A fixation device for mouse brain imaging

Abstract

This invention proposes a novel fixation device for mouse brain imaging, comprising a first fixation component, an adjustment platform connected to the bottom of the first fixation component, a mounting base plate connected to the bottom of the adjustment platform, and a second fixation component mounted on the mounting base plate, with one end of the second fixation component close to the first fixation component. The first fixation component includes an L-shaped plate mounted on the top of the adjustment platform. Two supporting cylinders are symmetrically arranged on the L-shaped plate near the second fixation component. The top of each supporting cylinder is movably connected to a first connecting plate. A brain fixation plate is connected between the two first connecting plates and fixed to the mouse's head. The brain fixation plate has positioning holes for locating the viewing window area for mouse brain imaging. This device reduces stress response in mice during experiments, improves experimental reliability and repeatability, ensures absolute stability of mice in an awake state, and effectively improves the accuracy of experimental data.

Description

Technical Field

[0001] This invention belongs to the field of experimental instrument technology, specifically to a fixation device for mouse brain imaging. Background Technology

[0002] Advances in imaging technology have provided favorable technical conditions for conducting brain science research. Because the structure of the mouse brain is highly similar to that of the human brain, research on the mouse brain can be extended to human brain science research. Previous studies have mostly focused on imaging the brains of mice under anesthesia, with few studies on awake mice. However, imaging the brains of awake mice is of great significance to brain science research, as it can help study the mechanisms of brain function and neural activity, reveal the connections and interactions between different brain regions, and aid in the diagnosis and assessment of diseases. The main obstacles to this research are the small size of the mouse skull and the difficulty in fixing the head and body. Existing fixation devices, such as brackets, clamps, connecting rods, and slide rails, generally use high-precision mechanical components and materials. To ensure tight installation, precision machining is required, resulting in high manufacturing costs. Furthermore, existing mouse fixation devices adjust the operating space through slide rails, spiral adjustment rods, and connecting rods. This limited adjustment space leads to poor adaptability, making it difficult to fix mice of different sizes. The fixation effect is also limited, which in turn makes it impossible to ensure the stability of the mouse's body and brain imaging areas in a conscious state. Consequently, the collected data is affected by motion artifacts, hindering neuroscience research. Summary of the Invention

[0003] To address the problem that existing fixation devices used in experiments cannot stably fix the head of a mouse in a conscious state, this invention provides a fixation device for mouse brain imaging.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] This invention proposes a fixation device for mouse brain imaging, including a first fixation component for fixing the mouse head, an adjustment platform for adjusting the tilt angle and position connected to the bottom of the first fixation component, a mounting base plate connected to the bottom of the adjustment platform, and a second fixation component for fixing the mouse body disposed on one side of the adjustment platform on the mounting base plate, with one end of the second fixation component close to the first fixation component.

[0006] The first fixing component includes a T-shaped plate, which is installed on the top of the adjustment platform. Two supporting cylinders are symmetrically arranged on the T-shaped plate near the second fixing component. A first connecting plate is movably connected to the top of each supporting cylinder. A brain fixing plate is connected between the two first connecting plates. The brain fixing plate is fixed to the mouse head. The brain fixing plate is provided with a positioning hole, which is used to locate the viewing window area for mouse brain imaging.

[0007] Preferably, a second connecting groove is provided on the brain fixation plate near its end, and a first adjusting groove is provided on the first connecting plate at the end position of the brain fixation plate, and the first adjusting groove and the second connecting groove are connected by a connecting screw.

[0008] Preferably, a second adjustment groove is provided on the end of the first connecting plate away from the first adjustment groove, and a first fixing screw is installed in the second adjustment groove, with the bottom end of the first fixing screw fixed to the top of the supporting cylinder.

[0009] Preferably, a third adjustment groove is provided on the end of the L-shaped plate near the second fixing component, and a second fixing screw is connected in the third adjustment groove, with the top end of the second fixing screw fixed to the top of the supporting cylinder.

