Suyun • Rice Leaffolder Suspending Agent and Its Preparation and Application Method

Abstract

The present invention belongs to the technical field of agricultural microbial preparations. The Suyun Rice Granular Suspension Concentrate comprises a Bacillus thuringiensis concentrate and a rice leaf roller granule virus suspension in a volume ratio of 1000:(1-2); the Bacillus thuringiensis concentrate has a Bacillus thuringiensis bioavailability of ≥16,000 IU / ml; and the rice leaf roller granule virus suspension has a rice leaf roller granule virus content of ≥100,000 OB / ml. The present invention addresses the technical problem that existing commonly used biopesticide formulations cannot meet the requirements of unmanned aerial pest control. The present invention also discloses a method for preparing the Suyun Rice Granular Suspension Concentrate. The present invention also discloses a method for using the Suyun Rice Granular Suspension Concentrate.

Description

Technical Field

[0001] The present invention belongs to the technical field of agricultural microbial preparations, and specifically relates to a Suyun•Rice granular suspension concentrate, a preparation method of the suspension concentrate, and an application method of the suspension concentrate. Background Art

[0002] With the implementation of the zero-growth campaign for pesticide use, drone-based plant protection operations have experienced rapid growth. Drone-based plant protection offers significant advantages in operational efficiency, pesticide and water conservation, and its effectiveness is gradually surpassing that of traditional application methods. The National 14th Five-Year Plan explicitly calls for the development of new varieties and formulations of highly effective, low-risk green pesticides, centered around the needs of specialized pest and disease control and aerial plant protection. This will continue to promote pesticide reduction and support national food security, agricultural product quality safety, and ecological and environmental safety. Currently, production still lacks specialized biopesticide formulations suitable for drone-based plant protection. Conventional pesticide formulations are commonly used, with high-concentration dilutions or undiluted ultra-low-volume spraying.

[0003] Bacillus thuringiensis (Bt) is the most widely used and most widely used biopesticide, exhibiting varying degrees of pathogenicity against over 500 insect species, including the rice leaf roller, and plays a crucial role in the integrated management of pests in agriculture, forestry, public health, and storage. However, research on biopesticide formulations is relatively underdeveloped. Existing Bacillus thuringiensis formulations use corn flour and soybean meal as their primary carbon and nitrogen sources, respectively. These high levels of agricultural and sideline products contain significant amounts of cellulose, making them difficult to sieve and meet the requirements of unmanned aerial spraying. Therefore, a soluble formulation is urgently needed to prepare biopesticides. Some commonly used biopesticide formulations, such as wettable powders, have large particle sizes and high particle sedimentation coefficients. These formulations are prone to aggregation, settling, and nozzle clogging during ultra-low-volume spraying, making them difficult to directly apply to aerial spraying operations. Therefore, it is necessary to modify the formulation and employ processes such as screening and centrifugation to improve efficacy and reduce usage. Rice leaf folder granulovirus is an obligate baculovirus that infects the rice leaf folder. It can sustainably spread in natural populations of the pest, providing persistent effectiveness in field control and offering a new approach to addressing pesticide residues and serious non-point source pollution in rice fields. The team's previous research on the Suyun Rice Granules wettable powder (WP) has shown that an application of 100g per mu can effectively control damage from the WP. However, due to the limitations of the WP formulation, it cannot meet the requirements of unmanned aerial control. Summary of the Invention

[0004] The first purpose of the present invention is to provide a Suyun•Rice granular suspension concentrate to solve the technical problem that the existing commonly used biological pesticide formulations cannot meet the requirements of unmanned aerial spraying.

[0005] To solve the above technical problems, the present invention adopts the following technical solution: Suyun•Rice granular suspension concentrate, characterized in that it comprises a Bacillus thuringiensis concentrate and a rice leaf roller granular virus suspension, wherein the Bacillus thuringiensis concentrate has a Bacillus thuringiensis bioavailability of ≥16,000 IU / ml;

[0006] The rice leaf roller granule virus suspension has a rice leaf roller granule virus content of ≥100,000 OB / ml;

[0007] The volume ratio of the Bacillus thuringiensis concentrated solution to the rice leaf roller granule virus suspension is 1000:(1-2).

