A moisturizing, oil-controlling and soothing plant composite skin care composition, and a preparation method and application thereof

Abstract

The present application relates to a kind of moisturizing, oil control and soothing plant composite skin care composition and its preparation method, application, belong to cosmetic raw material technical field.The moisturizing, oil control and soothing plant composite skin care composition is composed of white white lily flower extract, honeysuckle flower extract and hamamelis virginiana extract with weight ratio (4-6) : (2-3) : (1-3) ;The extraction process and matching ratio of raw materials in the composition of the present application are optimized, and the composition is endowed with excellent skin moisturizing, oil control and skin soothing effect, and has good application effect in cosmetics.

Description

Technical Field

[0001] This invention relates to a moisturizing, oil-controlling and soothing plant-based composite skincare composition, its preparation method, and its application, belonging to the field of cosmetic raw material technology. Background Technology

[0002] Plant extracts refer to one or more active ingredients in plants that are obtained and concentrated through physical or chemical methods (such as solvent extraction, distillation, etc.) using the roots, stems, leaves, flowers, fruits, etc. They have a wide range of applications in cosmetics.

[0003] White water lilies are aquatic plants belonging to the Nymphaeaceae family, renowned for their elegant white flowers. Water lilies are often used to treat minor skin inflammation, redness, and burns due to their excellent soothing properties. Furthermore, they help the skin establish a hydrating mechanism, increasing the moisture content of the epidermis, giving the skin elasticity, and leaving it with a dewy, clear appearance. They provide a gentle calming effect, reducing redness and discomfort, and also have a certain astringent effect, making them suitable for sensitive or irritated skin. They can also help reduce pigmentation caused by inflammation (such as UV rays or acne). Honeysuckle, also known as Lonicera japonica, is traditionally considered a good medicine for clearing heat and detoxifying. Its extract is rich in caffeic acid and luteolin, which have excellent antibacterial and anti-inflammatory effects, helping to inhibit bacterial growth on the skin surface and reduce acne and inflammation. In addition, it contains components that eliminate free radicals, preventing skin aging and protecting skin cells from oxidative damage. Furthermore, substances such as luteolin in its extract have strong skin penetration and the ability to absorb ultraviolet rays, helping to inhibit and eliminate skin pigmentation, especially age spots.

[0004] Witch hazel, a shrub native to North America, is widely used in traditional medicine for its bark and leaves, often referred to as "witch hazel." As a gentle natural astringent, it helps control sebum production by shrinking pores, reducing facial oiliness and thus conditioning the skin. It causes a slight coagulation of proteins on the skin's surface, creating an astringent effect and temporarily narrowing the sebum secretion channels at the pore openings, reducing shine. Furthermore, it helps balance sebum production, reducing oil production at its source. It also protects the skin from UV damage and pollutants, and promotes skin regeneration and wound healing, making it suitable for acne-prone or damaged skin. While plant extracts are highly favored in cosmetics due to their natural origin and diverse functions (such as moisturizing, anti-inflammatory, antioxidant, and whitening), their complex active ingredients, the uncertainty in adjusting extraction processes and the resulting efficacy, and the uncertainty in optimizing the combination of plant extracts all limit the application of plant extract compositions in cosmetics. Therefore, improving extraction processes and the types of raw materials used to optimize the efficacy of active ingredients in plant extract compositions remain technical challenges that need to be addressed. Summary of the Invention

[0005] To address the technical problems mentioned in the background section, this invention provides a moisturizing, oil-controlling, and soothing plant-based composite skincare composition, its preparation method, and its applications. The composition of this invention is obtained by compounding three plant extracts, specifically white water lily extract, honeysuckle flower extract, and witch hazel extract. Furthermore, this invention optimizes the combination and extraction process of the plant extracts, significantly enhancing the efficacy of the composition.

[0006] Specifically, the present invention relates to a moisturizing, oil-controlling and soothing plant-based complex skin care composition, which is composed of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of (4-6): (2-3): (1-3).

[0007] Preferably, the extracts of white water lily and honeysuckle are obtained by adding 60-80% ethanol to the flowers to break the cell walls, then heating and extracting, centrifuging, filtering, concentrating, adding ethyl acetate for extraction, standing, concentrating and drying.

[0008] Preferably, the North American witch hazel extract is obtained by fermentation of Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus branii CICC 6131, followed by ethanol extraction, heating, centrifugation, filtration, concentration, and drying.