[0010] Preferably, the adjustment platform includes an installation platform, which is fixedly installed on the installation base plate. A first adjustment table is installed on the top of the installation platform. A first angle adjustment component is provided between the installation platform and the first adjustment table. A first adjustment knob is connected to the first angle adjustment component and is rotatably mounted on the installation platform.

[0011] A third adjustment platform is installed on the top of the first adjustment platform, and a second angle adjustment component is provided between the third adjustment platform and the first adjustment platform. A second adjustment knob is connected to the second angle adjustment component, and the second adjustment knob is rotatably mounted on the first adjustment platform.

[0012] The second adjustment platform is installed on the top of the third adjustment platform. A height adjustment component is provided between the second adjustment platform and the third adjustment platform. A third adjustment knob is connected to the height adjustment component and is installed on the third adjustment platform.

[0013] Preferably, the second fixing component includes a support plate for placing the mouse body. The upper end of the support plate is equipped with multiple sets of fixing components. Each set of fixing components includes two connecting platforms. The mouse body is located between the two connecting platforms in each set. In each set of fixing components, one of the connecting platforms is provided with a groove, and one end of a sliding pressure plate is connected to the other connecting platform. The other end of the sliding pressure plate passes through the groove and is fixed by the fixing rod, so that the sliding pressure plate fixes the mouse body.

[0014] A first adjustment component is connected to the side end face of the support plate opposite to the connecting platform, and the adjustment component is mounted on the mounting base plate.

[0015] Preferably, the support plate is symmetrically provided with sliding grooves, the connecting platform is installed in the sliding grooves, the connecting platform is provided with a fifth fixing hole, the fixing rod is installed in the fifth fixing hole, and one end of the fixing rod is in close contact with the mounting base plate;

[0016] A third fixing member is provided on the support plate near the end of the brain fixation piece. The third fixing member includes a first fixing groove and a second fixing groove. The first fixing groove is used to insert the lower jaw of the mouse after the mouse's mouth is open, and the second fixing groove is used to hold the mouse's incisors.

[0017] Preferably, the first adjustment component includes an L-shaped bracket, which is mounted on the mounting base plate. A connector is connected to one side of the L-shaped bracket, a second connecting plate is connected to the connector, and a third connecting plate is connected to the second connecting plate. The third connecting plate is fixed to the side end face of the support plate.

[0018] Preferably, the mounting base plate is provided with multiple adjustment holes, and the L-shaped bracket is connected to the corresponding adjustment hole by a threaded rod.

[0019] Preferably, a heating pad is provided on the support plate.

[0020] Compared with the prior art, the present invention has the following beneficial technical effects:

[0021] This invention provides a fixation device for mouse brain imaging. The device utilizes a T-shaped plate of the first fixation component to provide a stable support base. The combination of the supporting cylinder and the first connecting plate allows the brain fixation plate to flexibly adapt to different mouse head shapes and sizes, ensuring the stability and accuracy of the mouse head during imaging. Furthermore, the positioning holes on the brain fixation plate precisely locate the imaging window area of ​​the mouse brain, ensuring the imaging window is in the optimal position. This provides significant flexibility for experiments, greatly improving imaging resolution and clarity, and helping researchers more accurately observe and analyze the structure and function of the mouse brain. The adjustable platform facilitates the use of the entire device. The tilt angle and position can be finely adjusted according to experimental needs, which not only helps optimize the imaging angle and improve imaging quality, but also provides researchers with more experimental flexibility, facilitating multi-angle and multi-level brain imaging studies. The second fixation component effectively fixes the mouse's body, avoiding interference caused by the mouse's movement during imaging, ensuring imaging stability, helping to reduce the stress response of the mouse during the experiment, improving the reliability and repeatability of the experiment, ensuring the absolute stability of the mouse in a conscious state, effectively improving the accuracy of experimental data, and reducing the influence of external factors on experimental results, allowing for more complex brain science studies such as brain function and blood flow dynamics.

