Method for culturing nereis by using artificial perching tube

Abstract

The invention discloses a method for culturing nereis by using an artificial perching tube. The method comprises the following steps: measuring the body size of the nereis, constructing the U-shaped artificial perching tube, putting the nereis into a water pool, culturing and catching. The improved PVC transparent hose and the artificial perching pipe suitable for growth of the nereis are utilized, self-adaption of the perching pipe of the nereis is achieved through the inner ring of the pipe, the nereis adapts to growth in the perching pipe, the growth state and ecological behaviors of the nereis can be observed in a laboratory, manual catching is easy, and the nereis survival rate is increased. The problem that sedimentary substances such as sea mud must be used in polytrichum culture is greatly reduced, and a simple and rapid method is provided for polytrichum culture and scientific experiments.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for cultivating sandworms by using artificial habitat tubes. Background Art

[0002] Nereids belong to the phylum marine annelids, class polychaete, and are marine animals that live at the bottom of marine sediments. The marine sediments where nereids live are mostly mud, sand, or sea mud mixed with gravel and shell fragments. Under normal living conditions, nereids dig holes in the sea mud to form their own caves, and then go in and out of the caves, forming a unique feeding and lifestyle.

[0003] In traditional factory farming or indoor farming, the cultured sandworms require a certain thickness of mud or sandy mud substrate, with a substrate thickness of about 10-20 cm. The existence of the substrate makes it impossible to observe the growth status of the sandworms and to confirm whether the sandworms have grown to a state where they can be harvested. At the same time, manual harvesting is more difficult, and usually requires the use of a special iron rake to rake the soil, and then manually pick out the sandworms one by one. In the process of raking the soil with an iron rake, the sandworms are often damaged by the iron rake, causing problems such as broken body segments. In addition, with the substantial increase in labor costs, the cost of harvesting has increased rapidly.

[0004] At the same time, the ecological behavior of sandworms needs to be observed during the laboratory culture process. At present, most reports on the breeding of sandworms focus on various different methods of breeding. For example, in 2023, Huang Yongqiang et al. reported the breeding method of warty sandworms in ponds in the ecological breeding experiment of warty sandworms; in 2022, Zhang Bin et al. reported the facility breeding device of sandworms based on plant matrix in the facility breeding device of sandworms based on plant matrix and sandworm breeding method; in 2021, Luo Huaming et al. reported the method of culturing sandworms in cement pools in the sandworm cement pool breeding experiment. These are all breeding methods with various substrates or substrates. In addition, in 2012, Yang Wei et al. discussed the breeding experiment of sandworm parents with Gracilaria as the substrate in the gonad development of double-toothed peri-nereid cultured in the habitat of Gracilaria. In 2010, Yang Wei reported the cultivation of sandworm parents under soilless culture conditions in the effect of temperature on the cultivation of double-toothed peri-nereid parents under soilless culture conditions. Although it is clearly soilless, its substrate is actually Gracilaria. All of the above reports use plants as the substrate for their cultivation. Although the sandworms do not burrow into the soil, they still drill holes in the plants, making them difficult to capture and observe. Summary of the invention

[0005] The present invention provides a method for cultivating sandworms by utilizing artificial habitat tubes, which innovates the soilless cultivation of sandworms and forms a new cultivation method which is easy to observe the growth state and ecological behavior of sandworms and easy to capture.

[0006] The technical solution adopted by the present invention is: A method for cultivating sandworms using an artificial habitat tube comprises the following steps: S1. Determine the body size of the lugworm; S2. Constructing a U-shaped artificial habitat: bend the PVC transparent hose and tie its two ends with a string or rubber band to form a U-shaped artificial habitat; S3, placing the sandworm into the U-shaped artificial habitat tube constructed in S2, and then placing it in a pool; S4, breeding: the artificial habitat tubes containing sandworms obtained in S3 are placed side by side in a breeding pool, oxygen is introduced into the pool using an air pump, and feed is placed at the mouth of the artificial habitat tube to ensure that the sandworms can feed and grow normally.

[0007] Preferably, in S1, the nereid to be raised is placed in seawater containing alcohol, the body of the nereid is fully relaxed, the water on the body surface is absorbed by filter paper, and then the maximum body length is measured; Take another sandworm of the same size and put it into seawater containing alcohol. The sandworm's body will shrink completely. After the water on the body surface is absorbed by filter paper, its maximum body width will be measured.

[0008] Preferably, in S1, the sandworm is placed in seawater containing 5%-10% alcohol to allow its body to fully relax.

[0009] Preferably, in S1, the sandworm is placed in seawater containing 70%-75% alcohol to completely shrink its body.

[0010] Preferably, in S2, the artificial habitat is a PVC transparent hose.

[0011] Preferably, a plurality of inner rings are provided in the artificial habitat, and a small hole is provided in the middle portion above the inner rings.

[0012] Preferably, the inner diameter of the artificial habitat is 1.5 times the maximum body width of the sandworm measured in S1, and its length is 2 times the maximum body length of the sandworm measured in S1.