[0009] Preferably, the composition comprises white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 6:3:1.

[0010] Preferably, the composition consists of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 4:3:3.

[0011] Preferably, the composition comprises white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 5:2.5:2.5.

[0012] As a preferred embodiment, the preparation method of white water lily extract includes: taking white water lily, adding 4-30 times the weight of 60-80% ethanol to break the cell wall, heating and extracting at 30-50℃ for 0.5-5 hours, centrifuging, filtering, concentrating to obtain a concentrate, adding 3-10 times the weight of ethyl acetate to the concentrate, stirring and extracting at 20-35℃ for 0.5-3 hours, allowing to stand, and taking the ethyl acetate extract phase for concentration and drying to obtain the extract.

[0013] As a preferred embodiment, the preparation method of honeysuckle flower extract includes: taking honeysuckle flowers, adding 4-30 times the weight of 60-80% ethanol to break the cell wall, heating and extracting at 30-50℃ for 0.5-5 hours, centrifuging, filtering, concentrating to obtain a concentrate, adding 3-10 times the weight of ethyl acetate to the concentrate, stirring and extracting at 20-35℃ for 0.5-3 hours, allowing to stand, and taking the ethyl acetate extract phase for concentration and drying to obtain the final product.

[0014] As a preferred embodiment, the preparation method of North American witch hazel extract includes: taking leaves of North American witch hazel, adding water to break the cell wall to obtain broken material, adding carbon source and nitrogen source, sterilizing to obtain a mixture, inoculating with Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC 6131, sealing and fermenting, sterilizing, adding ethanol, heating and extracting, centrifuging, filtering, concentrating and drying to obtain the extract.

[0015] Preferably, in the preparation method of North American witch hazel extract, the ratio of *Lactobacillus rhamnosus* CGMCC 1.2467 to *Lactobacillus fragrans* CICC 6131 is 0.5-1.5:1, the fermentation temperature and time are 20-40℃ for 2-5 days, and the amount of ethanol added is 0.8-1.2 times the weight of water added. Based on the weight of the mixture, the total viable bacteria count of *Lactobacillus rhamnosus* CGMCC 1.2467 and *Lactobacillus fragrans* CICC 6131 used for inoculation is 1×10⁻⁶. 10 -9×10 10 The ratio of bacteria to extracts is 0.5-1.5:1, which can enhance the richness of active ingredients and the oil-controlling ability of the extract. The extraction temperature and time are 30-40℃ for 2-6 hours.

[0016] Preferably, in the preparation method of North American witch hazel extract, the water added for cell wall disruption is 3-30 times the weight of the leaves. The carbon source includes sugars, including but not limited to at least one of glucose, sucrose, fructose, and maltose. The nitrogen source includes organic nitrogen and / or inorganic nitrogen, including but not limited to at least one of peptone, yeast extract, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, and disodium hydrogen phosphate. The amounts of carbon source and nitrogen source used are 0.1-3% and 0.001-0.3% of the weight of the crushed material, respectively.

[0017] The present invention also provides a method for preparing a moisturizing, oil-controlling and soothing plant-based skincare composition, comprising mixing white water lily extract, honeysuckle flower extract and witch hazel extract, and then packaging.

[0018] The present invention also provides the application of white water lily extract and honeysuckle flower extract in a weight ratio of (4-6):(2-3) or between 4:3 and 6:2 in synergistic enhancement and soothing of the skin.

[0019] This invention also provides the application of the aforementioned moisturizing, oil-controlling, and soothing plant-based complex skincare composition in the preparation of cosmetics, wherein the composition is used in cosmetics at an amount of 0.1-20 wt%. Cosmetics include at least one of lotion, serum, foundation, cream, ointment, and mask.

[0020] The advantages of this invention compared to existing technologies are: This invention uses white water lily extract, honeysuckle flower extract and North American witch hazel extract in a weight ratio of (4-6):(2-3):(1-3) to prepare a composition. This invention optimizes the extraction process and matching ratio of the raw materials in the composition, giving the composition excellent skin moisturizing, oil control and skin soothing effects, and has good application effect in cosmetics.