[0022] Furthermore, the combination of the third adjustment groove and the second fixing screw on the L-shaped plate in this fixing device provides another flexible adjustment method for fixing the supporting cylinder, enhancing the stability of the supporting cylinder and enabling it to better bear the weight of the mouse's head. Moreover, by adjusting the position of the second fixing screw in the third adjustment groove, the height and tilt angle of the supporting cylinder can be further fine-tuned, thereby meeting the precise requirements for fixing the mouse's head under different experimental conditions, which helps to improve the accuracy and reliability of imaging experiments.

[0023] Furthermore, the adjustable platform in this fixation device, which allows for precise adjustment of the mouse's position and angle during experiments by adjusting its tilt angle and position, can adapt to the needs of other experimental equipment, ensuring that specific brain regions are within the observable range, thereby improving experimental efficiency and enabling accurate observation or imaging. Moreover, through reasonable fixation and adjustment, motion artifacts in conscious mice during experiments can be effectively reduced, ensuring the accuracy of images and data acquired in a conscious state. It also helps reduce stress responses in mice, improves their comfort, and contributes to enhancing the reliability of experimental data.

[0024] Furthermore, in this fixation device, the second connecting groove on the brain fixation plate is connected to the first adjusting groove on the first connecting plate via a connecting screw, allowing the brain fixation plate to be flexibly adjusted in position as needed to adapt to different mouse head sizes and shapes. The combination of the second adjusting groove and the first fixing screw on the first connecting plate provides additional fixing points for the brain fixation plate, enhancing the stability and reliability of the fixation, thereby ensuring the absolute stillness of the mouse head during imaging and improving imaging quality.

[0025] Furthermore, the third fixing member provided on the support plate in this fixing device includes a first fixing groove and a second fixing groove, which provide an additional way to fix the mouse's head. The first fixing groove can accommodate the mouse's open jaw, while the second fixing groove is used to hold the mouse's incisors, effectively preventing the mouse's head from moving during the imaging process, further improving the stability and clarity of the imaging, and ensuring the accuracy and reliability of the experimental data.

[0026] Furthermore, this fixation device provides stable support for fixing the mouse body through the combination of an L-shaped bracket, connectors, a second connecting plate, and a third connecting plate, and enables flexible adjustment of the position of the support plate, enhancing the overall stability of the device. This allows researchers to easily adjust the position of the mouse body as needed, thereby optimizing imaging results and improving the accuracy and reliability of the experiment.

[0027] Furthermore, the second fixing component can be flexibly adjusted by multiple adjustment holes on the mounting base plate and the connection between the L-shaped bracket and the adjustment holes via threaded rods. This allows for precise control of the mouse's body fixation position under different experimental requirements, enhancing the versatility and flexibility of the device. It provides researchers with more experimental options and operational convenience, and helps improve the accuracy and efficiency of experiments.

[0028] Furthermore, the fixture is equipped with a heating pad on the support plate to ensure that the mice maintain normal vital signs during the experiment, providing a comfortable imaging environment for the mice and reducing imaging interference caused by temperature discomfort. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the structure of a fixation device for mouse brain imaging proposed in this invention;

[0030] Figure 2 This is a schematic diagram showing the connection between the first fixation component and the moving platform in a fixation device for mouse brain imaging proposed in this invention.

[0031] Figure 3 This is a schematic diagram of the brain fixation plate in a fixation device for mouse brain imaging proposed in this invention;

[0032] Figure 4 This is a schematic diagram of the structure of the L-shaped plate in a fixation device for mouse brain imaging proposed in this invention;

[0033] Figure 5 This is a schematic diagram of the moving platform in a fixation device for mouse brain imaging proposed in this invention;

[0034] Figure 6 This is a schematic diagram of the heating pad structure in a fixation device for mouse brain imaging proposed in this invention;

[0035] Figure 7 This is a schematic diagram of the structure of the second fixation component in a fixation device for mouse brain imaging proposed in this invention;

[0036] Figure 8 This is a schematic diagram showing the connection between the fixing stage and the sliding pressure plate in a fixing device for mouse brain imaging proposed in this invention.

