Method for planting northern arid desert plants in southern wet desert and application of northern arid desert plants

Abstract

The invention discloses a method for planting northern arid desert plants in a southern wet desert and application of the northern arid desert plants in the southern wet desert. The method comprises the following step that the northern arid desert plants and vitex rotundifolia are planted in the southern wet desert together. According to the method provided by the scheme, the northern arid desert plant liquorice can grow in the southern wet desert, and vitex rotundifolia can serve as a symbiotic plant to cool roots of liquorice seedlings and middle seedlings; during raining, vitex rotundifolia guides water through rhizomes to guarantee water replenishment of the sandy soil, and after raining, the water content of the sandy soil is high, so that the vitex rotundifolia can balance the water content of the sandy soil through transpiration; in the case of sunny and dry days, the vitex rotundifolia keeps the sandy soil moist under the action of capillaries, the purpose that the northern arid desert plant liquorice is planted in the southern moist desert for the first time is achieved through the method, and a new treatment approach is provided for vegetation restoration and reconstruction in the desertification land area of the bottomland of the bottomland of the bottomland of the bottomland of the bottomland.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for planting northern arid desert plants in southern humid deserts and an application thereof. Background Art

[0002] Desertification refers to land degradation in arid, semi-arid and sub-humid arid areas caused by various factors including climate change and human activities. Land desertification is a major global environmental problem and a major disaster that threatens human survival and development. The development of desertification has caused biological quality to deteriorate and species abundance to decrease, posing a serious threat to biodiversity. China's desertification is mainly divided into four types: wind erosion desertification, water erosion desertification, freeze-thaw desertification, and soil salinization. Among them, the sand dunes around Poyang Lake in Jiangxi Province are a typical type of desertification in the south. They are formed by wind erosion and water erosion on the basis of lakeside sandy terraces. They are different from land desertification in the north and other types of desertification in the south. The sand dunes around Poyang Lake not only threaten the production and life of local residents, but also affect the flood discharge of Poyang Lake and the smooth flow of waterways, seriously hindering the sustainable development of the local economy and ecology.

[0003] Nitrogen (N) is the main limiting factor for plant growth in terrestrial ecosystems. Free nitrogen in the air accounts for about 79% of the air composition. However, most plants cannot directly utilize nitrogen in the air and must rely on absorbing bound nitrogen from the soil to meet their own needs. Biological nitrogen fixation (BNF) is driven by a highly diverse microbial community, based on the enzymatic "nitrogenase" that converts atmospheric nitrogen (N2) into ammonium that is easily absorbed by the roots. As an important component of the soil, microorganisms play an indispensable role in nutrient acquisition, nitrogen cycle, carbon cycle, soil morphogenesis, mineral absorption, and enhancing plant tolerance to various environmental stresses. They are an important indicator for evaluating soil texture. Microbial diversity plays a vital role in soil ecosystems and is an important aspect of defining soil health and soil ecological stability.

[0004] Therefore, as far as the Poyang Lake sand dunes are concerned, microorganisms are the drivers of key processes in the nitrogen cycle and are of great significance to the restoration and reconstruction of vegetation in the desertified land areas of Poyang Lake. There is an urgent need to provide a microbial composition that can be used to repair the sand dunes around Poyang Lake. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a method for planting northern arid desert plants in southern humid deserts.

[0006] The present invention also proposes the application of the above method.

[0007] The present invention also provides a microbial composition.

[0008] The present invention also provides a method for preparing the microbial composition.

[0009] The present invention also provides the application of the above microbial composition.

[0010] According to a first aspect of the present invention, a method for planting northern arid desert plants in southern humid deserts is provided, comprising the following steps: planting northern arid desert plants together with Vitex simplicifolia in southern humid deserts.

[0011] In some embodiments of the present invention, the planting distance between the northern arid desert plant and Vitex simplicifolia is 50-70 cm.

[0012] In some embodiments of the present invention, the planting pattern of the northern arid desert plant and Vitex simplicifolia includes a surrounding planting type.

[0013] In some embodiments of the present invention, the method further comprises the step of applying the microbial composition to the boreal arid desert plants.

[0014] In some embodiments of the present invention, the microbial composition comprises cyanobacteria and actinomycetes, and the addition ratio of the cyanobacteria to the actinomycetes is (1-2): (1-3).

[0015] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 3×10 9 / mL-8×10 9 / mL.

[0016] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 4×10 9 / mL-6×10 9 / mL.

[0017] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 5×10 9 / mL.

[0018] In some embodiments of the present invention, the application amount of the microbial composition is 300-800 mL / plant.

[0019] In some embodiments of the present invention, the application amount of the microbial composition is 400-600 mL / plant.

[0020] In some embodiments of the present invention, the southern humid desert includes the sand dunes around Poyang Lake.

[0021] In some embodiments of the invention, the boreal desert plant comprises licorice.

[0022] In some embodiments of the present invention, the licorice includes Glycyrrhiza uralensis, Glycyrrhiza inflata and Glycyrrhiza glabra.

[0023] In some embodiments of the present invention, the northern desert plants are planted using lateral roots.

[0024] In some embodiments of the present invention, the length of the lateral roots is 10-15 cm.

[0025] In some embodiments of the present invention, the method further comprises the step of sterilizing the lateral roots.

[0026] In some embodiments of the present invention, the disinfectant used for disinfection includes carbendazim.

[0027] In some embodiments of the present invention, the disinfection time is 3-7 minutes.

[0028] In some embodiments of the present invention, the method further comprises treating the disinfected lateral roots with rooting powder.

[0029] In some embodiments of the present invention, the treatment time is 1.5-2.5 hours.

[0030] In some embodiments of the present invention, the planting depth is 10-30 cm.

[0031] In some embodiments of the present invention, the height of the Vitex simplicifolia is 5-25 cm.

[0032] In some embodiments of the present invention, the planting density of the simple leaf vitex is 50-85 plants / m 2 .

[0033] In some embodiments of the present invention, the planting density of the simple leaf vitex is 58-81.2 plants / m 2 .

[0034] In some embodiments of the present invention, the planting also includes the step of field management, which includes field prevention and control when pests and diseases are present, sterilization with pesticides during high temperature and high humidity periods, and weeding and fertilization management steps.

[0035] In some embodiments of the present invention, the high temperature and high humidity period is from July to September.

[0036] In some embodiments of the invention, the fungicide comprises carbendazim.

[0037] In some embodiments of the present invention, the fertilization management includes the step of applying phosphate fertilizer or compound fertilizer 1 to 2 times when the seedling height is about 10 to 15 cm and before rainy days, each time at 8-12 kg / mu.

[0038] According to a second aspect of the present invention, an application of the above method of planting northern arid desert plants in southern humid deserts is proposed, and the application is an application in restoring southern humid deserts.

[0039] In some embodiments of the present invention, the southern humid desert includes the sand dunes around Poyang Lake.

[0040] In some embodiments of the invention, the boreal desert plant comprises licorice.

[0041] In some embodiments of the present invention, the licorice includes Glycyrrhiza uralensis, Glycyrrhiza inflata and Glycyrrhiza glabra.

[0042] According to a third aspect of the present invention, a microbial composition is provided, wherein the microbial composition comprises cyanobacteria and actinomycetes, wherein the cyanobacteria and actinomycetes are added in a ratio of (1-2): (1-3).

[0043] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 3×10 9 / mL-8×10 9 / mL.

[0044] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 4×10 9 / mL-6×10 9 / mL.

[0045] In some embodiments of the present invention, the concentration of microorganisms in the microbial composition is 5×10 9 / mL.

[0046] According to a fourth aspect of the present invention, a method for preparing the above-mentioned composition is proposed, and the method comprises the following steps: mixing cyanobacteria and actinomycetes to obtain.

[0047] According to a fifth aspect of the present invention, the use of the above-mentioned microbial composition in any of the following is proposed:

[0048] (1) Improve the survival rate of plants;

[0049] (2) Promote plant growth;

[0050] (3) Increase the number of plant leaves;

[0051] (4) Restoration of the southern humid desert;

[0052] (5) Prepare reagents for repairing southern humid deserts.

[0053] In some embodiments of the invention, the plant comprises a boreal desert plant.

[0054] In some embodiments of the present invention, the plants include plants transplanted from northern deserts to grow in southern humid deserts.

