A moss biochar-microorganism complex for improving saline-alkali land and improving plant salt-alkali tolerance, as well as its preparation method and application

Abstract

The present invention belongs to the technical field of soil improvement, and specifically relates to a moss biochar-microorganism consortium for improving saline-alkali soil and enhancing plant salt-alkali tolerance, as well as its preparation method and application. Azotobacteria have a nitrogen-fixing effect, and Priesteria gigantea has a potassium-dissolving effect. The present invention combines the nitrogen-fixing bacteria and Priesteria gigantea with biochar derived from moss. The moss biochar has a small pore size and can effectively adsorb metal cations in saline-alkali soils. It has a strong affinity for nitrogen-fixing bacteria and Priesteria gigantea, effectively adsorbing them and thereby enhancing their colonization on plant root surfaces and in the rhizosphere. Furthermore, the nitrogen-fixing bacteria and Priesteria gigantea attached to the biochar can alleviate saline-alkali stress in the rhizosphere microenvironment, improve crop resistance to saline-alkali environments, and promote crop growth, providing a new approach for crop stress resistance and yield increase in saline-alkali environments.

Description

Technical Field

[0001] The present invention belongs to the technical field of soil improvement, and in particular relates to a moss biochar-microorganism complex for improving saline-alkali land and enhancing the salt-alkali tolerance of plants, as well as a preparation method and application thereof. Background Art

[0002] Soil salinization is a major global challenge, causing a significant decline in available arable land and posing a significant threat to agricultural production. Improving saline-alkali land, enhancing plant resistance to salt and alkali, and achieving efficient agricultural production on saline-alkali land are major global challenges.

[0003] The classification criteria for saline-alkali land are as follows: slightly saline soil, with a salinity of 0.1-0.5%; moderately saline soil, with a salinity of 0.2-0.4%; and severely saline soil, with a salinity of 0.4-0.6%. Another indicator of saline-alkali land is soil acidity, or pH. Generally, a pH around 7.5 is neutral, below 7.0 is acidic, and above 7.5 is slightly alkaline. Soil improvement in saline-alkali land is complex due to the coexistence of salt and alkalinity. Although fertilizers for saline-alkali land improvement have been introduced nationwide, their effectiveness has been limited. Summary of the Invention

[0004] The purpose of the present invention is to provide a moss biochar-microorganism complex for improving saline-alkali land and improving the salt-alkali tolerance of plants, as well as a preparation method and application thereof, so as to effectively improve saline-alkali land and improve the salt-alkali tolerance of plants.

[0005] In order to achieve the above object, the present invention provides a moss biochar-microorganism consortium, which comprises moss biochar, nitrogen-fixing bacteria (Azotobacter sp.) and Priestia megaterium.

[0006] Preferably, the nitrogen-fixing bacteria is nitrogen-fixing bacteria BNCC335805; and the Priesteria megaterium is Priesteria megaterium NK851.

[0007] Preferably, the method for preparing the moss biochar comprises: pyrolyzing the dried moss at 500° C. for 1 to 12 hours.

[0008] The present invention also provides a method for preparing the moss biochar-microorganism complex described in the above technical solution, comprising the following steps:

[0009] The nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the moss biochar are mixed to obtain the moss biochar-microorganism complex.

[0010] Preferably, the usage ratio of the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the moss biochar is (200-300) mL: (200-300) mL: (50-150) g;

[0011] The OD of the nitrogen-fixing bacteria culture solution 600 0.5~5;

[0012] The OD of the Priesteria megaterium culture solution 600 It is 0.5 to 5.

[0013] Preferably, the method for preparing the nitrogen-fixing bacteria culture solution comprises: inoculating nitrogen-fixing bacteria into Mart culture medium, and culturing at 30° C. and 160 rpm for 24 to 48 hours to obtain the nitrogen-fixing bacteria culture solution;

[0014] The preparation method of the nitrogen-fixing bacteria culture solution comprises: inoculating Priesteria gigantea into a Mart culture medium, and culturing at 30° C. and 160 rpm for 24 to 48 hours to obtain a Priesteria gigantea culture solution;

[0015] The Mart culture medium includes 10 g / L of glucose, 5 g / L of peptone, 1 g / L of potassium dihydrogen phosphate, 0.25 g / L of magnesium sulfate, 0.01 g / L of ferrous sulfate and 5 g / L of sodium chloride.