[0037] Figure 9 This is a schematic diagram of the structure of the first adjustment component in a fixation device for mouse brain imaging proposed in this invention;

[0038] Figure 10 This is a schematic diagram showing the connection between the height adjustment component, the second adjustment platform, and the third adjustment platform in a fixation device for mouse brain imaging proposed in this invention.

[0039] Figure 11 This is a schematic diagram showing the connection between the height adjustment component, the second adjustment platform, and the third adjustment platform in a fixation device for mouse brain imaging proposed in this invention.

[0040] Figure 12 This is a schematic diagram of the internal structure of the moving platform in a fixation device for mouse brain imaging proposed in this invention;

[0041] In the attached figures: 1. First fixing component; 10. L-shaped plate; 101. First connecting groove; 102. First connecting hole; 11. Supporting cylinder; 12. First connecting plate; 13. Brain fixation piece; 130. Positioning hole; 131. Second connecting groove; 2. Adjustment platform; 20. Installation platform; 21. First adjustment platform; 22. Second adjustment platform; 23. First adjustment knob; 24. Second adjustment knob; 25. Third adjustment knob; 26. Third adjustment platform; 27. Height adjustment component; 271. Guide groove; 272. Rotating plate; 273. Fixing frame; 274. Connecting rod; 28. Lead screw; 29. ​​Gear; 3. Mounting base plate; 4. First adjustment assembly; 40. L-shaped bracket; 41. Connector; 42. Second connecting plate; 5. Heating pad; 6. Second fixing assembly; 61. Third connecting plate; 62. Support plate; 63. Sliding groove; 64. Connecting platform; 641. Fifth fixing hole; 642. Sliding groove; 65. Sliding pressure plate; 66. Fixing rod; 7. Third fixing assembly; 70. First fixing groove; 71. Second fixing groove; 8. Adjustment hole. Detailed Implementation

[0042] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0043] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0044] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0045] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0046] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0047] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0048] See Figures 1-10 This invention proposes a fixation device for mouse brain imaging. The fixation device includes a first fixation component 1 for fixing the mouse head. The bottom of the first fixation component 1 is connected to an adjustment platform 2 for adjusting the tilt angle and position. The bottom of the adjustment platform 2 is connected to a mounting base plate 3. A second fixation component 6 for fixing the mouse body is disposed on one side of the adjustment platform 2 on the mounting base plate 3, and one end of the second fixation component 6 is close to the first fixation component 1. In this device, the first fixation component 1 achieves efficient and stable fixation of the mouse head, preventing movement during mouse brain imaging and improving the accuracy and reliability of imaging.

[0049] See Figures 1-4The first fixing component 1 includes a U-shaped plate 10, which is mounted on the top of the adjustment platform 2. The U-shaped plate 10 has four first connecting holes 102, each for mounting screws. The screws securely fix the U-shaped plate 10 to the top of the adjustment platform 2. Two first connecting grooves 101 are symmetrically arranged on the upper surface of the U-shaped plate 10 near the second fixing component 6. Each first connecting groove 101 penetrates the bottom end face of the U-shaped plate 10. A second fixing screw is installed inside, and a support cylinder 11 is fixedly connected to the top end of the second fixing screw. The bottom end face of the support cylinder 11 is in contact with the upper end face of the T-shaped plate 10. The second fixing screw and the support cylinder 11 are connected by threads. Thus, by adjusting the second fixing screw, the support cylinder 11 can be moved down the T-shaped plate 10 along the direction of the third adjusting groove. And when the support cylinder 11 is damaged, it can be replaced by disassembling the second fixing screw, which increases the practicality of this device.

