A method and probe set for detecting EGFRvIII mutation based on DNA and RNA levels

Abstract

The present invention discloses a method and probe set for detecting EGFRvIII mutation based on DNA and RNA levels. The present invention first designs probe sets for EGFRvIII mutation at DNA and RNA levels, then constructs an intermediate library for DNA samples, and obtains an RNA sample intermediate library by fragmentation, reverse transcription and library construction for RNA samples. The DNA sample library is hybridized and captured with the DNA level probe set, and the RNA sample library is hybridized and captured with the RNA level probe set to obtain libraries enriched with target sequences, respectively. Sequencing data is obtained by high-throughput sequencing, and EGFRvIII variation information at the DNA and RNA levels can be obtained after analysis by corresponding bioinformatics analysis software. Compared with the traditional method for detecting EGFRvIII mutation, the detection method provided by the present invention is more sensitive and has higher accuracy.

Description

Technical Field

[0001] The present invention belongs to the technical field of gene detection, and specifically relates to a method and a probe set for detecting EGFRvIII mutation based on DNA and RNA levels. Background Art

[0002] The epidermal growth factor receptor (EGFR) is a transmembrane receptor tyrosine kinase composed of an extracellular ligand-binding domain (ECD), a single transmembrane domain (STMD), and an intracellular kinase domain (ICD). Abnormal expression of the EGFR gene is associated with the development of a variety of malignant tumors, including gliomas, small cell lung carcinomas, breast cancer, and bladder cancer. Glioma is a common and highly aggressive malignant tumor, with glioblastoma (GBM) being the most malignant type. However, current radiotherapy and chemotherapy are not ideal for the treatment of GBM, and the recurrence rate after surgery is high. Studies have shown that EGFRvIII mutations, also known as ΔEGFR, occur in 20% to 30% of glioblastomas. In particular, in glioblastoma samples with EGFR amplification, the proportion of EGFRvIII mutations can reach 50% to 60%. The EGFRvIII mutation refers to a deletion mutation in exons 2-7 of the EGFR gene, resulting in the loss of the extracellular domain but constitutive activation of the receptor tyrosine kinase domain, significantly promoting tumor cell proliferation, metastasis, and invasion. EGFRvIII can also further impact patient prognosis by enhancing tumor cells' ability to repair double-strand DNA breaks and their tolerance to anticancer drugs such as gefitinib and erlotinib. Therefore, the EGFRvIII mutation is an important molecular variation indicator in tumor genetic testing, particularly for glioma genetic testing.

[0003] Targeted capture second-generation sequencing technology selects target genes or regions on the genome as target detection areas for sequencing. It has the advantages of high specificity, high coverage depth and reduced sequencing costs, and is widely used in the field of tumor gene detection. The technical principles of common targeted sequencing are divided into probe hybridization capture and multiplex PCR capture. Multiplex PCR capture designs multiple pairs of primers to simultaneously amplify multiple target sequences in a single reaction. Its advantages are low starting amount requirements and simple and fast detection process. However, the design of multiplex PCR primers is difficult, and subsequent supplementation and updating of sites is difficult; the coverage of the detection area is small; and there may be amplification bias during PCR amplification, affecting the detection sensitivity. The probe hybridization capture method can simultaneously capture thousands of different target regions in a larger range. The probe design is less difficult, the target update is highly flexible, and the detection sensitivity is higher.

[0004] The breakpoints of large deletion fragments at the DNA level of EGFRvIII mutations generally occur in intron 1 and intron 7, and the positions are not fixed. In particular, the sequence of intron 1 is about 123kbp, which is long and complex. Therefore, it is difficult to detect EGFRvIII mutations at the DNA level based on multiplex PCR capture sequencing technology due to the difficulty in primer design and small coverage. In contrast, the detection of sequences in the EGFR structural variation region can be specifically enriched by probe hybridization capture sequencing technology. The probe design is easy and the coverage is large, which can realize the detection of EGFRvIII mutations. However, the number of probes required for DNA-level detection is large, and the amount of sequencing data required is high, resulting in high detection costs.

[0005] The transcripts of EGFRvIII mutation at the RNA level are relatively fixed as the fusion of exon 1 and exon 8. It is easy to design primers or probes for targeted capture sequencing of EGFRvIII mutation at the RNA level, and the detection positive rate is high. Studies have shown that multiplex capture sequencing failed to identify the deletion of exons 2-7 of the EGFR gene at the DNA level, but the fusion of EGFRvIII exon 1 and exon 8 could be detected at the RNA level. However, the coverage of the target area of ​​multiplex PCR capture is small, and it is only suitable for the detection of fusions with known breakpoints. It cannot capture fusions with breakpoint positions outside the coverage range and unknown fusions; it is also easily affected by RNA quality, and has poor fusion detection ability for low-quality RNA samples with severe degradation. Relatively speaking, the target coverage of probe hybridization capture technology is large, and it can capture fusions with unstable breakpoint positions and unknown fusions, and the impact of RNA quality on detection ability is small. Summary of the Invention

[0006] In order to make up for the deficiencies of the prior art, the purpose of the present invention is to establish a method for detecting EGFRvIII mutations at the DNA level and the RNA level based on probe-targeted capture technology.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a probe set for detecting EGFRvIII mutation at the DNA level.

[0009] Furthermore, the probe group comprises any one of the probes having nucleotide sequences as shown in SEQ ID NOs. 1 to 1032, or probes having a homology of more than 70% with the sequences shown in SEQ ID NOs. 1 to 1032.

[0010] A second aspect of the present invention provides a probe set for detecting EGFRvIII mutations at the RNA level.

[0011] Furthermore, the probe group comprises any one of the probes having a nucleotide sequence as shown in SEQ ID NOs. 1033 to 1037, or a probe having a homology of more than 70% with the sequence shown in SEQ ID NOs. 1033 to 1037.

[0012] In some embodiments, probes corresponding to sequences having more than 70% homology with the probe sequences described in the present invention are also included in the protection scope of the present invention, that is, it can be understood that probes obtained after modifications based on the probes provided by the present invention also fall within the protection scope of the present invention.

[0013] A second aspect of the present invention provides a kit for detecting EGFRvIII mutation.

[0014] Furthermore, the kit comprises the probe set described in the first aspect of the present invention and / or the second aspect of the present invention.

[0015] Furthermore, the kit also contains any one or a combination of at least two of a DNA fragmentation reagent, an RNA fragmentation reagent, a cDNA single-strand synthesis reagent, a cDNA double-strand synthesis primer, a cDNA double-strand synthesis reagent, a linker connection reagent, a hybridization capture reagent, a hybridization product amplification reagent, or streptavidin affinity magnetic beads.

[0016] In certain embodiments, the kit may contain reaction reagents necessary for performing sequencing, including but not limited to DNA fragmentation reagents, RNA fragmentation reagents, cDNA single-strand synthesis reagents, cDNA second-strand synthesis primers, cDNA second-strand synthesis reagents, adapter ligation reagents, hybridization capture reagents, hybridization product amplification reagents or streptavidin affinity magnetic beads.

[0017] In the present invention, the kit further comprises instructions, which may include guidance on obtaining samples and processing samples.

[0018] A fourth aspect of the present invention provides a method for preparing a library for detecting EGFRvIII mutations.

[0019] Furthermore, the method includes performing a mixing reaction on a DNA and / or RNA intermediate library obtained from the sample to be tested using the probe set described in the first aspect or the second aspect of the present invention to obtain a library for detecting EGFRvIII mutations.

[0020] Furthermore, the DNA and / or RNA intermediate library comprises DNA and / or RNA molecules that have undergone fragmentation, end repair, end "A" reaction, adapter ligation, and PCR amplification in sequence.

[0021] Furthermore, the sample to be tested includes tumor cells, blood, plasma, exosome solution, pleural effusion, peritoneal effusion, saliva, urine or tissue.

[0022] A fifth aspect of the present invention provides a method for detecting EGFRvIII mutation at the DNA level.

[0023] Furthermore, the method includes performing probe hybridization capture on a DNA intermediate library obtained from the sample to be tested using the probe set described in the first aspect of the present invention.

[0024] Furthermore, the step of obtaining a DNA intermediate library from the sample to be tested includes extracting DNA from the sample to be tested, fragmenting the sample DNA, performing end-repair, end-addition "A" reaction, linker connection, PCR amplification and purification on the fragmented DNA, and then using Qubit quantification and 2100 quality control detection to obtain the DNA intermediate library.

[0025] Furthermore, the method also includes the steps of library amplification and purification after probe hybridization capture, sequencing and analysis of sequencing results.

[0026] Furthermore, the sequencing includes performing sequencing through a sequencing platform to obtain original offline data of the sample to be tested.

[0027] Preferably, the sequencing is performed using the Illumina platform in PE150 mode.

[0028] Furthermore, the step of analyzing the sequencing results includes comparing the original offline data of the sample to be tested with the hg19 version of the human reference genome to obtain the large-fragment variation results of the EGFR gene. If the breakpoints of the large-fragment variation belong to intron 1 and exon 7 to intron 7, respectively, and no less than 4 sequencing reads support the variation reads number, it is judged to be EGFRvIII positive.

[0029] Furthermore, the sample to be tested includes tumor cells, blood, plasma, exosome solution, pleural effusion, peritoneal effusion, saliva, urine or tissue.

[0030] A sixth aspect of the present invention provides a method for detecting EGFRvIII mutation at the RNA level.

[0031] Furthermore, the method includes performing probe hybridization capture on an RNA intermediate library obtained from a sample to be tested using the probe set described in the second aspect of the present invention.

[0032] Furthermore, the step of obtaining an RNA intermediate library from the sample to be tested includes extracting RNA from the sample, fragmenting the sample RNA, and sequentially subjecting the fragmented RNA to cDNA single-strand synthesis, cDNA second-strand synthesis, end repair, end "A" reaction, and adapter ligation, PCR amplification and purification, and then using Qubit quantification and 2100 quality control detection to obtain the RNA intermediate library.

[0033] Furthermore, the method also includes the steps of library amplification and purification after probe hybridization capture, sequencing and analysis of sequencing results.

[0034] Preferably, the sequencing is performed using the Illumina platform in PE150 mode.

[0035] Furthermore, the sequencing result analysis step includes aligning the captured RNA molecule sequencing data to the hg19 version of the human reference genome to obtain alignment information for each sequencing read; merging the alignment positions of the two reads R1 and R2 obtained by sequencing the same RNA molecule fragment from both ends into a complete read; and classifying according to the alignment information of the merged reads:

[0036] 1) If a read segment is soft-truncated, the front end of the read segment can be correctly aligned to exon 1 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 8. The read segment is recorded as m and the breakpoint is recorded as M.

[0037] 2) If a read segment is soft-truncated, the front end of the read segment can be correctly aligned to exon 8 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 1. The read segment is recorded as n and the breakpoint is recorded as N;

[0038] 3) The total number of sequencing reads is recorded as s;

[0039] The reads classified as 1) and 2) are the reads supporting the EGFRvIII mutation. The number of supporting reads is then normalized to the number of variant supporting reads per megabyte of sequencing reads, calculated as follows:

[0040] Number of variant support reads per megabyte of sequencing reads = ((m+n)*1000000) / s;

[0041] If the number of variant support reads per megabyte of sequencing reads is greater than or equal to 0.2, the sample is judged as positive, otherwise it is judged as negative.

[0042] Furthermore, the sample to be tested includes tumor cells, blood, plasma, exosome solution, pleural effusion, peritoneal effusion, saliva, urine or tissue.

[0043] In the present invention, the tumor cells include brain glioma, pharyngeal cancer, adrenal tumor, AIDS-related cancer, alveolar soft tissue sarcoma, astrocytoma, bladder cancer, bone cancer, brain and spinal cord cancer, metastatic brain tumor, breast cancer, carotid body tumor, cervical cancer, chondrosarcoma, chordoma, renal chromophobe cell carcinoma, clear cell carcinoma, colon cancer, colorectal cancer, desmoplastic small round cell tumor, ependymoma, Ewing tumor, extraskeletal myxoid chondrosarcoma, fibrous dysplasia, fibrous dysplasia, gallbladder or bile duct cancer, gastric cancer, primary liver cancer, malignant teratocarcinoma, ovarian embryonal tumor, viral hepatitis, gestational trophoblastic disease of liver cirrhosis, germ cell tumor, head and neck cancer, liver cell Preferably, the tumor cell is a glioblastoma cell.

[0044] Advantages and benefits of the present invention:

[0045] 1. This invention provides methods for detecting EGFRvIII at both DNA and RNA levels. Depending on the availability of nucleic acid samples, DNA and RNA co-detection, DNA alone, or RNA alone can be flexibly selected. Compared to DNA alone, RNA-based detection is unaffected by introns and can improve the detection rate of EGFRvIII. Co-detection of DNA and RNA is recommended when conditions permit.

[0046] 2. Compared with the method of detecting EGFRvIII using multiplex PCR capture technology, the detection method developed in the present invention based on the probe targeted capture principle is less affected by low-quality RNA, can capture fusions of other patterns, and can also be flexibly added to other probe pools of the same type to complete multi-target simultaneous detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 This is a schematic diagram of the design of the EGFRvIII mutation detection probe provided by the present invention. DETAILED DESCRIPTION

[0048] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased on the market.

[0049] The present invention aims to establish a method for detecting EGFRvIII mutations at the DNA level and RNA level based on probe-targeted capture technology. The present invention first designs a probe group for detecting EGFRvIII mutations at the DNA level and RNA level. The DNA level probe group covers exon 1, intron 1, exon 7, intron 7 and exon 8 of the EGFR gene with 1× probe coverage, which can capture large fragment deletions from intron 1 to intron 7 of the EGFR gene at the DNA level. The RNA level probe group is designed to cover exon 1 and exon 8 of the EGFR gene with 1× probe coverage, and is designed to include a cross-breakpoint fusion probe sequence containing a 60bp sequence near the 3' end of exon 1 and a 60bp sequence at the 5' end of exon 8, which can capture the fusion of exon 1 and exon 8 of the EGFR gene at the RNA level. Then, an intermediate library is constructed from the DNA sample, and the RNA sample is fragmented, reverse transcribed and library constructed to obtain the RNA sample intermediate library. DNA sample libraries are hybridized and captured with DNA-level probe sets, while RNA sample libraries are hybridized and captured with RNA-level probe sets to generate target sequence-enriched libraries. Sequencing data is generated through high-throughput sequencing, and then analyzed with appropriate bioinformatics analysis software to obtain EGFRvIII mutation information at the DNA and RNA levels.

[0050] Example 1 DNA and RNA detection of known EGFRvIII positive clinical samples

[0051] 1. Step 1 DNA library construction and probe hybridization capture

[0052] 1. Experimental Materials

[0053] Samples: DNA from 6 clinical samples with known EGFRvIII mutation positive RNA level;

[0054] Library construction reagents: A commercial DNA library construction kit specifically developed for the Illumina high-throughput sequencing platform; Dual-end UDI adapters: Commercial 8nt dual-end unique index adapters compatible with the Illumina high-throughput sequencing platform;

[0055] Hybrid capture reagents: Commercially available Sequencing platform DNA library hybridization capture kit;

[0056] Hybridization capture probe: e.g. Figure 1 The DNA level probe pool designed and synthesized for detecting EGFRvIII mutation at the DNA level has a total of 1032 probes designed at the DNA level, and the sequence information of the DNA level probes is shown in SEQ ID NO.1-1032.

[0057] The DNA level probe sequence information is as follows:

[0058] EGFRvIIIDprobe0001:TCTTCGGGGAGCAGCGATGCGACCCTCCGGGACGGCCGGGGCAGCGCTCCTGGCGCTGC TGGCTGCGCTCTGCCCGGCGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTcg(SEQID NO.1); EGFRvIIIDprobe0002: GTAAGGGCGTGTCTcgccggctcccgcgccgcccccggatcgcgccccggaccccgcagcccgcccaaccgcgcaccggcgc accggctcggcgcccgcgccccggcccgTCCTTTCCTG (SEQ ID NO.2);

[0059] EGFRvIIIDprobe0003:TTTCCTTGAGATCAGCTGCGCCGCCGACCGGGACCGCGGGAGGAACGGGACGTTTCGTT CTTCGGCCGGGAGAGTCTGGGGCGGGCGGAGGAGGAGACGCGTGGGACACCGGGCTGCAGG(SEQID NO.3);EGFRvIIIDprobe0004:CCAGGCGGGGAACGGCCGCCGGGACCTCCGGCGCCCCGAACCGCTCCCAACTTTCTTCC CTCACTTTCCCCGCCCAGCTGCGCAGGATCGGCGTCAGTGGGCGAAAGCCGGGTGCTGGTG(SEQID NO.4)EGFRvIIIDprobe0005:GGCGCCTGGGGCCGGGGTCCCGCACGTGCGCCCCGCGCTGTCTTCCCAGGGCGCGACGG GGTCCTGGCGCGCACCCGAGGGGCGGGCGCTGCCCACCCGCCGAGACTGCACTGTTTAGGG(SEQID NO.5)EGFRvIIIDprobe0006:AAGCTGAGGAAGGAACCCAAAAATACAGCCTCCCCTCGGACCCCGCGGGACAGGCGGC TTTCTGAGAGGACCTCCCCGCCTCCGCCCTCCGCGCAGGTCTCAAACTGAAGCCGGCGCCCG(SEQID NO.6)EGFRvIIIDprobe0007:CCAGCCTGGCCCCGGCCCCTCTCCAGGTCCCCGCGATCCTCGTTCCCCAGTGTGGAGTCG CAGCCTCGACCTGGGAGCTGGGAGAACTCGTCTACCACCACCTGCGGCTCCCGGGGAGGG(SEQID NO.7)EGFRvIIIDprobe0008:GTGGTGCTGGCGGCGGTTAGTTTCCTCGTTGGCAAAAGGCAGGTGGGGTCCGACCCGCC CCTTGGGCGCAGACCCCGGCCGCTCGCCTCGCCCGGTGCGCCCTCGTCTTGCCTATCCAAG(SEQID NO.8)EGFRvIIIDprobe0009:AGTGCCCCCCACCTCCCGGGGACCCCAGCTCCCTCCTGGGCGCCCGCGCCGAAAGCCCC AGGCTCTCCTTCGATGGCCGCCTCGCGGAGACGTCCGGGTCTGCTCCACCTGCAGCCCTTC(SEQID NO.9)EGFRvIIIDprobe0010:GGTCGCGCCTGGGCTTCGCGGTGGAGCGGGACGCGGCTGTCCGGCCACTGCAGGGGGG GATCGCGGGACTCTTGAGCGGAAGCCCCGGAAGCAGAGCTCATCCTGGCCAACACCATGGTG(SEQID NO.10)EGFRvIIIDprobe0011:TTTCAAAATGGGGCTCACAGCAAACTTCTCCTCAAAACCCGGAGACTTTCTTTCTTGGAT GTCTCTTTTTGCTGTTTGAAGAATTTGAGCCAACCAAAATATTAAACCTGTCTTacacac(SEQID NO.11).

[0060] EGFRvIIIDprobe0012:cacacacacacacacacacacacCGGATTGCTGTCCCTGGTTCAAGTGTGCCAAGTGTGCAGACAGA ACATGAGCGAGTCTGGCTTCGTGACTACCGACCATAAACCCACTTGACAGGGG(SEQID NO.12)

[0061] EGFRvIIIDprobe0013:AGGGGAAACATGCCTTGGAAGGTTTAATTGCACAATTCCAACCTTGAGCTGCGCGGGTT CCAAGAGCCAGGCCCGTACTTGCTGTTGATGTCATTGGCTTGGGGAGTTGGGGTTTGGTGC(SEQID NO.13)EGFRvIIIDprobe0014:CCAGCGCGGTCGTTGGGGGAGGGGCAAGGCATAGAAcagtggttcccagaccttgctgcacattggaattacct gggattaaaaaaaaaaaaatcaaaacaaaaaccagtgtctggctcc(SEQID NO.14)

[0062] EGFRvIIIDprobe0015:cgcccccagacattctgatttaattggcatggggcaagacctggacttgggattttttttaatgctcttcatgtgatctgttgggcagccagatttgggg atcactAGACGGAAGAAGGA(SEQID NO.15)

[0063] EGFRvIIIDprobe0016:TTGTTAAAGTCTCCGGAGATGTTACTTGCCAATGCTAAGAGCTCTTTGAGGACATCTGGA ATTGTTACAATATTGCCAAATATAGGAAAGAGGGAAAAGGTAGAGTGTGATTCCAATAAT(SEQID NO.16)EGFRvIIIDprobe0017:AAAGGATTCCGCTTTTCATTGAAGGAACTGGTGGAAAGGTTTCTTCTCTGCTGAGCCTGC AGGCCCGTCCTGCCTGCCTGGGGTGCCCGGGAGACGCGGGCCTGCTCCGGAGACTGCTGA(SEQID NO.17)EGFRvIIIDprobe0018:CTGCCGGTCCTGTTAGTCAGGTGTCAGCCCTGTCTCTGCCGAAGAGACTCTTCTCTTTATT TTAAATTAAACCCTCAGAGCACCACCAAAGCATCACTTTTCTCCCTCCATTGGTGTTCT(SEQID NO.18)EGFRvIIIDprobe0019:CATTCTTTGATGTTACTTGTTTGAACACCACTATTAGTAGTTGGAGATTTGTTCCTGAGAA AAATATAAATACCACTTAATTTGCCTGTTTGTCCCGCATTCACTCAAAACAGAATGCTC(SEQID NO.19)EGFRvIIIDprobe0020:CTGAAGACAAGAGAGAGAGTAGGAGAACAGACGCTATTCCATTACAGTAACATAAAAGA CTGGATTTTCAGGGGCAAATTATTAAAATAGGAGATGAGCTCTTTTAACAGAAATTTGTTT(SEQID NO.20)EGFRvIIIDprobe0021:AAGGCCTGTGTCTATCAAATTCAGTGGATTTTATTCAAGATGCACTTTGTTTAGTGGGAGT

[0064] TTTGTTTGGTTCTGGGACATGCTAACTTCTAGACTTGCTGCTCTTAGAGGTAATGACTG(SEQ IDNO.21)

[0065] EGFRvIIIDprobe0022:CCAGACACCATTTCATGAGTCCtaatccccacattaagcataagaggtgcacactctcctcctatgggggaaactgaggtacg

[0066] aagaactaaagtgactttcccacagctggtgggaggc(SEQ ID NO.22)

[0067] EGFRvIIIDprobe0023:agacgggaaattcacaccaggggcttccaactccagatccctctctcaacttccaaactccactgccttgTCCGAGTTCTGGTTTCA

[0068] GGAGATCCAAATCAGGTGTGTGCAAATGTCTAA(SEQ ID NO.23)

[0069] EGFRvIIIDprobe0024:TGTCAGAGCTGGCAAGGGGAAAGGGCCCAGGGAGCCGGCTCATGACGATGAGCCTGTC

[0070] TGAAGCTTCAACGCGGGCTGTCCGGCAGTCTGCATTCCTGCCGAGTTCCTCAGCCCTCTGTT(SEQ IDNO.24)

[0071] EGFRvIIIDprobe0025:GGGTCACCTTCCATAGAGGCAGCTTAGTCCTCAGTTCAGTGAGCATGGAGTGGAGACTG

[0072] CTTGAGGGGTGCTGAGCAAAGCCCTGCCTCTTACAGGATGAAGGTGCTCTCCAGAAGGGAC(SEQ IDNO.25)

[0073] EGFRvIIIDprobe0026:ACTGGAAAGTATTCCAAGGCGAGTCGAATTCCCAACTGAGGGAGCTTTGTGGAAATAAG

[0074] CCCGCCCAGCCCCACTTCTGGAGACGTTCCCATTCAGTAGGTCCGAGCTGTCTTAAAGAGA(SEQ IDNO.26)

[0075] EGFRvIIIDprobe0027:AACCAAAGTGGGGATATTAATGGTATCCAAAGTGAGATCTACCCCACCCTCCCTCCTCAA

[0076] AGGAGGTCAGATCAAGAAAGCCCAAGCCCGGCCTGGCAATTGGGACCTTTCTTCTCACTC(SEQ IDNO.27)

[0077] EGFRvIIIDprobe0028:CAGCCCAGGGTGAAGGTGGACAAGTCACTTTGACCCTTCAGGCTTCTGAGCTGTTGTTT

[0078] CTGAATTCAGTGAATATTTACTGAGTGCATAGAATATGCTAGATATTCTGGGCTAAAGGTT(SEQ IDNO.28)

[0079] EGFRvIIIDprobe0029:GAAGGGGGGGTGAGTTTTAAGGGTTTCTGCTCTTGCTTCCAGATTGCTTTCAAATCTGGA

[0080] AAGGACACCAGTGGTTTGTGTGTTAGACCCACACTGCCGTAGCACAGAATACAAGAAACT(SEQ IDNO.29)

[0081] EGFRvIIIDprobe0030:GGCTGAGAGCTCCAATAGGCTTTTAACAGTAATTTCTGGCTTCACGTATTTAGTTTCATAA

[0082] CTCATGATTTTTCAAAAACTTCTGGTTTGAAGACACCGATTGCCGAAAGTCCATTGTGC(SEQ IDNO.30)

[0083] EGFRvIIIDprobe0031:TGCATAATTACACTTGGTCCACGTGACAGCACTAACATGTTCTGAAATGTTTTTAGAAGTA

[0084] GTCTCAGCAAAGATGAAGGATTCCTCCCTGTTTGAAAAGAAAATATTCTTTGTTTTTTC(SEQ IDNO.31)

[0085] EGFRvIIIDprobe0032:TTTGATCTAAGCTCTAAGACTAGCAGCTAGCATCTGAAACTTTTTTGACGAGAGTGACAA

[0086] ACCAACTCTAATATTAAAGGCAATTGATGATTATGGGCACTGAAGGGAAGGTAACCCCAG(SEQ IDNO.32)

[0087] EGFRvIIIDprobe0033:GCTGGTGCCCCGGAATAGGGATGGGTCACAATGTTGAGGACATTTCGCCTGTTGCAGAA

[0088] CCCACCTGCAACACAGTGTGGCCCTTGCCATGTGACTtgtgtgtgtgcctgtgtgtctgtg(SEQ IDNO.33)

[0089] EGFRvIIIDprobe0034:tgtgcgtgtTTTAATTTTGACTTCATAAGTACTCTAGTTATGAGCTTATTTAACATTGGGTTTTAC

[0090] TAATAGGGGTATGTGTTGAGAAAATTTCAAAGTTTTAGAATATGGTTCACCCAC(SEQ ID NO.34)

[0091] EGFRvIIIDprobe0035:ATGTTGCTTCCCTGTAAATATAATTTTTAAAACCAGATTCTGggccgggcatggtggctcacctctataatcc

[0092] caaaacgttgggaggccgaggcaggcgaatcatgaagccaggagttt(SEQ ID NO.35)

[0093] EGFRvIIIDprobe0036:TAATTTTTAAAACCAGATTCTGggccgggcatggtggctcacctctataatcccaaaacgttgggaggccgaggcaggcga

[0094] atcatgaagccaggagtttgagaccaggctgaccaacac(SEQ ID NO.36)

[0095] EGFRvIIIDprobe0037:atctcaaaaaaaaaaaaaaaaaaaCAGATTCTGTTCCTCAGATCCATTCCATTTTTGTTTTCCTTTATCAC

[0096] TTATGGACATTTGAAATTATGGTAATAAACATTGTTAGTCTCAGTTAAT(SEQ ID NO.37)

[0097] EGFRvIIIDprobe0038:GATCCATTCCATTTTTGTTTTCCTTTATCACTTATGGACATTTGAAATTATGGTAATAAACAT

[0098] TGTTAGTCTCAGTTAATTATTACTGGTTTATTCTTGAACCACTAATCCATAGAGAAT(SEQ IDNO.38)

[0099] EGFRvIIIDprobe0039:AGAGTGTAAATCTTAACTTGTTCCTGTAGGCCATCCCCATTAAACATCATAGTGTTTTCTCA

[0100] TTCGTTCTTTTTCGTTTTCCTCCTACAGGAATGAATTTTCTAAGAAAATTCCAGCAGT(SEQ IDNO.39)

[0101] EGFRvIIIDprobe0040:TGGCTCTTTGGACGACATCTCTAGATTGTCCTCCATTGGGCCCATAGGCACAAGCTGGCC

[0102] AGTTTGAATTTGGGCAAGAATCCAGGCATTGGAACTTATTCAAATAACTAGTTTGCCTGT(SEQ IDNO.40)

[0103] EGFRvIIIDprobe0041:AATTTTCACTTTTTCAGAGTCATCTGATAAAGCTTTCTTGCTACACATTTAGATAGATACAC

[0104] TCAATCCAGTTGTCTAGAAAGTTCCCTGAGCCAGCTGGGAGCAGGAGGGGTAGTTGGG(SEQ IDNO.41)

[0105] EGFRvIIIDprobe0042:GCCAGGAATATTGGGGGTGTGTTTACTGAGCCCCTAGAAAGTAAGTGCTAGATTTGACAT

[0106] TTCAATCCCTGAAGGCCCTGAAGTTCAGTATCAAATGACTGGTCCTGTGGACTGAGCATC(SEQ IDNO.42)

[0107] EGFRvIIIDprobe0043:TGTGAATTGCATATGCTTAGAGTAAATTTTACTCCTACCAGTTTCAGCAGCTTGCTTTAGC

[0108] AAGCAGTATGGAAACACTAACATGGGGGAGTAGAATTTCTCTCTCTGATCCAAGTTTTA(SEQ IDNO.43)

[0109] EGFRvIIIDprobe0044:TCTCATTCTGGTGGGTTTTCAAGGAGAGACTCGGAGTCCAAGTGTCCTTTCTGAATATATC

[0110] TGGAACTTCTCATTAACAAAAGACTCAAGTTATAATTTAGGGGACAAGGCACCCAATGA(SEQ IDNO.44)

[0111] EGFRvIIIDprobe0045:GAATGCCTTGCAGGCAGCCCTAAGTACACCTGCAATTACACCATTACTAGCGCGGCAGCA

[0112] CACATGGCCCTGACTTAGTTTAAATAATTACGTAAGTCAACCATGATTGTTTGCCCTTTG(SEQ IDNO.45)

[0113] EGFRvIIIDprobe0046:CATAGAAGGGCAAGTATTGGTACCTGTTACAACTTAGGCTTTTTTTTCTTTATGTTTGAGC

[0114] CATGATGAGTGATTTACACTGTTGCATCCATATGTTGAGATGTAAGAATAAATTAGACT(SEQ IDNO.46)

[0115] EGFRvIIIDprobe0047:TGGTAATTGCCCTTAAGTGTCTGGAAGTCAACTGGGGAAAGAGAGCTAGAGATAATAAG

[0116] TGTGAAACAATGTCACAGAATCAATGACGGAACTCTTCCCAGGACAAAGGATGACTTTTGA(SEQ IDNO.47)

[0117] EGFRvIIIDprobe0048:GTTCAGTCTTTGCCTTTAATTCTACATGGGGAGGAGAGCACGTTTAGCCACAAATGGAAG

[0118] GGATTACTCATTTGAGCTATTTGGTTATATGATTATTTCCCCAGAGAATAGGATGTGCAG(SEQ IDNO.48)

[0119] EGFRvIIIDprobe0049:GGCATTACACAAGCAGTGCCAATAGCAGCAAAGTTCTTGAGAGTGCTAGTAATTCAAATG

[0120] GCAGGAAGAGAAGGAATAAATGGTAAGGCTACCTACAGTTCACAGAGAGCTCCATCCTca(SEQ IDNO.49)

[0121] EGFRvIIIDprobe0050:ctgtggctttggattttgtcctgtgtgaaagagaagtgactgtgaactgacatgctgtgtttggtgttttagaaagatggctgcagcagcggtttgggg

[0122] aatggactgcaggagtggcat(SEQ ID NO.50)

[0123] EGFRvIIIDprobe0051:tggaaacaggaaggttcatgactattgccagagacagaggatgaagcaggagcaaggaagattcaggacaggggactccggggctgatcag

[0124] gaggcagaactggttgataagtatatgta(SEQ ID NO.51)

[0125] EGFRvIIIDprobe0052:gcagcataagaaagaaagaatcccagattgacacccaggcttctcacttggaagcctggatagatactgaatgcaatcacaaaggctgggaagt

[0126] caatgggactgcagggaagggaaggg(SEQ ID NO.52)

[0127] EGFRvIIIDprobe0053:aagggaggagaagaggaagggcaggagggtccaatatcaatattcagcttttagatgtgttgagcttgaagtgctcagatggagaagtccagga

[0128] ggcagtagaatacggtggtccagagc(SEQ ID NO.53)

[0129] EGFRvIIIDprobe0054:acaggagagcaatgtggcttgagTTGTCATTTGCTCACATATTTCCGTGTCAGTTACTTGTCTTAGATCA

[0130] CAGAACAAGTTCTCCTCTCACAGTTTCCTGGCTCCACCTGTCTCATGCTC(SEQ ID NO.54)

[0131] EGFRvIIIDprobe0055:ACCGTCAGCATCGAAATTGAGCCACACCAGGGGTTCTGGATACCAGCTTCTCTCTAGGTG

[0132] AGGCTGCTATAGTCAGCAGCTGATTAGTTGCAGTTATCAGCAACTGGTAATATAATATAT(SEQ IDNO.55)

[0133] EGFRvIIIDprobe0056:TGTGCATATAAGTGTACCAGAAGTCATGTTTATATATTGCTGCAAATACTCGGAATGGGGA

[0134] TCTCTTGTTCCCTGCTTAAGACCACATCACATTACTTGGTTTTGTACGCTAGTGGCTGA(SEQ IDNO.56)

[0135] EGFRvIIIDprobe0057:ACCAAAAAAAGTAGGAGATGATTTTTTTTCTTTTTTCTTAAAGcagtagcttttgaaccttgaccatgctttct

[0136] aaccagctgaggggcttttgaaaaagagggtgccttactgtgcccc(SEQ ID NO.57)

[0137] EGFRvIIIDprobe0058:agaccaggacaatcagtatttctggggaatggagcctggcacacacacatttcttaaagctcccttggcaattcTGAGGAGTGGATTA

[0138] CATGTTGTATGTAGCTCGTAACGAAAGAAATC(SEQ ID NO.58)

[0139] EGFRvIIIDprobe0059:TTGTCTTTGCTCTCAGACCCCCATTTCTTACTCATCTCATGAGCTCCTTCGAGATCCAGAA

[0140] ACAGTTGCATATTTCATTAGTAAATCAGTTCCAGAGTCACATTTTATTTCACAAGTTAG(SEQ IDNO.59)

[0141] EGFRvIIIDprobe0060:GTTAGTCCATTAAAAGTTTCCTGCAGTGaggaaatagccagaaagaacactccacccctcctcctttttataactatagg

[0142] gtctggCTCgacagagcaggagcatcgccatcttggacaa(SEQ ID NO.60)

[0143] EGFRvIIIDprobe0061:ctcctcgaccagagacatgctagtcccgagataacccccctccagcagggaagatgccagtctcgggataacctctctctggccggaaagatgc

[0144] ctgccccaagataaacttgcctcctc(SEQ ID NO.61)

[0145] EGFRvIIIDprobe0062:ccagagatattccaaccctgccataaaacttctccctcaaacaggaacattccaaaattctgataatctccctcaccctaaaaccaatatatactccta

[0146] gtctgtaagagaaagcgctct(SEQ ID NO.62)

[0147] EGFRvIIIDprobe0063:tgaccaaaattcaccaggagtgcctcccaggttttaactaaagaaaacctctctttaactgCCAAAAAAAAAAAGGGAAAAA

[0148] AAAAAGCTTTCTGcagtggctttcagcgggcccagcat(SEQ ID NO.63)

[0149] EGFRvIIIDprobe0064:ggcagcagcacctgagaacctgttggagatgcacactcttggaccccaccctggcctctgagtaagacactggaagggcaggccccggtctgt

[0150] gcacacaagtcctcagggagattctga(SEQ ID NO.64)

[0151] EGFRvIIIDprobe0065:ctgatgcatgccagattttgagaactgctgATATACTCCAGGCACATCGCATGCTGGGATCTAGATACACCA

[0152] AGGGAACAAAATAACTGCACTTGTCCTCTGAGGACCGACTTACCTTTT(SEQ ID NO.65)

[0153] EGFRvIIIDprobe0066:GGAAGGGCTGAGAAAGAGAGACACATACAAGATCACTCCCTGTAATGCAATGTTTTATAA

[0154] CAGATGTGATTTGGGATTTCAGTGGGAGCCCAAAAGAGGGACTGACTAATTCAGCCTCTG(SEQ IDNO.66)

[0155] EGFRvIIIDprobe0067:TGACAAGGGGAGTTTCTCAGAAACAGAATGCTTAGCTGGGCCTCCAGGCACAGGGACA

[0156] GGAATGAGGAAATACTTGTAGGCCCTGTGCTCCTTCAGCAAAACCCTCAGTTTCTTGTTATT(SEQ IDNO.67)

[0157] EGFRvIIIDprobe0068:TTTATAAATGCAAACATCTTATTAAAGTAGATGCTAAGGCATTAGAATTTCCTGCTTTATTT

[0158] TTCTAAATGACCATGAGGAAACCTGGAATGTCAAAGATAAAGTGCAACACATTCTGCA(SEQ IDNO.68)

[0159] EGFRvIIIDprobe0069:TTTAAAAATTAAAATGATCCTTTTTAAAAGTAGCAACCAGATGTGAAAAATTGGACTGGA

[0160] GTCCAGGTTATAGTTGATAGCTTTAACTTTCTCCCCAACAGCAACAGCACAATTTTCCCT(SEQ IDNO.69)

[0161] EGFRvIIIDprobe0070:AAAATGTGTTATGAATAAGTAAAATGACTACTTCACATCCTTTAACTCTTCCTACAGAAAT

[0162] CTAAGAGAGAAATGAAACAAAAGTTTGCACAGTTCTAGACACGATAAATACATGTGAAA(SEQ IDNO.70)

[0163] EGFRvIIIDprobe0071:TCACACAACTCAGAAAATGTCCCTTAAATTAATTGAGCCATTGGTACTTGTGAATTAGAA

[0164] GAGACATCTATGTTCTGATCCACTGTTGAAAGCTGTACAATGTTACCTATTTATTTGCAG(SEQ IDNO.71)

[0165] EGFRvIIIDprobe0072:ACATCCTTTGGAAACAAATAGGTAGATTTGCAACAAATAAAGAGTGGAGTACAGCTGCT

[0166] GACATTACCTTGTATATTCATGCCTTTATGTAAAAAAAAAAAAAAAAatatatatatatat(SEQ IDNO.72)

[0167] EGFRvIIIDprobe0073:atatatatatacacacacacacacatatGGAGGTAAAGACCACTGCTTGCTTTGCAGTTGTTTTAAGAGCA

[0168] TTCATGAAGGATTTTATTTTATAAGCAGAAATGTGATATCTGACGATTT(SEQ ID NO.73)

[0169] EGFRvIIIDprobe0074:CTGACGATTTTACCACTACATGCTTGCAGGCCAGTGCACAGCAGATGACGTCATGATTGT

[0170] TTTAGCAGTCCTATCGTTTTACTTATGATGTCATTACAACCCTTTGCTAAAATTTCTTTC(SEQ IDNO.74)

[0171] EGFRvIIIDprobe0075:CTTTACTCCAGGTTTTGGATAAAATTGATGCATTGCACATAGTCTCTCTGATAAGACAAAC

[0172] TGGCATTTGTATGTGAAAAACTGTGCATGTTTTAGTGTCTCTGCTGATACTCAAATTAT(SEQ IDNO.75)

[0173] EGFRvIIIDprobe0076:CCATTATTTTAGTGCTGGAATAAAAACAAACCACTTAGTGAATTTGTGCAGGTCCTTAAG

[0174] GACAGGCAAAGGTGTCCTGAGATTTTCTGATCATTGTATACCAAATTTTAGAAACTTTTT(SEQ IDNO.76)

[0175] EGFRvIIIDprobe0077:CAAAAACATTTTTTTAATTTCAAAAACCTGGTTTTGTTTATTTACCAGCAATCATTGAATAC

[0176] CTGAAAGCTTTCAGGAGATTTTATTACAATGGTTTCTATTCACTTACAAAATTATCTC(SEQ IDNO.77)

[0177] EGFRvIIIDprobe0078:CTAGTTCATTCTCATACACTGTAAGCCATTGTAAATGCTTCAAATTGTGCCGAACAAGATA

[0178] AACTAGACAAACTATTTTAAGTTTGTTCTAGTGCTAACTTGCAAGATCTAATGGCTCCA(SEQ IDNO.78)

[0179] EGFRvIIIDprobe0079:ACTAGatttttaaaataaagtatattttaatatattattaGAAAGTTAAGCAATTATCTGTTTATAGGTAACAAAA

[0180] ACCCTGGAACCCCAATGTCAGATGTCATCCACTTTTGATTAAGT(SEQ ID NO.79)

[0181] EGFRvIIIDprobe0080:TTTATAGGTAACAAAAACCCTGGAACCCCAATGTCAGATGTCATCCACTTTTGATTAAGTC

[0182] CAAACATATGACAGATAAACAAAAGATGGTTggctgggctcagtggctcatgcctgtaa(SEQ IDNO.80)

[0183] EGFRvIIIDprobe0081:atcacaccactacactccagcctaggcgacagagcaagactcagtcaaaaaacaaaaaaaaaGTGGTCATTGGAGAATTATT

[0184] GTGTCACCTGTTGTTTTTTAATGTACTAATTTTGAGAG(SEQ ID NO.81)

[0185] EGFRvIIIDprobe0082:TTTTTTAATGTACTAATTTTGAGAGGCTTTTAAATAGAGTGCACTATAGAACTTTTTCTTGG

[0186] CTTCAATTTGCTACAATGTTAATAGAGAATCAGAAACCTTATCCTTATAGATGTTTCT(SEQ IDNO.82)

[0187] EGFRvIIIDprobe0083:TGATTTTTTTAATTTCTGGTGACATTTATGAGTGAGAATAGTGTATTGCCCTGTtttctttcttactcc

[0188] cctttcttcttccttccttgctttctttcttcttcccttccttctttctct(SEQ ID NO.83)

[0189] EGFRvIIIDprobe0084:tcctcgctccttcttttttACAAGCTGTTATGAATTAGCCTTCACAGAGAAAGAAAAATTTTTATAAAT

[0190] AACTGGAAATGAAACTTTGCAAAGGACTGCAGATGAAAAACTTTGTCAAAT(SEQ ID NO.84)

[0191] EGFRvIIIDprobe0085:GACTGTAAAAATATACTATATAATTTTCAAAAGTTAGAAAGTACCAAACACACTCAGTATT

[0192] CATGGTTATACAAGTATGCATACACATGTATTGCTCCCTGAAAAGTGGTGTTGTTAAGG(SEQ IDNO.85)

[0193] EGFRvIIIDprobe0086:GAGTTTTTCTTAGTACGCGGCTTAACATATTTTTTTCTGTAATTTGTTGTTAGTTATAATGG

[0194] GGAGAGAAAACAGGTTAGAGTCTCCCCTCTCAGTTTCACCTTCCATAAAACAGCTAAA(SEQ IDNO.86)

[0195] EGFRvIIIDprobe0087:CTAGACGATCGTCAGACTCCTTCCAGCTGAAAACATCTGTAAAATTAAAAACAAATCTAA

[0196] ATGTATGCAAGATATGTATTTAAACATGCTGGTAATAAGTGTGCTGTCCCTATAATTTAG(SEQ IDNO.87)

[0197] EGFRvIIIDprobe0088:ATGCTAAAACATTGATGTCATaataataacaacacctcgcatttgtacagcacctcatagtttacacaatgccttaacattcttctct

[0198] ctcagcctcctacaaccccacaggattgggat(SEQ ID NO.88)

[0199] EGFRvIIIDprobe0089:agctttccagattgggaggtgagggacccaggctcagagcgattCTGCTGTTGTCCGTAATCACCAGGCTGGTGAT

[0200] CAGTGGGCACTGGGTGCTCTCCTGCTACACAGCACTGTCTCTCA(SEQ ID NO.89)

[0201] EGFRvIIIDprobe0090:ACATGCAGGTCAAGGTTACTTATTCCTCCTTCAAGACGTCATTGGGTTTTTTAGCTATGGA

[0202] TGCCCCATCACTTTTAGTTCTATTTGTGAATCAAAGGCTAAATAAAGTATTCCTCAAAA(SEQ IDNO.90)

[0203] EGFRvIIIDprobe0091:TTTGTTATACTTCTGTTACTAATGCTTAATGTCCCTCACAATTTCTGTATATTTCTGTGTATT

[0204] TCTGCTCTGTTTTGGTTCCTTTCCCAGGTTTCTTTTTTGTTATGAAGTAGTTTTTAG(SEQ IDNO.91)

[0205] EGFRvIIIDprobe0092:ACTCAAGTCTCTTCTGTATGTGTTATAACTGCCCATTCCATAAGATACAGGGCAGTGAATT

[0206] TGTGAGCCTTGAAAATATTTACTTTAGAAATGAGAAGTATGACTTTTCAACGTTGTGTC(SEQ IDNO.92)

[0207] EGFRvIIIDprobe0093:ATCAACTTCTGTAAATTTTCCAGACCTATAAATACTTGCAGAAAAAAAATGAAAGGAGAA

[0208] GGCAACTTGATTTAGCAGTTGGGTCAGTTAGCAATGCCTATGGCAAGCTGTAGTAATTCC(SEQ IDNO.93)

[0209] EGFRvIIIDprobe0094:CTTACATAGATTTGTAAGACTCATTTCTATGATTTAAATGAAGGCATACACTTAACCTCTTT

[0210] AGGGTGTGAAACAGCTTTTACAAAAAGAGACAAACTTAAGAAACAGTGTGGCCCTCCA(SEQ IDNO.94)

[0211] EGFRvIIIDprobe0095:AGAGTGTTCATTTTCCATATCATACCATTTGTAATAAGCTATTCTGGCTGGGATTTACTTGC

[0212] AAGCATTGGCTTTTAAGAAGAGATGGTTTCACACATCAAATTATTCACTTGGAGGCAC(SEQ IDNO.95)

