Oil-control anti-inflammatory soothing plant composition

Abstract

The invention provides a plant composition capable of controlling oil, resisting inflammation and relieving. The composition comprises the following raw materials in parts by mass: 3-5 parts of a centella asiatica extract, 1-2 parts of a liquorice extract, 2-4 parts of a clerodendranthus spicatus extract, 1-4 parts of a willow herb extract, 2-5 parts of a trifolium pretense flower extract, 4-6 parts of a cymbopogon citratus leaf extract, 7-9 parts of a multiflora rose root extract and 0.1-0.5 part of a vitamin compound. The multiflora rose roots are extracted by adopting reflux extraction, enzymolysis and fermentation technologies, active substances in the multiflora rose roots are fully extracted, the obtained multiflora rose root extract has a synergistic effect with other raw materials, and excellent oil control, anti-inflammation and soothing effects are provided for skin.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic preparations, and particularly relates to an oil-controlling, anti-inflammatory and soothing plant composition. Background Art

[0002] With the accelerated pace of life in modern society and the intensification of environmental pollution, people are facing more and more pressure, which not only affects people's mental health, but also has a significant impact on skin health. Especially in terms of oil secretion, it has become the focus of many people. Therefore, the research and development of cosmetics with oil control, anti-inflammatory and soothing functions has become an important research direction in the current cosmetics industry. Among the many effective ingredients for acne removal and oil control, natural plant extracts have attracted the attention of researchers for their high biosafety and bioactivity.

[0003] However, in the process of extracting and applying plant extracts, how to maintain the stability and effectiveness of its active ingredients is a technical problem that needs to be solved. Especially for wild rose root extract, due to its complex composition and high difficulty in extraction, how to use scientific and effective extraction methods to improve its extraction efficiency and the stability of active ingredients has become a key issue in cosmetic research and development. Summary of the invention

[0004] The invention provides an oil-controlling, anti-inflammatory and soothing plant composition, which has the effects of oil control, anti-inflammatory and soothing through natural ingredients extracted from plants.

[0005] In order to achieve the above-mentioned purpose, the present invention provides an oil-controlling, anti-inflammatory and soothing composition, and improves the extraction efficiency and the stability of the active ingredients through a specific extraction method, especially an enzymatic fermentation extraction method for wild rose root extract. At the same time, the present invention also provides a resident cosmetic comprising the oil-controlling, anti-inflammatory and soothing composition, which has good oil-controlling, anti-inflammatory and soothing effects, is safe and mild to use, and is suitable for people of all skin types.

[0006] The present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides an oil-controlling, anti-inflammatory and soothing composition, comprising the following raw materials in parts by weight:

[0008] 3-5 parts of Centella asiatica extract;

[0009] 1-2 parts of licorice extract;

[0010] 2-4 parts of kidney tea extract;

[0011] 1-4 parts of Epilobium angustifolium extract;

[0012] 2-5 parts of red clover flower extract;

[0013] 4-6 parts of lemon bahao leaf extract;

[0014] 7-9 parts of wild rose root extract;

[0015] Vitamin complex 0.1-0.5 parts;

[0016] The vitamin complex consists of beta-carotene, lutein, vitamin C and vitamin E, and the mass ratio of beta-carotene, lutein, vitamin C and vitamin E is 1:0.5-0.7:3-6:0.1-0.2.

