Seed storage method of a mangrove plant bruguiera gymnorrhiza

Abstract

The application discloses a seed preservation method of mangrove Bruguiera gymnorrhiza, which comprises three steps of seed pretreatment, seed preservation and seed germination. Specifically, the seeds of the Bruguiera gymnorrhiza are washed and disinfected, dried, and then the water content of the seeds is adjusted to 90%, after which vaseline is applied on the surface of the seeds, the seeds are placed in sand with a humidity of about 60%, and the seeds and the sand are placed in a double-layer plastic bag for sealing and stored in a 17 DEG C environment in the dark. The germination rate of the seeds preserved by the method can reach 100% after 6 months, and the timely and rapid reseeding of the seeds of the Bruguiera gymnorrhiza can be realized.

Description

Technical Field

[0001] This invention belongs to the field of plant seed preservation technology, specifically relating to a method for preserving the seeds of the mangrove plant Rhizoma Rhizoma Cypripedii. Background Technology

[0002] Olive ( Bruguiera gymnorhiza Mangroves (Rhizophora stylosa) are viviparous mangrove plants belonging to the genus Rhizophoraceae in the family Rhizophoraceae. They are widely distributed in my country and are one of the dominant mangrove species. Mangroves not only act as "coastal guardians," protecting against wind and waves and purifying seawater, but also provide habitats for numerous organisms and function as "sea forests" with carbon sequestration capabilities. However, in the past half-century, coastal development, drainage, reclamation, aquaculture, and environmental pollution have continuously emerged in mangrove areas. Due to the severe interference of human activities, the global mangrove area has sharply declined. Therefore, mangrove afforestation is urgently needed.

[0003] The seeds of *Rhizophora stylosa* are spindle-shaped, dark green, 14-20 cm long, and 1.4-1.8 cm wide, with each seed weighing 15-30 g fresh. After harvesting, *Rhizophora stylosa* seeds are susceptible to insect infestation, rotting, and natural dehydration, leading to loss of viability. Planting using *Rhizophora stylosa* seeds is often affected by weather and tides, preventing timely planting. Furthermore, the survival rate after planting is very low due to harsh habitat conditions, frequently requiring replanting. The difficulty in preserving *Rhizophora stylosa* seeds limits afforestation efforts; therefore, finding a long-term preservation method for *Rhizophora stylosa* seeds is crucial for the ecological restoration of *Rhizophora stylosa* mangrove forests.

[0004] Currently, there are no relevant methods for preserving Rhizophora stylosa seeds. This patent extends the survival time of Rhizophora stylosa seeds by controlling the seed moisture content, applying a preservative, sealing them in sand, and finally storing them at a low temperature in the dark, thus enabling timely and rapid replanting of Rhizophora stylosa seeds. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preserving the seeds of the mangrove plant *Rhizophora stylosa*. By controlling the seed moisture content and the amount of moisture loss during preservation, the germination of *Rhizophora stylosa* seeds is freed from dependence on the seed maturity period, enabling the planting of *Rhizophora stylosa* at different times.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] A method for preserving the seeds of the mangrove plant Rhizoma Rhizoma Cypriniforme, comprising the following steps:

[0008] (1) Seed pretreatment: After washing the seeds, disinfect them with carbendazim solution for 15 minutes, wipe the surface moisture of the seeds dry, and select seeds that are uniform in shape, free from obvious diseases and pests, relatively thick and long, and dark green in color.

[0009] (2) Seed preservation: the Bruguiera gymnorrhiza seeds obtained in step (1) are adjusted to have a moisture content of 90%, a layer of vaseline is applied on the surface, and then the Bruguiera gymnorrhiza seeds are placed in sand and put into a double-layer plastic bag for light-proof storage;

[0010] (3) Seed germination: the Bruguiera gymnorrhiza seeds in step (2) are planted in a seedling bag filled with sand and mud mixture, the root of the Bruguiera gymnorrhiza seed is inserted into the seedling bag to a depth of half the length of the Bruguiera gymnorrhiza seed, and a space of 2 cm is left between the bottom of the seedling bag and the bottom of the seed; after watering with clean water, the seedling bag is moved to a germination area, and the seed is allowed to germinate.

[0011] The carbendazim solution in step (1) is prepared by dissolving carbendazim powder with an effective component of 50% in water at a ratio of 1 g:500 mL, and the water temperature is 25°C.

[0012] The method for adjusting the moisture content in step (2) is to place the Bruguiera gymnorrhiza seeds in an oven for 12 hours, and the temperature of the oven is 40°C.

[0013] The humidity of the sand in step (2) is 60%.

[0014] The temperature for light-proof storage in step (2) is 17°C.

[0015] The content of sand in the sand and mud mixture in step (3) is 50%.

[0016] The light intensity of the germination area in step (3) is 120 μmol·m -2 ·s -1 per square meter, the light illumination time is 12 hours per day, the environmental temperature is 26°C, the relative humidity is 80%, and the evaporated water is supplemented every day to keep the seed growth environment moist. The substrate used is a sand and mud mixture, and the content of sand is 50%.