[0016] The present invention also provides a composition comprising a substrate and a moss biochar-microorganism complex; the moss biochar-microorganism complex is the moss biochar-microorganism complex described in the above technical solution.

[0017] Preferably, the substrate comprises one or more of peat soil, bone meal, nutrient soil and bran;

[0018] Calculated based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the peat soil is 100 g:5 kg.

[0019] The present invention also provides the use of the moss biochar-microorganism consortium or composition described in the above technical solution in improving saline-alkali land and / or improving the salt-alkali tolerance of plants.

[0020] The present invention also provides a method for improving saline-alkali land and / or improving the saline-alkali tolerance of plants, comprising one or both of the following:

[0021] (1) mixing the moss biochar-microorganism complex described in the above technical solution with a matrix to obtain a moss biochar-growth-promoting microorganism complex-matrix;

[0022] applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil;

[0023] Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg;

[0024] The amount of the moss biochar-growth-promoting microorganism complex-substrate applied is 100 kg / mu;

[0025] The substrate comprises one or more of peat soil, bone meal, nutrient soil and bran;

[0026] (2) Applying the composition described in the above technical solution to saline-alkali soil; the application amount of the composition is 100 kg / mu.

[0027] Beneficial effects:

[0028] The present invention provides a moss biochar-microorganism consortium, which includes moss biochar, nitrogen-fixing bacteria and Priesteria gigantea. The nitrogen-fixing bacteria has a nitrogen-fixing effect, and Priesteria gigantea has a potassium-dissolving effect. The two strains are combined with the biochar derived from moss. The moss biochar has a small pore size and can effectively adsorb metal cations in saline-alkali soils. It has a strong affinity for nitrogen-fixing bacteria and Priesteria gigantea, and can effectively adsorb nitrogen-fixing bacteria and Priesteria gigantea, thereby enhancing the colonization of nitrogen-fixing bacteria and Priesteria gigantea on the surface of plant roots and in the rhizosphere soil; at the same time, the nitrogen-fixing bacteria and Priesteria gigantea attached to the biochar can alleviate the salt-alkali stress of the rhizosphere microenvironment, improve the resistance of crops to saline-alkali environments, and promote crop growth. The moss biochar-microorganism consortium provided by the present invention provides a new idea for crop resistance and yield increase in saline-alkali environments. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below.

[0030] Figure 1 is the adsorption rate of different biochars on different microorganisms; groups without the same letters indicate significant differences between the groups (P<0.05);

[0031] Figure 2 Soil pH values ​​after 90 days of wheat growth in different treatment groups; * indicates significant differences between the moss biochar-microbial consortium-peat soil treatment group and other groups (P<0.05);

[0032] Figure 3 The total salt content of the soil after 90 days of wheat growth in different treatment groups; * indicates that the moss biochar-microbial consortium-peat soil treatment group was significantly different from the other groups (P<0.05);

[0033] Figure 4 This is the actual picture of wheat in different treatment groups after 90 days of growth;

[0034] Figure 5 The plant height of wheat in different treatment groups after 90 days of growth; * indicates that the moss biochar-microbial consortium-peat soil treatment group was significantly different from other groups (P<0.05);

[0035] Figure 6 is the wheat yield of different treatment groups; * indicates that the moss biochar-microbial consortium-peat soil treatment group was significantly different from other groups (P<0.05);

[0036] Figure 7 The plant heights of barley in different treatment groups after 90 days of growth; * indicates significant differences between the moss biochar-microbial consortium-peat soil treatment group and other groups (P<0.05);

[0037] Figure 8 Barley yields in different treatment groups; * indicates significant differences between the moss biochar-microbial consortium-peat soil treatment group and other groups (P<0.05). DETAILED DESCRIPTION

[0038] The present invention provides a moss biochar-microorganism complex, which comprises moss biochar, nitrogen-fixing bacteria and Priesteria gigantea.