[0050] See Figures 1-4 Each supporting cylinder 11 has a first connecting plate 12 connected to its top end. A second adjusting groove is provided on the first connecting plate 12 at the position corresponding to the end of the supporting cylinder 11. A first fixing screw is installed in the second adjusting groove, and the bottom end of the first fixing screw is fixed to the top end face of the supporting cylinder 11. The first fixing screw and the supporting cylinder 11 are connected by threads, facilitating the disassembly of the first connecting plate 12 and the supporting cylinder 11, thus facilitating the assembly of the device. A first adjusting groove is provided on the end of the first connecting plate 12 away from the second adjusting groove, and the first adjusting groove and the second connecting groove 131 are connected by a threaded rod. A head fixing plate 13 is provided near its end... A second connecting groove 131 is provided, and a fixed threaded rod is installed between the second connecting groove 131 and its corresponding first adjusting groove, so that the brain fixation plate 13 is connected between the two first connecting plates 12. The brain fixation plate 13 is fixed to the mouse head with a sufficient amount of dental cement and glue mixture. The brain fixation plate 13 is provided with a positioning hole 130, which is used to locate the viewing window area of ​​the mouse brain imaging. The positioning hole 130 is set in an inverted cone shape. The positioning hole 130 can avoid shadows in the imaging area due to the thickness of the fixation plate, which would affect the experimental observation. During the process of fixing the mouse head, the positioning hole 130 on the brain fixation plate 13 is aligned with the mouse brain area to be observed.

[0051] See Figure 1 , Figure 2 , Figure 5 , Figure 10 , Figure 11 and Figure 12The adjustment platform 2 includes an installation platform 20, which is fixedly installed on the installation base plate 3. The top end of the installation platform 20 is connected to a first adjustment table 21. A first angle adjustment component is provided between the installation platform 20 and the first adjustment table 21. A first adjustment knob 23 is connected to the angle adjustment component and is rotatably installed on the installation platform 20.

[0052] A third adjustment platform 26 is installed on the top of the first adjustment platform 21. A second angle adjustment component is installed between the first adjustment platform 21 and the third adjustment platform 26. A second adjustment knob 24 is connected to the second angle adjustment component. The second adjustment knob 24 is rotatably mounted on the first adjustment platform 21.

[0053] Both the second and first angle adjusting components include a lead screw 28. A lead screw 28 is installed in both the mounting platform 20 and the first adjusting table 21. One end of the lead screw 28 in the mounting platform 20 is connected to the first adjusting knob 23, and one end of the lead screw 28 in the first adjusting table 21 is connected to the second adjusting knob 24. Teeth 29 are provided on the bottom surface of the first adjusting table 21 corresponding to the position of the lead screw 28. Teeth 29 are also provided on the bottom of the third adjusting table 26 corresponding to the position of the lead screw 28. The lead screw 28 meshes with the corresponding teeth 29. First arc-shaped grooves are provided on both sides of the lead screw 28 in the mounting platform 20. A first arc-shaped platform is provided on the bottom end face of the adjusting platform 21 at the position corresponding to the first arc-shaped groove. The first arc-shaped platform is movably installed in the first arc-shaped groove. Rotating the lead screw 28, through the teeth 29 and the engagement of the first arc-shaped platform with the first arc-shaped groove, causes the first adjusting platform 21 to rotate on the mounting platform 20. Second arc-shaped grooves are provided on both sides of the first adjusting platform 21. A second arc-shaped platform is provided on the bottom end face of the third adjusting platform 26 at the position corresponding to the second arc-shaped groove. The second arc-shaped platform is movably installed in the second arc-shaped groove. Rotating the lead screw 28, through the engagement of the teeth 29 and the engagement of the second arc-shaped platform with the second arc-shaped groove, causes the first adjusting platform 21 to rotate on the third adjusting platform 26.