[0008] This invention combines Bacillus thuringiensis concentrate with a rice leaf roller granule virus and adjuvants, effectively overcoming the weaknesses of Bacillus thuringiensis single-dose control, including low efficacy and slow-acting viral preparations, while also improving its performance in drone-based control. The Suyun Rice Granular Suspension Concentrate prepared in this invention is suitable for drone-based control, effectively controlling the damage caused by the rice leaf roller. The suspension formulation of this invention is suitable for drone-based control, effectively controlling the damage caused by the rice leaf roller and protecting its natural enemy.

[0009] To further address the technical issue of suspending agents being suitable for drone application, the present invention employs the following technical solution: the Suyun Rice Granular SC also includes adjuvants, including sodium benzoate and organosilicon. The sodium benzoate accounts for 0.2-0.4% by weight of the Suyun Rice Granular SC, and the organosilicon accounts for 0.1-0.3% by weight of the Suyun Rice Granular SC. Adding these to the SC improves its drone application performance.

[0010] Rice leaf folders were reared in the laboratory and inoculated with C. medinalis granulovirus. Dead insect carcasses were collected and the virus preparation was made into a 1000 million / ml C. medinalis granulovirus suspension. A 16,000 IU / ml Bacillus thuringiensis concentrate and a 1000 million / ml C. medinalis granulovirus suspension were mixed in a ratio of 1:2. 0.2%-0.4% sodium benzoate was added to the mixture, the pH was adjusted to 4.0-5.0, and 0.1%-0.3% silicone was added to complete the preparation of the Thuringiensis Rice Granular Suspension Concentrate.

[0011] The second object of the present invention is to provide a method for preparing a suspending concentrate of Suyun Rice Granules, comprising:

[0012] fermenting Bacillus thuringiensis spore liquid using a soluble culture medium to obtain a fermentation product;

[0013] The fermented product was passed through a vibrating sieve to collect the Bacillus thuringiensis liquid;

[0014] The Bacillus thuringiensis liquid is concentrated by centrifugation to obtain a Bacillus thuringiensis concentrated liquid;

[0015] Add the formulated amount of rice leaf roller granule virus to the Bacillus thuringiensis concentrate and mix evenly. Add adjuvants and adjust the pH value to 4.0-6.0 to obtain the Bacillus thuringiensis rice granule suspension concentrate.

[0016] This invention prepares for the first time a high biological efficacy Suyun•Rice vertical granular suspension concentrate. By screening a soluble formula of Bacillus thuringiensis, reducing the insoluble particles in the culture medium, and further utilizing disc centrifugal concentration processing, integrating granular viruses and adjuvants, a new type of Suyun•Rice vertical granular aerial pest control preparation with high content, low dilution, stable suspension, and dispersed sedimentation is developed, which can effectively promote the promotion and application of biological pesticides.

[0017] In order to solve the technical problem of how to realize the soluble culture medium, the present invention adopts the following technical solution, wherein the soluble culture medium comprises:

[0018] Soy peptone 1wt%-2.5wt%, corn starch 1wt%-1.5wt%, calcium carbonate 0.05wt%-0.1wt%, dipotassium hydrogen phosphate 0.05wt%-0.1wt%, sodium chloride 0.1wt%-0.2wt%, water 95.6%-97.8wt%.

[0019] The present invention screens a soluble formula suitable for the proliferation of Bacillus thuringiensis. The formula can quickly complete the fermentation of Bacillus thuringiensis, has high uniformity and biological potency, and has significant economic benefits.

[0020] In order to solve the technical problem of how to prepare a fermentation product, the present invention adopts the following technical solution, wherein the fermentation product is obtained by fermenting Bacillus thuringiensis spore liquid using a soluble culture medium, comprising:

[0021] The soluble culture medium was added to the fermentation tank, sterilized at 121°C for 30 min, and then cooled to 34°C, and then the Bacillus thuringiensis spore liquid was added for fermentation. The fermentation control culture conditions were: rotation speed 180-250 rpm / min, temperature 30-34°C, ventilation 3 m 3 / min, the fermentation tank pressure is maintained at 0.3-0.4MPa;

[0022] The fermentation culture is continued for 35-45 hours until more than 90% of the crystals are released and the pH value is 8.0 to obtain a fermentation product.

[0023] In order to solve the technical problem of how to realize the vibration screen, the present invention adopts the following technical solution, and the screen of the vibration screen is 200 meshes.