[0213] EGFRvIIIDprobe0096:TTTCTGGGTTGAAGGAATGGAATGGAGAGTGCGGCAGTGAGTAGATCTCTCAGTGACGG

[0214] TGATGTGCCTCTCCCAGAAGAAATTTCAAAATGCAGTGTTCATTTTCCTCCACAAGAAAGG(SEQ IDNO.96)

[0215] EGFRvIIIDprobe0097:AAGAAACTGTTTTGTTATTGTTTATTCCTAACATAGTGGAAACTTTTCAGTACTCTGGCAG

[0216] AAATTTCCCAAAAGCAATTTTCTATTTCATGATTATAAAGTAGCAAAGGAAAAAGTCCT(SEQ IDNO.97)

[0217] EGFRvIIIDprobe0098:GCACTCCAGCTGAGCAATGGATCTCCAGTTGTTATCTAGGTGCTGCAGGTTTAGAGAGGA

[0218] TTGCCAGGAGAACACATCGATTTTTCAGGCCTGTGATGACGTATCTCTTGTTGAATAAGT(SEQ IDNO.98)

[0219] EGFRvIIIDprobe0099:AAACCCTTCCAGTAAACAGACAGTTAGTATATTGATTTCAGGGTGGCTTTAGCCACTGAA

[0220] CCTGTAAGTCTTGCAAAGGTTACTTGGGCAAAAGCATCATTATTTTACCTTCAGTCAACA(SEQ IDNO.99)

[0221] EGFRvIIIDprobe0100:AAAATCTACCTGGCCAAGGCAGAACAGAAAGTTCAGCAATTTGATGAAGTGGGACAACA

[0222] TGAAGAATCAGGTGAGTTGCCTACTTTTTCACTTCACTTTCCACCTTTAGAGATTCTTGTT(SEQ IDNO.100)

[0223] EGFRvIIIDprobe0101:TAGATGCAGAGTAGTGACGTGCCTGGTGTCAGGGAGAGAGTTGAATGAGAAAAGTCCCA

[0224] GAAGGGCAGAAGACTTGGGTGATTATCTGAGTCCATCTTTCCTTATCACATGACAGAGTTC(SEQ IDNO.101)

[0225] EGFRvIIIDprobe0102:TTGAAGTCTTGGCTAGGAATTCTAGGCTTTTAGATTCTTTGGGCAATGGCTACTAAATGTT

[0226] CATAATGTTGCTCAGTTGCAAAAACAAGACATTCAAACTATAGCCAGGGAGATAAGTAG(SEQ IDNO.102)

[0227] EGFRvIIIDprobe0103:TCACGAACTCAAGGCCTAAATTCTGCTGATGGAGCCGATGAGAATTGGGTGCTAAGGCA

[0228] AAGAGAGTTGCCAATATTATATTCTTCGGGGTTTTTTGTTTTTATTCGCATTTTGGAAAAG(SEQ IDNO.103)

[0229] EGFRvIIIDprobe0104:GAAAATATTAGCATTCCTCTGACTTAATATTGAGAAGACATTGGGCACTCTTTTTCCTCCC

[0230] ACACTTGTCTTCTTTCACTAGGTGACAAGGGAAGAGGTAGCATGAGGTGGTGGTCACAG(SEQ IDNO.104)

[0231] EGFRvIIIDprobe0105:GTGAGAGGGGCTGTTGTGAGCACAGGCATGTTGACTGCACATTGGTCACCTAGTAGAAG

[0232] TTTTGCAGGCTTGGTGACTTCTGAACACTGTTTTCAAGGTTGATTTTTAGTTGAGAGAACC(SEQ IDNO.105)

[0233] EGFRvIIIDprobe0106:TCTAGGTACCACGTAATGTTATTAACAGTAGTACTGATCTCACAATCGCCCTATGTCCCATT

[0234] CACAAGATGTTCTGCCAAGCCATAAAAGGCCCAGTTAAGTTTAAGAGAAGTCTCAAAA(SEQ IDNO.106)

[0235] EGFRvIIIDprobe0107:GTAACAGATGATAACTAATTAATACCCAGTGATTTTGAAATGTAGACATCAAACATACCAA

[0236] TTCAGTGGTATCATCCTTAGAGGCAGACAGAGGATGATTAAATCATTCAGCCCATCTCT(SEQ IDNO.107)

[0237] EGFRvIIIDprobe0108:GTCTGAGGACGCAGCTTAGCACAGCATGGTGGAGGCTAAATGGGCCTTAAGGGAAAAAA

[0238] TGATATCTGAAGATGCAATTTATTTCAAAAAGAGTTTGCTCCCGTGAATTTTCACTCTCTA(SEQ IDNO.108)

[0239] EGFRvIIIDprobe0109:TGTAGAACGGCACCAGCACACACTTTTCCTGAGCCTTTGCATGTGTGGCAGGCAGCGGC

[0240] CTGGCATCCTGGGGAACTGAATGAGGACGCAGATGACCCGGACGTGTTCACAGTTTGACAC(SEQ IDNO.109)

[0241] EGFRvIIIDprobe0110:ATCTGACTCCCAGATCAGGGACAGCTAGCTTTGCTGGCTGGTTAAGTTGATGATTCCATCT

[0242] TTGCCTGGTTCTCTGACTGTCTCATGCTTTCTGTTATTACTATTTTGCAGCAGATATTT(SEQ IDNO.110)

[0243] EGFRvIIIDprobe0111:CTGCTCATTTTTCAATCATATATGCATCCTGGATGGCATAGAGTTGATTCTCCTAACAAATC

[0244] AGTGTCCCTTTGTATTTTTTTCTGGCCATAAGATAGAATATATATGTCATTTATTAAA(SEQ IDNO.111)

[0245] EGFRvIIIDprobe0112:AATGGAGAAAATGTTCAGGAGTTTCTTGACTCAGAGAGGGAAAAGGGATACTCAGGGCA

[0246] CTTTTTCAGCCAGGAATTTACTACCTTTGCAGGGTAAAGGGGACTCACCACGCTGGAAGTC(SEQ IDNO.112)

[0247] EGFRvIIIDprobe0113:AAAATAAGCCACCAGTGCCAAGTGTTCAAAGCCCTTAGAATCACAATGCTCTTAAAGCA

[0248] AAGTCTTCAACAATGCTTGAAAACTTCCACTGGTTCTCAGTATGTCCAAAATTGTCATGTC(SEQ IDNO.113)

[0249] EGFRvIIIDprobe0114:TATGAATGATTTTCTCAATCTGAAAATTTTTATAGCAGGCTAAAGAATGAGATAGGTCAGT

[0250] GTGATTCTAGAACTAATCATTAACATTCAATAGATGACTATTTTATTCTAGAAAAAGCA(SEQ IDNO.114)

[0251] EGFRvIIIDprobe0115:GCAACTTTCTATTTACTCTCTATTTTGAGGGTAAATTCTCTGTAAGTAGAAAAAGCAAAAT

[0252] GTGGACATGGGACTAACATATGAATATACAAAGCAAATGTACCGAAAAAATCTTAAGAC(SEQ IDNO.115)

[0253] EGFRvIIIDprobe0116:CTGCCTTGTGGTGTTTTTTGTTTTGTTTTGTTTTCATTAAAGTGACTTGTTAGCCTCTTGCT

[0254] CCCTGTGAAGCACAGGGAGGTGACGTGATGTGCACAGGGCAGACTCTGCCATATGCCC(SEQ IDNO.116)

[0255] EGFRvIIIDprobe0117:TGGCCTTGAACTCAGGGCCCCCTGGGGACTGCAGGGGATGCTGGCCATGCTGAGCAATG

[0256] Cctgtgggtgtcagtttcctcatctgcagaatgagggtaggcctggtgcttatttcatagg(SEQ IDNO.117)

[0257] EGFRvIIIDprobe0118:gtcgcagaggggattcagtgaCAGGGTGGTGTAGAGGCTGGAGCGTGCCCCATGTGTGCACGACAG

[0258] CCTTCCAACTAGGGGAGGCGGGCCTGGGCTCTCACCAGAGAGCCTGTGTTCTCC(SEQ ID NO.118)

[0259] EGFRvIIIDprobe0119:ATGGCTACATGACTTTGCCCCAGACGTCCTTCCCGTGGTCTGGACCCTGGGAAGTCGCCA

[0260] AGAGCCAGACAGGAGAAAGGCTCCACTTGGCTCTCCTCTTTGGTGACCATCCCTTGCCTC(SEQ IDNO.119)

[0261] EGFRvIIIDprobe0120:CATGGCGGGACTCTCAGGTGACATCCCACCAACCCTCACTTTGCTTCCCTGGTGGGTCTC

[0262] ACTTTCCCTCAAGAGTGTTGCTTTTTTGTTTCCTGCATAGTCCTGGGCCAGTTTTGATAA(SEQ IDNO.120)

[0263] EGFRvIIIDprobe0121:CCCTCTTCATTTCACTTCAGAAACCCTGATGATTTCTTCCTGTGCTCTTTTTACCTTAGGAC

[0264] TTTTACTATGACGACTGTGACTGGCCCATTTCTTGTTTTTTTTCTCTTGCTCTGCTTT(SEQ IDNO.121)

[0265] EGFRvIIIDprobe0122:CTCCCCCATCATCACTAAAGCAGACATGGCAATGATGGCCATGCACACTTTCCAAGGGTC

[0266] CAGCTGTAGATCTTCATGGTTCCCCAGGTGCCTGGACCATCTTGTGAGGAGGGAGGCAAA(SEQ IDNO.122)

[0267] EGFRvIIIDprobe0123:CACACCCTGCCTGGAGCACTTGGCCCTTTCGGCAATGTTTTGGCTTCCTCAAGTGAGAAA

[0268] AGAATGGATTTGTATTCCCCCTCTGCATTATTGtttttgttttgtttgtttgttttgttt(SEQ IDNO.123)

[0269] EGFRvIIIDprobe0124:AGAAAAGAATGGATTTGTATTCCCCCTCTGCATTATTGtttttgttttgtttgtttgttttgttttgtattgagacagagtc

[0270] tcacttttttccccaggctggagtgcagtggcccgacc(SEQ ID NO.124)

[0271] EGFRvIIIDprobe0125:acagggtttcaccatgttggccaggTGCCCATTATTATTTGATCTGGAATTAACTGAGCTACTGCAGGAA

[0272] TTGCTTGATTCACTGATGACTGGTGTTGAGCCAGTACACACCCACACCCA(SEQ ID NO.125)

[0273] EGFRvIIIDprobe0126:AGGAATTGCTTGATTCACTGATGACTGGTGTTGAGCCAGTACACACCCACACCCAAGGA

[0274] CTGTGACTGTCTTCTGAGGTCCATCCTCAGAAATTCCTGTCTCTTCACCTAGTGTGTAATA(SEQ IDNO.126)

[0275] EGFRvIIIDprobe0127:AGGCCTGCGCGTGTTATATGGAACTGTAAAAAATGCGCCAACCATCTGTCCTTCCTCTTTA

[0276] TCTGATTACTTATCATTGTTCTCTAAGTTGCAAGTTAATAGACTGATCATAAATTAATG(SEQ IDNO.127)

[0277] EGFRvIIIDprobe0128:CATGCTGGAGACTTGCTGTTTCCTACTAGCAGCATATAAAAGTTATTTTTAAAGTTGTTTTA

[0278] AATCTGTGAGTAAAAATAAATTGCTTTGCTGCAAGAAACACCAAACATGGAAAAGCTA(SEQ IDNO.128)

[0279] EGFRvIIIDprobe0129:ACGGTTCAAAGTTAATAATTTATCTTATGGACATCACTAGTGGCATAGTTGCTTTAAACAG

[0280] TGAGAGGATTTAATAGATATTTGATTTGCAAGTGGGATGAAGGGTGGTCTAACCTTTGT(SEQ IDNO.129)

[0281] EGFRvIIIDprobe0130:CCTGTGTTTACCTTCCATGAGATCCTAGAGGTTGTACAGCACAGTAGTGGCATGTGACAC

[0282] ACTTGAGAGTGCCTGTTCTGTTTGGAAACCTGGAAACTATGAAGGGAAGTGGCCTTCGAG(SEQ IDNO.130)

[0283] EGFRvIIIDprobe0131:CTTAACACATAAGACTTGGGAGGCAAAACCTTTTATTCTCTTTAAATATTCACTTTAGGAT

[0284] AAGCATTTTTTTAGGTGTTAGGAACAGGGAAAACTGTGTGGTTAGGAAGGAAGAAAGAA(SEQ IDNO.131)

[0285] EGFRvIIIDprobe0132:GAAAGTTAACTGTTGTACATTCCCTAGGTAATGTTTTTAAGCATTGTTATTCACTTTCAAA

[0286] ACACATTTTATTTATTTGGACTTAATATTTTGATCTTATTTTTTCAATTTCTTTTAATT(SEQ IDNO.132)

[0287] EGFRvIIIDprobe0133:ATATTTTGATCTTATTTTTTCAATTTCTTTTAATTTAACAGACAGGATGAGTTTTTTTATAGT

[0288] TGTATTACTTAGAAATTATACTAAAAATggccgagtgtggtggctcacacctgtaat(SEQ IDNO.133)

[0289] EGFRvIIIDprobe0134:cagcctggccaacatagcaaaaccccgtcttcactaaaaaaaaaaaacaaaaaaaaaactagccacgcatggtggcaggtgtgcctgtaaccct

[0290] atctactagggagactgagacataag(SEQ ID NO.134)

[0291] EGFRvIIIDprobe0135:aacaaaaaaaaaactagccacgcatggtggcaggtgtgcctgtaaccctatctactagggagactgagacataagaatcacttgaatccaggaa

[0292] gcagaggttgcagtgagcagagattg(SEQ ID NO.135)

[0293] EGFRvIIIDprobe0136:gaaaaaaaaaaaaaaaaggataaagaaaTCATACTAAAAACAAAACAGAATGCTGACCACCTTATAGAA

[0294] ATAGAAATAGTGGTTTGCTGTGATAGCAAATTTTCTTGTTaactttttatt(SEQ ID NO.136)

[0295] EGFRvIIIDprobe0137:tttaaagaattgcacattcacaggaagttgcaaaaaatctactgggaggtcctatcccccttcccccaacctcctccagtagtaacatcttagtagca

[0296] aagttttgtatatttattttga(SEQ ID NO.137)

[0297] EGFRvIIIDprobe0138:tatcattatctaagtttgacatcattatctaatattaacctaagccAAAAGCCCACTATTTTAATTATCTAGTGATGCAGT

[0298] GTTATAGAACTCATAGCCTTTCACAGCATTATTTGGAAG(SEQ ID NO.138)

[0299] EGFRvIIIDprobe0139:TTAATTTTCTTAAGTGAAATGTTTTTGGTCTTTAAGGTTTGGAGGCCATGGAGGCATGAGG

[0300] AGAAATGGGATGAGGGAGAGAGAGCTAAGATAGATAAAGACAGAGATGGGGAGATCCAC(SEQ IDNO.139)

[0301] EGFRvIIIDprobe0140:TGATTCGTTGAACAAACCAGATACTTCCTTATAGTTTTTGGATTAACTTACATGAGCTAAG

[0302] TTTATATTCTGTTCAGATCACAAGTGGTCAAGTTTGTGTGTGTGTGGGGGGGGGGGGGT(SEQ IDNO.140)

[0303] EGFRvIIIDprobe0141:GGGTGTGTGTGTGTACCACTCTACCCATCCTATATTTATTGTCCTGTATTTGGTCTGTTCTG

[0304] CCTTCTTTATTTTCAGGATAGGTGTCCTAaatgagggtctttggaaagctggtgaggc(SEQ IDNO.141)

[0305] EGFRvIIIDprobe0142:catgttgcccgtttcaggtgttccgtgctcaaatgtattcatttcttgaaaaattcagggagtgcacacttttgtacattttcctatgtgtatatgataccatt

[0306] atataaatcttaaaaa(SEQ ID NO.142)

[0307] EGFRvIIIDprobe0143:TATATATGGTTCACCTGAATCCCCAGCCATTTGGTAGAGAAGATAGAAAACCTACAGAGG

[0308] AGGCTAAGATTTTATTAGAAAATTCAGCTTCTCGACGGAGGTATTGGCTTTAAAGTCAAG(SEQ IDNO.143)

[0309] EGFRvIIIDprobe0144:GCAATGCATCTATTCTTTCTTTTGATATAACTAGCTAAAAGATCTCTTAAATTCAAAGTGGC

[0310] CCTCATCTTACTGTTACTGCAATTTACTCTTAATTACAAATTATATAAAAATAGGTTT(SEQ IDNO.144)

[0311] EGFRvIIIDprobe0145:TGAAATACTGTAGCGACAAAGTAACATACCTCTGCTCCATTACACAGATAAAACCTCTAA

[0312] GGAACACCTCCTCTCTTAACAGGCATTAACCAACTGCAGAAACTGCAGAAGGACAGGGCT(SEQ IDNO.145)

[0313] EGFRvIIIDprobe0146:ATTTGGGAATAACACAGCTCCCTTCCTTGTCTGTTCCCTCCCATTGTCAGGCTTCTGTGGA

[0314] GCCATATTCAGAGCAACATAGGGAGGGGGAAGAGAAAATCAACCCCTTGGTGAAGGAAA(SEQ IDNO.146)

[0315] EGFRvIIIDprobe0147:GCTCCCAATTCACAGAGCAAACATGGGTACTCTTGTTTGTGGGAGCTCCCAGGGCCTCCC

[0316] AGCTCACCGAGCATTCTGAGCCCTGATCCTTACACTAATTGTATTATGCAACCATAAATG(SEQ IDNO.147)

[0317] EGFRvIIIDprobe0148:ATGTCTGCTGTACCAGCGGGGACAGTTTATTTTAATAGATTGGTATAACTTGGCAGAATCT

[0318] TATCTGCATGTTTCATCTTGGATTTTTAGCTCAATTCAACTCAATAGGCATGTGTCAAA(SEQ IDNO.148)

[0319] EGFRvIIIDprobe0149:TGTCTACTGCAGACTGAGCACTGAAAAGCTGCTGGGTACAGGGTTACATGGATAGAAAA

[0320] CGTAGCCTCTGACCCCTAAGGAGCCTGTAATCCAGATCCCCATTCTTTCCATCCCATTCTC(SEQ IDNO.149)

[0321] EGFRvIIIDprobe0150:CCAAGCAAGAATTTACCTAATGTGGTTTGCGAGAATTTAAGAGCTGGAAAGGTGGTCAC

[0322] GAGAAGCCGGAATGGGTTCGCTAAAATGTGTCTATATGATTAAGCATAACGTAGCTTTGCA(SEQ IDNO.150)

[0323] EGFRvIIIDprobe0151:GCACTCTTCACAGCTTCCTCAGAGCCTTCCGCACGCGGTGTCTCATTTGAATACTTGTGT

[0324] GAGGATAGCCTCATACCCCTCAGTGAGCTCTTCATGGAGTGATGCAGTAGACAGCAAGCC(SEQ IDNO.151)

[0325] EGFRvIIIDprobe0152:TCACACTTCTATGCTCACGGAAGACCAAATTTGCCttgaaaaatctttatagtctcttcacatttctaagttgacatca

[0326] aaaatcggttaccataaaatcctaatagttgaagagatgta(SEQ ID NO.152)

[0327] EGFRvIIIDprobe0153:atttcaattatttggtaaacctgaccttcattgtcaaagcaattagtcaactcagatttactttctcccagataatagattctgacttcttttttTCTGAT

[0328] TAAAAAACTTAACACCTTC(SEQ ID NO.153)

[0329] EGFRvIIIDprobe0154:CTCAGGAGATCTATCTCAGTTCTGAATGCTGATTCTAACTAAGAAGGATATTTGGCTACAT

[0330] GCTGGGAAGAGGGGTACTGAGGCACGCCGCGATTCCACTCCAGCATTTCCAGTTAGTCG(SEQ IDNO.154)

[0331] EGFRvIIIDprobe0155:GGTGCCTCTGCACTCCCGGTGTTCCGGCGCCCAGTTAGTTGTGTACTCTGGGCTGTCCCT

[0332] ATACTGGAGTCCTAAAACACTTACGACTGCAGATAGGGGGAGGTTTTTCAAAACCTTGGT(SEQ IDNO.155)

[0333] EGFRvIIIDprobe0156:CTGAAAAGCCATagaagggagataggaaagcgggggggtggagccacagtacattcaggtggatccgtttttggaaatagtacaaa

[0334] ctggaggtgaaaccctggaaattgatctgtcgtt(SEQ ID NO.156)

[0335] EGFRvIIIDprobe0157:cacatgcttcatgccgagtccttgtggacccacagagacacactcgccccagtttgaaggctgctAACTTGATTCTGAGGACAC

[0336] CAGTGAGGTGGTAGTGTGCAAATGATGTGTGAGGAA(SEQ ID NO.157)

[0337] EGFRvIIIDprobe0158:ACTTTGGAGGAGTCTCACCCTGCCTGGAGCACGTGGCCCCTAAAACAGCGCAGCCTCCC

[0338] AAAGACAGAAGATGTGGACTAGTGAGAAGCCAGGTATGGTGACTGCTGCTGGATGAAGCTT(SEQ IDNO.158)

[0339] EGFRvIIIDprobe0159:GTCCCACCAGAGGCTCGCTTGTttcattgagcacctactgtgtgcttgtgggatgcaaacacacgtgtggtccctgccctcag

[0340] gTTAATAGGCAGGGGTGGAACAGTTATGAAACTGCTC(SEQ ID NO.159)

[0341] EGFRvIIIDprobe0160:TAAAGTCATTTTCTCAAACTGGGAGTGACAAATGTATCCACTTGGAAAAGATTGAGAATT

[0342] TTATAAGATTTTTAAATTTTTGTTTATTCACATTGAGGAGAATCTAAATTCTTTTGAACT(SEQ IDNO.160)

[0343] EGFRvIIIDprobe0161:TTTAAATTTTTGTTTATTCACATTGAGGAGAATCTAAATTCTTTTGAACTTATGTATAGATTT

[0344] CACCATTTTATAGTAATAAATCAGTCCTCCtgtgtgtgtgtgtgtatgtgtgtgtgt(SEQ IDNO.161)

[0345] EGFRvIIIDprobe0162:atcttaaggaaattaagaggacaaaagaaaagctgctgttttgtatgtatccacatatttaccaGCTGCTTCCCTGCCGGCAGGT

[0346] GCTCTGgttctgcactgcctgttgtcccttgcctg(SEQ ID NO.162)

[0347] EGFRvIIIDprobe0163:aaaatggttgcctccaatattttgctcagttttctgattgtttacagtggcagaggagggtagatctggtaccagttagtaattgccagaggtggaagt

[0348] ctGTGGATGAAATTTGTATAA(SEQ ID NO.163)

[0349] EGFRvIIIDprobe0164:CATGGAACGTTAGTTCCACAGTTAATGCTACTCAATTGGAACCCATGGAAATTATTTTTTG

[0350] GTGAAAAGGGCCCATGCGTTATGAAATTTGAGATCCATCACTTTAAGTGAATGTAGGCC(SEQ IDNO.164)

[0351] EGFRvIIIDprobe0165:CTGGATACAGTGGGAGCTCAGAAGAGCAAATCAGTTGGTCACCTTGCTCAACGTATTTTA

[0352] CTAAGGGCATCAGTAAGGCTTTCTATGACCTGCTCCTTCAATGCTTGGTTGACATTTGGG(SEQ IDNO.165)

[0353] EGFRvIIIDprobe0166:GAGCAAAGATAAACTAAGGATTCTAAGTTCTGTCCTGTGATGCTGTAAGGGGAATCTCAA

[0354] ACCTCTAGGTGGAGGAGTGCAGAGATGACCAGGATGGTGGAAGCCTGCAGGAGAGCTGAA(SEQ IDNO.166)

[0355] EGFRvIIIDprobe0167:CACCTGAAGACACCCAGTGGGAAGACCAGGACCTTTAACGCCCATATCTGCTGCTCAAG

[0356] ACTGGCAGAGAGAAGAGGGTTTGTGATGAGAAAAGGTGGTGAAAGGCACAAGGAGGCACAG(SEQ ID

[0357] NO.167)

[0358] EGFRvIIIDprobe0168:AGCATGTCAGGTCCCATATCCCAAAAGGAATGTGCTTGGGTGAGGGAGAGCTCCTCCATG

[0359] GCTGGAGGCATTCAGAGACCAGGCAGTCGCTTGTGGGTTTGTGATTAGAGTGAGGTTCTT(SEQ IDNO.168)

[0360] EGFRvIIIDprobe0169:TTATAAAGGGAGTGAGAAGAGAAGGTCTGTGGATACTTGAGTGTATCGGTAATTAAGAAA

[0361] TAAATTGTGTACATCCCATTTCTTTCCACATTTTCCTGGGCTGTCACAGTGGCTGCAAAG(SEQ IDNO.169)

[0362] EGFRvIIIDprobe0170:AAAGCAGTCCGTGAACTGAACTGTGATCCCAGACAGGCAAGCACACCAGGAATCTCTTC

[0363] TCAGCTGTTGATAATGAGGGAGCGCTGGGGAGAGAAATGGGGTCCTCTTTGAGTTTCCTCT(SEQ IDNO.170)

[0364] EGFRvIIIDprobe0171:GTGCCGATACCTTTCTCTTTGTTAAAACAGCTAATTAAACACTGAAGCAGTATAGCTCTCT

[0365] TACTATACACTGGTAGTCATAGTTCTCTTACTGTTCTCTTCACTGACAGTTCTCTTACT(SEQ IDNO.171)

[0366] EGFRvIIIDprobe0172:ATACACTGATGGTGACGCAGAAATTCAGAATTCCCCGCATGTGTCCCGGTTTGAAAGCCA

[0367] CTGTGCTTTGCTGTGGATTAGGATCAGACAGTTGAGTCTTGTTCCAACAAGGAAAGTTGC(SEQ IDNO.172)

[0368] EGFRvIIIDprobe0173:TTATTGGAAAGTTTTGCTGCAGGGAGCCTTGAGTTCTGCATCAGGCTTGGAAGTGGGCTC

[0369] TGTGGAGGTCAGAAGGAGGATCCCCCACCCGCAGCCTCAAGAAAAATATGAAAAGTGGAT(SEQ IDNO.173)

[0370] EGFRvIIIDprobe0174:TATGCCTCTGTAGCTATATTGCCTATAAACTTTCTGCAGAATGACAGTATTCATATCCTACAT

[0371] TTTTTCAAAGCGATATTAATCCTGAGACCTGCAGCTAAAGTCAAGTAGAATTTAGGG(SEQ IDNO.174)

[0372] EGFRvIIIDprobe0175:ATAATTAATAGGAGGAAGGTGGGGTTGGAAGATCTGCATGATTATAGTCCTCTGATATAAC

[0373] TGGAAAATTCTTTCCATTAGCAAGGAGCTTTGGTTAATATAAAATGGACAGATTAAACC(SEQ IDNO.175)

[0374] EGFRvIIIDprobe0176:TAGGCAATTTATTTTACTCATTGCTGTATTTTTATTTCAGAGCTGGTTGAAAATATTACAAA

[0375] GTAATATTTTAAAGTGCTTATCTAAACTCTTACTCTGCATTTTATCATTGGGTTATGA(SEQ IDNO.176)

[0376] EGFRvIIIDprobe0177:AATGACTGGGGAAAGACTTTTCTTGCTTTTATTTCTCAGTGTCTACTTATAAACATGTTTTT

[0377] TGAACTACTGTTTTTGTGACAACATGCCTTTTTCCCAGAAAATCTCAGGTTAACATTA(SEQ IDNO.177)

[0378] EGFRvIIIDprobe0178:AATAGGCACTGGATGTTTATCTGATCTTGTTTATAGAAACACAAGAAAATTTTAACCTTGT

[0379] ATATACTTTACTCAATTAACTAGGTAAGAGGTCATTGAAACATTTAGAATTCCACTCTA(SEQ IDNO.178)

[0380] EGFRvIIIDprobe0179:CATTTCAATAATTATCAGGTGAAAGCTACTGCATCTACATCAGAAGATGTTTGTAATTTATT

[0381] TAAGAATAAAATTAGCTATGCAAGAAATAGTATGTGGAGTCCTATGTGGAAATCACAG(SEQ IDNO.179)

[0382] EGFRvIIIDprobe0180:AAACCCTGACAACTTGATGATCTTTCCGCAAGCTAAAAATATCACTCTGGATCACAGCAG

[0383] TAGAGGACTCTGTAAATTTAATCTGTGTGTCTCCTGTAAATAAGTGCATTAGCAGTACAC(SEQ IDNO.180)

[0384] EGFRvIIIDprobe0181:TGTGTCTCCTGTAAATAAGTGCATTAGCAGTACACAGGTGGTGTCAGAGTCAGTGATGAT

[0385] GGATAGAAATTCTACATAAAATccaggctcagtggctcatgcctttaatcccagcacttt(SEQ IDNO.181)

[0386] EGFRvIIIDprobe0182:acgccattgcactccagcctgggcaaaagagcgacactccatcgcaaaaaaaaaagaaGTAAGAAGTTTTACATAAAAAC

[0387] GTGGAGTGAGCCCAAGGTGCCATTTATCCAGCCCATACAC(SEQ ID NO.182)

[0388] EGFRvIIIDprobe0183:GTGGAGTGAGCCCAAGGTGCCATTTATCCAGCCCATACACATCGTACCATGTACAGAGTG

[0389] GACACCAGATAAATACATTGACTGCATGCCACAAACATATATATGTAGGCACCGTTGCAT(SEQ IDNO.183)

[0390] EGFRvIIIDprobe0184:TCAAATACACATCTGCAGCCCTAACACATCTTTATTTGCTAACGAGCATCAATGTATTTAA

[0391] AAACAAACATGTTTAAACTAGTGAATGATTAGATTATAATGATCTTAATTCATAAGTTT(SEQ IDNO.184)

[0392] EGFRvIIIDprobe0185:TCTCATTGGCCTTTTGTATACTTCAATTGTAATACCTAGAAAAACAGTTATGTCCAAAGGA

[0393] GTGAATAGGCCTTATCTGAAACAGGTGAGCGTGACAAGTGTTTTCTTACTTATTTTACT(SEQ IDNO.185)

[0394] EGFRvIIIDprobe0186:TTTCAGATAATTCATCCTTAAAGTACATTAGTTTAAAAGTACTGTTTAAGGAAACAGTACT

[0395] TGGATTAAAACTTGAATCATTGTTAAGGAAAACTATACCTTAACTTCATGTAATCACAA(SEQ IDNO.186)

[0396] EGFRvIIIDprobe0187:TTAAACCTCTTCATATAGAAGGATCTAAGAATTTTCTGCAGCATTCACCAGCACCAAAAA

[0397] GCTCAGAGACATATATTTCTTTCTCTGTATATGTATTTTAAATTCAAGTTAGTATAAATT(SEQ IDNO.187)

[0398] EGFRvIIIDprobe0188:GACAGGCAGGtcagagtaatatatgatcttctgagtccccttagtaattaaaagaaatgattatttttgcatgaaatatgataaagtgattttaa

[0399] gtGCCTGATAAAAAGTCTTAACCAT(SEQ ID NO.188)

[0400] EGFRvIIIDprobe0189:GACAACCATTAAAGATTACATCAAAGAAAAATAAGTTTGACTTTCATTTACCTTGGAAAC

[0401] AGCTATTAACTGGTAACCTCAAGAAACACCATGAAGAGTCAGTTTGCTCCACACATGTCT(SEQ IDNO.189)

[0402] EGFRvIIIDprobe0190:TGTAAAAGTCAAATAACTGGTGGTTATCCAGTAATGACAAGAGGTAGAAGTTACATCCTT

[0403] GCTGTCTGATTGAACCTTCCCAGAGCTGGCACAAGGCTGGGAAGACCATAGGTGCTAAAT(SEQ IDNO.190)

[0404] EGFRvIIIDprobe0191:GAGGAACTACTTAAAGAAAGAAAATGGAATTTCACGGACAAGAAAATCCATGTCCATTT

[0405] GGTTCTGTGACCCACATCCTTTGTATCCTATGCTTTTTTACACTTGGTACATGGTTGCAAG(SEQ IDNO.191)

[0406] EGFRvIIIDprobe0192:ATTGCCCCTGTTTTCTACTTATAGTTCCATGCAGCATGGATGTGGGAAAAAGTCTCCTCTG

[0407] CAAAGGGGGTTAATGCAGGTCACTCTACGTATGTGCACGAGGTCGTTATAAAGCTCGAA(SEQ IDNO.192)

[0408] EGFRvIIIDprobe0193:AATATGGGCTCACCAACCAGGTGATTTTTTTAATTATCCAACCAGAAGACATAACATATAG

[0409] GGGAATCAAAAGAAATCTCTGAGTAAAATAATGATAACAGGTCAAACTTTGCGGTCCCA(SEQ IDNO.193)

[0410] EGFRvIIIDprobe0194:CGTGAGGCTGGAGATGCGTATTGTCTTGACTTTGCATCTACAAGTTTAACAAATGATGCTT

[0411] TCTCAGTTTACCTCTGGAAATGGAAATTAGCATTGCAAATGACTTCATGAGGAGGTAGA(SEQ IDNO.194)

[0412] EGFRvIIIDprobe0195:AGCTATCTGTGAATTTCCTTTCGCTGTGTTTACGATAGACTCTCACGTCTAGATGTGTCAT

[0413] GTATTATGTTAAATTGGTATGTCTTGAAGTTATAAAGCACAGCCCTCTATAAGTATATA(SEQ IDNO.195)

[0414] EGFRvIIIDprobe0196:TATTCCACCTCTTTCAAATCGGATGGTACCTATCCTTCAAACTGCTATTTAATGACTGTCTG

[0415] CTATGTTCAAGGCACTGCTCTCAATGTTAATACTTgatgagatcgggcgcgttcaagg(SEQ IDNO.196)

[0416] EGFRvIIIDprobe0197:tggcatggccgtagacTCAATGTTAGTATCTGAAATATGGCCTACGAGCTGAGTTGTGAATCAAGT

[0417] TAATAGATTTTCGGAATGTTAAGGTCTAAACcagtagctcttaactgagacaat(SEQ ID NO.197)

[0418] EGFRvIIIDprobe0198:cctgtcctcatctcacctgggagacatctggcaatgtttggagaaccttttggttgtcacactggggcatctagtgagtagaggtcagggatggtgg

[0419] taaacaagtttttttgtttgttt(SEQ ID NO.198)

[0420] EGFRvIIIDprobe0199:cctgggagacatctggcaatgtttggagaaccttttggttgtcacactggggcatctagtgagtagaggtcagggatggtggtaaacaagtttttttg

[0421] tttgtttgttttgtttttgaga(SEQ ID NO.199)

[0422] EGFRvIIIDprobe0200:cccccttcttggcctcccaatatgccgggattacaggtgtgagccaccgtgcccaggctaacattctttaatgcataggacagcccccaccataca

[0423] gaggaatccccagcccagaatgtt(SEQ ID NO.200)

[0424] EGFRvIIIDprobe0201:aatagttctaaggttgagaaacccAAGGTTAAGCCAAGTCAACTTATCTATCTTCTTTAAAATTGCATAA

[0425] GAATGCAGTCCTGTTCTTCATTCCTCTTGCTTTGCAGTTAATGATCCTTT(SEQ ID NO.201)

[0426] EGFRvIIIDprobe0202:GCCTGGACTTTCTAAGTGCCCAGAAGAGCAACAGCCAGCATGCAGGATGGCATTCCTGA

[0427] CCAGTTGCACTTGGCCTAGCATTCCAACCTCACCTGCCTCAGCTTGTTCAACCTGAAAACC(SEQ IDNO.202)

[0428] EGFRvIIIDprobe0203:TACCAAGTGAAAGCAAGAGCCACGTGAAGACGCCTTAGTTATATGCACCCACCCAGACA

[0429] CTTGCTCAGAAAGGAATCAGTGGGGCCCTGGCCTTAGAAACTGGCTCCTTCACTGCTGTAG(SEQ IDNO.203)

[0430] EGFRvIIIDprobe0204:AAACAACATAAATTTAACATAAAACACGTGCTTTTCTTTTTTCTTCTTACTTTTTCCTGTCT

[0431] TGGCAATGCAAGGATGCCATTAGGTAAAGAAATCCTTCACCACACTAATCCTGCAGAG(SEQ IDNO.204)

[0432] EGFRvIIIDprobe0205:CCAGAAGAGAAACCAGCTTGTTCTAACCCAGCTTTGTCATGGAGAGAAGGCAGCTGCTC

[0433] CAGTCTGAACTATTCTTTCTTTTGGTAGCAGCCTGCCCAAGGGTGAAAGTGTGTTTAATAG(SEQ IDNO.205)

[0434] EGFRvIIIDprobe0206:TTTGAATTACACAAGTGAACAGTAAATGTATGCCTGTTTCTGCTTTATGGGACTTTGAAAT

[0435] AATGTTGTTTGTGCCAAGGTTTTAGATTACTATACCTAACAACCTAGAAAAAGAAATGA(SEQ IDNO.206)

[0436] EGFRvIIIDprobe0207:AAAGGAAGCCTTCTGCCAGGCAGAGGTCACTACGGGCCTGGAGCTGGGCACCTGACTC

[0437] AGCAGCTGCCCAGATCCCCAGAGCTGAGAAGTCACCATGCATTTGTGGTGCTTCGAGCGAGT(SEQ IDNO.207)

[0438] EGFRvIIIDprobe0208:TACCAGAGTCCTGGAACAGAGCAGCACACCTGCGGGGTGTCCCCTTGGCATTTGGGCAG

[0439] GGCAGGTGACCAAGGGTCTTGTTGGAACTGAAGTCCAGCTTGAAAAGCAAATCTGGTTGTG(SEQ IDNO.208)

[0440] EGFRvIIIDprobe0209:AGCTAGAGTCCAGTAACACTTGTTTCCCGCCGCCCCCCGCATAACTCGTGTGTCCTAAAA

[0441] TACAATAATTTCTTGAACTTCAGTCACTTATGCCTATAAGCGggcatacaacaggggcac(SEQ IDNO.209)

[0442] EGFRvIIIDprobe0210:aataaatgtttgttaagtgaatgaatTCTTTCAGAACTAGATGGGATCTTAGTCCAACTCTCTTATTTAACG

[0443] AGGTCCACAGAGGTTCTGCGATTGTCTAAGAAAGAAGGCTGTGTTCAT(SEQ ID NO.210)

[0444] EGFRvIIIDprobe0211:GGCCTTTGTTGTTTACGTGGCCCTGTGATTCTCTTGGCTCCGTGAAAGTCCTGATGCAGA

[0445] CATTCCGGCCATCTAGAAAGGCATGCAGACAAGCCATCCAGCTGGCATGATCCTGAGTCC(SEQ IDNO.211)

[0446] EGFRvIIIDprobe0212:AGCtttctttaaaagagcttccaaaactgcttaagctttgactgcacaaaacctgcatcACCTCCAGTTGAGAAACTCAAGA

[0447] GAATAAGTAAGTTATGGAGTTGGAGACCCCAGCTTAAC(SEQ ID NO.212)

[0448] EGFRvIIIDprobe0213:TACTAGTTTTAAAATAGTGAAATCAACATTTTCAAATCTTTGACTTCACTAAGATTTAATAA

[0449] AGTTTATTAATCATATATTATGAGTTATTGCTCTCTCTTTATGTCTGTAATGCAGTTG(SEQ IDNO.213)

[0450] EGFRvIIIDprobe0214:CTCCTCTCTGTATAAATTAATAAGTTTTAGAGATCCAAAATGAGAATTTTAAAATAAATTAC

[0451] GTATATTTTAATCAAGTTTAATTTGACTATATCCAGCTAAACAATTGATTGAACTTCA(SEQ IDNO.214)

[0452] EGFRvIIIDprobe0215:CTTGCTTTTCTATGACAGGTTTTTTGTTCTTAGTAAAAgaccccagttttctcacttgtgaacagaaggggttaga

[0453] cttcatgacagctaaggttccttccgtctctaacaAAAGTGGCC(SEQ ID NO.215)

[0454] EGFRvIIIDprobe0216:TGAAGAGAGGCTTCTAGACTATACTCACGGTGGGTTCTTGGGACCTCAGAGTCAgctccatca

[0455] cttaagtggctgtgtgattgagtggagacacctcaatctctttgtgcctcagtttcc(SEQ IDNO.216)

[0456] EGFRvIIIDprobe0217:tcacctgtcgagtgtcaacatgatggcacctaaagctgttgagacttcagaaaggtaatgtgtgaaaagtgaaaagtgcctggcatccaggaagta

[0457] ctcaataaataccaactattttat(SEQ ID NO.217)

[0458] EGFRvIIIDprobe0218:tGCTGCAGCTGTTCTTATAGATGTGATTTCTAGAACATTGCCTtctaatagggtagccatgggccacaattgtt

[0459] ggctgttcggtgtttcacatatggttagtccaaactaagatgtgtt(SEQ ID NO.218)

[0460] EGFRvIIIDprobe0219:gtgagtctcaaatacacactggattgtgaagacttaggacaaggaaaacaatgttaataaaatctcattgataacttttaaattaattacatgttgaaat

[0461] gaaaatatttgggacatatt(SEQ ID NO.219)

[0462] EGFRvIIIDprobe0220:gagttaaataaaacaggagattaatttcttctgtttctttctactttttttattagtgtggctactcaaaaatgtgacattatgtatgcatctcgtattacatttc

[0463] tattggacagcAGC(SEQ ID NO.220)

[0464] EGFRvIIIDprobe0221:GCTCTAGACAGTACTATGGGTAGTATCTGTGGGGAGGTTCTCAGAAACATGTCGCATGCT

[0465] CTTTTAGAACCTTAAAGTATTCCTAGTCTCCTCTACTTCCAGCCCTTGGCTCTTGGGCCT(SEQ IDNO.221)

[0466] EGFRvIIIDprobe0222:CAGTCTTTTTACTTTTGCGGCTGTGTTTCTCTGAAGGCTTGGCATTAGTAGATTGAAAAGA

[0467] ATAACCATCTAGGGAAATGTGAATTCAGTTTCTTTCTGACATTCTGCTCTCTACAAGGG(SEQ IDNO.222)

[0468] EGFRvIIIDprobe0223:GATATTATGTACACATAAACCTACTTCCAAAATAATGAAGTGAGGCCTAATTCCTTACTCTT

[0469] CAGAGAGCCCACTGTGGAAGTGTCACTGACCTTGTGTATGGGCTGCCCTTCATGGCTC(SEQ IDNO.223)

[0470] EGFRvIIIDprobe0224:TGGGAGTCATTATAAAGGGCAGCATTTGGCGTGGTGCGTCCTAAGCCAGTGTTTCTCGGC

[0471] TCTGTTCCTTAGACATGTGTTAGTGTTAATAGATGTTCTTGGAAAAAAAAAAAAAAAACA(SEQ IDNO.224)

[0472] EGFRvIIIDprobe0225:GCATTCTGAGGTCAAACATGCTCAGAAAGCTTGGAATCTGCACTACGCTTCTCGtacacatttca

[0473] tattaaagattttggaaagtcctgcaatacagagccctgtctaatattgccacaa(SEQ IDNO.225)

[0474] EGFRvIIIDprobe0226:cccacaattgctcaaaTGTAAATAGATTTGAGTTTATTCACATTCAGatcacctcttaaggccccacctcccaatgctgt

[0475] cacaatggcaattagatttccacatgagttttggaaggga(SEQ ID NO.226)

[0476] EGFRvIIIDprobe0227:cattcagaccacagcaGGGGAAAGCAGGGTACTTGCTGCTTTGCAAGTGTGTCCACATCTAATTAA

[0477] TAGTACAGTTCTTACTCTTGGTGTGTCCGGTGATATTAAAAATTAATGTGCCTT(SEQ ID NO.227)

[0478] EGFRvIIIDprobe0228:ATTTAGATAAGTAACATAAAAATCACAAAATGTATGCCTTAGATTTATATGTATTTATAACTA

[0479] GTCTATTTCCTGAAAACAGTTGAGACACCTTGTAAAAGTTACCGGTACGATAGGGCC(SEQ IDNO.228)

[0480] EGFRvIIIDprobe0229:ATTCCAACAAAGCTGTAAAGTGGTGATAACACAGTCATAAAGAAGAGGAGATAGCTCTG

[0481] GGAGAAAAGGTGGCCCAGAAACCAGCTCTGAGCCTCATGGCTGCAGGCAAGGTCTGCAGGT(SEQ IDNO.229)

[0482] EGFRvIIIDprobe0230:TCCTGGTCCTGATTGCAGGCCATTTGCTGCCTTGAGTGGTGGTTACACAAGGCCAGCCCT

[0483] GGGGGTATCACCCAGAACACCTAGTACACGAATTTCAGTTTAGAGGACGAAGCATTACTG(SEQ IDNO.230)

[0484] EGFRvIIIDprobe0231:GAGTATTGTTATGCAGGAAAACTTTTTCCTAAAAATGCCCTGAAAAGAGAGTAGCCTAAT

[0485] GCATTCAATCAAAATGTTTTTAAGTGGAAAACATATTGTGTGTACTTGATCTGGCCTGCT(SEQ IDNO.231)

[0486] EGFRvIIIDprobe0232:GTAGCCTAATGCATTCAATCAAAATGTTTTTAAGTGGAAAACATATTGTGTGTACTTGATC

[0487] TGGCCTGCTGCTTTTAAAAGATTAAAACTGGgactgggcatggtggctcacacctgtaa(SEQ IDNO.232)

[0488] EGFRvIIIDprobe0233:gggcaacaagagtgaaactccatctcgaaaacaaacaaacaaacaaaaaaacaCTGGGGCCAAAGAACTCTGTGTGCT

[0489] GTATCACCTAACCACATTTCATGACACGGCTAGAGAAGAATC(SEQ ID NO.233)

[0490] EGFRvIIIDprobe0234:ATCACCTAACCACATTTCATGACACGGCTAGAGAAGAATCATGCAAATAAAAATTTCCAA

[0491] CATGTTCGTAAACTGGGAAAGTATTTCACTGGGGAGTGAGCAGAAAAGTAATACTATAAC(SEQ IDNO.234)

