13C continuous stable isotope labeling device and method capable of controlling temperature and humidity

Abstract

The invention relates to the technical field of stable isotope tracing, and particularly discloses a temperature and humidity control 13C continuous stable isotope labeling device and method.The temperature and humidity control 13C continuous stable isotope labeling device comprises a labeling chamber, a concentration control assembly is arranged on one side of the labeling chamber, and a concentration monitoring assembly is arranged on one side of the concentration control assembly; a humidification assembly is arranged on the side, away from the concentration control assembly, of the concentration monitoring assembly, a dehumidification assembly is arranged on the side, away from the concentration monitoring assembly, of the humidification assembly, and an air pressure balance assembly is arranged on the side, away from the humidification assembly, of the dehumidification assembly. According to the device, accurate comprehensive regulation and control of multiple environmental factors such as gas concentration, temperature, humidity and air pressure in the device are realized, an ideal and accurate experimental environment is created for researching interaction of a plant-soil system under different environmental conditions, and the problems of poor temperature and humidity regulation effect and the like in the prior art are effectively solved.

Description

Technical Field

[0001] The present invention relates to the field of stable isotope tracing technology, and in particular to a temperature and humidity control 13 C continuous stable isotope labeling device and method. Background Art

[0002] Against the backdrop of changes in climate factors such as rising carbon dioxide concentrations, warming temperatures, and uneven precipitation, the response patterns and mechanisms of plant-soil interactions to such changes have become a research focus. Plant-soil interactions are mainly mediated by plant root secretions, which originate from atmospheric carbon dioxide fixed by photosynthesis and are used and transformed by microorganisms after being transferred to the underground part, thereby driving soil material turnover to ensure the nutritional needs of plant growth. Carbon isotope tracing is a common and effective technical means to explore plant-soil interaction patterns and mechanisms. Compared with traditional radioactive carbon isotope tracing technology, 13 C stable isotopes have the advantages of being pollution-free and highly safe. They are a commonly used tool for studying carbon migration, turnover and redistribution in ecosystems. They are of great significance in revealing the distribution and fate of plant photosynthetic products in plant-soil systems under climate change.

[0003] The patent document with the publication number "CN118897047A" discloses a device for stable isotope labeling, including a labeling chamber; a sensor assembly, a gas regulating assembly, a temperature regulating assembly and a humidity regulating assembly are arranged on the inner wall of the labeling chamber; a gas introduction assembly is installed on the outer wall of the labeling chamber; a light source assembly and a monitoring assembly are installed on the top wall of the labeling chamber; a reaction box and a plurality of cultivation pots are arranged on the bottom plate of the labeling chamber, and the target plants are planted in the plurality of cultivation pots; a plurality of universal wheels are arranged at the lower end of the bottom plate of the labeling chamber. The above invention installs temperature, air pressure and humidity sensors in the labeling chamber, and cooperates with the display on the outside to observe the parameters inside the labeling chamber in real time; and installs humidity, temperature and gas ducts in the labeling chamber to adjust the temperature, humidity and labeling gas concentration inside the labeling chamber in real time, so as to better study the distribution of carbon elements in plants and soil.

[0004] However, there are still defects in the existing technology: first, it is difficult to accurately control the atmospheric temperature and humidity in the device, and it is impossible to effectively simulate the climatic conditions under the changes of relevant climate factors; second, it is impossible to independently control the soil moisture of the culture pots in the device, and water irrigation is mostly based on experience, which affects the repeatability and accuracy of the test; third, the water tank seal will reduce the utilization efficiency of isotope carbon dioxide due to changes in air pressure in the device and the exchange of gas in the sealed water tank, resulting in waste of reagents.

[0005] Therefore, the existing 13 The main problems of the C continuous stable isotope labeling device are poor temperature and humidity adjustment effect, unsuitability for repeated experiments, and waste of experimental materials. Summary of the invention

[0006] The present invention is to solve the existing 13 In order to solve the problems existing in the 13C continuous stable isotope labeling device, the present invention proposes a 13C continuous stable isotope labeling device and method for controlling temperature and humidity, so as to achieve precise control of temperature and humidity and facilitate long-term experiments on plants.

[0007] The first technical solution of the present invention: temperature and humidity control 13 C continuous stable isotope labeling device, comprising a labeling chamber, a concentration control component is arranged on one side of the labeling chamber, a concentration monitoring component is arranged on one side of the concentration control component, a humidifying component is arranged on the side of the concentration monitoring component away from the concentration control component, a dehumidifying component is arranged on the side of the humidifying component away from the concentration monitoring component, and a gas pressure balance component is arranged on the side of the dehumidifying component away from the humidifying component;

[0008] The marking chamber comprises an upper box body, and a lower box body is arranged below the upper box body;

[0009] A diaphragm pressure gauge is arranged at the top edge of the upper box body, a heating component is arranged at the top center of the upper box body, and a heat dissipation component is arranged on the side wall of the lower box body; the diaphragm pressure gauge in the present invention can select a YE-100 pressure vacuum gauge;

[0010] A regulating component is arranged on the inner side of the lower box body, a culture pot is arranged above the regulating component, and a digital display soil temperature and humidity meter is arranged on the side of the culture pot; the digital display soil temperature and humidity meter in the present invention is a detection device integrating multiple functions, which can detect multiple data such as soil temperature and humidity, pH value, ambient temperature and humidity, etc.; further, a KS-SHTE temperature and humidity sensor can be selected for temperature and humidity detection;

[0011] A tray is provided below the culture pot.

[0012] Through the synergistic effect of various components, the present invention can comprehensively regulate the gas concentration, temperature, humidity, air pressure, etc. in the device, and can monitor the temperature and humidity inside the device in real time, providing a more comprehensive and precisely controlled experimental environment for studying the interaction of plant-soil systems under different environmental conditions, solving the problems of poor temperature and humidity regulation effect, inability to independently control soil humidity, and low utilization efficiency of isotope carbon dioxide in the prior art, helping to improve the accuracy and repeatability of the experiment and reduce the waste of experimental materials.

[0013] Preferably, an upper flange is fixedly connected to the outer side of the bottom of the upper box body, and a lower flange is fixedly connected to the outer side of the top of the lower box body, and the upper flange is detachably connected to the lower flange by bolts;

[0014] An upper sealing groove is provided at the center of the bottom surface of the upper flange, a lower sealing groove is provided at the center of the upper surface of the lower flange, and a sealing gasket is provided between the upper sealing groove and the lower sealing groove;

[0015] The present invention ensures the sealing of the marking chamber through the upper and lower flanges, the sealing gasket and the sealing groove, maintains the stability of the internal environment, reduces the risk of gas leakage, improves the accuracy of the experiment, facilitates the assembly and maintenance of the device, and reduces the cost.

[0016] Preferably, the material of the upper box and the lower box is a transparent acrylic sheet with a thickness of 20 mm;

[0017] The sealing gasket is made of butyl rubber.

[0018] The present invention utilizes a box body made of a 20 mm thick transparent acrylic sheet and a butyl rubber sealing gasket, which ensures structural stability and sealing, facilitates observation experiments, has chemical stability, reduces errors and failures caused by material performance degradation, and meets environmental protection requirements.

[0019] Preferably, the concentration control component includes a first fixed plate, one end of the first fixed plate is fixedly connected to the upper box body, one end of the first fixed plate away from the upper box body is detachably connected to the first three-way valve, one end of the first three-way valve close to the upper box body is connected to the first connecting pipe, one end of the first connecting pipe away from the first three-way valve is connected to the upper box body, one end of the first three-way valve away from the first connecting pipe is connected to the first washing pipe, the bottom end of the first washing pipe is connected to the first washing bottle, the top side of the first washing bottle is connected to the second washing pipe, the end of the second washing pipe away from the first washing bottle is connected to the second washing bottle, the top side of the second washing bottle is connected to the third washing pipe, and the end of the third washing pipe away from the second washing bottle is provided with a first suction pump. The first suction pump in the present invention can be selected from KLZP6.