[0017] In a second aspect, the present invention provides a method for preparing a wild rose root extract, the specific steps of which are as follows:

[0018] T1, drying the wild rose root to constant weight, crushing the wild rose root using a micro airflow crushing device to obtain A1, and sealing and storing for later use;

[0019] T2, reflux extraction of A1 with water-saturated ethyl acetate, filtering to obtain extract A2 and residue A3, wherein the mass ratio of water-saturated ethyl acetate to A1 is 20-50:1;

[0020] T3, concentrating and freeze-drying A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0021] T4, drying A3 to constant weight at 79-85°C, crushing to obtain A5, adding deionized water twice the mass of A5, stirring evenly to obtain a mixed solution, using cellulase, ligninase, and pectinase to enzymolyze the mixed solution, and filtering after enzymolysis for 2 hours to obtain a filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1-3, and the total mass of cellulase, ligninase, and pectinase is 8%-10% of the mass of A5;

[0022] T5, fermenting A6 with red yeast, filtering, and obtaining fermentation product A7, wherein the fermentation time is 48-72 hours, the initial fermentation pH is 5.5-6.5, the fermentation temperature is 25-35° C., and the amount of red yeast is more than 10% of the mass of A6;

[0023] T6, subjecting the fermentation product A7 to ultrasonic disruption for 10 min, filtering and sterilizing to obtain A8;

[0024] T7. Mix A4 and A8 to obtain the wild rose root extract A9.

[0025] In a third aspect, the present invention further provides a leave-on cosmetic comprising the following components in percentage by weight:

[0026] 1-8wt% oil-controlling, anti-inflammatory and soothing composition, 6-10wt% honey, 0.1-0.3wt% betaine, 0.1-1wt% sodium lauryl sulfate, 1-4wt% sodium hyaluronate, and the balance is deionized water.

[0027] The preparation method of the leave-on cosmetics comprises the following specific steps:

[0028] X1: Add honey, betaine and sodium lauryl sulfate to deionized water, heat to 60-65℃, and homogenize for 0.5h;

[0029] X2: Cool down to 25°C-45°C, add the oil-controlling, anti-inflammatory and soothing composition and sodium hyaluronate, and homogenize for 2 hours to obtain the cosmetics.

[0030] The present invention innovatively compounds Centella asiatica extract, Glycyrrhiza uralensis extract, Herba Angustifolia extract, Angustifolia herb extract, Trifolium pratense flower extract, Lemon Bahao leaf extract and Rosa multiflora root extract to obtain an oil-controlling, anti-inflammatory and soothing composition, wherein for the preparation of the Rosa multiflora root extract, reflux extraction is first performed using water-saturated ethyl acetate to extract volatile components of flavonoids, phenols and esters in the Rosa multiflora root extract; then cellulase, ligninase and pectinase are used to enzymolyze the reflux extraction residue to convert cellulose into sugars and provide a carbon source for subsequent fermentation; Then, the enzymatic hydrolyzate is fermented with red yeast to obtain red yeast metabolites, and finally the reflux extract is mixed with the fermentation metabolites to obtain wild rose root extract, which retains the active substances in the wild rose root as much as possible. At the same time, red yeast metabolites are also added. Experimental verification shows that the wild rose root extract obtained can be effectively enhanced in its antibacterial, soothing, oil-control, and acne-removing effects when compounded with Centella asiatica extract, smilax glabra extract, tea of ​​kidney tea, angustifolia extract, red clover flower extract, and lemon bahao leaf extract, and has a synergistic effect.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention adopts the technology of first reflux extraction and then enzymatic fermentation to extract wild rose root, which not only retains the activity of easily oxidized and volatile substances, but also obtains polysaccharide compounds through enzymatic hydrolysis, and obtains biological fermentation products, yeast metabolites and polysaccharides through fermentation. It can be seen that by adopting the extraction process of the present invention, the original active substances of wild rose root can be effectively retained, and more other natural active substances can be introduced, thereby effectively improving the natural activity of wild rose root extract. In addition, the present invention uses the prepared wild rose root extract in combination with Centella asiatica extract, licorice extract, kidney tea extract, willow herb extract, red clover flower extract, lemon bahao leaf extract, and vitamin complex, which has a synergistic effect and provides excellent oil control, anti-inflammatory and soothing effects for the skin. DETAILED DESCRIPTION

[0032] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0033] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0034] Some raw materials and their sources are as follows:

