the advantages of enhancing synergy and delaying resistance development, and is used for controlling resistant pests.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and in particular relates to a pesticide composition. Background Art

[0002] Isoflualanam (oxazolidinone) is an isoxazoline insecticide and an allosteric modulator of γ-aminobutyric acid (GABA)-gated chloride channels. It has a unique mechanism of action and exhibits no cross-resistance with other insecticides. Isoflualanam exhibits stomach toxicity, contact toxicity, and some systemic activity, is highly fast-acting, and has ovicidal properties. Its chemical structure is as follows:

[0003]

[0004] Cybenzoxasulfyl (oxazolidinone) belongs to the benzoxazole class of insecticides and has a broad insecticide spectrum. Its chemical structure is as follows:

[0005]

[0006] In agricultural production, the control of various harmful insects relies primarily on the application of large quantities of pesticides and insecticides. The frequent and irrational use of these pesticides has led to the continuous development of insect resistance, exacerbating the damage to crops and making their control increasingly difficult. By combining the compound of formula (I) with two or more pesticides with different mechanisms of action, such as isoflualanam and cybenzoxasulfyl, it is possible to simultaneously control different pests, broaden the control spectrum, enhance the efficacy of the pesticides, and mitigate the development of pest resistance. Summary of the Invention

[0007] The purpose of the present invention is to provide a pesticide composition which has synergistic effects, reduces resistance and has low use cost.

[0008] To achieve the above object, the technical solution adopted by the present invention is: a pesticide composition comprising active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) The active ingredient B is Isoflualanam or Cybenzoxasulfyl;

[0009] Furthermore, the mass ratio between the active ingredient A and the active ingredient B is 1:45 to 27:1.

[0010] Furthermore, the mass ratio of the compound of formula (I) to Isoflualanam is 1:35 to 27:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to Isoflualanam is 1:35 to 18:1 or any value between the above values;

[0012] Furthermore, the mass ratio of the compound of formula (I) to Isoflualanam is 1:35, 1:22, 1:8, 1:3, 3:1, 10:1, 18:1, and 27:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to Isoflualanam is 1:35, 1:22, 1:8, 1:3, 3:1, 10:1, and 18:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:45 to 25:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:30 to 25:1 or any value between the above values;

[0016] Furthermore, the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:45, 1:30, 1:20, 1:10, 2:9, 9:2, 12:1, and 25:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:30, 1:20, 1:10, 2:9, 9:2, 12:1, and 25:1;

[0018] Furthermore, based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%;

[0019] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1% to 85%;

[0020] Furthermore, the pesticide composition may include, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist, or a carrier;

[0021] Furthermore, the acaricidal composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0022] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0023] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0024] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0025] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0026] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0027] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0028] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0030] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0031] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0032] Synergists are selected from synergist, piperonyl butoxide; and / or

[0033] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0034] Furthermore, the pesticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0035] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0036] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0037] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0038] Application of the pesticide composition of the present invention in preventing and controlling agricultural and forestry pests and sanitary pests;

[0039] The pesticide composition of the present invention can be used to control pests on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0040] Furthermore, the agricultural and forestry pests and sanitary pests are Lepidoptera pests;

[0041] Furthermore, the Lepidoptera pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma talis, Archips argyrospila (fruittree leafroller), Archips rose leafroller, and A. rosana (rose leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana (orange tortrix), Autographa gamma, Bonagota cranaodes, Borbo cinnara (rice leaf folder), Bucculatrix thurberiella (cotton leaf perforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peach fruit moth), Chilo spp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded rose leaf roller), leafroller)), Chrysodeixis spp.), Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borer), borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester corn borer), Earias spp.(cotton bollworm), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopopha aurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown apple moth), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbacomoth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema), Erionotathrax (banana skipper), Eupoecilia ambiguella (grapeberry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholitamolesta (Oriental fruit moth).

[0042] (Oriental Fruit Moth), Hedylepta indicate (Bean Leaf Webber), Helicoverpa sp. (Spodoptera exigua), Helicoverpa armigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (Cabbage Webworm), Indarbela spp. (Root Borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (Grape Fruit Moth), fruitmoth), Loxagrotis spp., Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.) (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana (bagworm), Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis (three-spotted water borer), Operophtherabrumata (winter looper), Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus (African swallowtail), Pectinophora gossypiella (pink bollworm), (Bollworm), Peridroma species (pp.), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp., Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays olive moth oleae) (olive moth), Pseudaletia spp.(Spodoptera litura Fabricius), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu), Chilo suppressalis (Walker), Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera frugiperda fugiperda), Spodoptera oridania (southern armyworm), Synanthedon spp., Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (cabbage worm), Pieris rapae Linne (cabbage worm), and Tuta absoluta.

