An external composition, preparation and application thereof with soothing and anti-inflammatory effects

Abstract

The present invention belongs to the technical field of cosmetics and provides an external composition, a preparation and their applications with soothing and anti-inflammatory effects. The external composition is composed of, by weight: 4-5 parts of Viola philippica Cav. extract, 4-5 parts of Matricaria chamomilla L. extract, 1-3 parts of Taraxacum officinale Weber var. officinale Dahlst. rhizome / root extract, 1-3 parts of Paeonia lactiflora Pall. root extract, 1-3 parts of Lonicera japonica Thunb. flower extract, 1-3 parts of Malva sylvestris L. flower extract, and 0.001-0.1 part of Aloe barbadensis Miller leaf extract; wherein, the Taraxacum officinale Weber var. officinale Dahlst. rhizome / root extract is prepared by the following steps: pulverizing the Taraxacum officinale Weber var. officinale Dahlst. rhizome / root, extracting with an ethanol-aqueous solution, concentrating the extract, enzymolyzing with a composite enzyme preparation, and purifying with a macroporous adsorption resin column to obtain the extract. The content and yield of the total flavonoids, the effective active ingredient in the Taraxacum officinale Weber var. officinale Dahlst. rhizome / root extract in the external composition of the present invention are high. The composition of the present invention has the characteristics of a small dosage and good soothing and anti-inflammatory effects on allergic skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional health care products, and relates to an external composition, a preparation and their applications with soothing and anti-inflammatory effects. Background Art

[0002] As the largest organ of the human body, the skin can prevent the loss of substances such as water and electrolytes in the body, and prevent the invasion of harmful substances from the outside, maintaining the stability of the internal environment of the human body. However, with the widespread use of daily chemical products, more and more women have skin sensitivity problems. Coupled with the influence of lifestyle, the probability of various allergens that induce immune allergic reactions, such as dust mites, pollen, pet hair, food additives, etc., is increasing. As a result, the incidence of allergic skin problems such as atopic dermatitis / eczema, allergic dermatitis, and sensitive skin has increased, attracting the attention of all parties.

[0003] At present, western medicine drugs mainly adopt anti-allergy treatment, without a radical cure effect, are prone to recurrence and contain hormone components more or less, with side effects. Nowadays, people are more in pursuit of health and naturalness, and some natural active ingredients are very popular, such as Chinese herbal medicines, plants, etc. For example, Chinese Patent Application CN102883732A discloses a composition containing artemisia annua extract and amine compounds and a method for treating skin with the composition, and Chinese Patent CN105213321B discloses the effects of artemisia annua, artemisinin or artemisinin derivatives in atopic dermatitis / eczema, allergic asthma, allergic rhinitis, allergic dermatitis, sensitive skin, etc. However, they all contain monomeric compounds, resulting in increased costs and the need for further improvement in effects. Chinese Patent Application CN114732770A discloses a composition applied to soothing anti-skin allergy cosmetics, which contains chamomile, common dandelion, aloe vera, common mallow, peony root, honeysuckle, and viola yedoensis. However, this application uses the original plants as medicine, with a large dosage, and at the same time, it does not disclose the effect data, and its soothing and anti-inflammatory effects need to be further improved.

[0004] Therefore, there is an urgent need to provide an external composition derived from plants with a small dosage and good soothing and anti-inflammatory effects on allergic skin. Summary of the Invention

[0005] In view of the problems existing in the prior art, the present invention provides an external composition, a preparation and their applications with soothing and anti-inflammatory effects. By improving the preparation method of dandelion rhizome / root leaf extract, the content and yield of the total flavonoids, the effective components, are effectively increased. The composition composed of the obtained extract and other components has the characteristics of a small dosage and good soothing and anti-inflammatory effects on allergic skin.

