Method for breaking dormancy of rice seeds

Abstract

The invention relates to the technical field of plant seed planting, in particular to a rice seed dormancy breaking method which specifically comprises the following steps: 1) seed selection: selecting rice seeds which are stored in a seed cellar at 4 DEG C and are in a dormant state; (2) soaking the rice seeds selected in the step (1) in a dormancy breaking promoting agent for 24 hours, and cleaning with clean water; and 3) carrying out a standard germination experiment at a constant temperature of 28 DEG C, and observing and recording the germination condition. The method has the advantages of being reasonable in step design, convenient to operate and remarkable in dormancy breaking effect on the rice seeds. The method can be conveniently applied to actual production, and the utilization rate and planting benefits of the seeds are improved.

Description

Technical Field

[0001] The invention relates to the technical field of plant seed planting, and in particular to a method for relieving dormancy of rice seeds. Background Art

[0002] Seed dormancy refers to the phenomenon in which viable seeds fail to germinate despite favorable germination conditions. Seed dormancy is a primitive trait accumulated through environmental adaptation. A certain degree of dormancy can effectively reduce the risk of ear sprouting during rainy weather during the rice harvest season, ensuring both rice and seed quality. In production, the dormancy of newly harvested rice varieties, coupled with varying dormancy periods, hinders off-site propagation, germination testing of seed banks, and field purity testing. Conventional germination tests often result in very low germination rates and uneven germination, leading to misjudgments and significant losses in seed production and variety sales. To objectively and effectively assess rice seed germination rates, it is necessary to explore methods for breaking dormancy in dormant rice seeds. Summary of the Invention

[0003] The object of the present invention is to provide a method for relieving dormancy of rice seeds to solve the problems raised in the above background technology.

[0004] To solve the above technical problems, the present invention provides a technical solution: a method for relieving dormancy of rice seeds, which specifically comprises the following steps:

[0005] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0006] 2) soaking the rice seeds selected in step 1) in a dormancy-breaking agent for 24 hours, and then washing with clean water; the dormancy-breaking agent is at least one of a 0.2% potassium nitrate solution, a 3% hydrogen peroxide solution, and a 0.1% gibberellin solution;

[0007] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0008] Preferably, the dormancy-release promoting agent in step 2) is a 0.2% potassium nitrate solution.

[0009] Preferably, the dormancy-release promoting agent in step 2) is a 3% hydrogen peroxide solution.

[0010] Preferably, the dormancy-release promoting agent in step 2) is a 0.1% gibberellin solution.

[0011] Preferably, the dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution and 3% hydrogen peroxide solution.

[0012] Preferably, the dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution and 0.1% gibberellin solution.

[0013] Preferably, the dormancy-release promoting agent in step 2) is a mixed solution of 3% hydrogen peroxide solution and 0.1% gibberellin solution.

[0014] Preferably, the dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution, 3% hydrogen peroxide solution, and 0.1% gibberellin solution.

[0015] The advantages of the present invention are: the steps are reasonably designed, easy to operate, and the effect of relieving dormancy of rice seeds is significant. It is highly targeted and is specially designed for rice seeds in a dormant state. Through specific processing steps, it can effectively relieve seed dormancy and improve germination rate. Seeds stored in 4°C seed cellars are selected to clarify the source and status of the seeds, making the processing method more targeted. It has good sterilization and disinfection effects. Soaking seeds with dormancy-releasing agents can effectively kill pathogens on the surface of seeds, reduce the impact of diseases on seeds during germination, and improve seed health and germination success rate. The operation is simple, and the steps of the entire processing method are simple and clear, and easy to operate. Steps such as seed selection, agent soaking, and constant temperature germination experiments are relatively easy to implement and do not require complicated equipment and technology. For rice growers, this simple and easy method can be conveniently applied to actual production to improve seed utilization and planting benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the bar graph of Table 1.