[0039] In the present invention, the preparation method of moss biochar preferably includes: pyrolyzing dried moss at 500°C for 1 to 12 hours; the pyrolysis time is preferably 2 to 10 hours, and more preferably 5 to 8 hours. The moss biochar provided by the present invention has a distinct porous structure with a small pore size, has a strong affinity for plant growth-promoting microorganisms, can effectively adsorb plant growth-promoting microorganisms, and thereby enhance the colonization of plant growth-promoting microorganisms on the surface of plant roots and in the rhizosphere soil. The results of the examples show that moss biochar has a stronger adsorption capacity for microorganisms than straw biochar.

[0040] In the present invention, the nitrogen-fixing bacteria is preferably nitrogen-fixing bacteria BNCC335805, purchased from Beina Biotechnology; the Priestia megaterium is preferably Priestia megaterium NK851, which is disclosed in the document Wu, X., Zhao, Z., Zhao, Z., Zhang, Y., Li, M., & Yu, Q. (2023). Analysis of the potassium-solubilizing Priestia megaterium strain NK851 and its potassium feldspar-binding proteins. International Journal of Molecular Sciences, 24(18), 14226. The nitrogen-fixing bacteria and Priestia megaterium of the present invention can attach to the moss biochar, alleviate the saline-alkali stress of the rhizosphere microenvironment, improve the resistance of crops to saline-alkali environments, and promote crop growth.

[0041] The present invention also provides a method for preparing the moss biochar-microorganism complex described in the above technical solution, comprising the following steps:

[0042] The nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the moss biochar are mixed to obtain the moss biochar-microorganism complex.

[0043] The present invention preferably prepares nitrogen-fixing bacteria culture solution. In the present invention, the OD of the nitrogen-fixing bacteria culture solution is 600 Preferably, it is 0.5 to 5, more preferably 1 to 4, and more preferably 2. The method for preparing the nitrogen-fixing bacteria culture solution of the present invention preferably comprises: inoculating the nitrogen-fixing bacteria into a Mart culture medium, and culturing at 30°C and 160 rpm for 24 to 48 hours to obtain a nitrogen-fixing bacteria culture solution. The Mart culture medium of the present invention preferably comprises 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate, and 5 g / L sodium chloride; the culturing time is preferably 30 to 45 hours, more preferably 36 hours.

[0044] The present invention preferably prepares a culture solution of Priesteria gigantea. In the present invention, the OD of the culture solution of Priesteria gigantea is 600Preferably, it is 0.5 to 5, more preferably 1 to 4, and more preferably 2. The method for preparing the nitrogen-fixing bacteria culture solution of the present invention preferably comprises: inoculating Priesteria gigantea into a Mart culture medium, and culturing at 30°C and 160 rpm for 24 to 48 hours to obtain a Priesteria gigantea culture solution; the Mart culture medium preferably comprises 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate, and 5 g / L sodium chloride; and the culturing time is preferably 30 to 45 hours, more preferably 36 hours.

[0045] After obtaining the nitrogen-fixing bacteria culture solution and the Priesteria gigantea culture solution, the present invention preferably mixes the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution, and the moss biochar to obtain the moss biochar-microorganism complex. In the present invention, the mixing temperature is preferably 20-30°C, more preferably 25°C; the mixing time is preferably 10-60 minutes, more preferably 20-50 minutes, and even more preferably 30 minutes.

[0046] In the present invention, the usage ratio of the nitrogen-fixing bacteria culture solution, Priesteria gigantea culture solution and moss biochar is preferably (200-300) mL: (200-300) mL: (50-150) g, and more preferably 250 mL: 250 mL: 100 g.

[0047] The present invention also provides a composition comprising a matrix and a moss biochar-microorganism complex; the moss biochar-microorganism complex is the moss biochar-microorganism complex described in the above technical solution. In the present invention, the matrix preferably comprises one or more of peat soil, bone meal, nutrient soil and bran, and is further preferably peat soil. Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar to the matrix in the moss biochar-microorganism complex of the present invention is preferably 100g:5kg. The present invention combines the matrix and the moss biochar-microorganism complex, and the matrix can adsorb and protect the moss biochar-microorganism complex, maintain the activity of the bacteria, and facilitate subsequent uniform application.