[0043] Furthermore, the lepidopteran pests include diamondback moth, beet armyworm, Spodoptera litura, striped stem borer, cotton bollworm, and cabbage worm;

[0044] Furthermore, the lepidopteran pest is Spodoptera litura.

[0045] The present invention also provides a method for using the above pesticide composition, specifically applying an effective dose to the pests to be controlled or the medium in which they grow.

[0046] The pesticide composition of the present invention has the following advantages:

[0047] 1) The pesticide composition of the present invention exhibits a synergistic effect within a certain ratio range and has a significant control effect;

[0048] 2) The two active ingredients of the pesticide combination have a unique mechanism of action, are extremely effective against resistant pests, and can extend the service life of the product. DETAILED DESCRIPTION

[0049] The present invention is further described below with reference to the examples. The percentages in the examples are all by weight, but the present invention is not limited thereto.

[0050] The composition of the present invention can be provided in the form of a formulation. It can be formulated as a suspension concentrate, water-dispersible granules, aqueous emulsion, granules, wettable powder, or dispersible oil suspension, as needed. The content of the active ingredient in the composition of the present invention depends on the dosage when used alone, as well as the blending ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0051] Preparation Example

[0052] Example 1:

[0053] 5% formula (Ⅰ) compound·isoflualanam emulsifiable concentrate (1.5+3.5)

[0054] Formula: 1.5% compound of formula (I), 3.5% isoflualanam,

[0055] 4% propylene carbonate, 4% calcium dodecylbenzenesulfonate, 12% castor oil polyoxyethylene ether, 1% N,N-dimethylformamide, 2% corn oil, 30% cyclohexanone, and methyl oleate make up the balance;

[0056] Preparation method: first add the active ingredient into the solvent and dissolve it completely, then add the emulsifier and stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0057] Example 2:

[0058] 30% formula (I) compound·isoflualanam suspension (5+25)

[0059] Formula: 5% compound of formula (I), 25% isoflualanam,

[0060] 3% fatty alcohol polyoxyethylene ether phosphate, 1% sodium lignin sulfonate, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl parahydroxybenzoate, 4.5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0061] Preparation method: Add active ingredients to the wetting dispersant and defoamer, use zirconium oxide beads, and wet grind with a sand mill to D 90 (90% of the particles have a particle size of less than 10 μm) to obtain a crushed slurry. A thickener, antifreeze agent, preservative, etc. are added to the crushed slurry and mixed evenly. Deionized water is added to 100%, and the suspension product is obtained by high-speed shearing.

[0062] Example 3:

[0063] 48% formula (Ⅰ) compound·isoflualanam water dispersible granules (8+40)

[0064] Formula: 8% compound of formula (I), 40% isoflualanam,

[0065] 6% sodium polycarboxylate, 2% sodium lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 3.5% sodium lauryl sulfate, 4% sodium sulfate, 2% starch, and kaolin to make up the balance;

[0066] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, appropriate water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0067] Example 4:

[0068] 22% formula (Ⅰ) compound·cybenzoxasulfyl suspension (4+18)

[0069] Formula: 4% compound of formula (I), 18% cybenzoxasulfyl,

[0070] 3% fatty alcohol polyoxyethylene ether phosphate, 4% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% naphthalenesulfonate formaldehyde condensate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl parahydroxybenzoate, 5% propylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0071] Preparation method: same as Example 2.

[0072] Example 5:

[0073] 16% compound of formula (I)·cybenzoxasulfyl dispersible oil suspension (3+13)

[0074] Formula: 3% compound of formula (I), 13% cybenzoxasulfyl,

[0075] 1.8% naphthalenesulfonate condensate, 12% castor oil polyoxyethylene ether, 3% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 0.8% organic bentonite, and soybean oil makes up the balance;

[0076] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0077] Example 6:

[0078] 20% formula (Ⅰ) compound·cybenzoxasulfyl water dispersible granules (4+16)

[0079] Formula: 4% compound of formula (I), 16% cybenzoxasulfyl,

[0080] 5% sodium polycarboxylate, 2% sodium lignin sulfonate, 6% naphthalene sulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 2% pulverized powder BX, 2% starch, and kaolin to make up the balance;

[0081] Preparation method: same as Example 3.