[0020] The present invention can achieve high-precision regulation of the concentration of carbon dioxide entering the device through multi-stage washing and precise gas flow control. Researchers can accurately set and maintain the gas environment in the device according to experimental requirements, providing a powerful means for in-depth research on the interaction of plant-soil systems under different gas concentration conditions.

[0021] Preferably, a sodium hydroxide solution is provided inside the first gas washing bottle;

[0022] The interior of the second gas washing bottle is provided with a calcium hydroxide solution.

[0023] In the present invention, sodium hydroxide is a strong alkali, and its solution can efficiently absorb acidic components in the gas, especially carbon dioxide. In the concentration control process, when the gas passes through the first gas washing bottle filled with sodium hydroxide solution, the hydroxide ions in the solution react chemically with the carbon dioxide to generate carbonate ions and water, thereby removing the carbon dioxide from the gas, achieving the purpose of purifying the gas and reducing the carbon dioxide concentration, providing a gas environment with a low carbon dioxide concentration for subsequent experiments, and meeting the requirements of specific experiments on gas components.

[0024] The calcium hydroxide solution in the present invention also has a certain alkalinity and can further react with the acidic impurities in the gas. After the initial treatment by the first gas washing bottle, a small amount of acidic components or other impurities may still remain in the gas. The calcium ions and hydroxide ions in the calcium hydroxide solution can react chemically with these impurities to generate precipitation or other harmless substances, further purify the gas, improve the purity and quality of the gas, ensure that the gas components entering the device are purer and more stable, provide a more accurate gas environment for the experiment, and reduce the interference of impurities on the experimental results.

[0025] Preferably, the concentration monitoring assembly includes a second fixed plate, one end of the second fixed plate is fixedly connected to the upper box body, one end of the second fixed plate away from the upper box body is detachably connected to the second three-way valve, one end of the second three-way valve close to the upper box body is connected to the second connecting pipe, one end of the second connecting pipe away from the second three-way valve is connected to the upper box body, one end of the second three-way valve away from the second connecting pipe is connected to the first circulation pipe, one end of the first circulation pipe away from the second three-way valve is provided with an infrared gas analyzer, the top of the second three-way valve is connected to the second circulation pipe, and one end of the second circulation pipe away from the second three-way valve is connected to the infrared gas analyzer. In the present invention, the infrared gas analyzer can be selected from GXH-3010E1.

[0026] The present invention utilizes an infrared gas analyzer to perform circulation monitoring on the gas in the device. Through the design of the second three-way valve and the circulation pipe, the change of the gas concentration in the device can be detected in real time and accurately, and the change is fed back to the control system so as to timely adjust the working status of the concentration control component and other related components, thereby ensuring that the gas concentration in the device is always maintained within the set range, providing stable and accurate gas concentration conditions for the experiment, and helping to improve the accuracy and repeatability of the experiment.

[0027] Preferably, the humidification component includes a third fixed plate, which is fixedly connected to the inner side of the upper box body, a fixed ring is fixedly connected to the bottom end of the third fixed plate, a humidification tube is arranged on the inner side of the fixed ring, a point nozzle is arranged at one end of the humidification tube, an ultrasonic humidifier is arranged at the end of the humidification tube away from the point nozzle, the end of the third fixed plate away from the upper box body is rotatably connected to a rotating column, an electric knob is arranged at the top of the rotating column, a guide rail is fixedly connected to the side of the rotating column, an electric slide is slidably connected to the inner side of the guide rail, a smooth circular hole is opened at the bottom end of the book searching electric slide, and the humidification tube passes through the electric slide along the smooth circular hole.

[0028] The present invention utilizes an ultrasonic humidifier to generate water vapor, which is then evenly sprayed into a culture bowl through a humidifying tube and a point nozzle. The design of an electric knob and an electric slide can flexibly adjust the position and direction of the humidifying tube, thereby achieving precise control of the humidity in the device and reducing the impact on other structures when humidification is not required, thereby helping to improve the accuracy and scientificity of the experiment and solving the problem of poor humidity regulation effect in the prior art.

[0029] Preferably, the dehumidification assembly includes a fourth fixing plate, one end of the fourth fixing plate is fixedly connected to the upper box body, a first ventilation valve is arranged below the fourth fixing plate, an end of the first ventilation valve close to the upper box body is connected to a third connecting pipe, an end of the third connecting pipe away from the first ventilation valve is connected to the upper box body, an end of the first ventilation valve away from the third connecting pipe is connected to a first drying pipe, an end of the first drying pipe away from the first ventilation valve is connected to a drying ball, and an end of the drying ball away from the first drying pipe is connected to a second air pump; the second air pump in the present invention can be selected from KLZP6;

[0030] A second drying pipe is arranged below the first drying pipe, one end of the second drying pipe is connected to the drying ball, a second ventilation valve is arranged at the end of the second drying pipe away from the drying ball, a fifth fixing plate is arranged above the second ventilation valve, one side of the fifth fixing plate is fixedly connected to the lower box body, a fourth connecting pipe is connected to the end of the second ventilation valve away from the second drying pipe, and an end of the fourth connecting pipe away from the second ventilation valve is connected to the lower box body.

[0031] The present invention extracts gas from the device through a second vacuum pump and passes it through a drying ball for dehumidification treatment. The design of the first ventilation valve, the second ventilation valve and the two drying tubes can flexibly control the dehumidification process and effect, and can effectively reduce the humidity in the device. When used in conjunction with a humidifying component, precise adjustment of the humidity is achieved, providing a stable humidity environment for the experiment, helping to improve the accuracy and reliability of experimental data, solving the problem of unsatisfactory humidity adjustment in the prior art, and also avoiding interference with experimental results due to excessive moisture.

[0032] Preferably, the air pressure balancing assembly includes a sixth fixed plate, one end of which is fixedly connected to the upper box body, a pneumatic balancing valve is arranged on the side of the sixth fixed plate, a ventilation pipe is connected to the side of the pneumatic balancing valve close to the upper box body, the end of the ventilation pipe away from the pneumatic balancing valve is connected to the upper box body, an air isolation frame is arranged on the side of the pneumatic balancing valve away from the ventilation pipe, an air isolation frame is connected to the side of the pneumatic balancing valve away from the air isolation frame, a gas supply pipe is connected to the end of the gas supply pipe away from the gas isolation frame, and a gas sampling bag is arranged at the bottom end of the rotary valve.

[0033] The present invention utilizes a pneumatic balancing valve to sense and adjust the air pressure in the device in real time, thereby maintaining the air pressure stable and avoiding the influence of air pressure changes on the experiment, which helps to accurately study the relevant characteristics and change laws of the gas in the device and provide reliable data support for the experiment.

[0034] Preferably, the gas sampling bag is made of aluminum foil.

[0035] The gas sampling bag made of aluminum foil in the present invention has excellent barrier properties, can effectively prevent gas leakage and mixing of external gas, ensure that the collected gas samples have high purity and accuracy, provide reliable samples for subsequent gas composition analysis, isotope detection and other experiments, help to improve the credibility and scientificity of experimental results, ensure the authenticity and validity of experimental data, and meet the high-precision requirements for gas research in plant-soil systems.

[0036] Preferably, a transmission column is rotatably connected to the side of the air isolation frame, a pointer is fixedly connected to the side of the transmission column, and an angle sensor is provided on the side of the pointer away from the air isolation frame.

[0037] Through the cooperation of the transmission column, the pointer and the angle sensor, the present invention can monitor the rotation of the air baffle in real time, indirectly reflect the gas flow and pressure changes in the gas sampling process, and provide feedback information for the precise control of the air pressure balance component, which is helpful to further optimize the adjustment effect of the air pressure balance, ensure the stability of the air pressure in the device, and improve the stability and reliability of the experimental environment. At the same time, it also provides a guarantee for the accuracy of gas sampling, ensuring that the collected gas samples can truly reflect the gas state in the device.

[0038] Preferably, a first rotating disk and a second rotating disk are fixedly connected to one side of the transmission column close to the air isolation frame, and an air isolation plate is arranged between the first rotating disk and the second rotating disk.