[0492] EGFRvIIIDprobe0235:CTCTATATCTAGACAAATGTGAATTCAGTTTCACATATAAATATATAAGTGAAAaaatatataaatat

[0493] aaataatatgaaataatggttatctcaccactttctacatcttttgtgaata(SEQ ID NO.235)

[0494] EGFRvIIIDprobe0236:ttttatagtgctcaaatatattagtgcactagtatatgtacattacattaaataactaatcatttattaggaggatgtgcttgttttttgctaataaagatgata

[0495] ataaaaaaaTCCTTA(SEQ ID NO.236)

[0496] EGFRvIIIDprobe0237:GACCCCCCCTCGGTTTGTTTTCAGTTAGGAATTAGGGATATTTATAAGAATATCTTTAAATG

[0497] ACACATGCCTTGCTCTGGGACGAGGCATCTGCATGGGTGACACATATGTGTTGTGTGT(SEQ IDNO.237)

[0498] EGFRvIIIDprobe0238:ACAGGCTCCCAGCATTTCCAGGGCCCTGCTCAGAATGTAGGCCTTACTGATTCTTACAGA

[0499] GTTACAAGCGCTGGTGAGGTTGGCGAAGTTTAGGTAAACACAGCTGGGAATGCCCCATGG(SEQ IDNO.238)

[0500] EGFRvIIIDprobe0239:CCTCTGGGTGACTTTGGACATCACTGAACTTTACCCTTAGAGATGCATACCTGCATCTTTT

[0501] TTACCCTGATAGGGCCTTCCATGATGCTTTCAAAGTGTTTTTGTCTGCTTTTCGGTTAA(SEQ IDNO.239)

[0502] EGFRvIIIDprobe0240:TAGACTTTCACAGTAGCCAATTGaatatattggttaaatgcatctctttatacacagactggattcaaactgaggttgtgtctctcc

[0503] ctggctgtgtgacgttgggtatgatccaagtgt(SEQ ID NO.240)

[0504] EGFRvIIIDprobe0241:cagattactcaacttcaaaatgaggacagagcctttcccttctagggctgccaggaacattgaatgagagagtgctggcagcttagtacagGTG

[0505] TTCATTGCTCTTGTATGGTACTGTCT(SEQ ID NO.241)

[0506] EGFRvIIIDprobe0242:GTGGCACGGCTAGATAAAATACAGTAGCCACTGATTCAAATTTCAACTGAGGAGTAAAAT

[0507] AAACTGAATAACTTAGAAAAGTTTTCTTCTTTTGAATGACTCTAAGAATTTAAGGAGCAT(SEQ IDNO.242)

[0508] EGFRvIIIDprobe0243:GTGAGTGTTGATGGCTCTAAAAGGGTAACAGAGCCCAACTAGCTCAGTTCTCAGCATGA

[0509] AAATAGTCATATGGCACAGACTCAGTGGAGTGGGTGCACTTCAATAACTGGAAGCACAGAT(SEQ IDNO.243)

[0510] EGFRvIIIDprobe0244:GCCCTACAGCAGCATCAAAGATGGCACTCTAAACTACTTTCAATCCTTTAAAATAAATGG

[0511] AAACGCACATTTAGTATGCATATGACAACACGAAGGACTTCGATTTTGCTGATGCAATAC(SEQ IDNO.244)

[0512] EGFRvIIIDprobe0245:AGTTTTACAGGATTTTTTATACTCAAATTAGTAAAATTCTGTATTGCATCCAAATTATAAATT

[0513] ATAATATCATCTAGATTGGACATAGGAATAACGACCACTGGTATCTGCCCAGAAAGC(SEQ IDNO.245)

[0514] EGFRvIIIDprobe0246:TCTACCGCCTGTTTATAAGCTCCTGCAGGAGACACAAAAAGAAGAGAATTTGAATATAAC

[0515] TTGAAATGACCGTAATCTCCTGCCCCAACTCATTTCATTACCAAACCGCCTCTTTCTTCA(SEQ IDNO.246)

[0516] EGFRvIIIDprobe0247:TTATTTCTCCTGAAGCACAAATCTATAGAGAACTCAGCTGCCAGTCTCTCCCACTGCACTC

[0517] AGCAGTGAAAGGGTTAGGCCTAGGCTTTTCAAACAGACCAGTGCTTGTATCAGCCCTTA(SEQ IDNO.247)

[0518] EGFRvIIIDprobe0248:AACATCTCTGGAGAAGGAAATGGGATCCTTCTTTGGTAATTCATTTTTGACAGTTGGGGA

[0519] TTAGGTGTTCTGTATCTGGGGGGCCTTGCTGTCTTCTCTCCTCCTCCTCCCACTGCAGAC(SEQ IDNO.248)

[0520] EGFRvIIIDprobe0249:CCTCTCCTCCCCTCCCCTCTCCAGCTCTCTGATGACTGCTTCATGCTCCTTCCACCTGAGG

[0521] ACTGCCAGCACAGCCTATTGCAGGAACAGCCAATGAGGGGCTGGCTGTGCTCttttatt(SEQ IDNO.249)

[0522] EGFRvIIIDprobe0250:tataaaattataaaCTCAAGCAAAATCTAGACTATGTGTCCCCAAGATCAGAGGAGCACAAATCCC

[0523] TTGCTTACAGATTGCATGGGGGGCACATTCTTTAAAATTGGTCCCTGATCTAGA(SEQ ID NO.250)

[0524] EGFRvIIIDprobe0251:CTCTAGCCTGAGAATCATCTTTAAGTTCAGAATTTCCACTCATGACCTCACATCTGTGGGC

[0525] TCCCACATTGTCTTCCAAAACACACATGGCATCTGGCATCACCTTCACCCCCACCCTCA(SEQ IDNO.251)

[0526] EGFRvIIIDprobe0252:GAGCCTCATCTCCCTGCAGGTAGATAGTCAAGGCAACCTCTTCACTCTTCTGCCAAGCCT

[0527] CCTCTCCTCAGCTCTTCCCTTCCTCTCTCTTTTTGAAAATAtttttaattgtggcaaaat(SEQ IDNO.252)

[0528] EGFRvIIIDprobe0253:atacacaacataaaatttaccatcttaatcatgtataaaagtggagttcagtggcattaaatacattcacgttgttctatagccataaacaccattcatct

[0529] ccagagctcctttcatctt(SEQ ID NO.253)

[0530] EGFRvIIIDprobe0254:gcaaagctgaaactctgtccccattaagcaatggctctgttttcctccgttcccccagcccctggccaccatcctcagttttctgtctctgtgagtttga

[0531] ttactctaagcacctcttat(SEQ ID NO.254)

[0532] EGFRvIIIDprobe0255:aagtggatcatacaatgtatctgtctttttgtgactggcttgtttcactttccataatgtcttcaaggttcatccacgttgcagcatatggcagaacatctg

[0533] tccatttccaggctgaat(SEQ ID NO.255)

[0534] EGFRvIIIDprobe0256:ggtactcttttgtacgtgtggaccacatttcatttatccattcatccacgggagggcacttgggttgcttctgctttttagctattgtgaataacgctgcta

[0535] tgaacatagctgtaTGCC(SEQ ID NO.256)

[0536] EGFRvIIIDprobe0257:TTTGTcttttaaagcccaaatctgatcaagtcactccccagcttaaaaccttccactgctccccaGCAGTGGGATAAAGGCCA

[0537] GTCTCCCCTGTAGGTCTCTCCCGCCAGCCCTGCTCAG(SEQ ID NO.257)

[0538] EGFRvIIIDprobe0258:TCTTCTTGCTTGTCATCCTTGGCTAGGCCTTGCATTGCCATAGCCCTCTGCCTCTGTTCAC

[0539] GCTCTCTCATCTTGGAGCATGAGCCTTCCATCATCTCTACCAGATGAACTCTCATTTCT(SEQ IDNO.258)

[0540] EGFRvIIIDprobe0259:TCTTTCAAAAAATAAAAAACCCAAAAAACCCAGAGATCCCAACTGTCCTGGTGTCTGCA

[0541] TAGTCTGCAGCACACGCCCCCTCCATGGCCCTTCCTCCATAAGCAGAATCACTCCTCACTG(SEQ IDNO.259)

[0542] EGFRvIIIDprobe0260:TTCCTGCAGCACCTCCTGTGTGCCCACACAGCTGTCCTGCGGTGGGCTGTGTGTGTGAGT

[0543] GTGCCCCCTCTAGGACCTGAGCTCCTTCTGGAGGGTGGGCACAGCATCCATTCATTCTGG(SEQ IDNO.260)

[0544] EGFRvIIIDprobe0261:GAATCCTGGTCGGCACCATGCTAGAACTTCTGCAAGTGAGTGCCTTTGGTGCTGGCCCAT

[0545] GGGAGAGCTGTTGGTAAGGCATACTTTTGCAGATTCCAGTTGCTGCTGAGGTTGTTGCTC(SEQ IDNO.261)

[0546] EGFRvIIIDprobe0262:TTTGCACAAGTTTCTTCTAGTCACCAGTGAAGTGACATGTGTGGCAGGCATGGCCCAGG

[0547] GAGGCTTTTTCATAAAGAAGAGGTTGAATCTTTGGGgctgtggtttgaatatgtccctcaa(SEQ IDNO.262)

[0548] EGFRvIIIDprobe0263:gcttatgtgttggaaacttaatcccaaatgcaatagtgttaggaggtggggcctaatcacaggtgattaggtcataaggctctgccctcatggatgg

[0549] cttaacatgtttagtgaggcagt(SEQ ID NO.263)

[0550] EGFRvIIIDprobe0264:gggttagctattgtgagagtgggcttgttagaaaattgagtgcagccccctcttgcttgctggctaccatgctctcttgcttttctgccttctgccgtgg

[0551] ggtgacacagcaagaagacc(SEQ ID NO.264)

[0552] EGFRvIIIDprobe0265:tctgccgtggggtgacacagcaagaagaccctccccagatgctggcaccatgccctgggactttccagccttcagaaccacgagccagacaaa

[0553] tttcttttctttataaattacccagtc(SEQ ID NO.265)

[0554] EGFRvIIIDprobe0266:gactaagacaATCTTCTTTCATCAAGTTAGGGTACCAACCTTTAAAGACTGCCAGTCCAAGGTT

[0555] AAAGGAAACTTTTCAAGAGCAGTCCAAAcatgatctggccctcagctactctccag(SEQ IDNO.266)

[0556] EGFRvIIIDprobe0267:ggtcatgccaccctatcacccactggctcacacagacgctgaccactgcttagtttctcaaactgaagttttcctcctcagagcttttgcaaaacctttt

[0557] ctttgcctggaaaactcccc(SEQ ID NO.267)

[0558] EGFRvIIIDprobe0268:ccacaaatctttagttgtaggttccttctcatcttgcagAATTATTAGTTTGCTCTTCAAATAgtctctccagctagactatcaact

[0559] ccaggagggcagagttcttcttcgcttccttca(SEQ ID NO.268)

[0560] EGFRvIIIDprobe0269:cccatgtgcccactgagtccagaacTGTATAGCAGTTTGATTGAAAAAATCcacagggtggaggatgagaggaccctg

[0561] gatcccagcctcacagcctcttacttcacctgtgtgattttg(SEQ ID NO.269)

[0562] EGFRvIIIDprobe0270:gtcaagtcctttattcttcctgggctttagttttcccttatctaaaatatgagAAAAGTTCCCCTCTCCTGGGTATTCTGGGA

[0563] GACTCATGTAAAAGGCACTGAGCCAGTGCAGCACATC(SEQ ID NO.270)

[0564] EGFRvIIIDprobe0271:TATGACCAGGAAGGGTCAGCTTCCTGCCTTGCATGAGACACACATTCCCTTCTTCATGCA

[0565] CAGTTATTCATGAGTTAAATATGTATTGAGAAGTGGGTTCTCAGGAGATGATGCATCCAC(SEQ IDNO.271)

[0566] EGFRvIIIDprobe0272:AGCATTGTTTGTATGCCTCTGTCTTTGATGTCCCTGCCTGAGTCGCCCACTTTAGAGCCCT

[0567] TCTGTTCTTCAGAAACCAGACTTTTCTTTCAATAGTTTCAGTaatcaatcgatcaatca(SEQ IDNO.272)

[0568] EGFRvIIIDprobe0273:atcaaccaatcaaCAGTGATAATAATCATGAGTGAGCCCCTGCCCGTGCTGGCTGTGTCCTGCTG

[0569] AAGGCACACTAAGTGCTGCCCTTCCCAGAAGCCTCAGGAAGCTTGCGAAGCTCAG(SEQ IDNO.273)

[0570] EGFRvIIIDprobe0274:GTGCATGGATGCCTGGTGGAATGAGGAAGGGATGCAGCCAGGTAGAGAAATGCCCTGCC

[0571] ATCACTTGCATCAGCATCTGTGAAGAGCTGGCCAGGCTTTTGCTCACAGTGGTTGACACAG(SEQ IDNO.274)

[0572] EGFRvIIIDprobe0275:TCAAGGAGCAAGGGCCCCGTAGGAGAGGGGAGTCAAGGGCTCCGGGTGGGAATGGAGC

[0573] TGGGGGCTGATGCTGGCTTCTGGAGCACTGTAATGTGACTGAGAAAGGTGAAGGAGCCGTTC(SEQ IDNO.275)

[0574] EGFRvIIIDprobe0276:TGAAAAAGAAGAAGGCAGGAGCTCGCACAGCTCTTGACTCATCTTGACTTCTTTTTCCT

[0575] GCTTCATCCAAGCAGGTCGACTCTCTCGTGATCTCAGAGACAGAGTGAAGTCATGAGTGGG(SEQ IDNO.276)

[0576] EGFRvIIIDprobe0277:AGGGGAGCACAGAAAATAAGACCTTGATTCCCAGCATTGGGAGACTCCCTGCTCCCCTG

[0577] AGTCTCGGAAAATAGCACCCTTCAAATGTTTTAGGGATCCAGATTTGATGAAGAGATGTTA(SEQ IDNO.277)

[0578] EGFRvIIIDprobe0278:TTTTGGCTTTTAGATTCTTAGGAGAGATTTGTCTTTCTCAGGTCAGGAAGAAAATGCTGCC

[0579] CGCTGCACATTCTTCGGGACAGACTCTTTTAATTATTACTAGTTTAATGTATGTTTTGC(SEQ IDNO.278)

[0580] EGFRvIIIDprobe0279:TTAGTTAAGGAAAACCCCTGTGGTTTCTTGACGTGCTTCAGTATTCTAACTCACAGCTGAT

[0581] TCAGTTCAGGGGGCTGGGGAGATGTCCTCGACCTCTGGAAAGGAGGGTGCATCTCTAGA(SEQ IDNO.279)

[0582] EGFRvIIIDprobe0280:AATAAGGCTAAGTATGCCACTGACACTGTCTGCATAAACGTGTGTGATCTCAGGTCCAAA

[0583] GGATGGGGCCTGGTCTAAGCCAGGGACGTGGGAAATCATTTTCCTGTGGCAACTTGTGAA(SEQ IDNO.280)

[0584] EGFRvIIIDprobe0281:GACCATTCTGTGACCTTGGTGTCTCTGGGCCTTCTCTTAGATTTTCTAAGTTGGCTAGTCA

[0585] GTGGAGCTGCCATCCCTCCTTTGCCCATGTTCTACTCCCAGAGTTCCTCCAAGAAATTG(SEQ IDNO.281)

[0586] EGFRvIIIDprobe0282:CGGAGCAATGCCTGTTTCATGAGAGCTGAGTTTGCTGTGTCTTCCACTTAGAAACAACAC

[0587] TGTGGACCAGGAGGACACACAGCTCCCAGGGCCATCACCACACAAAGTGAAGGCTGGTGA(SEQ IDNO.282)

[0588] EGFRvIIIDprobe0283:ATCCGAGGCTTCTAGCCCTTGCCGGGCCAGGCCCGCAGCACTCCGCTCCCCAACCCAGC

[0589] CGCTGCTTTGTCGCAGGAACCTCAGCAGGGCAGGGTGTTTCCTAGGAGGACATCCGATTCC(SEQ IDNO.283)

[0590] EGFRvIIIDprobe0284:CAGCCATTCCTTTCAGTGAATCACCTGAGCTCACATTCTTTTTTCTTTTATTTTTGAAGCTC

[0591] TTAGCCAATCTGCTTCGCGATGAACCAGTTTTGCTTGAAGCAGACAAACCCGATTGTC(SEQ IDNO.284)

[0592] EGFRvIIIDprobe0285:AGGAGACAGTGATGATTTCTTCAGTCTCTGAGGAAGAGTTTTCATTTTCCCCAATTCGCA

[0593] AAAAAAGTCAGGTCCCTCCCTCCCTCCCTCTCCGTAGAATATTTTCCATGTGTGTTAACA(SEQ IDNO.285)

[0594] EGFRvIIIDprobe0286:ATGGCTGAGCGTGGTAGATGCCAGGAATTTCTGTCAACCCTCAAAGAGGAAAGCCCTGC

[0595] CTAATGGTCTGCCCGTTCTTGTTCACTCCCTGCCCCAGGCTCCCCACCCGCCTTCTTTCTG(SEQ IDNO.286)

[0596] EGFRvIIIDprobe0287:GAAGGTATAAAGGCTCCTGCTTATACCTGGCACTGCACGCTTCGCTCCCTCTGATCTCCTG

[0597] ACTGTCATGCCCAGTGTCTCAGCCTATCATTCTACCTCTAACTCGACCTTGAGTGACCT(SEQ IDNO.287)

[0598] EGFRvIIIDprobe0288:TGAGCAAGTTTCTCAGGATTCCACCTCCAAGTCACTCTCCCTTTGGGATATGCAGCACTA

[0599] AGTTAAGCTTGCCTGGAAAACATCACTTGAAGCTGGAAAACCACTTTTAACACAGCGGGA(SEQ IDNO.288)

[0600] EGFRvIIIDprobe0289:AAAGCTATTTGTTCAGACAGGAGTGGGGTGGGTCTGGGCAGAGCACTGCTCTAACTTGG

[0601] CCATGCCGTGGCAGCAGCTCCTTTAATGCCACTTTTTCCTGGCGCGCCCGCGGGGCCTGGA(SEQ IDNO.289)

[0602] EGFRvIIIDprobe0290:GCTCAGAAAGAGGGGAACGCTCCCTCGTCTCTCAACAGTTGCTCCAGACAGGTCAGCAA

[0603] ACATGGAATTCAGAATGTTCATTAAACACTGGCTGTGTCTTTTGTGTTCAAAAGCAAGACA(SEQ IDNO.290)

[0604] EGFRvIIIDprobe0291:CTCTCTCTGAACCATGGCCCCACAGAGAGTGCAGAATGTGTGAAACCTGCCGGGAAGGT

[0605] CTGGACCCCTTGCGGGGCAGTGGGCAGCACCGTGCCTCCGTTCACACCACTCACATGGCTG(SEQ IDNO.291)

[0606] EGFRvIIIDprobe0292:TGCCTCTGCTTCCTTCTGGCATGGCTGCTTCTTCCTCAGGTCTCAACCATCTCCCTCAGAT

[0607] GCTCTTTCCCATGTTTGTGGCTACAGGTCCCCGTGACCTGCAGAGGCAGAGCACTCACC(SEQ IDNO.292)

[0608] EGFRvIIIDprobe0293:AGCAGCCCAGCCTCGTTGCGCACCCATGTTTGCATTTGCAGGCCCTAGAACCACTCCAAG

[0609] CTCCGTGTGGCGAGATGCACCCTCCTGCCCTTCACTGGGGAGCTGCCCTCCTGTTCACAG(SEQ IDNO.293)

[0610] EGFRvIIIDprobe0294:CGGCACCTGAGTCACACATCTGGAGCCATCCTGGACTGCCTCATTTCCCCGATGGGGGGT

[0611] TTCCCTGACTTCATCCATCCTGTCTTTTGGGTCCCCATAATAACTGACATGGGTCGGCCC(SEQ IDNO.294)

[0612] EGFRvIIIDprobe0295:GTACCAGCCCCTGTGAGAAGGGCTTTAACTGCCTTCCCACCCCCTGCTCATCTTAGAGTC

[0613] TCTCTATAGTGCTGCTGAAAGAATCTCTAAATcagtggttctcaacctcagccgcacatt(SEQ IDNO.295)

[0614] EGFRvIIIDprobe0296:gagaatcacctgggacccttaaaaaaatcttaactcttggtccaagaattctattacaatcggtctgggatggggccctacaggtatttttttaaagct

[0615] ctccagttggtaatgcatagc(SEQ ID NO.296)

[0616] EGFRvIIIDprobe0297:tagagttgagtatcgctgTTCTAACGTGCAGATCTGGTCATGTTACCAGCCTTTTAGGTGGTCTTCTT

[0617] TGGCTTTCTCTATCTAAAGTTCAAAACCGAACATGTGCGCATTCAGTGCACC(SEQ ID NO.297)

[0618] EGFRvIIIDprobe0298:CATTTTCAACTGTGCATTAACACATTCAGCCCACCAGCAAGATTTATGAACCATTTTCTGC

[0619] TGTTGTATATAACATATCATATGCATAATGGCATAGGTTATTGTTTTCTTCAAAATATA(SEQ IDNO.298)

[0620] EGFRvIIIDprobe0299:TGAGATGTGAGTCCTTCTACGAACTGACTCACACTGATTGCCCAACTTCCTCTCTCGAGG

[0621] TCTCATCCTCTTTCCCTGCAGCCGTCTCCCTCTTGcacgcacacacacacacacacacca(SEQ IDNO.299)

[0622] EGFRvIIIDprobe0300:cacacacacacacaccacacacacCAGGGTCGATGCCATCTACCCTGGACTTCATCTTGAACTCCTTCG

[0623] AGTGTGAGTCATTACTCCTTTGTGCACCTCTGCTTTCTCTTCTCAAGATGT(SEQ ID NO.300)

[0624] EGFRvIIIDprobe0301:TCACCTGCTTGAGGTCAGTTCCTTGAGCGTCTTCCACTTGCCATGTTCACCACAGTGCTC

[0625] AACATGCCTGAATGCATGGATGGCGacttctcagatcctcagtctcctcatctgggtaat(SEQ IDNO.301)

[0626] EGFRvIIIDprobe0302:aaggcattgggttggcgggtccatctggtttcttccagctctgagagtgcatttgctctgtgattcATTCGTTCCACAACACTTCA

[0627] CCAATTAAAGAGAGGGTACAAAAGGTGAACATCC(SEQ ID NO.302)

[0628] EGFRvIIIDprobe0303:TTGGCTCCCAGCAGATGCTCCTCAAAACCTGAAAAATCAGATAGGTGAGGGAAGATTGA

[0629] ATGAAAGGCCTCTTATGATTCTGCAGCAATTTTGGTGGTTTAAGAACTCTATGGAAAAATC(SEQ IDNO.303)

[0630] EGFRvIIIDprobe0304:ATCAGTATTTCTGGAATTGAAGTAAAATGGATAGTGAGCCTCTGTGTATGTGAAGGCCCG

[0631] CATCTGGAACATGAAAGAACCTGTCTGATGTGTTCTAGTCAGGAAAGCAGGTAGCCAATA(SEQ IDNO.304)

[0632] EGFRvIIIDprobe0305:CTATTTATAGAATTTACAGAAACTGAAGATTTTGTTTCTACTGATTTTCAAAATAGTATTAT

[0633] GTCTGATTTTTTTCCTCAGAAATATACTTCCTGCTCTTCTCAACAAACTCATTTGAAA(SEQ IDNO.305)

[0634] EGFRvIIIDprobe0306:ATATGATTAGAACATGATAGAATTTTACTCATTTGCCAACTGCGGTTCCCATTTCACATATT

[0635] GTTAGAATTCTGCATGGTGGCTTTGCCCTTTAACCACTAACTGATAAATGATGTAGTT(SEQ IDNO.306)

[0636] EGFRvIIIDprobe0307:AGCTTTTAAATGTGTGGAAAAATATAATTTCAGGTTCAACCATAGGTCAGAAGTACACGT

[0637] GTTTTGTTAGTCTATTTGTCTCTCAGTCATCTCATGGAAAATTCTCAGCTTTTGGTATGG(SEQ IDNO.307)

[0638] EGFRvIIIDprobe0308:AAATAATTTTCTTGAAGGCAATATTTGTTGAGTGACTGACGGAATGAAAAACGCCAGTTG

[0639] CGTAAGTgtgaaaaagatctgggtgttttcattggatccaaattccacatgagccaacaa(SEQ IDNO.308)

[0640] EGFRvIIIDprobe0309:cagcgtggtgtggaggctggagcacattaataagaacagtgtcctaaattcaggaggtaatgctctgcccatgccctgtgcagctcagacggtgt

[0641] gtgcagtgcagtatgtaacccaggg(SEQ ID NO.309)

[0642] EGFRvIIIDprobe0310:cacatttcaggggcccacagggagctgcagcttgtaaggtggagtgcagccaacagagcagagagtcagaatccccgcagagtggttgaagg

[0643] cacaaggatgcgcagcaaggaagacaga(SEQ ID NO.310)

[0644] EGFRvIIIDprobe0311:cttataggtggtgcgactgccatcctctggtactgaaggtgctatcatggagggagggaagtagattgaccctcctggctccagagtacggaactc

[0645] agacaaacggtcagaagcttacag(SEQ ID NO.311)

[0646] EGFRvIIIDprobe0312:ggaggccaattttggatcaactttaagaagaattttttaaaagctagagcaatcctaaaatggaatttgctctttataaagttgcgaatgcctcaccctg

[0647] gaattgcttaagcaaagttg(SEQ ID NO.312)

[0648] EGFRvIIIDprobe0313:ggacgggcagttgtgagtaatCTCCTTTCCAATCCATACCCGCAATCACCAGAAACGTGGACTTCCCT

[0649] GACACTGAGCACCTCTTAATTAAGCATCTCATAAGTGAACAAAACCCAGCCC(SEQ ID NO.313)

[0650] EGFRvIIIDprobe0314:TTCAAAGAAGTCACTTTATTTATGTGTGGGTCTGCAGCTTGGATTTCTTGATAATGTTAAAT

[0651] AAAACTCCATCTACTCTTCCACAAACACTTCAAGAAACCTAAGACTTTTGGCCAGAGT(SEQ IDNO.314)

[0652] EGFRvIIIDprobe0315:AACACCGAGGTTTGAGAGAAAGGATATGTGTGTGAGAGGTGTGGTTTCATTAGAACATAT

[0653] TATTTGACTTCATGTTGAATCAACACTTTTGTGCAAAATGCAGTTTTACCAGCCTCTTTC(SEQ IDNO.315)

[0654] EGFRvIIIDprobe0316:CTTGTTTTGGTCACATAATTTAACTTAACATTCTCGGTACTTGATTTTCTAACATAAAATGG

[0655] GATTGAGAGGGGAATTTTGAAGTTCCCATGGTCTGTCCTCTACATTCTGACAGCTCAT(SEQ IDNO.316)

[0656] EGFRvIIIDprobe0317:TATCTCTGCGGTATTGTTCTCACATTTAAGTGAGGTTAGCGGAGGCAGAGGCCTCTCAGG

[0657] CCTGAAGATAGCCTCTGTTTTCAGGGAAATACTAGACTGTGAGATCTGTGACACTGAAGC(SEQ IDNO.317)

[0658] EGFRvIIIDprobe0318:ACTAAGTTCATCTCACAAAAGCAACGTGCTCTTTTTAAATGGTTGATCAAAGTTACTTTCA

[0659] AAAGGAAGTGTTAGTTTTTGTTATTAGCCGAAACAAGAGCTGCTTTAATGTAGTATATT(SEQ IDNO.318)

[0660] EGFRvIIIDprobe0319:AAGAGCTGCTTTAATGTAGTATATTTAAAATCATATCTCAATTAAGATGTTATTTCaaatactatttga

[0661] cccaccaatctcattactggatatacccaaaaggaatagaaatcattctat (SEQ ID NO.319)

[0662] EGFRvIIIDprobe0320:ccttagcaaacgaataagaaaagaaaacaaaataccgcatgttatcactttaataagtgggaggtaaatgatgaagaacacacaaggatacactggggc

[0663] ctacttgagggtagaggttgaaggg (SEQ ID NO.320)

[0664] EGFRvIIIDprobe0321:aacaaaataccgcatgttatcacttataagtgggaggtaaatgatgaagaacacaaggatacactggggcctacttgagggtagagggttgaaggg

[0665] agagaagcagaaaaaataactattg(SEQ ID NO.321)

[0666] EGFRvIIIDprobe0322:tgggaggtaaatgagagaacacaaggatacactggggcctacttgagggtagagggttgaagggagagaagcagaaaaaataactattggg

[0667] gtactaggcttagtaccagggtgacaaa (SEQ ID NO.322)

[0668] EGFRvIIIDprobe0323:aaaagaTGCTATGCAATAAAATTCTCAATTAAGAATTTAACTTGGTAAATGTTCATTTAATGAT

[0669] CTAAAAATATGTGTCTGGATGGCTCTAGCAAAAAAATAAATAATAAGTTTCTCAGA(SEQ IDNO.323)

[0670] EGFRvIIIDprobe0324:GATGGTAAGGCTGAAATAAATGGGGAAAAATCTGAATTGTAATCCTTTTTCTGTTGGACC

[0671] TGGTGTTGGGGTTTCACACTTGTGGGTGAATGTGGGCCTCCTGTGAGCACCAGCACAAAA(SEQ IDNO.324)

[0672] EGFRvIIIDprobe0325:GACTAAACTGAACAAAAGATTAAATGTCACCTCTAAAATTCTGTGCAACAAGACTTCCAG

[0673] CCACAGAATGTGCAACTCAGATTTCCAAGTAAAAACACACCAGGAAGCAGATCTTAGATC(SEQ IDNO.325)

[0674] EGFRvIIIDprobe0326:TCTGTTATCTCCTTGGCACCAGCTGGTATTCATCCTCAATGCTAGCTAGAGTTGAAATAAA

[0675] GAGTGAAAGAACTTTCTCTTTTATTACttaataaacttccttttttgagctgttttagg(SEQ IDNO.326)

[0676] EGFRvIIIDprobe0327:cttacagaaaaattgagtggcagtttcagggagttccagcacggcccctgtttctttctcatggtccctgcaggtttcccctattattaacgtctgtcatt

[0677] agcatggcacatttgttac(SEQ ID NO.327)

[0678] EGFRvIIIDprobe0328:aattaatgagccaatattgatacattattcactaaagcccacaggttgcgttaggggtcattctggtggtgtacgttcttcaggtctgacaaatctat

[0679] aatgacatgcattcaccatt(SEQ ID NO.328)

[0680] EGFRvIIIDprobe0329:actatatcacgcagagtcgtctcctggccctacaagtcccctcctcccacctgctcactcctccttccccccccaaactgtggcaaccattt

[0681] aacttttgactgAATGGATTTA(SEQ ID NO.329)

[0682] EGFRvIIIDprobe0330:TTCTTATTCTGCCTTATTGTATGTACACCATATTTTAATAAAATAATAGTCTATAGTA

[0683] GACTTCTGTAAATACTCAATGAATAAATACTTGCATGAATGCAGGAAAAATCAATCA(SEQ IDNO.330)

[0684] EGFRvIIIDprobe0331:GTCTTGCAGGATTTCTTATGCGTTACATCGTCCTTATAAAAGCAGTCATTCTCACCGAG

[0685] ATGTGCTGAGCAGATACTGGACATGTTCTGACCCAGATAAGGGCTGGGTGGAAGTAGGG(SEQ IDNO.331)

[0686] EGFRvIIIDprobe0332:CTGGAGACACAGAGACCCAGTGCCAACTTCCAGGACCTCGGAAGAACTGAAGGCAGAG

[0687] AGGTCCTCTCAGTGTGGACTGGGCCTCTGCTGGCAGCCCACCAGCGGGCACAGAGCTGATGTG(SEQ IDNO.332)

[0688] EGFRvIIIDprobe0333:TGTTATGCCACGTGGGGAAAACCTACAGACGATTCTGAGAAAGGCTCACAGGGACACCC

[0689] TCTGCCCCTAAAAGAACAATTTAACTCTAATTTATTTCTGTCACTCTGCATTTTCTGACCT(SEQ IDNO.333)

[0690] EGFRvIIIDprobe0334:TTCCCAAGTGTACAGTTTTATATGCATTTAACTGCCAAATTGTCATGTGAGATTATATGGTT

[0691] ATATTTCATTAATATATTCTAGTTTGTTCAGCTGTTCTTACTGGGTGAATTTGTGTGG(SEQ IDNO.334)

[0692] EGFRvIIIDprobe0335:TTTCCTGACATTTTTGTTTTTAGTAGTGCCTCAGTAGTTTTATACATAATTACGTTTCCCTTC

[0693] TGGATTATTTCCTTAGTATCTAGTTCAAGAAGTGAAATCGCTGGATTCTTGTGGTAA(SEQ IDNO.335)

[0694] EGFRvIIIDprobe0336:ATTTTTGAATTTCACAGTATAATGCTGATTTTCTCAAAGTCTCACATTCTAAGAAAGTATAA

[0695] TGAGGCAAAACAAACAACAAACATCTTAAGTTGATTTTTTCCTAGCATCTTTTCCTTC(SEQ IDNO.336)

[0696] EGFRvIIIDprobe0337:CATCTTTGCTTGTAGAATCTAGACTATTTCATGAACCCAAGATATAATCAGTATCCTTCTTC

[0697] AGTATGGCCAAAGTGAGTTTCTCATTATTTTACCTCCCCTTCAGGAAATGACTTTTCA(SEQ IDNO.337)

[0698] EGFRvIIIDprobe0338:TCTTGTGTTTTGGGAGCCATAGATGGTTCTGGGCAGGAAACTGGCTTTGGATAGACCCAG

[0699] CATGTAGATGGCTATTTGGCCTTGCTCCCAGTATAACGATGCAGTTCCCTGTGAAAGGGT(SEQ IDNO.338)

[0700] EGFRvIIIDprobe0339:ATGAGTAGGTTTTGGGGCTCTGGATACCGTGTGGCCTGAAGAGACAAGGGCTCAATGCC

[0701] AACTCTGCCTGTTTCCAACTGTGTAACCATGTGAGCGTCAAAAATCATGGACGTGCTCTGG(SEQ IDNO.339)

[0702] EGFRvIIIDprobe0340:TTAACACTGAGTGGGAGCTCAACAAATTATTATTTTTAATTGTTACTTGGACATGGCCAAG

[0703] TTGACTACACTTTATGTTCTGCTACCTGCCAGTCTGAAAGTGACGCCACAGAAGGTGAA(SEQ IDNO.340)

[0704] EGFRvIIIDprobe0341:CCGCATGTTGGGAGATGCTCCTCATCTGCTTAAATGAGGTGCAAACACAGCCCATGCGCC

[0705] TGCTCTTCATGACTGTATCTGTACCAGCAATATTTGTATTGGCAAATCACATGCCCCAGT(SEQ IDNO.341)

[0706] EGFRvIIIDprobe0342:GGGAACTACTTAAGGGGAATTCAATGGATTTCATTCCTTTTATGTAATTGGCCACTTAGTA

[0707] ATAGACGTGTAGGTCTCTTGTGTGGATAAGGATTCTGCCTTTTATGTAAGATATGTGTT(SEQ IDNO.342)

[0708] EGFRvIIIDprobe0343:GCAATTCAGCTTTCAGGTCCCAGCCCCGGGAAGGCTCCAGGCCTTCACAAACTGGCCCA

[0709] CCCACGAGAAGGAAAGCAATTGTCCAAATGTGGGTAGCTTTTCTTCCCACTGTTGTCAGCT(SEQ IDNO.343)

[0710] EGFRvIIIDprobe0344:GCTTCCAATTAGCCCCCATATACATAATCCCAGTTTGTGTCTGTATCAGTACAATTCTCCCA

[0711] TGTCAATGTGAATTTTAAGCCACAGAGGGAAAGGGGACAGAGAATATGCTTTCATTCA(SEQ IDNO.344)

[0712] EGFRvIIIDprobe0345:GCTCTCCTCGTCTCACACCTCTTGCCCTGCATGCATTTCTTTGCTCTGATTAAACGAGCAT

[0713] TTTATAAGCCACATTTGCTGTGTGAAAGGCAAAGTCTTCCCTCCCACGGATGACGGTCT(SEQ IDNO.345)

[0714] EGFRvIIIDprobe0346:gtgtgagagagagagagagagagagagaCTGTAAACATATATCTCTGTGAAACTTCATTTTCCATATGTG

[0715] AATTTTTGGAACCGAGACAAATGGAACTTAGCTAAAAGATGGGAAAGGTA(SEQ ID NO.346)

[0716] EGFRvIIIDprobe0347:ACCGAGACAAATGGAACTTAGCTAAAAGATGGGAAAGGTAGACTGACTCTGACTTAATC

[0717] TACTTAACCTACCAGGCAATTTATAACTTGATGGCCTAATTTTTGCAGCACCCAGAAGCAA(SEQ IDNO.347)

[0718] EGFRvIIIDprobe0348:GCCTGTTTCAGCACGGCAAAGGCTCAGCTGCTAAGTGGGCAGCATTGTTGGAGGTGAGC

[0719] AGCTTAGGCTGACTGTTCATCAAAGGACCAAGCGCTTGAGGTTCGCTCATCGCTGGAGGCC(SEQ IDNO.348)

[0720] EGFRvIIIDprobe0349:AGAGTGGGGAGGGCCATTTAACTGCTCAAGGCCATGGAACTCTACTGTCAGTTTCAGGG

[0721] AAATTTGGGACCCTGGAGCACAAACCAAAACTCCAATTAACCAGGAGAGGAACTCGATCCC(SEQ IDNO.349)

[0722] EGFRvIIIDprobe0350:CAGGAGATAAGTGAAGAGTAAGAAGTCTATCTTTAGAAACAAGAGATGTCCAAGGCTAG

[0723] AAAGATGGGGAAGGAGGGTGGAACTGTTCTGGAAGTGGGTCTCAATCTCAGCACCAGCAGC(SEQ IDNO.350)

[0724] EGFRvIIIDprobe0351:TCTCAAGACTTTCTAGAGAAGGAAACTTCATTTCTGAATTAAAATTAGTCTTCAATGACAT

[0725] GGCAGGGATTTCGGCACACTCTCTTGCGTCATAGGCCACTGTGTTGGAGGCAGGAGTGT(SEQ IDNO.351)

[0726] EGFRvIIIDprobe0352:TGGCTTTGGAGGCATAGAGATTAAAATTAGAGTAACACGTGAGCACTGAAAAGGTTAAA

[0727] CAGTAGAGACATGGAGGACTCCCGACCCCCATGTACCCCTTTCTTAACCCTTTAATTAAGA(SEQ IDNO.352)

[0728] EGFRvIIIDprobe0353:TCACAGCCCTAGAAATAGCTTGCAAAATAATTAACTACTGATCATTTATACCTTAGTGCTTC

[0729] TGTGAGCATGTTTTCTCTTTCATTGCTGCTCATCTGCATGGAAAAATGTGCATGGGTT(SEQ IDNO.353)

[0730] EGFRvIIIDprobe0354:TCTGAATATAACTCCATGGTGCTTGCTTCCATTATATTTGTGCCATTTGGATCATAACTGATA

[0731] AGCAACCAAAGAGTCCCATATTACTGCACGTTCCCATCGCTATTTTATGTGAAGGTG(SEQ IDNO.354)

[0732] EGFRvIIIDprobe0355:GTCCTGGGGGCTGTTCTGAATTCTCAGTTTCCTTTTTTCCCCTCCCCAGTTCTTTGAAAAT

[0733] ATCAGAAACGGACTTGTGGCATCTTTGAAAAGCTACTTAAAATGTGCTGCTGTGCTCTG(SEQ IDNO.355)

[0734] EGFRvIIIDprobe0356:AACTTGAAAATGTGCTTTTAATACAAAGTTTGTGCAGCCCTTGCTGCTCATACGAGATGA

[0735] ATCTtaccatgtggtggatgcccgtctcatgccaggcactgtgctctaagcccattggtt(SEQ IDNO.356)

[0736] EGFRvIIIDprobe0357:tatttcagtgcttgaaattggctttcgagagaggcaccacggttccctttttacaggagaggaaacaccagaggatcagagatggagagtctttctc

[0737] cacaaactcacagaccccaaagg(SEQ ID NO.357)

[0738] EGFRvIIIDprobe0358:caagctcagggttgtcagcttccaaagtctgcctgctccaggacctcatgttgcatctcCATTCTCTTCACTGAGGGTCAAAT

[0739] GGAAAGAACACATGGGGGTCAAGTTTCAGAAAATAAG(SEQ ID NO.358)

[0740] EGFRvIIIDprobe0359:AGAAATGAAGAAATATGTGCCCGGAAGCAAGAACGACCGACCTCATTAAACTGGCTCCC

[0741] TTCACCTCCTCTCACATCTTTTTCTGCCTTTTGGCCAAGTTTTCTCTCCCCCGCATTTCCT(SEQ IDNO.359)

[0742] EGFRvIIIDprobe0360:CCTTGATCTCGTTTGAATCCTCTTCCCTGGTGAAGTCATTTAGGTTCAGGCTCTTATTTTAC

[0743] TTTGGTCCATAATTTAGATCGAACCACATGTGCTGATGTGATTGAAACGATGTGGAAT(SEQ IDNO.360)

[0744] EGFRvIIIDprobe0361:TCTCTGGACAGAGATAGAATTATGGAGGGGTTAGTGTGTGTGTTTAAGATTAAAAGACCA

[0745] GGTGTATGGGAGGAAATATAATGAACAAAAAATAGTATTTTAAATGAATACTAAACTTGC(SEQ IDNO.361)

[0746] EGFRvIIIDprobe0362:ACTCATGGAAAAAGTTCTCTTCCCATGAGGTTCTCGCAAAGCATTTTACCATCAGCACAC

[0747] GCAGTTTTTCTCAGTTTTCTGAGATGGGGCCATCTTGAATCCAACAGACAACACACAGCA(SEQ IDNO.362)

[0748] EGFRvIIIDprobe0363:TCAGCCAGACTAACACAAAGGACGTCATGGGCATGGACGTAAATACTGGTGTCAACACT

[0749] AGGTCTGCACCTCGAGAGGAGTGGAGCAAAAGGATGGAGTGGCAGATGAAGGTATGCTGTT(SEQ IDNO.363)

[0750] EGFRvIIIDprobe0364:CAGAAAGGAGGCAGAAATGAAAGGAAGACCATCAGTGCGCTCCACAGCTTGAGGACCG

[0751] TCCTGGAGGGCAAATGCCAGCTGCTCACTTCTGAAAAGAAAAATTCCAGTGAAATGAGTACA(SEQ IDNO.364)

[0752] EGFRvIIIDprobe0365:GTCATTCTTAGGATTACTCACTTGATACTGTGTATGTCTCTTCTTGGCTTCTCATCTCCACA

[0753] CAAAACCCTCAGGTGGtaaaaatctaattaaaaaaattatataaaGTCTTGTAGATTT(SEQ IDNO.365)

[0754] EGFRvIIIDprobe0366:ATTAGCCTGAACATAATAGATTTTTTTTAAGCACGTTAAGTCTTCCATGGACTAAAAGAAA

[0755] ACTTGTAAACCTAAGAGAACCTCTATTTTTGATATACAAAATAATACATTTCCTTAAAC(SEQ IDNO.366)

[0756] EGFRvIIIDprobe0367:TATGATCTTGATACTAGAATTTTAATTAAAAAATACCTGCAGTTTATATGCAAAGTTATAGA

[0757] TTAATGCTTAAAAATAGGTTGTATGTAGTATCCACAGGTCATGTTTGACTGTCAAATA(SEQ IDNO.367)

[0758] EGFRvIIIDprobe0368:GATGTAATTTTAATTCATAATAATTGTGTCGTGTTCTTCCCCACTAGAAGCCAATTATGCAA

[0759] GCTTCACCATTCACACATGGAAAATAATTTAATGGAGTACTCATTGCAATTTCACTTA(SEQ IDNO.368)

[0760] EGFRvIIIDprobe0369:TCCAGAATTGGCTGTTGTTCTCAGAGCAGCTTGTGTTGCCTTGTTAAGGAGAATATGTTA

[0761] GTATCCAGACATCCAGAAAGGATCCTTTACTGTTTCAGAGTCCATTTTCCCCACTTTTGA(SEQ IDNO.369)

[0762] EGFRvIIIDprobe0370:AATACACACACAAACACCCATTCATgcaaaccaaacagagattgtaaagtgattccactgacatttatgcacttcttttttctct

[0763] ttggttcttcaaactctcagtcagtgcgcatttac(SEQ ID NO.370)

[0764] EGFRvIIIDprobe0371:tcttaatttagatacggtttaaacCTAATTAGAAACCAGAAGCTCTTGTATTTCCACAAAGGATTATGACA

[0765] GCCCCAAGAAAAGATAGTGAAACCATTATATAACAAGATAAAGGCTTCT(SEQ ID NO.371)

[0766] EGFRvIIIDprobe0372:TAACAATACAAGGATGGATTTTCTCATTGATCTTAGCCTTCTGAATTTTAGAAATTGCCATT

[0767] TCAAAGTCTAAAACAAAGGAAAATCAGGGAATAAAAGAATGGTAAGTAGACACAAACC(SEQ IDNO.372)

[0768] EGFRvIIIDprobe0373:TACTGGCTCCATCATTTCTGTTTTAGCAAATAACCTGCCACATATACCAATAGCCCAAGAG

[0769] ATGGGCATGTCCCTGCATTTCCTGGTCAAGGTGACAACACTGCGTCCTCCTGGAAGAGG(SEQ IDNO.373)

[0770] EGFRvIIIDprobe0374:TCTGCCACTCACCATACCACAAACCAAATATAATAAAATCAGAAGGCACACTATAGTGAA

[0771] TTTTTTAGAGGCATGTATTGAAAAGCATCTCAAAAAGCATTCTCGAAGCTTCCAGAAGTC(SEQ IDNO.374)