[0021] The extract obtained by using 60-80% ethanol for cell wall disruption and heating extraction of white water lily and honeysuckle flower, followed by ethyl acetate extraction, exhibits excellent soothing effects. Furthermore, the combination of the two extracts obtained under the above extraction method shows a specific effect. Research in this invention shows that tests reveal an antagonistic effect between the white water lily extract and the honeysuckle flower extract at a ratio of 0.1:1, a simple additive effect at ratios of 0.3:1, 0.8:1, 5:1, and 10:1, and a synergistic effect between ratios of 4:3 to 6:2. The combination of the two extracts synergistically enhances the skin-soothing effect.

[0022] This invention utilizes witch hazel leaves, which undergoes cell wall disruption with water, followed by co-fermentation with *Lactobacillus rhamnosus* CGMCC 1.2467 and *Lactobacillus glutenans* CICC 6131, and then extraction with ethanol solution. The resulting extract exhibits excellent oil-controlling properties. The extraction process of this invention has significant advantages: for extraction from witch hazel leaves, the combined process of "microbial fermentation + ethanol extraction" is crucial for enhancing the oil-controlling activity of the extract; furthermore, not all fermentation strains can achieve the same effect as the specific fermentation strains used in this invention, and the combination of *Lactobacillus rhamnosus* CGMCC 1.2467 and *Lactobacillus glutenans* CICC 6131 in this invention has specific advantages. Furthermore, the co-fermentation of Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC 6131 in this invention also exhibits a synergistic effect, which can more effectively enhance the oil control ability of the extract when they work together. Moreover, when using Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC 6131 for co-fermentation, it is necessary to select a ratio of strain quantity (0.5-1.5:1) to obtain the best oil control effect. Detailed Implementation

[0023] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0024] The preparation method of white water lily (Nymphaea alba L.) flower extract is as follows: Take 300g of white water lily flower and add 20 times its weight of 72.5wt% ethanol for cell wall disruption. The cell wall disruption conditions are: 20000rpm, run for 30 seconds and stop for 10 seconds, with a total running time of 4 minutes. Then, heat and extract in a stirred reactor at 45℃ and 100rpm for 3 hours. Centrifuge at 3000rpm for 25 minutes. Filter the supernatant through a 0.22-micron filter membrane. Concentrate the filtrate under reduced pressure at 50℃ until the condensate has no alcohol odor to obtain the concentrate. Add 4.5 times the weight of ethyl acetate to the concentrate and stir at 30℃ and 120rpm for 1.5 hours. Let stand at room temperature for 0.5 hours. Concentrate the ethyl acetate extract under reduced pressure at 35℃ and dry under vacuum at 40℃ to constant weight to obtain the white water lily extract.

[0025] The preparation method of honeysuckle (LONICERA JAPONICA) flower extract is as follows: Take 320g of honeysuckle flowers and add 25 times the weight of 65wt% ethanol for cell wall disruption. The cell wall disruption conditions are: 20000rpm, run for 30 seconds and stop for 10 seconds, with a total running time of 4 minutes. Then, heat and extract in a stirred reactor at 45℃ for 2.5 hours, centrifuge at 3000rpm for 25 minutes, and filter the supernatant through a 0.22-micron filter membrane. Concentrate the filtrate under reduced pressure at 50℃ until the condensate has no alcohol odor to obtain the concentrate. Add 5.5 times the weight of ethyl acetate to the concentrate and stir at 28℃ and 150rpm for 2.5 hours. Let it stand at room temperature for 0.5 hours, and concentrate the ethyl acetate extract under reduced pressure at 35℃ and dry it under vacuum at 40℃ to constant weight to obtain the honeysuckle flower extract.

[0026] Example 1 The test solution was prepared according to Table 1, measured by weight. The weight ratio in Table 1 represents the weight ratio of white water lily extract to honeysuckle flower extract. The diluent was a solution composed of distilled water, glycerin, and Tween-80 in a weight ratio of 90:9:1.

[0027] Table 1: Test solutions The skin-soothing properties of the above-mentioned solution were tested, and the testing process is as follows: Test basis: "T / SHRH034-2021 Test of soothing efficacy of cosmetics - In vitro TNF-α inflammatory factor content determination method for lipopolysaccharide-induced macrophage cell line inflammatory cell model".

[0028] Consumables and reagents: mouse cells RAW264.7 (logarithmic growth phase), DMEM medium, TNF-α assay kit, microplate reader, lipopolysaccharide LSP, dexamethasone sodium phosphate.