[0055] In some embodiments of the invention, the boreal desert plant comprises licorice.

[0056] In some embodiments of the present invention, the licorice includes Glycyrrhiza uralensis, Glycyrrhiza inflata and Glycyrrhiza glabra.

[0057] In some embodiments of the present invention, the southern humid desert includes the sand dunes around Poyang Lake.

[0058] According to some embodiments of the present invention, at least the following beneficial effects are achieved: the scheme of the present invention provides a method for planting northern arid desert plants in southern humid deserts. The method of the present invention can be used to plant licorice, a northern arid desert plant, in southern humid deserts for the first time. The method allows licorice, a northern arid desert plant, to grow in southern humid deserts by planting licorice and Vitex unifolia together in southern humid deserts, wherein Vitex unifolia can be used as a symbiotic plant to cool the roots of licorice seedlings and mid-sized seedlings; when it rains, Vitex unifolia conducts water through its rhizomes to ensure water replenishment in the sand. After the rain, the sand has a high water content, and Vitex unifolia can balance the water content of the sand through transpiration; when it is clear and dry in the evening, Vitex unifolia keeps the sand moist through capillary action. At the same time, the method of the present invention also adds a microbial composition, which can effectively improve the nutrients and microenvironment of the sand, and improve the survival rate of licorice planted in southern humid deserts, providing a new governance approach for vegetation restoration and reconstruction in the desertified land area of ​​Poyang Lake.

[0059] The scheme of the present invention also screens and obtains the microbial composition of the scheme of the present invention by analyzing the physical and chemical properties, soil enzyme activity and microbial community of the soil of Vitex unifolia in the Poyang Lake Sand Hill; the microbial composition prepared by the scheme of the present invention can effectively improve the survival rate of the northern arid desert plant licorice planted in the southern humid desert (Poyang Lake Sand Hill), and can also effectively promote the growth of licorice and increase the number of licorice leaves. It has practical guiding significance for the ecological restoration of southern humid sandy land, and provides a governance approach for soil microorganisms to restore and rebuild vegetation in the Poyang Lake desertified land area. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0061] Figure 1 The figure is a test result diagram of the physical and chemical properties of soil at different depths in the habitat of single-leaf vitex in area B of Example 2 of the present invention, wherein Figure A is a pH value test result diagram; Figure B is a soil moisture content test result diagram; Figure C is a ground temperature test result diagram; Figure D is a ground humidity test result diagram; Figure E is an underground temperature test result diagram; lowercase letters a, b, c, d, e, ab, abc, bc indicate significant differences between treatments P<0.05; exp. is where vitex grows in area B, and con. is where vitex does not grow in area B;

[0062] Figure 2 It is a graph showing the test results of the physical and chemical properties of the soil of Vitex unifolia in different planting areas in Example 2 of the present invention, wherein Figure A is a graph showing the test results of the total nitrogen content; Figure B is a graph showing the test results of the alkaline nitrogen content; Figure C is a graph showing the test results of the urease activity; Figure D is a graph showing the test results of the nitrogenase content; lowercase letters a, b, c, d, and e indicate that there are significant differences between the treatments P<0.05; LCK is the blank control of the A test area, XCK is the blank control of the B test area, LGZ is the root soil of the A test area, XGZ is the root soil of the B test area, LGJ is the rhizosphere soil of the A test area, and XGJ is the rhizosphere soil of the B test area;

[0063] Figure 3 It is a composition and relative abundance detection result diagram of soil bacteria community at the phylum level in different groups in Example 2 of the present invention, wherein Figure A is a dominant population and proportion diagram of soil bacteria in the root periphery soil at the phylum level; Figure B is a soil bacteria analysis result diagram; Figure C is a soil bacteria analysis result diagram; Figure D is a soil bacteria analysis result diagram; Figure E is a soil bacteria analysis result diagram; Figure F is a soil bacteria analysis result diagram; Figure G is a soil bacteria analysis result diagram, lowercase letters a, b, c, d, ab, bc, cd indicate that there are significant differences between treatments P<0.05; LCK is the blank control of test area A, XCK is the blank control of test area B, LGZ is the root periphery soil of test area A, XGZ is the root periphery soil of test area B, LGJ is the rhizosphere soil of test area A, and XGJ is the rhizosphere soil of test area B;

[0064] Figure 4 It is a composition and relative abundance detection result diagram of soil bacteria community at genus level in different groups in Example 2 of the present invention, wherein A is a dominant population and proportion diagram of soil bacteria in rhizosphere at genus level; B is a soil bacteria analysis result diagram; C is a soil bacteria analysis result diagram; D is a soil bacteria analysis result diagram; E is a soil bacteria analysis result diagram; F is a soil bacteria analysis result diagram; G is a soil bacteria analysis result diagram, lowercase letters a, b, c, d, ab, bc, cd, abc, bcd indicate significant differences between treatments P<0.05; LCK is blank control of test area A, XCK is blank control of test area B, LGZ is rhizosphere soil of test area A, XGZ is rhizosphere soil of test area B, LGJ is rhizosphere soil of test area A, and XGJ is rhizosphere soil of test area B;

[0065] Figure 5 It is a composition analysis result diagram of soil fungi at the phylum level in Example 2 of the present invention, wherein Figure A is a dominant population and proportion diagram of soil fungi in the peri-root soil at the phylum level; Figure B is a soil fungi analysis result diagram; Figure C is a soil fungi analysis result diagram; Figure D is a soil fungi analysis result diagram; Figure E is a soil fungi analysis result diagram, lowercase letters a, b, c, ab, bc indicate significant differences between treatments P<0.05; LCK is the blank control of the A test area, XCK is the blank control of the B test area, LGZ is the peri-root soil of the A test area, XGZ is the peri-root soil of the B test area, LGJ is the rhizosphere soil of the A test area, and XGJ is the rhizosphere soil of the B test area;

[0066] Figure 6 It is a composition analysis result diagram of soil fungi at the genus level in Example 2 of the present invention, wherein Figure A is a dominant population and proportion diagram of soil fungi in the peri-root soil at the genus level; Figure B is a soil fungi analysis result diagram; Figure C is a soil fungi analysis result diagram; Figure D is a soil fungi analysis result diagram; Figure E is a soil fungi analysis result diagram, lowercase letters a, b, ab indicate that there are significant differences between treatments P<0.05; LCK is the blank control of the A test area, XCK is the blank control of the B test area, LGZ is the peri-root soil of the A test area, XGZ is the peri-root soil of the B test area, LGJ is the rhizosphere soil of the A test area, and XGJ is the rhizosphere soil of the B test area;

[0067] Figure 7 The composition and relative abundance analysis results of the plant endophyte community in different groups in Example 2 of the present invention are shown in Figure A, wherein Figure A is a detection diagram of the bacterial community of the roots and leaves of Vitex unifolia at the phylum level; Figure B is a detection diagram of the bacterial community of the roots and leaves of Vitex unifolia at the genus level; Figure C is a detection result diagram of the fungal community of the roots and leaves of Vitex unifolia at the phylum level; Figure D is a detection result diagram of the fungal community of the roots and leaves of Vitex unifolia at the genus level;

[0068] Figure 8 It is a diagram of the principal coordinate analysis result of the microbial community in Example 2 of the present invention, wherein Figure A is a principal coordinate analysis diagram of the bacterial community of the roots and leaves of single-leaf vitex; Figure B is a principal coordinate analysis diagram of the fungal community of the roots and leaves of single-leaf vitex; Figure C is a principal coordinate analysis diagram of the bacterial community of the roots and leaves of single-leaf vitex; Figure D is a principal coordinate analysis diagram of the fungal community of the roots and leaves of single-leaf vitex, LCK is the blank control of the A test area, XCK is the blank control of the B test area, LGZ is the root soil of the A test area, XGZ is the root soil of the B test area, LGJ is the rhizosphere soil of the A test area, XGJ is the rhizosphere soil of the B test area, LGN is the root, and LYN is the leaf;

[0069] Fig. 9 It is a redundancy analysis result diagram of soil bacteria and fungal communities and soil physical and chemical properties in Example 2 of the present invention; wherein, Figure A is a redundancy analysis result diagram of soil bacteria communities and soil physical and chemical properties; Figure B is a redundancy analysis result diagram of soil fungal communities and soil physical and chemical properties;