[0017] Compared with the prior art, the present application has the following advantages:

[0018] The present application sets different temperatures, preservatives and moisture contents, and breaks the dependence of Bruguiera gymnorrhiza seed germination on the seed maturation period. The germination rate of the Bruguiera gymnorrhiza seeds preserved by the method can still reach 100% after 6 months, and the Bruguiera gymnorrhiza seeds can be planted in different periods. DETAILED DESCRIPTION

[0019] The technical solutions of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0020] The carbendazim powder in the following examples is Kunning Wangpaimulti-carbendazim from Liaoning Yingkou Leike Pesticide Co., Ltd.; the mud is from the beach land in Jinjiang City, Quanzhou City, Fujian Province. The seed pretreatment step is to disinfect the Bruguiera gymnorrhiza seeds with a carbendazim solution for 15 minutes after washing, and the carbendazim solution is prepared by dissolving 1 g of carbendazim powder with an effective component of 50% in 500 mL of water at a temperature of 25°C. Then the surface moisture of the Bruguiera gymnorrhiza seeds is wiped dry, and the Bruguiera gymnorrhiza seeds with uniform morphology, no obvious pests and diseases, and a dark green color are selected.

[0021] Example 1: Effect of different water contents on Bruguiera gymnorrhiza seed preservation

[0022] The water content of the Bruguiera gymnorrhiza seeds is set to 100% (fresh seeds), 90% and 80% respectively. The specific steps are as follows: first, weigh the fresh Bruguiera gymnorrhiza seeds, then place the weighed seeds in a 40°C oven for 12 hours, and periodically take out the seeds for weighing. When the weight loss of the seeds is 10%, the water content of the seeds is 90%. Similarly, place the Bruguiera gymnorrhiza seeds in a 40°C oven for 24 hours, and when the weight loss of the seeds is 20%, the water content of the seeds is 80%.

[0023] Other conditions are as follows: the preservation method is sand burial, the sand humidity is 60%, the sand and the above-mentioned Bruguiera gymnorrhiza seeds are placed in a double-layer plastic bag and sealed, the preservation temperature is 9°C, and the preservation is in the dark.

[0024] Every other month, the preserved seeds are taken out for germination experiments. The specific steps of the germination experiment are as follows: the preserved Bruguiera gymnorrhiza seeds are planted in a seedling bag containing a mixture of sand and mud (sand content 50%). The Bruguiera gymnorrhiza seeds are planted with the roots pointing downward into the seedling bag, with a depth of half the seed, and a space of about 2 cm is left between the bottom of the seedling bag and the bottom of the seed. After watering with clean water, the seedling bag is moved to the germination area and the seeds are allowed to germinate. The light intensity during germination is 120 μmol·m -2 ·s -1 , the light duration is 12 h / d, and the lamp tube is ZK-TB18-VE02 / A model produced by Zhongke San'an. The environmental temperature is 26°C, the relative humidity is 80%, and the water loss is replenished every day to maintain a humid environment for the seeds.

[0025] Table 1: Effect of different water contents on the germination rate of Bruguiera gymnorrhiza

[0026]

[0027] As shown in Table 1, when the water content is 90%, the preservation time of Bruguiera gymnorrhiza increases, and when the water content is 90%, the germination rate is still 20% after 1 month of preservation.

[0028] Example 2: Effect of different preservatives on Bruguiera gymnorrhiza seed preservation

[0029] The applied preservatives are shellac and vaseline, and the seed moisture content is 100%. Other conditions: sand burial is used for preservation, the sand humidity is 60%, and the seed moisture content is 100%. The sand and the above Bruguiera gymnorrhiza seeds are placed in a double-layer plastic bag for sealing, and the preservation temperature is 9℃ and the preservation is in the dark. The preserved seeds are taken out every other month for germination experiments, and the experimental steps are the same as in Example 1. The light intensity during germination is 120 μmol·m -2 ·s -1 , the light duration is 12h / d, the lamp tube is ZK-TB18-VE02 / A model produced by Zhike Sanan, the environmental temperature is 26℃, the relative humidity is 80%, and the evaporated water is supplemented every day to maintain the seed growth environment moist.

[0030] Table 2 Influence of different preservatives on the germination rate of Bruguiera gymnorrhiza

[0031]

[0032] As shown in Table 2, when the applied preservatives are vaseline and shellac, the preservation time of Bruguiera gymnorrhiza is greatly increased. The germination rate of Bruguiera gymnorrhiza preserved for 3 months is 20% when vaseline is used, and the germination rate is 40% when shellac is used after 2 months of preservation.