[0029] The corresponding reagents for each group were as follows: Blank group: culture medium; Negative group: culture medium supplemented with 0.5 μg / mL LPS; Test group: culture medium supplemented with 0.5 μg / mL LPS and 5 wt% test solution; Positive control group: culture medium supplemented with 0.5 μg / mL LPS and 100 μg / mL dexamethasone sodium phosphate.

[0030] Experimental procedure: RAW264.7 cells were seeded into 96-well plates using culture medium at a density of 100 μL per well and a cell count of 1 × 10⁶ cells / well. 5Each well was incubated at 37℃ and 5% CO2 for 24 hours. The culture medium was then discarded, and 100 μL of the corresponding reagent was added according to the grouping. Each group had 5 replicates. The wells were then incubated at 37℃ and 5% CO2 for 24 hours. After incubation, the absorbance of each well was measured at 450 nm according to the instructions of the TNF-α test kit to obtain the TNF-α content and TNF-α inhibition rate. The reduction in TNF-α content was calculated as: TNF-α content in the negative group - TNF-α content in the test group or positive control group. The results are shown in Table 2.

[0031] Table 2: TNF-α levels and decrease values

[0032] According to the test results in Table 2, the extract obtained by using 60-80% ethanol for cell wall disruption and heating extraction of white water lily and honeysuckle flower, followed by ethyl acetate extraction, has excellent soothing effects. Furthermore, the combination of the two obtained under the above extraction method exhibits a specific effect, as follows: Test solutions 2-10 are combinations of the two in ratios of 0.1:1, 0.3:1, 0.8:1, 4:3, 1.8:1, 2.2:1, 6:2, 5:1, and 10:1. The tests show an antagonistic effect at a ratio of 0.1:1, a simple additive effect at ratios of 0.3:1, 0.8:1, 5:1, and 10:1, and a synergistic effect between ratios of 4:3 and 6:2. The combination of the two can synergistically enhance the skin-soothing effect.

[0033] Example 2 Extracts of North American witch hazel (HAMAMELIS VIRGINIANA) were prepared using different processes, and the preparation methods involved are as follows: North American witch hazel extract 1: Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus branii CICC 6131 were inoculated at a live bacteria count ratio of 0.5:1. The mixture was stirred at 60 rpm and fermented in a sealed container at 30°C for 3.5 days. After sterilization, anhydrous ethanol (0.9 times the weight of water) was added, and the mixture was heated at 35°C and 100 rpm for 2.5 hours for extraction. The mixture was then centrifuged at 3000 rpm for 20 minutes. The supernatant was filtered through a 0.22-micron filter membrane. The filtrate was concentrated under reduced pressure at 50°C until the condensate had no alcohol odor, and then freeze-dried at -45°C to constant weight.

[0034] North American witch hazel extract 2: Leaves of North American witch hazel were taken and mixed with water at 18 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4.5 minutes. The resulting material was then mixed with 1.2% sucrose source and 0.15% peptone by weight, sterilized, and a mixture was prepared. The mixture was then formulated with a total viable count of 5.7 × 10⁻⁶. 10 Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus branii CICC 6131 were inoculated at a live bacteria count ratio of 1.5:1. The mixture was stirred at 60 rpm and fermented at 35°C for 3 days. After sterilization, anhydrous ethanol (1 times the weight of water) was added and extracted by heating at 35°C and 100 rpm for 2 hours. The mixture was then centrifuged at 3000 rpm for 25 minutes. The supernatant was filtered through a 0.22-micron filter membrane. The filtrate was concentrated under reduced pressure at 50°C until the condensate had no alcohol odor, and then freeze-dried at -50°C to constant weight.

[0035] North American witch hazel extract 3: No fermentation process; Leaves of North American witch hazel were taken and water with 15 times the weight of the leaves was added to break the cell walls. The cell wall breaking conditions were: 20,000 rpm, running for 30 seconds and stopping for 10 seconds, with a total running time of 4 minutes to obtain the broken material. Anhydrous ethanol with 0.9 times the weight of the water was added and the mixture was heated and extracted at 35°C and 100 rpm for 2.5 hours. The mixture was then centrifuged at 3000 rpm for 20 minutes. The supernatant was filtered through a 0.22-micron filter membrane. The filtrate was concentrated under reduced pressure at 50°C until the condensate had no alcohol odor, and then freeze-dried at -45°C to constant weight.