[0048] The moss biochar-microorganism consortium or composition provided by the present invention can enhance plant stress resistance in saline-alkali environments and effectively increase crop yields.

[0049] In view of the effects of the moss biochar-microorganism complex and composition provided by the present invention, the use of the moss biochar-microorganism complex or the composition of the present invention in improving saline-alkali land and / or improving the salt-alkali tolerance of plants also falls within the scope of protection of the present invention.

[0050] In the present invention, improving the salt-alkali tolerance of plants preferably includes increasing plant height and / or yield in saline-alkali soil; improving saline-alkali soil preferably includes improving the pH and / or total salt content of the saline-alkali soil; the pH value of the saline-alkali soil is preferably 7.5-9.0, more preferably 8.0-9.0; the total salinity of the saline-alkali soil is preferably 0.1%-0.3%, more preferably 0.2%-0.3%. The plants of the present invention preferably include wheat and / or barley.

[0051] The present invention also provides a method for improving saline-alkali land and / or improving the saline-alkali tolerance of plants, comprising one or both of the following:

[0052] (1) mixing the moss biochar-microorganism complex described in the above technical solution with a matrix to obtain a moss biochar-growth-promoting microorganism complex-matrix;

[0053] applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil;

[0054] Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg;

[0055] The amount of the moss biochar-growth-promoting microorganism complex-substrate applied is 100 kg / mu;

[0056] The substrate comprises one or more of peat soil, bone meal, nutrient soil and bran;

[0057] (2) Applying the composition described in the above technical solution to saline-alkali soil; the application amount of the composition is 100 kg / mu.

[0058] In the present invention, the pH value of the saline-alkali soil is preferably 7.5 to 9.0, more preferably 8.0 to 9.0; the total salinity of the saline-alkali soil is preferably 0.1% to 0.3%, more preferably 0.2% to 0.3%. In the present invention, nitrogen, phosphorus, and potassium compound fertilizer is preferably applied simultaneously with the application of the moss biochar-growth-promoting microorganism combination-matrix or composition; the total nutrient content of the nitrogen, phosphorus, and potassium compound fertilizer is preferably ≥57%, and the mass ratio of N, P2O5, and K2O is preferably 19:19:19; the application amount of the nitrogen, phosphorus, and potassium compound fertilizer is preferably 10 to 20 kg / mu, more preferably 15 kg / mu.

[0059] To further illustrate the present invention, the following detailed description of a moss biochar-microorganism complex for improving saline-alkali land and improving the salt-alkali tolerance of plants, as well as its preparation method and application is provided by the present invention in conjunction with the accompanying drawings and examples, but they should not be understood as limiting the scope of protection of the present invention.

[0060] Example 1

[0061] Preparation of moss biochar

[0062] Moss plants were collected from the wild, washed with running water to remove dust and impurities from the surface, and then oven-dried at 60°C to remove moisture. 10g of dried moss was placed in a reactor, which was then heated in a muffle furnace at 500°C for 2 hours and cooled to room temperature to produce moss biochar. Scanning electron microscopy revealed that the moss biochar had a distinct porous structure.

[0063] Comparative Example 1

[0064] Preparation of straw biochar

[0065] Straw was collected and washed with running water to remove dust and impurities from the surface. The straw was then oven-dried at 60°C to remove moisture. 10 g of dried straw was placed in a reactor, which was then placed in a muffle furnace and heated at 500°C for 2 h. The resulting straw biochar was then cooled to room temperature.