[0006] The present invention is realized through the following technical solutions:

[0007] The first aspect of the present invention is to provide an external composition with soothing and anti-inflammatory effects. The external composition is composed of the following components by weight: 4-5 parts of Viola philippica Cav. extract, 4-5 parts of Matricaria chamomilla L. extract, 1-3 parts of Taraxacum officinale F.H. Wigg. rhizome / root extract, 1-3 parts of Paeonia lactiflora Pall. root extract, 1-3 parts of Lonicera japonica Thunb. flower extract, 1-3 parts of Malva sylvestris L. flower extract, and 0.001-0.1 part of Aloe barbadensis Miller leaf extract;

[0008] Among them, the Taraxacum officinale F.H. Wigg. rhizome / root extract is prepared by the following steps:

[0009] (1) Extraction: The original medicinal material, Taraxacum officinale F.H. Wigg. rhizome / root, is pulverized and added to an ethanol-aqueous solution for extraction to obtain a Taraxacum officinale F.H. Wigg. rhizome / root extract solution;

[0010] (2) Enzymolysis: The Taraxacum officinale F.H. Wigg. rhizome / root extract solution is concentrated to remove ethanol, diluted with water, placed in an enzymolysis tank, and a compound enzyme preparation is added for enzymolysis to obtain an enzymolysis solution;

[0011] (3) Separation and purification: The enzymolysis solution is loaded onto a macroporous adsorption resin column and eluted with a water-ethanol system. The eluate is concentrated and dried to obtain the Taraxacum officinale F.H. Wigg. rhizome / root leaf extract.

[0012] Preferably, in step (1), the concentration of the ethanol-aqueous solution is 60-80% (V / V); the mass-volume ratio of Taraxacum officinale F.H. Wigg. rhizome / root to the ethanol-aqueous solution is 1:6-10 (g:mL).

[0013] Preferably, in step (1), the extraction temperature is 45-65 °C, and extraction is carried out 2-3 times, each time for 20-30 min.

[0014] Preferably, in step (2), the compound enzyme preparation includes 1-2 parts of cellulase, 0.05-0.2 part of pectinase, and 0.1-0.5 part of β-glucosidase; the dosage of the compound enzyme preparation is 1-3 wt.% of Taraxacum officinale F.H. Wigg. rhizome / root;

[0015] The enzymolysis temperature is 35-40 °C, and the enzymolysis time is 60-80 min.

[0016] Preferably, in step (3), the filler of the macroporous adsorption resin column is a styrene-based macroporous adsorption resin, specifically selected from any one of HPD417, ADS-17, DM130, or HP-20.

[0017] Preferably, in step (3), the volume ratio of the enzymolysis solution to the filler of the macroporous adsorption resin column is 4-9:1, and the elution is first with water washing and then with gradient elution using water-ethanol solutions of different concentrations.

[0018] Further preferably, the elution volume of water in the water washing is 3 - 5 BV, the flow rate is 1 - 2 BV / h, preferably 2 BV / h.

[0019] Further preferably, the volume ratios of the water - ethanol solutions with different concentrations used in the gradient elution are 90:10 - 10:90, the total volume of the eluent used is 4 - 12 BV, the flow rate is 1 - 2 BV / h, preferably 1.5 BV / h, and the eluent in the part of 40:60 - 20:80 is collected.

[0020] Even more preferably, the content of total flavonoids in the rhizome / root and leaf extract of Taraxacum officinale is ≥50%.

[0021] The second aspect of the present invention lies in providing a preparation comprising the above - mentioned external composition.

[0022] Preferably, the preparation further comprises pharmaceutically acceptable excipients.

[0023] Preferably, the dosage form of the preparation is an emulsion, a spray, an aerosol, a cream, an aqueous solution, a gel, an oil, a patch, a film or a mud - type agent.

[0024] The third aspect of the present invention lies in the application of the above - mentioned external composition or the preparation comprising the above - mentioned external composition in the preparation of a cosmetic with soothing and anti - inflammatory effects.