[0024] To solve the technical problem of how to concentrate a Bacillus thuringiensis liquid, the present invention adopts the following technical solution: the Bacillus thuringiensis liquid is centrifuged and concentrated by 5-10 times using a disc centrifuge, and a concentrated liquid is collected, wherein the Bacillus thuringiensis bioavailability in the concentrated liquid is ≥16,000 IU / ml; the rotation speed of the disc centrifuge is 4,000-6,000 rpm / min.

[0025] The invention prepares a Bacillus thuringiensis concentrated liquid with a biological potency of ≥16000 IU / ml from a Bacillus thuringiensis liquid through disc centrifugation, thereby reducing the amount of subsequent preparations per mu.

[0026] In order to solve the technical problem of how to prepare a suspension concentrate, the present invention adopts the following technical solution: a formulated amount of rice leaf roller granule virus is added to the Bacillus thuringiensis concentrate, an adjuvant is added, and the pH value is adjusted to 4.0-6.0, comprising:

[0027] The Bacillus thuringiensis concentrate and the rice leaf roller granule virus suspension are evenly mixed, 0.2% to 0.4 wt% of sodium benzoate is added to the mixture, the pH value is adjusted to 4.0 to 5.0 with sulfuric acid, and 0.1% to 0.3 wt% of organosilicon is added.

[0028] The third object of the present invention is to provide an application method of the above-mentioned Suyun•Rice Vertical Granule Suspension Concentrate. During the peak hatching period of rice leaf roller eggs, the Suyun•Rice Vertical Granule Suspension Concentrate is diluted 10 times, and 100~150 ml of the Suyun•Rice Vertical Granule Suspension Concentrate is used per acre of rice field. The spraying is carried out evenly according to the standard parameters of drone flight control, which can effectively control the damage caused by rice leaf roller.

[0029] Suyun Rice Suspension Concentrate (SC) is a biopesticide specifically targeting the rice leaf folder (Cnaphalocrocis medinalis). It is safe against natural enemies and helps maintain the stability of rice paddy ecosystems. The application of SC can effectively advance the industrialization of granular viral insecticides against rice leaf folders, reduce the use of chemical pesticides, and curb outbreaks of the insect.

[0030] The occurrence of rice leaf folders in the control area and the control area was investigated 7 days and 14 days after application. The results showed that the corrected mortality rate of rice leaf folders reached 89% 7 days after application of Suyun Rice Granules, and the corrected mortality rate was still 77% after 14 days, indicating that it can effectively control the damage caused by rice leaf folders and is an ideal biological pesticide for controlling rice leaf folders.

[0031] Preferably, the standard parameters for drone spraying are a flight altitude of 1.5 to 2 m, a nozzle flow rate of 6 L / min, and a spray width of 4 m. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a diagram showing the crystal release during the late stage of Bacillus thuringiensis cultivation;

[0033] Figure 2 FIG is a diagram of the Bacillus thuringiensis liquid prepared according to the present invention;

[0034] Figure 3 This is a diagram of the proliferation of the rice leaf roller granulovirus of the present invention;

[0035] Figure 4a This is a schematic diagram of a drone for spraying the Suyun•Rice Granular Suspension Concentrate of the present invention;

[0036] Figure 4b This is the spray characteristic diagram of the drone of the present invention;

[0037] Figure 5 This is a diagram showing the deposition of the Suyun•Rice Granules suspension concentrate (left) and Suyun•Rice Granules wettable powder (right) on the filter screen of a drone;

[0038] Figure 6 This is an analysis diagram of the use effect of the Suyun·Rice Granular Suspension Concentrate of the present invention. DETAILED DESCRIPTION

[0039] The present invention will be further described below with reference to the accompanying drawings.

[0040] In order to better understand the present invention, the present invention will be further described below by way of examples, but the scope of the present invention is not limited thereto. Unless otherwise specified, the reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art.

[0041] Example 1: Screening of Bacillus thuringiensis soluble culture medium formulations

[0042] The proportions of the components of the basic culture medium are: soy peptone 1%, corn starch 1%, calcium carbonate 0.05%, dipotassium hydrogen phosphate 0.1%, sodium chloride 0.2%, and water 97.65%.

[0043] The culture conditions of Bacillus thuringiensis were as follows: rotation speed 220 rpm / min, temperature 34°C.