[0036] North American witch hazel extract 4: Ethanol-free extraction process; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus branii CICC 6131 were inoculated at a live bacteria count ratio of 0.5:1. The mixture was stirred at 60 rpm and fermented in a sealed container at 30°C for 3.5 days. After sterilization, the mixture was centrifuged at 3000 rpm for 20 minutes. The supernatant was collected and filtered through a 0.22-micron filter membrane. The filtrate was then freeze-dried at -45°C to constant weight.

[0037] North American witch hazel extract 5: fermented with other bacteria; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Inoculate *Saccharomyces cerevisiae* CICC 31361 at a ratio of 1 / g, mix thoroughly at 60 rpm, and ferment at 30°C for 3.5 days. After sterilization, add anhydrous ethanol at 0.9 times the weight of the water mentioned above, heat and extract at 35°C and 100 rpm for 2.5 hours, centrifuge at 3000 rpm for 20 minutes, collect the supernatant and filter through a 0.22-micron filter membrane. Concentrate the filtrate under reduced pressure at 50°C until the condensate has no alcohol odor, and then freeze-dry at -45°C to constant weight.

[0038] North American witch hazel extract 6: fermented by inoculation with a fermentation bacteria; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Inoculate *Lactobacillus rhamnosus* CGMCC 1.2467 at a ratio of 1 / g, mix at 60 rpm, and ferment at 30°C for 3.5 days. After sterilization, add anhydrous ethanol at 0.9 times the weight of water, heat and extract at 35°C and 100 rpm for 2.5 hours, centrifuge at 3000 rpm for 20 minutes, collect the supernatant and filter through a 0.22-micron filter membrane. Concentrate the filtrate under reduced pressure at 50°C until the condensate has no alcohol odor, and then freeze-dry at -45°C to constant weight.

[0039] North American witch hazel extract 7: fermented by inoculation with a fermentation bacteria; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Inoculate *Lactobacillus fragrans* CICC 6131 at a ratio of 1 / g, mix thoroughly at 60 rpm, and ferment at 30°C for 3.5 days. After sterilization, add anhydrous ethanol at 0.9 times the weight of the aforementioned water, heat and extract at 35°C and 100 rpm for 2.5 hours, centrifuge at 3000 rpm for 20 minutes, collect the supernatant, filter through a 0.22-micron filter membrane, concentrate the filtrate under reduced pressure at 50°C until the condensate has no alcohol odor, and then freeze-dry at -45°C to constant weight.

[0040] North American witch hazel extract 8: Changes in bacterial ratio; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC 6131 were inoculated at a live bacteria count ratio of 0.1:1. The mixture was stirred at 60 rpm and fermented in a sealed container at 30°C for 3.5 days. After sterilization, anhydrous ethanol (0.9 times the weight of water) was added, and the mixture was heated at 35°C and 100 rpm for 2.5 hours for extraction. The mixture was then centrifuged at 3000 rpm for 20 minutes. The supernatant was filtered through a 0.22-micron filter membrane. The filtrate was concentrated under reduced pressure at 50°C until the condensate had no alcohol odor, and then freeze-dried at -45°C to constant weight.

[0041] North American witch hazel extract 9: Changes in bacterial ratio; Leaves of North American witch hazel were taken and mixed with water at 15 times their weight for cell wall disruption. The disruption conditions were: 20,000 rpm, running for 30 seconds followed by a 10-second pause, for a total running time of 4 minutes. The resulting material was then mixed with 1.5% sucrose source and 0.1% peptone by weight, sterilized, and a mixture was prepared. The total viable bacteria count for the mixture was 4.5 × 10⁻⁶. 10 Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus branii CICC 6131 were inoculated at a live bacteria count ratio of 3:1. The mixture was stirred at 60 rpm and fermented at 30°C for 3.5 days. After sterilization, anhydrous ethanol (0.9 times the weight of water) was added, and the mixture was heated at 35°C and 100 rpm for 2.5 hours. After centrifugation at 3000 rpm for 20 minutes, the supernatant was filtered through a 0.22-micron filter membrane. The filtrate was concentrated under reduced pressure at 50°C until the condensate had no alcohol odor, and then freeze-dried at -45°C to constant weight.