[0013] Preferably, the inner ring of the tube in S2 is a foamed polyethylene cylinder; The length of the inner ring of the tube is 0.5-1 cm, and its diameter is equal to the inner diameter of the artificial habitat tube; The diameter of the small hole inside the inner ring of the tube is equal to the maximum body width of the sandworm measured in S1, and the length of the inner hole is equal to the length of the inner ring of the tube.

[0014] Preferably, a first inner tube ring, a second inner tube ring and / or a third inner tube are sequentially placed on the artificial habitat tube from left to right; The second inner ring is arranged in the middle of the artificial habitat tube, and the first inner ring and / or the third inner tube are 0.5-1 cm away from one end of the artificial habitat tube.

[0015] Preferably, the present invention also includes the capture of sandworms, and the specific operation is: observe the growth of sandworms outside the habitat tube, and when the sandworms grow up and need to be captured, use a small hook to take out the inner ring at one end of the artificial habitat tube, untie the thin rope or rubber band that binds the artificial habitat tube to restore it to a straight shape, and pour the sandworms directly out of the tube.

[0016] In summary, the technical solution of the present invention has the following beneficial effects: The present invention utilizes a modified transparent plastic hose to artificially simulate an artificial habitat tube suitable for the growth of lugworms, utilizes an inner ring of the tube to achieve self-adaptation of the lugworms, enables the lugworms to adapt to growing in the habitat tube, facilitates laboratory observation of the growth status and ecological behavior of the lugworms, and facilitates artificial capture, greatly reducing the problem of having to use sediments such as sea mud in polychaete breeding, and provides a simple and quick method for polychaete breeding and scientific experiments.

[0017] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Schematic diagram of the U-shaped artificial habitat provided in Example 1 and Example 2. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0020] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0021] Example 1 A method for culturing Perineuri bilaterians using an artificial habitat tube comprises the following steps: S1. Determine the body size of sandworms: Place the dicynodontids to be cultured in a dish containing 10% alcohol in seawater to allow their bodies to relax. After taking them out, use filter paper to absorb the moisture on the body surface and use a ruler to measure their maximum body length (L).

[0022] Take a double-toothed perinectin of the same size and specification and put it into another dish containing 75% alcohol on the surface of seawater. After its body is anesthetized and shrinks, take it out, use filter paper to absorb the moisture on the body surface, and use a ruler to measure its maximum body width (W).

[0023] S2. Construct a U-shaped artificial habitat: The artificial habitat is a PVC transparent hose, the inner diameter (D) of the artificial habitat is 1.5 times the maximum body width (W) of the nereid measured in S1, and its length (C) is 2 times the maximum body length (L) of the nereid measured in S1; An inner ring is provided in the artificial habitat tube, and a small hole is provided in the middle above the inner ring. The hole diameter (K) is equal to the maximum body width of the sandworm measured in S1 (W), and the inner hole length is 0.5 cm.

[0024] The inner ring of the tube is a foam polyethylene cylinder with a length of 0.5 cm and a diameter equal to the inner diameter of the artificial habitat tube (D); The first inner ring, the second inner ring and the third inner ring are placed in the artificial habitat from left to right in sequence; wherein, the second inner ring is arranged in the middle of the artificial habitat, and the first inner ring and the third inner ring are 0.8 cm away from one end of the artificial habitat.

[0025] Bend the artificial habitat tube and tie its two ends with thin ropes to form a U-shaped artificial habitat tube, similar to the cave of the double-toothed sandworm.

[0026] S3, placing the bidentate perineri into the U-shaped artificial habitat tube constructed in S2, and then placing it in a pool; S4. Breeding: 100 artificial habitat tubes containing double-toothed perinectines are placed side by side in a breeding pool. Oxygen is introduced into the pool using an air pump, and feed is placed at the mouth of the artificial habitat tube to ensure that the necrophiles can feed and grow normally.

[0027] This embodiment also includes the capture of double-toothed sandworms. The specific operation is: observe the growth of double-toothed sandworms outside the habitat tube. When the sandworms grow up and need to be captured, use a small hook to take out the inner ring at one end of the artificial habitat tube, untie the thin rope that binds the artificial habitat tube to restore it to a straight shape, and pour the sandworms directly out of the tube.

[0028] Example 2 A method for cultivating Japanese spiny worms using an artificial habitat tube comprises the following steps: S1. Determine the body size of the sandworm: Place the Japanese sandworm to be cultured in a dish containing 5% alcohol on the surface of seawater to allow its body to relax. Take it out and use filter paper to absorb the moisture on the body surface. Use a ruler to measure its maximum body length (L).

[0029] Take Japanese spiny sandworms of the same size and specification and put them into another dish containing 70% alcohol on the surface of seawater. After their bodies are anesthetized and shrink, take them out and use filter paper to absorb the moisture on the surface of their bodies. Use a ruler to measure their maximum body width (W).