[0070] Fig.10 This is a graph showing the LEfSe analysis of bacteria in soils of different groups and roots and leaves of Vitex unifolia in Example 2 of the present invention;

[0071] Fig.11 This is a graph showing the LEfSe analysis of fungi in soils of different groups and roots and leaves of Vitex unifolia in Example 2 of the present invention;

[0072] Fig.12 These are the LEfSe analysis diagrams of bacteria and fungi in different groups of soil and roots and leaves of single-leaf Vitex in Example 2 of the present invention; wherein, Figure C is the LEfSe analysis diagram of bacteria in roots and leaves of single-leaf Vitex; and Figure D is the LEfSe analysis diagram of fungi in roots and leaves of single-leaf Vitex;

[0073] Fig.13 This is a typical growth pattern diagram of licorice in a sand hill in a test example of the present invention;

[0074] Fig.14 This is a result diagram of the effect of single-leaf vitex environment at different positions on the plant height of licorice in the test example of the present invention;

[0075] Fig.15 This is a planting diagram of Shashan Licorice in the test example of the present invention;

[0076] Fig.16 This is a planting analysis diagram of Glycyrrhiza uralensis in the test example of the present invention, wherein lowercase letters a and b indicate significant differences between treatments P<0.05;

[0077] Fig.17 It is a schematic diagram of the growth cycle of the medicinal licorice (Glycyrrhiza spp.) in the test example of the present invention from germination to maturity throughout the season;

[0078] Fig.18 These are statistical results of leaves, plant height, root depth and biomass of medicinal licorice at different growth stages in the test examples of the present invention, wherein Figure A is a statistical analysis diagram of licorice leaves; Figure B is a statistical analysis diagram of licorice plant height; Figure C is a statistical analysis diagram of licorice root depth; Figure D is a licorice biomass analysis diagram, "*" means P<0.05, "**" means P<0.01, and "***" means P<0.001. DETAILED DESCRIPTION

[0079] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0080] Example 1

[0081] This embodiment provides a microbial composition, which contains cyanobacteria (purchased from the freshwater algae species bank of the Chinese Academy of Sciences, Latin species name: Synechococcus7942, number FACHB-805) and actinomycetes (purchased from Beina Biotechnology, BNCC number BNCC368408), and the addition ratio of cyanobacteria to actinomycetes is 1:1; in the microbial composition, the concentration of microorganisms is 5×10 9 / mL, pH=7.0.

[0082] Example 2

[0083] This embodiment provides a screening method for the microbial composition in Embodiment 1, and the screening method is specifically as follows:

[0084] 1. Test materials

[0085] This study used sandy soil from the experimental area of ​​Liaohua Town, Xingzi County, Jiujiang City, Jiangxi Province (115°48′~116°10′E, 29°8′~29°36′N) as the material. The experimental area was divided into two areas, A and B. The growth years of Vitex simplex in area A were more than two years. In area B, the land was opened and leveled in March 2023, and the branches and stems of Vitex simplex re-rooted and sprouted, with a growth year of less than two years. The experimental area is located on the left bank of the flood channel into Poyang Lake. It belongs to the mid-subtropical humid area, with high temperature and heavy rainfall, obvious dry and wet seasons, and unreasonable seasonal precipitation distribution within the year. The annual average temperature is 16.5-17.8℃, the average surface temperature is 21.3℃, the highest surface temperature is 69.5℃, the annual evaporation is 1880mm, and the annual precipitation is 1300-1600mm. Precipitation is mainly in spring and summer, with less precipitation in autumn and winter. In winter, cold waves and northerly winds often blow, with wind speeds reaching 17m / s, causing the lake to shrink and the lake sand to be exposed. Wind erosion and water erosion provide the driving force for the formation of the sand dunes in Poyang Lake.

[0086] The soil of the desertified land of Poyang Lake is a unique subtropical desertified soil in southern China formed under the combined effects of historical geological and geomorphological changes, contemporary climate conditions, lake changes, and human activities. It mainly grows sandy vegetation that is resistant to barrenness and drought, mainly simple-leaved vitex, with a small amount of Cynodon dactylon (L.) Pers., Lespedeza thunbergii subsp. formosa (Vogel) H. Ohashi, and Artemisia capillaris Thunb., and has a low coverage.

[0087] 2. Screening method

[0088] (1) According to the growth characteristics of Vitex unifolia, data collection was conducted in two stages. The details are as follows:

[0089] The first sample collection time was in late September 2023. The collection area was the place where Vitex grows (exp.) and the place where Vitex does not grow (con.) in area B. The measurement depth, the distance from the ground and the sampling depth were all 10cm, 20cm and 30cm. The pH value and underground temperature were measured using a pH368 (resolution 0.01pH) soil acidity and alkalinity tester and an underground thermometer; the above-ground temperature and humidity were measured using an above-ground thermometer and hygrometer; soil samples from the test points were collected using a soil drill, and the soil at the same depth was evenly mixed for the determination of soil moisture content. At the same time, the survival rate and biomass of the planted licorice were counted by sampling. Licorice is a perennial herb, so the sample size is 5m×5m, and the number of licorice, Vitex and Sphaerocarpa in the sample was counted. A bacterial fertilizer experiment was conducted on licorice with good growth and no diseases and insect pests. 100 licorice plants with similar growth were selected and divided into two groups, one as the control group, which was watered with 500ml of water, and the other as the experimental group, which was watered with 500ml of bacterial water (see the experimental example for details of the plan).

[0090] The second sample collection time is mid-October 2023. In areas A (L) and B (X), samples of Vitex unifolia with good growth conditions and no pests and diseases are selected for sampling. The blank control (LCK, XCK) and peri-root soil (LGZ, XGZ) samples are sampled using the five-point sampling method, and soil samples 5-20 cm around the root system are collected. For rhizosphere soil (LGJ, XGJ) samples, a sterile soft-bristled paintbrush is used to gently brush away the soil tightly attached to the roots of Vitex unifolia.

[0091] Three independent soil samples were randomly selected from areas A and B and then pooled to form a composite sample. The composite soil sample was divided into two parts: one part was frozen in liquid nitrogen, transported to the laboratory with dry ice, and stored at -80 °C for bacterial and fungal determination; the remaining sample part was air-dried to determine the activity of soil enzymes.

[0092] The leaf and root endophyte samples were selected from leaves (LYN) and roots (LGN) with good growth in zone A (L). The sample surface was rinsed with running water to physically remove the surface bacteria, release and separate the endophytes; then, the samples were filtered and centrifuged, and the precipitates were collected and stored in liquid nitrogen to obtain pretreated samples for the determination of bacteria and fungi.

[0093] (2) Index determination

[0094] 1) Determination of soil enzyme activity

[0095] The total nitrogen (TN) content of the soil samples obtained in the above (1) was determined by the semi-micro Kjeldahl method; the alkaline diffusion method was used to determine the alkaline nitrogen (AN) content of the soil samples obtained in the above (1); the indophenol blue colorimetric method was used to determine the urease (UA) activity of the soil samples obtained in the above (1); and the microbial nitrogenase ELISA kit was used to determine the nitrogenase (NA) activity of the soil samples obtained in the above (1).

[0096] 2) DNA extraction, gene amplification, and high-throughput sequencing

[0097] The pretreated samples obtained in (1) above were used to extract nucleic acids using the OMEGA Soil DNA Kit (M5635-02) (Omega Bio-Tek, Norcross, GA, USA). The concentration and purification of the extracted DNA were detected using a NanoDrop 2000 UV-visible spectrophotometer (Thermo Scientific, USA), and the quality was tested using 1% agarose gel electrophoresis. For bacterial PCR amplification in soil samples, the 16S rRNA V3-V4 region was selected, and the primer sequences were 341F (5'-CCTACGGGNGGCWGCAG-3') and 805R (5'-GACTACHVGGGTATCTAATCC-3'). For bacterial PCR amplification in root and leaf samples, the primers specific to the 16S rRNA V5-V7 region were 799F (5'-AACMGGATTAGATACCCKG-3') and 1193R (5'-ACGTCATCCCCACCTTCC-3'). For fungal PCR amplification in soil samples, the ITS rDNA ITS region was selected, and the primer sequences were ITS1F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2R (5'-GCTGCGTTCTTCATCGATGC-3'). For fungal PCR amplification in root and leaf samples, the primers were ITS rDNA ITS1(b) region, primer sequences are ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3'), ITS2R (5'-GCTGCGTTCTTCATCGATGC-3'). ABI DNA fragment amplification was performed using a 9700 thermal cycler. The DNA was denatured at 98°C for 5 minutes, for a single cycle; then denatured at 98°C for 30 seconds, annealed at 52°C for 30 seconds, and extended at 72°C for 45 seconds, for a total of 28 cycles; finally, it was kept at 72°C for 5 minutes to allow the product to be fully extended and stored at 12°C.

