Artificial cultivation method of sphagnum moss and application of artificial cultivation method

Abstract

The invention belongs to the technical field of sphagnum moss planting, and particularly relates to an artificial cultivation method of sphagnum moss and application of the artificial cultivation method. The artificial cultivation method of the sphagnum moss comprises the following steps: placing a sphagnum moss stem segment growing in season in a culture container containing a nutrient solution for illumination culture to obtain artificially cultivated sphagnum moss; the nutrient solution comprises ammonium sulfate, calcium superphosphate and calcium chloride; the illumination intensity of the illumination culture is 230 to 6000 lux. The artificial cultivation method for the sphagnum moss has the advantages that the sphagnum moss is promoted to grow, the growth vigor of the sphagnum moss is good, and a direction is provided for industrial artificial cultivation of the sphagnum moss.

Description

Technical Field

[0001] The invention belongs to the technical field of peat moss planting, and particularly relates to an artificial cultivation method of peat moss and application thereof. Background Art

[0002] Sphagnumpalustre, also known as sphagnum moss or seaweed in my country, is a type of bryophyte with unique morphological, physiological, biochemical and developmental characteristics. It is an "ecosystem engineer" of peatlands, has unique and irreplaceable ecological value, and plays an important role in maintaining ecosystems and regulating global climate. Because peat moss has super water absorption capacity and certain antibacterial and anti-inflammatory effects, whether it is dry or decomposed, it has been widely used in the horticultural industry and has become the most economically valuable type of bryophyte. In addition, peat moss has an adsorption and accumulation effect on a variety of atmospheric pollutants, metals and metalloids. Peat moss has a high research value in both basic research and applied research.

[0003] However, most of the widely used peat mosses come from the wild. Excessive exploitation of peat mosses will damage the wetland ecological environment and aggravate the greenhouse effect. Artificial cultivation and planting of peat mosses can effectively solve this problem. At present, artificially planted peat mosses grow slowly and poorly. There is a lack of a method for quickly propagating peat moss in the prior art. Summary of the invention

[0004] In order to solve the problem of slow efficiency and poor growth of artificial propagation of peat moss, the present invention provides an artificial cultivation method capable of rapidly propagating peat moss, which specifically includes the following technical solutions:

[0005] An artificial cultivation method of peat moss comprises the following steps:

[0006] Placing the stem segments of peat moss grown in the current season in a culture container containing a nutrient solution for light culture to obtain artificially cultivated peat moss;

[0007] The nutrient solution comprises ammonium sulfate, superphosphate and calcium chloride; the illumination intensity of the light culture is 230-6000 lux.

[0008] Preferably, the length of the sphagnum moss stem segment grown in the current season is 0.5 to 1 cm.

[0009] Preferably, the placement density of the sphagnum moss stem segments grown in the current season is 1 plant / 2 cm 2 .

[0010] Preferably, the step of placing the sphagnum moss stem segments grown in the current season in a culture container containing a nutrient solution further comprises the step of placing filter paper in the culture container;

[0011] The step of placing the filter paper comprises the following operations: trimming two sheets of filter paper to the same size as the bottom surface of the culture container, overlapping and laying them flat in the culture container.

[0012] Preferably, after the filter papers are overlapped and laid flat in the culture container, the humidity of the filter papers is maintained at 100%.

[0013] Preferably, in the nutrient solution, the concentration of ammonium sulfate is 0.1-100 mg / L, the concentration of superphosphate is 0.1-100 mg / L, and the concentration of potassium chloride is 0.1-100 mg / L; the ratio of the amount of the nutrient solution added to the dry weight of the peat moss is 20-25 mL: 1 g.

[0014] Preferably, the temperature of the light culture is 15-20°C, the light cycle is L / D=12h / 12h, and the time is 28 days.

[0015] Preferably, the culture process also includes the step of replenishing nutrient solution every 7 days.