[0035] Centella asiatica extract: purchased from Shaanxi New Horizon Biotechnology Co., Ltd.;

[0036] Licorice extract: purchased from Fufeng Snot Biotechnology Co., Ltd.;

[0037] Kidney tea extract: purchased from Fufeng Snort Biotechnology Co., Ltd.;

[0038] Epilobium angustifolium extract: purchased from Fufeng Snot Biotechnology Co., Ltd.;

[0039] Red clover flower extract: purchased from Fufeng Snot Biotechnology Co., Ltd.;

[0040] Lemon Bahao leaf extract: purchased from Kangluoxin (Guangdong) Biotechnology Co., Ltd.;

[0041] Wild rose root: Hongsheng Professional Chinese Medicinal Herbs Planting Cooperative in Qiaocheng District, Bozhou City;

[0042] Wild rose flower: Bozhou Bailintang Pharmaceutical Co., Ltd.;

[0043] Cellulase: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TSENZ1548, 500,000 U / g;

[0044] Ligninase: purchased from Hebei Hongtao Bioengineering Co., Ltd., model: 80498-15-3, 40 U / mg;

[0045] Pectinase: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TSENZ1027, 40 U / mg;

[0046] Rhodotorula glutinosae: purchased from Ningbo Testo Biotechnology Co., Ltd., model: TS357009;

[0047] Yeast was purchased from Ningbo Testo Biotechnology Co., Ltd., model: TS372626.

[0048] Preparation of Rosa multiflora root extract:

[0049] Embodiment 1:

[0050] T1, drying the wild rose root to constant weight, crushing the wild rose root using a micro airflow crushing device to obtain A1, and sealing and storing for later use;

[0051] T2, using water-saturated ethyl acetate to reflux extract A1, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0052] T3, concentrating and freeze-drying A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0053] T4, drying A3 to constant weight at 82°C, crushing to obtain A5, adding deionized water twice the mass of A5, stirring evenly to obtain a mixed solution, using cellulase, ligninase, and pectinase to enzymolyze the mixed solution, and filtering after enzymolysis for 2 hours to obtain a filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:2, and the total mass of cellulase, ligninase, and pectinase is 9% of the mass of A5;

[0054] T5, fermenting A6 with red yeast, filtering, and obtaining fermentation product A7, wherein the fermentation time is 60 hours, the initial fermentation pH is 5.8, the fermentation temperature is 30° C., and the amount of red yeast is 10% of the mass of A6;

[0055] T6, subjecting the fermentation product A7 to ultrasonic disruption for 10 min, filtering and sterilizing to obtain A8;

[0056] T7. Mix A4 and A8 to obtain the wild rose root extract A9.

[0057] Embodiment 2:

[0058] T1, drying the wild rose root to constant weight, crushing the wild rose root using a micro airflow crushing device to obtain A1, and sealing and storing for later use;

[0059] T2, using water-saturated ethyl acetate to reflux extract A1, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0060] T3, concentrating and freeze-drying A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0061] T4, drying A3 to constant weight at 85°C, crushing to obtain A5, adding deionized water twice the mass of A5, stirring evenly to obtain a mixed solution, using cellulase, ligninase, and pectinase to enzymolyze the mixed solution, and filtering after enzymolysis for 2 hours to obtain a filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1, and the total mass of cellulase, ligninase, and pectinase is 10% of the mass of A5;

[0062] T5, fermenting A6 with red yeast, filtering, and obtaining fermentation product A7, wherein the fermentation time is 48 hours, the initial fermentation pH is 6.0, the fermentation temperature is 26° C., and the amount of red yeast is 10% of the mass of A6;

[0063] T6, subjecting the fermentation product A7 to ultrasonic disruption for 10 min, filtering and sterilizing to obtain A8;

[0064] T7. Mix A4 and A8 to obtain the wild rose root extract A9.