[0030] S2. Construct a U-shaped artificial habitat: The artificial habitat is a PVC transparent hose, the inner diameter (D) of the artificial habitat is 1.5 times the maximum body width (W) of the nereid measured in S1, and its length (C) is 2 times the maximum body length (L) of the nereid measured in S1; An inner ring is provided in the artificial habitat tube, and a small hole is provided in the middle above the inner ring. The hole diameter (K) is equal to the maximum body width of the sandworm measured in S1 (W), and the inner hole length is 0.5 cm.

[0031] The inner ring of the tube is a foam polyethylene cylinder with a length of 0.5 cm and a diameter equal to the inner diameter of the artificial habitat tube (D); The first inner ring, the second inner ring and the third inner ring are placed in the artificial habitat from left to right in sequence; wherein, the second inner ring is arranged in the middle of the artificial habitat, and the first inner ring and the third inner ring are 0.5 cm away from one end of the artificial habitat.

[0032] Bend the artificial habitat tube and tie its two ends with rubber bands to form a U-shaped artificial habitat tube, similar to the cave of Japanese spiny sandworm.

[0033] S3, placing the Japanese spiny sandworm into the U-shaped artificial habitat tube constructed in S2, and then placing it in a pool; S4. Breeding: 150 artificial habitats containing Japanese spiny sandworms are placed side by side in a breeding pool. Oxygen is introduced into the pool using an air pump, and feed is placed at the mouth of the artificial habitat to ensure that the sandworms can feed and grow normally.

[0034] This embodiment also includes the capture of Japanese spiny sandworms. The specific operation is: observe the growth of Japanese spiny sandworms outside the habitat tube. When the sandworms grow up and need to be captured, use a small hook to take out the inner ring at one end of the artificial habitat tube, untie the rubber band that binds the artificial habitat tube to restore it to a straight shape, and pour the sandworms directly out of the tube.

[0035] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for cultivating sandworms using artificial habitat tubes, characterized in that: The steps include: S1. Determine the body size of the lugworm; S2. Constructing a U-shaped artificial habitat: bend the PVC transparent hose and tie its two ends with a string or rubber band to form a U-shaped artificial habitat; S3, placing the sandworm into the U-shaped artificial habitat tube constructed in S2, and then placing it in a pool; S4, breeding: the artificial habitat tubes containing sandworms obtained in S3 are placed side by side in a breeding pool, oxygen is introduced into the pool using an air pump, and feed is placed at the mouth of the artificial habitat tube to ensure that the sandworms can feed and grow normally.

2. A method for cultivating lugworms using artificial habitats according to claim 1, characterized in that: In S1, the nereids to be cultured are placed in seawater containing alcohol, the bodies of the nereids are fully relaxed, the water on the body surface is absorbed by filter paper, and then the maximum body length is measured; Take another sandworm of the same size and put it into seawater containing alcohol. The sandworm's body will shrink completely. After the water on the body surface is absorbed by filter paper, its maximum body width will be measured.

3. A method for cultivating lugworms using artificial habitat tubes according to claim 2, characterized in that: In S1, the sandworms were placed in seawater containing 5%-10% alcohol to allow their bodies to fully relax.

4. A method for cultivating lugworms using artificial habitat tubes according to claim 2, characterized in that: In S1, the sandworms were placed in seawater containing 70%-75% alcohol to allow their bodies to completely shrink.

5. The method for cultivating lugworms using artificial habitat tubes according to claim 1, characterized in that: In S2, the artificial habitat is a PVC transparent hose.

6. A method for cultivating lugworms using artificial habitats according to claim 5, characterized in that: A plurality of inner tube rings are arranged in the artificial habitat tube, and a small hole is arranged in the middle part above the inner tube rings.

7. A method for cultivating lugworms using artificial habitats according to claim 6, characterized in that: The inner diameter of the artificial habitat is 1.5 times the maximum body width of the sandworm measured in S1, and its length is twice the maximum body length of the sandworm measured in S1.

8. The method for cultivating lugworms using artificial habitat tubes according to claim 7, characterized in that: The inner ring of the S2 tube is a foam polyethylene cylinder; The length of the inner ring of the tube is 0.5-1 cm, and its diameter is equal to the inner diameter of the artificial habitat tube; The diameter of the small hole inside the inner ring of the tube is equal to the maximum body width of the sandworm measured in S1, and the length of the inner hole is equal to the length of the inner ring of the tube.

9. The method for cultivating lugworms using artificial habitat tubes according to claim 6, characterized in that: The first tube inner ring, the second tube inner ring and / or the third tube inner tube are placed on the artificial habitat tube from left to right in sequence; The second inner ring is arranged in the middle of the artificial habitat tube, and the first inner ring and / or the third inner tube are 0.5-1 cm away from one end of the artificial habitat tube.

10. The method for cultivating lugworms using artificial habitat tubes according to claim 1, characterized in that: It also includes the capture of sandworms, and the specific operation is: observe the growth of sandworms outside the habitat tube, and when the sandworms grow up and need to be captured, use a small hook to take out the inner ring at one end of the artificial habitat tube, untie the thin rope or rubber band that binds the artificial habitat tube to restore it to a straight shape, and pour the sandworms directly out of the tube.

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