[0042] Example 3 The oil-controlling properties of North American witch hazel extracts obtained from different processes in Example 2 were tested. The test methods are as follows: Basis: The effect of drugs on the inhibition of lipid synthesis in SZ95 cells was used to test the oil-controlling properties of the extract.

[0043] Consumables and reagents: SZ95 cells (logarithmic growth phase), culture medium (DMEM / Ham's F12 mixed in a 3:1 ratio), PBS, test sample (witch hazel extract diluted to 2 wt% with diluent consisting of distilled water, glycerol, and Tween-80 in a weight ratio of 90:9:1), flow cytometer.

[0044] Experimental procedure: Take SZ95 cells and add 2 mL of culture medium containing 10% FBS and 1% PS to each well of a plate, along with 4 × 10⁻⁶ cells. 5 Cells were cultured at 37℃ and 5% CO2 for 24 hours. Then, 2 mL of culture medium containing 1 wt‰ of the test sample was added to each well (blank medium was used for the control group). The cells were cultured at 37℃ and 5% CO2 for another 24 hours. The culture medium was discarded, and the cells were washed twice with PBS. Digestion was stopped with 0.25 wt% trypsin. The cells were then transferred to EP tubes, centrifuged, and the supernatant was discarded. 1 mL of PBS was added, and the cells were resuspended. The cell suspension was prepared by centrifugation again, discarding the supernatant, and adding another 1 mL of PBS. 10 μL of Nile Red fluorescent dye was added to each tube to a final concentration of 100 ng / mL. The cells were incubated at room temperature for half an hour, vortexed, and filtered. The average fluorescence intensity of the cells was measured using flow cytometry, and the reduction rate of fluorescence intensity compared to the control group (i.e., the inhibition rate of cell lipid synthesis) was calculated. The results are shown in Table 3. Lower fluorescence intensity indicates a stronger inhibition of lipid synthesis and a stronger oil-controlling ability.

[0045] Table 3: Fluorescence Intensity

[0046] As shown in Table 3, the extract obtained by the present invention, which involves water-induced cell wall disruption of North American witch hazel, co-fermentation with *Lactobacillus rhamnosus* CGMCC1.2467 and *Lactobacillus fragrans* CICC 6131, followed by ethanol extraction, exhibits excellent oil-controlling properties. The extraction process of the present invention has significant advantages, as detailed below: The inhibition rate of extract 3 obtained without the fermentation method of this invention was only 32.37%, and the inhibition rate of extract 4 obtained without ethanol extraction was 40.01%, both significantly lower than the effect of this invention. This indicates that for extracting witch hazel leaves, the combined process of "microbial fermentation + ethanol extraction" is crucial for improving the oil-controlling activity of the extract. The inhibition rate of extract 5 obtained by fermentation with other bacteria was 47.15%, significantly lower than the extract of this invention, indicating that not all bacterial fermentation can achieve the same effect. The combination of *Lactobacillus rhamnosus* CGMCC 1.2467 and *Lactobacillus fragrans* CICC 6131 has a specific advantage. In addition, the inhibition rates of extracts 6 and 7, both obtained by fermentation with a single bacterial species, were 53.23% and 54.14%, respectively, both lower than extract 1, and the total amount of bacteria used for fermentation was the same. This indicates that *Lactobacillus rhamnosus* CGMCC 1.2467 and *Lactobacillus fragrans* CICC 6131 have a synergistic effect, and their combined action can more effectively improve the oil-controlling ability of the extract. The bacterial ratio of extracts 8 and 9 was not within the range of 0.5-1.5:1, and the inhibition rates of the resulting extracts were 60.36% and 62.65%, respectively. Although these were higher than those of single bacterial strains, they were still lower than those of extract 1. This indicates that when using Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus fragrans CICC 6131 for co-fermentation, it is necessary to select a bacterial ratio (0.5-1.5:1) to obtain the best oil control effect.

[0047] Example 4 This embodiment relates to the preparation and application of a plant-based complex skincare composition.

[0048] Plant-based complex skincare composition 1, with raw material composition of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 6:3:1.

[0049] Plant-based complex skincare composition 2, with raw material composition of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 4:3:3 1.

[0050] Plant-based complex skincare composition 3, with raw material composition of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 5:2.5:2.5 2.

[0051] The preparation method of the above-mentioned plant-based complex skin care composition includes mixing white water lily extract, honeysuckle flower extract and witch hazel extract in a mixing container, and then packaging them.

