A solenopsis invicta bait and a preparation method and application thereof

Abstract

The present application belongs to the field of prevention and control of Solenopsis invicta Buren, and particularly relates to a bait for preventing and controlling Solenopsis invicta Buren, a preparation method and application thereof. The composition is indoxacarb and spinosad, and the mass ratio of spinosad to indoxacarb is 5:1-1:5. The bait adopts a puffing process, has light texture, and does not affect the attraction effect on Solenopsis invicta Buren in an unfavorable environment. The present application also provides a preparation method of the bait.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of prevention and control of Solenopsis invicta Buren, and particularly relates to a bait for preventing and controlling Solenopsis invicta Buren and a preparation method and application thereof. BACKGROUND

[0002] Solenopsis invicta Buren is an invasive biological species of Formicidae in Hymenoptera. Due to the lack of natural enemies, Solenopsis invicta Buren has a high invasion speed and great harm. In order to effectively prevent and control the reproduction and spread of Solenopsis invicta Buren, it is currently a research focus to develop a highly efficient bait for Solenopsis invicta Buren.

[0003] Existing prevention and control measures mainly include chemical bait control. The bait for killing Solenopsis invicta Buren has the advantages of simple operation, safety and target determination, and can achieve the effect of preventing and controlling Solenopsis invicta Buren. However, the existing bait has poor attractability to Solenopsis invicta Buren, and Solenopsis invicta Buren often refuses to eat. The attractability of the bait for Solenopsis invicta Buren is a problem to be solved.

[0004] The active ingredient for preventing and controlling Solenopsis invicta Buren is single indoxacarb, which promotes the occurrence of the drug resistance of Solenopsis invicta Buren. It is also an urgent problem to be considered to enrich the pesticide for preventing and controlling Solenopsis invicta Buren and slow down the occurrence of the drug resistance of Solenopsis invicta Buren. Therefore, it is of great significance to develop a compounded bait. SUMMARY

[0005] The present application solves the technical problems in the prior art and provides a bait for preventing and controlling Solenopsis invicta Buren and a preparation method and application thereof.

[0006] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0007] A composition for preventing and controlling Solenopsis invicta Buren, wherein the composition is indoxacarb and spinosad, and the mass ratio of indoxacarb to spinosad is 5:1-1:5.

[0008] The application of the composition for preventing and controlling Solenopsis invicta Buren in the preparation of a bait for Solenopsis invicta Buren.

[0009] A bait for Solenopsis invicta Buren, wherein the bait for Solenopsis invicta Buren is bait, an active ingredient and an attractant ingredient, and the attractant ingredient is farnesene and / or 2-ethyl-3,6-dimethylpyrazine.

[0010] The bait for Solenopsis invicta Buren contains 0.05%-0.2% of the active ingredient, 0.01%-0.02% of the attractant ingredient, and the rest is bait.

[0011] Preferably, the mass ratio of the active ingredient spinosad to indoxacarb is 1:2.5.

[0012] The mass ratio of farnesene to 2-ethyl-3,6-dimethylpyrazine in the attractant ingredient is 2:1-1:2, and preferably 1:1.5.

[0013] The farnesene is (Z, E)-alpha-farnesene.

[0014] The bait is one or more of corn meal, peanut meal, meat meal, and yeast glucan,

[0015] The bait for Solenopsis invicta Buren further comprises a preservative, wherein the preservative is added in an amount of 0.3% to 0.5% of the mass of the bait for Solenopsis invicta Buren.

[0016] The preservative is benzoic acid and / or citric acid.

[0017] The application of the bait for Solenopsis invicta Buren in the field of prevention and control of Solenopsis invicta Buren.

[0018] The bait for Solenopsis invicta Buren is prepared as an expanded granule.

[0019] The raw materials are mixed uniformly according to the percentages, and then are ultra-micronized, and then are added with water and extruded by an extruder to obtain the expanded granular bait for Solenopsis invicta Buren.