[0772] EGFRvIIIDprobe0375:AACTCAAGTTATCTGAAAAGTGACACTTTTGATGATTGCTCGCTTAATACTGGGAGAGCC

[0773] AGATGAAGATTCCTCCCCACTTCCTCAGATGTGCAACTCTGGAATTTCTTAGTGTTACTG(SEQ IDNO.375)

[0774] EGFRvIIIDprobe0376:GAGATTCCTGCTGCATTCTGGGCCTTTAATGCATAAACACTGAGATGTTCTAAGGAAATTA

[0775] CTCCCTAGGGAGGAGAGGGGTGGACGAGGAGTAAGCTTTGCTGGTGACTCATGCGCTGT(SEQ IDNO.376)

[0776] EGFRvIIIDprobe0377:GTGGAAACTCCCTGCACAAGTGAGCTGCGCAGGGTGAGTCTaaagggttaatgcactttcaaaagcctcta

[0777] atttgttattccagaagagtaatttactcactagaagtatctggGTGGC(SEQ ID NO.377)

[0778] EGFRvIIIDprobe0378:TACTAACACATTTGTGTCTTTAAAAAGATCAGTTTTATTTTAAGATTAAAAATATAAAGCA

[0779] AGAGCTGGAAAGTCACTAAAAACTGACAGCCAGTTTCCCATTTTCAAGAGTATTTATTA(SEQ IDNO.378)

[0780] EGFRvIIIDprobe0379:AAAGGTTCTGGTTGCAGAAGGAATAAGAAATGGCTTGAGATCATGACACAGTGAATCAT

[0781] GTTGTAAACATGTTAGCTATGGCTGTGAATTCAACCAGCGATGAGTTCAAGCGTCCCCAGA(SEQ IDNO.379)

[0782] EGFRvIIIDprobe0380:AGGTGTTGGGGGAATTAGGGACATGGCTGTGTTTCCCCAGAGAAAAGTGGCCATTTTACT

[0783] TTCCCTCTTCACTAACATGCTTTTGACATGCATGGCAGAGCTGAAGGCAAGGGGAAGGGG(SEQ IDNO.380)

[0784] EGFRvIIIDprobe0381:ACAACATAGTAAGTGACTAAGTGGCTTTTTTTTTTTTTTTTTTGCCAAGTGAAGCTGAGTC

[0785] ATATGGCCTCTGTCATTCCAAAACTATTCTCTACGGCTGCATTCCTTTCGCTCTTGCCT(SEQ IDNO.381)

[0786] EGFRvIIIDprobe0382:TCCTTTAGAACCCTGGAGAAGGCCTCCTGAAGCCTGGCCCTATTATGTATCCTGACAAAG

[0787] ATAAACTTTTCCAAAAAGCTGCATGTTGTTTCTAGCACAGTTTTTCCTCGCAGTGACTAC(SEQ IDNO.382)

[0788] EGFRvIIIDprobe0383:GTGATGAAAGTACCATGCAGAGGAGGTGTCTGACTGAGGCGTTCGTGGTGTGTGACAGA

[0789] GTCCCCTGCACAGGACAGCCGCACTCCCCTCTTGCGTCCTTTCCTCCCATGTTTGCAAAGC(SEQ IDNO.383)

[0790] EGFRvIIIDprobe0384:CTCTTTCCCTGTCAGCAGGGGGTGTTCTGGCAGTTGACATTTCTGAAAACTACAGCCTAC

[0791] ATTTTTAAAAAATCCAGTAAGTGAAAACTAAAAAATTAATACCGTGGTCATAATAGTGTG(SEQ IDNO.384)

[0792] EGFRvIIIDprobe0385:GCATTTGATAACTAATGAGGCACTGTCGTGCCAGCTATTATTTTCAGACATTTACAGTCCT

[0793] TTTTTAAATACAAAGAAATATTTGGTGTGAAATGTTCCCCGGGAGCTGGTGCAAGCAGA(SEQ IDNO.385)

[0794] EGFRvIIIDprobe0386:GGCGACAGGGCAAGGGAGCTTGGGTTGTAGCCTCGAATTCCTCCGGCCAGGGCTACCGT

[0795] CAGCCTGCGGCACACAAGTAAATCAAATATAAAACCAAAATTTCTGTAAGCAAATCAGTTT(SEQ IDNO.386)

[0796] EGFRvIIIDprobe0387:CTAACTCACTGTAACGAATTATCTTTCGCACATCACAGAGGCATCTCTTTTCACTGTCGAG

[0797] TTTGGTTTGCTTGGTTACAAAAAGGGCAGTTCAAAAGCTTTGGTTGCTATTGTGAAAGT(SEQ IDNO.387)

[0798] EGFRvIIIDprobe0388:CAGCTGAATTCCTTCCACCGTGCTGGGGTGGGGTGGGGTTCACGCAGGTTCTCTTTTGTC

[0799] ACCAGGGGTGCTGTGGATTCACAAGTAAGCAAGAGGCTCCTCAGGTCAAGCCTCTGGCTG(SEQ IDNO.388)

[0800] EGFRvIIIDprobe0389:CTCCCTGAGGTCAGCTGCCTAGCTTCTCCTCCTCTGAGATAGACGGGAACAAAGTCTTTG

[0801] ATGTGTGCATTTCTCAAGCTTGACAATGATACAGCTACATAAAAACCCATGATTTCATAT(SEQ IDNO.389)

[0802] EGFRvIIIDprobe0390:AGATATTCCAAAACGTAAAAGTAAACCATGCATCCACAGAGACATGGAATTACAGAACTG

[0803] GATGCTGAGCTGGTCACTTGGGAGGCAGGCGTCCTTGCCATTGGTTTATGCCTCAGCCCC(SEQ IDNO.390)

[0804] EGFRvIIIDprobe0391:ACCATGCAGTGGCTGGCCAGGTGACCTAGGCCAGTCCTGCATCCTCGGCTCCTCACCTGC

[0805] CTGGTGGGACAGTGACATCTCTCCTGCAGCACTGCTGTCAGGGTGAGGGAGGTAGGGCGC(SEQ IDNO.391)

[0806] EGFRvIIIDprobe0392:AGTTTCAGAAAACCATTGGGCTGCACCTGCGTGAGCACAGCTGCAGGAGCAAAAGTCA

[0807] GAAAGGTCAGCAAAGGATTTCAGGAGCAAAGGTCAGAAGAAACCCTCAAGGTGGTTGTGTCT(SEQ ID

[0808] NO.392)

[0809] EGFRvIIIDprobe0393:GCAGGAAAGTGCTGTCGTCTCCTGCAATGCTTTCAAGACTATTCAGAAGCACAGTGTGA

[0810] AGGGAGAGCCGGAGCCCATGGGGAAATGACTCCAGAGTGTTCCACGTGTTGGAAGGCATCT(SEQ IDNO.393)

[0811] EGFRvIIIDprobe0394:GTTGGAAAACGGACATTCAAGCAAATAGTTGCCTGCATAGACAACGCAGAATGACTGGG

[0812] AAAGCCCCAACAAGTTACCTACTGGTAAATGAGGTGAGAAGCTTAAAGTGAGAACCCCATT(SEQ IDNO.394)

[0813] EGFRvIIIDprobe0395:GCTGCCTCTTTTTCACTTTAAAAACATTTAAGttttgaattatggtaaaatacacgtaagatttactactgtaaccattttta

[0814] agtgtacggttcagtagtgttaagtatattcacattg(SEQ ID NO.395)

[0815] EGFRvIIIDprobe0396:ctaaggaaccaatctGCTACTTTTGTTTATTAATTTTTTCCTGAGGGGAAATAtttttaaattttaaaatatttaattga

[0816] caaataaaaattgtgtatattcaaggtgtagaacatgatt(SEQ ID NO.396)

[0817] EGFRvIIIDprobe0397:tcatatgcacgtacattgtatactcattaccacaatcaaagaaattaacacatccaacccacccatagttgccattgtgtgtgCGCGGATGTG

[0818] CGtgtatgtgtgtgtatgtgtgcacgt(SEQ ID NO.397)

[0819] EGFRvIIIDprobe0398:gtgcgcctgtgtgtgtctgtgtgtctctgtgtatacgtgtgtgtacatgtgtgtacgtgtgtgttcctgtgtatgtgtgtctgcgcacgtgtgtatgcatgt

[0820] atatgggtatgtgtgtac(SEQ ID NO.398)

[0821] EGFRvIIIDprobe0399:gtgtgtacgtgtgtgtgcatgtgtgtatatgtgtgTCTGTGGGCACAGgtgtgcctgtgtgtatgtgtatatgtgtatgtgtgtacatgtatgt

[0822] acgcgtgtgcatacgtgtgtgtgtgt(SEQ ID NO.399)

[0823] EGFRvIIIDprobe0400:gcacaggtgtgtatgtgtgtgcctgtgtgtgtgtgtgcatgtgtgGTGGGGACACTAAAAATCTCTCATCACCTTTTT

[0824] AGTCAAAAGAACAGTTGTTTTGGTTTGGCTCTTCTGTTTTAA(SEQ ID NO.400)

[0825] EGFRvIIIDprobe0401:AATATCAGAACAATAATAATTTCCCACAGACAAAATCCTCAATCCTCACCATCCTTCTATTT

[0826] CCTATATTCATCATAAACTTCATGCTTGATGTTGAAATTGTTTTCTGAAAATAGAGAA(SEQ IDNO.401)

[0827] EGFRvIIIDprobe0402:TACAAAGAGGAGATTTTAAAATGTCAGTGGCAGCCCCACACTCCTTTTTAATCTTATTTCC

[0828] TGATATCTTGAGTTTACTTGGACGTAGAGTTTTCCTTGACTATGGTTATTTCTGGTAGT(SEQ IDNO.402)

[0829] EGFRvIIIDprobe0403:AGCAGCTCCAGATTAGGCAATGGTTTTCTTCAGAGATAGCTTAGAGTGAGCCCCAGAACA

[0830] AGGTCAATGCGAAGATTGCTTGTGTCTGCGTGTCCAGGGCACAGTGATCCTCATCACTAG(SEQ IDNO.403)

[0831] EGFRvIIIDprobe0404:CCGGGGGGCTCCGTGAGGATCTGCTCCTGGTCGTTTCTGTTCTGTATCTTCTCTGCAGCCC

[0832] TTACTGAAGCCGTTACCAACTGGCACAATTCAATTCCTACTGTACCCATCATGCACAGA(SEQ IDNO.404)

[0833] EGFRvIIIDprobe0405:TGGCTGAAGTATTGAGAACGCTCCAGTGACCGGGAGGCAATAGTCTGTCCACATCTAAG

[0834] AACACACTTGGAATAACCTTAGAGAAGAGAGAGAGAGAGAGAATGCATGGTTAGTAGGTTA(SEQ IDNO.405)

[0835] EGFRvIIIDprobe0406:TCAAACTCCTATGACTTTTCACAGGAAAAGCCCTCATCCACACCAACTTTAGGAATGTGT

[0836] AGAAAGAAGGGTCAGGGACAGGGGTGAGTGGTGGGCAGAGCAGTTGGAGGGCACAGGGAA(SEQ IDNO.406)

[0837] EGFRvIIIDprobe0407:AAGGCATCTGGTCATGTATTTGGAGTAGGAGGTCTTGCTTTACTATTGAATTGCAGGGAC

[0838] ACTTTGGGAACAGTGTTCACTTCTTTTTGCAACCATTTCTTCAGAGAAAAGTCATGATAC(SEQ IDNO.407)

[0839] EGFRvIIIDprobe0408:TCAAGTCTTCTTACAAAGCAGTTTGAGGCTTTGAGTACCAGACTGATTACAGAGATGAGT

[0840] ATGAAGCATTATTGTAGTATTTTTAAGTGAAATTCACTAAATGCAAATAAACCTAGCAAA(SEQ IDNO.408)

[0841] EGFRvIIIDprobe0409:TGCTCTATGGTTAATTTTTTTCTAAAATTCAGATAATTAAGACAATTCATTCTCCTGAAACT

[0842] GCTGTTCATGTAAAAAGGAATTTTATCGAGGTGGCCCTTGAGTGCCAAACAGCCTGTC(SEQ IDNO.409)

[0843] EGFRvIIIDprobe0410:CTCAGCTGCAAAATGAGTCGTTGATGATCCTCCAGCAAGGGATACTTTTTAGCTCGTGTG

[0844] GTGATTGCTGCACACGGGATATGTGCAGCAAGTATCTGCTGAGCTAATAATAAACAGCCT(SEQ IDNO.410)

[0845] EGFRvIIIDprobe0411:CAGACAGAAAGACAGTGGGCACAAGGTCATGCTTAAAAAGACCCCTTGTTCTACTGCAT

[0846] CCCAGCTCCCCACCATGGGGCCTCACAGGCCCTGGTGACCAAGCACATCAGACCTGGTTCT(SEQ IDNO.411)

[0847] EGFRvIIIDprobe0412:TGCTCAGTCCTGGGAGCCACAGAACCCAGCACGTACTTTACCCCCAAGACCAGACTCCA

[0848] GCTTGGCTTTTGTCCTCCTCTCCAGGATTGGTGACCTCCTAGGTCGTGAAGCTGTGATGAG(SEQ IDNO.412)

[0849] EGFRvIIIDprobe0413:CAAAGACACACTCCTCTCCATTCTCCCAACTTCAGGTCCCTTTGACAGTGTCAGCAGGCA

[0850] TTTAAATAGCAGACCACCCACAGCAGGGCTGGTAGATGCAGTGAACTCAGGAAGATGCCT(SEQ IDNO.413)

[0851] EGFRvIIIDprobe0414:GCATAGACTCTAGTGTTAAAGACAGAATCCTTACAAGGAACCCCCATAGTTACCTAACTG

[0852] CTGTCTCCAGTGGTCATAGAAGTGTGATAACCCACTAATCATCATTCTCTGTCTCTCTGT(SEQ IDNO.414)

[0853] EGFRvIIIDprobe0415:CTTTCTCATACACACTTACACACACATACACACAACCTtgttgcttaattttcagagagtctactttcagaaaagc

[0854] cttcaggaatacatcatgtacaaaactgagaaattacctgAAGT(SEQ ID NO.415)

[0855] EGFRvIIIDprobe0416:ATCTTTAAATTTAGTAAAAAGTTGCATTGTTTTTTGAACATCACACTTGAAAAGTACATGA

[0856] ATACAAACATACTTAGGAAAAAAAGCTTTAATTAATTTAAAAAGGAGAACAATGCTATA(SEQ IDNO.416)

[0857] EGFRvIIIDprobe0417:TGCTGTATCCCACCTTTCTCTGAATGTTACATTTTCTCCCCTATCCCAGGCTGCATCTAAGA

[0858] AAACTCAGAGGGAATATGCTATCTATCTTTTCCGAGCAATGAAAGCTCTGGGTTTTTT(SEQ IDNO.417)

[0859] EGFRvIIIDprobe0418:CCTTGCTTTTCAGGGCACAATACTTCTCTTTCTTCCTGGTTAGACAGGATAAGTTCTGAGT

[0860] CCCCTGGTATCATCAGCTTACTTCTTCTCTGTTAAATATTCACAAAAAATCACTAACTT(SEQ IDNO.418)

[0861] EGFRvIIIDprobe0419:TCATGCCTCAGCAAACCTCCACTGCCTAAAATATAGTGAGGTCATTCATCTTCGGACAAAT

[0862] TGCCCCAACTACGGTGGGAAAAGAACCAATGTGTTGGACTATTTATCTAATTTTTGTTT(SEQ IDNO.419)

[0863] EGFRvIIIDprobe0420:AGTTCGGGGATACAAATAAATGCATAGATACATACAAACATGCGTACATAATAGCAGCAGC

[0864] AGCCTGTGAAACATTGACAAGACCTGGAGTTGGAAGAGGACTTTGCCATCCTCCAGTCC(SEQ IDNO.420)

[0865] EGFRvIIIDprobe0421:AACAGTTGCCTGTCACAGATTAGACGACTGGGATGTGCGCAGGCGATTATTTGCAAACGG

[0866] CCCTGAGTCCCCCAGTTTATGTCTTAATTCGCAGCCAGGGCTGATTGTAGAAGCAAATTT(SEQ IDNO.421)

[0867] EGFRvIIIDprobe0422:GCAAACATGTGCAAGAAGAAATCACACATCCTAGAGCTTGGATTTCCTCGTTTCTTGCTA

[0868] TTTCTATCCGTAGACAGAACCATTGCTGAGCTGTTAAATTTGTCTCCTTCCCCTATACCA(SEQ IDNO.422)

[0869] EGFRvIIIDprobe0423:GTCTTGAAAAAGGAAAGGAAGTGGAGCAAAGAAAAAGAAATTAATAAAGCCGGCAGAT

[0870] CCTAGGAGAATCTTATTTAATCCAAGCTTTGTAAAGTTTTGCTTTATTCCATGGCAACATGG(SEQ IDNO.423)

[0871] EGFRvIIIDprobe0424:GTATACACATCCCACCGGCTGTTTCAGTGGCTCAGAGCAGGTAAGGCCTGTGCCAAACG

[0872] CCGCTAGCAGGAGGAACAACGTGGAGACAGCCCCAGAGGTGGAACGTTGGCCCTTCTGTGG(SEQ IDNO.424)

[0873] EGFRvIIIDprobe0425:CTCCGGTGTCTCAGGACCTCCCTAAAGCCCAGCCCTGACACTGAGCAAGTTTCCACCAC

[0874] TGTTAGGAAGAAGTAGAAAGGAATTTGGAGGGTTGGTGTTACTGTTCAAGAGCTGGAAGGC(SEQ IDNO.425)

[0875] EGFRvIIIDprobe0426:TTCTGCCCCCATTCCCATTCCATTAATTGCGTGAGGTAGAGAACTCATAGAAGATAGGAAC

[0876] ACATATGCTGATTTCCAAAATTGCCTTTGTATATTTTCACGTGAAGACTTTAGGGGCAA(SEQ IDNO.426)

[0877] EGFRvIIIDprobe0427:AAGAAAAGAAGCAAGCATTTTGAATATGTGTTTCAATTTGCCTTCTGTTATATAAAATTGT

[0878] ATTTTGCCTATTCTTTTTTCATTATTCGGAACCTTCAAGAAATAAATTAAGTTCTCTCA(SEQ IDNO.427)

[0879] EGFRvIIIDprobe0428:AAAATGTGTTTTTTGAAAAGAGGACTAAAACAGATGGCCTGGCTGTGTTAAACACAGGG

[0880] ACCAGACCAGCACCCACCTCTCCACCTGCCCTGCCTTCACTGGCAGAATTGTGATCCATCA(SEQ IDNO.428)

[0881] EGFRvIIIDprobe0429:TGTTCTCTGTTCAATGTCATCATCCCTTTCAGAGCATGGGTCTCTTCCTTTCTAGGCAGTC

[0882] TTACCAGGATGCATGGGTGTGCCTGCGTAGGCACACGCACAGCTCCCAAGGACTCTAAA(SEQ IDNO.429)

[0883] EGFRvIIIDprobe0430:AAAAGATATTTTTCTGCTTATATACTAATAATATGTTAGAGATTTATGTTTCAAATTAGTACA

[0884] GAATCACATGGTTCTCTCCAAATTATATTTGAGAGAGAAAGAATAGAACAAAATTTA(SEQ IDNO.430)

[0885] EGFRvIIIDprobe0431:TTTTACAAAAATACTCAGTACATTTAGGGCATATACAAAGATGTTCCAGAATGTAGCTTAT

[0886] CTCTTTAAAGACAATTAACACAGTTTCTGGGCAAGGCAAGGCAAAATATTCAGTAACTT(SEQ IDNO.431)

[0887] EGFRvIIIDprobe0432:AGCAACACCAACAGAAGACAGCCAATATTGCAGCACATTTTTCTCTTGGATTGGGTCAGA

[0888] GAGTACTGCAGAGAAAATGGAGTAGAGAGACCTGAAATACTTTCGCACACACTGTGGTCA(SEQ IDNO.432)

[0889] EGFRvIIIDprobe0433:GTGCAGCGTCCACTGTGTGCCACAGTAATACTAGAAACTCCCTGGTTAGGCCTTGGAATC

[0890] CAGCTCTCATTTCGTATGTGACCTGCAGGGAAGTAAGTTAAATGCACACGTTTTATCAAG(SEQ IDNO.433)

[0891] EGFRvIIIDprobe0434:TTCAAATGCAAACTTAATTTTAAATGTATGCAACATCAGTTTAAGCGTTGTAGCTATTACTA

[0892] GCAATTGTACCTATTACTAGTCTGTACTCTGCACAACTTTGGAGTATACTGCCTACTC(SEQ IDNO.434)

[0893] EGFRvIIIDprobe0435:AAGGTGGATTTTAGAGCTCTATTTGTGGCATTATATCACGGACaaaagcacgttcatcagagtcagaggaa

[0894] tgtggtgcaaatcccagctgtcccacttaccagctgtgggacttgagta(SEQ ID NO.435)

[0895] EGFRvIIIDprobe0436:agctcctgaagcagctgcacctgcattttctggtgggcaccatggagctgtcagcagtgctttcctcagagggctgcgggctggatgaggtttgct

[0896] ggtgcatgtgaagtgTCAATCATT(SEQ ID NO.436)

[0897] EGFRvIIIDprobe0437:GCTCTCATGAGTGGTGATGCTGATGCCGTTCCCTTTTTTAGGGAAGTGATTTTCCCTTACA

[0898] AAGTTACCAACAGTTTCATGTTGGCCCATTTTTCTATTAATTGTTTCCACTAATAGGAC(SEQ IDNO.437)

[0899] EGFRvIIIDprobe0438:CAACAGTGGTAGTCCCATCATTTTATTACTGCTTGTCGTAGCACAAGCAGTTGCTTCATTG

[0900] TGTTTAGATAAATATTGACGGCTGCTTTTAACAGTCTGCTGTTTTGTCTCCTTTTGAGG(SEQ IDNO.438)

[0901] EGFRvIIIDprobe0439:TCCTTAAAGTAATCCTTAAAAAGATAGTGCAGATGGAAAGATGTCTGGAGTCAGTGAACC

[0902] TGCCTTCTTTCCTGTGTGCTTGTCAGTTTCTAAAATGCCATACACAAAGGACTTTCATGA(SEQ IDNO.439)

[0903] EGFRvIIIDprobe0440:TTTCTTTTTAGGTACATGATTACAGTTCAATTCACTTCACTGTCTGGAAAATTTCCTTATAA

[0904] TCAGGATGAAATTTCTCATGTTAGCCTTTCACATTTCACTACTTTTAGATAAGGAATT(SEQ IDNO.440)

[0905] EGFRvIIIDprobe0441:CTCAGGCTTTGCTATATCTGACTGCTCTTGGAGGCTGAGCTTTTGGCTAACTACCTGACTA

[0906] CTTTGTCGTTTCTCTTCCCTTGGAATGAAGCAAATATCTAACTTCTCACTCATTGTTTC(SEQ IDNO.441)

[0907] EGFRvIIIDprobe0442:TGCTATTTTACCATTTAGTCATCTGTGATTTTTCTAAATACTGAAAGACTTCCCTCAATTCA

[0908] AACTATGTGCCGGATCAAGGAAAGGGCAGTTGGATATTGCAGACAGCATAGTGCAATT(SEQ IDNO.442)

[0909] EGFRvIIIDprobe0443:GTGAAGAGTGTctgcttaccagccacgctgccttgcacaagttatcaagcctctcaacccacttcctcaatctgtaaaataggtatgagtg

[0910] taggaccttcccaggggatttttttgtga(SEQ ID NO.443)

[0911] EGFRvIIIDprobe0444:ctatagaatgaTTCTCAGAAGACTTTCAGGCAGTATGTGGGTGAGGCACATGCTGGAAAGGCTT

[0912] CTGCAGGTGCAGTGATCAATGCTTTTCTCAGTGTGTACATCCCATAATACAGACAC(SEQ IDNO.444)

[0913] EGFRvIIIDprobe0445:GTTACCAGAAACTCCCTAGCCAGGACTTTGATTGCAGCTCACATTTTGTATATGGCCCATA

[0914] GGGAAATGAAGTGTGTATTTTTTATAAAGTTCAAGTGTTAACTTAATTTGGAATTTACT(SEQ IDNO.445)

[0915] EGFRvIIIDprobe0446:ATCAAATCTCAGTTGTTATGGGCATTTATAGCTATTAATACTTCGTCCCATGTGTCCCATGA

[0916] GGAAACCAAGGAACAGAAATTAAAGTTCTTTCTGGAGTCCCCTGAATCTCGTTCCTGT(SEQ IDNO.446)

[0917] EGFRvIIIDprobe0447:TCTTTTGCACCCTGTTAATTACATAGAGACATTCACAGCTCTTCTGACCTTATCAGCGTTA

[0918] AGGAAAACAGAAAACCAGCGTGCTATTTGTTCTGTCCCTTAGTCAAGCCTTCTCAACAT(SEQ IDNO.447)

[0919] EGFRvIIIDprobe0448:ATATTTTTCTTCCAAGATTTTGCATGTGCACAGGGATGCCTATCCTCTACAAGAAACACAT

[0920] TTTAGGCAAATTATAATTAAAATGCTGTTTACATCTCTTCACCTTTAGAATTTAAAGAA(SEQ IDNO.448)

[0921] EGFRvIIIDprobe0449:TGATCATTTCTTAGATTGCATCTCAGACACACCCTTCCCCTAGTCTGGAGAGGGCGAGGC

[0922] CCATGGGTACTGCAAACAGCCTGACGTTGTCAGGGGCGGTCTCAACGGCTCATTCACCAC(SEQ IDNO.449)

[0923] EGFRvIIIDprobe0450:ATCTGCCTCGCGAAGGCTAAGCCATGTGCTGTTACCCCTGCTGCGCTCTGGCTCATTCTAA

[0924] GGTACACGCTATTAACCTTGTGAGAAAACAAAGAGGCCAGCCCCACCCTTCCTGCTCAC(SEQ IDNO.450)

[0925] EGFRvIIIDprobe0451:TCTGAGTCACGGTGAAAATGTTTCAGGATCTCGGGTTCGACCATGAGTCCTGTCCAGGTC

[0926] CAGGAGGAAATTCGGAAGGACCACATGTTCACTCTGAGATCCCACTTTCATTTCCCTCCT(SEQ IDNO.451)

[0927] EGFRvIIIDprobe0452:GGTTGAGCAGCATTAATACTCTGGCTAGATTTAAATTCTGGCTTTCTCCAGTTAGAACTGA

[0928] AAGTTATGACAATGTAATCAAAATAGAATGTGGGTTTACAGCTGGCCCCCTGGCCTGGT(SEQ IDNO.452)

[0929] EGFRvIIIDprobe0453:TTGTGAACATAAAACAGAAACAGAAAGTGTAAGTGGTGACATCATATTCTCTCATTCAAT

[0930] GTGAAAGGCCACCGAAGTCTTTCCAGAATTATTTTTGAGaataatatgaatttttaaaaa(SEQ IDNO.453)

[0931] EGFRvIIIDprobe0454:atacctaattattttaaatatCGTCTTGCTTGCTCCCCAAATACCTACTGTTTTCAACTTGGATATACGAC

[0932] ATGATTAAAGAATATCTAATATTTGGGAATGCATACTTTAACCTTATAA(SEQ ID NO.454)

[0933] EGFRvIIIDprobe0455:ACTACCACTGTAAATAGACAGACTCATTAAAGTGAAAGGACATTTTAAATCAATTAGTAA

[0934] GCAAATCAATTAGGTGGCAAAGACAAGATTATTTTTCCTTATGGTAGTTGAAGAATAATG(SEQ IDNO.455)

[0935] EGFRvIIIDprobe0456:CTTAACCTGTCATTCTAATTACCAAGCACGGTGTTCTCTTTGGAAGATCATTTCAACAAAA

[0936] CATTATTTTCATCCAGAATTTGAACCTTGAGATTGCATGGTATTTTAGAAATCTATTTT(SEQ IDNO.456)

[0937] EGFRvIIIDprobe0457:AGAAATCTTTGGCAAAGGTTACTATTAAAACAATCACATTCATGGAAAATCAGTATAAGA

[0938] GCAACTAAAATAACTCACAATACCAGTAAAATCACTTTGTCATCTTCTTAAGACTTTTAA(SEQ IDNO.457)

[0939] EGFRvIIIDprobe0458:AGAGCATTTGTAAGTAACTGAATAGAAGGCCAAAGGGTGTGTAGGTAGCCCAGACCATC

[0940] AGTGGGCAGCCAGGGCCAGGGCAGGGGCCACGGTTGCAGCCTGCATTCTTCTAAAGGGCAG(SEQ IDNO.458)

[0941] EGFRvIIIDprobe0459:AGCAAATTAAAGTTGAAGCAGGAGCTAAAAAAAAAAAAAAAAATGTTTCAAAGAATTC

[0942] CACCAACCAGAGGATACTACCTAGGACAGTTTGGGCCTAACTTATCTGTGAAGGCCTCCAGC(SEQ IDNO.459)

[0943] EGFRvIIIDprobe0460:TTCCTCCACACCGGTGGCCACTTTTCATTCACTCTGAACCCTTCTTTGTATGGAGGTCATT

[0944] TTATTAATTGAGCTGTGACCAACATGACAGAATTTCCTGTTTTAGGGCTTTTATAATAT(SEQ IDNO.460)

[0945] EGFRvIIIDprobe0461:AGATAGTTTATATCTAATTTCAGAATATATTCACTGGGGAATGGACTTAGCAACCACTACC

[0946] ACAACAATGCAACAATGTGTTTTGGAACAAATTTACCAATCTGAATTTCCCCCTAGATT(SEQ IDNO.461)

[0947] EGFRvIIIDprobe0462:AGGTCACAGGAACATTGCAGCTGATGTACAGCTATGTTCCTCCTGAAACTTGGAGACACA

[0948] TCCTCTTGAGCTGGGTTATAATGGGCCACCCAAAGCTCGAGTTCCTGTAATGGATACACT(SEQ IDNO.462)

[0949] EGFRvIIIDprobe0463:CAGGCAGCAGAACCTACCACCGTAGTGAGGACAGCACCCAGAGCCCTCAGAGGCCATC

[0950] ACAAGTGCACCACAGCTGCCTTCTCTGGCACGCTCAGAGCTACACAGTGTACTCTGGGATTG(SEQ IDNO.463)

[0951] EGFRvIIIDprobe0464:GAACTCTTTATTTTTTTTTCAGTTGATTTGTAAATAAGATTGCACAAAAATCCATGCACATC

[0952] AACTCTCCAAATCAGAATTTGCTGAGCTAAAAAGAGCATTAAATTAGATGGGCTGGCT(SEQ IDNO.464)

[0953] EGFRvIIIDprobe0465:TTCAAGGGGTGGGGGTGCAATAGTGGAACTCTGCACAACAGTTCTTTACAAAGAGACAA

[0954] GCAAGCACATCGCGTGGAAATTTCCATTCAACTGGAAATGTCCAAGCCTGTTTACCTCAAT(SEQ IDNO.465)

[0955] EGFRvIIIDprobe0466:TAATTGTCCTTGTTCACTTGTCCAGCCTAGCAATTGTCCATTAGTAATTTGTTATAAATGAG

[0956] ACATTTGGTATTAAAGCATCTCTTTGGGATACTGGTATGGTTTATTATAACATTCTGT(SEQ IDNO.466)

[0957] EGFRvIIIDprobe0467:TAGTAGTGTTGTACAAGCTTGAGATGTATTAATACGAAATCCAAGCTGCATGAGGGCTTTA

[0958] TTTTTCAAGCCTACACCTTGCTGAAATTCTGAATTAAAATATGATTCTCAGTACAAATG(SEQ IDNO.467)

[0959] EGFRvIIIDprobe0468:AATAAATCAACAGAAATGGTAACGCATGTCAAATATTCTTAAAACCCAAGAAAGCCTTGT

[0960] AACTTCCTTCAATCTAATGGGAAATGCAGGCAAATACAAGACTGATGTCCTTGAGTTTTA(SEQ IDNO.468)

[0961] EGFRvIIIDprobe0469:TTATCAAGACTCAAGGGCACCAGTAAAATCTAGTTTCATTGGTTGGAAAAAAAATCCTGA

[0962] TAAGCACTGTTAGGCATATTAACTTTAATGATTACAATTTTTAGGACACTCTGTGGCCTA(SEQ IDNO.469)

[0963] EGFRvIIIDprobe0470:GACTTAGAAACACAACTAATGTCCAGAAAAAGATTCCTCTTTTTATTCCATCATCTGATAG

[0964] GCCTATTTTTACACATACACACCAACCAAAAGTAGCCAAGCAAACAAAACAACATACTC(SEQ IDNO.470)

[0965] EGFRvIIIDprobe0471:ACACCCCTTCGCCTATTATCATCTAGGTGATTTTCAATGCTCATTGCAATGAAACCTACTTA

[0966] TTGTGCATGGCACCCACCCCCACTGAGGAATACTGTAGTTTCTTTCCCTTTGAACTTC(SEQ IDNO.471)

[0967] EGFRvIIIDprobe0472:ATTAGTAGAGCACATGGTTCATTCACTCCTGAAGAGTTCTTCGTATGTCAGAATATATATAC

[0968] TACAACATAATTTCCATCAGAGCTCTGACCACCCGCTTATCTATTTTCATAATGCCTG(SEQ IDNO.472)

[0969] EGFRvIIIDprobe0473:CCACTCCATCATTAGCTGTTGTCATGTAGGCTATCAATAAATATATGACAAATAAAACAGTT

[0970] AGGGAATGAGGGAAATTGACTAGCAGCCAAAGACCTAAGCCATCCTCTGCTTGGACAT(SEQ IDNO.473)

[0971] EGFRvIIIDprobe0474:TAGAAAACTGAGTTCACTACAGTCATAAGATACACAAAGGCAGAATGTAAGCCATACAA

[0972] AAATCCATGTCAATCCCAATATGTGAGTACAACTATTGAACACCATGTACTAATGGATGAG(SEQ IDNO.474)

[0973] EGFRvIIIDprobe0475:TTGGTAAATCATTCAATGTCTTCATGAGGTCAATTACAGATTATTATTTAGACCCCAAAGAT

[0974] TCCAAAGATGGTATTTCGGTCAGATCTTCATCCTTTGTAAGCCTAGCAGAAAATATGG(SEQ IDNO.475)

[0975] EGFRvIIIDprobe0476:CAGTTTTATTGACTACTATTCTTTGCTGGGTGTGGTATTTTTAAACTGAGACATCAGTGTG

[0976] CCTAGCACAGGGCCTCAAGCACACAGAAAAATTCCTTGATAATAATTAAATAAAATTTC(SEQ IDNO.476)

[0977] EGFRvIIIDprobe0477:AGCAAAAAATATCATCTTAAGGCTGTGAAATTATCTTCCTGTGTGGCTAAAATAGTGAATA

[0978] AAATTCAGCGCAATATAAATCATAGTACAATTTCATCACTAAATTTTCTGATCTTGATC(SEQ IDNO.477)

[0979] EGFRvIIIDprobe0478:TTGTCATTTTACATTGGAAGTAAAAATGTGTCCTCCTTTTTTTCTCTGACAGTGAAAAGTG

[0980] TGTGTGTGTTGTGTGCCCTTTTGCACACCCTGCCTCACACTTGCTGGTCTAATTCCTTC(SEQ IDNO.478)

[0981] EGFRvIIIDprobe0479:CAGCATGATTATGATATAATTAAATGACAGAAATGTTTACTTCCAAGTGGAACTAAGCCAG

[0982] GGTAACTCAGGGTAGGGCAGCTGCTTGCACCGAAAGACCAAGACTGCTAGAGAACTAGG(SEQ IDNO.479)

[0983] EGFRvIIIDprobe0480:AAACAGGCGGTGCAAGAACTCCAGGCTCTCATGGAAGAGCGGGAGGCTTCTATGGGGCT

[0984] GCAGAAACTCTTTGGTGCTTGGGGAAAAAATGGGTTAAATGCTCTTAAAAAAGAAACCTGG(SEQ IDNO.480)

[0985] EGFRvIIIDprobe0481:GAGAGGTAGTTTCCAGATGCAGGCCCGTCTTTTCTTTTAAACAGAGGCAGCTCCGAAGA

[0986] GCTGGACATTGAACCCTGAGCAGGAACTGGAGGCCGTCAGCGCAGCTTTGTTTGGCGAGCG(SEQ IDNO.481)

[0987] EGFRvIIIDprobe0482:GAGCTTTGCAAGGGTGTAATGCTGCACCAGGGAGACGCTATCTGCAGGGACCGGTGACG

[0988] CCGTGGGTGTGGAGGGGGAGGCAGTGGCTGGCCCTCTTGGGGTAAGGTACGCCCAGGAACA(SEQ IDNO.482)

[0989] EGFRvIIIDprobe0483:GTTTAGAATAACGTGCGCGAGTCAAAGGGAAGAAGAAGCTCCTGCAGACCTTCTGGGCA

[0990] CTGTGCAGGGTTTGCTCCTGTCCACCGTGCCGTGTTCCTGTCCTGGGGTATTTGGGTGTGT(SEQ IDNO.483)

[0991] EGFRvIIIDprobe0484:GGCGTGTGGGGAGGGGAGAAGGAGCAAGGCGGCAGGGAGGGGATGAGGACCACCCTG

[0992] TCCATGGGACAGGCCCTGGGCCCCGCACACACCCCAAGCCCCGCGTCCCGCGTCCTCACTGTC(SEQID

[0993] NO.484)

[0994] EGFRvIIIDprobe0485:CTGGGACACCCCCCACCCCACCCCACCGCCACAGCCCAGAGCGGTGCCAGGAAGCCGC

[0995] CTCGACGCAGCCGTATCTTGAGGCTCCAGCCCCATCCCCAGGGTACCACGCCACGTAGAGAC(SEQ IDNO.485)

[0996] EGFRvIIIDprobe0486:ACTATTTTTCACTTCGTGTTTGTCACTCCTAAAGCATGTGTGCTAGCTGCACCAACCCTGG

[0997] GATGCCTCGGTGCATAGGGTTTATGTGCGTCCTCCTCCTTCCCTCTGAGCTGGTCCCCC(SEQ IDNO.486)

[0998] EGFRvIIIDprobe0487:GTGGGGAACTGCTGCCCAGACTGACCTGCGTCCTTCCGCACGTGCAGGAAAATGTCCAC

[0999] GTGCACTTGTCAGGGTGGGGGCCACACGGGCACCACCACTGATCATCTGTGGGATCGAGTT(SEQ IDNO.487)

[1000] EGFRvIIIDprobe0488:ACTGCCCATGCAGATCCCACGTGCAGGGCCCAGTCGCTTTGGTGAGAGAGTGGACGCTG

[1001] TGGTGACTCCACGGTCTGTGGCTGTGCTCAGGAGGACAGAGAGGGGACATCCTGAGATGGT(SEQ IDNO.488)

[1002] EGFRvIIIDprobe0489:TTGGGCAGCCCGCGGATCCTGTGCATGTCCCCAGAGCGTCCACTTTCTCCATGGAGCAGT

[1003] GGAGTGGCGTTGCTGAGACAGAAAGTTCAGGTTCTCCACTCCCCATGCAGCCCCCACTCC(SEQ IDNO.489)

[1004] EGFRvIIIDprobe0490:CCTGTCTCCGGCCAGGCACGCGTCTGGGGTGGAGACTCCCGGTGCCCGGGGCCCTCCAG

[1005] ACCTCTTTCCCCACCCCAGGGAGCAGGCGGGTACTTCTATTCCGTTTGGCTTCAGAAGGGA(SEQ IDNO.490)

[1006] EGFRvIIIDprobe0491:AAAGAGAACGTAAGTTCAGGGAGTTCTCGTCCATTCCTCTCCCGTGGGCCGGGCAGGCA

[1007] GCAGGGACAGCCTTCAGGAGCCAGGAGGGGCTCGAGCTGCGAGGCCCTGGAATGAGGCAGG(SEQ IDNO.491)

[1008] EGFRvIIIDprobe0492:CATGGGCTGAGGCTGGAGGGAAAGCCCCGCTAAGGCTGGGCGGGGGCGGGAAAACTTA

[1009] CCACCAGGGGACTCGAGATGGGGAAGGAAAGGTCAGAAGAGGAGAGGCCAGGCACGGGGTGT(SEQ ID

[1010] NO.492)

[1011] EGFRvIIIDprobe0493:GGGCGGCCTGCAGAGCTGGAGCAGGTGCTCCGCCCAGAGCCAGGCATGCACACTCAGA

[1012] GTAGGTGGCCTGTGCAGCGGGGAAGAGGGGCGGGTCGGCGTGCTGCTGAAGATGCAGGAGCT(SEQ ID

[1013] NO.493)

[1014] EGFRvIIIDprobe0494:GCGGCCTGCTCTGTGCGTGCTGAAGGTGTGGTGAGAAGCACTTACAAAAAGAAATGGAC

[1015] TGTGTTAGGATTGCACATTTTACTTTGTTTCTCCCAAATACGTGTTCTTTGAATTTTTTTC(SEQ IDNO.494)

[1016] EGFRvIIIDprobe0495:CTTCCAGGGCCAGGACTGGAGTGATGGTTGAGACAggcacgcactgggtcttgtctgcatttacattttgagatttt

[1017] gttcagcatggattttATGGCgtttttttgtttgtttgtttgt(SEQ ID NO.495)

[1018] EGFRvIIIDprobe0496:tcgtttTCAAAATACTGCACGGtttatcgtgaagacagggtcctttgctgccgtcttaagttttgggcccaagaacgtgccccacccta

[1019] ggcccgggcctgCTGGCTTCATAGCTCTCAT(SEQ ID NO.496)

[1020] EGFRvIIIDprobe0497:CATTCCCACGGAACCTTAAGACCTGAGGACAGAAAGGAAGGAAACAAGCCCAGTAGTC

[1021] CGTGAAAATCCAGGGTCCCGCCACTCCAGGTGTCTGCAGCAGAGCTGAACACACGTAGGCTC(SEQ IDNO.497)

[1022] EGFRvIIIDprobe0498:TTGCCAGGAGGGGCATTTGTATGTGCtgagcattccttatattctcaatatgacgcctttgaaagatctgtggtttgcaaatatt

[1023] tactctcagtccataacttatctttccaacctctt(SEQ ID NO.498)

[1024] EGFRvIIIDprobe0499:accaggctcttttgctgaataaaagttttaaattttgaagtctaatatatttttaatttttttattttatggatcatactttttgtgtcaggtttgagaagtctgca

[1025] ccaaagtatgtcc(SEQ ID NO.499)

[1026] EGFRvIIIDprobe0500:tgtggttttcccttaggtcatcttcaacaagtttcatagtattttgtttagatgtaaatctgtggcccattttgagttagtttttgcacaagagttgaggtcaa

[1027] ggttctttttttgcct(SEQ ID NO.500)

[1028] EGFRvIIIDprobe0501:gtgatgttcagtggctctggcaccatttgttgaaaaCATGATAGCCAATGTCAAGACTTAATAGTTATAATAATC

[1029] AGGAGCTTTTGTTTCTTTTTGTTTTGTTTTTAGTAACTGCCAGTC(SEQ ID NO.501)

[1030] EGFRvIIIDprobe0502:ACTGCCAGTCACTGCTtgtggtatacatacacaatggaatactattcagtcttaaaaaaaaaaaaagaaggaaatcctgtcatttgcat

[1031] acctggaggacattatgttaagtgaaataag(SEQ ID NO.502)

[1032] EGFRvIIIDprobe0503:aaagaaaaacattgcatgatctcactccttcatggaatctaaaaaattgtattcagagaagcagagagtggaatggtggttaccaggggctgggaa

[1033] ggtgtgagcttggggagatttggt(SEQ ID NO.503)

[1034] EGFRvIIIDprobe0504:gaaaggacatagaatctcagttagacaggaggaataagttaaagagatctattgcacatcatggtaactgtagttagtgacaatgtattgtatacatg

[1035] aaaattgctaagagagtagatt(SEQ ID NO.504)

[1036] EGFRvIIIDprobe0505:agagtagattttaagtgttctcaccacaccaaaaaaaggtatgtgcagtaatacagtcattaattagcttgatgtagccattccacaatggatacatat

[1037] atcaaaacatcatgttgtata(SEQ ID NO.505)

[1038] EGFRvIIIDprobe0506:atactgtctctttatgtaaatttaaaaaTAAGATAAAATAAATGTTATTCACTTGTCGTGGATGTGGTGGGGA

[1039] CAGGTGTGGGATAGCCCTCCCTGTACAACTAGGACCCAGGGGTGATC(SEQ ID NO.506)

[1040] EGFRvIIIDprobe0507:TAGTGACACTAGCCATTTATCAGGACGTATGGGTGCCAGTCAGGATGATAAAGCTTCCTTT

[1041] TGGCCACTATACTACTTAGAAATGCCCTGCAAAAGGTGCACATCAAAGATTGAAAGCTC(SEQ IDNO.507)

[1042] EGFRvIIIDprobe0508:AATCCTGGATTTTAAGTGCTTCAAAAGTGCACTTAATTGCCACATTTTTGTCAAACATTTT

[1043] CCCAGGTAGTATTTTTCCTCATGTAAAACAACAGCAATTTAATTTGAACAGAAAGCATT(SEQ IDNO.508)

[1044] EGFRvIIIDprobe0509:TTGAAACATACTTTTGGCAGGGTTCCTTGCAGATCAGAATGGAAATGATTAACAGGGCAA

[1045] TTATCAATCATGGACTTTTGGCGGCAGAAGGAACTGTATTGTTTGGTACAGTCTGGGCCA(SEQ IDNO.509)

[1046] EGFRvIIIDprobe0510:GGGCCACACACCGTAACGGAGATACTCTATTCTGTGGACGGTTGGAGGGGGCTGTGCTG

[1047] AGCAGGGTAACTGCATCTTTTCCTAGACTGTTCACACTGCTGCCACGAAGGAGTCTTGTTT(SEQ IDNO.510)

[1048] EGFRvIIIDprobe0511:AGACTGGACCTGGCTTTCTTCTTCGCAATGAGTGTTGCAGACTCCCGACAAAGGCCAGG

[1049] TGGTAAAGTGTGGTGTCTGTGAGCGAGAGCCTGAGATGCCTGAGCTGACCTGTCCTCAGCC(SEQ IDNO.511)