[0025] Compared with the prior art, the present invention has the following beneficial effects: (1) By optimizing the enzymatic hydrolysis process of the rhizome / root of Taraxacum officinale (including the selection of enzyme preparations and the regulation of dosage) and purifying with a macroporous adsorption resin column, the content and yield of the effective active ingredient total flavonoids in the rhizome / root of Taraxacum officinale are greatly improved; (2) The composition of the present invention has the characteristics of a small dosage and good soothing and anti - inflammatory effects on allergic skin. Detailed implementation manners

[0026] It should be noted that the raw materials used in the present invention without specific limitation on their sources are all ordinary commercially available products.

[0027] Example 1:

[0028] The rhizome / root extract of Taraxacum officinale is prepared by the following steps:

[0029] (1) Take the rhizome / root of Taraxacum officinale, crush it, add it to an ethanol - aqueous solution (75% (V / V)), and extract it twice at 50°C. For the first extraction, the solid - liquid ratio (the rhizome / root powder of Taraxacum officinale to the ethanol - aqueous solution) is 1:8 (g / mL), extract for 30 min, filter, and for the second extraction of the filter residue, the solid - liquid ratio is 1:6 (g / mL), extract for 20 min, and combine the filtrates to obtain the rhizome / root extract of Taraxacum officinale;

[0030] (2) Concentrate the extract of Taraxacum officinale rhizome / root, remove ethanol, dilute with water, place it in an enzymolysis tank, add a compound enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio 1:0.1:0.25) accounting for 2 wt% of the amount of Taraxacum officinale rhizome / root, and carry out enzymolysis at 35 °C for 60 min to obtain an enzymolysate;

[0031] (3) Centrifuge the enzymolysate at 50000 rpm for 5 min, take the supernatant, pass it through a macroporous adsorption resin column (HPD417), the volume ratio of the enzymolysate to the macroporous adsorption resin column packing is 5:1, first elute with 4 BV of water at a flow rate of 2 BV / h, then elute with a water-ethanol solution with a volume ratio of 90:10 - 10:90, the total volume of the eluent used is 6 BV, the flow rate is 1.5 BV / h, collect the eluent in the part of 40:60 - 20:80, concentrate and dry to obtain the said Taraxacum officinale rhizome / root extract.

[0032] Example 2:

[0033] The Taraxacum officinale rhizome / root extract is prepared by the following steps:

[0034] (1) Take the Taraxacum officinale rhizome / root, crush it, add it to an ethanol-aqueous solution (75% (V / V)), extract it twice at 50 °C. For the first extraction, the material-liquid ratio (Taraxacum officinale rhizome / root powder to ethanol-aqueous solution) is 1:8 (g / mL), extract for 30 min, filter, and use the filter residue for the second extraction with a material-liquid ratio of 1:6 (g / mL), extract for 20 min, combine the filtrates to obtain the Taraxacum officinale rhizome / root extract;

[0035] (2) Concentrate the extract of Taraxacum officinale rhizome / root, remove ethanol, dilute with water, place it in an enzymolysis tank, add a compound enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio 2:0.05:0.5) accounting for 1 wt% of the amount of Taraxacum officinale rhizome / root, and carry out enzymolysis at 35 °C for 60 min to obtain an enzymolysate;

[0036] (3) Centrifuge the enzymolysate at 50000 rpm for 5 min, take the supernatant, pass it through a macroporous adsorption resin column (ADS-17), the volume ratio of the enzymolysate to the macroporous adsorption resin column packing is 5:1, first elute with 4 BV of water at a flow rate of 2 BV / h, then elute with a water-ethanol solution with a volume ratio of 90:10 - 10:90, the total volume of the eluent used is 6 BV, the flow rate is 1.5 BV / h, collect the eluent in the part of 40:60 - 20:80, concentrate and dry to obtain the said Taraxacum officinale rhizome / root extract.