[0020] The bait is applied by scattering, and 20 to 25 g is scattered in each nest when the bait is used to control Solenopsis invicta Buren.

[0021] The bait has good attractivity to Solenopsis invicta Buren, and improves the control effect; the two insecticides with different action mechanisms are compounded, and the specific auxiliary material is added, which can also delay the resistance, and provides a new method for prevention and control of Solenopsis invicta Buren, delay of resistance and enhancement of attractivity; meanwhile, the bait is prepared by the expansion process, has light texture, and does not affect the attractivity to Solenopsis invicta Buren in the adverse environment, improves the rain washing resistance of the bait, and solves the problems of difficulty in carrying and large influence of the environment on the conventional bait DETAILED DESCRIPTION

[0022] The specific implementation of the technical scheme of the present application is described in detail below, but the present application is not limited to the following description, and the present application is described below in combination with specific examples.

[0023] The expanded granular bait is prepared by mixing the raw materials uniformly according to the percentages, and then ultra-micronizing, and then adding water and extruding by an extruder to obtain the expanded granular bait.

[0024] The ultra-micronization is performed by an air flow pulverizer at a speed of 50 g to 100 g per minute, and an air pressure of 0.4 mPa to 0.6 mPa.

[0025] The extrusion condition of the extruder is that the temperature is about 100 DEG C, and the extrusion is performed by a double-screw extruder.

[0026] Preparation of blank Solenopsis invicta bait:

[0027] Example 1

[0028] The blank Solenopsis invicta bait was corn grits 1000 g.

[0029] Example 2:

[0030] The blank Solenopsis invicta bait was peanut pieces 100 g, corn grits to make 1000 g.

[0031] Example 3:

[0032] The blank Solenopsis invicta bait was peanut pieces 100 g, meat meal 100 g, corn grits to make 1000 g.

[0033] Example 4:

[0034] The blank Solenopsis invicta bait was peanut pieces 100 g, meat meal 100 g, yeast glucan 100 g, corn grits to make 1000 g.

[0035] Example 5:

[0036] The blank Solenopsis invicta bait was (Z,E)-a-farnesene 0.08 g, 2-ethyl-3,6- dimethylpyrazine 0.12 g, peanut pieces 100 g, meat meal 100 g, yeast glucan 100 g, corn grits to make 1000 g.

[0037] Example 6:

[0038] The blank Solenopsis invicta bait was (Z,E)-a-farnesene 0.04 g, 2-ethyl-3,6- dimethylpyrazine 0.06 g, peanut pieces 100 g, meat meal 100 g, yeast glucan 100 g, corn grits to make 1000 g.

[0039] Example 7:

[0040] The blank Solenopsis invicta bait was (Z,E)-a-farnesene 0.12 g, 2-ethyl-3,6- dimethylpyrazine 0.08 g, peanut pieces 100 g, meat meal 100 g, yeast glucan 100 g, corn grits to make 1000 g.

[0041] Example 8:

[0042] The blank Solenopsis invicta bait was (Z,E)-a-farnesene 0.06 g, 2-ethyl-3,6- dimethylpyrazine 0.04 g, peanut pieces 100 g, meat meal 100 g, yeast glucan 100 g, corn grits to make 1000 g.

[0043] Comparative Example 1:

[0044] Blank Solenopsis invicta bait is (E,E)-α-farnesene 0.06 g, 2-ethyl-3,6-dimethylpyrazine 0.04 g, peanut pieces 100 g, meat powder 100 g, yeast glucan 100 g, and corn meal to 1000 g.

[0045] Verification test: attraction effect test

[0046] (1) Test object: Solenopsis invicta

[0047] (2) Test method: same as field test method.

[0048] (3) Test agent:

[0049] Examples 1 to 8 and Comparative Example 1.