[1050] EGFRvIIIDprobe0512:ACCTGCCATCGTGCAGAGGTGAGAGCAGCCCCTGAATTCTGCCCCTCGGTCTCTCCATAG

[1051] CTAAAGCAAAACCATCCTTCCGTGCTCCCAGGACAAGCAGGCTATTACCAAATCACCCAC(SEQ IDNO.512)

[1052] EGFRvIIIDprobe0513:TAACCCTGGGCGAGGAGGGGCCATCACTGCACAATTCATCAGTGTCTGTGACAGGAAGA

[1053] GATTGTTTTAGACTGGTTTTTTTTTTTTTATTTGCAAGCTTTTTTCTCTCTCCAAAACGTG(SEQ IDNO.513)

[1054] EGFRvIIIDprobe0514:CTGTCAGTGTGTTCTAATTTACTCTGTAAGGAATTCTGGAGCTAATCATAGGCTCACAAAA

[1055] AGCAGCACAGGAAAGTTTCCCAGATAACATCTATTTcagtggctttcaaacatttttga(SEQ IDNO.514)

[1056] EGFRvIIIDprobe0515:ccttaccaaagtaagaaatacattttaatatcatggcacacatacagctgtatctaaactttcataatactgcctttacgatatcactctgatattgtctatt

[1057] cttttctgtttattttt(SEQ ID NO.515)

[1058] EGFRvIIIDprobe0516:ctttttgttccttgttatgctggttgtgacccactccagtgatttcacaatgcaggctgggtggtgtcccacagtttgaaaTCCCAATCTAGG

[1059] GCCTTCCTCTCACTGTACAAAGTAGGT(SEQ ID NO.516)

[1060] EGFRvIIIDprobe0517:AACTGGGGACATTAGTGGATCAGTGATCAAACCAAAGTTATTTGATCTTACCAAGTGATAT

[1061] CAGGATGAGAAAGCTGTTAGAGTGTCAGATATGTGAAGGAACTTGGGTCATTCCTGATA(SEQ IDNO.517)

[1062] EGFRvIIIDprobe0518:CCTCAAAGAGAAAAAAGGTAGTCCTTGAACACCTCCTACTTGTAAAGGATGCACAATCC

[1063] TACATGCCCCTCCCTTTCCTTTCCTCCCCTCTGTACCCCACCCCTGCCCACATTTTCTTCA(SEQ IDNO.518)

[1064] EGFRvIIIDprobe0519:TAAGCAGCTTTGGTGTTTTGGCTTGTTTGTTTCCCTTGTCTCCTACCTGTGACTTTATAGCC

[1065] TTTTGGAGACTCACAGCAATAGTTGTATTTAAACTCAGTGGGTGGCATCCAAGGCTAA(SEQ IDNO.519)

[1066] EGFRvIIIDprobe0520:AAAGGAGATTGCCTAGACACAAAACCACCCAAGGGAGAAAGCAGGACAGCATCTTACTA

[1067] TGATTGTTTCTTGTTTCTTCCTGTCTCATAAGGATTATTACCCAGGGTTTTCATTTTTTTC(SEQ IDNO.520)

[1068] EGFRvIIIDprobe0521:ATTTCATGGTTCATTTTCGCTCCAGTGTAGACATACAATAGACCACTCGTCCCTGTGGCTC

[1069] CGGGCAGCAGCCTCATCTGAGACCCTCCTGAGACATCTCGTGCAGGGCAGCCGTAGTGT(SEQ IDNO.521)

[1070] EGFRvIIIDprobe0522:GTGGCttccccagggctgctctaacagatcaccatccttgccatggcttaagaagctgcagatttatttgcttacagctctggaagccagaagtc

[1071] caaaatcaaggtgtcagtagagtct(SEQ ID NO.522)

[1072] EGFRvIIIDprobe0523:ctctctctgaaacctgctgaggatgatgcccctggcctctccccagcctctggtgttcccagcagcccttggcattccttgccttgtagatgcaaaac

[1073] tccgatctccacctctatcctc(SEQ ID NO.523)

[1074] EGFRvIIIDprobe0524:acagtgagttctcctgcatgtctgtctctgtgccttcacattcctctctgtgtgtctgtgtttccatctccttatgaggacacccatcactgaatcagggc

[1075] ccactctataccagtaaga(SEQ ID NO.524)

[1076] EGFRvIIIDprobe0525:cctcatttcaactccattacatcttcaaaaaccccattctcaaataaggttacttcacaagtgctggaggttaggacttgaacataCCTTATTGA

[1077] ACAATCCAACTGATGACACATAGTA(SEQ ID NO.525)

[1078] EGFRvIIIDprobe0526:ATTTATGCACTCGTTCTTGGAGACGTTGACTTTATTTAGTAGCATTAACCATGGCAATGTC

[1079] ACCAGCATCGCTGACAGCCTGAAGCATATGATCTCCAGAATGTATTTCAATCATCATGT(SEQ IDNO.526)

[1080] EGFRvIIIDprobe0527:TCACTTCCTTGGTATTCTTTAGACAATAACTCAGCCTTGAACTCCAGTAAAGGGTTTCCCT

[1081] GGGATTTTCTTCTTGACTCACTCCACTGTGGCCTCCCTCATCCAGGACTGTAACAGACG(SEQ IDNO.527)

[1082] EGFRvIIIDprobe0528:CCTGACGTCAGTGGTCTAGACCTCTCTGCTGAATGTCATCTTTGGTGAATGTCTTATGAGA

[1083] AAACACATGGTTGGTCACTCTTAGAAGGGCATGAAAGCCTGTCTGCAGTATAACCAAAA(SEQ IDNO.528)

[1084] EGFRvIIIDprobe0529:CAGGCACATGGCGAGGCACACTGTGCGCATGTGTGTACAATTAATATCATGGTTTTAAATT

[1085] ATTTTCAGGCCAAGGGGAGATCTTTGCTGCATCTACTGAAGAAAGCGAATCTTTTTCTT(SEQ IDNO.529)

[1086] EGFRvIIIDprobe0530:CCTGAAAAAAAATGGCTACTTATTAGTCGAATTTGTGTTTTAAAAATATGTGAACTAATAT

[1087] AATGCAGACATGCATTAATGTTTAAATATACTGGAAGTTTTTGGTAAAATGAAACCCAT(SEQ IDNO.530)

[1088] EGFRvIIIDprobe0531:TGTCTCTGTTGATTACTTTGATGAGTCAAGAAGTAACATCCTGGGAATGATTGGCCAGTTT

[1089] AAATGAGTGCCTCAGGTTTTTGGAATACAAGAAATCAAGAGGAAGGGATTAGAACATAT(SEQ IDNO.531)

[1090] EGFRvIIIDprobe0532:AGGTTAGCAAGATTGGGATCCTAAAATACAGACCCAAATGAATGGAACAAAATCAGGGA

[1091] ATTTATTAATAACAGGGTCAAGGCCAAATCAGTAACAAATATCCTGAGTGGAAGAAAGGTG(SEQ IDNO.532)

[1092] EGFRvIIIDprobe0533:GTTTAACAAATGCCCCTATGAAAGATAGAGATTGGCTTACCATGATGAGATGTAAGCCCA

[1093] AGTTATGAGGTTGGCACacaaaaccacaaatgtcatagcttaaaacaacacacacttctt(SEQ IDNO.533)

[1094] EGFRvIIIDprobe0534:atctctgtttctgtgggtcagggtctgggttctcagggactcacaaagtatgttttcatctggagctccaggtcctcttccaggctcataagggttcttg

[1095] gcagaattcagtttcttgag(SEQ ID NO.534)

[1096] EGFRvIIIDprobe0535:gctgtaggactgaggtcctggctcctagaggccaccctctccataagcagttcttagcatggccgcctgcttctccaggcccagtgggaaagcat

[1097] gtgcctccaggagggctcagtccat(SEQ ID NO.535)

[1098] EGFRvIIIDprobe0536:tcttcatggcttttacctggttaagtcaggcccactcaggataacttcattttgtattaaatcaaaaccagctgatttgggatgttaattacatctgcacaa

[1099] cttcaactttgccatata(SEQ ID NO.536)

[1100] EGFRvIIIDprobe0537:acctaaccatgggactgatatttatcatGCATTTGGGTCAAGTTGCATTAAGAGATATAATAAAGCTGGACA

[1101] AGCTTCTGTTGATTAGAAGAGTTCAGTTACAAGGCTACACTTGGGAGG(SEQ ID NO.537)

[1102] EGFRvIIIDprobe0538:AATGTTTACAAACTGGAATGGTCAGAGGATGGGGAAGACACTTGAGAAAAGTCAAGTG

[1103] ACggatgaaggcaaatgtggatatttatctgggagaaaactaagaggagttataatagctgt(SEQ IDNO.538)

[1104] EGFRvIIIDprobe0539:cttcaaatatttaaagggcttttattaggaagaGGAATTTGGCATATTGGATTTTGCCTTCAGAGAAGTGGAGT

[1105] CCTGAGATGCTCTTAGCCATTCATTCCAGCCTCCAGGGCTCACCTG(SEQ ID NO.539)

[1106] EGFRvIIIDprobe0540:CTGTCTTCTGTCCAGGTTCTCGGTAGCAGGGCAGTACAGCCCCATCCGTGATCTTCCATA

[1107] GTCAGGCATATTGTCACACTCAGTGAGCGGAGAGTCAACCGGGAGGAAGGCACAGTTTCT(SEQ IDNO.540)

[1108] EGFRvIIIDprobe0541:CTGGAATGACCTACGGAATGGTACGCTCAAATGCAAATTCTCCTTCCCTTCCCCAGTCCTT

[1109] GTCCTTCAGATGGTAATTTAGGAGCTGAAGGTCAGGGCACCAGCAGCCTTTGGAAGCCT(SEQ IDNO.541)

[1110] EGFRvIIIDprobe0542:ACAGGACAACAGTCAGCCTGGCTAGAAAAAAAAACAATGTCACAGGCATGTTGTGTTTA

[1111] ATCACATGAAGGATATTTGCATTGTTTTCCAACTGATGCCAGCAGACACATTGTCAGTGGT(SEQ IDNO.542)

[1112] EGFRvIIIDprobe0543:ATCATGCCTGGGGTATCAGAGTTGACATTGGGTTGCCCCTTCTCTGAGGCATTCATGTAAA

[1113] TCCTTTTAAGTTTATAAAACCTCCATGTGGCTCCTGCATGCTTCATCATTTGCATGTGT(SEQ IDNO.543)

[1114] EGFRvIIIDprobe0544:CTCTTTTTCCAGGGGAGGCAGCATGGGGAGCAGGATGCTGGTGGGCTCCAGGTGCAGAG

[1115] AGCAGGGTGGGCGTCAGACCCCAGGTCCACTGTGCACGCCCTCTTGTAGAGCCCGTTCCGT(SEQ IDNO.544)

[1116] EGFRvIIIDprobe0545:TGTCCATGAGATGAGGAGTGTTCTTATCTCTAAAGTATTATCATGAAAACCTAACAATGTA

[1117] GAAAGACTAAAGCACATGGGTGGTGCTTCATAAATAGTATTTCTCCCACTTTCTGAAAA(SEQ IDNO.545)

[1118] EGFRvIIIDprobe0546:CTCCTGCTGAAGTAACTGCACAAGAATCCTTGAACATTTAGAATTCTGGTTTTAGCCATAC

[1119] CATAAAGTCAGTAGTGCGTGGTGGAATTCTGCTAACGAAAATTGCGAAGGATCAAGGCA(SEQ IDNO.546)

[1120] EGFRvIIIDprobe0547:GAGTACAGAGCTGGTGTGTAGCGGGTACCTTCTGTCTGCTGGCACTAGGTATTTTACACA

[1121] TTAAATCAGCTCGTTCTCACATCAGCTCTTTTAAAAATAAGGAAATGAGGAGCCACAGTG(SEQ IDNO.547)

[1122] EGFRvIIIDprobe0548:GCCCAACTGATGCAGTGGCAGAAGTAGAATTTGAGCTTGTGCAGATGTGCCTCCGTGTTT

[1123] TGTCTCCTGAGCATGCTGCCCCAAGTTTGACAATACCAAGATTTGTACTGGAACATTCCC(SEQ IDNO.548)

[1124] EGFRvIIIDprobe0549:TCCCATCCCCACCCCCTAGAAGCCCCTCTTCCTCCCTTAGATTTGACACATAGTTTGAAAC

[1125] CACTATTAACTACCTTATGagagccactgtttgtgaagtgctgactatgtgccaggtcc(SEQ IDNO.549)

[1126] EGFRvIIIDprobe0550:cgtgccgtgcaatttttgtgaattatctcgtgtctacagtgcctcacaatttctctgctcaatacctccatgttactgccgaggaaagggaagctcaga

[1127] gagagtaagtaatttgctcga(SEQ ID NO.550)

[1128] EGFRvIIIDprobe0551:gttaaagagctggccaggacagccaggggcttgcaccccggAGCCTTCATCCACTACACTGTCAGCTGGTATCT

[1129] CAACCAGCCATTACAGGCTGTAAAAAAATTATATAAGATAGTCTAT(SEQ ID NO.551)

[1130] EGFRvIIIDprobe0552:GGTAATGCAGAAAAGTGAGGTTATTTTGCTCCCTTTCCCTTTGAAGAAAAAAGCCCTGGA

[1131] AAGACATATCACTTGAGTATGGGAAAAAATGAAGCTGTGGCTTTTCTGTGAGTCAATTCT(SEQ IDNO.552)

[1132] EGFRvIIIDprobe0553:TTCCTGGCAGCTTCTTGGAATAAGACCAAGTATAGCAGCAGAGTTTTCTGTTTTAATTTGA

[1133] GCTGCAGGGTGACTTTTTTTCTTCTATGCTTTCATCTCTCTGTGGCTTCTTTTGCCTCG(SEQ IDNO.553)

[1134] EGFRvIIIDprobe0554:TTAATTTCATGCCCTGCCCAGGCGGGCTACTGTGCTGCCCAGTCACCCGGGTCTGGGGCG

[1135] GCCACCGCTGGCCAGCAGGCAGGCCCTCCAGAGGCAGAGGTGGCCACGCTTAGGTCGCTC(SEQ IDNO.554)

[1136] EGFRvIIIDprobe0555:CCGCTGTGGAGGCGGCACACTTGGGTGGCAGCACAGCTGTGATGTGGCGGCAGCTGGC

[1137] AGCCCCATGGGAAAGATGTGTGAAGTGTGGGGTTTGACGACCCATGGGAGAACAGACTTTCT(SEQ IDNO.555)

[1138] EGFRvIIIDprobe0556:TCCTCTTCTTGTTTTCCCTTCAAAGCCGTGAGTCAACCTCAAATTCTCTGTCTTTTTTCTC

[1139] CACCCCCTCGTGCCTCTCTCCCTCACGCTCTGCATCTCTCATTGCAAGCTTGCATTTTT(SEQ IDNO.556)

[1140] EGFRvIIIDprobe0557:TTGCACACAACACTATCTTAATATTTCTCTTTTCTGCAGGCAGGAAATGAGAAGTCATTTT

[1141] TCAGGGTCATTCAGGAAGTCATCCAGAGTTATAATGGCCCATTATCTACTGGTCAGAGT(SEQ IDNO.557)

[1142] EGFRvIIIDprobe0558:TTACTTAGGCTTTCACTACTTCCACTGCCCACTTGAAACAGGGAAAAATATTTTCCCCCCG

[1143] CGCTGTGAGTGTGCTATTTAGAGCTGACCACAAGCGGGGGGAAGAGAGGATGGCTCGGA(SEQ IDNO.558)

[1144] EGFRvIIIDprobe0559:TGCTGCATTTCCACTGAGAACACAAGGCTGGCAAAGCTTGTCTGCTGCCCAGCAAGCAC

[1145] TTCAGGCTCACACCATTTTAGGTTCACTTTAAGTAGTTTCTCAATTGTTaaaaaaaaaaca(SEQ IDNO.559)

[1146] EGFRvIIIDprobe0560:aaaaaaaaaaaaCCTGTACTCTGAGGATATGCTTATAATCCCATAGCTAACCCAGAATTTCTTAGA

[1147] GAACTGatcaacatcagcagtggcacttactgaaaatgcacattctcaggccct(SEQ ID NO.560)

[1148] EGFRvIIIDprobe0561:gcgtagggcctactgagttagaatattagagagcaggtctcagaaaCATTCTATCCGGCAGTCTTATTCTATGCACCC

[1149] GAAGGGATAAGAGCCATGCTTTCATGAAACATGGGTTGTGTG(SEQ ID NO.561)

[1150] EGFRvIIIDprobe0562:TAAAATGTTTAAAAGGTATGGCAAAATGTGTTTGATTGGCACCAAGGATTTCTGGTTCCTC

[1151] CTAGAATCATTAATCAAACTTTGAAGGAGAAATAAGAGAGTCGGCATTTTCTTGCACAT(SEQ IDNO.562)

[1152] EGFRvIIIDprobe0563:TCTTTGTGATGTTGTGATGAGTTGGAAACTTCCCGATTGGGTTTATTAGAGCATGAACACC

[1153] CAGGCACCCAGCTTCTAGCCAGCCCTGTCAGGCAGAGTCTCCTCGAAGATGTGGAAAGG(SEQ IDNO.563)

[1154] EGFRvIIIDprobe0564:ACTGACCAACAGCTGAGGCCTACAGGAACCTGAGCAGGCAAGGGGAGAGGCACCCCGG

[1155] AACCAGGAGCAATGGCCTTCCCACCCTCCCTCGTCCTCTCCTCTTCTCCTTTTGGAGTTGCA(SEQ IDNO.564)

[1156] EGFRvIIIDprobe0565:GGCCACAGAAAGGAAGTGACATGAGTCACTTTGGGCCTTCTTAATTCCTTCATCAAAGGC

[1157] AGCACAGGTGTGTATGTGTGTTGGTGGCTAATTGAGGTAGGCCCACAGAGGAGATAACAG(SEQ IDNO.565)

[1158] EGFRvIIIDprobe0566:cacacacacacacacacacacacaTTCTCACTTCTTTGGCATCTACCACACCTGCCCCAGTGCCCATTT

[1159] CTCTCCCACCTGAATAAAAAGCCCCCACAAAGCCTGAGGTACATGGAAAGG(SEQ ID NO.566)

[1160] EGFRvIIIDprobe0567:TCTCCCACCTGAATAAAAAGCCCCCACAAAGCCTGAGGTACATGGAAAGGAGCAGTGGT

[1161] CTGGCTCCCAGGAGTGTGAGAAGCAGCCATGTTTTCAGAGGCTGTATTCCACTTGGACTTG(SEQ IDNO.567)

[1162] EGFRvIIIDprobe0568:GCCCTACGCTGAAGGTAGGAGCGGATGGGGGAGGCCCCCTTCGCACAAAGAGCCCCATG

[1163] AAAGAGTGCACAGTCCAGTCTATAAAACAGACGCAGAAAATGTGTGTAGGACTTCTTCCTG(SEQ IDNO.568)

[1164] EGFRvIIIDprobe0569:AAAAAGAGCGTGGTGCGTCCAGTACCTCCATGTTCATGGAACTTCCCAGTCTGCAGTTTA

[1165] CCCTTTTGTGCAACTCCCTTTTGGTAAAGCCCTGGTCACACTTCTGGTTGTTCAGATTAT(SEQ IDNO.569)

[1166] EGFRvIIIDprobe0570:ACAGGGATAATTCCAGAGTGATTTTAAAGTCAACTGCCAGGCATCCGCACTTGCAAATTA

[1167] GATGCCTGGCACATGCTTGTGTTAAGGTAATAATTCATTACAATACAAATTACAGGGGAG(SEQ IDNO.570)

[1168] EGFRvIIIDprobe0571:TTCCTCTGGGCATGCGACCTTTCCCGTCATTTGGCTTTCCCTGTGATTATCAGGGGAGCTT

[1169] CCATCGTGCTGCTAATGGGACCTTAACCATGTGTCAACCCATGGCTGTAATGCTGACAC(SEQ IDNO.571)

[1170] EGFRvIIIDprobe0572:TGTTTTCTTTCTGGAATGAAAGGCCTTCGCAATTGAAACCAAAATGTTATCCAACTCAGT

[1171] CCTGTCCCTTTGACGATGAAAACATCAAGTTCTGGAGACTGGCCATCCAGCCTCCCTGCC(SEQ IDNO.572)

[1172] EGFRvIIIDprobe0573:TCATCTCCCACGCCCTCCATCATTTTTTGTCTCTACTTACTTATTTATTTGGCTGTATTTTAC

[1173] GTACATCATGCAAAAATATTCCTCTTTGTAAAAAGTATAATGATTTCAGGAAATTAG(SEQ IDNO.573)

[1174] EGFRvIIIDprobe0574:AGGGTAAAAAGCAAGAACCATGCTTTCACTCCACTGTCAAGAGTTGTGGAAGAATCCTT

[1175] CCAGCATTTTTTCTGTGTATTTTACATACATACAAATATATGTACAAATAAAGGTCGATCA(SEQ IDNO.574)

[1176] EGFRvIIIDprobe0575:TTTAGGTTTTGTTTATATTTTTGTATATATGAGCTTATGTCATTCATACATATTGTTTTGCCTC

[1177] TTGCTTTTTTTTAACTTAATTTTACTTTGCTTGAGAGCTTTTTGAACTGAAGTACG(SEQ IDNO.575)

[1178] EGFRvIIIDprobe0576:TGTAAGTCAGCCTATGCATGTAATGGCTCCCTCATCTTCTGTGAGGCTGTCACTAAAAAG

[1179] GGGATTTAGCTTGTTCTGGGCTTTGCAGCCCGTACACTGGGCACTGTTCATACGTACTTC(SEQ IDNO.576)

[1180] EGFRvIIIDprobe0577:TCTGTGCACGCAAAGGAGGGCTTGCTAGGGAGGCCTGGCAGAGGGTGCCATTCAAATAG

[1181] GATTTTCAATGGAGGAATTTTTAAATTTTCAGTTATTTGAATAAGTTTTAATATATATCCA(SEQ IDNO.577)

[1182] EGFRvIIIDprobe0578:GAACCCcaaatcatcaagtttgttttcttccacatctgtccttccatttctgaactattttaaggccagtcatgtctcatccaagaaatcccatccttt

[1183] cacacaacactatctccgttt(SEQ ID NO.578)

[1184] EGFRvIIIDprobe0579:catggttatgaatctctaaaagcatgatttttaaaacataatcacaatgctgtcatcgaacttaaaaattagccataaatctcttatgttacccaacaacc

[1185] agcctactgacacatctcc(SEQ ID NO.579)

[1186] EGFRvIIIDprobe0580:agttgtctcaaaaatgtgttttccattgtggtttgtctgaaacatgatccaaaAGTCAGACCCACCTCTCACCTTTCCCTAA

[1187] CCTGCCGGAGCCCATGTTTCTTTCCAGCCAGGCTTGGA(SEQ ID NO.580)

[1188] EGFRvIIIDprobe0581:GACCACCACACGGGATTTGCTTCTTGGGGCCTCCCTCTAACCAGCTATGCAGGATGCCCT

[1189] CTTTCCTGTCAATACAAGCTGCTCAAAGGACTCATTCAGTTCAAATTCACCTATGTGAGC(SEQ IDNO.581)

[1190] EGFRvIIIDprobe0582:CCAGCTATGCAGGATGCCCTCTTTCCTGTCAATACAAGCTGCTCAAAGGACTCATTCAGT

[1191] TCAAATTCACCTATGTGAGCCTAGGTGATGCtacttatttatttatttatttatttattt(SEQ IDNO.582)

[1192] EGFRvIIIDprobe0583:atccacccacctcggcttcccaaagtgctTCATGTTTTCAGGAGCTGTACGTGCATTTTTAGTTTTGATGAC

[1193] CAGGTCCTTTTTCTGTTTTTTAAAGAACTTCAAATGATCTCCAGGGTA(SEQ ID NO.583)

[1194] EGFRvIIIDprobe0584:TTTTCTGTTTTTTAAAGAACTTCAAATGATCTCCAGGGTACACAGCGCTTGTGTGCTGATG

[1195] AAAAAGCTGGCAGTACAAAGGCCACCAGCCAAGGTCACACAGCCAAAAAGCCCCTGACC(SEQ IDNO.584)

[1196] EGFRvIIIDprobe0585:TCGGGCCCCTTCCCAGACCCTGGGTCTTTTGCTGCCACATGAATCTTCTTCAAGGTCCTAT

[1197] GTGTAGATTTTCTTGACTTGGCCATATTATTTAGGATTCAGATATAATAACAAAATAGA(SEQ IDNO.585)

[1198] EGFRvIIIDprobe0586:TGTTAAAGCATAACATGAAGGCATTTAAAAGGGTAGAAAGCACATGATTTACTAAAACCA

[1199] TAAATCTTATGACCTGAAAGTTTCACCTAATCTCTTAAAAAATACCGTACTAAACCCTGA(SEQ IDNO.586)

[1200] EGFRvIIIDprobe0587:TTGAAAATCAGAGCTCAGACATACAGCCTGAGATGCCAAAAAATGGCCAGGCTTGTCTG

[1201] TTGAGAAAGCCATATGTAACTAACTGTTTGGAAATTCAAAATATATCTTATCATTTTAAAA(SEQ IDNO.587)

[1202] EGFRvIIIDprobe0588:ACATCTTTCTTCTAAAGACAATCATCTTGGCTTCAGGAATGAGGCTAGTAAAAAGTGAAA

[1203] TACTCCTACTTGTGGAAGAAATCCTCATTTTAACCATGAAGAACTGAAAAATGCATTCTG(SEQ IDNO.588)

[1204] EGFRvIIIDprobe0589:ATGTTGATGGACCCAACCTATATTTGGGTATTTTATGATGTACACAATATACTTTTGTATATG

[1205] AGATTGTTATTAAATGTGACTTTGCTTTTTCAAGACATACAATGTTCCTCCGGGGGT(SEQ IDNO.589)

[1206] EGFRvIIIDprobe0590:CAGGCACTGTGTTTAGCACTTTGTCCTGACCTCATCTGACTTCTCAGCTGTCCCTGAGAG

[1207] GTACCAGTGTGCAAGATCGCTGAGTTGGCAAGTGATAGTGACAATATTTTCACCCCAATT(SEQ IDNO.590)

[1208] EGFRvIIIDprobe0591:TCTAATTTAAAGACCCCGATTTCTAGTTTTGTTTTGTATTGGATTTGCACAATTTCACGTTC

[1209] TGAAAGAGGATGCCCTCAACTTTGCAAAATGGGCCTTTTGAATGAAAAGGATCAGTCA(SEQ IDNO.591)

[1210] EGFRvIIIDprobe0592:TGTCAGGAAAAGCGCTACAATGATGAAATATGATAAATAAGTCAGTCTTTCATCTGTAATT

[1211] ATCTACTATGGGGTAAAAAGTGATGAAAACTACCATCTTGAAAGGTTCTGGTGATAGTG(SEQ IDNO.592)

[1212] EGFRvIIIDprobe0593:GTTCCTAATGCAGTGAAAGATGTGTAAGTCAAAGATTTGTAACCAGCCAGGGAATGAGA

[1213] GGCGAAGCCATAGCTGGTGGCGGGGGCCACATCTGGGTGTGGGGAGGCCACAGTTGGGTTG(SEQ IDNO.593)

[1214] EGFRvIIIDprobe0594:GGGGTGGGGCCTGCAGTTATCCACACCCCTCCCACCTCCCTTCGACAGTACAGGCTTCCT

[1215] GGTTACCTTCCAGAGAGTAAGGCCAGGGAGAGTTGAATAAGTTGAGAAATGTCATGTCGA(SEQ IDNO.594)

[1216] EGFRvIIIDprobe0595:AGCTATTGGTGGAAAGAGTTCCATTAATTGACAATACAAGTCCCTACTACATTCTAAAATC

[1217] TGGTCCTGACTAGTGGCAAGCCGGGCCCAGGAGTAGCACTTAAACAATGGCAGGCTTGT(SEQ IDNO.595)

[1218] EGFRvIIIDprobe0596:GTTGCTGGCAGGATACTTCAGCCTCAGAGGAGCTGTGTGCAGCTGGGGAGACTCACACT

[1219] CAGAGGATTTCAAAGCAGAGGGCATCTCGTAGAGCAACTTATCCAAACCCTGACCCACTGT(SEQ IDNO.596)

[1220] EGFRvIIIDprobe0597:cacacacacacacacacacacCCTgagagagagaaagagagagagataactaaagagagagaACTAAAGTTTGGCAAAA

[1221] TAATACATGCTCTAATGAAGGTTTATTAATGATTAATCTAC(SEQ ID NO.597)

[1222] EGFRvIIIDprobe0598:GAAGGTTTATTAATGATTAATCTACTCCTAGCatttcctagtccactctatctccttaaaaaaaaattctggttgcagccca

[1223] ctaacttgattgtacagctgcttaatggatagcaggct(SEQ ID NO.598)

[1224] EGFRvIIIDprobe0599:gtaatttTCAGAGAACTGTTTAATGCGGGCTACCTCTGTTCTTCCATGCTGCTTGTGGTTCCTG

[1225] CTCTGCTCAGGACAGAATGGGGAGGAAAACAGGCTCTGCGGCACAATATTGGCAAG(SEQ IDNO.599)

[1226] EGFRvIIIDprobe0600:TGAAATTTTGTAAACCGGCCCTCCCTTCCTTTTGCATTTGGTCTGAAAATTCAATTAGATG

[1227] CTGAGTCCTACAATGTATTTGAGAAGCCCAGGAGTGCCCTAGAGGATGAGACTGGGTGG(SEQ IDNO.600)

[1228] EGFRvIIIDprobe0601:CTCCCTGTCAGGTTGAACATTTGCCTTAATTACTTTGGCAAGATTTGCATCAGTGGTATTA

[1229] GTCCCTGCCTCACTTGGAGGCCTGCACTTAAGTGGCCACATTCAGGCTCCAATTTCCTG(SEQ IDNO.601)

[1230] EGFRvIIIDprobe0602:GTGATTTCATAGTGTAGGGCACTTGCAATCAAAACTAGGCTTAAAGCCCAACCCTCTTAC

[1231] ATTTTACCCACCCCCACAAATGCAGCAAATAAAATGACTCTGATTTTCATTCCCTAGACC(SEQ IDNO.602)

[1232] EGFRvIIIDprobe0603:TCTTTTCTATATTTATTACATTATTGTTAAGACAGTTTTTGAAGAAAGCTGTTTTATTTAACA

[1233] AAATAGCTTTATGGAATCAACTTCATATATCTTCTCCGCCAGATCAAAACAAGCTCG(SEQ IDNO.603)

[1234] EGFRvIIIDprobe0604:TAGTATTAGATGTCACCGAGCACCATGACAGGCAGATGAACATCATCCCTGTGCCCGGCT

[1235] AATGATAGCTCGGCCTGCCCCGGCGTCAGCCGCTCCTGGCAGGGCCAGCGGGCGGTGTGG(SEQ IDNO.604)

[1236] EGFRvIIIDprobe0605:GACCGGCACCGTATCTCCAGCAATTCGCAGATAACAAATATGGTTCTGATGATGTTACTAA

[1237] AGATCTGTCCCTTTCAAGATTGGATTAGACATTAGGAATTTGGAGGGCTTTTTATTGCT(SEQ IDNO.605)

[1238] EGFRvIIIDprobe0606:AGCATTTTTAAGAATAACCAATTAGAGTATTGATTCTAAAGTCTGAAAGCCACATGGACA

[1239] GAGTTCATGTAATTGGCTACTTTATGTGCCTCTTCCTAGATTGCCCTGCATTTTCAAAAC(SEQ IDNO.606)

[1240] EGFRvIIIDprobe0607:AAGAGCCTTTCTATTTTAATCAAAAGAATCCAGAATGAAATGAGGCTTTGAAAACTCAGC

[1241] CTATGTTTGTCTTGATTTCCTTAACTGACATCTAGAAGAAAATATGAGCTCAGGGGTCCG(SEQ IDNO.607)

[1242] EGFRvIIIDprobe0608:CTGGGTTCCTTCCAGCGCCTAAGCCTGTAAGCTCTTCCTGCTGGAACCAAGCTTTAAATG

[1243] CACTTGTCAGTCATGTCCCATGAGAATAGATACTGCCTTCCATGTTTTTTTGTTCTGATT(SEQ IDNO.608)

[1244] EGFRvIIIDprobe0609:TCCGTGTTTGAAATGATGAAAATCATTTTTCTGTGCTTTTTAAAAATGGAATTGCTTTTGT

[1245] GTTGGGAATTGTGCTGTTCATTTTTACTCTACCTCGTTTTGGAATCACTAATGTGGCCA(SEQ IDNO.609)

[1246] EGFRvIIIDprobe0610:ATTTATAGCCAAAAATCAGTATCGTAGAGTGAGCAATGAATGGCATGGTGACTGTGTGAG

[1247] CGAATTCATGCCCTCCCTCCCCACCGCTCGCCCCGCGTCTCAGTCCTCAGTGATGGTAAA(SEQ IDNO.610)

[1248] EGFRvIIIDprobe0611:CAGAATGAGGACCTTCTCCCGACCGTGATGCGCCTCAGCCCTACTTCCCTTGTCCTTTCCT

[1249] ATCATAAAATCTTCTTTCATAGAAATGGTCATTTCTGTTCATATCTGTGGACTGTAAAT(SEQ IDNO.611)

[1250] EGFRvIIIDprobe0612:AACAAGGAAGTCATTTTTGAGGTGAAAACTGCACTTAGACTCATTCCAATTTTGATGGAA

[1251] ACTTTTAGCTGGTGGATGGCATTTTGTTTTGTCTTAGTTTTGCAAGGAGTTATCTTAATT(SEQ IDNO.612)

[1252] EGFRvIIIDprobe0613:TAGGGAGATGAAACTAGTCTGTGATCCGAGGTCTCACTTCCATACATTTCTCTCGGGCAG

[1253] TGTGGCTGCCTGAATCATGCCTGGATGCCACAGGTGCTTAGCCAGCTGGTCCTGTCGTAA(SEQ IDNO.613)

[1254] EGFRvIIIDprobe0614:CTGTCACTGGTAGCTCAGGGAGTGCAGAGGTGCCAGCAGACACTATGAAATTGGCCTCG

[1255] TAAAGCATCAGTTATGTTGTGATGGTGGCAAAGCTGCAGGCGAGATGGGAAGTGCAGCCAC(SEQ IDNO.614)

[1256] EGFRvIIIDprobe0615:TGAGAACTCACAGTAGAGCGTGTGTAACGTAAAAAGATGAAACCCATTGTACACAGCTG

[1257] TGTACTGCCTCCTTGAAGTCAAATTTCCCCCATTACCAAGGAAAAGTTTTTTCTGAAGGGG(SEQ IDNO.615)

[1258] EGFRvIIIDprobe0616:GCTGCTTGACAGGATGACATCTGGTGATATCATTTATTCCTTTGGAAATCAATCTGTGGAA

[1259] GTGAGTTTCCACTGACTGATGAGGAGAAAAATGAATTGGCTTCACCCAGCATCCAGCTT(SEQ IDNO.616)

[1260] EGFRvIIIDprobe0617:CTTATCCTGGGAGAGATAGCTCTTGGTCTGTCATCCACGCAGCTGCCTGGTGCAAGAGCC

[1261] AAGTTTGTGCAGCCTGCAGAGCACTCTTCCTGAGCTGTGGGCTGCCAGGTCGGGGGGCAG(SEQ IDNO.617)

[1262] EGFRvIIIDprobe0618:GGGGGGCCTCACTGTGCAGCCTCCTGCCACCCACTGATCATCTGGGGAGACTGGCCTATC

[1263] CTGTCAGGAGACGCAGTTGCCCAGACGTTTTCAAGGGCCTAAGATGTAGGCAGTTGATCC(SEQ IDNO.618)

[1264] EGFRvIIIDprobe0619:ACAGATTTTTGGAGAGTCCTTGAGTTGGAGATTACAGGTGACCTCAGAGGAGGGAGTGA

[1265] GAACATCTGGGTCATGGGTTTCTACTAGGAGTCCACAGTGAAAACAAGAAGAGGAATTTAC(SEQ IDNO.619)

[1266] EGFRvIIIDprobe0620:GACAAGACAGTCCAGCAACTTCCTTTCTAACTTCTCCTTTCACATATGCTGGATACTCCAA

[1267] GACTTTGCATTTACATGGACATCACAGATCCACTTTGAGAGAAGTAGGGTAAAAAGAAA(SEQ IDNO.620)

[1268] EGFRvIIIDprobe0621:TAAATACATAGTGCTTTAGGTGTATTTCTATACATCTTAATTGATATGGGATTACATTTTCAC

[1269] TTGTGTTTACTGTACAGACTCTAGACAGATCCTGCTCTTTTGCAGGTAAAACAAATA(SEQ IDNO.621)

[1270] EGFRvIIIDprobe0622:TTTCTTAAAACCTAGAAAGACCCAAAACAATTTAACAGAAACATTTTGGACCATTTTGGA

[1271] CCTTGGCAGTTAGGCCCCAGTGCAGCAGCGGCAACCATAAACCTCTCCATAGGTGCTGAA(SEQ IDNO.622)

[1272] EGFRvIIIDprobe0623:CCCAGGTGATCCCTGGCACCGGCAGCCTTATGTCAGGGCTCTCTTATCGCTGGTTTTTATT

[1273] TCTCCTAATAAAAGTGATTAAAAGATTCATCTTTTAAAGAAAGCAAGGACACAGAGGTG(SEQ IDNO.623)

[1274] EGFRvIIIDprobe0624:GATTCTCCCTGACGCTAGCACAGCTCATGCCCAAGCCACTCCTGCAGGGCTCTGGTCTAA

[1275] GTGCAAAAGCTGGAAAAGCTGCAGGTCCCGCAAGACACAGAGCAACCCTGCAAGCCAGGT(SEQ IDNO.624)

[1276] EGFRvIIIDprobe0625:CACCTTCCCTCTTCTCTGCTGTCCGACTGGCCCTCCACCATGTGACATTCAAAAGCTcaagtt

[1277] acttaacctctcaaaactcagcatccttttctgtacagtggggaagatactggactg(SEQ IDNO.625)

[1278] EGFRvIIIDprobe0626:ttgtgaggattaagtgagGAGAGTGGCCCAATGAGTTGACAGTTATTACTGTCATTGTCATTATTTG

[1279] CCTTCTCACAGGCAGGCGTGCCACAGTCATTTTACTGAAGCTGCTTCAGTGG(SEQ ID NO.626)

[1280] EGFRvIIIDprobe0627:GTCCTGAATTAGGCCCTGTCCTTTGGGAGAGACAGTCCTGGTTCAACACACAGCTCCCTG

[1281] CCCAGGGCAGCTTGGGAGTGTGGGCCAGTTTCGCCTTTAGAACCACAATCTCTCTGATATG(SEQ IDNO.627)

[1282] EGFRvIIIDprobe0628: TGCAATGAGAGAATTAATTATAGACTCAAGGATTGcatgcacacacacacacaaaacacacacacaac

[1283] acacagttacacacacacatgctcacatacagaaatacaca (SEQ ID NO.628)

[1284] EGFRvIIIDprobe0629:agcacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacacaga

[1285] (SEQ ID NO.629)

[1286] EGFRvIIIDprobe0630:acacacacagagagacacacacacacagcccagggagacacacacacacacacacacacacacacacacacacacacaca

[1287] cacagagagacatacatacaatacacaga(SEQ ID NO.630)

[1288] EGFRvIIIDprobe0631:gatacacacagagacacagatacagacacagacagacatacacacagacacgggcacacacagagacacacagacacacacaggcacaca

[1289] cGTGCAGATAAGGTAATATTAGCTAGTTCA(SEQ ID NO.631)

[1290] EGFRvIIIDprobe0632:GGAGGAGAAAGAGATAAAGATAAAGTAATATTAGCTAGTTCAGGAGGAGTGAAAGAAGC

[1291] CTTGTTTTTCTCCACTTTTTATAGAAGAGAAAGTGAAGATTCGATTTGAGGTGAGTTCAGC(SEQ IDNO.632)

[1292] EGFRvIIIDprobe0633:ACAAAAGCGTATCCCAGGCCCTCTGGCTCCAACTGCAGCCCTTTCTACCTCATTCCCAGA

[1293] CCCCACCTAAGCCTTTTCTCTTCAAAATCTTCTCAGGCACACTGATACACATACCTCAGA(SEQ IDNO.633)

[1294] EGFRvIIIDprobe0634:TTTTTAATTCTCCGGTTGTGTTCACCAGGTGCTTGGTCATGATTAAGAATTCCGTGATGTG

[1295] TACCCCATGTGTTTAAATTTGCTGCTGAGTTAACTTTGTGGCGGCCTGTGGACTAGACC(SEQ IDNO.634)

[1296] EGFRvIIIDprobe0635:TCTGCACATGCAATGCAGAACGGCAGGGCCAGATTTGAAATCCTGCTATCTTTTCGGCTG

[1297] CCTTGTAAAAATAACATCAGGCGATGGGGATACGATGCCAGAGGTCACCTGTGATAAGTT(SEQ IDNO.635)

[1298] EGFRvIIIDprobe0636:CTGTTTATGGCCATTTTACTTCTAGGAAGACAGGAAGTGTCAGGATCTCAGGGATCTAGG

[1299] AAGCCAAAATGTTTTTCCACTCTGAAATAAAGTGACTGACCAGGAGTTCCCGGCCACGCA(SEQ IDNO.636)

[1300] EGFRvIIIDprobe0637:GCCCTGTGGGAACTGCCGCACGGCCACTTTTATGAAGTGGACACGTGTTGGTCCCACTG

[1301] AAAAGAAACTCCCCACCCATGGCTCCCTCACGCTGCAGCAGAGGCCCTGCCACAGCACCTG(SEQ IDNO.637)

[1302] EGFRvIIIDprobe0638:TCAGCCCCTGCCAGCTTGCAGGGGCGCAGGCGCAGAGCGGTTTGTGCCCTTGCTGGAGC

[1303] CAGGGAAGGGCACAGGGTCCCTCCTGGAGTCATGGGAGGTGCAGCCGAGGTTCTATATTAA(SEQ IDNO.638)

[1304] EGFRvIIIDprobe0639:AATACAGAGGCTAGCACATGTGCTTGGGGAATGCAGCTACAGTAGTGGAATGAAAGTGC

[1305] TGTCCGTTCCTTACCCCCCCAGCTCCTCACCTGTCCTCCACACGCATATCCCTGGCTCCCT(SEQ IDNO.639)

[1306] EGFRvIIIDprobe0640:TTCCCTAGTAAGGAGACTGAATTGAAATTGTGGCTTGCCCGAGGCTGCATACCTGTGCTC

[1307] TTTCTGAAGCCCAAGTCACTGGCTCTAGAATTCTAACCTGTGAGGAAGCcactgaggatg(SEQ IDNO.640)

[1308] EGFRvIIIDprobe0641:tttgtcaaaatacatatttctgtgccttgccccagttccacggcccaggaatctgcagttttcacaagcacccccaggtgattctggtgGTGTCT

[1309] TTGCACTTCTTCAAGGCAGTACTGC(SEQ ID NO.641)

[1310] EGFRvIIIDprobe0642:CTGGAACGCAGAATCCCAGCCTCCTCTATCCTCCTTGCCTAATGGCCTGGATGCTCTCAGA

[1311] TCTACAGGGGAAGGGAAGGTCACACAGTCATCGCAATAGTAACCTCAGCTGATAAATCC(SEQ IDNO.642)

[1312] EGFRvIIIDprobe0643:TCCCCCATAAAACTTATTCCCcagtgttttttaataggaaacaataaaactgtaaccagcccaaatatccatcaaagagaaaatg

[1313] gagaagtaaatcatcgcacattcaCCTGGACCAGA(SEQ ID NO.643)

[1314] EGFRvIIIDprobe0644:TCTATTGTAAAGCCAATAATACTGAAgccccttccaaggccctgggagtcctaacagtgcactggcagtgtctataatttat

[1315] attatgaaatttgcataaggaaaacattttGTCTCATT(SEQ ID NO.644)

[1316] EGFRvIIIDprobe0645:TGTGCAATTTCTCCTTCTAAATATACGTGTCACTTTGTACCTGATTTCTATAAGACCCAGGA

[1317] CCTACAAACCCTGTGTCTGCCCCTGCAGCCACCCAGGGAAGGACTGCACAGCAGCAAG(SEQ IDNO.645)

[1318] EGFRvIIIDprobe0646:ACAGATTGCCATGGAGCATGTTGTGCCCAACTAGGGACAGCGCAGATAGATTCTGTAATT

[1319] TGCCTAACAATGTCTATAGGATGATCCCATTTGTCAAAAAAAAAAAAGAACTGGGCTTTA(SEQ IDNO.646)

[1320] EGFRvIIIDprobe0647:TTGATGTCACCTAAATGCACCTAAACTTCTTTTTTGCCCCATGCTCTTCTGTACTCTTGATC

[1321] TTTCCCCAAATTTTTAAAAACATGACACTCATTCCCTTATTTTTCCTACTTAGAAAAG(SEQ IDNO.647)

[1322] EGFRvIIIDprobe0648:TGTAGATGGTTTTATCATAGGAAGTTCAAAAAAATTAAAATATAATGAAAAATACTCAAAT

[1323] AGTGCCTCACAACAGTAACTACTGCTAACATAAATAAAATCCATATTTCCTCTCATACA(SEQ IDNO.648)

[1324] EGFRvIIIDprobe0649:GACCCCAGAGTTGCTTTGCCTGACAGTGTAGTTGATGGAGAAAATAATCTTTATCCTTAG

[1325] CCTCCATCTGGTTGCAGACCATAAAGACAGGGAAAAAATGAGGGTGTTGGTAGCTTCGTT(SEQ IDNO.649)