[0037] Example 3:

[0038] The Taraxacum officinale rhizome / root extract is prepared by the following steps:

[0039] (1) Crush the roots / rhizomes of *Taraxacum officinale*, add them to an ethanol-aqueous solution (75% (V / V)), and extract twice at 50 °C. For the first extraction, the ratio of the roots / rhizomes of *Taraxacum officinale* powder to the ethanol-aqueous solution is 1:8 (g / mL), extract for 30 min, filter, and use the filter residue for the second extraction with a ratio of 1:6 (g / mL) and extract for 20 min. Combine the filtrates to obtain the extraction solution of the roots / rhizomes of *Taraxacum officinale*;

[0040] (2) Concentrate the extraction solution of the roots / rhizomes of *Taraxacum officinale*, remove ethanol, dilute with water, place it in an enzymatic hydrolysis tank, add a compound enzyme preparation (the mass ratio of cellulase, pectinase, and β-glucosidase is 1:0.2:0.1) accounting for 3 wt% of the amount of the roots / rhizomes of *Taraxacum officinale*, and perform enzymatic hydrolysis at 35 °C for 60 min to obtain an enzymatic hydrolysate;

[0041] (3) Centrifuge the enzymatic hydrolysate at 50000 rpm for 5 min, take the supernatant, pass it through a macroporous adsorption resin column (DM130), with the volume ratio of the enzymatic hydrolysate to the packing volume of the macroporous adsorption resin column being 5:1. First, elute with 4 BV of water at a flow rate of 2 BV / h, then elute with a water-ethanol solution with a volume ratio of 90:10 - 10:90. The total volume of the eluent used is 6 BV, and the flow rate is 1.5 BV / h. Collect the eluent in the range of 40:60 - 20:80, concentrate and dry it to obtain the extract of the roots / rhizomes of *Taraxacum officinale*.

[0042] Comparative Example 1:

[0043] The difference from Example 1 is only that: the compound enzyme preparation is replaced with cellulase and pectinase (mass ratio 1:0.1), and the total dosage is the same as the total dosage of the compound enzyme preparation in Example 1.

[0044] Comparative Example 2:

[0045] The difference from Example 1 is only that: the compound enzyme preparation is replaced with a single β-glucosidase, and the dosage is the same as the total dosage of the compound enzyme preparation in Example 1.

[0046] Comparative Example 3:

[0047] The extract of the roots / rhizomes of *Taraxacum officinale* is prepared by the following steps:

[0048] (1) Crush the roots / rhizomes of *Taraxacum officinale*, add them to an ethanol-aqueous solution (75% (V / V)), and extract twice at 50 °C. For the first extraction, the ratio of the roots / rhizomes of *Taraxacum officinale* powder to the ethanol-aqueous solution is 1:8 (g / mL), extract for 30 min, filter, and use the filter residue for the second extraction with a ratio of 1:6 (g / mL) and extract for 20 min. Combine the filtrates to obtain the extraction solution of the roots / rhizomes of *Taraxacum officinale*;

[0049] (2)Concentrate the extract of Taraxacum officinale rhizome / root, remove ethanol, dilute with water, place it in an enzymatic hydrolysis tank, add a compound enzyme preparation (cellulase, pectinase, β-glucosidase mass ratio 1:0.1:0.25) accounting for 2 wt% of the amount of Taraxacum officinale rhizome / root, and carry out enzymatic hydrolysis at 35 °C for 60 min to obtain an enzymatic hydrolysate;

[0050] (3)Concentrate and dry the enzymatic hydrolysate to obtain the Taraxacum officinale rhizome / root extract.

[0051] The difference from Example 1 is that the enzymatic hydrolysate in step (3) is directly concentrated and dried without purification by a macroporous resin column.

[0052] Test Example 1:

[0053] According to the method in the literature "Liu Jia. Research on the alleviating effect of total flavonoids from Taraxacum mongolicum Hand.-Mazz. on heat stress in Caenorhabditis elegans [D]. Fuyang Normal University, 2019.", the total flavonoid content in the Taraxacum officinale rhizome / root extracts prepared in the above examples and comparative examples was determined, and the amount of total flavonoids in the Taraxacum officinale rhizome / root was measured. The yield of total flavonoids was calculated based on the amount of total flavonoids in the Taraxacum officinale rhizome / root extract, and the results are shown in Table 1.