[0050] (4) Data statistics:

[0051] Table 1: Results of attraction test of several treatments on Solenopsis invicta

[0052] Treatment group 24h Solenopsis invicta transport rate Example 1 85％ Example 2 86％ Example 3 92％ Example 4 90％ Example 5 95％ Example 6 96％ Example 7 94％ Example 8 95％ Comparative Example 1 75％

[0053] The test results show that the ordinary food bait can attract Solenopsis invicta, and the transportation rate of Solenopsis invicta is more than 80%; the transportation rate of the food bait with the added attractant is more than 90%. The results show that the attractant component can enhance the attraction of the bait to Solenopsis invicta. However, only in a specific attractant component can the bait achieve the effect, and the data of Comparative Example 1 show that (E,E)-α-farnesene has no attraction effect, and only (Z,E)-α-farnesene configuration has an attraction effect.

[0054] Preparation of Solenopsis invicta bait:

[0055] Example 1: 0.025% spinosad·0.025% indoxacarb bait

[0056] The Solenopsis invicta bait is spinosad 0.25 g, indoxacarb 0.25 g, (Z,E)-α-farnesene 0.08 g, 2-ethyl-3,6-dimethylpyrazine 0.12 g, benzoic acid 30 g, peanut pieces 100 g, and corn meal to 1000 g.

[0057] Example 2: 0.02% spinosad·0.05% indoxacarb bait

[0058] The Solenopsis invicta bait is spinosad 0.2 g, indoxacarb 0.5 g, (Z,E)-α-farnesene 0.04 g, 2-ethyl-3,6-dimethylpyrazine 0.06 g, benzoic acid 40 g, peanut pieces 100 g, meat powder 100 g, yeast glucan 100 g, and corn meal to 1000 g.

[0059] Example 3: 0.05% spinosad·0.02% indoxacarb bait

[0060] The Solenopsis invicta bait is spinosad 0.5 g, indoxacarb 0.2 g, (Z, E)-a-farnesene 0.075 g, 2-ethyl-3, 6-dimethylpyrazine 0.075 g, citric acid 30 g, peanut pieces 100 g, and corn grits to make up 1000 g.

[0061] Example 4: 0.05% spinosad · 0.05% indoxacarb bait

[0062] The Solenopsis invicta bait is spinosad 0.5 g, indoxacarb 0.5 g, (Z, E)-a-farnesene 0.075 g, 2-ethyl-3, 6-dimethylpyrazine 0.075 g, benzoic acid 50 g, peanut pieces 100 g, meat powder 100 g, and corn grits to make up 1000 g.

[0063] Example 5: 0.1% spinosad · 0.1% indoxacarb bait

[0064] The Solenopsis invicta bait is spinosad 1 g, indoxacarb 1 g, (Z, E)-a-farnesene 0.1 g, 2-ethyl-3, 6-dimethylpyrazine 0.1 g, benzoic acid 50 g, peanut pieces 100 g, meat powder 100 g, yeast glucan 100 g, and corn grits to make up 1000 g.

[0065] Comparative Example 1: 0.05% indoxacarb bait

[0066] The commercially available Jiangsu Gongcheng product is Xing'an.

[0067] The technical effects of the present application are described below in connection with specific tests:

[0068] Verification Test 2: Co-toxicity coefficient test

[0069] (1) Test object: Solenopsis invicta

[0070] (2) Test method: Refer to NY / T 1154.7-2006 "Pesticide indoor biological test guidelines insecticides Part 7: mixed joint action determination"