[0039] The present invention utilizes a gas separation plate to separate the gases on both sides, thereby reducing gas mixing and improving the accuracy and reliability of gas sampling.

[0040] Preferably, both ends of the air separation plate are in contact with the inner wall of the air separation frame, and both sides of the air separation plate are respectively in contact with the first rotating disk and the second rotating disk. The surface of the air separation plate is smooth, and the bottom surface of the second rotating disk is in contact with the inner wall of the air separation frame.

[0041] The present invention minimizes gas leakage and cross-flow between the gas baffle and surrounding components through a tight fit and smooth surface design, ensuring that the gas can flow along a predetermined path, improving the working efficiency of the gas pressure balance component and the accuracy of gas sampling, providing stable and reliable gas samples for the experiment, ensuring the accuracy and scientific nature of the experimental results, and also helping to extend the service life of the device and reduce maintenance costs.

[0042] Preferably, the heating assembly includes a controller, which is arranged at the top center of the upper box body, and an electric heating plate is arranged below the controller.

[0043] The controller in the present invention can accurately control the working state of the ceramic electric heating plate according to preset temperature parameters, providing precise temperature conditions for studying the response of the plant-soil system under different temperature environments, helping to improve the accuracy and repeatability of the experiment, and solving the problem of poor temperature regulation effect in the prior art.

[0044] Preferably, the electric heating plate is made of ceramic. In the present invention, the electric heating plate can be a PTC electric heater.

[0045] The ceramic electric heating plate in the present invention has good thermal conductivity and high temperature resistance, can efficiently convert electrical energy into thermal energy, and quickly increase the temperature in the device. At the same time, its stable chemical properties ensure that it will not chemically react with the gas or other substances in the device during the heating process, thereby ensuring the purity and stability of the experimental environment, providing reliable temperature conditions for the experiment, and helping to accurately study the physiological and ecological processes of the plant-soil system under a specific temperature environment, thereby improving the credibility and scientificity of the experimental results.

[0046] Preferably, the heat dissipation assembly includes a sealing base, which is fixedly connected to the side wall of the lower box body, a heat sink is arranged on the inner side of the sealing base, a heat dissipation base is arranged on the middle side of the sealing base, and a heat dissipation fan is arranged on the side of the heat dissipation base away from the sealing base.

[0047] When the heat dissipation fan in the present invention is running, it will accelerate the flow of air near the side wall of the lower box body, so that heat can be dissipated from the heat sink to the surrounding environment more quickly. Since the heating component is installed on the top of the upper box body, and the upper box body is not provided with a structure for heat dissipation, based on the principle of thermal expansion and contraction and buoyancy, the hot air naturally rises in the marking room, which causes the temperature in the marking room to change with the height, with the top temperature of the upper box body being the highest and the bottom temperature of the lower box body being the lowest. In addition, the sealing base ensures the sealing between the heat dissipation component and the lower box body, prevents external dust and impurities from entering the interior of the device, and also helps to maintain the air pressure in the device stable. The present invention can effectively adjust the temperature in the device, provide a stable temperature environment for the experiment, ensure the smooth progress of the experiment, and avoid adverse effects on experimental equipment and experimental results due to excessive temperature.

[0048] In addition, the upper and lower boxes are made of acrylic panels, which have relatively weak thermal conductivity, which is conducive to maintaining a relatively independent thermal environment inside the marking room and helps to form a temperature difference.

[0049] Furthermore, in order to better achieve temperature control, the device should be placed in a temperature-adjustable laboratory for use to reduce the impact of external ambient temperature on the device and ensure the effectiveness of temperature regulation.

[0050] Preferably, the regulating component includes a rotating platform, which is arranged below the tray, and the bottom of the tray is detachably connected to the upper surface of the rotating platform. A telescopic rod is arranged below the edge of the rotating platform, and a lifting column is rotatably connected to the center below the rotating platform. A fixing frame is arranged on the side of the lifting column, and a ball screw is arranged on the inner side of the lifting column. The bottom end side of the ball screw is rotatably connected to the support frame, and a first bevel gear is fixedly connected below the bottom end of the ball screw, and the side of the first bevel gear is meshingly connected to the second bevel gear, and a servo motor is arranged on the side of the second bevel gear away from the first bevel gear, and the output end of the servo motor is fixedly connected to the second bevel gear.

[0051] In the present invention, the servo motor drives the second bevel gear to rotate through the first bevel gear, thereby driving the ball screw to rotate, and further driving the lifting column to rise or fall along the ball screw, thereby adjusting the height of the rotating platform, that is, changing the height of the culture pot, and the rotating platform can be rotated relative to the lifting column, and the design of the telescopic rod, the fixed frame, the support frame, etc. is conducive to enhancing the stability of the structure. The present invention facilitates researchers to install and disassemble the culture pot by quickly adjusting the height of the culture pot. Since the temperature at the high point in the marking room is generally higher than the temperature at the low point, the temperature around the plant can also be changed to a certain extent by changing the height during the experiment, thereby increasing the diversity of the experiment. Furthermore, when a fixed light source is used, the lighting environment of the plant may also be changed, thereby expanding the research depth and breadth of the experiment.

[0052] Preferably, the top end of the telescopic rod is connected to the rotating platform, the bottom end of the telescopic rod is fixedly connected to a transmission plate, a stepping turntable is fixedly connected below the transmission plate, a driving disk is arranged on the side of the stepping turntable, a driving column is fixedly connected to the lower edge of the driving disk, a driving plate is fixedly connected to the lower middle part of the driving disk, a stepping motor is arranged below the driving plate, and the output end of the stepping motor is fixedly connected to the driving plate.

[0053] In the present invention, the stepper motor drives the driving disk to rotate 90° each time it runs, thereby using the driving column to drive the driving turntable to rotate 60°, and then driving the telescopic column to rotate 60° through the transmission plate, and using the rotating platform to rotate the culture pot 60° in the marking room, so that the culture pot can be changed in position on the horizontal plane, which is convenient for installation and disassembly, and is also beneficial for adjusting the lighting environment of the plant. It is also convenient to cooperate with the humidification component, and the flexibility of the device is enhanced. There is no need to set a fixed humidification tube for each culture pot, so as to avoid interference of too many humidification tubes on the experiment.

[0054] Preferably, a groove and a slide groove are provided on the edge of the stepping turntable, the size of the groove is adapted to the driving plate, and the size of the slide groove is adapted to the driving column;

[0055] A through hole is provided at the center of the stepping turntable, and the ball screw passes through the stepping turntable along the through hole.

[0056] The grooves and slides are used to drive the drive disc to stably and accurately drive the stepping turntable to rotate, thereby driving the rotating platform to rotate, ensuring the accuracy and reliability of the control component. The setting of the through hole facilitates the installation and movement of the ball screw, avoiding the stepping turntable from affecting the movement of the ball screw, making the entire control component more compact and coordinated, reducing space occupancy, providing a more stable and precise culture bowl position control for the experiment, and ensuring the accuracy and repeatability of the experimental results.

[0057] Preferably, a first protective shell is provided on the outer side of the servo motor, the bottom end of the first protective shell is detachably connected to the lower box body, and the bottom end of the fixing frame is detachably connected to the upper surface of the first protective shell.

[0058] The first protective shell can effectively protect the servo motor from interference and damage by external factors, ensure the normal operation of the servo motor, and support the fixed frame, thereby ensuring the stable operation of the regulating component.

[0059] Preferably, a second protective shell is provided on the outer side of the stepper motor, the bottom end of the second protective shell is detachably connected to the lower box body, and the upper surface of the second protective shell is in contact with the bottom surface of the driving plate.

[0060] The second protective shell protects the stepper motor, preventing dust and water vapor from entering the motor and affecting its performance and life. The detachable connection with the lower box and the fitting design with the driver board make the installation of the stepper motor more stable, and provide support for the driver board, ensuring the accuracy and reliability of the control components, providing stable conditions for the experiment, and helping to improve the accuracy and scientificity of the experimental data.

