Composite soil remediation material as well as preparation method and application thereof

Abstract

The invention relates to the technical field of soil remediation, and particularly discloses a composite soil remediation material as well as a preparation method and application thereof. The composite soil remediation material is prepared from the following raw material components in parts by mass: 15 to 25 parts of Schwertmannite loaded with microbial bacterial liquid, 5 to 10 parts of a biological surfactant, 10 to 20 parts of edible fungus residues, 20 to 30 parts of natural mineral aggregate, 1 to 5 parts of bicarbonate and 70 to 80 parts of water, wherein the microbial bacterial liquid comprises arthrobacter globiformis, serratia rubra and pichia anomala. By means of the technical scheme, the problems that in the prior art, saline-alkali soil remediation is high in remediation cost, long in time, harsh in application condition, prone to secondary pollution and poor in remediation effect are effectively solved.

Description

Technical Field

[0001] The invention relates to the technical field of soil remediation, and specifically discloses a composite soil remediation material and a preparation method and application thereof. Background Art

[0002] Saline-alkali land refers to the type of land that contains excessive soluble salts (such as sodium chloride, sodium sulfate, sodium carbonate, etc.) and alkaline substances (such as sodium carbonate, sodium bicarbonate, etc.) in the soil, which leads to the deterioration of the physical and chemical properties of the soil and affects the normal growth and development of plants. The existence of saline-alkali land not only causes a waste of land resources, but also has many negative impacts on the local ecological environment, agricultural production and economic development, such as low vegetation coverage, decreased land productivity, increased soil erosion, and reduced biodiversity. Therefore, conducting research and practice on saline-alkali land soil remediation has important practical significance and strategic value.

[0003] In the prior art, the remediation of saline-alkali soil includes physical remediation, chemical remediation and biological remediation. Physical remediation methods include deep plowing, irrigation and drainage engineering, and soil restoration, but the labor cost is high and the effect is minimal; chemical remediation methods mainly use chemical drugs to repair saline-alkali soil, such as gypsum, ferrous sulfate or composite soil remediation agents. Although they have a certain repair effect, they need to be applied multiple times, resulting in high agent costs, and are prone to cause accumulation of heavy metal ions in the soil, which poses potential hazards to the soil ecological environment. Biological remediation methods mainly repair by planting salt-alkali tolerant plants or applying microbial agents. Planting salt-alkali tolerant plants has good ecological and economic benefits and can improve the ecological environment of the soil, but the repair effect is slow and it takes a long time to show the repair effect. Microbial agents have the advantages of high efficiency, environmental protection, and no secondary pollution. They can work synergistically with other remediation measures to improve soil remediation effects and plant growth performance. However, their effects are greatly affected by soil environmental conditions (such as temperature, humidity, salinity, pH value, etc.), and the survival and reproduction of microbial agents require suitable substrates and living space. In actual applications, it is necessary to rationally select and optimize the use methods based on soil conditions and plant needs.

[0004] Therefore, developing a soil remediation material with low cost and good remediation effect has important practical significance for the remediation of saline-alkali soil. Summary of the invention

[0005] In view of the problems of high cost, long time, harsh application conditions, easy secondary pollution and poor repair effect in the prior art of saline-alkali soil remediation, the present invention provides a composite soil remediation material and its preparation method and application. The composite soil remediation material includes Schmidt mineral loaded with microbial liquid, biosurfactant, edible fungus residue, natural mineral material, bicarbonate and water; wherein the microbial liquid includes Arthrobacter sphaeroides, Serratia rubrum and Pichia anomala. The synergistic effect of each component is used to give the composite soil remediation material an efficient repair ability; more importantly, the composite soil remediation material is easy to apply and not prone to secondary pollution, providing a new idea for the remediation of saline-alkali soil.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: The first aspect of the present invention provides a composite soil remediation material, comprising the following raw material components in parts by weight: 15-25 parts of Schmidt mineral loaded with microbial liquid, 5-10 parts of biological surfactant, 10-20 parts of edible fungus residue, 20-30 parts of natural mineral material, 1-5 parts of bicarbonate and 70-80 parts of water; wherein the microbial liquid comprises Arthrobacter sphaeroides, Serratia rubrum and Pichia anomala.