[0054] Table 1: Content and yield results of total flavonoids in the Taraxacum officinale rhizome / root extract of the present invention

[0055]

[0056] Comparing Comparative Example 1 and Comparative Example 2 with Example 1, it can be seen that: in the present invention, cellulase and pectinase, as a whole, cooperate with β-glucosidase, which can significantly increase the content and yield of total flavonoids in the Taraxacum officinale rhizome / root extract.

[0057] Comparing Comparative Example 3 with Example 1, it can be seen that: the macroporous resin chromatography column has a good separation and purification effect on flavonoids in the enzymatic hydrolysate, increasing the content of total flavonoids in the Taraxacum officinale rhizome / root extract. On the premise of equal total flavonoid amount, the dosage of the Taraxacum officinale rhizome / root extract obtained in the present invention is smaller.

[0058] Test Example 2:

[0059] 1. Medicine: The Taraxacum officinale rhizome / root extracts obtained in Examples 1-3 and Comparative Examples 1-3 are combined with other components of the present invention to form the compositions of Examples 1-3 and Comparative Examples 1-3. Composition of the composition: 4 parts of Viola philippica Cav. extract, 4 parts of Matricaria recutita L. extract, 3 parts of Taraxacum officinale rhizome / root extract (Examples 1-3, Comparative Examples 1-3), 2 parts of Paeonia lactiflora Pall. root extract, 1 part of Lonicera japonica Thunb. flower extract, 1 part of Althaea officinalis L. flower extract, and 0.1 part of Aloe barbadensis Miller leaf extract.

[0060] 2. Seeding Hacat cells

[0061] Digesting cells: Irradiate with ultraviolet light for 20 - 30 min, spray the wet tissue with alcohol for disinfection, and wipe the laminar flow hood. Take out the cell culture flask, disinfect it and put it into the laminar flow hood, pour out the culture medium, wash twice with PBS buffer, pour out the PBS, add 1 mL of TE buffer, after sufficient contact, pour out a small amount of TE, digest in the incubator for 8 - 9 min, and observe under the microscope.

[0062] Terminating digestion: When the cells are completely digested from the culture flask wall, add 5 mL of complete culture medium to terminate digestion, and resuspend the cell suspension with a disposable pipette.

[0063] Cell counting: Count the original cell solution. Take 10 μL to one side of the counting plate and 10 μL to the other side, count 2 - 3 times, take the average value, calculate the cell density, and calculate and prepare the cell solution according to the required cell density and volume for seeding.

[0064] Seeding: Add 200 μL of cell solution to each well of the 96 - well plate, with a cell density of 2.0×10 5 cells / mL, and place it in the incubator at 37 °C in a 5% CO2 environment for 8 - 12 h to allow the cells to adhere and grow.

[0065] 3. Loading cells

[0066] (1)Washing the plate: Take out the 96 - well plate, suck out the culture medium with a pipette gun, and wash twice with 200 μL of PBS.

[0067] (2)Loading samples: Set up blank group, model group and drug groups. The blank group is added with 200 μL of serum - free culture medium; the model group is added with 200 μL of serum - free culture medium; the drug groups are added with 200 μL of different samples (dilute the compositions of Examples 1 - 3 and Comparative Examples 1 - 3 with serum - free culture medium respectively). After 2 h, add bacteria for stimulation (cell:bacteria = 1:20), and continue to culture for 8 h.

[0068] 4. Detection with TNF - α ELISA kit

[0069] The collected cell culture supernatant was detected according to the instructions of the ELISA kit, and the specific results are shown in Table 2.