[0061] The second technical solution of the present invention: temperature and humidity control 13 The method for manufacturing a C continuous stable isotope labeling device comprises the following steps:

[0062] (S01) preparing a transparent acrylic sheet with a thickness of 20 mm, making the overall frames of the upper box and the lower box respectively, installing an upper flange on the outer side of the bottom of the upper box, and installing a lower flange on the outer side of the top of the lower box;

[0063] (S02) installing a diaphragm pressure gauge on the top edge of the upper box, installing a heating component at the top center of the upper box, installing a regulating component on the inner side of the lower box, and installing a heat dissipation component on the side wall of the lower box;

[0064] (S03) Pass the humidification tube through the fixing ring and the smooth circular hole, install the point nozzle on the humidification tube, and then install the humidification assembly as a whole on the side of the upper box body;

[0065] (S04) placing a sealing gasket between the upper flange and the lower flange, and then connecting the upper flange and the lower flange together using bolts to form a marking chamber;

[0066] (S05) The concentration control component, the concentration monitoring component, the dehumidification component and the air pressure balance component are sequentially installed on the side of the marking chamber, and silicone rubber is used to further reinforce the seal at all interfaces of the device. At this point, the device is completed.

[0067] The silicone rubber in the present invention can be selected from Nanda 704 silicone rubber.

[0068] The present invention ensures the structural integrity of the device by cooperating with each other through the various components, and also uses silicone rubber to reinforce and seal the interfaces, further enhancing the airtightness of the device and preventing gas leakage, thereby providing a guarantee for the stable control of the environment within the device, helping to improve the accuracy and reliability of the experiment, and solving the problems existing in the prior art such as poor temperature and humidity regulation effect, unsuitability for repeated experiments, and waste of experimental materials, thereby providing an effective tool for studying the interaction of plant-soil systems under precisely controlled environmental conditions.

[0069] The present invention has the following beneficial effects:

[0070] (1) The device achieves precise and comprehensive control of multiple environmental factors such as gas concentration, temperature, humidity, and air pressure. The concentration control component uses a multi-stage washing process to accurately control the gas concentration; the humidification and dehumidification components work together to flexibly adjust the humidity; the heating component and the heat dissipation component work together to stabilize the temperature, creating an ideal and precise experimental environment for studying the interaction of plant-soil systems under different environmental conditions, and effectively solving the problem of poor temperature and humidity regulation in existing technologies.

[0071] (2) The height and horizontal position of the culture pot can be quickly adjusted. This makes it easier for researchers to install and disassemble the culture pot, flexibly change the lighting environment of the plants, and enhance the flexibility of coordination with the humidification component. At the same time, the infrared gas analyzer can accurately monitor the gas concentration in real time, and the digital soil thermometer and hygrometer on the side of the culture pot can monitor the temperature and humidity conditions, which helps to maintain a stable experimental environment, greatly improves the accuracy and repeatability of the experiment, reduces the waste of experimental materials, and ensures the reliability of experimental data quality.

[0072] (3) The good sealing of the marking room is ensured, the risk of gas leakage is effectively reduced, and the internal environment is maintained stable, so that the various environmental control functions can be fully utilized, the service life of the device is extended, and the maintenance cost is reduced, so that it can serve various experimental researches stably in the long term.

[0073] (4) Real-time sensing and adjustment of the gas pressure inside the device to reduce gas mixing and leakage, achieve accurate gas sampling, and ensure that the collected gas samples have high purity and accuracy, meet the high-precision requirements for gas research in plant-soil systems, and provide reliable guarantees for subsequent experimental analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0074] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0075] Figure 2 It is a schematic diagram of the upper box body of the present invention;

[0076] Figure 3 is a schematic diagram of the upper flange of the present invention;

[0077] Figure 4 is a schematic diagram of the lower flange of the present invention;

[0078] Figure 5 is a schematic diagram of a sealing gasket of the present invention;

[0079] Figure 6 It is a schematic diagram of the pressure of the diaphragm box of the present invention;

[0080] Figure 7 is a schematic diagram of a concentration control assembly of the present invention;

[0081] Figure 8is a schematic diagram of a concentration monitoring assembly of the present invention;

[0082] Fig. 9 is a schematic diagram of a humidifying assembly of the present invention;

[0083] Fig.10 is a schematic diagram of the disassembly of the humidification component of the present invention;

[0084] Fig.11 is a schematic diagram of a dehumidification assembly of the present invention;

[0085] Fig.12 is a schematic diagram of the air pressure balance assembly of the present invention;

[0086] Fig.13 is a schematic diagram of an air isolation frame of the present invention;

[0087] Fig.14 is a schematic diagram of an air barrier plate of the present invention;

[0088] Fig.15 is a schematic diagram of a heating assembly of the present invention;

[0089] Fig.16 is a schematic diagram of a heat dissipation assembly of the present invention;

[0090] Fig.17 is a schematic diagram of a sealing base of the present invention;

[0091] Fig.18 is a schematic diagram of the regulatory components of the present invention;

[0092] Fig.19 is a side schematic diagram of the control component of the present invention;

[0093] Fig. 20 is a schematic diagram of disassembling the control component of the present invention;

[0094] Fig.21 is a schematic diagram of a ball screw of the present invention;

[0095] Fig. 22 is a schematic diagram of a stepping turntable of the present invention;

[0096] Fig.23 is a schematic diagram of a servo motor of the present invention;

[0097] Fig.24 It is a schematic diagram of a driving board of the present invention.

[0098] The marks in the attached drawings are: 100, marking chamber; 200, concentration control component; 300, concentration monitoring component; 400, humidification component; 500, dehumidification component; 600, air pressure balance component; 700, membrane box pressure gauge; 800, heating component; 900, heat dissipation component; 103, regulation component; 104, culture pot; 105, digital display soil temperature and humidity meter; 1041, tray; 101, upper box; 1011, upper flange; 10111, upper sealing groove; 102, lower box; 1021, lower flange; 10121, lower sealing groove; 201, first fixing plate; 202, first three-way valve; 2021, first connecting pipe; 203, first gas washing pipe; 204 , first gas washing bottle; 205, second gas washing pipe; 206, second gas washing bottle; 207, third gas washing pipe; 208, first vacuum pump; 301, second fixed plate; 302, second three-way valve; 3021, second connecting pipe; 303, first circulation pipe; 304, infrared gas analyzer; 305, second circulation pipe; 401, third fixed plate; 4011, fixed ring; 402, humidification pipe; 4021, point nozzle; 403, ultrasonic humidifier; 404, rotating column; 4041, electric knob; 405, guide rail; 4051, electric slide; 4052, smooth round hole; 501, fourth fixed plate; 502, first ventilation valve; 5021, third connecting tube; 503, first drying tube; 504, drying ball; 505, second vacuum pump; 506, second drying tube; 507, second ventilation valve; 5071, fourth connecting tube; 508, fifth fixing plate; 601, sixth fixing plate; 602, pneumatic balance valve; 6021, ventilation tube; 603, air separation frame; 6031, transmission column; 60311, pointer; 6032, angle sensor; 6033, first rotating disk; 6034, second rotating disk; 6035, air separation plate; 605, gas sampling bag; 6051, rotary valve; 801, controller; 802, electric heating plate; 901, sealing base; 902, heat sink; 903, heat dissipation base; 9 04, cooling fan; 1031, rotating platform; 1032, telescopic rod; 10321, transmission plate; 10322, stepping turntable; 103221, groove; 103222, slide groove; 103223, through hole; 10323, driving disk; 10324, driving column; 10325, driving plate; 10326, stepping motor; 103261, second protective shell; 1033, lifting column; 1034, fixing frame; 1035, ball screw; 10351, first bevel gear; 10352, second bevel gear; 10353, servo motor; 103531, first protective shell; 1013, sealing gasket; 1036, supporting frame; 604, air pipe. DETAILED DESCRIPTION

[0099] The present invention is further described below in conjunction with the accompanying drawings and embodiments, but they are not intended to limit the present invention.