[0007] The present invention loads the microbial liquid on the surface of the Schreiber mineral, and utilizes the good adsorption performance and large specific surface area of ​​the Schreiber mineral to place the microorganisms loaded on its surface in a relatively stable environment, provide physical protection for the microorganisms, and avoid interference with the microorganisms by complex soil changes. In addition, the Schreiber mineral can absorb and store influencing elements such as phosphorus, nitrogen and iron from the soil as a nutrient supply for the microorganisms, which helps the microbial liquid maintain metabolic activity for a long time.

[0008] The microbial liquid can repair saline-alkali soil well, wherein the cells of spherical Arthrobacter have a certain osmotic pressure regulation mechanism. In a high-salt environment, spherical Arthrobacter can absorb salt in the soil into the cell, reduce the salt content in the soil, and then use its own metabolic activity to mutually promote the absorbed salt ions, thereby reducing the alkalinity of the soil. The cells of Serratia crimsonensis avoid carrying an electric charge, and can adsorb harmful salt ions such as sodium ions in the soil through ion exchange, fix the salt ions on the cell surface, and reduce their concentration in the soil. In addition, Serratia crimsonensis can improve the soil structure, increase the porosity of the soil, and discharge salt from the soil with water flow; acidic substances will be produced during its metabolism, which can neutralize the alkalinity of the soil and reduce the pH value of the soil. Abnormal Pichia yeast will produce organic acids during its growth and metabolism, which can neutralize the alkaline components in the soil and reduce the degree of salinization of the soil. In addition, Pichia anomalosa can activate soil salt, decompose organic matter in the soil, release the nutrients needed for plant growth, secrete plant growth hormones, stimulate the growth and development of plant roots, and improve the plant's tolerance to saline-alkali environments.

[0009] The addition of biosurfactants can reduce the binding force between soil particles and salt ions, making salt easier to dissolve and wash out, preventing soil compaction caused by salt accumulation, reducing the tight accumulation of soil, and helping to improve the metabolic growth of microbial liquids and the repair performance of composite soil repair materials. More importantly, biosurfactants can improve the efficiency of plants in absorbing water and nutrients, help plants reduce saline-alkali stress, and improve the adaptability of plants to saline-alkali environments. Edible mushroom residues can provide a large amount of nutrients for plant growth, supplement soil fertility, and promote plant growth in saline-alkali soils. In addition, mushroom residues provide a rich carbon source for soil microorganisms, which can promote the growth and reproduction of soil microorganisms, help improve soil properties and structure, and improve soil texture.

[0010] Natural minerals have a large specific surface area and rich surface charge. They can absorb salt ions in the soil through ion exchange and reduce the salt concentration in the soil. Natural minerals can also support loose soil, help improve the air permeability and water permeability of the soil, and facilitate the leaching of salt. Bicarbonate hydrolyzes in the soil environment to produce carbon dioxide, which escapes from the soil pores, helping to loosen the soil, increase the porosity of the soil, and improve the air permeability and water permeability of the soil.

[0011] In summary, the present invention utilizes the synergistic effect of each component raw material to provide a composite soil remediation material with high efficiency in repairing saline-alkali soil. The composite soil remediation material can effectively solve the problems of high remediation cost, long time, harsh application conditions, easy secondary pollution and poor remediation effect in the prior art of saline-alkali soil remediation, and provides a new design idea for the remediation of saline-alkali soil.

[0012] Preferably, the ratio of the number of live bacteria of Arthrobacter sphaeroides, Serratia rubra and Pichia anomala in the microbial liquid is 1:1:0.8-1:1:1, and the concentration of live bacteria in the microbial liquid is (1×10 9 )CFU / mL-(1×10 10 )CFU / mL.

[0013] Preferably, the deposit number of the Arthrobacter sphaeroides is BNCC139139.

[0014] Preferably, the deposit number of the Serratia rubrum is BNCC364420.

[0015] Preferably, the deposit number of the anomalous Pichia pastoris is BNCC186318.