[0054] A second adjusting platform 22 is mounted on the top of the third adjusting platform 26. A height adjusting component 27 is installed between the second adjusting platform 22 and the third adjusting platform 26. A third adjusting knob 25 is connected to the height adjusting component 27. The third adjusting knob 25 is rotatably mounted on the first adjusting platform 21. The top of the second adjusting platform 22 is connected to the L-shaped plate 10. By rotating the third adjusting knob 25, the height adjusting component 27 is activated, adjusting the distance between the second adjusting platform 22 and the third adjusting platform 26, thereby raising the second adjusting platform 22 to a certain height. The height adjusting component 27 includes a fixing frame 273. A mounting cavity is provided on the upper end face of the third adjusting platform 26. The fixing frame 273 is installed on the inner wall of the mounting cavity. One end of a connecting rod 274 is vertically movably inserted into the fixing frame 273. The other end of 4 is fixed to the bottom end face of the second adjustment platform 22. The connecting rod 274 is L-shaped. A connecting post is provided on the end of the connecting rod 274 near the fixed frame 273. The connecting post is movably installed in the guide groove 271. The guide groove 271 is set on the rotating plate 272. The rotating plate 272 is fixedly installed on the inner end of the third adjustment knob 25. By rotating the third adjustment knob 25, the rotating plate 272 is rotated, causing the connecting post to move in the guide groove 271, thereby causing the connecting rod 274 to move vertically in the mounting cavity and lift the second adjustment platform 22. A telescopic rod is provided on the top end face of the third adjustment platform 26 on the side of the mounting cavity. One end of the telescopic rod is fixed to the top end face of the third adjustment platform 26, and the other end of the telescopic rod is fixed to the bottom end of the second adjustment platform 22.

[0055] See Figure 1 , Figure 6 , Figure 7 , Figure 8 and Figure 9The second fixing component 6 includes a support plate 62 for placing the mouse body. Multiple sets of fixing components are mounted on the upper end face of the support plate 62, arranged side-by-side. Each set of fixing components includes two connecting platforms 64. Sliding grooves 63 are symmetrically arranged on the upper end face of the support plate 62. The connecting platforms 64 are installed within the sliding grooves 63, and each connecting platform 64 has a fifth fixing hole 641. A fixing rod 66 is installed within the fifth fixing hole 641, with one end of the fixing rod 66 tightly attached to the mounting base plate 3. Furthermore, the mouse body is reinforced by wrapping it with restraint straps through the sliding grooves 63. After the connecting platforms 64 are adjusted according to the mouse's body position within the sliding grooves 63, they are fixed by the fixing rod 66. When fixing the mouse body, the mouse body is positioned between two connecting platforms 64 in each group. One connecting platform 64 in each group of fixing components is provided with a groove 642, and one end of a sliding pressure plate 65 is connected to the other connecting platform 64. The other end of the sliding pressure plate 65 passes through the groove 642 and is fixed by a fixing rod 66. Multiple threaded holes are evenly spaced on the sliding pressure plate 65 near the groove 642. One of the threaded holes is connected to one end of the fixing rod 66, so that the sliding pressure plate 65 fixes the mouse body. By adjusting the sliding pressure plate 65, this device can be adapted to fix mice of different sizes, achieving comfortable and secure fixation of the mouse body, while ensuring the comfort and safety of the mouse during the imaging process.

[0056] See Figure 1 and Figure 7 A third fixing component 7 is provided on the support plate 62 near the end of the brain fixation plate 13. The third fixing component 7 includes a first fixing groove 70 and a second fixing groove 71. The first fixing groove 70 is used to insert the lower jaw of the mouse after the mouse's mouth is open, and the second fixing groove 71 is used to hold the mouse's incisors. Together with the first fixing component 1, the mouse's head can be fixed more stably, providing accuracy and reliability for the mouse's brain imaging and preventing the mouse from struggling to get rid of the brain fixation plate 13.