[0071] (3) Data statistics: The test agent was first dissolved with dichloromethane, then serially diluted with 0.1% Triton-X100 aqueous solution at a ratio of 1:9, 1 mL of each diluted concentration of the drug solution was added to a 150 mL serum bottle, the serum bottle was constantly rotated to evenly distribute the drug solution on the inner wall of the bottle, and was placed in a fume hood overnight to completely volatilize the organic solvent. The concentration interval of 100% death and 100% survival of the red imported fire ant was determined. Within this interval, appropriate concentrations were serially diluted with 0.1% Triton-X100 aqueous solution. 1 mL of each diluted concentration of the drug solution was added to a 150 mL serum bottle, the serum bottle was constantly rotated to evenly distribute the drug solution on the inner wall of the bottle, and was placed in a fume hood overnight to completely volatilize the organic solvent. Then vaseline was smeared on the neck of the serum bottle, 20 test insects were put into each bottle, and the experiment was repeated 5 times. The corresponding solvent treatment was used as the control group. After 1 h, all the test insects were transferred to clean vessels for standard feeding, and the number of deaths was checked and counted after 24 h. Statistical analysis software SPSS was used for analysis, and the LC 50 and 95% confidence limit values of each agent were calculated. The determination results are shown in the following table:

[0072] Table 2 Co-toxicity coefficients of spinosad and indoxacarb against red imported fire ants From Table 2, it can be seen that: when the complexing ratio of spinosad:indoxacarb is 5:1 to 1:5, the co-toxicity coefficient against red imported fire ants is greater than 120, showing a synergistic effect; when the complexing ratio of spinosad:indoxacarb is 1:2.5, the synergistic effect is obvious.

[0073] Verification test 2: field efficacy test

[0074] (1) Test object: red imported fire ants

[0075] (2) Test method: equal-sized red imported fire ant nests were selected, and the sample was applied at 20 g per nest.

[0076] (3) Test agent:

[0077] Control example 1: 0.05% indoxacarb bait (commercially available); examples 1-5.

[0078] (4) Data statistics:

[0079] Table 3 Efficacy test results of several examples on red imported fire ants

[0080] Treatment group Overall control effect Example 1 90％ Example 2 95％ Example 3 95％ Example 4 90％ Example 5 90％ Comparative Example 1 70％

[0081] The test results show that the preferred complexing ratio of the complexing agent has a prevention effect of 90% or more on red imported fire ants, and the effect is obvious.

[0082] The above examples are only for illustrating the technical concept and technical features of the present application, and cannot limit the protection scope of the present application. Any equivalent transformation or modification according to the essence of the present application should be covered in the protection scope of the present application.

Claims

1. A Solenopsis invicta bait, characterized in that, The red ant bait is a bait, a red ant prevention and control composition and an attracting component, wherein the red ant prevention and control composition is indoxacarb and spinosad, and the mass ratio of the spinosad to the indoxacarb is 5:1-1:5; The attractant component is (Z,E)-alpha - farnesene and 2-ethyl-3,6-dimethylpyrazine; (Z,E)-alpha - farnesene to 2-ethyl-3,6-dimethylpyrazine in a mass ratio of 2:1 to 1 :

2.

2. The Solenopsis invicta bait of claim 1, wherein, The red ant bait contains 0.05-0.2% of active ingredients and 0.01-0.02% of attracting components by weight, and the rest is bait.

3. The Solenopsis invicta bait of claim 1 or 2, wherein, The red ant bait further contains a preservative, wherein the addition amount of the preservative accounts for 0.3-0.5% of the mass of the red ant bait.

4. The Solenopsis invicta bait of claim 3, wherein, The preservative is benzoic acid and / or citric acid.

5. The use of the Solenopsis invicta bait of claim 1, wherein, The red ant bait is applied in the field of red ant prevention and control.

6. The use of a Solenopsis invicta bait according to claim 5, wherein the bait is a bait according to any one of claims 1 to 4. The red ant bait is prepared into puffed particles.

7. The use of a Solenopsis invicta bait according to claim 6, wherein the bait is a bait according to any one of claims 1 to 5. The raw materials are fully mixed according to the above percentages, and then are ultra-finely pulverized; water is added to the pulverized materials, and then the materials are puffed and granulated by a puffing machine to obtain the puffed particle red ant bait.