[1326] EGFRvIIIDprobe0650:AGAAACTGAAAGCTCACTGATTTTTCAAACCTAAATAGCCTGTGTTTCTCCAAATAAC

[1327] TAATTTGCAGCCTTCGGCAGCCAGGACTGGCAGGGATGGGGCTAGGGGGACTGGGGAGAA(SEQ IDNO.650)

[1328] EGFRvIIIDprobe0651:CTGCTCTCTCCTGAGGGTGGTCTGACCCGACAGCACGCATGACCTtcccacagtcaggaactgctcag

[1329] agacgtgatggcaactccatagaatgaaatactcttcagccagtaaaatgta(SEQ ID NO.651)

[1330] EGFRvIIIDprobe0652:ttttggataaattttgctttaaaaaactttactatatgttgttaaatgaaaaaaaaccttaaggcatcagaaattatgtgcagtaaaatctcactttgta

[1331] grandfathercctgtt(SEQ ID NO.652)

[1332] EGFRvIIIDprobe0653:tactacgtatgcataaaaagaatcctgagaaatataAGTACTGTATGCATATTGTTGTTAAGTATTTTTTCTGTTT

[1333] GCTTATCTATAATTCTAATTTTGCTTCAAAGAACAAGTTACTCC(SEQ ID NO.653)

[1334] EGFRvIIIDprobe0654:GGCAATATAAAAATAAAATAACTAATTTGTCTTGTCATCAAACAGATAGTAAGAACAGGC

[1335] AAACCTGGCCCTCCACACTGCCAGCCTTTTGTGATTCAAGGCTTCAGTTTCCTCCACTTG(SEQ IDNO.654)

[1336] EGFRvIIIDprobe0655:TTAAAAAGATTCAACAAAGTAGTTGAAATAGTATGTGAACCAGTAAACCCTAAAAGGTGT

[1337] CCAGTGTTGTCTGTGAGCTAATTAAGTGATTTGATTCTGACTCCCCGAGTCTTCTGATTT(SEQ IDNO.655)

[1338] EGFRvIIIDprobe0656:CGAAGCAGTGGGGAGTCAGACAGGAGCCTCAGGTGGCCTCTCCTGAGAGGCCCTGGAA

[1339] AGTGATGAGAACCTGGCCTCTGGCAGCTCTTCATAAACGTCCATGTTTTCCCTCTACTCTCT(SEQ IDNO.656)

[1340] EGFRvIIIDprobe0657:CACTCTTTTCCCAGGGCCTCAAACAGAAGATGAAAATCAATTTCTAAAACAGCCCTCTGT

[1341] GTGCTCTCTCGTATCTCTCCTTTTCACACATCGTGGTGGTGGCTTTCTCTGTGTTCCTCT(SEQ IDNO.657)

[1342] EGFRvIIIDprobe0658:GTTGATTCAGTCTCTGGAATTAACGGATCAGGATTCCATGCCCAGAATGCTACAAAGACT

[1343] GTGCTTGAGTTCTCCCACATCTCACTCAATTACACAGAAGTTTCAGATTATGTAACAGAT(SEQ IDNO.658)

[1344] EGFRvIIIDprobe0659:GCTGTGCTGGGTTAGGCAGAGCCATCTGACTTGTTTTGCTTTATTTTAGACCATGAGATGG

[1345] GTGAGTTTTTCTTTTTAATGCCACATTCTTTTAAGAATTAAAAACCTCCACTTGGCTGT(SEQ IDNO.659)

[1346] EGFRvIIIDprobe0660:CAGCATTGGAAATCAGAGTGATGGTGCAAGCCCTGATGAGGACAATGTCCTTGTCTATGA

[1347] AAAGGTGAAATCATTGCTTGAAATCGCTAAGCAGGACATGCAGTCCCAGATGGAGGGGGG(SEQ IDNO.660)

[1348] EGFRvIIIDprobe0661:AATTCGGGAGCTGGTTGGAAAAGAGTATTTGGCACTTTGCAGCCTTGAGAGGTGCAGAA

[1349] GAGACACCGAGGGGTTCACCACCAGAGCCACCATTGTCAGAGAGGCGTCCAGCTGTGTCCA(SEQ IDNO.661)

[1350] EGFRvIIIDprobe0662:CCTGGGACTCTGCCTTCAGGGCTTCTTGCCTGGCTGGGAGCTGCACAGGCAGACTCCTG

[1351] GGACGGTGTGCCGACAGCTCTGGGCACCCCCTTCTAGGATCTGATTCCTGAGGAATCACAA(SEQ IDNO.662)

[1352] EGFRvIIIDprobe0663:TGTGGATTTCACAATCACTTCCAGTGTCTTTTGCCAACCTCTGTGAACAGATGTGCAATTA

[1353] AAAAAAAAAAAAGAAAGGGGCCCAATTCTCAACACTGTAAGTGGAAACTTTTTAATGGA(SEQ IDNO.663)

[1354] EGFRvIIIDprobe0664:AAAGGATAGGCTAATGAATTGAATTTGAAATCTGAGACAGAACCGATGCATCAAATGTGC

[1355] TGGTGTTTACAGATAATACAAGGGGGGCTGCATCTTATGGTTTCAATCCTTTTTTAAATT(SEQ IDNO.664)

[1356] EGFRvIIIDprobe0665:TTTGTTCTGAGAGACCCAGCCAGCAGACTGCCGCCAGTCTTGTCAGAGATGTCAGTGGT

[1357] GGCCACTCTGAATGGAAAGCAGCATCTCTCAGCATCTCTGAGGCACTGCTCCTCAGCGGAG(SEQ IDNO.665)

[1358] EGFRvIIIDprobe0666:ACTGTGGTGGCTTTGCCTTTCAGCACGCATCCTTTCTACGATGCCTGACAGTGCCCAGGG

[1359] AATGGGCAGAGCTGGGAGCTCTGAAGCCCTTTCACCTAAACCACCCTGGGTCACCTGACC(SEQ IDNO.666)

[1360] EGFRvIIIDprobe0667:TAGTTTTCCTCCCAATTTTAATTATGTCAGGCACTTCACAAAGGCCTCCTTGGGGACACCA

[1361] TGAGCTCACTGTCATCAGATTGCTCCAATCACAGCTGTGGCTTGCACACAACCGCCATC(SEQ IDNO.667)

[1362] EGFRvIIIDprobe0668:TCTGCCCCAGCAGATGCTGTGTGTAAACAGTTGTATTAATTACATCTCAAAAACATGGTTC

[1363] TTGCCAGATCCTCAGGATTTGGGTGCAGCCTCTGAGGTGGGTGGGAGGCCCTCGAGGGA(SEQ IDNO.668)

[1364] EGFRvIIIDprobe0669:GAAATGTCTGCAGGAAATTCTTCCCCTACGAGAGGTCTGTTTTCTAAGTTATCTAAGAGCT

[1365] ACTGCAGCTGTTTACTGCAGAGTGACCCTGCTCAAAGCTGTGGTCACCCAAGGCTTTGA(SEQ IDNO.669)

[1366] EGFRvIIIDprobe0670:AAGGGGACCTCCACTTCCGCCCTGGGTGGAGCACCGTGCTGGAGACCCACGCCTGCCAA

[1367] GGCCTCATTGTCATCTCCACACGCCGTCCTTGGGGTGGGCCACTCCTGGGACACGCAGACA(SEQ IDNO.670)

[1368] EGFRvIIIDprobe0671:GGAAGCCGGCCACCTGAGCCACTCGGAGGCTCTATCCAGAGTCAGCTGCCAAGCCTCAC

[1369] GTCACACATCACTGTTAGTCTTGGAGGGCTGGCGGGGCCCTGAAGTCAAttgaacacttgg(SEQ IDNO.671)

[1370] EGFRvIIIDprobe0672:atgacagggaacttgccactgccagaggcaatatgctccatttttttgacagttccaacaatttttctttaaactgTCATAAAAAATTGCT

[1371] GCTGTGAATACCAGTGTCGGCGTCCCTGC(SEQ ID NO.672)

[1372] EGFRvIIIDprobe0673:CTCACCTTTACCTGGTGCTTTTCCACCACACAAAACTGTTTCTCCTCGTGCTGGCCTTGG

[1373] GCTTGCAGACAGCTGATTCTTCTCCTCCCGCGGCTGAGCAGCCTCCTCCGAGCAACCCTC(SEQ IDNO.673)

[1374] EGFRvIIIDprobe0674:TGACAACTCTGCTCCTTCTGACAACCTCTGCAAGGGCTGCCAGATGTGAACAAGGGGCC

[1375] CGGGCAGAAGGTATCCAGGAAGACTGGAAACTCGAGGAAGCCTGCCCTGTCCTGTCCACCA(SEQ IDNO.674)

[1376] EGFRvIIIDprobe0675:GACTTTACGCTTGCGTCACTGGGCTTTGGGACCTAAGTCCTCGTCATTTGTTCCTTTTGCA

[1377] GTTCCTACTGTTCTCAGCACTTCCTTCCAGCTTACTGAGGTACACTCAGATGTGATATG(SEQ IDNO.675)

[1378] EGFRvIIIDprobe0676:CCATCGGTACAGACACAGTTCTGCTCCAGCATTTCCCCGTGTTCTTTCTGTCGCTCTATTT

[1379] ACTGAATTACCGTGAGGATGTGGAGCGAGGCTGAGTTCTGTATTTTAACACCATTTTAA(SEQ IDNO.676)

[1380] EGFRvIIIDprobe0677:TTCTCACCTACTGAGAAATCCATCCTCTTATCACTGTGCTTTTTTTAACCTGTCACGAATCC

[1381] ATGAAATCCTATCAGCCAGCCTGCATACTTCCTTTTAAGGTGCAGTTGAATCAGGAGA(SEQ IDNO.677)

[1382] EGFRvIIIDprobe0678:AACTTGCCGCACATGCTGCGTCCGGGCACAGCATTGGCTGAGGCTGCTGCCCTGACCTG

[1383] TCCGCTTTGTAGTACTGCCCAGCTATGAAACAGGTTAGCCACACATGACCTGCATTTAGGA(SEQ IDNO.678)

[1384] EGFRvIIIDprobe0679:GTAACAAGTCTGTCTGTACATGCACATACAGCAACTTTTTTAAACTGTCTATATTTTTTCCT

[1385] GAGATAGGTATTTATAATATCTCCATCTTCTTTCCCATTTTGAAACTTAGAACAAGTT(SEQ IDNO.679)

[1386] EGFRvIIIDprobe0680:TGCCTGTCAACAGTTCTCCACAGCATACTGTGTATTCTAGGATTTTCTAAGGTTGAGCAAC

[1387] GGAGGTTCAGCAATTTTGACTTAATTTCTTCCCATCCCTTTTCCACGCAGCCCAGAAGC(SEQ IDNO.680)

[1388] EGFRvIIIDprobe0681:CTTGGATCACGTGGTGAGGGGAAGAGGTTGTGCTATGTCGGGAAACTCTGTATCGAAGC

[1389] TCGGCTCAGATCATGACATTCTCTTGACTAAAACCCTCAGTTTCCATCAAACTTGTCACTC(SEQ IDNO.681)

[1390] EGFRvIIIDprobe0682:TGGCATTAAAGCCTGTCACTGTGTGGCTCTGAAAACCTCTCTGAACGTGTTCCCTGCCTC

[1391] TGCCCTGCAGGTCCCTGTGCTCCACAGAAGCCCACTTATGTGACCCACCCCCACTCATCA(SEQ IDNO.682)

[1392] EGFRvIIIDprobe0683:CCACCTTCCCTCACCCAGAGCCTCAGCTCCCCACTCCCACCTGTAAGACCCCTACTGGAA

[1393] AGATTCCCACCTGCCCCTCAAGATTAATCTCCAAGGACATTTCCAAATTCCTCTCCCCAT(SEQ IDNO.683)

[1394] EGFRvIIIDprobe0684:CTCTCAGCCAGATGGCTTTGCTCCCTCCAGGAACCCCAGCCACCTTCGACCTCCAGCAG

[1395] GGCACTCCACTCCACATTCTCCTGGTCTGTCTGGCTCATCTTACCTGAGCCATGCTCTCCA(SEQ IDNO.684)

[1396] EGFRvIIIDprobe0685:GGTGAAGGACTATGTCTAACTCAACTCTGCTTTAAAAGCAGCTAACACATTGCTCTTTGC

[1397] ATATTGTTCACTCACTAAGTTGAACTGGACTTGGACATGCACACTGAACTGCAGCGTCTG(SEQ IDNO.685)

[1398] EGFRvIIIDprobe0686:CTGCTTCTTGGTGGCCCAGCTCGTCAAAAGAATAAGATTTCAGCAAAACAATGTAACAAT

[1399] TTTTTTTACCAAAAGTAATGTTAACAATATATGGTTTTCCCCTGATGTTTGCGTCAAAAT(SEQ IDNO.686)

[1400] EGFRvIIIDprobe0687:GCTTTTTGGAAAAAACATTTTTCAACTCTTTAGGGTCAGAATTAAGCAATGAAATTTATAT

[1401] ACCACATGTATAATGTGTATGTTTATCTAAGTATCTGTTCatttatatatcttaaatag(SEQ IDNO.687)

[1402] EGFRvIIIDprobe0688:aaattttaaaaatttttttaaaaCTCCTGATAAACATTCTCAGGAGGCACACTATGTAACTGTTGGTTGAT

[1403] ATACCTAGCTAGATGGTGAAATCAGATTTTGTTTAAAGCATGGAGGAGA(SEQ ID NO.688)

[1404] EGFRvIIIDprobe0689:GGGAAAAATTAAATCTTGCAGATTCTGCAGTCCTTAACATCTTTGAAAGAGGAACATTTC

[1405] AGACAATGTAATAAGAAGGCCACGTGCTTTGACTTCTGTAGATTTTAAAAATACTTCTGT(SEQ IDNO.689)

[1406] EGFRvIIIDprobe0690:ATAGTTTCTTCTTCCTTTGAAGAAGTTTGGGGAGTTTGGGAAGATGGAGAAAGATATAAG

[1407] AATAGACTCCCCATATGGGTCATGAATTATCTTTTTGCATCAGAACTCTTAGTGCAGTTT(SEQ IDNO.690)

[1408] EGFRvIIIDprobe0691:CAGTATTTTCTTCCTCAGGAGGGTGAGCTGCTTCCGAATGTCCTCCCCTTCTTTGAGGCAT

[1409] CCTCTGTTGGTGAACTTTGAGAGCATCCATTTATGAAGTTGATGACCTTTCCCAGTCTC(SEQ IDNO.691)

[1410] EGFRvIIIDprobe0692:TGCAAGCCCTTCAGTGTGTGTCCTCTCTGAGCAAATCTGAATTGTGTGCTTAATACATGGA

[1411] AAGGGATTTGGGAGGGTTGCTTTTTAAACTGATTTCTTAATTAATATTATGGTTTAGTT(SEQ IDNO.692)

[1412] EGFRvIIIDprobe0693:AACTAGACAGTCTCATTGCAGAAGTGCATAACCATAATATGTCTTCAAATATATCTCCCTTC

[1413] CTAACACCCTGTAATATACTTTTGTAAAGATACCCTTACAGAATGTGATCCACCATTT(SEQ IDNO.693)

[1414] EGFRvIIIDprobe0694:ATGAACCTGCAGCATTGCATTCAGAGACTAAGTGAAAAGCTGGCAGATTTTCATTTAAAG

[1415] CACAAGCTAAGGAAGAAAGCTGGTCTAGAAGGAGCTACAGAAGGGTAATGCTTAGGGAGG(SEQ IDNO.694)

[1416] EGFRvIIIDprobe0695:GAATGATGTGCCTGTGGGTGGTGGTAGTTAAATCTAACCAAAGAATGATGTCGTGGGTGT

[1417] TTGGATATTGGATGGTCCACATTGGGCCACATTCTTTCAAACATAAGAGTCTGTAGAAAT(SEQ IDNO.695)

[1418] EGFRvIIIDprobe0696:ATGACCTGTAAAAGACTCTTAAATATTCTGGAAACTGTTTCTTCCTTGTCACATCCTTATAT

[1419] ATACTTGAACCTATGCCTACCAGACATGACATGTGACTATTCATACAGATTTCATCAT(SEQ IDNO.696)

[1420] EGFRvIIIDprobe0697:CTCTGGTTTAAGAATAAAGGATGCTGCATAGAAGGCTCACATCTTTTAATTCACAAGACT

[1421] GAAACTGTTCTGAAATGACATTGTTTCTAAAAATTCATTACTTGCATTATATTCATTTTT(SEQ IDNO.697)

[1422] EGFRvIIIDprobe0698:ATTTTTCCATGCCAGAAGGGTAGAAGTTCCTGTGCTCATATTAAGAAACAGCAATGTCAA

[1423] TCGAGGCCCAACTCAAATCCAATTTATAGGAGTTATAAAGGGCGTGTGCCTGTTTTGTCT(SEQ IDNO.698)

[1424] EGFRvIIIDprobe0699:AGAAGCAGTGTTGGGCAGCACTGAGTAGGATAGACCACCTGTTGCTACCGATAAAGGAG

[1425] CAGCTTCTCGAATGCTCCTGTCTGGTAGGCACTATCCCGAGTGCTTTGGCCCCTCATCCAC(SEQ IDNO.699)

[1426] EGFRvIIIDprobe0700:AATCTGTGTGGCAAAAGGCATTGCAGGCAATTCAGTGAGGAGACCGAGGCATGGAGAGC

[1427] AAGTGCCATGGAATTCCCTAAGGCCGTGCAGGGAGCAGGTTGCCAAGCTGGGTTGAAACCG(SEQ IDNO.700)

[1428] EGFRvIIIDprobe0701:TCCTCCGTAGGCTCCCAACTCCGCCGTCGCTGCTACTGTGCTGGATGATGCCTGGTAGAT

[1429] GCAGATGTGGAGCCCCAtggattctgagacaggccgggtttcagtcctgccctagctgcc(SEQ IDNO.701)

[1430] EGFRvIIIDprobe0702:tattggctggatgaccttggcaagttgactttcgtgagcctcatttgtctcatctctcaattaagaaaacctagagcctatctgtgggggttatctgaag

[1431] gattccagggatgcatatgg(SEQ ID NO.702)

[1432] EGFRvIIIDprobe0703:cactgtctaccgcatgcggtaactgtttcacaaatgatGAGGAGCGATTTATGTTCTTAGTGGAAATATGTCGGC

[1433] GTGTGAAGTCCCAAAGCTCTGCCCTGCCTGGCTTGATCCAGTGCC(SEQ ID NO.703)

[1434] EGFRvIIIDprobe0704:TAGGCACTGCCCCTCTTCCCCTCTCTCCCAACCCACTGTAAGAGGCTAGGCTGCCTCAGT

[1435] AACTCTGAGGGGCATTGACTCTTTTCATCCAAAAATTCATGTTACTGCCCCACATTTTTT(SEQ IDNO.704)

[1436] EGFRvIIIDprobe0705:CTGTTGTTTTACAACGCAGTAGGAAGTGGGCAGACTGTCAGGAAAAGTGATTTATAGTCA

[1437] TGTATTGCTTGTGCTTTGGCTTCATTTGATCCAATGCAGATCAGCTGCACTCAGAAAACT(SEQ IDNO.705)

[1438] EGFRvIIIDprobe0706:ACTCAAGTGAAAGAGAAAAAGTAACTGAAGGGGGAAATCTGGATGAGTAAGAATTCCA

[1439] GGGATAGGAATATTAATAGCAAGCTTTTTGCCTGATATAGTCACTTTATGCTGCAGGGGTGC(SEQ IDNO.706)

[1440] EGFRvIIIDprobe0707:CCCTTTATAAAGTGCTTGTACAATGGATGTTTGCTTTTGATTTTGGATTTGGAGTCTAATGA

[1441] ATGTTCTAAATTATTATTAGAGGAGCTTGCGGTTGTTACATGTCTGCCTTTATTGCTT(SEQ IDNO.707)

[1442] EGFRvIIIDprobe0708:ATTTTTAGCCATCTCCCCTGATGTCAAATGCTCAGGCAAGAATGATACATTCATTTATAATG

[1443] TGGCTCCTTCAGAAATATACCACATACCTTTTGGTGTGGTTTGTGGCTGAGAAGAGTG(SEQ IDNO.708)

[1444] EGFRvIIIDprobe0709:GGGAATGCACAAGTGGAAAACTGCAGAAAGATTATGCCTTCATCACTTCAAGTATTTGAG

[1445] ATGAAACTAGATCATTTGCTGTTGCTTTTTATTCTCATTCTAAGTGCTTTTCAAAGTCAG(SEQ IDNO.709)

[1446] EGFRvIIIDprobe0710:CGCTAAGATTTTAAAATGGTTTTCTGTTGTTGGCAGAGAGGGAATTACTCTATTACTTTCT

[1447] GATAAAACAGAGTCTTTCATGATCAAAGAGAACCAGGCTCTAGTAGTTCCAGTATCCTA(SEQ IDNO.710)

[1448] EGFRvIIIDprobe0711:ACGTGGACACTAATTGTTTCCCTCCTTTTCTTCATGAAAACAGCTTCTGCACAAATGATAG

[1449] CCTTGTGAACTAGCCATGGGCACAACTGGAGAAGCATTTAGGGAGCTTTAGTGCAAATT(SEQ IDNO.711)

[1450] EGFRvIIIDprobe0712:GAGACCACCTACACATCTGACTCTACAGGGTTTGACAACATCCAGGGTGAATCACAAAA

[1451] CATCAGTCTAATCAGGGCTTATATAGAAAGAGTGAAAGAACTCTGATTTCATCCTAAAGAT(SEQ IDNO.712)

[1452] EGFRvIIIDprobe0713:TATTTATATTAACCATTGTTCCAAATGCATTAACTATTTTAATTTAGTTGTTTTGATTGTTAA

[1453] AAAAAACACATCTGTTTGGTAGATAAGACATAATTTAAGACAAATGTTCTATTTGAT(SEQ IDNO.713)

[1454] EGFRvIIIDprobe0714:AAGCTTTTAGAAACAACTTATTTTTATTCTTTCCTGTGAGATAACTCAGATGTGGAGAATG

[1455] TGACAAAATTTTAAGCATAACATGAGAAGGGCTGACACACATAGATTTCTGTGTGCTTA(SEQ IDNO.714)

[1456] EGFRvIIIDprobe0715:CTTGAAAACAACAAAATTTAAGAATTTGGTATAGGAGTTGTATCAGGTAGTGCAGAGTCC

[1457] CCAGGAGACCTAGAGACCCAGGTCTGGGAGCCTAGCGGCAAGGGCTGAATGTGGGATGAC(SEQ IDNO.715)

[1458] EGFRvIIIDprobe0716:ATCAGCAGAAACTCACAGCCACTGCTATTCCAAAAACCCAGCAGCAGCTCAGTGCAGGG

[1459] CAGTGCTGATAGTACAGTGCCTGCAATCCTGGAGTGGATTTGGATGTGTCAGGTACGCACA(SEQ IDNO.716)

[1460] EGFRvIIIDprobe0717:CGCTCACTGCTCCCCCAGCAGTACGTTGAACAGTGTGCGTCCAGGTGTCTGTAGGGCCC

[1461] CTCGCCCTAACTCACAAAACCATTCTGGGTCAGAAGCCACCAATATTGTCATCATCCTCCC(SEQ IDNO.717)

[1462] EGFRvIIIDprobe0718:TTTTCTGAGAACCCTAGTAAGTCCCTCCAGTGGGGCAAGCCCACCTTTTCCCTTCATTCT

[1463] GTGGCAATATGCCTTCATTTCCTAATCAGTTTTGCCCTGCTCATTCAATGCAAAATGGAT(SEQ IDNO.718)

[1464] EGFRvIIIDprobe0719:CTGCTTTCCTTGGGCACCAATATGTCCAGGGATTGTTTATCAATCTTCAGTTCTGTTTCCTT

[1465] TACATATCCCTCCAAAAATCAGGCCTGCACTGCCTGTGCACTCCACAATCCACAGGCC(SEQ IDNO.719)

[1466] EGFRvIIIDprobe0720:TGAAGGAAATGTTATCTTTGATGTAGAGACTTAAAGTAAAACTCTTCAAATTAATTATTTC

[1467] ATGCAAAAGGCTAGTCCTGACTCTAATTCTAAGACATGTCTCCTAAACTCTGGAAGTCT(SEQ IDNO.720)

[1468] EGFRvIIIDprobe0721:GATGTATCCTATTATCAACATTTATCCTTAATGTGATGGTTTATCATTTATCCTCAAAGCTGC

[1469] ATTGTAAAATGTACACTGTAAAGTGTACATTTTAAAGTCGGTTTTAAAAAATCATAT(SEQ IDNO.721)

[1470] EGFRvIIIDprobe0722:TTAGAGATCCTGGTAAAAATCTATCAAGTCAAGACATTACCTTATTACCCATGGAATTGTC

[1471] TTCAACTCTTACAGTTCAAATATTCCTGAATTGGCTTTCACAATAAACATCCTAAATAT(SEQ IDNO.722)

[1472] EGFRvIIIDprobe0723:GTAAGTAGAAACATATATATTGCCAACTTTGTGCCTTCCCAAGCAAAATTAAAATACAGGA

[1473] AAAGTCAGTTTGTTTTGCCCATAAATAAATATATGTGTGTGTGTATGTGTGTGTATACA(SEQ IDNO.723)

[1474] EGFRvIIIDprobe0724:CATACACACTCAGAAAAGATAGAAGCAGCAGCATATTTTGGCAGCATCTGGTTTATTGGA

[1475] ACTCAAACGTTCTGATTGTGCATACAGACTAGTTAATGTGGTAACAATTATGTATTTCTT(SEQ IDNO.724)

[1476] EGFRvIIIDprobe0725:CCCTGCTCCTTGCCTTCTTTCCCTCCCCAGTTTTTTTCTTCCTGATAGTAGGTGTGTACTTT

[1477] TTTCCTATTTCCATTGGCAAGCCACATGACAAGCAAAACGATCACTCGAAGAATATTG(SEQ IDNO.725)

[1478] EGFRvIIIDprobe0726:TTCCCTCAATCAAGAAAAATGCCCATTGGGTTTTGTTATTTGATGTTATTTGATGACAGAG

[1479] ACCTATTGTTTTTCCATTTTTCTTTTTTTGTTTTCCGTGGCACCTATGGAATTAAGCAA(SEQ IDNO.726)

[1480] EGFRvIIIDprobe0727:TATAAAAAATCTATTATTTCAGATGTTCACGTCTAATGAATTTCATGTGAAATACTGGCAGT

[1481] ATAACCCCAAATAGAGGAAATTTGTGAAGAGTGGATGCTGCAGGGCATGAGACATCTG(SEQ IDNO.727)

[1482] EGFRvIIIDprobe0728:CACAGAGTTCATCTCTTCCAGCATCTTGCATGTCCCAAGCACTGCCCTGCCAGGCAGAGA

[1483] ATGCTGCAGATCACGGCAGTGAATTCCAGTTGTTCAGAGCACATTTGACTTCCAAATTCT(SEQ IDNO.728)

[1484] EGFRvIIIDprobe0729:CAAGGCCACAGATTTGAGGACAGAACAATATTTGCATTTGAAATTGGAAGATTATTTTTTG

[1485] CACAAGTGCCTATATGCTATATAGAGTTTGCCCACTCTGCATTATCTTCCCCCTGTTCC(SEQ IDNO.729)

[1486] EGFRvIIIDprobe0730:CCCGTTATCTGGCACAAGCTATTCAAAAGACACGCCTACTTGTAAAATAAATGGTTTGCA

[1487] AACTAAGGAAAATACTTAAATCTCATGTAAATGGTACTATACTATGTATAAAAATGTGAA(SEQ IDNO.730)

[1488] EGFRvIIIDprobe0731:GAAACACAGAACAGCTCATGAACACCTCCACTGCTGTATAAAAGAACCATCTTTTTTCTG

[1489] GCTCCTATTGGATGCCTTAGAAAAATCTGTATTTCCTCTTTAGTTATTGTGTTTGAAAGA(SEQ IDNO.731)

[1490] EGFRvIIIDprobe0732:TGAAGTTGAGACAAAAGTTCTATTCTTTTTAAGTTGGCAGAACTTCTGAAAGGTGATTTT

[1491] TAGCTGCAGTGTGACTCATTCCAAATGCAGAAATCTCTGACCCTGAGTTAGTCTATTTGT(SEQ IDNO.732)

[1492] EGFRvIIIDprobe0733:CATGCAAGAGCCTAGAAAAGCCCTGAGTGATAAGAAATGGCCATAGGCCATTCCCACAG

[1493] AATTTTCAACAAAAATAGAATCATGCTTATGTTCTAGTCATGACTTAGAACTTATAACTCA(SEQ IDNO.733)

[1494] EGFRvIIIDprobe0734:TGTTCGGAACTGTCCATGTTCACGCACAGGGGCCGTATCACTCCGCCAGAGCTGCCCTGG

[1495] GTGCCGGTGTGCAGAGGGGTCCGAGAGTGACTGTCTCTTCCTCTGTTGTCGAATGTGTGG(SEQ IDNO.734)

[1496] EGFRvIIIDprobe0735:GTTATCTCCATAAATGGCTGCCATGAGCATCCTTGTTCACACATTTTTAGGTACTTGAGTG

[1497] AGTGTCTGTGGAATAATTTTGGGAAGTGAAATCTGTGGTCAGAGGTTTGTGAGTTTTAC(SEQ IDNO.735)

[1498] EGFRvIIIDprobe0736:ATGCTACATTTTCAGAAGTTGAGAAATAGCAGTAGGCTGAAGGCAAGTCGCCATGCCTGG

[1499] AATTCATGAACACTAGTTGAAAGAACTGGCGTGAGTTAGTCATGACAGGAGAGATGGGGA(SEQ IDNO.736)

[1500] EGFRvIIIDprobe0737:AGGGAGTTGCAGGTAGGAGGGCCATCTTCAAATTCTCAAAGTATAGTCACTCCAAACCA

[1501] AAATTCGATTTAATCTGTAGGACTCCATTCTCAAAGCACAGTCACTCCAAACCgaaattcg(SEQ IDNO.737)

[1502] EGFRvIIIDprobe0738:atttaatctgtaggactccaggtggcagaataagaggcaatggatgggtggaagcgaaacagggccaaagtttgacttcatgtgcaacttcctaa

[1503] ggagtgatttgaactccacaaacat(SEQ ID NO.738)

[1504] EGFRvIIIDprobe0739:gaactaagcACCTCAACACAGGCTGGGCaagttgctgttcttttggagcttacatcttagtggggaaagagaaatgcctatgtaaa

[1505] catataaatcagcaggatacattgtGAGGACGGT(SEQ ID NO.739)

[1506] EGFRvIIIDprobe0740:CATTGCTCAGTGAGACTGCAATAGAGTGATACGCTGGAGGGGGCTGCAAGGGAGAAGGT

[1507] GGGAGGGACAGCATTTAGCAGAATGAGCAGCACAGTCCCATAGGAAGAAGAATTTATTGCC(SEQ IDNO.740)

[1508] EGFRvIIIDprobe0741:TCCTTAGGCAAATAAATTCCCAAACCTTGAACATCAGAAAGGAAATAGATTAATGTGCAC

[1509] AGAGGATTAAATTATGTGATCTGCAAAGTCATTTAAAATCTATTTCCACATAAAACATAT(SEQ IDNO.741)

[1510] EGFRvIIIDprobe0742:TAATGCAACCTAAACAAAAGGGGTCTGGATACCCTCATCTTCTTCCCAAGCATCAAGTCT

[1511] TTCTATAGTTAAACTGAGATGCTTTTATTCTTGGAAAATTTTAAGGACTATCTACAGCAA(SEQ IDNO.742)

[1512] EGFRvIIIDprobe0743:TGGAAGAATCGGGTGTTGGGATGTGTTCCCAGGTAATAATGACTGCAGGCTGATTTGGCC

[1513] CTTGAGGTGTGGCCTCATGGCCCTCTCCAAAAAAAATCAAGGACCTGCTACAAAGCACAA(SEQ IDNO.743)

[1514] EGFRvIIIDprobe0744:AGCCGACTGCAATGCTTGCTGCTTACTGGTTAGGGCAGCTCCTCTTTGCCAGCGACCAAG

[1515] CAGAAAGCAAGACAAGACAGGTTCTGAAGCAGTAATTCAAAGCCTTCCTCGCTTTCCCAT(SEQ IDNO.744)

[1516] EGFRvIIIDprobe0745:GTGAGTCATTGCTAGTCAGAATATTACCTTTGCAGAGAGGCTTAATTCCAAATTTGCTCTT

[1517] AAAGGGATATCCTCTCCTGGTTTAGGTATAAACTTTTGACTCACAGGACAAATTCTATC(SEQ IDNO.745)

[1518] EGFRvIIIDprobe0746:ATTCCTTTGGGCCTAGGATTGCATTTATTTCCATGACAAAAGGGCCTGTCTGGTGTTTCAG

[1519] CAAATGAAAACAAAAATATAAAGCCCATCTCCTTTTGAATGAGCTCTAAAACAGTTCTC(SEQ IDNO.746)

[1520] EGFRvIIIDprobe0747:CACTGGACTTCAGAACAAGAGGGAGCTCTGGGCTGCTGGCTGGTTGTGCATTTGCTGTG

[1521] GGTTCCCTCCGGCAGGCGACCTCTCCGCGCTGAGAAGGTTATCCGGATAACCAAGTAAGAA(SEQ IDNO.747)

[1522] EGFRvIIIDprobe0748:AGTACATGAGGAGGCACAGAAAGAAAAATGTGAGAGATAACAGCATAAACACACAGTG

[1523] TATGTTGTTATGAGGCATCACATGATGAGATACTGCTGGGGAGGGAAGAAGTGAGGAGATTC(SEQ IDNO.748)

[1524] EGFRvIIIDprobe0749:CTAGGAATCTTATGAGAATTTCCAGAGACAACAAGTTTTGAGCTTTTTTTTAATTTAGAAA

[1525] ATTTACCTTATTTTTAAAAGAATATGTAACATATCCCATGCTATAAAATTCTAGACATA(SEQ IDNO.749)

[1526] EGFRvIIIDprobe0750:GTAGATTTAAAACAGCATAATGGAAAATATAAATATCTATTTTCTTTTCCTATTTATGTATTC

[1527] TGTGCCAGTAGGAATGTAGCCAAAAAGAGAGAAAAGGGGTCTCTGCAGACATGGATG(SEQ IDNO.750)

[1528] EGFRvIIIDprobe0751:TCTCTGTGACTTGATCACTGCTAACCCAAGAAGATAATAAAGCAGAAGCATGTATCCAGG

[1529] TTGCTGCAGCCAAGCCTGCCCGGTCTGCGGGGCGTCCTCACACATGGGGCAGCTCTCCCA(SEQ IDNO.751)

[1530] EGFRvIIIDprobe0752:CCCCACACACTGGGAAAGGCGGACAGAGGCTGGGCAAAGCCCCCAATTTTCGTTGGCA

[1531] CTGACCCCGATGATTTATAGGCCTTTGTTTCCCATGTTAAATGTCTTACGATCATTAAATTA(SEQ IDNO.752)

[1532] EGFRvIIIDprobe0753:TTTATAGCTCAATTAGCATGTGTCCAAAACCAGGAAGTTCATAGGAGACTGTGTGACTGG

[1533] GAATTAAGGAGCAAAGCAACTTTCCAGTCTGTGATTTACTGGGTTTCCATTCTGTTTCCT(SEQ IDNO.753)

[1534] EGFRvIIIDprobe0754:GTTCGGATCCGGAAGTAGAATTTCAAATATTGCTTTTCATGCTTTATTTGGGACCGATTTTA

[1535] GCCCCGCTCTCCTTTCTCTTGCCATTCGCTGGCCATTAGCCACCAGCCTCTGCACAAT(SEQ IDNO.754)

[1536] EGFRvIIIDprobe0755:GACCAGCTGGCCCCTGGCAGATCTTGGGCCCAGGTGTGAAGTCGCTGGAGAAGCATTTC

[1537] AGGGCCAAGATGGGAGTGATTTCATTTTCCATTGACACTATGCAGAAATGAAGGGGATTCA(SEQ IDNO.755)

[1538] EGFRvIIIDprobe0756:AGTGCCTTCAGAAAAGCTTCCTTCCAGCGAATGGAGTTTTGGGGGTTTTCCAGACTTGCA

[1539] ACTGCTTTTATTCTTGGAAGCATCATTGTTGCTTTTTCCCCCCTTCCATTTATATCCCAG(SEQ IDNO.756)

[1540] EGFRvIIIDprobe0757:GAACTGATTCAGAAACCATAGAAATTGGATTTGGAATCGCTGAATGCTAGCAGACAGCTG

[1541] ACTGCACTCTTCCCAAGAAACCCTGCCAGCTGGGTTCGGGTATCGCGCGGTGTGTGCTCT(SEQ IDNO.757)

[1542] EGFRvIIIDprobe0758:CTCTGCCTGGCCCGCTGAGTCCTCTAACTCTAATGGATTCCTTCTTACACCAAAGTGCACT

[1543] AGAACTAAAGTGTTTTGCTTCATTCTTTAGACATTTTGTGGTTTAGGGCTCAATCAGCC(SEQ IDNO.758)

[1544] EGFRvIIIDprobe0759:AGGGTATGATTTGCAATCCACAGTAACCGGTTTCAGAGCAGCTGCCCAGCGAGGCAGGT

[1545] TTCATCTCGCTTGCTAGACGTTTTGTTTTTTTTTTTTTCTAAACCTCACACCTTTTATTTA(SEQ IDNO.759)

[1546] EGFRvIIIDprobe0760:TTAGACTTGGATTCCAGTTTCCTGAGCCTGTTTGTGCCACTGATTAGACAGGCTTGAAGC

[1547] AGAACCCACCAGGCTTCCTGAATAAAATGCAGCAGTGATTGTATTAGGGGGTTTTAAATT(SEQ IDNO.760)

[1548] EGFRvIIIDprobe0761:GCTCAAAATACTGTCTAAAAAACACTAAAAATCATGTTACTTTCTAGATTGAATAAAATCC

[1549] TATAGAAATGAATTCCTGGACTTGATATGTAGCAAGCTGGCATTGGCTCGGGAGTGAGT(SEQ IDNO.761)

[1550] EGFRvIIIDprobe0762:GGGCTCAGTTAAGTGAGCTAAGATGAGATGGTGCACAGGCGAGCACCCACCTGAGGAGT

[1551] GTTTGGATGTTATGATAGCCAGCTCCTCTGTAAAGACCTGTCCTTCTATGTCAGCAGCCCA(SEQ IDNO.762)

[1552] EGFRvIIIDprobe0763:GCAGATAAATGACGTGTAAATACCACATTTAGGAGGGCTTATGATGATGCCAATTAATGGA

[1553] GACCTTTTTGAAACAGGAAGGAGGTGAAACATATTCCTTTGCTTCTACATCACTGTGTG(SEQ IDNO.763)

[1554] EGFRvIIIDprobe0764:CCAGGCACTGTTTACAGCATCTCGTTTAACCAGCAGTCACCACCTGACGGATGGCTGATG

[1555] TGGGGTGGGGTCCCAGGGTGGGATTGCGTGATGGGCTTGGGGTCTCTGGCTGATGGGTGC(SEQ IDNO.764)

[1556] EGFRvIIIDprobe0765:CAGAGCTGGGACTGGAACTCCTGGCGTGACTGAGGCAGACACCTGGGCTACCCAGCCTC

[1557] ACCCACGACGCCCTCACTAAGTGACCCACAGGACTCACCGGAAGCAGGGCAGCAAGGTCCC(SEQ IDNO.765)

[1558] EGFRvIIIDprobe0766:CCTACAGAGGTCCCCACTGCAAACCGATACCCAGCTTAGACAGCAGTTCTGCAGTCGGC

[1559] GTCTCACCCCTTCGGGTCTCATTGTGACTCACTTTGATAGCCACACGATTTAAGGGTGGTT(SEQ IDNO.766)

[1560] EGFRvIIIDprobe0767:CAGTAGTGATTTGATGAGTGCTGTGGCTCAGGGTCATTCCCCTGCCCAAGCATTTCAAAT

[1561] TCCAGAAGTTCATGCCCTGCATGGTGGGTGAAAAGTCTCAGGCCAACCATGAGCACACAG(SEQ IDNO.767)

[1562] EGFRvIIIDprobe0768:CAGCCAGGCGACTGAGGCAGCTGCCCGGGGTGGCACGTTGCTCAAACCCATCATTTGGA

[1563] GTCAAAACAAACAGATGATTAGCTGGGGTGGTCACTTTCAATCAAGAGTTTTCACATCGCC(SEQ IDNO.768)

[1564] EGFRvIIIDprobe0769:TAGACATGGCCTCAGAATCAGGCCTGGTGTGGCCAGGGGCTGATCTCACAGTAGACAGG

[1565] AAGTGTGGCCCGAGGGCCATGGCTGCCCCCTCAGAAGGCCCTGTGGAGTGGCTGGCCGAGC(SEQ IDNO.769)

[1566] EGFRvIIIDprobe0770:CTCAGCAGCCTCCTGTGAAGCGAGGAAGGGTCTTCCTGCCGGCCTCTGGAGATCAGTAT

[1567] GGGAATGCACAAGTAGGAAACGCTGGATGGGAATCCCTCTGCCCTGTGATACCAAGGCAGT(SEQ IDNO.770)

[1568] EGFRvIIIDprobe0771:GAGtttgtagactatggaattgctgtcggagggctctgtaaccggccaaggtcacacaggtagccattggtagagcagggactggaatcccag

[1569] acccccaacttccaggactgtgcacct(SEQ ID NO.771)

[1570] EGFRvIIIDprobe0772:ttctttatcccatacagcctTACAGTCAAGTGCCAGTGCAACACCTGATTCCCAGGTTCCAGCCTTTG

[1571] TCTTTTATAATGGGAATCAACCTTATCTTGACGATCCAGAGATAGTCATCAA(SEQ ID NO.772)

[1572] EGFRvIIIDprobe0773:GGAAGATTAAATTATCCCCTTAGACTCagagtgaccatatcattttccctccacacaaggacacttttgagaatgaaaagg

[1573] agGAGATGTCTGTACCAGACGCTGGATGACAGGCACCGA(SEQ ID NO.773)

[1574] EGFRvIIIDprobe0774:CAGGCTGTCTGCCAGGGGAGCAGCGATTCCTGTATGTTGTAGAAAGTTTTTCAAAAGTCA

[1575] CCTTGGAAAGAGGTTTTGTTCCTTAACCTTCTGTTAAATAGGAAGCTCCGTGAATGAAAA(SEQ IDNO.774)

[1576] EGFRvIIIDprobe0775:CAACTCCCTTCCCTAAACATTCTAGTAATGACCCAACACTGCCAAGCCTGCCAGCTCTGC

[1577] CTCATGGTCGTGTTGACTGTGTGAGACTATGTGAGTGCCTGCTACACAGTACGCTTTCAG(SEQ IDNO.775)

[1578] EGFRvIIIDprobe0776:TAAACATGGTATTGCCTCGATAATCCCACAAAAATGTCCTATTCAAATCACCTGGCACCCA

[1579] GGAAATTTCCTTCTTTTTTTTCCCAGGTGAAATATACAGTTGAAAACACCTGACAGCAA(SEQ IDNO.776)

[1580] EGFRvIIIDprobe0777:TTCCCCTCTCCCATGTGTTTGCAGGATGGTGGTTTTGGTTCCTCCATCTTTGATGTGTACA

[1581] AGTGTGATGTTTTCCCCCCACAGACAAGTAAACCACATTCTCTTCACATTCCCAATGTT(SEQ IDNO.777)

[1582] EGFRvIIIDprobe0778:TTGTCAATGTACCTCCTTCAATAGAGGATCGATAAGGAAAAAAATCATTGACAATCTCAAT

[1583] TAGATTCACTATTTCATCCAAAAGCATAGCTTAGAACTCTAGTTTTTGTTCAACACTCT(SEQ IDNO.778)

[1584] EGFRvIIIDprobe0779:TGCCCTATGAGTGCACAGAACTTTAATTCTGATACAAACATCCCTGAATGTTTAGCTTTGA

[1585] CAGAGATTCCAAGGTGATTTGATAAGAAGCAGGGCTGTGTTTGGGCTCTGGGAGTTTTT(SEQ IDNO.779)

[1586] EGFRvIIIDprobe0780:GATATGGTTTCAAGCCCCATCCAAAACCCACAGACCTCTAGAAAGTAGGTGCCTGCCTTC

[1587] CTGCAGCAGCCCTGGAGCCTGCTGGGGGCTTTGAGCAGCTGCTGCCAAGCCAGGCCTCAC(SEQ IDNO.780)

[1588] EGFRvIIIDprobe0781:CCGACACTCTGATGGGCACGGCCATGGTGGCAGGGGCTTGGACGCTGCCAGGTGACTCT

[1589] AACTTGTGGCCAGGGTGGGAAGCACTGCTCCACAGAGGTGCCAAAACCAGGTTCCTTCCTG(SEQ IDNO.781)

[1590] EGFRvIIIDprobe0782:TGTTCTCACATTTCACAGCCTCAATGTAAAAAGTAAGACATGGGCACTCTGGAATATTAC

[1591] AAAAATATAGAAAAGCATGTTATAGTAAATAAAAGGCTCACAGAATTTTGTCATTTAGGA(SEQ IDNO.782)

[1592] EGFRvIIIDprobe0783:ACAATGATTATTAATATATTAGTGTGTGTTTTTGCTCATTAACAGTATATCCTGAGATATTTC

[1593] CTATACCATTTAATATTTTAAAAGATGTTTACActggccacagtagctcatacctat(SEQ IDNO.783)

[1594] EGFRvIIIDprobe0784:ATATTTTAAAAGATGTTTACActggccacagtagctcatacctataatcccaacactttagagggcaaggcaggaggatcactt

[1595] gaggcttaaaaattagccaggtgtagtggcacatgc(SEQ ID NO.784)

[1596] EGFRvIIIDprobe0785:cagtgagctataattgcaccattgcactccagcctaggtgacacagtgagaccctgtttctaaaataaataataaataaaTTAAAACATTT

[1597] AAAAATACATGATGTTTAATTATTAGAGG(SEQ ID NO.785)