[0016] Preferably or further preferably, the method for preparing the Schmidt mineral loaded with microbial liquid comprises the following steps: S1, filtering the acid mine wastewater, adding an oxidant to the filtrate, carrying out an oxidation reaction, adjusting the pH to 2-4, filtering, and obtaining a brown-yellow solid; acid-washing the brown-yellow solid 3-5 times, drying, and grinding to obtain Schmidtite; S2, mixing the cultured Arthrobacter sphaeroides, the cultured Serratia rubrum and the cultured Pichia anomala according to the designed ratio to obtain a microbial culture solution; S3, dispersing the Schreiber mineral in a buffer solution, adjusting the pH to 5-7, slowly adding the microbial solution, mixing evenly, stirring at a speed of 100rpm-200rpm for 6h-8h, separating the solid and the liquid, and drying naturally to obtain the Schreiber mineral loaded with the microbial solution.

[0017] Further preferably, in S1, the oxidant is a hydrogen peroxide solution with a mass fraction of 20%-35%.

[0018] Further preferably, in S1, the mass ratio of the oxidant to the filtrate is 1:4-1:6.

[0019] Further preferably, in S1, the temperature of the oxidation reaction is 80°C-90°C, and the time of the oxidation reaction is 8h-10h.

[0020] Further preferably, in S1, a strong base is used to adjust the pH to 2-4.

[0021] Further preferably, in S1, the pickling uses a sulfuric acid solution with a concentration of 0.5 mol / L-1 mol / L as a washing liquid.

[0022] Further preferably, in S1, the drying temperature is 85°C-100°C, and the drying time is 6h-10h.

[0023] Further preferably, in S3, the mass volume ratio of the Schreiber mineral to the buffer solution is 1 g:3 mL-1 g:5 mL.

[0024] Further preferably, in S3, the buffer is a phosphate buffer.

[0025] Further preferably, in S3, the mass volume ratio of the Schreiber mineral to the microbial liquid is 1 g:1 mL-1 g:2 mL.

[0026] Preferably, the biosurfactant comprises rhamnolipid and trehalolipid in a mass ratio of 1:0.1-1:0.2.

[0027] Preferably, the edible fungus residue includes any one or more of Pleurotus eryngii residue, Pleurotus ostreatus residue or Lentinus edodes residue.

[0028] Preferably, the natural mineral materials are zeolite and fly ash in a mass ratio of 1:2-1:3.

[0029] Further preferably, the particle size of the natural mineral material is 100 μm-200 μm.

[0030] The second aspect of the present invention provides a method for preparing the composite soil remediation material, comprising the following steps: According to the designed ratio, Schmidt mineral loaded with microbial liquid, edible fungus residue, and natural mineral material are weighed and mixed evenly to obtain a solid mixture; according to the designed ratio, the remaining components are weighed and mixed evenly to obtain a liquid mixture; the solid mixture and the liquid mixture are mixed evenly to obtain a composite soil remediation material.

[0031] The third aspect of the present invention provides application of the composite soil remediation material in the field of saline-alkali soil remediation.

[0032] Preferably, the release amount of the composite soil remediation material is 30kg / mu-35kg / mu.

[0033] Preferably, the water content of the saline-alkali soil is 65%-85%.

[0034] In summary, the present invention provides a composite soil remediation material and its preparation method and application, which utilizes the synergistic effect of each component raw material to enable the composite soil remediation material to have the ability to efficiently repair saline-alkali soil; and the application conditions of the composite soil remediation material are simple, the remediation cost is low, and secondary pollution is not easy to occur. The use of the composite soil remediation material can effectively solve the problems of high remediation cost, long time, harsh application conditions, easy secondary pollution and poor remediation effect in the prior art of saline-alkali soil remediation, and provides a new idea for the remediation of saline-alkali soil. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] The specific preparation methods of the microbial liquid used in each embodiment and comparative example are as follows: Arthrobacter sphericalus was inoculated into a nutrient broth medium and activated and cultured at 28°C for 30 hours to obtain an activated strain; a single colony after activation was picked and inoculated into a seed culture medium, and secondary seed culture was carried out at 28°C and an oscillation speed of 150 rpm for 30 hours to obtain a seed solution; the seed solution was inoculated into a fermentation medium at a volume ratio of 8%, and fermented and cultured at 28°C until the number of viable bacteria reached 1×10 9 CFU / mL.