[0070] Table 2: Experimental results of TNF - α content detection in each group

[0071]

[0072] Compared with the blank group, the model group ## indicates P < 0.01; compared with the model group, * in the drug groups indicates P < 0.05, ** indicates P < 0.01; compared with the composition of Example 1, in the drug groups & indicates P < 0.05,&& Indicates P < 0.01.

[0073] Compared with the blank group, the content of TNF-α in Hacat cells was significantly up-regulated after stimulation with Propionibacterium acnes, indicating successful modeling. Compared with the model group, after adding the composition samples of Examples 1-3 and Comparative Examples 1-3, the content of TNF-α in the cells decreased significantly. Among them, the compositions of Examples 1-3 showed significant inhibition of TNF-α secretion, and the inhibition of TNF-α secretion by the compositions of Comparative Examples 1-3 was statistically significant. Compared with the composition of Example 1, the inhibitory effect of the compositions of Comparative Examples 1-3 on TNF-α secretion was lower than that of the composition of Example 1. Among them, the effects of the compositions of Comparative Examples 1 and 2 were significantly different from that of the composition of Example 1, and the effect of the composition of Comparative Example 3 was statistically significantly different from that of the composition of Example 1. Therefore, through the optimization of the enzymolysis process of Taraxacum officinale rhizome / root (including the selection of enzyme preparations and the regulation of dosage) and purification by macroporous adsorption resin column, the composition composed of Taraxacum officinale rhizome / root extract and other components has the best inhibitory effect on TNF-α secretion and shows good anti-inflammatory effect.

[0074] Test Example 3:

[0075] The composition samples of Examples 1-3 and Comparative Examples 1-3 in Test Example 2 were respectively prepared into 10% aqueous solutions with deionized water as the test sample solutions.

[0076] In this experiment, SD rats were selected and randomly divided into a blank group, a model group, Example 1-3 groups, and Comparative Example 1-3 groups, with 10 rats in each group. They were depilated 24 hours before the experiment, and the hair on the back of the animals was removed, with an area of about 3 cm × 3 cm. Except for the blank group, the other groups were modeled on the back skin of rats with 2,4-dinitrochlorobenzene (DNCB) and irradiated with ultraviolet light for 12 hours. After successful modeling (the skin showed symptoms such as dryness, desquamation, burning, stinging, swelling, and erythema). The blank group and the model group were respectively coated with 0.5 mL of deionized water on the depilated skin area of the back, with a coating range of about 2.5 cm × 2.5 cm. The other groups were respectively coated with 0.5 mL of the test sample solutions of different groups once a day for 21 consecutive days. On the 22nd day, the skin conditions of the right back of the rats in each group were observed with the naked eye, recorded according to the test evaluation criteria in Table 3, and the concentrations of histamine (HIS) and immunoglobulin E (IgE) in the serum of each sample were detected.

[0077] Table 3: Test evaluation criteria for skin reactions

[0078]

[0079] The skin reactions of the rats in each group and the concentrations of HIS and IgE in the serum are shown in Table 4.

[0080] Table 4: Skin reactions of rats in each group and concentrations of HIS and IgE in serum

[0081]

[0082] Compared with the blank group, the model group ## Indicates P < 0.01; compared with the model group, * in the drug group indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001; compared with the composition of Example 1, the drug group & Indicates P < 0.05, && Indicates P < 0.01.

[0083] The allergic symptoms, and the concentrations of HIS and IgE in serum of the rats in the model group were significantly higher than those in the blank group, with very significant statistical significance; after applying the sample solutions of Examples 1 - 3 and Comparative Examples 1 - 3 of the present invention, the concentrations of HIS and IgE were significantly lower than those in the model group, and the allergic symptoms were significantly alleviated and almost all returned to normal levels, indicating that the composition of the present invention has obvious anti - inflammatory and anti - irritation effects and can well soothe allergic skin and symptoms such as skin dryness, desquamation, swelling, and erythema caused by ultraviolet irradiation; at the same time, in terms of reducing the concentrations of HIS and IgE and alleviating allergic symptoms, the compositions of Comparative Examples 1 - 3 had significantly lower effects than the compositions of Examples 1 - 3.