[1598] EGFRvIIIDprobe0786:TGATGTTTAATTATTAGAGGACTCAATTTTATATCTATGTATACAATAATTTTTAAGTTTCTTA

[1599] ATATTGGACTTTTAGTACCtttttaaaaatactatttttaaaaaaatCTGTATTTC(SEQ IDNO.786)

[1600] EGFRvIIIDprobe0787:TAACTTTTTATAACAAGGAACCTTTGGCTTTGAGATGACTGGGGAATCCATTCTTTCCTAT

[1601] AGTATCCATGTCCAATGGACTTAAAGTATTAATCAATGTGTTTATGTTTTGTTATTTTT(SEQ IDNO.787)

[1602] EGFRvIIIDprobe0788:CTGGCATTACAAAAAATTCTAAATATATTGTTACCGCCTGTATAAATATCAGCTTTTGAGAG

[1603] AAGGACATTGTGTAGAAATAATGAAACACTGCAACTTGTATTTGTATTAttctttttt(SEQ IDNO.788)

[1604] EGFRvIIIDprobe0789:tgatctgcccgcctcagcctcccaatgcactgggattacaggcatTATATTATTCTTTAAATTCACATGAGAATTTAGT

[1605] ATGGCTTCAAAAAATACCATAAGTTAAAATATCACCAAGAC(SEQ ID NO.789)

[1606] EGFRvIIIDprobe0790:cccaatgcactgggattacaggcatTATATTATTCTTTAAATTCACATGAGAATTTAGTATGGCTTCAAAA

[1607] AATACCATAAGTTAAAATATCACCAAGACTCTGTTCAGACAAAAGTATC(SEQ ID NO.790)

[1608] EGFRvIIIDprobe0791:AGAAAAGTGAGCCAGGCACTCACATAGTTTATAGTTTATAAAAGTGAGACAGGCATGATC

[1609] TCTTAACCTCACTATAGTCCTGTGAATAAGGTTTATTTACATTTCATTTTACCTGCCAGG(SEQ IDNO.791)

[1610] EGFRvIIIDprobe0792:ATTATTGTAAAAACGCCAAGCACATTGCCTACACAAACTAAATATTCAGTCAATGGCTGCT

[1611] ATTTTCATGAGTTCGTTTTAACATATATTTATTGTCCTCTACTGGATTTAAGAAGTTAT(SEQ IDNO.792)

[1612] EGFRvIIIDprobe0793:ATTTATTATCATCTAAGATTTTAGCTATTCCTTCTCTTAAAAATAGATTTTATAATCAATGGC

[1613] AGTAAGGGAGAGTAACTCGCAGTTCTCTGAATCTCAAGGGGTTCCTGGAAGCCTTCC(SEQ IDNO.793)

[1614] EGFRvIIIDprobe0794:TGAAGGTATAGTGAAATTTCAGCTTCACATTCCCATCCATGAGCTCCCTGCAAATATCCCG

[1615] GTCTGCTCTCAGGACCCAGTGACTTACCTATGCAGAGGCTGTAGATAGCACCTGGAGCT(SEQ IDNO.794)

[1616] EGFRvIIIDprobe0795:TCCTGTGTGCCCTCCTCAAACTCAGCCAATGCCGTCATACAGTAGCAGGCAGGTGTCTTT

[1617] GCTGGGTAGTTGGACTGGATGTCCCTGGGATTGCAGAACTGGAATGGGGAGTGACATCAG(SEQ IDNO.795)

[1618] EGFRvIIIDprobe0796:GAAACTATAATCATCAGGACAACATGGTTTGCCATAACTTTAAGTTTTAAGCGACCGCAG

[1619] ATTATGCGGAGAGAGATGCATGCCCACAGCCATGCTTCCCATGTAACTGGAGAGGGGTCT(SEQ IDNO.796)

[1620] EGFRvIIIDprobe0797:GAAGTTTGAAACAAGTGTTCCTAGGCACGGGTTACAGTGTTTGTTATCATCATACTTGATT

[1621] TAGAATGGGGCACAACATGTGGATTCATGGTAACTGTTACAACCTTACTCATTTTAATA(SEQ IDNO.797)

[1622] EGFRvIIIDprobe0798:CCTGAAAACATGCTTTCCCCATGCTGGGAATCGAAAGATTCTCCTAGGAAAAGAAAGGC

[1623] TTGACAACATCGATTCAAAAAGGGCATGCATTTTCCTCATTTAAATAACTCTAATGTGCAA(SEQ IDNO.798)

[1624] EGFRvIIIDprobe0799:GTAGATCCCCTGACCTCAAGCTCAGAAGAGTCCAGGCCTTCACACCTTCTCTGCTTCTGC

[1625] TCTGGGGCCAGCTATTGAGATTCCTGTGCCCACGCAATGCGCACATCCCACCCCTGGCCG(SEQ IDNO.799)

[1626] EGFRvIIIDprobe0800:CTGTCCACAAGAAATCCAGTTGCACCAAGCACCCCACTTTTTGCACCTCTCATTTATGTA

[1627] CTCCTAAGAGCCTCACCACAACTCCCTTCTAAAAACATGAGTTCCTGACTGGGAATTCGA(SEQ IDNO.800)

[1628] EGFRvIIIDprobe0801:TGCTGCCCAGGCAGCTTTGCTCAGAGGGAGCAGCCTTCTAGAAATGTTTCAAGtaaactttcaa

[1629] gtataactaaattcaaaaaaaacacatacacacacacacacacacacaagtcaaag(SEQ IDNO.801)

[1630] EGFRvIIIDprobe0802:gtgtgtaatttggccaatatcacaaaccaattagccctttgtaagtggcacccagatcaggacagctgaccataccagcaccctagaagcacccc

[1631] gtgctgcctcctgggacagggctac(SEQ ID NO.802)

[1632] EGFRvIIIDprobe0803:caccatcctaaggccagcacgatgggccagctttgcctgctgttgaattttgcttacatagaatcctccagtaggtactcctttgggtcaggttctttca

[1633] ctcaacattatgtgttgata(SEQ ID NO.803)

[1634] EGFRvIIIDprobe0804:tttttccatgctgtgctgcaaaattgtatttcttgcattccataactgggcagttccatcataggagaataccacactgcgttcgtccattctaccgccaa

[1635] tggacatatgggttctttc(SEQ ID NO.804)

[1636] EGFRvIIIDprobe0805:tcttttcttgcagttacaagtttatgaatattgtcccacgtgtccctggtgaacttttgtttgcatttctgttgggtacctcagagtggcgttgctgggtcag

[1637] agggtactggtcgcttt(SEQ ID NO.805)

[1638] EGFRvIIIDprobe0806:agtagctttgaaagatattgccaaaacattttccagcgcagttatagcaaattatacaccaccagcagtAGAAAACATCTCCTAATT

[1639] GCTCACAGTAAACCCCCAAAGATTGCCACATAC(SEQ ID NO.806)

[1640] EGFRvIIIDprobe0807:ATCTTCCATATCAATTACTTAACTATTCAGCAAATTTGAAGGGAAATATATTTAATCTTTTTA

[1641] TTCAAATAGTTTATAAAGTGGAATAGAGATGTGGGTAAAAGTTGTCTTGCCACCTTT(SEQ IDNO.807)

[1642] EGFRvIIIDprobe0808:TTAGATCGGTAAAAGTTTGTTGAATGCAGGCAAGAAAAGATGAGAAATAATGGTACCCAA

[1643] TGAAAGACATAGCAGTCTACAAGGAGGGGCATTTCCCGGGGTGGGGGGGACCCACACTCT(SEQ IDNO.808)

[1644] EGFRvIIIDprobe0809:GTAACTCCCACATTCAATTAGCATGTTATAGGTAAGCTGCAGAAAACGAGGCAGCTTGTC

[1645] AAAGAGGAACGGCTCTTGGCCATGGTTGCTGCCCTAGGAGGATATTTGATACTAGCAGAG(SEQ IDNO.809)

[1646] EGFRvIIIDprobe0810:CTGGGGCAACCCTGGAGGAAACCACCTGGAATGATGGGAGAACTCCTCCAGGGAACAT

[1647] GGCCCTTTAATAGATCTCTGTTATAAAAAATAATCCCAAAGCAGCCACCAGGGCATACTGCT(SEQ IDNO.810)

[1648] EGFRvIIIDprobe0811:GCGATCAAGTCCTAGGCGGTATTCCCTTCTGCGCCATAGACCCTGTGCAGAGTGCCCTCA

[1649] ACGAAGGAGCAAGGAAGACCAAGTCTCCCGAGGGTTTGCATATGTGTATGTGATTCTGCA(SEQ IDNO.811)

[1650] EGFRvIIIDprobe0812:GTCATGGTGAATGACACAGTCAGGGCTGCGGAAAAGCATTGGTAAAGTGTATATTTGAGG

[1651] CTTCAGAAGTTTGAAAAGGCTAGATTTCCTAGGCCAAAACACTGAAAATTTGCAATTAGA(SEQ IDNO.812)

[1652] EGFRvIIIDprobe0813:ACTTCAGTGCTGATGCTGGGAAGACTGGAGTTAGTTTGAGACATGCACCTGTGCAGAAC

[1653] TGGGCCCCCAGAAAAGGAGAAGGAAGGGAATCCAGACCAGAGTAGGGCCTGACACCACTCA(SEQ IDNO.813)

[1654] EGFRvIIIDprobe0814:GACTCGGCGTGTCTATAAATTAGAATTGCGTTACAATTACACTTTGACATTTTAGTGGTTTT

[1655] TAAAGTGCCCAGCACAAGTTAATTTTTCATTAATGAATCCTTTATTCATAAAATGCTT(SEQ IDNO.814)

[1656] EGFRvIIIDprobe0815:AGATGGAGATTACCCTTTTGAGCATTTTGCCAGTGCTTCTGAAATTAATGGGGACCTCCTG

[1657] TTGGAGGACACAGTCTGTTGCAATAGGTGACCACTGCTCTGAATCTATGTCACCTCTCC(SEQ IDNO.815)

[1658] EGFRvIIIDprobe0816:Aggaccacgggcacaaccatcacctgaggcatgttggagatgcagatggtcaggccctcctagaatctcagaatctgcattttagcaaagtcctg

[1659] ggtaattcctatgtccattggagtt(SEQ ID NO.816)

[1660] EGFRvIIIDprobe0817:tgagaagcactgGTAATCTCAAATACTTTAAAAGATTACTAGAGTAAGATAGGCTCAGTAGGTAC

[1661] CTGAAGGCACCATCCCAAAGACCAGAGTGGTAGAAGCAGGTGGACCAGCCTCTGA(SEQ IDNO.817)

[1662] EGFRvIIIDprobe0818:ACACATTTCTCCCCCACTCCCCGGCTGTGTGGAAGGTTGCCACCTTTGGGGTAGtcattcaaca

[1663] aacacgtgtcaactgtccactatgtgtcaggccaccactgggcactggctgtggct(SEQ IDNO.818)

[1664] EGFRvIIIDprobe0819:agctggatagacaccatttctgccctccagaaatgtcatgtccactggcacatgacaagtcactaagtcattcagagccatgggtgacagctccag

[1665] gggccgacaaaggagCTGTGATCT(SEQ ID NO.819)

[1666] EGFRvIIIDprobe0820:CACAGATCCACAGAGAAGTGTCCCAGGGCGGGCGGGAACCAGGACTGCACAGGGAGGG

[1667] GTGAAGTGACACATAAGAAGTCAGCCCATCAGCCTGAAATGCTCCCCCAAATCTTCCCATTC(SEQ IDNO.820)

[1668] EGFRvIIIDprobe0821:AGTGTTTTCTCAGTAGCAAACTCGTGGGAAAATTGGTTATTTTACTTAAAAAACTCATACT

[1669] AGAAAGCTAGTTTAACtttaaaaataaattttaaaaacatttttattaaCAAATCCTAC(SEQ IDNO.821)

[1670] EGFRvIIIDprobe0822:CTTTCCTCCAAAGTCAAGGAGAAAAGAATAGAAGTGAACAATGGACCAAGTAAGCCTAA

[1671] AACTCTGCTCTTTCCCCTGCTCATTTTACAGTTCAAGTGCCATTCAATTTATCCTGGCAAG(SEQ IDNO.822)

[1672] EGFRvIIIDprobe0823:AAGAGGAAGGCATCATCAAGACCTTAATTTTCTAATACATCTGATCTGAGAAGAATGTGA

[1673] AAGCTATAAAATTAATTTTTGATCAATAACTACAGGCCTTTTGAGAGAGTGCCCTCCTAA(SEQ IDNO.823)

[1674] EGFRvIIIDprobe0824:TGAATTGAGTACCTATTTCTCCATACACAGTGTCTATCATGACCTACAAACCCTTTTCCCAT

[1675] GAGGTGTAACAGAGAGAGATTACAGCCTTGGAACTGGATGTCAGACTCTCCTGGTTTA(SEQ IDNO.824)

[1676] EGFRvIIIDprobe0825:AGACAATAAGCCATGACATAGAGCCTGAAACCAACACAATCTTCCGAGTGGTTCCAGAA

[1677] ACATATAGGGGATAATGTTGGCTCTGATGCTGTACATCCCCAACAACCATCAACTATTTGG(SEQ IDNO.825)

[1678] EGFRvIIIDprobe0826:AAACTAGAATTTCAGCATAATTGGAGTTGGTGTTACCCTAGCAAATGCTGTGGGAAGAGA

[1679] GTCTCACTGTGTATCTTCTCCTGTTTAAAGCCTGAATTTGTTCAGAATGTAATATCTCTG(SEQ IDNO.826)

[1680] EGFRvIIIDprobe0827:TTTAGCCACTCTACTGAAACTGATCTAGGAAATGTTCAAAAAAAGGTATCCCAAGGATCC

[1681] CTTTGTAGCTACATCTGTGGGATTCCCCTCGCTCTGGCGTGGCCTGGCCCCTCTGCATTT(SEQ IDNO.827)

[1682] EGFRvIIIDprobe0828:GACAATACGGTCCTATGCTTTTGTCTTCCTGGGCTGCGTGAACCCACCCTGCCCTGGTTC

[1683] ACCTCTCCTCTTGACCCATCCTTATCAGTGTCTTGAAAGGTCCTTCTATTGGAGGACACA(SEQ IDNO.828)

[1684] EGFRvIIIDprobe0829:TTCTGTTGCAGCAGGTGACCACTGCCCCAAATCTGTTTCACCTCCCCAgggccatgggcacaacca

[1685] tccctggagtgtgttagagatgcagttggccaggtcctccaaaatctcagaatc(SEQ ID NO.829)

[1686] EGFRvIIIDprobe0830:tgcatttttgcaaagtcctgggtaactcctatgtccatgagagtttgagaagtactgGTCTCATGAGTTCCTGACATACAAAT

[1687] AGTGCTGAGGCCAGTATGCTGACTGGGTAGCCAGATA(SEQ ID NO.830)

[1688] EGFRvIIIDprobe0831:CAAGTGAAAACCTTCCTGTTTTTTGCAAACCTGGATGGACCCGAGGCCGCTGACGTGGG

[1689] CCAGGACAAGCTACTCTTTTTCAGTGTTTCTGTTGCATCGCTGTGTCTCTCTGTGATCAGG(SEQ IDNO.831)

[1690] EGFRvIIIDprobe0832:TGCTGCCCTCCCTGGCAGGAGGACTGCAGACAGGATGACCAAGAGCACTCTACACAGCC

[1691] TGCTCTCCAGTGTTGGGGGACGCCACCCACCCTCGTGGTTCCTGTTCATCTGCCTACACGT(SEQ IDNO.832)

[1692] EGFRvIIIDprobe0833:GGAGGGCCCAAGAGGGCTAATATGTGACTATCTCCACTTCCTGGTACCCTGTGTGAATAA

[1693] CTTCACTTACTAAAGGGATGTTGAGCAACTTTATTAATAATGAAGAAAGCACTTTGGTTT(SEQ IDNO.833)

[1694] EGFRvIIIDprobe0834:GACAAATAATCACTCCATTTTTTCATTTGAAAGTTAACTCTTGTTAGTAGAGAAAGCAATG

[1695] TATTACAACCACAAGGACGTTTACATGGAAATGAACCATCTGCAAAGCATCCCCCATTT(SEQ IDNO.834)

[1696] EGFRvIIIDprobe0835:TCCTTTTAAATCAGCCAATGGGTGGTGGTGGGAGAAATATTCACCAGAGtatttaacatctatccccct

[1697] tcctagactgtcagctccatccgggcggagactgttggtatctccacagca(SEQ ID NO.835)

[1698] EGFRvIIIDprobe0836:cacacagggcctggcacacatccggggctcagtgagcacttgctgaatgGTGAACAGATTAGCTCTCCTGGGAACGT

[1699] TGTTGACACATCTCATAACACTGGTTTGGAGTGGAGGGCATTC(SEQ ID NO.836)

[1700] EGFRvIIIDprobe0837:ATCGGGCTGCATATTCCTATTTTTAATTGTATTCTCCACTGGTTACAGCACCTACAGTTATA

[1701] AAGACATTGTTAACATTGCTTATAGGAAGACATTTGATGGAAATGAGTCCAAAGGCAT(SEQ IDNO.837)

[1702] EGFRvIIIDprobe0838:TACGGTTAGAAACTGGCCAGGTGTCATTTTTGAGAGATTAGATAACTGTTTTCCGGTAGA

[1703] GTGAATTGCCTGTTTGTTGCAAGTTGGGACTTTGCTGGGCTGGTTTACAGGGCCAAGGGG(SEQ IDNO.838)

[1704] EGFRvIIIDprobe0839:AAAGAGATAAGTGGATCTTCTAGTGAGAGGTCATCTGTTTTGAAAGCCTGGAAGATTCCA

[1705] TGAACTAAATCCAAGTCTTACAACACAGGGAAGTGTGTCATACTGTGCAGGGATGAAGTC(SEQ IDNO.839)

[1706] EGFRvIIIDprobe0840:TCCAATTTAGCATGAAAACAAGAGCTCCTCACACTGTCCTCTTCAGAAAGCCCATACAAT

[1707] CCAAACTTCTGAATGCTTAGCTGCTTACAACCATACATAGATTGAGGGATAAAACTCTGA(SEQ IDNO.840)

[1708] EGFRvIIIDprobe0841:TATGGAAGAGAAGGTAAACATTTTTTGGCAGACATTCCCAGGAAAAGGCGGCTCTCTTCT

[1709] CTCATTGCTGCTGCTCTTTCAGAATCCATTTCAACAGAGGAGGAGTCAATGGGAGCCCCG(SEQ IDNO.841)

[1710] EGFRvIIIDprobe0842:TGCCTCTGGCAGATATCATATGGCGTTTCAGTGGCATTGTGTGTTACCCTTCTTAGGTAAC

[1711] AGCTCAGCCATTAGAAGAATGTCCTACACACCTTCTCATTTTCTGTGATGAGAGGAATG(SEQ IDNO.842)

[1712] EGFRvIIIDprobe0843:TGAGGTACTGCCCTTCGAGAGCTGTCATTTGTCCTAGTAGCCAGCAGCGTGACTGTGCTG

[1713] TCTTCTGCTCTGTCTCCCTGTCAGCCTTCTGCCCAGCCACCACCACTATAGTTTTGTTCT(SEQ IDNO.843)

[1714] EGFRvIIIDprobe0844:CTCCATTGGAACTCCTGGTTCAGAGAATTACCATAAAAAACAGACCCCTAGACATACAAC

[1715] ACTCTATCACATAATGGTGACTTTGTCTTCTATTTTGGATTACTGAGCTTTCTTGGGTAA(SEQ IDNO.844)

[1716] EGFRvIIIDprobe0845:CTTCCACTAAATCGAAGTTAATATTAGAAGAACTTCCTCTTACTAGAATCGAAAAGCATTT

[1717] AAGTGATGCAGTCAAGTTTGTACCATAAGTAATTCAGTCATTTAACAAATATATATGGC(SEQ IDNO.845)

[1718] EGFRvIIIDprobe0846:CTCTGTGCGACAGTGACCTTGACTGGGAATGAAGCTGTCCCATGTGGGGCCTGTTCTTCA

[1719] AAGGCAGTTCCCTGCTGCCCAGTTCAGTCCAGTGGATCTGGGCATCTCTCTTTAATCCGC(SEQ IDNO.846)

[1720] EGFRvIIIDprobe0847:ATTAGGGGCTCTTTACTGATTCTTCACTATCCAAAAAGACTTGGAGGGGAGACCTGAGCC

[1721] CACTTCTGGAAGGAAATGATAACAATTTATTTAGATAATCTTTGTGCAACAAGTCAATTC(SEQ IDNO.847)

[1722] EGFRvIIIDprobe0848:ACTGAAGAGATCTGCTCTCTAGGAGCCTCTGTGACCCCACCATAACTGGGAAGGCTCTAC

[1723] CTCTCCAGTCTTCGGGCCACATTTCTCTCTGGCCTGCTGTCTTCCCAGCACTCTCAGCCT(SEQ IDNO.848)

[1724] EGFRvIIIDprobe0849:TGCTCATGGAGCACTCTAGTCCTCCGTCGACCTTGGCCTTTGGTAACGTGATTTTTCACCT

[1725] GGCAGCTCCCATCTGGTCTCACTCCCTCTTTTTGTCCAGTCTGCATGACACAGCCTCAC(SEQ IDNO.849)

[1726] EGFRvIIIDprobe0850:ATCGTTAGTGTTCCCTCACTCCCCTCTTACTGCCCAACCTGCAAAGTCCATGCCTGGGCC

[1727] AGTGCAGCATGTGTCCTCAATGGGCTGCTGGTGGCAGTGGGGGGAACCGCACAGCCACGC(SEQ IDNO.850)

[1728] EGFRvIIIDprobe0851:TGTGTGCTGCTGAAGAAATGCACAGCCTCCTACCCTCGCCCTCAAGAGGCAGCCATGGC

[1729] TGCGCATTTCTGCCCTTCTGAGCTCCGCTCACTTTTGGCAGCAGCCGTTCCAACCTGCATG(SEQ IDNO.851)

[1730] EGFRvIIIDprobe0852:GGATCTTCACTCTCTCACAGATGTGCTGACTCCTCCTGCTGCCtcccctctctgtgccttctcactctctgttc

[1731] cctttgccctttctccccttttctcctctgcctacctccaagccat(SEQ ID NO.852)

[1732] EGFRvIIIDprobe0853:ccatcacaggacagctcaagcatcagatcctctgggacactttccttagttgttcagtctgatgaggtgtccctcatcctctcttagcTGAAAAT

[1733] CAGCAGCTGCCTCAACTTCTTTTCC(SEQ ID NO.853)

[1734] EGFRvIIIDprobe0854:AGCATGTCTCATGAGTATTGCCACAACAGCATCTGTCACAATGTGGGGTAGTGGCTGACT

[1735] TGCTTTTCTGCCATTCAACTGAGTTCCCTCAGTGCTGGGGCCAGCGTGCAGTGTCTTGTA(SEQ IDNO.854)

[1736] EGFRvIIIDprobe0855:TTCAGTATATAGCTGATTAATTGATGAATTGATTAATTAATGGTTCACACTAGCACAGTGCA

[1737] ACCTTCAATGCAAAGATCTCATCAAAATAATTCACATGGTGGGATATTTTAGAAGGAT(SEQ IDNO.855)

[1738] EGFRvIIIDprobe0856:GACCAGGCTAGTTTGTAGTAAGAAAAAATCAACAAGACTAGGTCAGGAATTCTTTTTTTG

[1739] TCTACAGGCTTGCTATAGAAGATATTGAAAATCATCTACCTAATTACCTTTATTTTATCA(SEQ IDNO.856)

[1740] EGFRvIIIDprobe0857:GGTTGTGTATTAAATATCACGTCTGGGGGAAGAAAATGTGATATGTGATTACAGACCTTTC

[1741] CTGGTACAACATAGTACGTTTCAGATTAACTCAAGGTATTGTGGTGATATTGCGGTCAA(SEQ IDNO.857)

[1742] EGFRvIIIDprobe0858:AGCCAGGTGATTAAAGAGTCATTCTTTGAAACAAATATCTGTGCAATCAATTAAGAAATT

[1743] AATTTGCAAATTTTATTTGCTTAGAGTAATTGATATATCATTCCTTTTACAAACAAATAT(SEQ IDNO.858)

[1744] EGFRvIIIDprobe0859:AAAGAAAACTTAACTAAAAATACTGCATATCTCTTTCAGATTATATATCCCAGAAAGGATAT

[1745] ATTTTTCTCCTTTCTGGTCTTCCTTTTTGGTGTAGCATCTGTAGGAAATGCATTTCTT(SEQ IDNO.859)

[1746] EGFRvIIIDprobe0860:CATAGCTAAGTGTACCTCCTTGTGAAATATCTTCAGAGTCTACTGGTGCACATAAGCAATT

[1747] GCTGGCAGCAGCTTGAGGGTCTCCATCTCACATTTATCATATGCCTTATTGCATGAGGC(SEQ IDNO.860)

[1748] EGFRvIIIDprobe0861:TTTGCAAGAGGAGGTCTAGAGCTACAATATCTCATGGATATGAATGTCAATTCAAATCCCA

[1749] GTGGCAGTTTATGAGGGGGAAAGCCTAGAAGAGAAGAAACCTAGAGGAATCAAGCAGGA(SEQ IDNO.861)

[1750] EGFRvIIIDprobe0862:GGGGAGAGTAATAAAAGACTAGAGCAGCAGGTTTTTCTTAACTCAAACTAGAATTAAATC

[1751] TCtgtgtgtgtgtgcatgtgaatgtgcccgtatgtgcatgcatgcacgtgtgtaaatgga(SEQ IDNO.862)

[1752] EGFRvIIIDprobe0863:tgtgtgtgtgtgtgcatgtgtgtgcaagtaagtgtgtatacgtgtgtgggcatgtattgtgtacatgtatgtgtgttttatgcatctgtttgcaagtatgtgt

[1753] gtatgcacataaaagtg(SEQ ID NO.863)

[1754] EGFRvIIIDprobe0864:tgaatgtacatgtgtgcttggtgtatgtgtgtgtattaatgtatgcgtgtAGTTCTAGAGTCTAGTTAGAGAAAGTGCATA

[1755] AAGAAATAGGGAAATTAACAAGAAAGCTATAGCTTAAAT(SEQ ID NO.864)

[1756] EGFRvIIIDprobe0865:TATAGGAAAAACTTTTCTCCCTATCAGTCATGGTTTTAAAATGTTCAGACTTGATATGTTTC

[1757] CCAGTGCTATTGTCAGAAAATGTCCCTATGACATTCCATACTACTTCAATCAAATCTA(SEQ IDNO.865)

[1758] EGFRvIIIDprobe0866:AAACCTTTGTTCCAACATGTTTTATTGATATGAGTATATTTCAAATTTCTACCAGGTTTTTG

[1759] GAGAGGTATTTTGGCCATAAAATTGACTAAATTATTCAAAATAAAAAATGAATAAGCC(SEQ IDNO.866)

[1760] EGFRvIIIDprobe0867:TGGGCCAAGGCTTGGAGACTTGCTTAACTCAGTTCTTAAATTTTCAGATTTTCAAAATTAC

[1761] AAATTTAAGCTCTAAAATCATGGTGCTGTGTATGATATTCTTTGATTGCAACTTATGGT(SEQ IDNO.867)

[1762] EGFRvIIIDprobe0868:TGAAAAACTATAGAGGGCTTTATGCTAAGAGTTGTGGATCTTAGGATTTTCATGAAATCTG

[1763] CATTATCATCATCTGCAAGTTTAGATGGGGCATAACTGATCCAAAGGATGGATCCCTCG(SEQ IDNO.868)

[1764] EGFRvIIIDprobe0869:GGGGCAATTCAACTGGCTGATTCCAGCCAAGATGACAACAGTCAGGATCCGTTCCCTTCT

[1765] GATCATCCATTGGGTGCCCTGATTTCCTCTACAGCCCTAGCTGAAAGACCAGACACTATC(SEQ IDNO.869)

[1766] EGFRvIIIDprobe0870:TCAGGCTGGCTGCCCCACATGCCTTGCTCCACACCAAATTCACAGTCTATAAACCTGAGC

[1767] CTCCAGTGCTCCTACTACCATACTCACTCGAACATTCCCGATTCTGACCTGGAGATGTCA(SEQ IDNO.870)

[1768] EGFRvIIIDprobe0871:ACAGCTACTTGATGCCACTCTCTTCTATCTTTCTGTAGCTAAGCCATCCCCAAGTTTGTCG

[1769] ATTCACCCTCTTTAACCCCTGTCGGGGTGTCCATTGTGCCCCTTCACCCTGCCATCTCC(SEQ IDNO.871)

[1770] EGFRvIIIDprobe0872:CTGGTGcactgttttgcaaagttcagcatacatgagcgtcacctgggaaccttaataaagtgcagatgttgattcagcaaatctgggatgccct

[1771] cgggctgcatttccagcaggctcctg(SEQ ID NO.872)

[1772] EGFRvIIIDprobe0873:gggatgtccccgctgctgtgctgcagatgacactctcagtggTGGGACTCCAGGCTCTGCTGTCGCCTCCTAGGG

[1773] GTTTCTCCACACTCCCTGGAGGCCTAATGGGCCCTTCTCCACATG(SEQ ID NO.873)

[1774] EGFRvIIIDprobe0874:GCAGTAAGATCTGTTTTTGTGTTTGTGTTTCAAGTTGGGAGAAGGAGATTATTTAATACTA

[1775] AAATGTGCAACATGGGATTGAGAAAACTAATTATTAGTCATAAGTTGAGTATGCAACAT(SEQ IDNO.874)

[1776] EGFRvIIIDprobe0875:TGAAACCACATGCTTTAAAAAATTATAAGAAAAAATCATAGTATTTGAAAGTTACAAGCTA

[1777] TTATGGCTAACTCCATTTATCTCAGTTAGAGAAGAAGAGTCACCTGTCACCAGGGCACT(SEQ IDNO.875)

[1778] EGFRvIIIDprobe0876:GCCAGAAGCCAGGCTCATTTCCAACAGCACTGGGTGCTCCAGCTTTGGGGTGCCAGCTC

[1779] CTCCCATAAAGCAAACACATACCTAGGGATGATATTTCTTTGCAAGGGCTCTGCCCTACAG(SEQ IDNO.876)

[1780] EGFRvIIIDprobe0877:CTTGTACATCTCAAGAAGTTATGTaattaaactgtctgttttgagaaaattgtagattcacacatactagctgtaagaaatgatgc

[1781] ggataaatccagcgtaccagctttccccacggag(SEQ ID NO.877)

[1782] EGFRvIIIDprobe0878:acgtcttgcagcgtcacagccaggatgaggcattgacccaggcgaagtccagagcacctgtgcgctacagggccccttgcactgtgctgtcaca

[1783] gacacgcccacttccAGATGCCATCT(SEQ ID NO.878)

[1784] EGFRvIIIDprobe0879:AGGACCCCCTCCAAAAAGcagaggcattcttaaaaacacacatctgcacatgttcctcttcatttgaatctgtcagtggcttctcagt

[1785] gcctttcaaatgaaatctaaagtccttacaag(SEQ ID NO.879)

[1786] EGFRvIIIDprobe0880:ccttgcagcaggaacctctccatcccacttcccctcacactctcagcttcatctctgctaggctctgttcagccaggcagcctttcacagtccctctcc

[1787] tcctgccctgccaggaaggtc(SEQ ID NO.880)

[1788] EGFRvIIIDprobe0881:ccctgcccccaactcttccccacatgtggcggggccccgcttgtccttagaagcccagctgaactgcttcctgaaggaacccctccagaacctct

[1789] cagaccaggtcaggtttctgcactc(SEQ ID NO.881)

[1790] EGFRvIIIDprobe0882:ttagatcatccccatggcataatcacagttgtgatgttgtgatgattcagtgaatgtctgtctccccactggatggtaagcttcctgagggcaggaac

[1791] agcattggttccagtcaatgct(SEQ ID NO.882)

[1792] EGFRvIIIDprobe0883:atgtcccAGGACTGTTCGTTTTTGCACATACTAATCCTAAAAGGACGATGACAACAGCAACcac

[1793] ttacatgacctagatgctcttctgggtgttgtgcaaatattaacaatttaatcctt(SEQ IDNO.883)

[1794] EGFRvIIIDprobe0884:gcaacaatccacgagggaggcattcttctactcccacttaacagacaaggacagtgaagctagtaaagagaagtcatttgcccaaggggacccc

[1795] actactgttggcagagctgggtgcaa(SEQ ID NO.884)

[1796] EGFRvIIIDprobe0885:acgcaggcttgtgaagccaggacccatgcattcaaagaccatgccaGGTGCCCCCACTGCACACCTCATCCCCACA

[1797] TACCAGTGAGGGGGAGAGAAATGCTCCTGCACTGCCTCTGATTA(SEQ ID NO.885)

[1798] EGFRvIIIDprobe0886:ACTGCTTTCCTAGAAGTCACACATATAAAAGGGATTTAATTCTAGTGGGATTGAATCTCAA

[1799] TAGTTTCCTTATTAGGTTGATTTCTGTTAATAGTTTAAGTACTGGATATACATGAATTA(SEQ IDNO.886)

[1800] EGFRvIIIDprobe0887:GAAAATCTAGATTATTAGCAAATGCAAACTATAAAGTATTTTATAAATGTTATCTTGTTTGT

[1801] CAGGGGATGAGTGAGATATTCATTATACAAAAAGTAGTGTGGATTTTGAGGTAGAAGG(SEQ IDNO.887)

[1802] EGFRvIIIDprobe0888:TTTACTAAGGATCATACCGTAGTATGAAATAGCCACAAACATTCAGTGAAACCAAACACC

[1803] CCCGCTTAACCTCAAACTAACACTAAATAATAAGGAATAGACTTGGGGGCAGTGCAAGTG(SEQ IDNO.888)

[1804] EGFRvIIIDprobe0889:TATTTCTAATGGTGAAAACCATTCCCCAGTGAAAACTAATGTACCATCTAGTTAATAAGAG

[1805] CTCCTCTGACCCACGCACATCAATACTTACATCCCAATGGTGATGTGACATTTTGGGTT(SEQ IDNO.889)

[1806] EGFRvIIIDprobe0890:TTGTATTTCTTTTGCAAATTGAGCTAGCATTTTTGATGAGTGGCAGGGCTCTGCTACCCAA

[1807] CCTTTGGACAGTTTCCAAGCATAAAATCACAATTCCAGATAATTCTGTCACAAAGATCT(SEQ IDNO.890)

[1808] EGFRvIIIDprobe0891:GGGTCTCATTAGGAAGGAGAGGAAGCTGGGAGATGATCCAGTCCAACCTCCCCCAAACC

[1809] AAACATCACGGCCTTCTCAGTTGTTTCACCAACCATCTAAATGTTTTAGTAATTCTAAAAA(SEQ IDNO.891)

[1810] EGFRvIIIDprobe0892:TTGATGCGCTTTTTCCACGAAAGGAAGTGTTACCACATTTTCCAAGTGGGAGGCATCTATA

[1811] TCCTTACTCCTTCATCCTCTCCTTCCCACCCCCTCACCCCCCACCACCCACACAACATC(SEQ IDNO.892)

[1812] EGFRvIIIDprobe0893:TGCAATTCTTAAACTAAAGCACAAATTGTTACAAAAGTTAATTGCACTTTCAAAGGAATG

[1813] CTTGTATAGAAACTTTCTCGGCTTCAAGGAAAAATAATACGCTTTGAATGGCTGTTCAAC(SEQ IDNO.893)

[1814] EGFRvIIIDprobe0894:AGCATAGAAATTAGCTGAGTAGAAGGCACTCATATAGCCATTAGGACCAATCCTTTCTGCC

[1815] GCCAACACCCCCCTTATAAAGACTTGACAGTGGGCCAGAATAAACAACTTCAGGATGAA(SEQ IDNO.894)

[1816] EGFRvIIIDprobe0895:TTCAGTTGAGACACAAAGTACACACTTCCAGTTTTTCCCTTCTCTGGTTACTGGCCTCAAT

[1817] AACCAGGCAGTCAACTTAAAAAGAAAAACAAAAGCTTGCTTCAGATTACAGATTGCAGA(SEQ IDNO.895)

[1818] EGFRvIIIDprobe0896:CTTCTTATAATATGTCCATTTCACCAGGCCCCGCTCTCAGCCCCGGGAAAGGCCACTGGA

[1819] AACCACCTCACATGGTAGGGCCTTGCGGGAGCCAGTAATAACCTTATCTCCGTCAACATG(SEQ IDNO.896)

[1820] EGFRvIIIDprobe0897:TTCTGTCAGATTGAATGGGGCAGCCAGAGAAGCCAGAGTTGGCACAGGAACCAAAACA

[1821] AAGGCTTCCCATCCTCCTGGAGTGAGCGGTTGAGCCTGGATTGGTGCTTAGACCTATAATGG(SEQ IDNO.897)

[1822] EGFRvIIIDprobe0898:GTGCAAGCAGCGTTCATTCATAGTGGCTTTCTAGACCCAGGGACTTGGCCCCAGCCCTGC

[1823] TGCTCCACTCCTCTTCTTGCTTCATTACCACGAGTCTCCTAGACCACCGAACGATGCCTG(SEQ IDNO.898)

[1824] EGFRvIIIDprobe0899:CATTTGAAAGACACTTCTGCTGATCAAAGCAGCTGATGTGTCCCTTTGCGGTTCATTTCTA

[1825] ATTGTCCCCAAGGAGGAGAAATTCAAATAGTTTATTACTGAGAGTTAAAGAAATCCACT(SEQ IDNO.899)

[1826] EGFRvIIIDprobe0900:GAAATATTCTTTGGTCTAAAATTACTGTCATGGCGGAGCAGCTTCACCTTAGTCATTGCCC

[1827] TTAAATATGAAAGCTATTTAAGAAAGTTTGCCCTTAAATATGAAAGCTATTTTAAAAAG(SEQ IDNO.900)

[1828] EGFRvIIIDprobe0901:TTTAATGAAAGAAGAGAATCACAAAACATTTTCAAAAAGCAAAAGAAAACCTAAGAGA

[1829] AAAGTTGAAAGTAGGAATTTTTTAAAGAATATACGACGTGTGTTCTGTGACTCACCCCTGCA(SEQ IDNO.901)

[1830] EGFRvIIIDprobe0902:AGTTATTTGTGTGTATTCCCTTGCATAGTAATTAATAATGAAGCAAAGCATGGCAATGATAT

[1831] CTTTTCTTGTCTAGTATTCTAGAAGACTCCATGTTTTTGGAAAATATCACTCTAGTTA(SEQ IDNO.902)

[1832] EGFRvIIIDprobe0903:GATCTCAAATATATTCAATCAGAAAATGGGTTTTCTACAAGATTCTATATCTGTAGTCAATA

[1833] GCAAATATAATTCTATTAAGCTAGTAGGATGTGATAGGAAACTAAAACCTAGGGGAGA(SEQ IDNO.903)

[1834] EGFRvIIIDprobe0904:CCAAAGCAAGGAAAAATACTTCCTCATCCAAACTTGAGAGCAATTTACCGTCAGGCCTAC

[1835] TATTAATAGATGGAATACAGATTCCATTTTCATTACTCAACTGCCATATTCATTATTACA(SEQ IDNO.904)

[1836] EGFRvIIIDprobe0905:CTGTACAGAAAAGGGAATCACATCTGTTGAAAACTTATATATGATGTTCATGCATGCATTC

[1837] CAGTAATTCAACAATTTTTATTTATCTTTTTATTGCTTGCTAATTTTTCAAAATAATAA(SEQ IDNO.905)

[1838] EGFRvIIIDprobe0906:GCTAAAGAAAACAAAATGTTTGTGCTGTTCTCAGATGACATGTTATCTCTTTAAAGGACA

[1839] AAATGTGCTGTGAAATAATAGAATGCTTTCAGCACTCAAGTGTGAGTGAGTGCTCATACA(SEQ IDNO.906)

[1840] EGFRvIIIDprobe0907:TGAGAGAAAGCCGTGGGGACTACAGAAGCCAAGAAGCAGATCTAGCTGGGGAGGCCTT

[1841] TGCAGAGGATGTAGTTGTGTGGAGAGGCCACACACGTGGAATTCCCAGGAGGGCTGTGGAGG(SEQ IDNO.907)

[1842] EGFRvIIIDprobe0908:CGGGGAATCTGCAGGAAAGCACTGGGGTGAGAAACGTgatgagaaacaattattgtcttaaaatatctgcagg

[1843] gctgtaaggtagagaagcaatacgttgcatctgtgttaagtcaaaca(SEQ ID NO.908)

[1844] EGFRvIIIDprobe0909:aaattatcaagggactggtttcagcttaacataaggaacaattatgtgatagggttgtcaataacaagagtagactgcttcttcacacactcctagtca

[1845] ctcagaatggtccaggaggag(SEQ ID NO.909)

[1846] EGFRvIIIDprobe0910:tggacaaccatttggtagagTATGGGAAGGCAGGGGCCCTGGGTGGGAGTGGTGAGGGTAGGGAGT

[1847] GAGTATCCCAATCTAGAAGTAAATTGTGCCCAGCACGGAGCTGCAACACTGCCC(SEQ ID NO.910)

[1848] EGFRvIIIDprobe0911:TGCACACAAACACACACAAATAACAATCCCCAGCCCCTGCATTTCCCTCTCCGGTTTCAG

[1849] GACCTTGTATCTTACTTCAATTCCTTTATTTAGCTGATGATGAAATAGGAAGAGCTTAGC(SEQ IDNO.911)

[1850] EGFRvIIIDprobe0912:ACTAAGAAAATCCTTTTGGAGTTTGGCCTTGGGGGAAAATGAATCACTCCAACCAGGTCT

[1851] GTCTTCTAGAAAGTATAGGATGAAAGGGCTCCTCATCACATACTTCCTGACCTCCTGCTA(SEQ IDNO.912)

[1852] EGFRvIIIDprobe0913:GGCCTTTCCCTAAAAcaggggctggcaaagcacaacctgtgggtcacgcctagcctgccacctgtttttgcaaataaagttttattgg

[1853] agcatgactatatgtatttgcttacagtctgt(SEQ ID NO.913)

[1854] EGFRvIIIDprobe0914:ggctgcgttcacactatcccagcagagttgaataattgggacagggaccatatgatgggtgaagctgaaaacatttactctctggctgtattcagag

[1855] gaggtttactgagccctTCTCTG(SEQ ID NO.914)

[1856] EGFRvIIIDprobe0915:AGACATGGCAAGCGCTGCTTCAGGCTCATGCTTCACTAGATTCAGGCCTGGGGCAGTAA

[1857] AGAGCCAGCTCAGGATAGCACTCCCGACTCACTCATTTTTTCAGGCAGGGGAGCCATCTAA(SEQ IDNO.915)

[1858] EGFRvIIIDprobe0916:TGTCAAGTGCCTACGTGCAGGAACTGGTCTGTTAATTAGCAGCTCTCCTCATGGAAGGGA

[1859] TAATATATTCTAGAAACAGGAGTGCGGCCCTATTGCAAGAATGTCCTGAGCCAAAATTAA(SEQ IDNO.916)

[1860] EGFRvIIIDprobe0917:GATTCTTCTATGGCAGAAACTTGGCTGGGGCTTCTCCTGAGTTAACTTGGTAGTTGTTAGT

[1861] GATTTTTGAGTCAGTTTTTCCTTGTCAACGACCCCAGGAATGAGTTTGGGATTACAGGG(SEQ IDNO.917)

[1862] EGFRvIIIDprobe0918:TAGCCAGGGAAAGGGAAAGCTTCACGCCCGCCCCCGGGACAAGGTCTGTCTTCACACTG

[1863] CTACATCCCTTCACCCACTTTAAAATGAAACTTAAAAGGAGGATTTCAGTTGAGTAGGAAG(SEQ IDNO.918)

[1864] EGFRvIIIDprobe0919:TGAGAAGAGGGCTCATTTTAAAACAAGCGTTAAATGAAAACCCACACACACTCAGAGCA

[1865] CACAAATCCAACCACGCTTACAAAACCATCACAGAGGGTCAGGCGAGGCCCTTTTCTAAAT(SEQ IDNO.919)

[1866] EGFRvIIIDprobe0920:GAAAAAGAACAGGGGTGGAGACTGTTCTGAGAGCATGCTGGGTTCCCTGAAGGGAATT

[1867] CTCAGCTGTATGTGCCCCGCACAGGATCCCTGCTAGACACAAGGCCAGCTGCCTTCCTTTCA(SEQ IDNO.920)

[1868] EGFRvIIIDprobe0921:AGCCGCAGACGCATCCCTGTGTCCAGGCGGGCTGGTCAGCTGCGGTCAGCACCAGCTTC

[1869] CCCGCTCCATGGTGAGGTCATCACAACATGTGAGCAGGAGGGCAGGCCGGCAACCTCTGAG(SEQ IDNO.921)

[1870] EGFRvIIIDprobe0922:TGCTTAGAGAAAGGGACGGGATTCCTCCTGTGCAACCCCTCTAGTCTCACTCAGACTCA

[1871] AGTCTGACTAAGGGGCCAGGTGCTTTGACCAGGGACTCTCCCCTCTCACTTCCCTCCCAGG(SEQ IDNO.922)

[1872] EGFRvIIIDprobe0923:AGTCAcaggtacatgagtccttgttttacaaatgaagaaaacagacccaacatgattaagatgttgccttcataggggtggcaccaggattcca

[1873] aaccatggactccactgagcccagtg(SEQ ID NO.923)

[1874] EGFRvIIIDprobe0924:cccactgACATGTGCCAGTAACAGTGCAGCTGCCTGTGGTTCTGTCGACTAAACTGCCGGCA

[1875] GAGGCTGGCTTTCCACCTTCTTTTTTTTTTTTTCACTCTTCAAACACTTTATGACATG(SEQ IDNO.924)

[1876] EGFRvIIIDprobe0925:AACATAAACTACTGGCTGCATCGTTCTGCTGACAACATGACATGTTTCTATAACTTGAAAA

[1877] AAGCAAGCAGTGGACTGCTCATTGGTAAAATTGAGTCAGTAATCTTTTAGGAAGGTTAT(SEQ IDNO.925)