[0037] The formula of the nutrient broth culture medium is: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 15g of agar and 1000mL of water; the pH of the nutrient broth culture medium is 7.1.

[0038] The formula of the seed culture medium is: 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride, and 1000 mL of water, and the pH of the seed culture medium is 7.1.

[0039] The formula of the fermentation medium is: 20 g of glucose, 10 g of yeast extract, 3 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate, 5 g of sodium chloride and 1000 mL of water, and the pH of the fermentation medium is 7.1.

[0040] The Serratia rubrum was inoculated on LB solid culture medium and activated at 30°C for 24 hours to obtain activated strains; the activated single colony was picked and inoculated into liquid seed culture medium, and secondary seed culture was carried out at 30°C and an oscillation speed of 180 rpm for 20 hours to obtain seed liquid; the seed liquid was inoculated into the fermentation medium at an inoculum amount of 8% of the fermentation medium volume, and fermented at 30°C and an oscillation speed of 180 rpm until the number of viable bacteria reached 1×10 9 CFU / mL.

[0041] The formula of the LB solid culture medium is: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 15 g of agar and 1000 mL of water; the pH of the nutrient broth culture medium is 7.3.

[0042] The formula of the liquid seed culture medium is: 20 g of peptone, 10 g of sodium chloride, 10 g of yeast extract powder and 1000 mL of water; the pH value of the liquid seed culture medium is 7.3.

[0043] The formula of the fermentation medium is: 20 g of glucose, 10 g of yeast extract, 3 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate, 5 g of sodium chloride and 1000 mL of water, and the pH of the fermentation medium is 7.1.

[0044] The abnormal Pichia yeast was inoculated on the activation medium, and the activation culture was carried out at 30°C for 36 hours to obtain an activated bacterial solution; under sterile conditions, the activated bacterial solution was inoculated into the seed medium at an inoculation rate of 3% of the volume ratio of the seed medium, and secondary seed culture was carried out at 30°C and an oscillation speed of 150 rpm for 20 hours to obtain a seed solution; the seed solution was inoculated into the fermentation medium at an inoculation rate of 8% of the volume ratio of the fermentation medium, and fermented and cultured at 28°C, a stirring speed of 200 rpm, and a ventilation volume of 1.5 vvm until the number of viable bacteria reached 1×10 9 CFU / mL.

[0045] The formula of the activation culture medium is: 20g of peptone, 20g of glucose, 10g of yeast powder and 1000mL of water; the pH of the activation culture medium is 6.0.

[0046] The formula of the seed culture medium is: 20 g of peptone, 20 g of glucose, 10 g of yeast powder and 1000 mL of water; the pH of the seed culture medium is 6.0.

[0047] The formula of the fermentation medium is: 30 g of phosphoric acid, 1.5 g of calcium sulfate, 30 g of potassium chloride, 3.5 g of potassium hydroxide, 100 g of glucose, 40 g of magnesium sulfate heptahydrate, 2.0 g of ammonium sulfate, 1.5 g of casein, 4.0 g of tryptone and 1000 mL of water, and the pH of the fermentation medium is 6.5.

[0048] Example 1 The present embodiment provides a composite soil remediation material and a preparation method thereof, which includes the following components by weight: 20 parts of Schweiner mineral loaded with microbial liquid, 8 parts of biosurfactant, 15 parts of mushroom residue, 25 parts of natural mineral material, 3 parts of sodium bicarbonate and 75 parts of water.

[0049] The biosurfactant is rhamnolipid and trehalose lipid in a mass ratio of 1:0.1; the natural mineral material is a mixture of zeolite and fly ash with a particle size of 150 μm, and the mass ratio of zeolite to fly ash is 1:2.5.

[0050] According to the designed ratio, Schweiner minerals loaded with microbial liquid, mushroom residues, and natural mineral materials are weighed and mixed evenly to obtain a solid mixture; according to the designed ratio, the remaining components are weighed and mixed evenly to obtain a liquid mixture; the solid mixture and the liquid mixture are mixed evenly to obtain a composite soil remediation material.