[0065] Embodiment 3:

[0066] T1, drying the wild rose root to constant weight, crushing the wild rose root using a micro airflow crushing device to obtain A1, and sealing and storing for later use;

[0067] T2, using water-saturated ethyl acetate to reflux extract A1, filtering to obtain extract A2 and residue A3, wherein the reflux extraction temperature is 50°C, the reflux extraction time is 1.5h, and the mass ratio of water-saturated ethyl acetate to A1 is 40:1;

[0068] T3, concentrating and freeze-drying A2 to obtain A4, which is then sealed, protected from light, and frozen for later use;

[0069] T4, drying A3 to constant weight at 79°C, crushing to obtain A5, adding deionized water twice the mass of A5, stirring evenly to obtain a mixed solution, using cellulase, ligninase, and pectinase to enzymolyze the mixed solution, and filtering after enzymolysis for 2 hours to obtain a filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:3, and the total mass of cellulase, ligninase, and pectinase is 8% of the mass of A5;

[0070] T5, fermenting A6 with red yeast, filtering, and obtaining fermentation product A7, wherein the fermentation time is 72 hours, the initial fermentation pH is 6.3, the fermentation temperature is 33° C., and the amount of red yeast is 10% of the mass of A6;

[0071] T6, subjecting the fermentation product A7 to ultrasonic disruption for 10 min, filtering and sterilizing to obtain A8;

[0072] T7. Mix A4 and A8 to obtain the wild rose root extract A9.

[0073] Comparative Example 1: The wild rose roots in Example 1 were replaced with wild rose flowers, and the other steps and parameters were the same as those in Example 1.

[0074] Comparative Example 2: The mass ratio of cellulase, ligninase and pectinase in Example 1 was changed to 1:3:2, and the other steps and parameters were the same as those in Example 1.

[0075] Comparative Example 3: The red yeast in Example 1 was replaced with Saccharomyces cerevisiae, and the other steps and parameters were the same as those in Example 1.

[0076] Comparative Example 4: The ultrasonic crushing step of T6 in Example 1 was deleted, and the other steps and parameters were the same as those in Example 1.

[0077] The raw materials and their weight proportions of an oil-controlling, anti-inflammatory and soothing composition are shown in Table 1.

[0078] Table 1 Raw materials and their mass proportions of the oil-controlling, anti-inflammatory and soothing composition

[0079]

[0080]

[0081]

[0082] Note: The preparation method of the wild rose root extract (alcohol extraction) in Table 1 is as follows: the raw material is A1 in Example 1, and A1 is refluxed and extracted with 65wt% ethanol at 50°C for 1.5h. After the reflux extraction, the mixture is filtered, and the filtrate is freeze-dried to obtain the wild rose root extract (alcohol extraction), wherein the mass ratio of A1 to 65wt% ethanol is 1:40.

[0083] The vitamin complex in Table 1 consists of β-carotene, lutein, vitamin C and vitamin E, wherein the mass ratio of β-carotene, lutein, vitamin C and vitamin E is 1:0.6:4:0.2.

[0084] The component composition of a leave-on cosmetic is shown in Table 2, and the specific preparation steps are as follows:

[0085] X1: Add honey, betaine and sodium lauryl sulfate to deionized water, heat to 63°C, and homogenize for 0.5h;

[0086] X2: Cool down to 30° C., add the oil-controlling, anti-inflammatory and soothing composition and sodium hyaluronate, and homogenize for 2 hours to obtain the cosmetics.

[0087] Table 2 Components and mass percentages of leave-on cosmetics

[0088]

[0089] Safety Test

[0090] With reference to the 2015 Technical Specifications for Safety of Cosmetics, the cosmetics in Examples 1-7 were evaluated for irritation using a skin patch test on 70 randomly distributed people aged 16-65 years.

[0091] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0092] Evaluation criteria:

[0093] Grade 0: negative reaction;

[0094] Grade 1: Suspicious reaction, only slight erythema;

[0095] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0096] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0097] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0098] Test results: Skin reactions were negative in all subjects.