[0057] See Figure 1 , Figure 7 , Figure 8 and Figure 9A first adjustment component 4 is connected to the side end face of the support plate 62 opposite to the connecting platform 64. The first adjustment component 4 is mounted on the mounting base plate 3. The first adjustment component 4 includes an L-shaped bracket 40. The mounting base plate 3 is provided with multiple adjustment holes 8, which are symmetrically arranged about the central axis of the mounting base plate 3. The L-shaped bracket 40 is provided with a first fixing hole. A threaded rod is installed in the first fixing hole of the L-shaped bracket 40 and connected to its corresponding adjustment hole 8 through the threaded rod to fix the L-shaped bracket 40 to the mounting base plate 3. The position of the support plate 62 can be adjusted by connecting the first fixing hole of the L-shaped bracket 40 to different adjustment holes 8 through threaded rods. After the mouse head is fixed by the first adjustment component 4, the position of the support plate 62 is adjusted through the adjustment holes 8, thereby stably fixing the mouse body on the support plate 62. A second adjustment component 4 is provided on the side wall of the L-shaped bracket 40. A second fixing hole is provided, in which a connector 41 is installed. A second connecting plate 42 is connected to the connector 41. A third fixing hole is provided on the second connecting plate 42. The connector 41 connects the second connecting plate 42 to the side end face of the L-shaped bracket 40 through the third fixing hole and the second fixing hole. Another connector 41 is also installed on the third fixing hole. A third connecting plate 61 is connected to the third connecting plate 61. A fourth fixing hole is provided on the third connecting plate 61. The connector 41 connects the third connecting plate 61 to the side end face of the second connecting plate 42 through the third fixing hole and the fourth fixing hole. The upper end of the third connecting plate 61 is fixed to the side end face of the support plate 62. The first adjustment component 4 allows the support plate 62 to be angled on the mounting base plate 3, so that the mouse can be comfortably fixed on the support plate 62, reducing the resistance of the mouse and improving the stability of molding.

[0058] See Figure 1 , Figure 6 and Figure 7 A heating pad 5 is provided on the support plate 62. The heating pad 5 ensures that the mouse maintains normal vital signs during the experiment, provides a comfortable imaging environment for the mouse, and reduces imaging interference caused by temperature discomfort.

[0059] The operating method of this device is as follows: Anesthetize mice to different degrees or in different ways according to experimental requirements. Place the anesthetized mouse through the connecting platform 64 onto the heated pad 5 with the power on. After opening the mouse's mouth, the first fixing slot 70 is used to insert the mouse's lower jaw, and the second fixing slot 71 is used to hold the mouse's incisors, achieving initial fixation of the mouse's head. Adjust the sliding pressure plate 65 on the connecting platform 64 to a suitable position. Use the fixing rod 66 to fix the sliding pressure plate 65 to fix the mouse's body. Fix the positioning hole 130 of the brain fixation plate 13 to the imaging area of ​​the mouse's brain using dental cement. According to experimental needs, adjust the X, Y, and Z directions of the adjustment platform 2 by turning the first adjustment knob 23, the second adjustment knob 24, and the third adjustment knob 25, and adjust the second fixing component 6 to adjust the relative position of the L-shaped plate 10 and the supporting cylinder 11, so that the brain fixation plate 13 can be reasonably fixed on the first connecting plate 12 through the positioning hole 130, thereby completing the fixation of the mouse.

[0060] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the scope of the invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0061] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can be appropriately combined to form other embodiments that can be understood by those skilled in the art. The above content is only for illustrating the technical concept of the present invention and should not be construed as limiting the scope of protection of the present invention. Any modifications made based on the technical concept proposed in this invention shall fall within the scope of protection of the claims of this invention.