[0016] Preferably, when the nutrient solution is supplemented, the ratio of the amount of the nutrient solution supplemented each time to the dry weight of the peat moss is 20-25 mL: 1 g.

[0017] The invention also provides application of the artificial cultivation method in artificial propagation of peat moss.

[0018] The beneficial effects of the present invention are:

[0019] The present invention provides an artificial cultivation method for peat moss, comprising the steps of selecting stem segments of peat moss growing in the current season, dividing them into small segments and then culturing them, and using a nutrient solution for culturing. Cultivating peat moss using the cultivation method of the present invention can accelerate the cultivation speed of peat moss and improve the cultivation quality of peat moss. Compared with untreated peat moss plants, the length, area, number of new shoots, number of regenerated gametophytes, length of gametophytes, and number of gametophytes of the treated plants are significantly increased. The artificial cultivation method for peat moss involved in the present invention provides theoretical support for the large-scale production and application of peat moss, and is of great significance to the protection of wild peat moss and the industrial development of peat moss. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0021] Figure 1 This is a schematic diagram of differentiating sphagnum moss stem segments according to nodes in Example 2 of the present invention;

[0022] Figure 2 It is a schematic diagram of the growth results of different stem segments after culturing peat moss in Example 2 of the present invention;

[0023] Figure 3 This is a schematic diagram of the number of gametophytes after culturing sphagnum moss in different stem segments in Example 2 of the present invention;

[0024] Figure 4 This is a schematic diagram of the growth results of sphagnum moss after being cultured at different light intensities in Example 3 of the present invention;

[0025] Figure 5 The statistical results of the gametophyte length after culturing peat moss at different light intensities in Example 3 of the present invention;

[0026] Figure 6 The statistical results of the number of gametophytes after culturing peat moss at different light intensities in Example 3 of the present invention;

[0027] Figure 7 The statistical results of the length of the growing gametophytes after culturing peat moss in different nutrient solutions in Example 4 of the present invention;

[0028] Figure 8 The statistical results of the growth area of ​​gametophytes after culturing peat moss in different nutrient solutions in Example 4 of the present invention;

[0029] Fig. 9 The statistical results of the number of new shoots after culturing peat moss with different nutrient solutions in Example 4 of the present invention;

[0030] Fig.10 This is a schematic diagram of the growth results of peat moss cultured in different nutrient solutions according to Example 4 of the present invention. DETAILED DESCRIPTION

[0031] The present invention provides an artificial cultivation method of peat moss, comprising the following steps:

[0032] The peat moss stem segments grown in the current season are placed in a culture container containing a nutrient solution for light culture to obtain artificially cultivated peat moss; the nutrient solution comprises ammonium sulfate, superphosphate and calcium chloride; and the light intensity of the light culture is 230-6000 lux.

[0033] As an embodiment, the present invention picks wild peat moss in good growth condition, removes impurities on the peat moss, removes soil and impurities attached to the peat moss, and then obtains peat moss stem segments grown in the current season according to the peat moss section.

[0034] As an embodiment, the present invention places filter paper in a culture container to obtain a culture container covered with filter paper; as an embodiment, the step of placing the filter paper includes the following operations: trimming two filter papers to the same size as the bottom of the culture container, overlapping and laying them flat in the culture container to obtain a culture container covered with filter paper.