[0098] The amplified products were screened by 2% agarose gel electrophoresis, and the target fragments were recovered by the magnetic bead recovery method. The gel-purified DNA was quantified on a Microplate reader (BioTek, FLx800) using the Quant-iT PicoGreen dsDNA Assay Kit, and then the samples were mixed according to the data required for each sample. The library was constructed using Illumina's TruSeq Nano DNA LT Library Prep Kit; the library was quality checked using the Agilent High Sensitivity DNA Kit; and the library was quantified using the Quant-iT PicoGreen dsDNA Assay Kit. For qualified libraries, 2×250bp double-end sequencing was performed using the NovaSeq 6000SP Reagent Kit (500 cycles).

[0099] (3) Data processing

[0100] The raw data of microbiome sequencing were spliced ​​and optimized using fastpv0.19.6 and FLASHv1.2.7 software, and the optimized data were further processed by the sequence denoising method DADA2 to obtain the amplicon sequence variants (ASV) in the samples. The ASV representative sequence information was classified and annotated using QIIME2 v2022.2 software with the bacterial 16S rRNA gene Silva database and the fungal ITS UNITE database as references.

[0101] Excel 2019 was used for data processing; R language v4.1.3 was used for data analysis; Origin2018 and GraphPad Prism 9 were used for graphing; SPSS25.0 software was used for one-way analysis of variance of the data, and the least-significant difference (LSD) method was used for the significance test, and the significance level was set at 0.05.

[0102] (4) Results analysis

[0103] 1) Analysis of the physical and chemical properties of Vitex simplex in the habitat of Area B

[0104] The results are as follows Figure 1 As shown in the figure, it can be seen that:

[0105] pH value at the same depth ( Figure 1As shown in Figure A, the pH value in the experimental group B area where Vitex grows (exp.) is always greater than that in the control group where Vitex does not grow (con.), and at depths of 20 cm and 30 cm, there are significant differences between the experimental and control groups; with the increase of depth, the pH value first decreases and then increases;

[0106] Soil moisture content at the same depth ( Figure 1 As shown in Figure B, the experimental group (exp.) is always smaller than the control group (con.), and there are significant differences between the experimental group and the control group at depths of 20 cm and 30 cm; with the increase of depth, the water content first increases and then decreases;

[0107] The ground temperature at the same height ( Figure 1 As shown in Figure C, the temperature of the experimental group (exp.) was always lower than that of the control group (con.), with a temperature difference of 4°C, and there was a significant difference between the experimental and control groups. With the increase of altitude, the ground temperature of both the experimental and control groups increased slightly, but there was no significant difference.

[0108] The ground humidity at the same height ( Figure 1 As shown in Figure D in the figure, the experimental group (exp.) was always greater than the control group (con.), and there were significant differences between the experimental and control groups at depths of 10 cm and 20 cm; with the increase in height, the ground humidity of both the experimental and control groups decreased, and the decrease was smaller;

[0109] The underground temperature at the same depth ( Figure 1 As shown in Figure E in the figure, the experimental group (exp.) was significantly lower than the control group (con.), and there were significant differences between the two groups. With the increase of depth, the underground temperature of both the experimental group and the control group decreased significantly, and there were significant differences.

[0110] In general, the pH value and aboveground humidity of the experimental group were greater than those of the control group, while the water content, aboveground temperature and underground temperature of the experimental group were lower than those of the control group. This shows that while absorbing and utilizing water, Vitex simplicifolia has a certain water-retaining effect on the sand dunes; it can also reduce the aboveground temperature, increase the aboveground humidity, and reduce the loss of water in the sand dunes, which has the effect of retaining water and cooling; it can also reduce the underground temperature, and the shallower the depth, the more significant it is, which is more conducive to the growth of itself or other plants.

[0111] 2) Physical and chemical properties of soil of Vitex unifolia in the same planting area

[0112] The results of soil physical and chemical properties analysis of Vitex unifolia in the same planting area are as follows Figure 2 As shown, from Figure 2 It can be seen that:

[0113] Total nitrogen ( Figure 2As shown in Figure A in the figure, the content of LGJ in the rhizosphere soil of area A was the highest, at 4.65 g / kg, followed by LGZ in the root periphery soil of area A. That is, in the habitat of single-leafed vitex, the total nitrogen content in area A was the highest, and both groups were significantly different from other groups (P<0.05);

[0114] Alkaline nitrogen ( Figure 2 The content of XCK in rhizosphere soil was significantly higher than that in peri-root soil, but there was no significant difference with the blank control XCK in area B.

[0115] Urease ( Figure 2 As shown in Figure C, the activity was the lowest in the blank control XCK in area B, and the urease activity in the rhizosphere soil LGJ in area A was higher than that in the other five treatments, with a urease activity of 462.70u / g, much higher than that in the other five treatments;

[0116] Nitrogenase ( Figure 2 (As shown in Figure D) There were significant differences among the components (P<0.05), and the content in the control group was higher.

[0117] In summary, except for nitrogenase, the contents of each substance showed rhizosphere soil>periroot soil>blank control, and the content in area A was greater than that in area B. This indicates that the growth of Vitex simplicifolia can increase the contents of total nitrogen, alkaline nitrogen and urease in sand hill soil, thereby improving the properties of sandy soil, increasing soil fertility and promoting plant growth; and its content continues to increase with the increase of planting years.

[0118] 3) Alpha diversity of soil microorganisms of Vitex simplicifolia in sand hill habitat

[0119] Alpha diversity reflects the species richness and diversity of the community, Chao1 index reflects the richness of the soil community, and Shannon index reflects the diversity of the community.

[0120] The results of the analysis are shown in Table 1. The Chao1 index and Shannon index of soil bacterial community showed that the rhizosphere soil> rhizosphere soil> blank control (LGZ>LGJ>LCK, XGZ>XGJ>XCK), and there were significant differences between the rhizosphere soil and the blank control (P<0.05); the Chao1 index and Shannon index of soil fungal community showed that the rhizosphere soil> rhizosphere soil (LGZ>LGJ, XGZ>XGJ>XCK); the soil microbial α diversity index showed that the rhizosphere soil> rhizosphere soil> blank control, and area B> area A. Among the endophytes of Vitex unifolia, the bacterial community LGN>LYN, and the fungal community LYN>LGN. The results showed that the richness and diversity of bacterial communities in the areas planted with Vitex unifolia (areas A and B) were higher than those in the blank control, and there were significant differences in most of them; with the increase of planting years, the bacterial richness and diversity decreased. The richness of fungal community in area A was lower than that in the blank control, while that in area B was higher than that in the blank control; there was no significant difference in the diversity of fungal community in the areas planted with single-leaved vitex (areas A and B).

[0121] Table 1 Alpha diversity of soil microorganisms in the habitat of Vitex simplex

[0122]

[0123] The values ​​in the table are mean ± standard deviation. Different lowercase letters after the values ​​in the same column indicate significant differences (P<0.05).

[0124] (4) Analysis of microbial community composition

[0125] By sequencing the microbiome of soil samples from areas A and B, a total of 24,172 bacterial optimized sequences and 6,456 fungal sequences were obtained, with average sequence lengths of 221 and 220 bp, respectively; by sequencing the microbiome of the roots and leaves of Vitex simplex, a total of 648 bacterial optimized sequences and 359 fungal sequences were obtained, with average sequence lengths of 228 and 219 bp, respectively.

[0126] 1) Analysis of soil bacterial community composition

[0127] After quality filtering and leveling of the sequencing data, a total of 33 bacterial phyla, 75 classes, 157 orders, 267 families, 416 genera, and 129 species were obtained.