[0082] Indoor biological activity assay

[0083] Refer to the agricultural industry standards "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insect Dipping Method" (NY / T1154.6-2006), "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006), "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14: Leaf Dipping Method" (NY / T 1154.14-2008), etc.

[0084] Specific indoor toxicity test:

[0085] Test target: Prodenia litura (Fabricius);

[0086] Test method: Combination of leaf dipping method and insect dipping method;

[0087] Preparation of drugs: The test drugs were dissolved in acetone and prepared into 5 series of concentrations using 0.1% Tween 80 aqueous solution.

[0088] Test materials: Use a hole punch to punch leaf discs (1.5 cm in diameter) and immerse them in the drug solution for 10 seconds. After the solution is allowed to dry naturally, place the cabbage discs in a Petri dish lined with moisturizing filter paper. Place 20 third-instar larvae in the drug solution for 5 seconds. After absorbing the excess solution with filter paper, place the test insects in a Petri dish with leaves soaked in the corresponding concentration.

[0089] Experimental treatment: Each treatment was repeated 4 times, with a blank treatment as a control. The treated insects were reared in an artificial intelligence culture chamber at 25±1°C, a light intensity of L:D=16h:8h, and a relative humidity of 65%±5%.

[0090] Survey method: After 48 hours, check the results. Use the tip of a brush to gently touch the insects. If there is no reaction, the insect is considered dead. Count the number of live and dead Spodoptera litura in each treatment and calculate the mortality rate.

[0091] Data statistics and analysis:

[0092] Based on the survey data, calculate the adjusted mortality rate of each treatment using the following formula, with the results rounded to two decimal places:

[0093]

[0094] Where:

[0095] P——mortality rate, in percentage (%);

[0096] K——indicates the number of dead insects, the unit is head;

[0097] N——represents the total number of insects processed, in heads.

[0098]

[0099] Where:

[0100] P1——adjusted mortality rate, in percentage (%);

[0101] P t ——Treatment mortality rate, expressed in percentage (%);

[0102] P0 - blank control mortality rate, in percentage (%).

[0103] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0104] The data can be processed by probability value analysis. The data statistical analysis system can be used to analyze the toxicity regression line and LC 50 The activity of the test agents on the biological materials was evaluated by using the values, 95% confidence limits and correlation coefficient r.

[0105] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0106]

[0107] Where:

[0108] ATI - measured toxicity index of mixture;

[0109] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0110] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0111] TTI=TI A *P A +TI B *P B

[0112] Where:

[0113] TTI – Theoretical Toxicity Index of Mixtures;

[0114] TI A ——Agent toxicity index;

[0115] P A ——The percentage of agent A in the mixture, in percentage (%);

[0116] TI B ——Toxicity index of agent B;

[0117] P B ——The percentage of agent B in the mixture, in percentage (%).

[0118]

[0119] Where:

[0120] CTC – Co-toxicity coefficient;

[0121] ATI - measured toxicity index of mixture;

[0122] TTI - Theoretical Toxicity Index of Mixture.

[0123] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0124] Test results:

[0125] Combined toxicity of the mixture to Spodoptera litura:

[0126] The LC of the compound of formula (I) against Spodoptera litura 50 The LC value of Isoflualanam against Spodoptera litura is 1.033 mg / L. 50The LC value of Cybenzoxasulfy against Spodoptera litura is 2.974 mg / L. 50 The compound of formula (I) mixed with isoflualanam and cybenzoxasulfy has a good combined effect on Spodoptera litura, and at a certain mass ratio, it shows obvious synergistic effect.

[0127] The results in Table 1 show that when the mass ratio of the compound of formula (I) to Isoflualanam is 1:35-27:1, the co-toxicity coefficient of the control effect on Spodoptera litura is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Isoflualanam is 1:35-18:1, the co-toxicity coefficient of the control effect on Spodoptera litura is greater than 120, showing a synergistic effect.

[0128] Table 1 Toxicity test results of different ratios of compound of formula (I) and Isoflualanam to Spodoptera litura

[0129]

[0130]

[0131] The results in Table 2 show that when the mass ratio of the compound of formula (I) to Cybenzoxasulfy is 1:45 to 25:1, the co-toxicity coefficient of the control effect on Spodoptera litura is greater than 80, showing an additive or synergistic effect. When the mass ratio of the compound of formula (I) to Cybenzoxasulfy is 1:30 to 25:1, the co-toxicity coefficient of the control effect on Spodoptera litura is greater than 120, showing a synergistic effect.