[0099] The above test results show that the cosmetics containing the oil-controlling, anti-inflammatory and soothing composition provided by the present invention are mild and non-irritating to the skin and safe to use.

[0100] Propionibacterium acnes inhibition test

[0101] Referring to WS / T650-2019, the inhibition ring test was used to wash the 24h fresh slant culture of P. acnes with PBS and dilute it to 5.0×10 5 CFU / mL-5.0×10 6 CFU / mL is reserved.

[0102] Preparation of antibacterial sheets: Cut filter paper sheets with a diameter of 5 mm and a thickness not exceeding 4 mm, and immerse each group of 4 sheets in 10 mL of a sterile aqueous solution of compositions 1-5 and compositions ①-⑨ with a concentration of 1 mg / L.

[0103] Preparation of negative control samples: Take samples of the same material, make discs (blocks) of the same size as the test group, and soak them in sterile water.

[0104] Use a sterile cotton swab to apply 5.0×10 5 CFU / mL-5.0×10 6 CFU / mL acne bacteria suspension is evenly applied on the surface of a suitable culture medium plate for 3 times. Each time it is applied, the plate should be rotated 60°, and finally the cotton swab should be applied around the edge of the plate. Cover the plate and dry it at room temperature for 5 minutes. For each test, place 1 infected plate, 4 test samples and 1 negative control sample on each plate, for a total of 5 samples. Use sterile tweezers to take the sample and paste it on the surface of the plate. The negative control sample is pasted in the center of the plate, and the test sample is pasted around. After pasting, use sterile tweezers to gently press the sample to make it close to the surface of the plate. The distance between the centers of each sample is more than 25mm, and the distance from the periphery of the plate is more than 15mm. Cover the plate, incubate at 36℃±1℃ for 16h~18h to observe the results, and repeat the test 3 times. Use a vernier caliper to measure the diameter of the antibacterial ring (including the patch) and record it. When measuring the antibacterial ring, a uniform and completely sterile antibacterial ring should be selected, and the diameter should be measured based on the outer edge of the antibacterial ring.

[0105] The negative control sample should not produce an antibacterial ring. If the diameter of the antibacterial ring of the test sample is greater than 7mm, it is judged to have an antibacterial effect; if the diameter of the antibacterial ring is ≤7mm, it is judged to have no antibacterial effect. If the antibacterial effect is found in 3 repeated tests, it is judged to be qualified. See Table 3 for specific results.

[0106] Table 3 Propionibacterium acnes inhibition test

[0107] Experimental Group Inhibition zone size / mm Composition 1 19.82 Composition 2 21.22 Composition 3 17.84 Composition 4 15.73 Composition 5 15.43 Composition ① 15.52 Composition ② 14.34 Composition ③ 15.17 Composition ④ 13.75 Composition ⑤ 11.18 Composition ⑥ 7.83 Composition ⑦ 3.04 Composition⑧ 12.22 Composition ⑨ 14.33

[0108] The results show that in terms of antibacterial performance, compositions 1-5 are superior to compositions ①-⑨, and there is a certain synergistic effect between the components in the specified proportions of the present invention.

[0109] Detection of lipid accumulation in SZ95 cells by Nile red staining

[0110] (1) Cell inoculation: SZ95 cells with good growth status were collected, digested with trypsin and centrifuged, and then resuspended in DMEM complete medium and the cell concentration was adjusted to 3×10 5 12-well plate, add sterile cell slides to each well, evenly seed 2 mL of cell suspension in each well, and then place it in a 37°C, 5% CO2 incubator for incubation;

[0111] (2) After the cells adhered to the wall, discard the old culture medium and wash twice with PBS. Testosterone (6ug / ml) and linoleic acid (100μM) were used to induce lipid synthesis. A model group (no sample added) and a sample group (2μL, 1mg / L of composition 1-5 and composition ①-⑨) were set up respectively, and then placed in a 37°C, 5% CO2 incubator for further incubation for 24h;