[0128] The results of soil bacterial community composition analysis at the phylum level are as follows Figure 3 As shown, from Figure 3As can be seen from Figure A, at the phylum level, the dominant populations and proportions of soil bacteria in the rhizosphere soil are Actinobacteriota 24.01%, Proteobacteria 22.88%, and Acidobacteriota 18.39%; the dominant populations and proportions of rhizosphere soil are Actinobacteria 25.56%, Proteobacteria 21.56%, and Cyanobacteria 19.10%; the dominant populations and proportions of the control group are Actinobacteriota 12.45%, Acidobacteriota 18.59%, and Chloroflexi 32.31%. According to the literature, Actinobacteriota, Proteobacteria (22.88%), Cyanobacteria, and Firmicutes all have the function of nitrogen fixation.

[0129] The soil bacteria in each component were further analyzed. Figure 3 As can be seen from the BG diagram in Figure 1, Actinobacteriota ( Figure 3 B) Proteobacteria Figure 3 C) were significantly enriched in the rhizosphere and peri-root soil; Chloroflexi ( Figure 3 E), Firmicutes ( Figure 3 G) were significantly enriched in the control group, and most of them showed significant differences; Cyanobacteria ( Figure 3 F) was only significantly enriched in the rhizosphere soil, and there were significant differences.

[0130] Therefore, at the soil bacterial phylum level, the main nitrogen-fixing microorganisms screened out were Actinobacteriota, Proteobacteria and Cyanobacteria; in the poor sand hill soil, the growth of S. simplex provided a microenvironment for the growth and reproduction of microorganisms such as Actinobacteriota, Proteobacteria and Cyanobacteria, which is of great value to the ecological restoration of the sand hill.

[0131] The results of soil bacterial genus level detection are as follows Figure 4 As shown, from Figure 4It can be seen that soil bacteria at the genus level ( Figure 4 A) The dominant species and proportion of the meso-rhizosphere soil were Acidothermus, belonging to Actinobacteriota (6.21%); the dominant species and proportion of the rhizosphere soil were Chloroplast, belonging to Cyanobacteria (18.94%); the dominant species and proportion of the control group were Micrococcus (AD3), belonging to Chloroflexi (21.31%). Further analysis of soil bacteria in each component was performed ( Figure 4 BG), Micrococcus spp. Figure 4 B) was mainly enriched in the control group and had significant differences, Chloroplast ( Figure 4 C) were mainly enriched in the rhizosphere soil and had significant differences. The other four groups ( Figure 4 The rhizosphere soil of old soil (area A) accounted for the highest proportion in both DG and old soil, and was significantly different from that of new soil (area B) and the control group. Acidothermus and Mycobacterium both belonged to the phylum Actinobacteriota.

[0132] This suggests that, at the soil bacterial genus level, microorganisms such as Chloroplast, Acidothermus and Mycobacterium may have the function of biological nitrogen fixation.

[0133] 2) Analysis of soil fungal community composition

[0134] After quality filtering and leveling of the sequencing data, a total of 11 phyla, 24 classes, 54 orders, 95 families, 124 genera, and 106 species of fungi were obtained.

[0135] The results of soil fungal composition analysis at the phylum level are as follows: Figure 5 As shown by Figure 5 It can be seen that soil fungi at the phylum level ( Figure 5A) The dominant populations and proportions of meso-rhizosphere soil were Ascomycota (62.39%), Basidiomycota (16.37%), and Mucoromycota (7.13%); the dominant populations and proportions of rhizosphere soil were Ascomycota (65.56%), Basidiomycota (2.62%); the dominant populations and proportions of the control group were Ascomycota (49.30%), Basidiomycota (10.52%), and Chytridiomycota (3.56%). Among them, Ascomycota ( Figure 5 B) were the dominant phyla in each group and accounted for the largest proportion in the rhizosphere soil of the old soil; Basidiomycota ( Figure 5 C) accounted for the largest proportion in the rhizosphere soil of the new soil and was significantly different from other groups; Mucoromycota ( Figure 5 D) are mainly concentrated in the soil around the roots, Chytridiomycota ( Figure 5 E) were mainly concentrated in the Lao Tu control group, and both were significantly different from the other groups.

[0136] The results of soil fungi analysis at the genus level are as follows Figure 6 As shown, from Figure 6 It can be seen that soil fungi at the genus level ( Figure 6 A) The dominant populations and proportions of LGZ were Penicillium 7.83%, Gongronella 10.26%, and Agaricus 6.91%; the dominant populations and proportions of XGZ were Penicillium 13.76%, Gongronella 3.23%, and Aspergillus 7.10%; the dominant populations and proportions of LGJ were Penicillium 8.62%; the dominant populations and proportions of XGJ were Sphaerobolus 9.36%; the dominant populations and proportions of LCK were Calcarisporiella 2.94%; the dominant populations and proportions of XCK were Penicillium 6.03%.

[0137] The soil fungi in each component were further analyzed (results Figure 6 BE), Penicillium ( Figure 6B) were dominant phyla in XGZ, LGZ, and LGJ, and accounted for the largest proportion in XGJ; Aspergillus, Purpureocillium, and Gongronella ( Figure 6 CE) accounted for the largest proportion in the peri-root soil.

[0138] 3) Analysis of endophytic bacterial community composition in Vitex unifolia

[0139] After quality filtering and leveling of the roots and leaves of Vitex unifolia, 9 phyla, 16 classes, 37 orders, 43 families, 49 genera, and 4 species of bacteria were obtained, and 5 phyla, 9 classes, 16 orders, 21 families, 37 genera, and 19 species of fungi were obtained.

[0140] The results of the bacterial community detection of the root and leaves of Vitex unifolia at the phylum level are as follows Figure 7 As shown in the figure, it can be seen that at the door level ( Figure 7 A), the dominant phyla of root endophytes were Actinobacteriota (47.11%) and Proteobacteria (52.29%); the dominant phyla of leaf endophytes were Firmicutes (88.59%); at the genus level ( Figure 7 B), the dominant genus of root endophytes was Acidothermus (40.39%); the dominant genus of leaf endophytes was Lactobacillus (87.18%); the fungal community of the roots and leaves of Vitex unifolia ( Figure 7 CD), at the door level ( Figure 7 C), the dominant phyla of root endophytes were Ascomycota (62.82%) and Basidiomycota (35.20%); the dominant phylum of leaf endophytes was Ascomycota (78.28%); at the genus level ( Figure 7 D), there was no obvious dominant genera in the root endophytes; the dominant genera in the leaf endophytes were Pseudocercospora (16.33%) and Colletotrichum (7.87%).

[0141] 4) Microbial community principal coordinate analysis

[0142] The Bray Curtis distance algorithm was used to perform principal coordinate analysis of the microbial community, and similarity analysis was used to test the differences between groups. The principal coordinate analysis revealed the differences between and within groups of the microbial community. The analysis results are shown in the figure below. Figure 8 As shown in the figure, it can be seen that in the soil bacterial community ( Figure 8A) In the distribution of each group, the contribution of principal coordinates PC1 and PC2 were 27.29% and 14.74%, respectively. There were significant differences among the groups (P<0.001). LGJ, XGZ and XCK clustered together, and LGJ was far away from other groups, indicating that the bacterial community structure of the rhizosphere soil in area A changed greatly. The soil fungal community ( Figure 8 B), there were significant differences among the groups (P<0.001), and LGJ and XGJ clustered, while LCK and XCK clustered, indicating that the fungal community structure of the rhizosphere soil was similar, and the fungal community structure in each group was different from that in the blank control group; the bacterial and fungal communities of the roots and leaves of Vitex unifolia ( Figure 8 C and 8D) are both far away, that is, their bacterial and fungal community structures are different.

[0143] 5) Relationship between soil microbial communities and soil physical and chemical properties

[0144] The soil bacterial and fungal communities under different treatments were analyzed for redundancy based on the Bray-Curtis distance with the soil physicochemical properties.