[0066] Test Example 1

[0067] Adsorption properties of biochar on microorganisms

[0068] (1) Different microorganisms were inoculated into Mart liquid medium (glucose 10 g / L, peptone 5 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate 0.25 g / L, ferrous sulfate 0.01 g / L, sodium chloride 5 g / L, pH = 8.5), cultured at 30°C, 160 rpm for 24-48 h, centrifuged at 12000 rpm for 2 min, and the precipitate was collected; the precipitate was then suspended in phosphate buffered saline (PBS) at pH = 7.2, and the OD was finally measured. 600 =0.1, and obtain bacterial suspension;

[0069] The microorganisms are Agrobacterium tumefaciens LBA4404 (denoted as LBA4404), Pseudomonas putida KT2440 (denoted as KT2440), nitrogen-fixing bacteria BNCC335805 (denoted as BNCC335805) and Priesteria gigantea NK851 (denoted as NK851);

[0070] Among them, Agrobacterium tumefaciens LBA4404 was purchased from Takara;

[0071] Pseudomonas putida KT2440 was purchased from ATCC;

[0072] Azotobacter BNCC335805 was purchased from Beina Biotechnology;

[0073] The potassium-solubilizing Priestia megaterium strain NK851 is disclosed in Wu, X., Zhao, Z., Zhao, Z., Zhang, Y., Li, M., & Yu, Q. (2023). Analysis of the potassium-solubilizing Priestia megaterium strain NK851 and its potassium feldspar-binding proteins. International Journal of Molecular Sciences, 24(18), 14226.

[0074] (2) The moss biochar of Example 1 and the straw biochar of Comparative Example 1 were placed in the bacterial suspension obtained in step (1) at a concentration of 100 mg / L. After gently shaking at 25°C for 1 hour, the biochar was separated from the mixture. The microbial cells in the remaining bacterial suspension, i.e., the microbial cells not adsorbed by the biochar, were counted on a Mart culture medium plate using the colony forming unit (CFU) counting method, and the adsorption rate was calculated. The results are shown in FIG. Figure 1 shown.

[0075] according to Figure 1 As can be seen, moss biochar has a significantly higher microbial adsorption capacity than straw biochar. Compared to straw biochar, moss biochar adsorbed over 90% of BNCC335805 and NK851. Furthermore, moss biochar adsorbed higher amounts of BNCC335805 and NK851 than did Agrobacterium tumefaciens LBA4404 and Pseudomonas putida KT2440, indicating that moss biochar has a stronger adsorption capacity for nitrogen-fixing bacteria BNCC335805 and Priesteria gigantea NK851.

[0076] Example 2

[0077] Preparation of moss biochar-symbiotic microbial consortium-peat soil matrix

[0078] (1) The nitrogen-fixing bacteria BNCC335805 was inoculated into 250 mL of Mart liquid culture medium and cultured at 30°C and 160 rpm for 24 h to obtain a nitrogen-fixing bacteria culture solution;

[0079] (2) inoculating Priesteria gigantea NK851 into 250 mL of Mart liquid culture medium, culturing at 30° C. and 160 rpm for 24 h to obtain a Priesteria gigantea culture solution;

[0080] (3) The nitrogen-fixing bacteria culture solution of step (1), the potassium-solubilizing Priesteria gigantea culture solution of step (2), and 100 g of the moss biochar obtained in Example 1 were mixed, and the mixture was gently shaken at 25° C. for 30 min to obtain a moss biochar-symbiotic microorganism complex;

[0081] (4) The moss biochar-symbiotic microorganism complex was mixed with 5 kg of peat soil matrix to obtain the moss biochar-symbiotic microorganism complex-peat soil matrix.

[0082] Comparative Example 2

[0083] Preparation of symbiotic microbial consortium-peat soil substrate

[0084] (1) The nitrogen-fixing bacteria BNCC335805 was inoculated into 250 mL of Mart liquid culture medium and cultured at 30°C and 160 rpm for 24 h to obtain a nitrogen-fixing bacteria culture solution;

[0085] (2) inoculating Priesteria gigantea NK851 into 250 mL of Mart liquid culture medium, culturing at 30° C. and 160 rpm for 24 h to obtain a Priesteria gigantea culture solution;

[0086] (3) mixing the nitrogen-fixing bacteria from step (1) and the culture solution of Priesteria gigantea from step (2) to obtain a symbiotic microbial consortium;

[0087] (4) The symbiotic microbial consortium was mixed with 5 kg of peat soil matrix to obtain a symbiotic microbial consortium-peat soil matrix.