[0112] (3) After incubation for 24 hours, discard the culture medium, wash twice with cold PBS, and fix with 4% paraformaldehyde at room temperature for 10 minutes. After fixation, stain with Nile red dye (100 μg / mL) and incubate in a 37°C incubator for 10 minutes, remove the dye, then wash with PBS 3-5 times, take out the cell slide and place it on a slide, and observe the staining under a fluorescence microscope. Cells with strong orange-red fluorescence are lipid-rich positive cells;

[0113] (4) ImageJ software was used to analyze the fluorescence intensity of the collected photos.

[0114] SPSS 19.0 statistical software package was used for analysis. The results are shown in Table 4.

[0115] Table 4 Lipid accumulation test results

[0116] Experimental Group Cumulative degree / % Composition 1 27.35 Composition 2 27.32 Composition 3 29.17 Composition 4 31.27 Composition 5 32.34 Composition ① 39.11 Composition ② 34.25 Composition ③ 49.38 Composition ④ 83.17 Composition ⑤ 72.26 Composition ⑥ 88.81 Composition ⑦ 84.27 Composition⑧ 73.25 Composition ⑨ 35.66 Model Group 100

[0117] Result analysis: From the results of compositions 1-5, it can be seen that the oil-controlling, anti-inflammatory and soothing composition provided by the present invention has a good oil-control effect, especially compositions 1-2 have a better effect. Therefore, for cost-effectiveness considerations, composition 2 is selected as the optimal embodiment.

[0118] From the results of composition 2 and compositions ①-④, it can be seen that the composition containing the wild rose root extract extracted by the preparation method of the present invention has a good oil control effect, and any change in the process steps specified in the present invention cannot achieve the effect achieved by the present invention.

[0119] From the results of composition 2 and compositions ⑤-⑧, it can be seen that the various raw material components in the oil-controlling, anti-inflammatory and soothing composition provided by the present invention have a certain synergistic effect, and the effect achieved by the present invention cannot be achieved by omitting or replacing any one of them.

[0120] From the results of composition 2 and composition ⑨, it can be seen that the wild rose root extract prepared by the present invention has a better oil control effect than the traditional alcohol-extracted wild rose root extract.

[0121] Human efficacy trials

[0122] Oil control efficacy test

[0123] The oil control efficacy of leave-on cosmetics corresponding to Examples 1-7 and blank application cases according to T / ZHCA002-2018 test method was used to evaluate the oil control efficacy of the leave-on cosmetics, and 80 randomly distributed people aged 16-45 years were tested.

[0124] Test method:

[0125] Preliminary preparation: The test site is the forehead. The sample application area and the control area are randomly distributed on the left and right sides of the forehead. The sample application area and the control area are consistent in area, each area is at least 3cmx3cm, and the area interval is at least 1cm to ensure that the sample application area and the control area are statistically balanced.

[0126] Before the test, the subjects sat quietly in a standard test environment for at least 20 minutes, were not allowed to drink water or beverages, had their foreheads exposed, remained relaxed, and avoided touching the test area.

[0127] Test: Follow the instructions for use of the skin surface sebum tester. Under the supervision of the tester, the subject cleans the test area with an alkaline soap-based cleaning product, rinses it with clean water, and then dries it with a non-chip absorbent dry paper towel. Within 3 minutes, measure the amount of sebum on the skin surface of the sample application area and the control area respectively, and measure 3 times at different locations in each area. The measurement result is expressed as the average of 3 measurements as the initial value.

[0128] Sample at (2.0 ± 0.1) mg / cm 2 Apply a single application of the required amount, take quantitative samples using a syringe or equivalent, use a latex finger cot to evenly apply the sample to the specified area, and record the actual amount of sample applied.