[0145] The results are as follows Fig. 9 As shown, from Fig. 9 It can be seen that the soil physicochemical properties of total nitrogen (TN) content, alkaline nitrogen (AN) content, urease (UA) activity and nitrogenase (NA) activity are closely related to the distribution of soil bacterial and fungal communities. The first two ordination axes explained 68.72% (RDA1: 46.16%, RDA2: 22.56%) and 82.93% (RDA1: 53.43%, RDA2: 29.50%) of the variation, which can better reflect the relationship between soil physicochemical factors and soil bacteria and fungi levels. Among them, total nitrogen (TN) content and urease (UA) activity are closely related to soil bacterial community ( Fig. 9 The correlation between Actinobacteriota and Cyanobacteria and soil physicochemical properties was the largest, followed by alkaline nitrogen (AN) content, and the angles between Actinobacteriota and Cyanobacteria and soil physicochemical properties were AN>TN>AN>UA. The smaller the angle, the greater the correlation with soil microorganisms, that is, Actinobacteriota and Cyanobacteria had the largest correlation with urease (UA); soil fungal community ( Fig. 9 B), the correlation with alkaline nitrogen (AN) and nitrogenase (NA) activity is relatively large, and the angles between Ascomycota (Ascomycota) and Basidiomycota (Basidiomycota) and various soil physical and chemical properties are all obtuse, that is, the correlation with soil physical and chemical properties is relatively small.

[0146] 6) Different microbial species in the habitats of Vitex simplex in different regions

[0147] In order to explore the significantly different bacterial and fungal communities in the soils of different groups and the roots and leaves of single-leaf vitex, the LEfSe tool was used to analyze the microbial communities at different levels, and the species with a linear discriminant analysis (LDA) threshold greater than 4.0 were displayed in a bar graph.

[0148] The results are as follows Figure 10-12 As shown in the figure, it can be seen that in soil bacteria ( Fig.10 ), Burkholderiaceae (order to genus) was significantly enriched in XGJ; TK10 (class to genus) and AD3 (class to genus) were significantly enriched in LCK; IMCC26256 and Subgroup_2 were significantly enriched in XCK; WPS_2 (phylum to genus), Bryobacteraceae (order to genus), Pseudomonadaceae (order to genus), Mycobacteriaceae (order to genus) and WD2101_soil_group (order to genus) were significantly enriched in LGJ.

[0149] In soil fungi ( Fig.11 ), Venturiaceae (order to genus) and Cunninghamellaceae (class to genus) were significantly enriched in XGJ; Aspergillaceae (order to species) was significantly enriched in XGZ; Calcarisporiellaceae (order to genus) was significantly enriched in LCK.

[0150] Bacteria in the leaves of Vitex unifolia roots ( Fig.12 In C), Acidothermaceae (order to genus) was significantly enriched in LGN; Lactobacillaceae (order to genus) was significantly enriched in LYN.

[0151] Fungus on the leaves of Vitex unifolia ( Fig.12 D), Hypocreaceae (order to genus) was significantly enriched in LGN; Malasseziaceae (order to species) and Phyllostictaceae (order to species) were significantly enriched in LYN. The results showed that the bacterial and fungal communities in the soil and roots and leaves of Vitex unifolia in different groups were significantly different.

[0152] In summary, cyanobacteria and actinomycetes were screened and used as a combination for planting northern arid desert plants in southern humid deserts.

[0153] Example 3

[0154] This embodiment provides a method for planting northern arid desert plants in southern humid deserts, and the specific process is as follows:

[0155] (1) The lateral roots of the northern arid desert plant Glycyrrhiza glabra were excavated in mid-March. During transportation and storage, ventilation was maintained to prevent sun and rain. One day before planting, the lateral roots were cut into 10-15 cm (12 cm in this embodiment) lengths, Stanley carbendazim was used for dilution at 1:1000, soaked for 5 minutes, and then Guoguang rooting powder was diluted at 1:200 and soaked for 2 hours. Sandy soil (the same as in Example 2) was selected as the planting site, and the lateral roots were planted with a spacing of 20 cm between plants and 20 cm between rows. The planting depth was 20 cm, and the soil was covered with 20 cm of soil. The area was 1 m 2 .

[0156] (2) At 1m 2 Single-leaf Vitex spp. (20 cm in height, 75.2 plants / m2) were planted around the lateral roots of licorice. 2 ), the plant spacing is 10cm, and the distance between licorice and single-leaf vitex is 60cm.

[0157] (3) Field management after planting:

[0158] Field control of pests and diseases: Pest control should adhere to the principle of prevention first and integrated control first. Imported varieties must be strictly quarantined, with ecological control and biological control as the main methods, and chemical control should be used as little as possible. If chemical control is adopted, it must be strictly implemented in accordance with the provisions of GB4285 and GB / T8321, and high-risk pesticides and mixed pesticides banned by the state must not be used. Powdery mildew: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution or 50% methyl thiosalt 1500 times solution for prevention and control; Rust: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution; Brown spot disease: In the early stage of the disease, spray 75% thiocarb wettable powder 500-600 times solution or 50% carbendazim wettable powder 1000 times solution. Underground pests: When preparing the land, spray 1000-2000 times solution every 667m 2 Mix 500ml of thiocin with 3000ml of water, mix with 20kg of fine sand, spread evenly on the surface, and transfer to the soil for soil treatment; or dilute trichlorfon with 900g of water, mix into 10kg of oil residue and wheat bran mixture, spread on the surface, and kill underground pests during the peak period; for ground pests such as aphids, licorice leaf beetles, and short-haired grass weevils: spray pyrethroid insecticides 1000-1500 times. During the peak period, spray 2.5% cypermethrin solution 2000-4000 times.

[0159] Plant protection measures: During the high temperature and high humidity period (July-September), use fungicides (500-600 times of carbendazim, and this embodiment uses a 500-fold carbendazim solution) to kill fungi and molds.

[0160] Weeding and fertilization management: Pay attention to the first intercropping weeding when the seedling height is 5 cm. Field weeds should be weeded early and frequently in the first year, and no weeding is required after the second year; when all the seedlings have emerged and the seedling height is about 10-15 cm, apply phosphorus fertilizer or compound fertilizer 1-2 times before rainy days, each time 10kg / mu.

[0161] Example 4

[0162] This embodiment provides a method for planting northern arid desert plants in southern humid deserts, and the specific process is as follows:

[0163] (1) In mid-March, the lateral roots of the northern arid desert plant Glycyrrhiza uralensis were excavated. During transportation and storage, ventilation was maintained to prevent sun and rain. One day before planting, the lateral roots were cut into 10-15 cm (12 cm in this embodiment) lengths, Stanley carbendazim was used for dilution at 1:1000, soaked for 5 minutes, and then diluted with Guoguang rooting powder at 1:200 and soaked for 2 hours. Sandy soil was selected as the planting site, and the lateral roots were planted with a spacing of 20 cm between plants and 20 cm between rows. The planting depth was 20 cm, and the soil was covered with 20 cm of soil. The area was 1 m 2 .

[0164] (2) At 1m 2 Single-leaf Vitex spp. (20 cm in height, 58.0 plants / m2) were planted around the lateral roots of licorice. 2 ), the plant spacing is 10cm, and the distance between licorice and single-leaf vitex is 60cm.

[0165] (3) Field management after planting:

[0166] Field control of pests and diseases: Pest control should adhere to the principle of prevention first and integrated control first. Imported varieties must be strictly quarantined, with ecological control and biological control as the main methods, and chemical control should be used as little as possible. If chemical control is adopted, it must be strictly implemented in accordance with the provisions of GB4285 and GB / T8321, and high-risk pesticides and mixed pesticides banned by the state must not be used. Powdery mildew: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution or 50% methyl thiosalt 1500 times solution for prevention and control; Rust: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution; Brown spot disease: In the early stage of the disease, spray 75% thiocarb wettable powder 500-600 times solution or 50% carbendazim wettable powder 1000 times solution. Underground pests: When preparing the land, spray 1000-2000 times solution every 667m 2Mix 500ml of thiocin with 3000ml of water, mix with 20kg of fine sand, spread evenly on the surface, and transfer to the soil for soil treatment; or dilute trichlorfon with 900g of water, mix into 10kg of oil residue and wheat bran mixture, spread on the surface, and kill underground pests during the peak period; for ground pests such as aphids, licorice leaf beetles, and short-haired grass weevils: spray pyrethroid insecticides 1000-1500 times. During the peak period, spray 2.5% cypermethrin solution 2000-4000 times.

[0167] Plant protection measures: During the high temperature and high humidity period (July-September), use fungicides (500-600 times of carbendazim, and this embodiment uses a 500-fold carbendazim solution) to kill fungi and molds.

[0168] Weeding and fertilization management: Pay attention to the first intercropping weeding when the seedling height is 5 cm. Field weeds should be weeded early and frequently in the first year, and no weeding is required after the second year; when all the seedlings have emerged and the seedling height is about 10-15 cm, apply phosphorus fertilizer or compound fertilizer 1-2 times before rainy days, each time 10kg / mu.