[0100] Temperature and humidity control 13 C continuous stable isotope labeling device, including Figure 1 The marking chamber 100 shown in FIG. 1 has a side provided with a Figure 7 The concentration control component 200 shown in FIG. 1 is provided with a Figure 8 The concentration monitoring component 300 shown in FIG. 1 is provided with a side of the concentration monitoring component 300 away from the concentration control component 200. Fig. 9 The humidifying component 400 shown in FIG. 1 is provided with a humidifying component 400 on one side away from the concentration monitoring component 300. Fig.11 The dehumidification component 500 shown in FIG. 5 is provided with a side of the dehumidification component 500 away from the humidification component 400. Fig.12 The air pressure balance assembly 600 is shown;

[0101] The marking chamber 100 includes Figure 2 The upper box body 101 shown has a lower box body 102 disposed below the upper box body 101;

[0102] The top edge of the upper box 101 is provided with Figure 6 The diaphragm pressure gauge 700 shown in FIG. 1 has a top center of the upper box 101 provided with a Fig.15 The heating assembly 800 shown in the figure has a side wall of the lower box 102 provided with a Fig.16 The heat dissipation assembly 900 is shown;

[0103] A regulating component 103 is disposed inside the lower box 102, a culture pot 104 is disposed above the regulating component 103, and a digital display soil temperature and humidity meter 105 is disposed on the side of the culture pot 104;

[0104] A tray 1041 is provided below the culture pot 104 .

[0105] The bottom outer side of the upper box body 101 is fixedly connected as shown in FIG. Figure 3 The upper flange 1011 shown in the figure is fixedly connected to the top outer side of the lower box body 102 as shown in the figure. Figure 4 The lower flange 1021 shown, the upper flange 1011 is detachably connected to the lower flange 1021 by bolts;

[0106] An upper sealing groove 10111 is provided at the center of the bottom surface of the upper flange 1011, a lower sealing groove 10121 is provided at the center of the upper surface of the lower flange 1021, and a sealing groove 10111 is provided between the upper sealing groove 10121. Figure 5 Sealing gasket 1013 shown;

[0107] The material of the upper box 101 and the lower box 102 is a transparent acrylic sheet with a thickness of 20 mm;

[0108] The material of the sealing gasket 1013 is butyl rubber.

[0109] The concentration control assembly 200 includes a first fixed plate 201, one end of the first fixed plate 201 is fixedly connected to the upper box body 101, the end of the first fixed plate 201 away from the upper box body 101 is detachably connected to a first three-way valve 202, the end of the first three-way valve 202 close to the upper box body 101 is connected to a first connecting pipe 2021, the end of the first connecting pipe 2021 away from the first three-way valve 202 is connected to the upper box body 101, and the first three-way valve 202 is away from the first connecting pipe One end of 2021 is connected to the first gas washing pipe 203, the bottom end of the first gas washing pipe 203 is connected to the first gas washing bottle 204, the top side of the first gas washing bottle 204 is connected to the second gas washing pipe 205, the end of the second gas washing pipe 205 away from the first gas washing bottle 204 is connected to the second gas washing bottle 206, the top side of the second gas washing bottle 206 is connected to the third gas washing pipe 207, and the end of the third gas washing pipe 207 away from the second gas washing bottle 206 is provided with a first vacuum pump 208.

[0110] The first gas washing bottle 204 is provided with a sodium hydroxide solution inside;

[0111] The second gas washing bottle 206 is provided with a calcium hydroxide solution therein.

[0112] The concentration monitoring component 300 includes a second fixed plate 301, one end of the second fixed plate 301 is fixedly connected to the upper box body 101, the end of the second fixed plate 301 away from the upper box body 101 is detachably connected to the second three-way valve 302, the end of the second three-way valve 302 close to the upper box body 101 is connected to the second connecting pipe 3021, the end of the second connecting pipe 3021 away from the second three-way valve 302 is connected to the upper box body 101, the end of the second three-way valve 302 away from the second connecting pipe 3021 is connected to the first circulation pipe 303, the end of the first circulation pipe 303 away from the second three-way valve 302 is provided with an infrared gas analyzer 304, the top of the second three-way valve 302 is connected to the second circulation pipe 305, and the end of the second circulation pipe 305 away from the second three-way valve 302 is connected to the infrared gas analyzer 304.

[0113] The humidification assembly 400 includes: Fig.10The third fixed plate 401 shown is fixedly connected to the inner side of the upper box body 101, and a fixing ring 4011 is fixedly connected to the bottom end of the third fixed plate 401, and a humidifying tube 402 is arranged on the inner side of the fixing ring 4011, and a point nozzle 4021 is arranged at one end of the humidifying tube 402, and an ultrasonic humidifier 403 is arranged at the end of the humidifying tube 402 away from the point nozzle 4021, and a rotating column 404 is rotatably connected to the end of the third fixed plate 401 away from the upper box body 101, and an electric knob 4041 is arranged at the top of the rotating column 404, and a guide rail 405 is fixedly connected to the side of the rotating column 404, and an electric slide 4051 is slidably connected to the inner side of the guide rail 405, and a smooth circular hole 4052 is opened at the bottom end of the book search electric slide 4051, and the humidifying tube 402 passes through the electric slide 4051 along the smooth circular hole 4052.

[0114] The dehumidification assembly 500 includes a fourth fixing plate 501, one end of the fourth fixing plate 501 is fixedly connected to the upper box body 101, a first ventilation valve 502 is arranged below the fourth fixing plate 501, one end of the first ventilation valve 502 close to the upper box body 101 is connected to a third connecting pipe 5021, one end of the third connecting pipe 5021 away from the first ventilation valve 502 is connected to the upper box body 101, one end of the first ventilation valve 502 away from the third connecting pipe 5021 is connected to a first drying pipe 503, one end of the first drying pipe 503 away from the first ventilation valve 502 is connected to a drying ball 504, and one end of the drying ball 504 away from the first drying pipe 503 is connected to a second air pump 505;

[0115] A second drying tube 506 is arranged below the first drying tube 503, one end of the second drying tube 506 is connected to the drying ball 504, a second ventilation valve 507 is arranged at the end of the second drying tube 506 away from the drying ball 504, a fifth fixing plate 508 is arranged above the second ventilation valve 507, one side of the fifth fixing plate 508 is fixedly connected to the lower box body 102, an end of the second ventilation valve 507 away from the second drying tube 506 is connected to a fourth connecting tube 5071, and an end of the fourth connecting tube 5071 away from the second ventilation valve 507 is connected to the lower box body 102.

[0116] The air pressure balancing assembly 600 includes a sixth fixed plate 601, one end of the sixth fixed plate 601 is fixedly connected to the upper box body 101, a pneumatic balancing valve 602 is arranged on the side of the sixth fixed plate 601, a ventilation pipe 6021 is connected to the side of the pneumatic balancing valve 602 close to the upper box body 101, the end of the ventilation pipe 6021 away from the pneumatic balancing valve 602 is connected to the upper box body 101, an air isolation frame 603 is arranged on the side of the pneumatic balancing valve 602 away from the ventilation pipe 6021, an air isolation frame 603 is connected to the side of the air isolation frame 603 away from the pneumatic balancing valve 602, an air supply pipe 604 is connected to the end of the air supply pipe 604 away from the air isolation frame 603, a rotary valve 6051 is connected to the bottom end of the rotary valve 6051.

[0117] The material of the gas sampling bag 605 is aluminum foil.

[0118] like Fig.13 The side of the air isolation frame 603 is rotatably connected to a transmission column 6031 , the side of the transmission column 6031 is fixedly connected to a pointer 60311 , and an angle sensor 6032 is provided on the side of the pointer 60311 away from the air isolation frame 603 .

[0119] A first rotating disk 6033 and a second rotating disk 6034 are fixedly connected to one side of the transmission column 6031 close to the air isolation frame 603 , and an air isolation plate 6035 is disposed between the first rotating disk 6033 and the second rotating disk 6034 .

[0120] like Fig.14 The two ends of the air isolation plate 6035 shown are in contact with the inner wall of the air isolation frame 603, and the two sides of the air isolation plate 6035 are respectively in contact with the first rotating disk 6033 and the second rotating disk 6034. The surface of the air isolation plate 6035 is smooth, and the bottom surface of the second rotating disk 6034 is in contact with the inner wall of the air isolation frame 603.