[0044] The method for determining the biological activity of the culture broth is as follows: the fermentation broth is diluted to five concentrations of 10µL / mL, 5µL / mL, 2.5µL / mL, 1.25µL / mL, and 0.625µL / mL, and 16m 2 Cabbage leaves were immersed in the diluted solution, dried, and placed in petri dishes. Ten third-instar larvae of the diamondback moth were seeded into each dish, with each treatment replicated three times. After two days of rearing at 28°C with a light:dark ratio of 16:8, the larval mortality rate was assessed, and the median lethal concentration and bioavailability were calculated.

[0045] (1) Selection of nitrogen source

[0046] Remove soy peptone from the basal culture medium, select soy peptone, fish meal peptone, and bovine bone peptone as nitrogen sources, each added at 1%, and then inoculate 1 bacterium of Bacillus thuringiensis spores. After normal culture for 42 hours, wait until the crystals are completely released (such as Figure 1 ) was used to determine the bioavailability of the fermentation broth. Testing revealed that the bioavailability of fermentation broth using soy peptone as a nitrogen source was 3482.4 IU / mL, higher than the 3089.4 IU / mL and 1791.9 IU / mL values of fish meal peptone and bovine bone peptone, respectively (Table 1). Therefore, soy peptone is a preferred nitrogen source for Bacillus thuringiensis fermentation.

[0047] Table 1 shows the toxicity test results of different nitrogen source addition groups on diamondback moth larvae

[0048]

[0049] (2) Selection of carbon source

[0050] Remove corn starch from the basal culture medium, select corn starch, corn steep liquor, and yeast extract as carbon sources, add 1% of each, and then inoculate 1 bacterium of Bacillus thuringiensis spores. After normal culture for 42 hours, wait until the crystals are completely released (such as Figure 1 ) was used to determine the bioavailability of the fermentation broth. Testing revealed that the bioavailability of the fermentation broth using corn starch as a carbon source was 3426.9 IU / mL, higher than the 1999.2 IU / mL and 2583.7 IU / mL values obtained using corn steep liquor and yeast extract as carbon sources, respectively (Table 2). Therefore, corn starch is a preferred carbon source for Bacillus thuringiensis fermentation.

[0051] Table 2 shows the toxicity test results of different carbon source addition groups on diamondback moth larvae

[0052] (3) Determination of the content of each component

[0053] The proportions of the basal culture medium components were adjusted. Experimental Group 1 consisted of: 1.2% soy peptone, 1% corn starch, 0.05% calcium carbonate, 0.1% dipotassium hydrogen phosphate, 0.2% sodium chloride, and 97.45% water. Experimental Group 2 consisted of: 1.5% soy peptone, 1% corn starch, 0.05% calcium carbonate, 0.1% dipotassium hydrogen phosphate, 0.2% sodium chloride, and 97.15% water. Experimental Group 3 consisted of: 1.7% soy peptone, 1% corn starch, 0.05% calcium carbonate, 0.1% dipotassium hydrogen phosphate, 0.2% sodium chloride, and 96.95% water. Experimental Group 4 consisted of: 1.5% soy peptone, 1.2% corn starch, 0.05% calcium carbonate, 0.1% dipotassium hydrogen phosphate, 0.2% sodium chloride, and 96.95% water. Experimental group 5: soy peptone 1.5%, corn starch 1.5%, calcium carbonate 0.05%, dipotassium hydrogen phosphate 0.1%, sodium chloride 0.2%, water 96.65%. Inoculate 1 strain of Bacillus thuringiensis spores in the above medium and culture normally for 42 hours until the crystals are completely released (such as Figure 1 ) to determine the bioavailability of the fermentation broth. The test found that the bioavailability of the fermentation broth from Experimental Group 2 was 3679.9 IU / mL, higher than that of the other experimental groups (see Table 3). Therefore, the formulation of Experimental Group 2 was the preferred formulation for Bacillus thuringiensis fermentation.