[0035] After obtaining the culture container covered with filter paper, as an embodiment, the present invention places a nutrient solution in the culture container covered with filter paper to obtain a culture container containing nutrient solution. As an embodiment, in the nutrient solution, the concentration of ammonium sulfate is 0.1-100 mg / L, the concentration of superphosphate is 0.1-100 mg / L, and the concentration of potassium chloride is 0.1-100 mg / L; as an embodiment, in the nutrient solution, the concentration ranges of ammonium sulfate, superphosphate and potassium chloride can include but are not limited to 0.1-10 mg / L, 0.1-50 mg / L, 10-100 mg / L, 50-100 mg / L, respectively; as an embodiment, in the nutrient solution, the concentrations of ammonium sulfate, superphosphate and potassium chloride can be any one of 0.1 mg / L, 10 mg / L, 50 mg / L and 100 mg / L, respectively. As an embodiment, the nutrient solution is added in an amount of 20 to 25 mL / g relative to the dry weight of peat moss; as an embodiment, the ratio of the added amount of the nutrient solution to the peat moss can be any one of 20 mL / g, 21 mL / g, 22 mL / g, 23 mL / g, 24 mL / g and 25 mL / g.

[0036] After obtaining the seasonally grown sphagnum moss stem segments and the culture container containing the nutrient solution, the present invention places the seasonally grown sphagnum moss stem segments in the culture container containing the nutrient solution for light culture; using the seasonally grown sphagnum moss stem segments for culture can accelerate the rate of artificial cultivation of sphagnum moss and increase the number of sphagnum moss gametophytes produced. As an embodiment, the length of the sphagnum moss stem segments is 0.5 to 1 cm; as an embodiment, the length of the sphagnum moss stem segments can be any one of 0.5 cm, 0.6 cm, 0.7 cm, 0.8 cm, 0.9 cm and 1.0 cm. As an embodiment, the placement density of the seasonally grown sphagnum moss stem segments is 1 plant / 2 cm 2; As an embodiment, the light intensity range includes but is not limited to 230-1500 Lux, 230-5000 Lux, 5000-6000 Lux, 1500-6000 Lux, 1500-5000 Lux. Cultivating peat moss with light of this intensity can promote the growth of peat moss, increase the number and length of peat moss gametophytes, and accelerate the artificial cultivation rate of peat moss; as an embodiment, the light can be any one of 230 Lux, 1500 Lux, 5000 Lux and 6000 Lux. The nutrient solution of the present invention includes ammonium sulfate, superphosphate and calcium chloride; the nutrient solution of the present invention can increase the length and area of ​​the gametophytes generated by peat moss, and can also promote the germination of new shoots of peat moss to increase the yield. As an embodiment, the temperature of the light culture is 15-20 ° C, the photoperiod is L / D=12h / 12h, and the time is 28d. As an embodiment, the temperature of the light culture can be any one of 15°C, 16°C, 17°C, 18°C, 19°C and 20°C. In a specific embodiment, the 0.5 cm long sphagnum moss stem segments grown in the current season of the present invention are 1 plant / 2 cm 2 The ratio of 100 mg / L ammonium sulfate, 100 mg / L superphosphate and 100 mg / L calcium chloride is placed in a culture dish containing a nutrient solution, the nutrient solution includes 100 mg / L ammonium sulfate, 100 mg / L superphosphate and 100 mg / L calcium chloride, the ratio of the added nutrient solution to the dry weight of peat moss is 25 mL / g, and the peat moss is placed in an incubator for light culture, the light intensity of the light culture is 230 Lux, the temperature is 16°C, the photoperiod is L / D=12h / 12h, and the culture time is 28d.

[0037] As an embodiment, the light culture process also includes the step of replenishing nutrient solution every 7 days. As an embodiment, when replenishing the nutrient solution, each time the nutrient solution is replenished, it is added at a ratio of 20 to 25 mL / g to the dry weight of the peat moss, and the humidity of the filter paper is maintained at 100% to maintain the normal growth of the plant. As an embodiment, the amount of the supplementary nutrient solution can be any one of 20 mL / g, 21 mL / g, 22 mL / g, 23 mL / g, 24 mL / g and 25 mL / g.