[0121] The heating assembly 800 includes a controller 801 , which is disposed at the top center of the upper box 101 , and an electric heating plate 802 is disposed below the controller 801 .

[0122] The material of the electric heating plate 802 is ceramic.

[0123] The heat dissipation assembly 900 includes: Fig.17 The sealing base 901 shown is fixedly connected to the side wall of the lower box body 102, a heat sink 902 is arranged on the inner side of the sealing base 901, a heat dissipation base 903 is arranged on one side of the middle part of the sealing base 901, and a heat dissipation fan 904 is arranged on the side of the heat dissipation base 903 away from the sealing base 901.

[0124] like Fig.18 The control assembly 103 shown includes a rotating platform 1031, such as Fig.19The rotating platform 1031 shown is arranged below the tray 1041, the bottom of the tray 1041 is detachably connected to the upper surface of the rotating platform 1031, and a Fig. 20 The telescopic rod 1032 shown in the figure is rotatably connected to a lifting column 1033 at the center below the rotating platform 1031. A fixing frame 1034 is arranged on the side of the lifting column 1033. Fig.21 The ball screw 1035 shown in the figure has a support frame 1036 rotatably connected to the side of the bottom end of the ball screw 1035, a first bevel gear 10351 is fixedly connected to the bottom of the bottom end of the ball screw 1035, a second bevel gear 10352 is meshedly connected to the side of the first bevel gear 10351, and a side of the second bevel gear 10352 away from the first bevel gear 10351 is provided with Fig.23 The servo motor 10353 shown has an output end fixedly connected to the second bevel gear 10352 .

[0125] The top of the telescopic rod 1032 is connected to the rotating platform 1031, the bottom of the telescopic rod 1032 is fixedly connected to the transmission plate 10321, and the bottom of the transmission plate 10321 is fixedly connected to the rotating platform 1031. Fig. 22 The stepping turntable 10322 shown in the figure has a driving disk 10323 disposed on the side of the stepping turntable 10322, a driving column 10324 is fixedly connected to the lower edge of the driving disk 10323, and a driving column 10324 is fixedly connected to the lower middle part of the driving disk 10323. Fig.24 As shown in the driving board 10325 , a stepper motor 10326 is arranged below the driving board 10325 , and the output end of the stepper motor 10326 is fixedly connected to the driving board 10325 .

[0126] The edge of the stepping turntable 10322 is provided with a groove 103221 and a slide groove 103222. The size of the groove 103221 is adapted to the driving plate 10325, and the size of the slide groove 103222 is adapted to the driving column 10324.

[0127] A through hole 103223 is provided at the center of the stepping turntable 10322 , and the ball screw 1035 passes through the stepping turntable 10322 along the through hole 103223 .

[0128] A first protective shell 103531 is disposed outside the servo motor 10353 , the bottom end of the first protective shell 103531 is detachably connected to the lower box body 102 , and the bottom end of the fixing bracket 1034 is detachably connected to the upper surface of the first protective shell 103531 .

[0129] A second protective shell 103261 is provided on the outer side of the stepper motor 10326 , the bottom end of the second protective shell 103261 is detachably connected to the lower box body 102 , and the upper surface of the second protective shell 103261 is in contact with the bottom surface of the driving plate 10325 .

[0130] A method for manufacturing a temperature and humidity controlled 13C continuous stable isotope labeling device comprises the following steps:

[0131] (S01) Prepare a transparent acrylic sheet with a thickness of 20 mm, respectively make the overall frame of the upper box 101 and the lower box 102, install the upper flange 1011 on the outer side of the bottom of the upper box 101, and install the lower flange 1021 on the outer side of the top of the lower box 102;

[0132] (S02) Installing a diaphragm pressure gauge 700 on the top edge of the upper box 101, installing a heating component 800 at the top center of the upper box 101, installing a regulating component 103 on the inner side of the lower box 102, and installing a heat dissipation component 900 on the side wall of the lower box 102;

[0133] (S03) Pass the humidifying tube 402 through the fixing ring 4011 and the smooth circular hole 4052, and install the point nozzle 4021 on the humidifying tube 402, and then install the humidifying assembly 400 as a whole on the side of the upper box 101;

[0134] (S04) placing a sealing gasket 1013 between the upper flange 1011 and the lower flange 1021, and then connecting the upper flange 1011 and the lower flange 1021 together using bolts to form the marking chamber 100;

[0135] (S05) The concentration control component 200, the concentration monitoring component 300, the dehumidification component 500 and the air pressure balance component 600 are sequentially installed on the side of the marking chamber 100, and silicone rubber is used to further reinforce the seal at all interfaces of the device. At this point, the device is completed.

[0136] The working principle of the present invention is:

[0137] The present invention utilizes an upper box body 101 and a lower box body 102 made of a transparent acrylic plate with a thickness of 20 mm, which are connected via an upper flange 1011, a lower flange 1021 and a sealing gasket 1013 to form a marking chamber 100, providing a relatively closed and stable space foundation for the entire experimental environment, while also facilitating observation of the experimental conditions and having chemical stability, thereby reducing problems caused by degradation of material properties and meeting environmental protection requirements.

[0138] The concentration control component 200 is connected to the upper box 101 through the first fixed plate 201. The gas enters the first gas washing bottle 204 (containing sodium hydroxide solution) from the device through the first connecting pipe 2021 and the first three-way valve 202 in sequence. The hydroxide ions in the sodium hydroxide solution react chemically with acidic components such as carbon dioxide in the gas to remove carbon dioxide, thereby achieving the purpose of purifying the gas and reducing the concentration of carbon dioxide.

[0139] The gas then enters the second gas washing bottle 206 (containing calcium hydroxide solution) through the second gas washing pipe 205. The calcium hydroxide solution further reacts with the residual acidic impurities or other impurities to generate precipitates or harmless substances, further purifying the gas and improving the gas purity.

[0140] Finally, the gas is extracted from the concentration control component 200 through the third gas washing pipe 207 by the first vacuum pump 208, thereby achieving high-precision control of the concentration of the gas entering the device, meeting the requirements of different experiments on gas composition, and providing a precise gas environment for studying the interaction of the plant-soil system under specific gas concentrations.

[0141] The concentration monitoring component 300 is fixed to the upper box 101 by means of the second fixing plate 301. The gas passes through the second connecting pipe 3021 and the second three-way valve 302, and enters the infrared gas analyzer 304 through the first circulation pipe 303. The gas concentration is analyzed by using the absorption characteristics of infrared rays, and the data is transmitted to the control system. At the same time, the gas can also return to the marking chamber 100 again through the second circulation pipe 305. The gas circulates in the marking chamber 100 and the infrared gas analyzer 304 through the switching of the second three-way valve 302. The gas concentration change is monitored in real time and accurately, and is fed back to the control system so as to timely adjust the working state of the concentration control component 200 and other related components to ensure that the gas concentration in the device is always maintained within the set range, providing stable and accurate gas concentration conditions for the experiment.

[0142] The heating component 800 is located at the top center of the upper box 101. The controller 801 controls the operation of the ceramic electric heating plate 802. According to the preset temperature parameters, the electric heating plate 802 converts electrical energy into thermal energy, quickly raising the temperature in the marking chamber 100, and providing precise temperature conditions for studying the response of the plant-soil system under different temperature environments.

[0143] The heat dissipation assembly 900 is installed on the side wall of the lower box 102. When the temperature inside the device is too high, the heat dissipation fan 904 runs to accelerate the flow of air near the side wall of the lower box 102, so that the heat is dissipated from the heat sink 902 to the surrounding environment more quickly. Since the heating component 800 is at the top of the upper box 101 and the upper box 101 has no heat dissipation structure, based on the principles of thermal expansion and contraction and buoyancy, the hot air naturally rises in the marking chamber 100, so that the temperature in the marking chamber 100 changes with the height, the top temperature of the upper box 101 is the highest, and the bottom temperature of the lower box 102 is the lowest, thereby achieving effective regulation of the temperature in the device, providing a stable temperature environment for the experiment, and avoiding adverse effects on experimental equipment and experimental results due to excessive temperature. At the same time, the acrylic panels used in the upper box 101 and the lower box 102 have relatively weak thermal conductivity, which is conducive to maintaining a relatively independent thermal environment inside the marking chamber 100 and helps to form a temperature difference. The entire device is placed in a temperature-adjustable laboratory, which can further reduce the impact of the external ambient temperature on the device and ensure the effectiveness of temperature regulation.