[0129] At the set measurement time point, measurements were taken three times at different locations in the sample application area and the control area to obtain the amount of sebum on the skin surface of each area. The measurement results were tabulated as the average value of the three measurements.

[0130] The interval between set test time points should be no less than 1 hour. Multiple test time points can be set according to evaluation needs. The entire test cycle usually does not exceed 24 hours.

[0131] When measuring at different measurement time points, avoid locations that have already been measured.

[0132] During the test, the subject's forehead was exposed and touching the tested area was avoided.

[0133] The same tester must use the same instrument to complete the cosmetic test for the same subject. If the subject has an adverse reaction to the skin during the use of the sample, the test will be terminated and the subject will be given appropriate medical treatment. Adverse reactions should be recorded.

[0134] Result statistics: Comparison and statistics of the measured values ​​of the sample smear area and the control area. The specific data are shown in Table 5.

[0135] formula: Where A0 is the mean value of sebum volume test in the control area, and A is the mean value of sebum volume test in the test area.

[0136] Table 5 Oil control efficacy test results

[0137] Experimental Group Oil output inhibition rate Application Example 1 65.24％ Application Example 2 52.27％ Application Example 3 31.56％ Application Example 4 63.72％ Application Example 5 62.26％ Application Example 6 61.22％ Application Example 7 62.75％ Blank Application Example 9.33％

[0138] Result analysis: From the results of Application Examples 1-7, it can be seen that the oil-controlling, anti-inflammatory and soothing composition provided in the embodiments of the present invention has a good oil-control effect, indicating that the use of the addition amount specified in the present invention can better exert its oil-control effect.

[0139] Soothing effect test

[0140] This test verifies the soothing effect of leave-on cosmetics of Application Examples 1-7 and Blank Application Example on damaged skin.

[0141] Specifically, volunteers were used for testing: 80 testers were recruited and divided into 8 groups of 10 people each, with half male and half female in each group, aged 20-35 years old. The test method was as follows:

[0142] (1) Tape irritation test: The tape was repeatedly applied to the inner skin of the forearm 15 times to damage the skin;

[0143] (2) Each tester applied 0.25 g of application examples 1-7 and blank application example on their left hand;

[0144] (3) Observe and evaluate the results after 15 minutes.

[0145] Scoring: A 5-point system was used, with 1 point - more severe redness; 2 points - no improvement; 3 points - slight improvement, but obvious redness is still visible; 4 points - redness has basically disappeared; 5 points - redness has completely disappeared. The results are shown in Table 6.

[0146] Table 6 Soothing effect test results

[0147] Experimental Group Average rating for soothing Application Example 1 4.9 Application Example 2 4.9 Application Example 3 4.8 Application Example 4 4.6 Application Example 5 4.7 Application Example 6 4.6 Application Example 7 4.5 Blank Application Example 2.4

[0148] Human patch test

[0149] Histamine was used as a stimulus to evaluate the improvement effect of the samples on symptoms such as erythema and itching of histamine-sensitized skin.

[0150] Test samples: Application Examples 1-7 and blank application examples.

[0151] Volunteers and test environment: 8 volunteers with normal skin, aged 20-30 years old, with random genders were selected; the test environment was 25±2℃ and humidity was 60%RH.

[0152] Test method: Wash the inner side of the subject's arm with clean water and blow dry with cold air for 15-20 minutes; apply 20μL of 1% histamine solution on the patch sticker and stick it to the inner side of the volunteer's arm. Remove the sticker after 30 minutes, mark the patch position with a marker, and apply different samples to be tested, and record the time it takes for the erythema and itching to subside at the patch position.

[0153] The test results are shown in Table 7.

[0154]

[0155]

[0156] As shown in Tables 6-7, Application Examples 1-7 have significant anti-inflammatory and soothing effects compared to the blank application examples.