[0169] Example 5

[0170] This embodiment provides a method for planting northern arid desert plants in southern humid deserts, and the specific process is as follows:

[0171] (1) In mid-March, the lateral roots of the northern arid desert plant Glycyrrhiza uralensis were excavated. During transportation and storage, ventilation was maintained to prevent sun and rain. One day before planting, the lateral roots were cut into 10-15 cm (12 cm in this embodiment) lengths, Stanley carbendazim was used for dilution at 1:1000, soaked for 5 minutes, and then diluted with Guoguang rooting powder at 1:200 and soaked for 2 hours. Sandy soil was selected as the planting site, and the lateral roots were planted with a spacing of 20 cm between plants and 20 cm between rows. The planting depth was 20 cm, and the soil was covered with 20 cm of soil. The area was 1 m 2 .

[0172] (2) At 1m 2 Single-leaf Vitex spp. (20 cm in height, 81.2 plants / m2) were planted around the lateral roots of licorice. 2 ), the plant spacing is 10cm, and the distance between licorice and single-leaf vitex is 60cm.

[0173] (3) Field management after planting:

[0174] Field control of pests and diseases: Pest control should adhere to the principle of prevention first and integrated control first. Imported varieties must be strictly quarantined, with ecological control and biological control as the main methods, and chemical control should be used as little as possible. If chemical control is adopted, it must be strictly implemented in accordance with the provisions of GB4285 and GB / T8321, and high-risk pesticides and mixed pesticides banned by the state must not be used. Powdery mildew: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution or 50% methyl thiosalt 1500 times solution for prevention and control; Rust: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution; Brown spot disease: In the early stage of the disease, spray 75% thiocarb wettable powder 500-600 times solution or 50% carbendazim wettable powder 1000 times solution. Underground pests: When preparing the land, spray 1000-2000 times solution every 667m 2 Mix 500ml of thiocin with 3000ml of water, mix with 20kg of fine sand, spread evenly on the surface, and transfer to the soil for soil treatment; or dilute trichlorfon with 900g of water, mix into 10kg of oil residue and wheat bran mixture, spread on the surface, and kill underground pests during the peak period; for ground pests such as aphids, licorice leaf beetles, and short-haired grass weevils: spray pyrethroid insecticides 1000-1500 times. During the peak period, spray 2.5% cypermethrin solution 2000-4000 times.

[0175] Plant protection measures: During the high temperature and high humidity period (July-September), use fungicides (500-600 times of carbendazim, and this embodiment uses a 500-fold carbendazim solution) to kill fungi and molds.

[0176] Weeding and fertilization management: Pay attention to the first intercropping weeding when the seedling height is 5 cm. Field weeds should be weeded early and frequently in the first year, and no weeding is required after the second year; when all the seedlings have emerged and the seedling height is about 10-15 cm, apply phosphorus fertilizer or compound fertilizer 1-2 times before rainy days, each time 10kg / mu.

[0177] Example 6

[0178] This embodiment provides a method for planting northern arid desert plants in southern humid deserts, and the specific process is as follows:

[0179] (1) The lateral roots of the northern arid desert plant Glycyrrhiza glabra were excavated in mid-March. During transportation and storage, ventilation was maintained to prevent sun and rain. One day before planting, the lateral roots were cut into 10-15 cm (12 cm in this embodiment) lengths, Stanley carbendazim was used to dilute it at 1:1000, soaked for 5 minutes, and then diluted with Guoguang rooting powder at 1:200 and soaked for 2 hours. Sandy soil (sand hills around Panyang Lake) was selected as the planting site, and lateral roots were planted with a spacing of 20 cm between plants and 20 cm between rows. The planting depth was 20 cm and the soil was covered with 20 cm of soil. The area was 1 m 2 .

[0180] (2) At 1m 2 Single-leaf Vitex spp. (20 cm in height, 82.2 plants / m2) were planted around the lateral roots of licorice. 2 ), the spacing between the single-leaf Vitex seedlings was 10 cm, and the planting distance between the single-leaf Vitex seedlings and the lateral roots of Licorice was 60 cm. From July to September, the microbial composition prepared in Example 1 was applied to Licorice once a month to increase resistance, and the application amount of the microbial composition was 500 mL.

[0181] (3) Field management after planting:

[0182] Field control of pests and diseases: Pest control should adhere to the principle of prevention first and integrated control first. Imported varieties must be strictly quarantined, with ecological control and biological control as the main methods, and chemical control should be used as little as possible. If chemical control is adopted, it must be strictly implemented in accordance with the provisions of GB4285 and GB / T8321, and high-risk pesticides and mixed pesticides banned by the state must not be used. Powdery mildew: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution or 50% methyl thiosalt 1500 times solution for prevention and control; Rust: In the early stage of the disease, spray 15% tripyrimidine 1000-1500 times solution; Brown spot disease: In the early stage of the disease, spray 75% thiocarb wettable powder 500-600 times solution or 50% carbendazim wettable powder 1000 times solution. Underground pests: When preparing the land, spray 1000-2000 times solution every 667m 2 Mix 500ml of thiocin with 3000ml of water, mix with 20kg of fine sand, spread evenly on the surface, and transfer to the soil for soil treatment; or dilute trichlorfon with 900g of water, mix into 10kg of oil residue and wheat bran mixture, spread on the surface, and kill underground pests during the peak period; for ground pests such as aphids, licorice leaf beetles, and short-haired grass weevils: spray pyrethroid insecticides 1000-1500 times. During the peak period, spray 2.5% cypermethrin solution 2000-4000 times.

[0183] Plant protection measures: During the high temperature and high humidity period (July-September), use fungicides (500-600 times of Stanley carbendazim can be used. This embodiment uses a 500-fold carbendazim solution, 100 g / mu, sprayed once every 30 days) to kill fungi and molds.

[0184] Weeding and fertilization management: Pay attention to the first intercropping weeding when the seedling height is 5 cm. Field weeds should be weeded early and frequently in the first year, and no weeding is required after the second year; when all the seedlings have emerged and the seedling height is about 10-15 cm, apply phosphorus fertilizer or compound fertilizer 1-2 times before rainy days, each time 10kg / mu.

[0185] Comparative Example 1

[0186] This comparative example provides a method for planting northern arid desert plants in southern humid deserts. The specific steps are different from those in Example 3 only in that the companion plant single-leaf Vitex serrata seedlings are replaced with bulbous column grass seedlings, wherein the number of the companion plant bulbous column grass is 45.00 plants / m 2 .

[0187] Comparative Example 2

[0188] This comparative example provides a method for planting northern arid desert plants in southern humid deserts. The specific steps are different from those in Example 3 only in that the companion plant single-leaf Vitex serrata seedlings are replaced with bulbous column grass seedlings, wherein the number of the companion plant bulbous column grass is 31.20 plants / m 2 .

[0189] Comparative Example 3

[0190] This comparative example provides a method for planting northern arid desert plants in southern humid deserts. The specific steps are different from those in Example 3 only in that the semi-living plant single-leaf Vitex serrata seedlings are replaced with bulbous column grass seedlings, wherein the number of the companion plant bulbous column grass is 31.20 plants / m 2 .

[0191] Comparative Example 4

[0192] This comparative example provides a method for planting northern arid desert plants in southern humid deserts, and the specific steps are different from those of Example 3 only in that there is no companion plant, Vitex unifolia seedlings.

[0193] Test example

[0194] 1. Effect of intercropping of licorice and Vitex unifolia on the survival rate and biomass of licorice in southern humid deserts

[0195] This test example tests the methods of Examples 3-5 and Comparative Examples 1-4, and statistically analyzes the survival rate and biomass of Glycyrrhiza glabra planted in the southern humid desert (Poyang Lake Sand Hill) for 4 months. The field growth verification pattern of three growth patterns of Glycyrrhiza glabra and Bulbostylis barbata, Glycyrrhiza glabra and Vitexrotundifolia Lf, and Glycyrrhiza glabra alone is shown in the figure. Fig.13 Comparative Examples 2 and 3 are actually repeated tests of the companion plant Cyperus sphaerocarpa.