[0033] Example 3 Influence of different temperatures on the preservation of Bruguiera gymnorrhiza seeds

[0034] The preservation temperature is set to 4℃, 9℃, 14℃ and 17℃, and other conditions: sand burial is used for preservation, the sand humidity is 60%, and the sand and the above Bruguiera gymnorrhiza seeds are placed in a double-layer plastic bag for sealing and preservation in the dark. The preserved seeds are taken out every other month for germination experiments, and the experimental steps are the same as in Example 1. The light intensity during germination is 120 μmol·m -2 ·s -1 , the light duration is 12h / d, the lamp tube is ZK-TB18-VE02 / A model produced by Zhike Sanan, the environmental temperature is 26℃, the relative humidity is 80%, and the evaporated water is supplemented every day to maintain the seed growth environment moist.

[0035] Table 3 Influence of different preservation temperatures on the germination rate of Bruguiera gymnorrhiza

[0036]

[0037] As shown in Table 3, when the preservation temperature is 17℃, the preservation time of Bruguiera gymnorrhiza is greatly increased. The germination rate is 80% when the preservation time is 4 months, but the germination rate is 0% when the preservation time is 5 months and 6 months.

[0038] Example 4 Influence of optimal conditions on the preservation of Bruguiera gymnorrhiza seeds

[0039] The seed surface of Bruguiera gymnorrhiza is smeared with vaseline after the water content of the seed is set to 90%, and the seed is stored in the dark at 17°C. Other conditions: sand burial is used for storage, the humidity of the sand is 60%, the sand and the Bruguiera gymnorrhiza seed are placed in a double-layer plastic bag for sealing, and the seed is stored in the dark. The stored seed is taken out every other month for germination experiment, and the experimental steps are the same as in Example 1. The light intensity during germination is 120 μmol·m -2 ·s -1 , the light duration is 12 h / d, the lamp tube is ZK-TB18-VE02 / A produced by Zhike San'an, the environmental temperature is 26°C, the relative humidity is 80%, and the water loss is supplemented every day to maintain the humidity of the seed growth environment.

[0040] Table 4. Influence of optimal conditions on the germination rate of Bruguiera gymnorrhiza

[0041]

[0042] As shown in Table 4, when the optimal conditions are used, the storage time of Bruguiera gymnorrhiza is greatly increased, and the germination rate is still 100% when the storage time is 6 months.

[0043] In summary, when the water content of Bruguiera gymnorrhiza seed is controlled at 90%, 20% of the seeds can germinate after 1 month of storage (Table 1). The germination rate of Bruguiera gymnorrhiza seed is still 20% after 3 months of storage with vaseline (Table 2). The germination rate of Bruguiera gymnorrhiza seed is still 100% after 6 months of storage at 17°C (Table 3). When the optimal conditions are used, the storage time is up to 6 months, and the germination rate is still 100% (Table 4). Therefore, after the water content of Bruguiera gymnorrhiza seed is adjusted to 90%, the seed is smeared with vaseline, placed in sand with a humidity of about 60%, and the seed and sand are placed in a double-layer plastic bag for sealing, and the seed is stored in the dark at 17°C, which is a suitable storage condition for Bruguiera gymnorrhiza seed.

[0044] The above only describes the preferred embodiments of the present application, and any equivalent changes and modifications made within the scope of the patent application of the present application shall be included in the scope of the present application.

Claims

1. A method for preserving seeds of Bruguiera gymnorrhiza, characterized by: The method comprises the following steps: (1) seed pretreatment: after washing the bruguiera gymnorrhiza seeds, disinfect them with a carbendazim solution for 15 minutes, wipe off the water on the surface of the bruguiera gymnorrhiza seeds, and screen out the bruguiera gymnorrhiza seeds with uniform shape, no obvious pests and diseases, and relatively long and dark green color; (2) seed storage: adjust the moisture content of the bruguiera gymnorrhiza seeds obtained in step (1) to 90%, smear a layer of vaseline on the surface of the bruguiera gymnorrhiza seeds, and then place them in sand, and then put them in a double-layer plastic bag for sealing, and store them in the dark; (3) seed germination: take out the bruguiera gymnorrhiza seeds in step (2) and plant them in a seedling bag filled with sand and mud mixture, the root of the bruguiera gymnorrhiza seed is inserted into the seedling bag, the insertion depth is half of the length of the bruguiera gymnorrhiza seed, the bottom of the seedling bag is 2 cm away from the bottom of the seed, pour clean water, and then move the seedling bag to the germination area, and wait for the seed to germinate; The carbendazim solution in step (1) is a carbendazim powder with an effective component of 50%, which is dissolved in water at a ratio of 1 g:500 mL, and the water temperature is 25°C; The method for adjusting the moisture content in step (2) is to place the bruguiera gymnorrhiza seeds in an oven for 12 hours, and the temperature of the oven is 40°C; The humidity of the sand in step (2) is 60%; The temperature for storing in the dark in step (2) is 17°C; The content of sand in the sand and mud mixture in step (3) is 50%; The content of sand in the sand and mud mixture in step (3) is 50%; The light intensity of the germination zone in step (3) is 120 μmol·m -2 ·s -1 , the light duration is 12 hours per day, the ambient temperature is 26°C, the relative humidity is 80%, and the evaporated water is supplemented every day to maintain the humidity of the seed growth environment.

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