[0038] In order to further illustrate the present invention, an artificial cultivation method of peat moss and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0039] Embodiment 1 A kind of artificial cultivation method of peat moss

[0040] The artificial cultivation method of peat moss comprises the following steps:

[0041] Select wild peat moss, remove impurities, select the stem segments of peat moss growing in the season, and cut them into small segments of 0.5 cm long;

[0042] After trimming two filter papers to the same size as the bottom of a transparent plastic box, overlap and lay them flat in a transparent plastic box, put the peat moss cut into small pieces into a transparent plastic box of 16cm×14cm×7.5cm, 20 plants in one box, lay them flat, add 50mL of nutrient solution, place the culture container in an incubator, and culture for 28 days at 230lux light, 16℃, L / D=12h / 12h. Add nutrient solution once every 7 days at a ratio of nutrient solution to peat moss dry weight of 25mL:1g, and keep the filter paper moist to obtain artificially cultivated peat moss.

[0043] The nutrient solution comprises: 100 mg / L ammonium sulfate, 100 mg / L superphosphate, and 100 mg / L potassium chloride.

[0044] Example 2 Study on the influence of the selection of the broken stem position of peat moss on the cultivation effect

[0045] Take wild peat moss plants from their native habitat. Wild peat moss has obvious growth seasonality and distinct nodes in different growing seasons. Based on the nodes, the peat moss is divided into distinct upper, middle and lower parts. Figure 1 As shown, the upper part is grown in the current season, the middle part is grown in the previous season, and the lower part is grown in the season before that. This embodiment uses the upper, middle and lower parts of peat moss to conduct experiments respectively.

[0046] The upper, middle and lower parts of the peat moss were evenly placed in a transparent plastic box with a length of 16 cm, a width of 14 cm and a height of 7.5 cm, and 50 mL of water was added. The culture was carried out under the conditions of 8 hours of light, 16 hours of darkness and 5000 lux of light. The number of newly born gametophytes was observed and counted on the 14th, 21st, 28th and 35th days of growth. The results are shown in Table 1 and Figures 2-3 shown.

[0047] Table 1 Number of regenerated gametophytes in different parts of peat moss

[0048]

[0049] According to Table 1 and Figures 2-3 The results show that the upper part of the peat moss can generate more new gametophytes and has a stronger division ability. Both the regeneration speed and the number of regeneration are significantly higher than those of the middle and lower peat moss. This part can be used for cultivation in actual production, and using this part for cultivation can promote the regeneration of peat moss.

[0050] Example 3 Effect of light on peat moss cultivation

[0051] The peat moss with good growth status was collected from the experimental field and brought back to the laboratory for use. Weeds and impurities were removed from the peat moss, and peat moss plants with good growth status (including three parts, upper, middle and lower) were selected. They were divided into stem segments of about 0.5 cm in length with scissors, and placed in a transparent plastic box with a length of 16 cm, a width of 14 cm, and a height of 7.5 cm. About 20 plants were placed in one box. After the boxes were laid flat, 50 mL of tap water was added. 12 boxes of peat moss segments were prepared according to the above method.

[0052] The 12 experimental materials were evenly divided into three groups and placed in incubators of group A, group B and group C respectively. They were cultured under three different light intensities, with the incubator temperature at 16°C and the photoperiod L / D = 16h / 8h. The light intensity of group A was 5000Lux; the light intensity of group B was 1500Lux; and the light intensity of group C was 230Lux.

[0053] The length of gametophytes and the number of gametophyte regeneration in different groups of peat mosses at 2, 3, 4, and 5 weeks of growth were counted. Figure 4 As shown in Tables 2-3 and Figures 5-6 As shown, Figures 5-6 Graphpadprism 8.0 was used as the graphics and data software.

[0054] Table 2 Effect of different light conditions on the length of Sphagnum gametophyte (cm)

[0055]

[0056] Table 3 Effect of different light on the number of peat moss gametophytes (pieces)

[0057]

[0058] From Tables 2 to 3 and Figures 4 to 6 It can be seen that peat moss can grow under 230Lux to 5000Lux light. The light conditions of 230Lux and 1500Lux have a significant promoting effect on the increase of the length of the gametophyte of peat moss, which accelerates the growth of peat moss to a certain extent. There are significant differences in the growth length of the gametophyte of peat moss in different groups. During the peat moss cultivation stage, higher light intensity may inhibit the growth of peat moss.