[0051] The method for preparing the Schmidt mineral loaded with microbial liquid comprises the following steps: S1. Filter 1L of acidic mine wastewater, add 200mL of 25% hydrogen peroxide solution to the filtrate, carry out oxidation reaction at 85°C for 8h, adjust the pH to 3.1 with sodium hydroxide, let stand for precipitation, filter, and obtain a brown-yellow solid; acid-wash the brown-yellow solid 4 times with a 0.8mol / L sulfuric acid solution, dry at 90°C for 8h, and grind to obtain Schmidtite; S2, mixing the cultured Arthrobacter sphaeroides, the cultured Serratia rubrum and the cultured Pichia anomala in a mass ratio of 1:1:0.9 to obtain a microbial culture solution; S3, dispersing 200g of the Schmidt mineral in 1L of phosphate buffer, adjusting the pH to 6.2, slowly adding the microbial culture solution, mixing evenly, stirring at a speed of 150rpm for 7h, centrifuging, discarding the liquid, and drying naturally to obtain the Schmidt mineral loaded with the microbial culture solution.

[0052] Example 2 The present embodiment provides a composite soil remediation material and a preparation method thereof, which includes the following components by weight: 25 parts of Schweiner mineral loaded with microbial liquid, 10 parts of biological surfactant, 20 parts of Pleurotus ostreatus residue, 30 parts of natural mineral material, 5 parts of sodium bicarbonate and 80 parts of water.

[0053] The biosurfactant is rhamnolipid and trehalose lipid in a mass ratio of 1:0.2; the natural mineral material is a mixture of zeolite and fly ash with a particle size of 200 μm, and the mass ratio of zeolite to fly ash is 1:2.

[0054] According to the designed ratio, Schweiner minerals loaded with microbial liquid, mushroom residues, and natural mineral materials are weighed and mixed evenly to obtain a solid mixture; according to the designed ratio, the remaining components are weighed and mixed evenly to obtain a liquid mixture; the solid mixture and the liquid mixture are mixed evenly to obtain a composite soil remediation material.

[0055] The method for preparing the Schmidt mineral loaded with microbial liquid comprises the following steps: S1. Filter 1L of acidic mine wastewater, add 200mL of 25% hydrogen peroxide solution to the filtrate, carry out oxidation reaction at 85°C for 8h, adjust the pH to 3.1 with sodium hydroxide, let stand for precipitation, filter, and obtain a brown-yellow solid; acid-wash the brown-yellow solid 4 times with a 0.8mol / L sulfuric acid solution, dry at 90°C for 8h, and grind to obtain Schmidtite; S2, mixing the cultured Arthrobacter sphaeroides, the cultured Serratia rubrum and the cultured Pichia anomala in a mass ratio of 1:1:0.9 to obtain a microbial culture solution; S3, dispersing 200g of the Schmidt mineral in 1L of phosphate buffer, adjusting the pH to 6.2, slowly adding the microbial culture solution, mixing evenly, stirring at a speed of 150rpm for 7h, centrifuging, discarding the liquid, and drying naturally to obtain the Schmidt mineral loaded with the microbial culture solution.

[0056] Example 3 The present embodiment provides a composite soil remediation material and a preparation method thereof, which includes the following components by weight: 15 parts of Schweiner mineral loaded with microbial liquid, 5 parts of biosurfactant, 10 parts of mushroom residue, 20 parts of natural mineral material, 2 parts of sodium bicarbonate and 70 parts of water.

[0057] The biosurfactant is rhamnolipid and trehalose lipid in a mass ratio of 1:0.1; the natural mineral material is a mixture of zeolite and fly ash with a particle size of 100 μm, and the mass ratio of zeolite to fly ash is 1:3.

[0058] According to the designed ratio, Schweiner minerals loaded with microbial liquid, mushroom residues, and natural mineral materials are weighed and mixed evenly to obtain a solid mixture; according to the designed ratio, the remaining components are weighed and mixed evenly to obtain a liquid mixture; the solid mixture and the liquid mixture are mixed evenly to obtain a composite soil remediation material.