[0084] In summary, through the optimization of the enzymolysis process of Taraxacum officinale roots / rhizomes (including the selection of enzyme preparations and the regulation of dosage) and the purification by macroporous adsorption resin columns, the composition composed of the extract of Taraxacum officinale roots / rhizomes and other components has obvious anti - inflammatory and soothing effects.

[0085] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than a limitation on the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art does not depart from the essence and scope of the technical solution of the present invention.

Claims

1. An external composition with soothing and anti-inflammatory effects, characterized in that, The external composition is composed of the following components by weight: 4-5 parts of Viola philippica Cav. extract, 4-5 parts of Matricaria recutita L. extract, 1-3 parts of Taraxacum officinale Weber var. officinale root / rhizome extract, 1-3 parts of Paeonia lactiflora Pall. root extract, 1-3 parts of Lonicera japonica Thunb. flower extract, 1-3 parts of Malva sylvestris L. flower extract, and 0.001-0.1 part of Aloe barbadensis Miller leaf extract; The Taraxacum officinale Weber var. officinale root / rhizome extract is prepared by the following steps: (1) Extraction: After pulverizing the original medicinal material of Taraxacum officinale Weber var. officinale root / rhizome, adding an ethanol-aqueous solution for extraction, and filtering the solution to obtain the Taraxacum officinale Weber var. officinale root / rhizome extract solution; (2) Enzymolysis: Concentrating the Taraxacum officinale Weber var. officinale root / rhizome extract solution, removing ethanol, diluting with water, placing it in an enzymolysis tank, and adding a compound enzyme preparation for enzymolysis to obtain an enzymolysis solution; (3) Separation and purification: Loading the enzymolysis solution onto a macroporous adsorption resin column, eluting with a mobile phase, concentrating and drying the eluate to obtain the Taraxacum officinale Weber var. officinale root / rhizome extract; Wherein In step (1), the volume concentration of the ethanol-aqueous solution is 60-80%, the mass-volume ratio of Taraxacum officinale Weber var. officinale root / rhizome to the ethanol-aqueous solution is 1 g: 6-10 mL, the extraction temperature is 45-65 °C, extracting 2-3 times, 20-30 min each time; In step (2), the compound enzyme preparation is composed of 1-2 parts of cellulase, 0.05-0.2 part of pectinase, and 0.1-0.5 part of β-glucosidase by weight, the dosage of the compound enzyme preparation is 1-3 wt% of Taraxacum officinale Weber var. officinale root / rhizome, the enzymolysis temperature is 35-40 °C, and the enzymolysis time is 60-80 min; In step (3), the filler of the macroporous adsorption resin column is a styrene-based macroporous adsorption resin, specifically selected from any one of HPD417, ADS-17, DM130, or HP-20.

2. The external composition according to claim 1, wherein In step (3), the volume ratio of the enzymolysis solution to the filler of the macroporous adsorption resin column is 4-9:1, the elution is first washing with water, and then gradient eluting with water-ethanol solutions of different concentrations, concentrating and drying the eluate.

3. The external composition according to claim 2, characterized in that, The volume ratio of the water-ethanol solutions of different concentrations used for the gradient elution is 90:10-10:90, the total volume of the eluate used is 4-12 BV, and the eluate in the part of 40:60-20:80 is collected.

4. The external composition according to claim 3, characterized in that, The content of total flavonoids in the Taraxacum officinale Weber var. officinale root / rhizome extract is ≥50%.

5. A preparation containing the external composition according to any one of claims 1-4, characterized in that, The dosage form of the preparation is one or more of an emulsion, a spray, an aerosol, a cream, an aqueous solution, a gel, an oil, a patch, a film, or a mud preparation.

6. Use of the external composition according to any one of claims 1-4 or the preparation according to claim 5 in the preparation of a cosmetic having a soothing and anti-inflammatory effect.

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