[0088] Comparative Example 3

[0089] Preparation of moss biochar-peat soil matrix

[0090] The moss biochar obtained in Example 1 was mixed with a peat soil matrix in a mass ratio of 100 g:5 kg to obtain a moss biochar-peat soil matrix.

[0091] Test Example 2

[0092] Verification of wheat growth-promoting effect in saline-alkali soil

[0093] (1) Wheat was planted in saline-alkali land (pH 7.5-9.0, total salinity 0.2%-0.3%) in Binhai New Area, Tianjin. Specifically, the experimental site was randomly divided into four treatment areas, and the following treatments were carried out:

[0094] Treatment 1 (control group): Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied at 15 kg / mu and peat soil substrate at 100 kg / mu, and wheat was planted in rows.

[0095] In the second treatment area (symbiotic microbial consortium-peat soil matrix group), 15 kg / mu of Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the experimental site, and 100 kg / mu of the symbiotic microbial consortium-peat soil matrix (based on the weight of the peat soil matrix) of comparative example 2 was applied, and wheat was planted in rows;

[0096] Treatment 3 (moss biochar-peat soil matrix group): Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥ 57%) was applied to the soil of the experimental site at 15 kg / mu, and moss biochar-peat soil matrix (comparative example 3) was applied at 100 kg / mu (based on the weight of the peat soil matrix) and wheat was planted in rows;

[0097] The fourth treatment area (moss biochar-symbiotic microbial association-peat soil matrix group): 15 kg / mu of Stanley Sanan compound fertilizer (N-P2O5-K2O: 19-19-19, total nutrients ≥57%) and 100 kg / mu of moss biochar-symbiotic microbial association-peat soil matrix of Example 2 (based on the weight of peat soil matrix) were applied to the soil of the experimental site, and wheat was planted in rows.

[0098] (2) After 90 days of wheat planting, soil indicators, wheat growth indicators and yield of each treatment group were measured. The results are as follows: Figures 2 to 6 and as shown in Table 1.

[0099] Table 1 Wheat plant height and yield data of each treatment group

[0100]

[0101]

[0102] According to the soil test results ( Figure 2 and Figure 3 ) It can be seen that compared with the control group, the symbiotic microbial consortium-peat soil matrix treatment group and the moss biochar-peat soil matrix treatment group, the soil pH of the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group decreased significantly to only 7.85 ( Figure 2 ), the total salt content also decreased significantly, reaching 0.18% ( Figure 3 ). Therefore, the moss biochar-symbiotic microbial consortium-peat soil matrix can significantly reduce soil salinity and alkalinity, thereby improving soil quality.

[0103] According to the wheat growth index and yield test results ( Figures 4-6 and Table 1) show that the wheat growth in the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group was significantly stronger than that in the other three groups ( Figure 4), the plant height was significantly higher than the other three groups, reaching more than 51 cm ( Figure 5 and Table 1). Compared with the control group, the wheat yield of the moss biochar-symbiotic microbial consortium-peat soil substrate treatment group increased from 190 catties / mu to 483 catties / mu ( Figure 6 and Table 1). It can be seen that the moss biochar-symbiotic microorganism association can significantly promote the growth of wheat in saline-alkali soil and increase wheat yield.

[0104] Test Example 3

[0105] Verification of the growth-promoting effect on barley in saline-alkali soil

[0106] (1) The saline-alkali land (pH 8.0-9.0, total salinity 0.2%-0.3%) in Binhai New Area, Tianjin was used as the test site. Barley was planted according to the steps of Test Example 2. After 90 days of planting, the growth indexes and yield of barley were measured. The results are as follows: Figures 7-8 and as shown in Table 2.

[0107] Table 2 Barley plant height and yield data for each treatment group

[0108] Treatment group Plant height (cm) Yield (jin / mu) comparison 43±1.3 170±15 Symbiotic microbial consortium-peat soil matrix 51±2.8 286±26 Green moss biochar-peat soil matrix 50±2.0 248±15 Green moss biochar-symbiotic microbial consortium-peat soil matrix 57±3.2 382±28

[0109] according to Figure 7 、 Figure 8 As can be seen from Table 2, 90 days after barley planting, the growth of barley in the moss biochar-symbiotic microbial consortium-peat soil matrix treatment group was significantly stronger than that of the other three groups, and the plant height was significantly higher than that of the other three groups ( Figure 7 and Table 2), and barley yield was also significantly higher ( Figure 8 and Table 2). Therefore, the green moss biochar-symbiotic microorganism association can significantly promote the growth of barley in saline-alkali soil and increase its biomass.