Claims

1. A fixation device for imaging of a mouse brain, characterized in that The utility model provides a mouse brain imaging device, including the first fixed component (1) for fixing mouse head, the bottom of first fixed component (1) is connected with the adjusting platform (2) for adjusting the angle of inclination and position, the bottom of adjusting platform (2) is connected with the installation bottom plate (3), the one side of installation bottom plate (3) is provided with the second fixed component (6) for fixing mouse body at the position of adjusting platform (2), and one end of second fixed component (6) is close to first fixed component (1); The first fixed component (1) includes the type board (10), the type board (10) is installed on the top of adjusting platform (2), two support cylinders (11) are symmetrically arranged on the type board (10) close to the position of second fixed component (6), the top of each support cylinder (11) is movably connected with first connecting plate (12), brain fixing piece (13) is connected between two first connecting plates (12), brain fixing piece (13) is fixed on the head of mouse, and positioning hole (130) is arranged on brain fixing piece (13), and the positioning hole (130) is used for positioning the window area of mouse brain imaging; The second fixed component (6) includes the support plate (62) for placing mouse body, a plurality of sets of fixed components are installed on the upper end of support plate (62), each set of fixed components includes two connecting tables (64), and mouse body is located between two connecting tables (64) in each set, wherein, one connecting table (64) in each set of fixed components is provided with sliding groove (642), one end of sliding pressing piece (65) is connected to the other connecting table (64), and the other end of sliding pressing piece (65) is fixed through fixing rod (66) by penetrating sliding groove (642), so that sliding pressing piece (65) fixes mouse body; The first adjusting assembly (4) is connected to the side end face of connecting table (64) on support plate (62), and the adjusting assembly (4) is installed on installation bottom plate (3); The support plate (62) is symmetrically provided with sliding groove (63), and connecting table (64) is installed in sliding groove (63), fifth fixing hole (641) is arranged on connecting table (64), and fixing rod is installed in fifth fixing hole (641), and one end of fixing rod is close to installation bottom plate (3); The third fixing piece (7) is arranged on the support plate (62) close to the end of brain fixing piece (13), and the third fixing piece (7) includes first fixing groove (70) and second fixing groove (71), the first fixing groove (70) is used for wearing and placing the lower jaw of mouse after the mouth of mouse is opened, and the second fixing groove (71) is used for clamping and placing the incisor of mouse.

2. The fixation device for imaging mouse brain of claim 1, wherein, The second connecting groove (131) is arranged on the brain fixing piece (13) close to the end thereof, the first adjusting groove is arranged on the first connecting plate (12) corresponding to the end of brain fixing piece (13), and the first adjusting groove and the second connecting groove (131) are connected through connecting lead screw.

3. The fixation device for imaging mouse brain of claim 2, wherein, The first connecting plate (12) is provided with a second adjusting groove at the end away from the first adjusting groove, and a first fixing screw is installed in the second adjusting groove, and the bottom end of the first fixing screw is fixed to the top of the supporting cylinder (11).

4. The fixation device for imaging mouse brain of claim 1, wherein, The fourth plate (10) is provided with a third adjusting groove at the end close to the second fixing assembly (6), and a second fixing screw is connected in the third adjusting groove, and the top end of the second fixing screw is fixed to the top of the supporting cylinder (11).

5. The fixation device for imaging mouse brain of claim 1, wherein, The adjusting platform (2) comprises a mounting platform (20) fixedly installed on the mounting bottom plate (3), a first adjusting table (21) installed on the top of the mounting platform (20), a first angle adjusting piece arranged between the mounting platform (20) and the first adjusting table (21), and a first adjusting knob (23) connected to the first angle adjusting piece and rotatably installed on the mounting platform (20). The top of the first adjusting table (21) is provided with a third adjusting table (26), a second angle adjusting piece is arranged between the third adjusting table (26) and the first adjusting table (21), a second adjusting knob (24) is connected to the second angle adjusting piece and rotatably installed on the first adjusting table (21). The top of the third adjusting table (26) is provided with a second adjusting table (22), a height adjusting piece (27) is arranged between the second adjusting table (22) and the third adjusting table (26), a third adjusting knob (25) is connected to the height adjusting piece (27) and installed on the third adjusting table (26).

6. The fixation device for imaging mouse brain of claim 1, wherein, The first adjusting assembly (4) comprises an L-shaped support (40) installed on the mounting bottom plate (3), a connecting piece (41) connected to one side of the L-shaped support (40), a second connecting plate (42) connected to the connecting piece (41), a third connecting plate (61) connected to the second connecting plate (42), and the third connecting plate (61) is fixed to the side end face of the supporting plate (62).

7. The fixation device for imaging mouse brain of claim 6, wherein, The mounting bottom plate (3) is provided with a plurality of adjusting holes (8), and the L-shaped support (40) and the corresponding adjusting hole (8) are connected by a threaded rod.

8. The fixation device for imaging mouse brain of claim 1, wherein, The supporting plate (62) is provided with a heating pad (5).

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