[0059] The method for preparing the Schmidt mineral loaded with microbial liquid comprises the following steps: S1. Filter 1L of acidic mine wastewater, add 200mL of 25% hydrogen peroxide solution to the filtrate, carry out oxidation reaction at 85°C for 8h, adjust the pH to 3.1 with sodium hydroxide, let stand for precipitation, filter, and obtain a brown-yellow solid; acid-wash the brown-yellow solid 4 times with a 0.8mol / L sulfuric acid solution, dry at 90°C for 8h, and grind to obtain Schmidtite; S2, mixing the cultured Arthrobacter sphaeroides, the cultured Serratia rubrum and the cultured Pichia anomala in a mass ratio of 1:1:0.9 to obtain a microbial culture solution; S3, dispersing 200g of the Schmidt mineral in 1L of phosphate buffer, adjusting the pH to 6.2, slowly adding the microbial culture solution, mixing evenly, stirring at a speed of 150rpm for 7h, centrifuging, discarding the liquid, and drying naturally to obtain the Schmidt mineral loaded with the microbial culture solution.

[0060] Comparative Example 1 This comparative example provides a composite soil remediation material and a preparation method thereof, which is different from Example 1 in that Arthrobacter sphaeroides in the microbial culture solution is replaced with an equal amount of Burkholderia, and other components and processes remain unchanged, which will not be described in detail here.

[0061] The preparation method of the Burkholderia bacterial liquid comprises the following contents: The Burkholderia was inoculated on a nutrient broth medium and activated at 28°C for 30 hours to obtain an activated strain; the activated single colony was picked and inoculated into a seed culture medium, and secondary seed culture was carried out at 28°C and an oscillation speed of 150 rpm for 30 hours to obtain a seed solution; the seed solution was inoculated into a fermentation medium at a volume ratio of 8%, and fermented at 28°C until the number of viable bacteria reached 1×10 9 CFU / mL.

[0062] The formula of the nutrient broth culture medium is: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 15g of agar and 1000mL of water; the pH of the nutrient broth culture medium is 7.1.

[0063] The formula of the seed culture medium is: 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride, and 1000 mL of water, and the pH of the seed culture medium is 7.1.

[0064] The formula of the fermentation medium is: 20 g of glucose, 10 g of yeast extract, 3 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate, 5 g of sodium chloride and 1000 mL of water, and the pH of the fermentation medium is 7.1.

[0065] Comparative Example 2 This comparative example provides a composite soil remediation material and a preparation method thereof, which is different from Example 1 in that the Serratia rubrum in the microbial culture liquid is replaced by an equal amount of Bacillus megaterium, and other components and processes remain unchanged, which will not be described in detail here.

[0066] The preparation method of the Bacillus megaterium liquid comprises the following contents: Bacillus megaterium was inoculated into a nutrient broth medium and activated at 28°C for 30 hours to obtain an activated strain; a single colony after activation was picked and inoculated into a seed medium, and secondary seed culture was performed at 28°C and an oscillation speed of 150 rpm for 30 hours to obtain a seed solution; the seed solution was inoculated into a fermentation medium at a volume ratio of 8%, and fermented at 28°C until the number of viable bacteria reached 1×10 9 CFU / mL.

[0067] The formula of the nutrient broth culture medium is: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 15g of agar and 1000mL of water; the pH of the nutrient broth culture medium is 7.1.

[0068] The formula of the seed culture medium is: 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride, and 1000 mL of water, and the pH of the seed culture medium is 7.1.

[0069] The formula of the fermentation medium is: 20 g of glucose, 10 g of yeast extract, 3 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate, 5 g of sodium chloride and 1000 mL of water, and the pH of the fermentation medium is 7.1.

[0070] Comparative Example 3 This comparative example provides a composite soil remediation material and a preparation method thereof. The difference from Example 1 is that the Schreiner mineral loaded with microbial liquid is replaced by an equal amount of Schreiner mineral, and the other components and preparation methods remain unchanged, which will not be repeated here.