[0110] Based on the above content, it can be seen that the moss biochar-microorganism complex provided by the present invention can improve the pH and total salt content of saline-alkali soil, improve the salt-alkali tolerance of plants, significantly promote the growth of plants in saline-alkali soil, and increase their biomass.

[0111] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A method for preparing a moss biochar-microorganism complex, characterized in that: The steps include: mixing nitrogen-fixing bacteria culture solution, Priesteria gigantea culture solution and green moss biochar to obtain the green moss biochar-microorganism complex; The preparation method of the nitrogen-fixing bacteria culture solution comprises: inoculating nitrogen-fixing bacteria (Azotobacter sp.) into a Mart culture medium, and culturing at 30° C. and 160 rpm for 24 to 48 hours to obtain the nitrogen-fixing bacteria culture solution; The preparation method of the Priestia megaterium culture solution comprises: inoculating Priestia megaterium into a Mart culture medium, and culturing at 30° C. and 160 rpm for 24 to 48 hours to obtain the Priestia megaterium culture solution; The Mart culture medium comprises 10 g / L glucose, 5 g / L peptone, 1 g / L potassium dihydrogen phosphate, 0.25 g / L magnesium sulfate, 0.01 g / L ferrous sulfate and 5 g / L sodium chloride; The usage ratio of the nitrogen-fixing bacteria culture solution, the Priesteria gigantea culture solution and the moss biochar is (200-300) mL: (200-300) mL: (50-150) g.

2. The preparation method according to claim 1, characterized in that The nitrogen-fixing bacteria is nitrogen-fixing bacteria BNCC335805; the Priesteria gigantea is Priesteria gigantea NK851.

3. The preparation method according to claim 1, characterized in that The preparation method of the moss biochar comprises: pyrolyzing dried moss at 500° C. for 1 to 12 hours.

4. The preparation method according to claim 1, characterized in that The OD of the nitrogen-fixing bacteria culture solution 600 0.5~5; The OD of the Priesteria megaterium culture solution 600 It is 0.5 to 5.

5. A composition, characterized in that The invention comprises a matrix and a moss biochar-microorganism complex; the moss biochar-microorganism complex is a moss biochar-microorganism complex obtained by the preparation method according to any one of claims 1 to 4.

6. The composition according to claim 5, characterized in that The substrate comprises one or more of peat soil, bone meal, nutrient soil and bran; Calculated based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the peat soil is 100 g:5 kg.

7. Use of the moss biochar-microorganism complex obtained by the preparation method according to any one of claims 1 to 4 or the composition according to claim 5 or 6 in improving saline-alkali land and / or improving the salt-alkali tolerance of plants.

8. The use according to claim 7, characterized in that The pH value of the saline-alkali land is 7.5-9.0, and the total salinity is 0.1%-0.3%.

9. A method for improving saline-alkali land and / or improving the salt-alkali tolerance of plants, characterized in that: Include one or both of the following: (1) mixing the moss biochar-microorganism complex obtained by the preparation method according to any one of claims 1 to 4 with a substrate to obtain a moss biochar-growth-promoting microorganism complex-substrate; applying the moss biochar-growth-promoting microorganism complex-matrix to saline-alkali soil; Based on the mass of the moss biochar in the moss biochar-microorganism complex, the mass ratio of the moss biochar-microorganism complex to the substrate is 100 g:5 kg; The amount of the moss biochar-growth-promoting microorganism complex-substrate applied is 100 kg / mu; The substrate comprises one or more of peat soil, bone meal, nutrient soil and bran; (2) Applying the composition according to claim 5 or 6 to saline-alkali soil; the application amount of the composition is 100 kg / mu.

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