[0059] Example 4 Effect of nutrient solution components on peat moss growth

[0060] 120 peat moss plants with consistent growth status and good growth, free of impurities, diseases and insect pests were selected, and the top of the peat moss was cut, and the head-shaped branches about 1 cm from the top of the branch were used as the treatment materials. According to the components shown in Table 4, nutrient solutions named CA, CB, and CC were prepared.

[0061] Table 4 Composition of CA nutrient solution, CB nutrient solution and CC nutrient solution

[0062] Element CA CB CC Ammonium sulfate 0.1mg 100mg 1000mg Superphosphate 0.1mg 100mg 1000mg Potassium chloride 0.1mg 100mg 1000mg Make up to volume with distilled water 1000mL 1000mL 1000mL

[0063] The peat moss capitate materials were evenly divided into four groups, 30 plants were treated with CA nutrient solution, 30 plants were treated with CB nutrient solution, 30 plants were treated with CC nutrient solution; 30 plants were treated with clean water as the control group.

[0064] Trim two pieces of filter paper to an appropriate size, overlap and lay them flat on a culture dish with a diameter of 90 mm, place the peat moss heads in the culture dish, treat three dishes in each group, place 10 peat moss heads in each dish, add 5 mL of nutrient solution to each dish, and add 5 mL of water to the control group to maintain the humidity for normal plant growth.

[0065] The culture dish was placed in an incubator for 28 days, the culture temperature was 16°C, the light intensity was 4000-6000 lux, and the photoperiod was 12 hours of light and 12 hours of darkness. During the culture process, 5 mL of aqueous solution was regularly added every 7 days to keep the filter paper moist. After 28 days of culture, the length of the peat moss plants in different groups was recorded. The results are shown in Table 5. Figure 7 As shown; record the plant projection area, the results are shown in Table 6, Figure 8 As shown; record the number of new buds of the plants, the results are shown in Table 7, Fig. 9 As shown; record the growth status of peat moss head branches before and after cultivation, the results are as follows Fig.10 shown.

[0066] Table 5 Plant length after four weeks of growth treated with different concentrations of nutrient solution (cm)

[0067]

[0068]

[0069] Table 6 Plant area (cm2) after four weeks of growth in different concentrations of nutrient solution 2 )

[0070] Number / Concentration Group CK CA CB CC 1 0.582 0.626 1.396 0.353 2 0.608 0.716 1.127 0.264 3 0.419 0.62 0.89 0.27 4 0.601 0.704 0.867 0.272 5 0.455 0.774 0.972 0.211 6 0.44 0.587 1.117 0.201 7 0.629 0.885 1.153 0.403 8 0.44 0.695 1.227 0.388 9 0.598 0.772 0.949 0.332 10 0.656 0.816 0.792 0.411 11 0.617 0.71 0.957 0.381 12 0.592 0.755 0.875 0.329 13 0.629 1.042 0.772 0.245 14 0.517 0.648 0.756 0.343 15 0.416 0.639 0.935 0.426 16 0.404 0.747 1.049 0.383 17 0.359 0.647 1.383 0.294 18 0.668 0.889 0.779 0.345

[0071] Table 7 Number of new shoots after four weeks of growth treated with different concentrations of nutrient solution

[0072]

[0073]

[0074] Figures 7 to 9Graphpad prism 8.0 was used for graphing and data analysis. Data were expressed as Mean ± SEM and analyzed using Student's t-test. Significant differences were indicated by asterisks, where * corresponds to p < 0.05, ** corresponds to p < 0.01, *** corresponds to p < 0.001, and **** corresponds to p < 0.0001.