[0071] Performance Testing The composite soil repair material obtained in each embodiment and comparative example is used in saline-alkali soil. The soil is rotary tilled and then irrigated and drained. When the soil moisture content is 75%-80%, the composite soil repair material is applied at 30 kg per mu. After 10 days of repair, peanuts are planted in the test field, and the local conventional method is adopted to prepare the land and plant peanuts. The peanut variety is Luhua No. 9. All treatments are prepared at the same time, and sowing is carried out at the same time. After sowing, local conventional field management is adopted. After the peanuts are harvested, the peanut yields of different treatments are counted, and the salt content, pH value, organic matter content, and soil particle size percentage at 0-20 cm of the soil are measured. The results are shown in Table 1.

[0072] Table 1 Comparison of organic matter content before and after restoration of composite soil restoration materials obtained in various examples and comparative examples

[0073] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composite soil remediation material, characterized in that: The invention comprises the following raw material components in parts by weight: 15-25 parts of Schmidt mineral loaded with microbial liquid, 5-10 parts of biological surfactant, 10-20 parts of edible fungus residue, 20-30 parts of natural mineral material, 1-5 parts of bicarbonate and 70-80 parts of water; wherein the microbial liquid comprises Arthrobacter sphaeroides, Serratia rubrum and Pichia anomala.

2. The composite soil remediation material according to claim 1, characterized in that: The ratio of the number of live bacteria of Arthrobacter sphaeroides, Serratia rubra and Pichia anomala in the microbial liquid is 1:1:0.8-1:1:1, and the concentration of live bacteria in the microbial liquid is (1×10 8 )CFU / mL-(1×10 9 )CFU / mL.

3. The composite soil remediation material according to claim 1 or 2, characterized in that: The preparation method of the Schmidt mineral loaded with microbial liquid comprises the following steps: S1, filtering the acid mine wastewater, adding an oxidant to the filtrate, carrying out an oxidation reaction, adjusting the pH to 2-4, filtering, and obtaining a brown-yellow solid; acid-washing the brown-yellow solid 3-5 times, drying, and grinding to obtain Schmidtite; S2, mixing the cultured Arthrobacter sphaeroides, the cultured Serratia rubrum and the cultured Pichia anomala according to the designed ratio to obtain a microbial culture solution; S3, dispersing the Schreiber mineral in a buffer solution, adjusting the pH to 5-6, slowly adding the microbial solution, mixing evenly, stirring at a speed of 100-200 rpm for 6-8 hours, separating the solid and the liquid, and drying naturally to obtain the Schreiber mineral loaded with the microbial solution.

4. The composite soil remediation material according to claim 3, characterized in that: In S1, the oxidant is a hydrogen peroxide solution with a mass fraction of 20%-35%; and / or In S1, the mass ratio of the oxidant to the filtrate is 1:4-1:6; and / or In S1, the temperature of the oxidation reaction is 80°C-90°C, and the time of the oxidation reaction is 8h-10h.

5. The composite soil remediation material according to claim 3, characterized in that: In S3, the mass volume ratio of the Schroeder mineral to the buffer solution is 1 g:3 mL-1 g:5 mL; and / or In S3, the mass volume ratio of the Schreiber mineral to the microbial liquid is 1 g:1 mL-1 g:2 mL.

6. The composite soil remediation material according to claim 1, characterized in that: The biosurfactant comprises rhamnolipid and trehalose lipid in a mass ratio of 1:0.1-1:0.2; and / or The edible fungus residue includes any one or more of Pleurotus eryngii residue, Pleurotus ostreatus residue or Lentinus edodes residue; and / or The natural mineral materials are zeolite and fly ash in a mass ratio of 1:2-1:

3.

7. The method for preparing the composite soil remediation material according to any one of claims 1 to 6, characterized in that: The steps include: According to the designed ratio, Schmidt mineral loaded with microbial liquid, edible fungus residue, and natural mineral material are weighed and mixed evenly to obtain a solid mixture; according to the designed ratio, the remaining components are weighed and mixed evenly to obtain a liquid mixture; the solid mixture and the liquid mixture are mixed evenly to obtain a composite soil remediation material.

8. Use of the composite soil remediation material according to any one of claims 1 to 6 in the field of saline-alkali soil remediation.

9. The use of the composite soil remediation material according to claim 8 in the field of saline-alkali soil remediation, characterized in that: The release amount of the composite soil remediation material is 30kg / mu-35kg / mu; and / or The water content of the saline-alkali soil is 65%-85%.

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