[0017] Figure 2 It is a line chart of Table 1. DETAILED DESCRIPTION

[0018] For the purpose of the following detailed description, it should be understood that the present invention may adopt various alternative variations and step sequences, unless expressly provided otherwise. In addition, except in any operating examples, or when otherwise indicated, all numerals representing the amount of the components used in the specification and claims should be understood to be modified by the term "about" in all cases. Therefore, unless otherwise indicated, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary according to the desired performance to be obtained by the present invention. At least, it is not intended to limit the application of the doctrine of equivalents to the scope of the claims, and each numerical parameter should at least be interpreted according to the number of reported significant figures and by applying ordinary rounding techniques.

[0019] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0020] Furthermore, it should be understood that any numerical range recited herein is intended to include all subranges subsumed therein. For example, a range of "1 to 10" is intended to include all subranges between (and including) the recited minimum value of 1 and the recited maximum value of 10, i.e., having a minimum value equal to or greater than 1 and a maximum value equal to or less than 10.

[0021] Example 1

[0022] A method for relieving dormancy of rice seeds comprises the following steps:

[0023] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0024] 2) soaking the rice seeds selected in step 1) in a 0.2% potassium nitrate solution for 24 hours and then washing with clean water;

[0025] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0026] Example 2

[0027] A method for relieving dormancy of rice seeds comprises the following steps:

[0028] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0029] 2) soaking the rice seeds selected in step 1) in a 3% hydrogen peroxide solution for 24 hours and then washing with clean water;

[0030] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0031] Example 3

[0032] A method for relieving dormancy of rice seeds comprises the following steps:

[0033] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0034] 2) soaking the rice seeds selected in step 1) in a 0.1% gibberellin solution for 24 hours and then washing with clean water;

[0035] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0036] Example 4

[0037] A method for relieving dormancy of rice seeds comprises the following steps:

[0038] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0039] 2) soaking the rice seeds selected in step 1) in a mixture of 0.2% potassium nitrate solution and 3% hydrogen peroxide solution for 24 hours, and then washing with clean water;

[0040] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0041] Example 5

[0042] A method for relieving dormancy of rice seeds comprises the following steps:

[0043] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0044] 2) soaking the rice seeds selected in step 1) in a mixture of 0.2% potassium nitrate solution and 0.1% gibberellin solution for 24 hours, and then washing with clean water;

[0045] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0046] Example 6

[0047] A method for relieving dormancy of rice seeds comprises the following steps:

[0048] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0049] 2) soaking the rice seeds selected in step 1) in a mixture of 3% hydrogen peroxide solution and 0.1% gibberellin solution for 24 hours, and then washing with clean water;

[0050] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0051] Example 7

[0052] A method for relieving dormancy of rice seeds comprises the following steps:

[0053] 1) Seed selection: Select dormant rice seeds stored in seed cellars at 4°C;

[0054] 2) soaking the rice seeds selected in step 1) in a mixture of 0.2% potassium nitrate solution, 3% hydrogen peroxide solution, and 0.1% gibberellin solution for 24 hours, and then washing with clean water;

[0055] 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination status.

[0056] The following is a comparison of several methods for relieving rice seed dormancy:

[0057] (1) Potassium nitrate solution treatment: Soak the seeds in 0.2% potassium nitrate solution for 24 hours, wash with clean water, and conduct a standard germination experiment at a constant temperature of 28°C to observe and record the germination situation.

[0058] (2) Treatment with hydrogen peroxide solution: Soak the seeds in 3% hydrogen peroxide solution for 24 hours, wash with clean water, and conduct a standard germination experiment at a constant temperature of 28°C to observe and record the germination situation.

[0059] (3) Gibberellic acid solution treatment: Soak the seeds in 0.1% gibberellin for 24 hours, wash them with clean water, and conduct a standard germination experiment at a constant temperature of 28°C to observe and record the germination situation.