[0196] Table 2 Effects of different companion plants on the survival rate and biomass of licorice

[0197]

[0198] The results of the effects of different methods on the survival rate and biomass of licorice planted in the southern humid desert are shown in Table 2. It can be seen from the table that during the growth of licorice, the survival rate and height of licorice in the ball-column grass sample plot were the lowest, and the grass competed for the water and nutrients of licorice; the survival rate and height (biomass) of licorice in the single-leaf vitex sample plot were the highest, which may play a role in water conservation and water conservation. The proportion of single-leaf vitex has different effects on the growth promotion of licorice. Therefore, clarifying the intercropping ratio of licorice and vitex rotundifolia is important for the growth of licorice.

[0199] 2. Effect of the position of Licorice and Vitex unifolia on the plant height of Licorice

[0200] Grouping: surrounding planting group, planting above group, planting below group, planting on the left group, planting on the right group, blank group;

[0201] Surrounding planting group: 4 single-leaved vitex were planted around a licorice plant (above, below, left and right), with a planting distance of 60 cm;

[0202] In the upper planting group: a single-leaf vitex was planted above a licorice plant, with a planting distance of 60 cm;

[0203] In the bottom planting group: a single-leaf vitex was planted under a licorice plant, with a planting distance of 60 cm;

[0204] In the left planting group: a single-leaf vitex was planted on the left side of a licorice plant, with a planting distance of 60 cm;

[0205] In the right planting group: a single-leaf vitex is planted on the right side of a licorice plant, with a planting distance of 60 cm;

[0206] Blank group: Vitex simplex was not planted around licorice.

[0207] Experimental steps: 15 mu of Glycyrrhiza glabra was randomly planted in a simple-leaved vitex base in the southern humid desert (Poyang Lake sand hill), and treated separately according to the above grouping conditions. For specific experimental steps, see Example 3, 30 replicates per group, and biomass statistics were performed after 4 months (N>=6).

[0208] The plant height of licorice was measured in the environment of single-leaf vitex plants at different locations. The results are as follows: Fig.14 As shown in the figure, it can be seen that the licorice planted with Vitex single-leaf around it has a height of 59.90cm, the highest height and the best growth state. The occurrence of this phenomenon is related to the water retention of Vitex single-leaf. The licorice grown together with Vitex single-leaf has the shortest height because of the competition for water and nutrients. In other sample plots, the licorice with Vitex single-leaf located on the top has the highest height and has a certain water-locking function.

[0209] 3. Effect of microbial composition on licorice biomass accumulation

[0210] This test example tests the method of Example 6 to plant licorice in the southern humid desert (sand hills around Poyang Lake). The difference from the steps of the example is that the step "(1) digging the lateral roots of the northern plant Glycyrrhiza glabra in mid-March, and keeping ventilation during transportation and storage to prevent sun and rain. One day before planting, cut the lateral roots into 10-15 cm (12 cm in this example) lengths, soak them in a working concentration of carbendazim solution for 5 minutes, and then soak them in a rooting powder solution for 2 hours." is omitted. Instead, Glycyrrhiza glabra seedlings with poor growth conditions and a growth height of 5 cm are selected for the experiment. The survival rate and biomass after 4 months of planting are calculated. The difference between the control group and the above method is that an equal amount of clean water is applied. The experimental figure is as shown in the figure below. Fig.15 shown.

[0211] The results are as follows Fig.16 As shown in the figure, it can be seen that the survival rate, height and number of leaves of licorice applied with the microbial composition prepared in Example 1 are higher than those of the watered control group, and there are significant differences. Therefore, the application of bacterial fertilizer has a certain promoting effect on the growth and development of licorice.

[0212] 4. Growth cycle of medicinal licorice

[0213] This test example tests the method of Example 6, planting licorice in the southern humid desert (sand hills around Poyang Lake), and monitoring the growth cycle of medicinal licorice (Glycyrrhiza spp.) from germination to maturity throughout the season. Figure 17-18 shown.

[0214] from Fig.17 As can be seen in the figure, liquorice root planted in sandy soil begins to grow noticeably within two weeks, with the first true leaves appearing around the third week. By the second month, the plant has obvious lateral root development, and by the end of the vegetative period the plant reaches an average height of 20-25 cm. There are no obvious pest or fungal infections at this stage. Flowering is usually uniform, with no major changes in plant height. The plant devotes most of its energy to root development, which shows a high capacity for nutrient absorption. The presence of nodules indicates nitrogen fixation activity. Typically, after 6 months, the above-ground parts of the plant mostly dry up, marking the end of the active growth period. Throughout the growth cycle, environmental factors such as temperature, soil pH, and moisture levels influence optimal growing conditions.

[0215] from Fig.18 It can be seen that, taking Glycyrrhiza glabra as an example, at different stages of the year (April, July and October), the leaves in the second year increased by 2-3 times compared with the first year, the plant height increased insignificantly, the root depth also increased significantly, and the total biomass (plant dry weight) increased to about 2 times.

[0216] Poyang Lake is an important wetland in the world, and its development is of great significance to the ecological construction of Jiangxi and even the whole country. In the late Pleistocene, the active winter monsoon accumulated sand dunes, and received water and sand from the five major rivers in Jiangxi Province. In addition, it has been damaged by human activities for a long time, which together formed a "sand mountain" of about 38,900 hectares around Poyang Lake. The sand mountain around Poyang Lake not only threatens the production and life of local residents, but also affects the flood discharge and smooth waterway of Poyang Lake, seriously hindering the sustainable development of the local economy and ecology.

[0217] Licorice is a legume plant, mainly including the dried roots and stems of Glycyrrhiza uralensis, Glycyrrhiza inflata and Glycyrrhiza glabra L., which has the effects of invigorating qi and tonifying the middle, removing phlegm and relieving cough, relieving acute pain, clearing away heat and detoxifying, and harmonizing medicinal properties. Licorice is an important raw material for the pharmaceutical and food industry, and is an important strategic resource in the food coloring, flavoring, sweetener, cosmetics and other industries. At present, the market is often unable to supply products due to shortage of raw materials.

[0218] How to introduce various types of licorice into the Poyang Lake sand dunes to achieve both ecological and economic benefits has become a key issue. The present invention creatively proposes a method for planting licorice in the southern humid desert, providing important theoretical support for the utilization of sand dunes and ecological restoration and reconstruction.

[0219] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A method for planting northern arid desert plants in southern humid deserts, characterized in that: The method comprises the following steps: planting northern arid desert plants and Vitex simplex together in southern humid desert.

2. The method according to claim 1, characterized in that The planting distance between the northern arid desert plant and the simple-leaved vitex is 50-70 cm.

3. The method according to claim 1, characterized in that The method further comprises the step of applying the microbial composition to the boreal arid desert plants; Preferably, the microbial composition comprises cyanobacteria and actinomycetes, and the addition ratio of the cyanobacteria to the actinomycetes is (1-2): (1-3); Preferably, the concentration of microorganisms in the microbial composition is 3×10 9 / mL-8×10 9 / mL. Preferably, the application amount of the microbial composition is 300-800 mL / plant.

4. The method according to claim 1, characterized in that: The planting of the northern arid desert plant adopts lateral roots; the length of the lateral roots is 10-15 cm.

5. The method according to claim 1, characterized in that The height of the single-leaf vitex is 5-25 cm; And / or, the planting density of the single-leaf vitex is 50-85 plants / m 2 .

6. The method according to claim 1, characterized in that The northern arid desert plants include liquorice; And / or, the southern humid desert includes the sand dunes around Poyang Lake.

7. Use of the method according to any one of claims 1 to 6 in restoring southern humid deserts.

8. A microbial composition, characterized in that The microbial composition comprises cyanobacteria and actinomycetes, and the addition ratio of the cyanobacteria to the actinomycetes is (1-2): (1-3); Preferably, the microbial composition comprises cyanobacteria and actinomycetes, and the addition ratio of the cyanobacteria to the actinomycetes is (1-2): (1-3); Preferably, the concentration of microorganisms in the microbial composition is 3×10 9 / mL-8×10 9 / mL.

9. A method for preparing the microbial composition according to claim 8, characterized in that: The preparation method comprises the following steps: mixing cyanobacteria and actinomycetes to obtain the product.

10. Use of the microbial composition according to any one of claims 8 in any one of the following: (1) Improve the survival rate of plants; (2) Promote plant growth; (3) Increase the number of plant leaves; (4) Restoration of the southern humid desert; (5) Prepare reagents for repairing southern humid deserts.

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