[0054] Table 3 shows the toxicity test results of different formulations on diamondback moth larvae

[0055]

[0056] Example 2: Preparation of Bacillus thuringiensis liquid

[0057] A Bacillus thuringiensis liquid was produced using the formula in Experimental Group 2. Each component was added to a fermenter in proportion and sterilized at 121°C for 30 minutes. After cooling to 34°C, 1000 mL of Bacillus thuringiensis spore liquid was added. The culture conditions were controlled as follows: a speed of 220 rpm / min, a temperature of 34°C, and a ventilation of 3 m 3 / min, the tank pressure is maintained at 0.3-0.4MPa. The fermentation culture is continued for about 42h until more than 90% of the crystals are released and the pH value is about 8.0. The fermentation product is passed through a 200-mesh vibration sieve and collected for later use, which is the Bacillus thuringiensis liquid ( Figure 2 ).

[0058] Example 3: Preparation of Suyun•Rice Granular Suspension Concentrate

[0059] The Bacillus thuringiensis liquid was tested for bioactivity, with a titer of 3697.1 IU / mL (Table 3). The liquid was concentrated 5-10 times using a disc centrifuge, and the concentrate was collected. The titer was ≥16,000 IU / mL.

[0060] Rice leaf folders were reared in the laboratory and inoculated with rice leaf folder granulovirus. Dead insect carcasses were collected and virus preparations were made. The suspension of rice leaf folder granulovirus was prepared at 100 million / ml ( Figure 3 ).

[0061] Mix 16,000 IU / ml Bacillus thuringiensis concentrate and 100 million IU / ml rice leaf roller granule virus suspension at a ratio of 1,000:1, add 0.2%~0.4% sodium benzoate to the mixture, adjust the pH to 4.0~5.0, add 0.1%~0.3% silicone, and finally complete the preparation of Bacillus thuringiensis rice granule suspension.

[0062] Table 4 Determination of the biological activity of fermented Bacillus thuringiensis liquid against Plutella xylostella

[0063]

[0064] Example 4: Analysis of the characteristics of UAV spraying of Suyun·Daozong granular suspension

[0065] The prepared Suyun Rice Vertical Granular Suspension was diluted 10-fold for drone spraying. The test was conducted using the common flight control parameters of the T20 DJI drone: flight altitude 1.5-2 m, nozzle flow rate 6 L / min, and spray width 4 m. The prepared Suyun Rice Vertical Granular Suspension was able to completely pass through the drone filter and settle evenly at the top, middle, and bottom of the color-developing paper, making it suitable for drone spraying. Figure 4a 、 Figure 4b .

[0066] Example 5: Evaluation of the effect of drone application of Suyun·Daozong granules wettable powder and Suyun·Daozong granules suspension concentrate

[0067] 500g of Suyun·Daozong granules wettable powder and 500ml of Suyun·Daozong granules suspension were added to 10L of water and added to the drone medicine box for spraying experiments. After 10s of continuous spraying of Suyun·Daozong granules wettable powder, the filter was completely blocked ( Figure 5 Right), the final passing rate of the medicine is only 7%, while Suyun Rice Granules Suspension Concentrate can be sprayed continuously until the medicine is used up ( Figure 5 Left), the drug pass rate is 100%.

[0068] Example 6: Effect of Suyun Rice Granule Suspension Concentrate on Rice Leaf Folder

[0069] During the peak egg hatching period of the rice leaf roller, 100 ml of Suyun Rice Granule suspension was applied per mu using a drone sprayer. Seven and 14 days after application, the occurrence of rice leaf rollers in the control and control areas was investigated. The results showed that after 7 days of application, the adjusted mortality rate of rice leaf rollers reached 89%. After 14 days, the adjusted mortality rate was still 77%, indicating that it can effectively control the damage caused by rice leaf rollers. Figure 6 At the same time, the number of natural enemies in the field was investigated 7 days after application. Compared with the control, the number of natural enemies in the fields treated with Suyun Rice Granules was basically the same, and significantly more than that in the fields treated with chemical pesticides. This shows that Suyun Rice Granules is more specific than chemical pesticides and is safer against non-target insects, making it an ideal biological pesticide for controlling rice leaf folders.

[0070] The above-mentioned embodiments are only for illustrating the technical features and concepts of the present invention. Their purpose is to enable technicians familiar with this technology in the field to understand the content of the present invention and implement it. It cannot be used to limit the scope of protection of the present invention. Any equivalent changes or modifications made according to the spirit and implementation of the present invention should be included in the scope of protection of the present invention.