[0132] Table 2 Toxicity test results of different ratios of compound of formula (I) and cybenzoxasulfyl to Spodoptera litura

[0133]

[0134] Field efficacy examples

[0135] Field trial 1

[0136] Test crops: Radish (green radish);

[0137] Test target: Spodoptera litura;

[0138] Experimental location: Cruciferous vegetable flower planting experimental field in Shangma Street, Chengyang District, Qingdao City, Shandong Province;

[0139] Trial time: September 24, 2023;

[0140] Application method: Use a knapsack electric sprayer to apply pesticides manually according to the design plan in the test field. First spray the whole plant, then focus on spraying the back of the leaves. The water consumption is 100L / hm2. 2 .

[0141] Experimental environment: All experimental plots were uniformly cultivated, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil;

[0142] Test agent:

[0143] Table 3 Field test design and dosage

[0144]

[0145]

[0146] Test method: Apply the pesticide once during the peak period of Spodoptera litura (mostly 2nd to 4th instar).

[0147] Plot size and repetition: random block arrangement, plot size 20m 2 , each treatment was repeated 4 times.

[0148] Survey method: Randomly sample five points in each plot, hang 10 plants at each point, investigate the number of Spodoptera litura on all radishes, investigate the base number of insects before spraying, and investigate the number of remaining insects 2 days and 7 days after spraying.

[0149] Calculation formula and data analysis:

[0150]

[0151] Field efficacy test results:

[0152] The test results in Table 4 show that 2 days after application, 30% of the compound of formula (I)·isoflualanam suspension concentrate (5+25) and 16% of the compound of formula (I)·cybenzoxasulfyl dispersible oil suspension concentrate (3+13) showed good fast-acting properties against Spodoptera litura, with control efficacy of 87.22% and 88.41%, respectively, which were significantly higher than the control single agent; 7 days after application, 30% of the compound of formula (I)·isoflualanam suspension concentrate (5+25) and 16% of the compound of formula (I)·cybenzoxasulfyl dispersible oil suspension concentrate (3+13) showed good persistent effects against Spodoptera litura, with control efficacy of 90.16% and 91.46%, respectively, which were significantly higher than the control single agent.

[0153] Table 4 Field control effects of different treatment agents on cauliflower worms

[0154]

[0155] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0156] Safety: During the trial period and subsequent observations, radish grew normally and no obvious phytotoxicity was observed.

[0157] The pesticide composition or formulation obtained by the present invention exhibits significant pest control efficacy, outperforming single-dose formulations in delaying the development of pesticide resistance and prolonging insecticide retention. Furthermore, no pesticide damage to crops was observed in the experiments, demonstrating that the pesticide composition or formulation, while enhancing insecticide synergy, can reduce production and use costs and is safe for crops.

[0158] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: It includes active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) , the active ingredient B is Isoflualanam or Cybenzoxasulfyl; the mass ratio of the compound of formula (I) to Isoflualanam is 1:35~18:1; the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:30~25:

1.

2. The pesticide composition according to claim 1, wherein: The mass ratio of the compound of formula (I) to Isoflualanam is 1:35, 1:22, 1:8, 1:3, 3:1, 10:1, and 18:1; the mass ratio of the compound of formula (I) to Cybenzoxasulfyl is 1:30, 1:20, 1:10, 2:9, 9:2, 12:1, and 25:

1.

3. The pesticide composition according to claim 1, wherein: Based on the total mass of the pesticide composition as 100wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%.

4. The pesticide composition according to claim 1, wherein: In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The pesticide composition according to claim 4, characterized in that: The pesticide composition can be prepared into an agriculturally acceptable formulation, and the formulation is selected from a solid formulation and / or a liquid formulation.

6. The pesticide composition according to claim 5, characterized in that: The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

7. Use of the pesticide composition according to any one of claims 1 to 6 in preventing and controlling agricultural and forestry pests and sanitary pests.

8. The use according to claim 7, characterized in that: The agricultural and forestry pests and sanitary pests are lepidoptera pests.

9. The pesticide composition according to claim 1, wherein: The pesticide composition is applied to the pests to be controlled or the medium where the pests grow at an effective dose.

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