[0075] From Tables 5 to 7 and Figures 7 to 9 It can be seen that the nutrient solution provided by the present invention can induce the production of a large number of new shoots in a short time, achieve rapid regeneration of peat moss shoots and increase biomass. Compared with the peat moss plants in the control group, the length, area and number of new shoots of the treated plants increased significantly.

[0076] Among them, the differences in plant length, plant orthographic projection area and number of new shoots between the peat moss plants treated with CA nutrient solution and CB nutrient solution and the untreated peat moss plants were extremely significant. The length and area of ​​the peat moss treated with CA nutrient solution were 1.3 times that of the control. The average length of the peat moss materials treated with CA nutrient solution could reach 3.1 cm, and the average area could reach 0.74 cm 2 The length and area of ​​sphagnum moss treated with CB nutrient solution are 1.7 times that of the control. The average length of sphagnum moss treated with CB nutrient solution can reach 3.9cm, and the average area can reach 1cm 2 Compared with the control peat moss, the plant length and plant orthographic projection area of ​​the peat moss treated with CC nutrient solution were inhibited.

[0077] Depend on Fig.10 It can be seen that compared with the control group, the peat moss treated with CA and CB nutrient solutions had significant differences in growth length and growth area, while CC nutrient solution significantly inhibited the growth of peat moss. Therefore, CA nutrient solution and CB nutrient solution treatment can promote the rapid regeneration of peat moss.

[0078] In summary, the artificial cultivation method of peat moss provided by the present invention can increase the growth length and number of peat moss gametophytes, promote the rapid growth of peat moss, and improve the growth quality of peat moss. The present invention provides a feasible, efficient and simple method for artificially cultivating peat moss.

[0079] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments like this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for artificially cultivating peat moss, characterized in that: The steps include: Placing the stem segments of peat moss grown in the current season in a culture container containing a nutrient solution for light culture to obtain artificially cultivated peat moss; The nutrient solution comprises ammonium sulfate, superphosphate and calcium chloride; the illumination intensity of the light culture is 230-6000 lux.

2. The artificial cultivation method according to claim 1, characterized in that: The length of the sphagnum moss stem segment grown in the current season is 0.5 to 1 cm.

3. The artificial cultivation method according to claim 1, characterized in that: The placement density of the sphagnum moss stem segments grown in the current season is 1 plant / 2cm 2 .

4. The artificial cultivation method according to claim 1, characterized in that: The step of placing the sphagnum moss stem segments grown in the current season in front of a culture container containing a nutrient solution also includes the step of placing filter paper in the culture container; The step of placing the filter paper comprises the following operations: trimming two sheets of filter paper to the same size as the bottom surface of the culture container, overlapping and laying them flat in the culture container.

5. The artificial cultivation method according to claim 4, characterized in that: After overlapping and laying the filter papers in the culture container, the humidity of the filter papers was maintained at 100%.

6. The artificial cultivation method according to claim 1, characterized in that: In the nutrient solution, the concentration of ammonium sulfate is 0.1-100 mg / L, the concentration of superphosphate is 0.1-100 mg / L, and the concentration of potassium chloride is 0.1-100 mg / L; the ratio of the added amount of the nutrient solution to the dry weight of the peat moss is 20-25 mL: 1 g.

7. The artificial cultivation method according to claim 1, characterized in that: The temperature of the light culture is 15-20° C., the light cycle is L / D=12h / 12h, and the time is 28 days.

8. The artificial cultivation method according to claim 1, characterized in that: The culture process also includes the step of replenishing nutrient solution every 7 days.

9. The artificial cultivation method according to claim 8, characterized in that: When the nutrient solution is supplemented, the ratio of the amount of the nutrient solution supplemented each time to the dry weight of the peat moss is 20-25 mL: 1 g.

10. Use of the artificial cultivation method according to any one of claims 1 to 9 in artificial propagation of sphagnum moss.

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