[0052] The above composition can be used in cosmetics: Application 1: 4 parts by weight of plant-based complex skincare composition 1 and 96 parts by weight of cosmetic matrix.

[0053] Application 2: 3 parts by weight of plant-based complex skincare composition 2 and 97 parts by weight of cosmetic matrix.

[0054] Application 3: 5.5 parts by weight of plant-based complex skincare composition 3 and 94.5 parts by weight of cosmetic matrix.

[0055] Cosmetic base: Ingredients, content (parts by weight, total 100 parts): Glycerin: 5 parts; 1,3-Butanediol: 10 parts; Triethanolamine: 0.3 parts; Phenoxyethanol: 0.3 parts; Liquid paraffin: 4 parts; Vaseline: 2.5 parts; Lanolin: 2.5 parts; Stearic acid: 5 parts; Glyceryl monostearate: 3 parts; Tween-80: 2 parts; Salicylic acid: 0.08 parts; Water: balance.

[0056] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A moisturizing, oil-controlling, and soothing plant-based complex skincare composition, characterized in that, The composition consists of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of (4-6):(2-3):(1-3); Among them, the white water lily extract and the honeysuckle flower extract are obtained by adding 60-80% ethanol to the flower to break the cell wall, then heating and extracting, centrifuging, filtering, concentrating, then adding ethyl acetate for extraction, standing, concentrating and drying. North American witch hazel extract is obtained by fermentation of Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC 6131, followed by ethanol extraction, heating, centrifugation, filtration, concentration and drying.

2. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 1, characterized in that, The composition consists of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 6:3:

1.

3. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 1, characterized in that, The composition consists of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 4:3:

3.

4. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 1, characterized in that, The composition consists of white water lily extract, honeysuckle flower extract and witch hazel extract in a weight ratio of 5:2.5:2.

5.

5. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 1, characterized in that, The preparation method of white water lily extract includes: taking white water lily, adding 4-30 times the weight of 60-80% ethanol to break the cell wall, heating and extracting at 30-50℃ for 0.5-5 hours, centrifuging, filtering, concentrating to obtain concentrate, adding 3-10 times the weight of ethyl acetate to the concentrate, stirring and extracting at 20-35℃ for 0.5-3 hours, letting stand, taking the ethyl acetate extract phase for concentration and drying to obtain the extract.

6. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 1, characterized in that, The preparation method of honeysuckle flower extract includes: taking honeysuckle flowers, adding 4-30 times the weight of 60-80% ethanol to break the cell wall, heating and extracting at 30-50℃ for 0.5-5 hours, centrifuging, filtering, concentrating to obtain concentrate, adding 3-10 times the weight of ethyl acetate to the concentrate, stirring and extracting at 20-35℃ for 0.5-3 hours, letting stand, taking the ethyl acetate extract phase for concentration and drying to obtain the final product.

7. The moisturizing, oil-controlling, and soothing plant-based composite skincare composition according to claim 1, characterized in that, The preparation method of North American witch hazel extract includes: taking leaves of North American witch hazel, adding water to break the cell wall, obtaining broken cell wall material, adding carbon source and nitrogen source, sterilizing to obtain a mixture, inoculating with Lactobacillus rhamnosus CGMCC 1.2467 and Lactobacillus glutenosa CICC6131, sealing and fermenting, sterilizing, adding ethanol, heating and extracting, centrifuging, filtering, concentrating and drying to obtain the extract.

8. The plant-based composite skincare composition for moisturizing, oil control, and soothing according to claim 7, characterized in that, In the preparation method of North American witch hazel extract, the ratio of Lactobacillus rhamnosus CGMCC 1.2467 to Lactobacillus branii CICC6131 is 0.5-1.5:1, the fermentation temperature and time are 20-40℃ and 2-5 days; the amount of ethanol added is 0.8-1.2 times the weight of water added.

9. A method for preparing a plant-based composite skincare composition for moisturizing, oil control, and soothing according to any one of claims 1-8, characterized in that, This includes mixing and packaging white water lily extract, honeysuckle flower extract, and witch hazel extract.

10. The application of a moisturizing, oil-controlling, and soothing plant-based complex skincare composition according to any one of claims 1-8 in the preparation of cosmetics, characterized in that, The composition is used in cosmetics at a rate of 0.1-20 wt%.

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