Claims

1. The preparation method of Suyun Rice Granular Suspension Concentrate is characterized by: The thuringia·rice granule suspension comprises a Bacillus thuringiensis concentrate and a rice leaf roller granule virus suspension; The Bacillus thuringiensis concentrate has a Bacillus thuringiensis bioavailability of ≥16,000 IU / ml; The rice leaf roller granule virus suspension has a rice leaf roller granule virus content of ≥100,000 OB / ml; The volume ratio of the Bacillus thuringiensis concentrate to the rice leaf roller granule virus suspension is 1000:(1-2); The Suyun•Rice granular suspension concentrate further comprises an adjuvant, wherein the adjuvant comprises sodium benzoate and organosilicon, and the weight of the sodium benzoate is 0.2-0.4wt% of the weight of the Suyun•Rice granular suspension concentrate; The weight of the organosilicon is 0.1-0.3wt% of the weight of the Suyun•Rice granular suspension concentrate; The preparation method comprises: The fermentation product is obtained by fermenting Bacillus thuringiensis spore liquid using a soluble culture medium; the soluble culture medium comprises: Soy peptone 1wt%-2.5wt%, corn starch 1wt%-1.5wt%, calcium carbonate 0.05wt%-0.1wt%, dipotassium hydrogen phosphate 0.05wt%-0.1wt%, sodium chloride 0.1wt%-0.2wt%, water 95.6%-97.8wt%; The fermented product was passed through a vibrating sieve to collect the Bacillus thuringiensis liquid; The Bacillus thuringiensis liquid is concentrated by centrifugation to obtain a Bacillus thuringiensis concentrated liquid; Add the formulated amount of rice leaf roller granule virus to the Bacillus thuringiensis concentrate and mix evenly. Add adjuvants and adjust the pH value to 4.0-6.0 to obtain the Bacillus thuringiensis rice granule suspension concentrate.

2. The method for preparing the Suyun•Rice granular suspension concentrate according to claim 1, characterized in that: The method of fermenting the Bacillus thuringiensis spore liquid with a soluble culture medium to obtain a fermentation product comprises: The soluble culture medium was added to the fermentation tank, sterilized at 121°C for 30 min, and then cooled to 34°C, and then the Bacillus thuringiensis spore liquid was added for fermentation. The fermentation control culture conditions were: rotation speed 180-250 rpm / min, temperature 30-34°C, ventilation 3 m 3 / min, the fermentation tank pressure is maintained at 0.3-0.4MPa; The fermentation culture is continued for 35-45 hours until more than 90% of the crystals are released and the pH value is 8.0 to obtain a fermentation product.

3. The method for preparing the Suyun•Rice granular suspension concentrate according to claim 1, characterized in that: The mesh of the vibration screen is 200 meshes.

4. The method for preparing the Suyun•Rice granular suspension concentrate according to claim 1, characterized in that: The Bacillus thuringiensis liquid is centrifuged and concentrated by 5-10 times using a disc centrifuge, and the concentrated liquid is collected, wherein the Bacillus thuringiensis biological titer in the concentrated liquid is ≥16000 IU / ml; the rotation speed of the disc centrifuge is 4000-6000 rpm / min.

5. The method for preparing the Suyun•Rice granular suspension concentrate according to claim 1, characterized in that: The Bacillus thuringiensis concentrate is added with a formulated amount of rice leaf roller granule virus, an adjuvant is added, and the pH value is adjusted to 4.0-6.0, comprising: The Bacillus thuringiensis concentrate and the rice leaf roller granule virus suspension are evenly mixed, 0.2% to 0.4 wt% of sodium benzoate is added to the mixture, the pH value is adjusted to 4.0 to 5.0 with sulfuric acid, and 0.1% to 0.3 wt% of organosilicon is added.

6. The application method of the Suyun•Rice granular suspension concentrate obtained by the preparation method according to any one of claims 1 to 5, characterized in that: The application method is: during the peak hatching period of rice leaf roller eggs, dilute the Suyun Rice Granule Suspension Concentrate 10 times, use 100-150 ml of the Suyun Rice Granule Suspension Concentrate per acre of rice field, and spray evenly according to the standard parameters of drone flight control.

7. The application method of the Suyun•Rice Granular Suspension Concentrate according to claim 6, characterized in that: The standard parameters for drone flight control are a flight altitude of 1.5 to 2 meters, a nozzle flow rate of 6L / min, and a spray width of 4 meters.

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