[0060] (4) Treatment with a mixed solution of potassium nitrate and hydrogen peroxide: Soak the seeds in a mixed solution of 0.2% potassium nitrate solution and 3% hydrogen peroxide solution for 24 hours. After washing with clean water, perform a standard germination experiment at a constant temperature of 28°C, and observe and record the germination situation.

[0061] (5) Treatment with a mixed solution of potassium nitrate and gibberellin: Soak the seeds in a mixed solution of 0.2% potassium nitrate solution and 0.1% gibberellin solution for 24 hours. After washing with clean water, conduct a standard germination experiment at a constant temperature of 28°C, and observe and record the germination situation.

[0062] (6) Treatment with hydrogen peroxide + gibberellin solution: Soak the seeds in 3% hydrogen peroxide solution and 0.1% gibberellin solution for 24 hours, wash with clean water, and conduct a standard germination experiment at a constant temperature of 28°C to observe and record the germination situation.

[0063] (7) Treatment with potassium nitrate + hydrogen peroxide + gibberellin solution: Soak the seeds in a mixed solution of 0.2% potassium nitrate solution, 3% hydrogen peroxide solution, and 0.1% gibberellin solution for 24 hours. After washing with clean water, conduct a standard germination experiment at a constant temperature of 28°C, and observe and record the germination situation.

[0064] (8) Heating and drying treatment: Place the seeds in a constant temperature box at 45℃ for drying, spread the seeds into a thin layer, and conduct a standard germination experiment at a constant temperature of 28℃ after 5 days, observe and record the germination situation.

[0065] (9) Control group: Soak seeds in clean water, conduct a standard germination experiment at a constant temperature of 28°C, and observe and record the germination situation.

[0066] The dormant rice variety CO39 was subjected to a germination experiment using the nine treatments described above. Seeds were placed on moistened filter paper in a Petri dish and subjected to a standard germination experiment in a constant-temperature incubator set at (30±1)°C. Each germination box contained 50 seeds, and the number of seeds germinating was recorded daily. After eight days, the germination rate of each variety under each treatment was statistically analyzed. The effects of the nine dormancy-releasing methods on rice seed germination rates are shown in Table 1.

[0067] Table 1

[0068]

[0069]

[0070] As can be seen from the above table, the germination rate of rice seeds treated with potassium nitrate solution, gibberellin solution, potassium nitrate + gibberellin mixed solution, hydrogen peroxide + gibberellin solution, and potassium nitrate + hydrogen peroxide + gibberellin solution was effectively improved compared with the control treatment group in the prior art. The highest germination rates were achieved in the potassium nitrate + hydrogen peroxide + gibberellin solution treatment and the potassium nitrate solution treatment.

[0071] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for releasing rice seed dormancy, characterized in that: The specific steps include: 1) Seed selection: Select rice seeds that are dormant and stored in seed cellars at 4°C; 2) soaking the rice seeds selected in step 1) in a dormancy-release promoting agent for 24 hours and then washing them with clean water; The dormancy-release promoting agent is at least one of 0.2% potassium nitrate solution, 3% hydrogen peroxide solution, and 0.1% gibberellin solution; 3) Conduct a standard germination experiment at a constant temperature of 28°C and observe and record the germination conditions.

2. A method for relieving rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is 0.2% potassium nitrate solution.

3. A method for relieving rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a 3% hydrogen peroxide solution.

4. A method for releasing rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a 0.1% gibberellin solution.

5. A method for releasing rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution and 3% hydrogen peroxide solution.

6. A method for relieving rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution and 0.1% gibberellin solution.

7. A method for releasing rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a mixed solution of 3% hydrogen peroxide solution and 0.1% gibberellin solution.

8. A method for releasing rice seed dormancy according to claim 1, characterized in that: The dormancy-release promoting agent in step 2) is a mixed solution of 0.2% potassium nitrate solution, 3% hydrogen peroxide solution and 0.1% gibberellin solution.

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