[0157] The "compositions", "application examples" and "embodiments" described above are part of the embodiments of the present application, but not all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

Claims

1. An oil-controlling, anti-inflammatory and soothing composition, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: 3-5 parts of Centella asiatica extract; 1-2 parts of licorice extract; 2-4 parts of kidney tea extract; 1-4 parts of Epilobium angustifolium extract; 2-5 parts of red clover flower extract; 4-6 parts of lemon bahao leaf extract; 7-9 parts of wild rose root extract; Vitamin complex 0.1-0.5 parts; The vitamin complex consists of β-carotene, lutein, vitamin C and vitamin E, and the mass ratio of β-carotene, lutein, vitamin C and vitamin E is 1:0.5-0.7:3-6:0.1-0.2; The method for extracting the wild rose root extract is specifically enzymatic fermentation extraction, and the steps are as follows: T1, drying and crushing the root of wild rose to obtain A1; T2, using water-saturated ethyl acetate to reflux extract A1, filtering to obtain extract A2 and residue A3, wherein the mass ratio of water-saturated ethyl acetate to A1 is 20-50:1; T3, concentrating and freeze-drying A2 to obtain A4, which is then sealed, protected from light, and frozen for later use; T4, drying A3 to constant weight at 79-85°C, crushing to obtain A5, adding water twice the mass of A5, stirring evenly to obtain a mixed solution, using cellulase, ligninase, and pectinase to enzymolyze the mixed solution, and filtering after enzymolysis for 2 hours to obtain a filtrate A6, wherein the mass ratio of cellulase, ligninase, and pectinase is 1:2:1-3, and the total mass of cellulase, ligninase, and pectinase is 8%-10% of the mass of A5; T5, fermenting A6 with red yeast, filtering, and obtaining fermentation product A7, wherein the fermentation time is 48-72 hours, the initial fermentation pH is 5.5-6.5, the fermentation temperature is 25-35° C., and the amount of red yeast is more than 10% of the mass of A6; T6, subjecting the fermentation product A7 to ultrasonic disruption for 10 min, filtering and sterilizing to obtain A8; T7. Mix A4 and A8 to obtain the wild rose root extract A9.

2. The oil-controlling, anti-inflammatory and soothing composition according to claim 1, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: 3-4 parts of Centella asiatica extract; 1-2 parts of licorice extract; 2-3 parts of kidney tea extract; 1-3 parts of Epilobium angustifolium extract; 2-4 parts of red clover flower extract; 4-5 parts of lemon bahao leaf extract; 7-8 parts of wild rose root extract; Vitamin complex 0.3-0.5 parts.

3. The oil-controlling, anti-inflammatory and soothing composition according to claim 1, characterized in that: The oil-controlling, anti-inflammatory and soothing composition comprises the following raw materials in parts by weight: Centella asiatica extract 4 parts; 1 part of licorice extract; 3 parts of kidney tea extract; 3 parts of Epilobium angustifolium extract; 4 parts of red clover flower extract; 5 parts of lemon bahao leaf extract; 8 parts of wild rose root extract; Vitamin complex 0.3 parts.

4. A leave-on cosmetic, characterized in that: The invention comprises the oil-controlling, anti-inflammatory and soothing composition according to any one of claims 1 to 3.

5. The cosmetic according to claim 6, characterized in that: The cosmetic comprises the following components in percentage by weight: 1-8% of an oil-controlling, anti-inflammatory and soothing composition, 6-10% of honey, 0.1-0.3% of betaine, 0.1-1% of sodium lauryl sulfate, 1-4% of sodium hyaluronate, and the remainder is deionized water.

6. The cosmetic according to claim 7, characterized in that: The cosmetic preparation steps are as follows: X1: Add honey, betaine and sodium lauryl sulfate to deionized water, heat to 60-65℃, and homogenize for 0.5h; X2: Cool down to 25°C-45°C, add the oil-controlling, anti-inflammatory and soothing composition and sodium hyaluronate, and homogenize to obtain the cosmetics.

Images