[0144] The humidification component 400 is fixed to the inner side of the upper box body 101 by the third fixing plate 401. The ultrasonic humidifier 403 generates water vapor, which is accurately sprayed into the culture pot 104 through the humidification tube 402 and the point nozzle 4021. The electric knob 4041 and the electric slide 4051 can flexibly adjust the position and direction of the humidification tube 402 to enhance the accuracy of spraying and avoid excessive diffusion of water vapor, thereby meeting the requirements of plant growth and experimental research on humidity conditions.

[0145] The dehumidification component 500 is connected to the upper box body 101 by the fourth fixed plate 501, and the gas in the marking chamber 100 is extracted through the first ventilation valve 502 and the third connecting pipe 5021, and the gas is dehumidified through the drying ball 504. The design of the first drying tube 503, the second drying tube 506 and the second ventilation valve 507 can flexibly control the process and effect of dehumidification, and can effectively reduce the humidity in the device. When used in conjunction with the humidification component 400, it can achieve precise adjustment of the humidity, provide a stable humidity environment for the experiment, avoid interference with the experimental results due to excessive moisture, and help improve the accuracy and reliability of the experimental data.

[0146] The air pressure balance component 600 is fixed to the upper box body 101 by the sixth fixing plate 601. The pneumatic balance valve 602 is connected to the upper box body 101 through the ventilation pipe 6021. It can sense and adjust the air pressure in the device in real time, keep the air pressure stable, and avoid the influence of air pressure changes on the experiment. It is helpful to accurately study the relevant characteristics and change laws of the gas in the device, and provide reliable data support for the experiment. In terms of gas sampling, the gas enters the gas sampling bag 605 (made of aluminum foil) at the bottom of the rotary valve 6051 through the air isolation frame 603 and the gas transmission pipe 604. The aluminum foil material can effectively prevent gas leakage and the mixing of external gas, ensuring that the collected gas sample has high purity and accuracy. The transmission column 6031 and the pointer 60311 on the side of the air isolation frame 603 cooperate with the angle sensor 6032 to monitor the rotation of the air isolation plate 6035 in real time, indirectly reflecting the gas flow and pressure changes during the gas sampling process, which is the air pressure balance component 600. The precise control provides feedback information, which helps to further optimize the adjustment effect of the air pressure balance and ensure that the collected gas samples can truly reflect the gas state in the device. At the same time, the air isolation plate 6035 fits tightly with the inner wall of the air isolation frame 603 and the first rotating disk 6033 and the second rotating disk 6034 and has a smooth surface, which reduces gas leakage and crossflow and ensures that the gas can flow along a predetermined path, thereby improving the working efficiency of the air pressure balance component 600 and the accuracy of gas sampling, providing stable and reliable gas samples for the experiment, and ensuring the accuracy and scientificity of the experimental results.

[0147] The regulating component 103 is located inside the lower box 102, and its rotating platform 1031 is arranged below the tray 1041. The tray 1041 is used to place the culture pot 104. There is a digital soil temperature and humidity meter 105 on the side of the culture pot 104, which can monitor the soil temperature and humidity in real time. The servo motor 10353 drives the second bevel gear 10352 to rotate through the first bevel gear 10351, thereby driving the ball screw 1035 to rotate, and further driving the lifting column 1033 to rise or fall along the ball screw 1035, so as to adjust the height of the rotating platform 1031, that is, to change the height of the culture pot 104, and the rotating platform 1031 can rotate relative to the lifting column 1033. The designs of the telescopic rod 1032, the fixed frame 1034, and the supporting frame 1036 are conducive to enhancing the stability of the structure, which is convenient for researchers to install and disassemble the culture pot 104. At the same time, due to the temperature gradient in the marking chamber 100, the temperature around the plant is changed to a certain extent through the change of height, which increases the diversity of the experiment. When a fixed light source is used, the lighting environment of the plant may also be changed, which expands the depth and breadth of the experimental research.

[0148] In addition, each time the stepper motor 10326 operates, the driving disk 10323 is driven to rotate 90°, and the driving column 10324 is used to drive the stepper turntable 10322 to rotate 60°, and then the transmission plate 10321 drives the telescopic rod 1032 to rotate 60°, so that the rotating platform 1031 drives the culture pot 104 to rotate 60° in the marking chamber 100, so that the culture pot 104 can change its position on the horizontal plane, which is convenient for installation and disassembly, and is also beneficial for adjusting the lighting environment of the plant. It is also convenient to cooperate with the humidification component 400 to enhance the flexibility of the device. There is no need to set a fixed humidification tube 402 for each culture pot 104, so as to avoid interference with the experiment caused by too many humidification tubes 402. The groove 103221 and the slide groove 103222 of the stepper turntable 10322 are used to drive the disk 10323 stably and accurately. The stepping turntable 10322 is driven to rotate accurately, thereby driving the rotating platform 1031 to rotate, ensuring the accuracy and reliability of the control component 103. The through hole 103223 of the stepping turntable 10322 facilitates the installation and movement of the ball screw 1035, avoiding the stepping turntable 10322 from affecting the movement of the ball screw 1035, making the entire control component 103 more compact and coordinated, reducing space occupancy, providing a more stable and precise position control of the culture bowl 104 for the experiment, and ensuring the accuracy and repeatability of the experimental results. At the same time, the first protective shell 103531 of the servo motor 10353 and the second protective shell 103261 of the stepping motor 10326 respectively protect the motors to ensure the normal operation of the motors, thus providing a guarantee for the stable operation of the control component 103.

[0149] The present invention has been described in detail above by general description and specific embodiments, but it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. Temperature and humidity control 13 C continuous stable isotope labeling device, characterized by: The marking chamber (100) comprises a concentration control component (200) disposed on one side of the marking chamber (100), a concentration monitoring component (300) disposed on one side of the concentration control component (200), a humidifying component (400) disposed on a side of the concentration monitoring component (300) away from the concentration control component (200), a dehumidifying component (500) disposed on a side of the humidifying component (400) away from the concentration monitoring component (300), and a pressure balancing component (600) disposed on a side of the dehumidifying component (500) away from the humidifying component (400); The marking chamber (100) comprises an upper box body (101), and a lower box body (102) is arranged below the upper box body (101); A diaphragm pressure gauge (700) is provided on the top edge of the upper box body (101), a heating component (800) is provided at the top center of the upper box body (101), and a heat dissipation component (900) is provided on the side wall of the lower box body (102); A regulating component (103) is arranged on the inner side of the lower box (102), a culture pot (104) is arranged above the regulating component (103), and a digital display soil temperature and humidity meter (105) is arranged on the side of the culture pot (104); A tray (1041) is provided below the culture pot (104).

2. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: An upper flange (1011) is fixedly connected to the outer side of the bottom of the upper box body (101), and a lower flange (1021) is fixedly connected to the outer side of the top of the lower box body (102), and the upper flange (1011) is detachably connected to the lower flange (1021) by bolts; An upper sealing groove (10111) is provided at the center of the bottom surface of the upper flange (1011), a lower sealing groove (10121) is provided at the center of the upper surface of the lower flange (1021), and a sealing gasket (1013) is provided between the upper sealing groove (10111) and the lower sealing groove (10121); The upper box (101) and the lower box (102) are made of transparent acrylic sheet; The sealing gasket (1013) is made of butyl rubber.

3. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The concentration control component (200) comprises a first fixed plate (201), one end of the first fixed plate (201) is fixedly connected to the upper box body (101), one end of the first fixed plate (201) away from the upper box body (101) is detachably connected to a first three-way valve (202), one end of the first three-way valve (202) close to the upper box body (101) is connected to a first connecting pipe (2021), one end of the first connecting pipe (2021) away from the first three-way valve (202) is connected to the upper box body (101), and the first three-way valve (202) away from the first connecting pipe (2021) is connected to the upper box body (101). One end of the tube (2021) is connected to a first gas washing pipe (203), the bottom end of the first gas washing pipe (203) is connected to a first gas washing bottle (204), the top side of the first gas washing bottle (204) is connected to a second gas washing pipe (205), the end of the second gas washing pipe (205) away from the first gas washing bottle (204) is connected to a second gas washing bottle (206), the top side of the second gas washing bottle (206) is connected to a third gas washing pipe (207), and the end of the third gas washing pipe (207) away from the second gas washing bottle (206) is provided with a first vacuum pump (208).

4. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The concentration monitoring assembly (300) comprises a second fixing plate (301), one end of the second fixing plate (301) is fixedly connected to the upper box body (101), one end of the second fixing plate (301) away from the upper box body (101) is detachably connected to a second three-way valve (302), one end of the second three-way valve (302) close to the upper box body (101) is connected to a second connecting pipe (3021), and one end of the second connecting pipe (3021) away from the second three-way valve (302) is connected to the upper box body. (101), the end of the second three-way valve (302) away from the second connecting pipe (3021) is connected to the first circulation pipe (303), the end of the first circulation pipe (303) away from the second three-way valve (302) is provided with an infrared gas analyzer (304), the top end of the second three-way valve (302) is connected to the second circulation pipe (305), and the end of the second circulation pipe (305) away from the second three-way valve (302) is connected to the infrared gas analyzer (304).

5. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The humidifying assembly (400) comprises a third fixing plate (401), wherein the third fixing plate (401) is fixedly connected to the inner side of the upper box body (101), a fixing ring (4011) is fixedly connected to the bottom end of the third fixing plate (401), a humidifying tube (402) is arranged on the inner side of the fixing ring (4011), a point nozzle (4021) is arranged at one end of the humidifying tube (402), an ultrasonic humidifier (403) is arranged at one end of the humidifying tube (402) away from the point nozzle (4021), and the third fixing plate (401) is fixedly connected to the inner side of the upper box body (101), a fixing ring (4011) is fixedly connected to the bottom end of the third fixing plate (401), a humidifying tube (402) is arranged on the inner side of the fixing ring (4011), a point nozzle (4021) is arranged at one end of the humidifying tube (402), and an ultrasonic humidifier (403) is arranged at one end of the humidifying tube (402) away from the point nozzle (4021). One end of the three fixed plates (401) away from the upper box body (101) is rotatably connected to a rotating column (404), the top of the rotating column (404) is provided with an electric knob (4041), the side of the rotating column (404) is fixedly connected to a guide rail (405), the inner side of the guide rail (405) is slidably connected to an electric slide (4051), a smooth circular hole (4052) is opened at the bottom end of the book search electric slide (4051), and the humidification tube (402) passes through the electric slide (4051) along the smooth circular hole (4052).

6. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The dehumidification component (500) comprises a fourth fixing plate (501), one end of the fourth fixing plate (501) is fixedly connected to the upper box body (101), a first ventilation valve (502) is arranged below the fourth fixing plate (501), one end of the first ventilation valve (502) close to the upper box body (101) is connected to a third connecting pipe (5021), one end of the third connecting pipe (5021) away from the first ventilation valve (502) is connected to the upper box body (101), one end of the first ventilation valve (502) away from the third connecting pipe (5021) is connected to a first drying pipe (503), one end of the first drying pipe (503) away from the first ventilation valve (502) is connected to a drying ball (504), and one end of the drying ball (504) away from the first drying pipe (503) is connected to a second air pump (505); A second drying tube (506) is arranged below the first drying tube (503), one end of the second drying tube (506) is connected to the drying ball (504), a second ventilation valve (507) is arranged at the end of the second drying tube (506) away from the drying ball (504), a fifth fixing plate (508) is arranged above the second ventilation valve (507), one side of the fifth fixing plate (508) is fixedly connected to the lower box body (102), a fourth connecting tube (5071) is connected to the end of the second ventilation valve (507) away from the second drying tube (506), and the end of the fourth connecting tube (5071) away from the second ventilation valve (507) is connected to the lower box body (102).

7. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The air pressure balance assembly (600) comprises a sixth fixing plate (601), one end of the sixth fixing plate (601) is fixedly connected to the upper box body (101), a pneumatic balance valve (602) is arranged on the side of the sixth fixing plate (601), a side of the pneumatic balance valve (602) close to the upper box body (101) is connected to a vent pipe (6021), and an end of the vent pipe (6021) away from the pneumatic balance valve (602) is connected to the upper box body (101), a gas isolation frame (603) is arranged on the side of the pneumatic balancing valve (602) away from the ventilation pipe (6021), a gas supply pipe (604) is connected to the side of the gas isolation frame (603) away from the pneumatic balancing valve (602), a rotary valve (6051) is connected to the end of the gas supply pipe (604) away from the gas isolation frame (603), and a gas sampling bag (605) is arranged at the bottom end of the rotary valve (6051).

8. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The heating component (800) comprises a controller (801), wherein the controller (801) is arranged at the top center of the upper box (101), and an electric heating plate (802) is arranged below the controller (801); The heat dissipation assembly (900) comprises a sealing base (901), wherein the sealing base (901) is fixedly connected to the side wall of the lower box body (102), a heat sink (902) is arranged on the inner side of the sealing base (901), a heat dissipation base (903) is arranged on one side of the middle part of the sealing base (901), and a heat dissipation fan (904) is arranged on the side of the heat dissipation base (903) away from the sealing base (901).

9. The temperature and humidity control method according to claim 1 13 C continuous stable isotope labeling device, characterized by: The regulating component (103) comprises a rotating platform (1031), wherein the rotating platform (1031) is arranged below the tray (1041), the bottom of the tray (1041) is detachably connected to the upper surface of the rotating platform (1031), a telescopic rod (1032) is arranged below the edge of the rotating platform (1031), a lifting column (1033) is rotatably connected to the center below the rotating platform (1031), a fixing frame (1034) is arranged on the side of the lifting column (1033), and a roller is arranged on the inner side of the lifting column (1033). A ball screw (1035), the bottom side of the ball screw (1035) is rotatably connected to a support frame (1036), a first bevel gear (10351) is fixedly connected below the bottom of the ball screw (1035), a second bevel gear (10352) is meshedly connected to the side of the first bevel gear (10351), a servo motor (10353) is provided on the side of the second bevel gear (10352) away from the first bevel gear (10351), and an output end of the servo motor (10353) is fixedly connected to the second bevel gear (10352).

10. Temperature and humidity control 13 The manufacturing method of the C continuous stable isotope labeling device is characterized by: Includes the following step, (S01) preparing a transparent acrylic sheet, making the overall frames of the upper box (101) and the lower box (102), installing an upper flange (1011) on the outer side of the bottom of the upper box (101), and installing a lower flange (1021) on the outer side of the top of the lower box (102); (S02) installing a diaphragm pressure gauge (700) on the top edge of the upper box (101), installing a heating component (800) at the top center of the upper box (101), installing a regulating component (103) on the inner side of the lower box (102), and installing a heat dissipation component (900) on the side wall of the lower box (102); (S03) Passing the humidifying tube (402) through the fixing ring (4011) and the smooth circular hole (4052), installing the point nozzle (4021) on the humidifying tube (402), and then installing the humidifying assembly (400) as a whole on the side of the upper box (101); (S04) placing a sealing gasket (1013) between the upper flange (1011) and the lower flange (1021), and then connecting the upper flange (1011) and the lower flange (1021) together using bolts to form a marking chamber (100); (S05) The concentration control component (200), the concentration monitoring component (300), the dehumidification component (500) and the air pressure balance component (600) are sequentially installed on the side of the marking chamber (100), and silicone rubber is used to further reinforce the seal at all interfaces of the device. At this point, the device is completed.

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