[1878] EGFRvIIIDprobe0926:TTTTCTTCCTTTTACTGCTTCTCATCTGTTCCCCGCAGTAAAGAGGACAAGATGACGACG

[1879] ACTCAGGGAACACCTCCAGCCTGAAGCAGCACCATGCGAGCTTAGACCTTAGGGTCGGCT(SEQ IDNO.926)

[1880] EGFRvIIIDprobe0927:TAGAAACCACAGGCGGGGCGGCTTGGGCCCCTCGGACACTCCCTCTCGAAGCTGCTTCT

[1881] CCCCAAGCTACCCCAAAGGCACTGAGCGCCCTCTGCCCCCCAGCAATTCAATTCACTGGCT(SEQ IDNO.927)

[1882] EGFRvIIIDprobe0928:GTCCTGCTCCTGTCAGTACTGAGAGTTGCATGTTTGACCCTCGGGGGAAAAGTCCAGAG

[1883] GCCCTGGGGTGTCCAGCATGCTCTGAGGTCCCTGCTGCTGACCCCTTGCGCTGTCAGCATT(SEQ IDNO.928)

[1884] EGFRvIIIDprobe0929:CAGAGACATTCACACAGCACAGCCTCCCAGGCTAACAGCTGTCATGGAACAGTGGAGCA

[1885] GCTAGACGTGGCCATTCTGTGGCCCAGTGCTGCAGAGGTCAAAGGGACAAGCGCAGGGAGC(SEQ IDNO.929)

[1886] EGFRvIIIDprobe0930:ATCTTTGCTTTCAGAAAAAAAAAAAAAAAAAAGAAGCACACTGGTGCACTGACCTGCTC

[1887] CTGGTGTCTTTGTGATTGCTCTTTTCTTTCGATTTTTGGTTGTCTTTTTTTTTTTGAAAGA(SEQ IDNO.930)

[1888] EGFRvIIIDprobe0931:GGGGCTTTTATGCTTTTTTCCTAATGTTCATGGGTAAACCAATGTAAATGTGTGTATGTTTA

[1889] TAGAGATGGCTTTAAATCGCAATTCTGCAGTAGAGATTGATTTTTTAAAAAACATGGG(SEQ IDNO.931)

[1890] EGFRvIIIDprobe0932:TAAAAATTGAAGAAAAATTTTAAAAGAACATTTAAACCATCTTGGGCTAGGGGTGGATAT

[1891] GCACCACCCCACGGAAGCCAAACAAAATCTCTCTGCAGATAAACATTTGCAAAAAGAATT(SEQ IDNO.932)

[1892] EGFRvIIIDprobe0933:TCCAATCCCAATTTTTGAGTCAGAGATCTTTTATTTCCTTGCAAATTACATATCTGTTTCAG

[1893] GATTTTTGACTATAAGAAGAATGAATGAAGATGTGTTTCTTACAGATAACTATGAACA(SEQ IDNO.933)

[1894] EGFRvIIIDprobe0934:AACCAGGAAGGATAATAACTTGTATCCCCCAATTCGAATCCAGAGGATGGGAAGGCATAA

[1895] AAAAAAGAAATGGAAGAAACTTTATTTTTAGTGGTAAATGGTGGGACTATGTATTTTACG(SEQ IDNO.934)

[1896] EGFRvIIIDprobe0935:TATGGTGAAGTCACCAAGCCCAACACTTGGCACTTGTAGGCAAGGTAGTCTTCTAATCTG

[1897] AATGTGAAGTATTATGTTTTCATTTGCTTGGTAATGAGGAATATTGGTGCTTTCGTCCCA(SEQ IDNO.935)

[1898] EGFRvIIIDprobe0936:GTTCTCGAGCTGACTGACTTCTCTTTCTGACGTGTGTTCCTTTAGCACACCTCTACACTGC

[1899] ATGGCTCTGAGATGTCCTGTGACTGTTTCATGTGTAAAGTTGCCTCCCCAAAGGACTCA(SEQ IDNO.936)

[1900] EGFRvIIIDprobe0937:CATATTCCTTCAGGGCAGTGAGTACTTCTGATTCATCCTTAGCAGCTACCTTCGCGCTACT

[1901] TTACTAGATATGTTGTAGTTGAATTAATGAACAAAAGAACAAGCAACTTTGGTGCCTGG(SEQ IDNO.937)

[1902] EGFRvIIIDprobe0938:TGTGCATCTCAGAGCAGGGTGGAGTGAGCCTGGCCAAAGGGTCATCATGCAACCTCTGT

[1903] GGCTGACTCCATCTGGCCACGGAGCTTCTCAGCCATGCTTGGTATTCACATGACTTCTAGG(SEQ IDNO.938)

[1904] EGFRvIIIDprobe0939:GCGACAGCTCAACCAGCAAATAAACAGCTTCATATGGGAAATATTACTCAGCCTTTGTCA

[1905] TCAAGGAGTGAGTCACGGGCCTGAACTGAATAGAAGATAGAGGAGAAAAGGTGTGTGGAC(SEQ IDNO.939)

[1906] EGFRvIIIDprobe0940:TGGGTGAGACAGCGCCCAGCGAGGTGAACTCCCGGCAGCCCTGCCTGTCTTTACCTGCA

[1907] CATCACCTTGCTAGGGTGCCTTCGGTTGTGAGGGCCTGTCTAGGAAGAGAAGAGTTGCACC(SEQ IDNO.940)

[1908] EGFRvIIIDprobe0941:CTGGCAGGCAGCACTGAGCTGTCTCATGCAAAGCTGAGGAAGAAAGAGTGAGCTGCCC

[1909] AGTGAGCCTGCTGGGGTGGTGGAGGCTGGGCTGGGCTGTGCAGTCTGCAGCCCCCAGCAGCC(SEQ IDNO.941)

[1910] EGFRvIIIDprobe0942:CTTGGCACCTTTCTACTGCCTGGTGCTCACCAGCTCTCCAGTAACAAAGAGGGACGTGA

[1911] AGTCAGAGGGGAAGGGAGGTAGCACAGGGCAGTCTTGACTTTGAACAAAGAGCTGGCTTCC(SEQ IDNO.942)

[1912] EGFRvIIIDprobe0943:TGAAGTCAGCTGGCCGGGTTTTGAAGCCGATTTTCCAGCAGTGATCTTTGATGCCAACCC

[1913] CATTTAGGAATTCTGTATCTCCCCCTACCTTCTACCAGATGTCTCTGAGCTCACCTTTGG(SEQ IDNO.943)

[1914] EGFRvIIIDprobe0944:TGATAATCATGCAATCTCCGTCATCCCCACGTCCACACTGCCCCATTCTGTCCCACCCCGG

[1915] GTTCTGTGGTGCTGTCGGCTCCCCAGCGAGCCAGGAAGGGAGAGGCCAGCTCTGCTGGG(SEQ IDNO.944)

[1916] EGFRvIIIDprobe0945:GCTCCTGCCGCCCTGGCTCTGCACTGCCCTTCTCTGGCAGGTCTGAGGCGCCACTGGAG

[1917] GAGCCACACGGCCCTGAAGCAGCAAGGCAGATGCCCTGGACACAGTGGAGGCACAGAGTGC(SEQ IDNO.945)

[1918] EGFRvIIIDprobe0946:AAGCACCGGCCTGGCCCACAGACTTTTGGAGGGGAAGTGGTATTATTCAGTTCAAAAGT

[1919] ATGCCTGTGTGTAAAGAGAGAGCCCCTGAACATGAGTAAGCAAAAGTCTCAGCGCAGAGAT(SEQ IDNO.946)

[1920] EGFRvIIIDprobe0947:TAGACAAGTAGAATGCTGGCCCGAGAGGAGGCGTTTACTCACCCTCTGTCTAGGAAGGA

[1921] AAGCCAGGCCCAGCACGCTCACTGCTATCTATCCTCTCACACAGAGGGATTTTGAATCGAA(SEQ IDNO.947)

[1922] EGFRvIIIDprobe0948:GCCAGCATCCTGTCCTTTCTCCAATGTCCCCTGCTCAGGAGTCAGGACTCAGCAAGGCCC

[1923] ACCCCAGCCACACACAGATACAGTTCCAGGACTCAGAACTCAGCGAGGCCCACCCCAGCC(SEQ IDNO.948)

[1924] EGFRvIIIDprobe0949:ACATGCAGGTCCAGTTCCAGGATTCAGGACACAGTGAGGCCCACCCGAGCCACATCCAG

[1925] GTCCAGTTCCAGGACTCAGGATTCAGTGAGGCCCACCCCAGCCACACACAGGTCCAGTTCC(SEQ IDNO.949)

[1926] EGFRvIIIDprobe0950:AGGACTCAGGACTCAGCGAGGCCCACCCCAGCCACATGCAGGTCCAGTTCCAGGATTCA

[1927] GGACACAGTGAGGCCCACCCCAGCCATATCCAGGTTCAGTTCCAGGTAAATCATCTGCCTT(SEQ IDNO.950)

[1928] EGFRvIIIDprobe0951:CCTCCGTCCAAAAGCCTTGTTTCCTGTGTGTCCTTGTGTTTAAAATGGAAACGTTATGAG

[1929] AAACTGCCTGCCAGGGCAAAGGGTGCTGCCCGGCACACAGTAGGGACTCAAAATGAAACT(SEQ IDNO.951)

[1930] EGFRvIIIDprobe0952:ATTGTATTGAATACATAACAGATCAACGGGTATTGCTTTCTGAAATCTTTTTTAGCCCAATT

[1931] TTGTTTCTTATAGTCCAATAACAGGTCAAATTCATTTCTGATTTACTAGCCATTCAGT(SEQ IDNO.952)

[1932] EGFRvIIIDprobe0953:TGCCCATAAAAAATGGAAAGTGATTTAAGATTATTAGTTTAAAAACCAATGAAGGTAAAA

[1933] CAGTTATCATTGAAGGCACATAGGCAGAAATAGATTGCAATAGTTGCTGCCATGTGAAGC(SEQ IDNO.953)

[1934] EGFRvIIIDprobe0954:CTCAGTGTCATGCTCCATATTTAGAGAGAtctatgatttctgaggccctttcatgtccatgatctcagtactgctcacaactg

[1935] ccctgtgaaattcgccgagctggccccatgtcaatca(SEQ ID NO.954)

[1936] EGFRvIIIDprobe0955:gagtacactgagcactgagacccagcatgttgagataactggctagagatcaTCCCATAATGGTACCATCACAATCTTCA

[1937] CACTGTAGAAGTTTGATGATGTCACTGGAAGCATATTCCA(SEQ ID NO.955)

[1938] EGFRvIIIDprobe0956:CAGTCCCTTGTGAACTGGCCTTCCTGTGATCAGAAGCATCAGTGAACTCCCAAGAGGGT

[1939] GGGAACTCCCAAGAGGTATTCTCACTCTACTTAGTGTATATTTTACAAATCACAAGCTTGG(SEQ IDNO.956)

[1940] EGFRvIIIDprobe0957:CTTTGGATTCTTTTAATGGCTAGAAGGAGAATCATGGGGTTGGAAGTCCACCAGTTTGGG

[1941] TATTCTGTTCCCTAACTCAAAATAAAGAGATGTTATTTTCAAGTCTTCTGCTTGTTAACT(SEQ IDNO.957)

[1942] EGFRvIIIDprobe0958:TAATTAGAGATACATGAGTTTGCAGCTGTGCTGGGCATGCCGCAGCTTGGCATGTTTAGTC

[1943] CAGAAGGCATATTATAATGTACATGGAAGATTGTCAGAAATTCAAAAGGACTTTTTGAG(SEQ IDNO.958)

[1944] EGFRvIIIDprobe0959:TATCACATGTGTATTTTCAAGTTCCAATATAGATTCACATTCAGTTTGACAGGTATCTTTGG

[1945] ATGCCTATCAGTTAAGAACTATTTATTAGTTGTGGAATAAAATAGGGTAAAATAAGGA(SEQ IDNO.959)

[1946] EGFRvIIIDprobe0960:ACAACTGAGGAAAAAACATAAAATTTGCTTTGTGAATAAAAGTTGTCTTCAAAATTATGA

[1947] CTTTTTCCATCCCACAAAAGTTTTGATTAAACCCACAATGAAAATTTAAATAAGTGTATT(SEQ IDNO.960)

[1948] EGFRvIIIDprobe0961:TACTTTGGTTTAACCACTTATTTCATTATGACTCACAACTATAGGTTTTCTAGTTTCCATTAT

[1949] TACAAACTATTGTGTGGTTTAAATCAATTTCATAGACTAGTCTAGTTCTATAGTCAC(SEQ IDNO.961)

[1950] EGFRvIIIDprobe0962:AATTTATAAAATTTTTTTATGTGGTAAATTGAGTGTCTTCATAGATGTACATGATTATTTCTC

[1951] AATTTTTAAGGAATGTATTTTTTAAGATAGCCTTCTTTAGCCTTCTTTAACACTGAT(SEQ IDNO.962)

[1952] EGFRvIIIDprobe0963:TTTTGTAAATTTTTTACAGATTTTTTTAAATTTTTGGTAATTTTTTAGCATaaagtaatacatggtcacta

[1953] tggaaaacataaaaacacaaaaactatgaagagtaaataagaaaaacac(SEQ ID NO.963)

[1954] EGFRvIIIDprobe0964:ccagaaatttaccattcagaaaaggtcattgttaacaacacggtgtatcttcctcctgtcatgcttccgtgcatTTGAGCACATTTGAGA

[1955] TGTGTATACATGTTCACTTTGAGATTTTAG(SEQ ID NO.964)

[1956] EGFRvIIIDprobe0965:TATAGCAAAAGAAATGACCGGTCCTGATTCAATGAAACCTCTGGCAAACTCGCTATATTTT

[1957] CCTTACATATTTTTAAGTTCATCCTATAAATGAACTATCCATTCATCTTATTTGAGATT(SEQ IDNO.965)

[1958] EGFRvIIIDprobe0966:TTCTTAAATCTTTCAGCAAGAAAGCGGGAAAAAAATCCTCCTCTGGCCTTTAAAGCCTAA

[1959] TTAAATATATGACTAAGCTAGAAATATTTTATAATGACCAACCAGAAAGTGGCAAGGACT(SEQ IDNO.966)

[1960] EGFRvIIIDprobe0967:GTCACTCTTCCCATACAGCCCACCTCCTCCTCTATCTCCCTCAGGCACACGGAAACGAGA

[1961] AAGGCAGAGAAACCCAGGACAAGTCATCCAAGACTTTGGTCACATGGCCATCCATTGCTT(SEQ IDNO.967)

[1962] EGFRvIIIDprobe0968:TCACAACAAAAATATAAATCCAACATGTGTGTGTGCATTTCATACCAGTAGGTCCAATAAG

[1963] CTATCTATATATACACATATGTGTACACACACACACACACATCCTTAcagacactcccc(SEQ IDNO.968)

[1964] EGFRvIIIDprobe0969:agcttactacagtttgacttaagattttttgactttacgatggtgtgaaagcaatgcacattcaatggaaaccatacttctaatgttgaattttttatcttttct

[1965] tgggttagttgatgt(SEQ ID NO.969)

[1966] EGFRvIIIDprobe0970:ctgatatgttactttcttgcgatgccaggcaatggctgggagccagagctcccagtcagccatgcaatcaagaggctaaacagctgatactataca

[1967] gtggactgtgtcaccagcattttg(SEQ ID NO.970)

[1968] EGFRvIIIDprobe0971:gggatattgtgttttgtgtttttgaatcctatcatgtctacaaaatgccattttcgactgctattttcaatttagggtgggtttatcaggacataaccctatgg

[1969] aaagttgaggaccatc(SEQ ID NO.971)

[1970] EGFRvIIIDprobe0972:tgTATATCTGGTAGGGAAAGATGGATAACAAATTCATAGGCAAATAATAATTTCATGATTATT

[1971] ATTAAGTTATTCCTACTTAATAATAAGTAGTGATCACTGCCAGGGAGCAGAGAATGC(SEQ IDNO.972)

[1972] EGFRvIIIDprobe0973:AGGATAATGTGACAGATGTAATGGTGGGTACTTAAGCTAATGTAGTTGCAGAACAGGCTT

[1973] TTCTAGAGGGTAGGCCTTTAAGCGTACCTCGAAGATGCAAAGGAAGCAAAGATGCGAAGA(SEQ IDNO.973)

[1974] EGFRvIIIDprobe0974:TCTGGGCTGGGGATGGAAGCAGAGACAACTTGGAGGCCAAGGGGAGAGACTGACAACA

[1975] GCCCAGCTCATACCTCAGCAGCCTTTAATGCATAGCTAAGAAAACAACAAATTAAAACAATT(SEQ IDNO.974)

[1976] EGFRvIIIDprobe0975:ATAGTTTACTTAGACGATTCTAAGTGTCTAAGTGGATTTGGGCAAATCTGGAGAAACTTGT

[1977] TCTAATACTGTGTCTTAATAAGTAATATAGATTTGCCCAGGCTTGTGGGCAGAGTGGTA(SEQ IDNO.975)

[1978] EGFRvIIIDprobe0976:TACACCCCATAATAGCAGAGGAAGGCCACAGGGCCTACCCTACAAAACCAGAGGCATTT

[1979] AAAAACTTAAAGGAGGCAGATTGCTTTTATTTTCAGTTAAAATAAAGTGAGGAGTTTCTCA(SEQ IDNO.976)

[1980] EGFRvIIIDprobe0977:AGAAAAATAATAACGAGACCACCGGCCCGCCCTAGATGTCCAACAAGAATGCACAGATA

[1981] ACTTCGTATATCCACTTTCCTGAACCTGCCCCTGACAGCCAAGTGGAGcacaacaacagag(SEQ IDNO.977)

[1982] EGFRvIIIDprobe0978:atgaacctcaaaactactgtgctgtgacataaggcttgctcaagaggacagtgtggtgtgagtccatctatgttctaaagcaagcaaagctattctgt

[1983] agtgaaaatggatcaggacagc(SEQ ID NO.978)

[1984] EGFRvIIIDprobe0979:agttgcctctggtgtatgggggcagggatcgactgggaggggcatgagggatgacagttagggtttcgatcatgacaggaattcagattactcca

[1985] gcatgtgcatttgttaaagctcatc(SEQ ID NO.979)

[1986] EGFRvIIIDprobe0980:aaatgctacacttaagattaatcctctcacagtttgtggatgttaccttaaaaacaacaatgatgactgcaaactaatattgaactctggttagtgatata

[1987] ccaatgtgaagtatagtga(SEQ ID NO.980)

[1988] EGFRvIIIDprobe0981:tatctctactttactttaaaatgcatccaaaggcagactagaggaccatatctgacagacagaaaaatagatatgtgataaggtgaatgtagtaaaat

[1989] gctaacataaggatgtttgcgg(SEQ ID NO.981)

[1990] EGFRvIIIDprobe0982:tacaattctttcagcttttctatacatttataaatcataataaaattttaggacaaaaaGTTAGTGCTTTGAAGTCCTAAGTCATA

[1991] GGGCCTGCTGCTCTTGATGCAGTAGAATTTGTCT(SEQ ID NO.982)

[1992] EGFRvIIIDprobe0983:TCAGATTTGCAAAGGGTAAGGCAAACCACTAGCATTTTGTATGGAACTTGATGCAAATAC

[1993] TTTTAATTGTCTGGTTTTCAAATGTATAGACTTAAAGTAATATCAACTCTTTCTTTGAAT(SEQ IDNO.983)

[1994] EGFRvIIIDprobe0984:CAACTACTGAAATACCTAGTCTTAAATAAATATTTTTATGTAATCCTTAAAGTACTATGTATT

[1995] CATTTTTCTTTCTTCTTTCTTTTCTGGTTTGATAAATATTCTATAAAGTAACTGTGT(SEQ IDNO.984)

[1996] EGFRvIIIDprobe0985:TTAATGGCCAACATTTGAGTAAGTCCATATGCAGATCCAAACATCTCAGTTTAGACAATAA

[1997] CTTAAGACAATATAGAGTGGCTGACATCCCCTAACGTGGGTCCAGATGCATGTTATGTT(SEQ IDNO.985)

[1998] EGFRvIIIDprobe0986:ATGTTTCTGTTGCATTCTCAATAGTTAACTTTAATAAAAGAAAGTCAAAAGCTTATAtattttttc

[1999] aatcttcaaaacatttctgggaggttgtcttagttaattttatgttgctatacc(SEQ ID NO.986)

[2000] EGFRvIIIDprobe0987:actgggtaatttataaagaaaataaatgtatttggctcatggttctggtggctgggaagtccaagagcatggcattggcatctgcttggcagctggtg

[2001] agggccttcatgctgtgtcaat(SEQ ID NO.987)

[2002] EGFRvIIIDprobe0988:ctgtgtcaatctatggtggaaggtcaagagagcatgcatgtgaggtggtggggaagagaaaaagcgggtttaactcatccttttatcagggactca

[2003] ctcccgtgatagctaacccattct(SEQ ID NO.988)

[2004] EGFRvIIIDprobe0989:tacatgaatggcattaatccattccttagggcacagctctcatgacctaattataatacctcttaaagtttccacctctcaacactgttgcattggtgatta

[2005] agtttccaataaacgcac(SEQ ID NO.989)

[2006] EGFRvIIIDprobe0990:tttggaaaacacattcaaaccacagcagagatcaacgttattgtcaccattttcatatttgaggaaagcatggcacagagagcttggagaagtacttc

[2007] aaggtcacccaatgaggaagtg(SEQ ID NO.990)

[2008] EGFRvIIIDprobe0991:gctaaacaaaaaccttatcttaaattaattaaaaacctcttgctctttgcaGTTTTGTCTTAAATCTACCTAATTTGTGACT

[2009] GTAATTTTTAAGTAATTTACTCATATAAGTGGTCTCAC(SEQ ID NO.991)

[2010] EGFRvIIIDprobe0992:ATTAAATTTTCTCATTGCTTTATATTTCTAACATGAGATATTTGGTATAAGGATGGAACCAA

[2011] GATCATACCTTGTTTTAATTAGAAAACCTAGACCAAGTCATTGTGATCCTCATCCTAG(SEQ IDNO.992)

[2012] EGFRvIIIDprobe0993:ATTTCAGTTAAATGCTGCTGTCTCCTTTTGGGTATGTGACAGGGGAAAGCCTCAGAAGAA

[2013] ACAACCTTATGTGTTTTCTTTTGATACTTTAGTAATTAACCCAGGATAGTATTCAAGATT(SEQ IDNO.993)

[2014] EGFRvIIIDprobe0994:GACATGCCTTATATTGAATCAAATAGCATATCAACTGCCTTCTTATTCTCAAGTATAGACAT

[2015] GTTGGGTAATTGGGCATTTAAGTTTCTTTGCAATTTTTTCCATTATTAACAAAATTAA(SEQ IDNO.994)

[2016] EGFRvIIIDprobe0995:TGAGCAACATTCTGCATAAGGTCTGTTTCCTCAGAATACGTTTCCCAAAGTGGAATCATCA

[2017] TGACGTAGAATTTAAGCATACTTACTTGTTTAAACAAATTGTCCAGTTGCTTCCCAAAA(SEQ IDNO.995)

[2018] EGFRvIIIDprobe0996:TGTTTTGTGAATTAAGATTTACATCAAGAATATGTAATGTTGTTACTGTCTCCCAAATACAG

[2019] GAtctttttctgaatataaaagttatacatgctaattgtagacaatGAAGGGTCATTA(SEQ IDNO.996)

[2020] EGFRvIIIDprobe0997:TCCTCATAGATAATGAAGTGCTTCTAATACTTGTGCTTTTATTCATTTATTCAAAAAGTGCT

[2021] AAATAAGCCCTGAAGGGGCTTTTGGGGGGTCATTTGGGGCTTATTTAGCACTTTTTGA(SEQ IDNO.997)

[2022] EGFRvIIIDprobe0998:ATAAATAAATAAAAGCACAAGTACAGTTTTTTTAAAATACTGTTTTCTATAATAGATTAATC

[2023] TTAAATGGCATGTTTTCCTTTATTTTACTGACAAAAGTTACTTACTCTGTGATTGAAT(SEQ IDNO.998)

[2024] EGFRvIIIDprobe0999:AATAAAAATTCTTTGGTTCAGCTGAGAGAAACTTGCAAGCTGACGTCCTTGATTATTTAA

[2025] AATGAAAGCAGCTGCCTGTTTTCATCTCTCTGCATCCTGAGGAAACTCTTCTGCAACGTG(SEQ IDNO.999)

[2026] EGFRvIIIDprobe1000:TTCCAGCCCTAGGTTCTAGCTGACCCTGTTCATCTGTTTGGCACGAGGGGCCCAACTAAC

[2027] ACTTGCGGCTACCTGGACGACAGCCAATCTAGTTGGAATGAGAGTTAGAGGCCATAGTCT(SEQ IDNO.1000)

[2028] EGFRvIIIDprobe1001:GTCAGCTGGGAAAGCAGCTTTTATTCCAAGGTGTGCCAACCGAAAGGCCACATGTTATTG

[2029] TCACAACCTGGTACCTACATCAGTGCTGACATCTTTAAGAACCTTAGAATTGGGAAATCA(SEQ IDNO.1001)

[2030] EGFRvIIIDprobe1002:GTTTAGCCCTATCTGCATGTGTAGCCGACAACCACACAATTGTTCCAACTTGAGGTTGCAT

[2031] TCAGAGCAACCTCATTTCCCCCATACTCCTGAGGAAAAGCAGACCAGAGACGCTGGGTC(SEQ IDNO.1002)

[2032] EGFRvIIIDprobe1003:AATCCAGAGTTATGGTTGGAAAAATGATGGAATAATTCTGCCCCTGGTGATAGGAGAGAG

[2033] GGACTCCATCTTGTCAACTGTCATGGTTCCCATGTGAAAGCTATCATTATCACTGAAATT(SEQ IDNO.1003)

[2034] EGFRvIIIDprobe1004:GAATGAGAACACAGAAGGGAAGAACAGGGAAATCCCCACAGAGTTAAAGAGGATGTGA

[2035] AGATTGCTTCATGTTTAATGTTTGTGTAAGTGCTTTGGGTTGGTTATGTGCTGTCTGAACAT(SEQ IDNO.1004)

[2036] EGFRvIIIDprobe1005:GTGCTCATTTCCATGGCTCATTGAGAGGGCAGACAGTCCAATGATACTCTTTAGAATCATT

[2037] CCCATGGGGAAGGAACAAAGAAGCCTGTAAAATAGAAATGCACATGTAAAAAGCATTGA(SEQ IDNO.1005)

[2038] EGFRvIIIDprobe1006:AGAAAGTGCCAGTGTATTGATTTTGGCCATGGTTTGTGCTCTACCACCTGGTTACTGTGAT

[2039] TGCAGAAGTGCCTTTGCAGATGAGGAAGAACCTGGCCAAGGCTCAATCCAACATCCAAA(SEQ IDNO.1006)

[2040] EGFRvIIIDprobe1007:GCCAGAGGCCATATTTCTTCACTCTTAAGATAATTTGGGTTCAAATTATAGTCCCTTTACAC

[2041] ACTCTCTGCCTCAAAAGGCCCAAGACTCTCTTTTGTTATGCTTGCCTAAACATGCCTT(SEQ IDNO.1007)

[2042] EGFRvIIIDprobe1008:TCAAAGAACTAGTTCTGTAAATACAACTTTATTATAAACCTCTCCTTTGCTTTTAAAAATG

[2043] GATCACCACGTCCATTTCTATGGTCCAACTTTGTCCCTTAATTTAAAATTTTTTCTTGG(SEQ IDNO.1008)

[2044] EGFRvIIIDprobe1009:ATTAAGTTTGATGCCTTGAAACATTAGGAACTCAAGCATACAAGATTGTATGCTGGTGGT

[2045] GAGGGAAGTAACTGTGCCTCCGCCTGTGCTGGGTGGATCAACATGGAGTGTGGACGAGCA(SEQ IDNO.1009)

[2046] EGFRvIIIDprobe1010:TAGGGATGTGTGGGTTTCTCACTAGCTGAGAGTGTTTTTAAATGTTGTATTTTGATGTTTG

[2047] TTATTTTCTGAATATTCTACAGTTAGACCTTTGATTTATTCTTTGATGCATTCATTTGA(SEQ IDNO.1010)

[2048] EGFRvIIIDprobe1011:ATAATATTTTTAATCTCCAGCCAGTTAGGTTTTTAATTTACACTTTTGTCCCTGATTTTAGGT

[2049] GTAGTGTTGTGTACACTACTGCCCAGTGTATGTTATGTTTGTAAACATTCATTGCAC(SEQ IDNO.1011)

[2050] EGFRvIIIDprobe1012:GCACAACAATGTGACTCACAATATTTTTGAGAAGTAAAAAGTTCATTATATAGTTATTAAC

[2051] TCAACCCTACAGTTATATTCGTGAAATACCTTGTGAAATTTATTTTTTGCCTACTGGAG(SEQ IDNO.1012)

[2052] EGFRvIIIDprobe1013:CTCTTACAGGTTAATCCTGTCTTCAAGATTTTCATAGAATTTTCATCTACCACCCACCCCTT

[2053] TAAATTTCAACATTTTTTTATTTTGGCATTTTAATGCAATTCAATGCATTATAGGGAC(SEQ IDNO.1013)

[2054] EGFRvIIIDprobe1014:AAGCTATCTCTTATTATGAATTGCACCTTATATAAACTTAAAGATCTTTTATCACAAATTTCT

[2055] TTGCTGTGTCCTTTAGTGAGAATTTGTATTATCAGTCACTAAAGCTCACTAAGTTAG(SEQ IDNO.1014)

[2056] EGFRvIIIDprobe1015:TAAGCTTTGCGCCCAGATGACCTGGGCAGGAATGGGTGAGTCTCTGTGTGGAGAGAGTG

[2057] AAGAAACTGCTACCCTTAATACCTGGACCTTGAGGGATTGTTTTATTTTAGTTTTTCTGCA(SEQ IDNO.1015)

[2058] EGFRvIIIDprobe1016:TTTCTCAGTATTTCATGTGATATCTGTCTTTTTCTTCCAGTTTGCCAAGGCACGAGTAACA

[2059] AGCTCACGCAGTTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATGTTCAAT(SEQ IDNO.1016)

[2060] EGFRvIIIDprobe1017:GTGAGTCCTCCTCTGTGGGCCCTCTAACTGGTCAGGCATCCTTGTCCCGCTCTGTCTCCT

[2061] GCTGAGCCCTGGAGTATCCCATCTTGGAGAGTCTTTGGGTGGATGTGTTTGCCTTGCTTG(SEQ IDNO.1017)

[2062] EGFRvIIIDprobe1018:GAGGAGGCGACCCTGTGCCCGTCCAGGCACACAGGCGAGGGGAGGGGCTGGCTTGCTA

[2063] CCGAGGAGCGGGCAGGTGGTGGCCATCTCCACCCATGGGGGCTGCTCAGTGCACAGGGCAGA(SEQ ID

[2064] NO.1018)

[2065] EGFRvIIIDprobe1019:TCTGGGTGGCCAGGCCACCTCACAGGAGAAACACCTGCTGCTCAGCCCTCACCACTCAT

[2066] CCAGCAGCCACAGCCGTGGGTATTCAGTTGTCTGCTGGGCACAAAGCCGTGGGCATGCCAC(SEQ IDNO.1019)

[2067] EGFRvIIIDprobe1020:TGTTTAGTGCTTGTGCCAAGCAGGTATTTAATACACCGAAATCAGAGAGTCTATCAGAAG

[2068] ACCTGCCTTCTTGAGTGGTTAAAATTCTAGTGAAAGTTATGCCTCTTAGGAGTATTGCAG(SEQ IDNO.1020)

[2069] EGFRvIIIDprobe1021:AGGttttgtttttgtttttattttgttttgttttaatggttggtttgagtttgctttgtgtgtcttacatttgtGTGGTGGCTCCAGGGTTT AGGGAAATTGTGACATAAAATAAT(SEQID NO.1021)

[2070] EGFRvIIIDprobe1022:TCCTGACAGAGAAAGCAAAACTTTGTCTAATGAAAGAGTTTTAGAAGCCACTCTTGATCT CTAGAAGGGGATTAACTGAAAAAAATTGAAAGAACAATTATGAGGGGAGATTTTTT(SEQID NO.1022)EGFRvIIIDprobe1023:ACCCTGCCAGATTTGTGTACATGAAAAATTTTACATTCCGTATGGAAAAAAAAAACAA AATAATAAGCCATTAAGGTAAATGAAACAAAGCTAAAGAAAAATGTGCCACAGTGA(SEQ ID NO.1023)EGFRvIIIDprobe1024:TGACACAGATATATCTTTGAGATAGGGCTTAACAGAGCTTTAAAATCCATAGGAAAACAC TTCGAGCCTGAGATACCAAGAGCAGATGGTTCACAGAAGAATCAATGTCCTATAAAT(SEQ ID NO.1024)EGFRvIIIDprobe1025:TCACAGAAGAATCATCAATGTCCTATAAAATTTTTGAGGATCTTCTTGGGGAACTTAAAA CAGGAACAGGCCAGGCACAGTGGCTCATtggctcatgccttaatcccagcactttggg(SEQ ID NO.1025)

[2071] EGFRvIIIDprobe1026:ggtgacagagcaagactccatctcagacaaaaaaaaaaGGAAGACATAGAGCTCCTAAAAATAACGCAGAAG TCTGCTATTAATACAAATGAATTACTTTAAAGGTGAGAGCAGGTGGAG(SEQID NO.1026)

[2072] EGFRvIIIDprobe1027:TAAAAATAACGCAGAAGTCTGCTTAATACAAATGAATTACTTTAAAGGTGAGAGCAGG TGGAGGAGAGGGCTGAGGTGCCTGCTGGGACGCAAAACAGCTGGCCCCTCAAGGGACCCA(SEQID NO.1027)EGFRvIIIDprobe1028:GTGTTTCCTGCCATGATGAAACACCTGTATTGTCCACATTGCGGCCTAGAATGTTATTAAA CTCTTGAACGGGATTCCTTCTCTATTTGCAACCTTTCATTCTTTGTCCTTAAAGTAAAT(SEQ ID NO.1028)EGFRvIIIDprobe1029:AAAGCCAAAGGAGGATGGAGCCTTTCCATCACCCTCAAGAGGACCTGGACCGCCTGTG TGAGGCCCGAGCACCTGGTGCCACCGTCATCACCTTCCTTTCATGCTCTTCCCCAGGTA(SEQ ID NO.1029)EGFRvIIIDprobe1030:GGTAATTATGTGGTGACAGATCAGGCTCGTGCGTCCGAGCCTGTGGGCCGACAGCTAT GAGATGGAGGAAGACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGGT(SEQ ID NO.1030)

[2073] EGFRvIIIDprobe1031:CCTCACTTGCCCAGCGTGTCCTCTCTCCTCCATAGGTCTGCCGCAAATTCCGAGACG AAGCCACGTGCAAGGACACCTGCCCCCCACTCATGCTCTACAACCCCACCAGTACCAGATGGA(SEQID NO.1031)

[2074] EGFRvIIIDprobe1032:TGCTCTACAACCCCACCACCGTACCAGATGGATGTGAACCCCGAGGGCAAATACAGC TTTGGTGCCACCTGCGTGAAGAAGTCCCCGTGAGTCCTCCTGTGGGCCCTCTAACTGGTCA(SEQID NO.1032)

[2075] 2. Experimental methods

[2076] 1) DNA library construction

[2077] Step 1. DNA fragmentation: Pipette 200 ng of clinical sample DNA into a 0.2 mL PCR tube, make up to 7 μL with nuclease-free water, and place on ice. Add 2 μL of 5× fragmentation buffer and 1 μL of fragmentation enzyme to each tube, vortex to mix, and centrifuge briefly. Place the reaction system in a PCR instrument and run the following program: 37°C for 10 min; 65°C for 5 min; 4°C hold. After the program is complete, remove the tubes and place them on ice. Add 40 μL of nuclease-free water to each tube.

[2078] Step 2. End Repair and Adding "A": Add 16 μL of enzyme reaction buffer and 4 μL of enzyme mix to the fragmented DNA product, vortex to mix, and centrifuge briefly. Place the reaction system in a PCR instrument and run the following program: heated lid at 105°C; 20°C for 15 minutes; 72°C for 15 minutes; 4°C hold; 70 μL of the system.

[2079] Step 3. Prepare the adapter ligation reagent: Add 23 μL of ligation buffer and 2 μL of ligase to a new 0.2 mL PCR tube, pipette to mix, centrifuge briefly, and equilibrate at room temperature for 30-45 minutes.

[2080] Step 4. Adapter ligation: Add 25 μL of ligation reaction system premix to the end-repair and A-tailing product, vortex to mix, and centrifuge instantly; then add 5 μL of DNA adapter, vortex to mix, and centrifuge instantly; place the adapter ligation reaction system in a PCR instrument and run the program: hot cover 70℃; 20℃ 30min; 4℃ Hold; system 100 μL.

[2081] Step 5. Post-ligation magnetic bead purification: Add 80 μL (0.8×) of AMPure XP magnetic beads to the adapter ligation product for purification, wash twice with 180 μL of 80% ethanol, and discard the supernatant. After drying at room temperature, resuspend in 35 μL of nuclease-free water. After magnetic separation, aspirate 34 μL of the supernatant into a new 0.2 mL PCR tube.

[2082] Step 6. Library pre-amplification: Add 10 μL of 5× PCR buffer and 1 μL of PCR polymerase to each purified ligation product, shake and mix, and centrifuge instantly. Then add 5 μL of the corresponding double-ended tag primer pair, shake and mix, and centrifuge instantly. Place the reaction system in a PCR instrument and run the program: hot cover 105°C; 98°C for 2 minutes; (98°C for 30 seconds, 60°C for 30 seconds, 72°C for 1 minute; 12 cycles); 72°C for 5 minutes; 4°C Hold; system 50 μL.

[2083] Step 7. Post-amplification magnetic bead purification: Add 50 μL (1.0×) of AMPure XP magnetic beads (Beckman Coulter, Catalog No. A63882) to the PCR reaction system for purification. Wash twice with 180 μL of 80% ethanol and discard the supernatant. After drying at room temperature, resuspend in 22 μL of nuclease-free water. After magnetic separation, pipette 20 μL of the supernatant into a new 0.2 ml PCR tube to obtain the DNA sample intermediate library.

[2084] Step 8. Intermediate library quality control: Determine the intermediate library concentration using a Qubit 3.0 fluorometer and analyze the library fragment distribution using an Agilent 2100. The total library volume must be ≥ 500 ng, and the main peak of the library fragment distribution must be between 250 and 500 bp.

[2085] 2) Probe hybridization capture

[2086] Step 1. Intermediate library mixing and concentration: Take 500 ng of each intermediate library and add it to a 1.5 mL centrifuge tube for mixing. Then add 10 μL Cot DNA and 2 μL universal blocking sequence. Vortex and mix thoroughly, centrifuge briefly, and place the mixture in a concentrator at 60°C to concentrate it to a dry powder.

[2087] Step 2. Hybridization reaction: Add 10 μL of hybridization reaction solution 1, 2 μL of hybridization reaction solution 2, 2 μL of DNA probe, and 2 μL of nuclease-free water to the concentrated sample tube, mix thoroughly, and transfer to a new 0.2 mL PCR tube. Incubate at room temperature for 10 min. After incubation, place the tube in a PCR instrument and incubate at 95°C for 5 min; then incubate at 65°C for 2 h.

[2088] Step 3. Washing of Streptavidin Magnetic Beads: Take 50 μL of streptavidin magnetic beads and wash them twice with 100 μL of 1× magnetic bead washing buffer, discard the supernatant; finally, add 50 μL of 1× magnetic bead washing buffer to resuspend and transfer them to a new 0.2 mL PCR tube.

[2089] Step 4. Capture reaction: Transfer all 16 μL of hybridization products to the PCR tube containing capture magnetic beads, mix thoroughly, and incubate at 65°C for 30 minutes to perform the capture reaction.

[2090] Step 5. Post-capture washing: Wash at 65°C: Add 120 μL of 1× Hybridization Wash Solution 1 preheated at 65°C to each tube for washing, and discard the supernatant by magnetic aspiration; then add 150 μL of 1× Hybridization Wash Solution 4 preheated at 65°C for washing, and discard the supernatant by magnetic aspiration; repeat the process once, adding 150 μL of 1× Hybridization Wash Solution 4 preheated at 65°C for washing, and discard the supernatant by magnetic aspiration.

[2091] Wash at room temperature: Add 150 μL of room temperature 1× Hybridization Wash Buffer 1 for washing, magnetically aspirate and discard the supernatant; add 150 μL of room temperature 1× Hybridization Wash Buffer 2 for washing, magnetically aspirate and discard the supernatant; add 150 μL of room temperature 1× Hybridization Wash Buffer 3 for washing, transfer the system to a new PCR tube with the corresponding number, magnetically aspirate and discard the supernatant; add 23 μL of nuclease-free water to resuspend.

[2092] Step 6. Post-capture library amplification: Add 25 μL of 2× PCR reaction mixture and 2 μL of P5&P7 primer mixture to the captured sample, vortex and centrifuge, place on the PCR instrument, and run the program: hot cover 105°C; 98°C 45s; (98°C 15s, 57°C 30s, 72°C 30s; 10 cycles); 72°C 1min; 4°C Hold; system 50 μL.

[2093] Step 7. Post-amplification magnetic bead purification: Add 50 μL (1.0×) of AMPure XP magnetic beads to the PCR reaction product for purification. Wash twice with 180 μL of 80% ethanol. After drying at room temperature, resuspend in 25 μL of nuclease-free water. After magnetic separation, pipette 23 μL of the supernatant into a newly labeled 1.5 mL centrifuge tube to obtain the captured library.

[2094] Step 8. Post-capture library quality control: Determine the post-capture library concentration using a Qubit 3.0 fluorometer and analyze the library fragment distribution using an Agilent 2100. The library concentration must be ≥ 3 nM, and the main peak of the library fragment distribution must be between 250 and 500 bp.

[2095] 3. Experimental results

[2096] The total amount of intermediate libraries constructed for each sample was >500 ng, and the main peak of library fragment distribution was 250-500 bp; the concentration of the library after capture was >3 nM, and the main peak of library fragment distribution was 250-500 bp.

[2097] Step 2 RNA library construction and probe hybridization capture

[2098] 1. Experimental Materials

[2099] Samples: RNA from 6 clinical samples with known EGFRvIII mutation-positive RNA levels;

[2100] Library construction reagents: A commercial RNA library construction kit specifically developed for the Illumina high-throughput sequencing platform; Dual-end UDI index adapters: Commercial adapters with 8nt dual-end unique sample indexes compatible with the Illumina high-throughput sequencing platform;

[2101] Hybrid capture reagents: Commercially available Sequencing platform RNA library hybridization capture kit;

[2102] Hybridization capture probe: e.g. Figure 1 The RNA level probe pool designed and synthesized for detecting EGFRvIII mutation at the RNA level is shown;

[2103] The RNA level sequence information is as follows:

[2104] EGFRvIIIRprobe1:GCGTCCGCCCGAGTCCCCGCCTCGCCGCCAACGCCACAACCACCGCGCACGGCCCCCTGAC TCCGTCCAGTATTGATCGGGAGAGCCGGAGCGAGCTCTTCGGGGAGCAGCGATGCGACC (SEQ IDNO.1033) EGFRvIIIRprobe2: GAGAGCCGGAGCGAGCTCTTCGGGGAGCAGCGATGCGACCCTCCGGGACGGCCGGGGCAG CGCTCCTGGCGCTGCTGGCTGCGCTCTGCCCGGCGAGTCGGGCTCTGGAGGAAAAGAAAG (SEQ IDNO.1034) EGFRvIIIRprobe3: GTAATTATGTGGTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGCTATGAG ATGGAGGAAGACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGTGT (SEQ IDNO.1035) EGFRvIIIRprobe4: CGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGTGTGTAACGGAATAGGTATTGGTG AATTTAAAGACTCACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACC (SEQ IDNO.1036) EGFRvIIIRprobe5: GCGCTCCTGGCGCTGCTGGCTGCGCTCTGCCCGGCGAGTCGGGCTCTGGAGGAAAAGAAAG GTAATTATGTGGTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGCTAT (SEQ IDNO.1037)

[2105] 2. Experimental methods

[2106] 1) RNA library construction

[2107] Step 1. RNA fragmentation: Use an RNase-free pipette tip to draw up 200 ng of RNA-positive sample and add it to a 0.2 mL PCR tube. Fill up to 10 μL with nuclease-free water and place on ice. Add 10 μL of 2× fragmentation buffer to each sample tube, vortex to mix, and centrifuge instantly. Place the fragmentation reaction system in a PCR instrument and run the following program: heated lid 105°C; 94°C for 2 min; 4°C for 1 min; 4°C hold; system 20 μL.

[2108] Step 2. First-strand cDNA synthesis: Add 8.5 μL of first-strand synthesis reagent to the fragmented product, vortex to mix, and centrifuge immediately. Place the first-strand synthesis reaction system in a PCR instrument and run the following program: heated lid at 70°C; 25°C for 10 min; 37°C for 40 min; 4°C hold; system volume 28.5 μL.

[2109] Step 3. Second-strand cDNA synthesis: Add 25 μL of second-strand synthase and 5 μL of random primers to the first-strand synthesis product, vortex and mix, and centrifuge immediately. Place the second-strand synthesis reaction system in a PCR instrument and run the program: heated lid 70°C; hold at 4°C; system 58.5 μL.

[2110] Step 4. cDNA magnetic bead purification: After the second-strand synthesis reaction is completed, add 105 μL (1.8×) AMPure XP magnetic beads to the reaction system for purification. Wash twice with 180 μL 80% ethanol and discard the supernatant. After drying at room temperature, add 52 μL nuclease-free water to resuspend. After magnetic separation, aspirate 50 μL of supernatant into a new 0.2 mL PCR tube.

[2111] Step 5. End Repair and Adding A: Add 16 μL of enzyme reaction buffer and 4 μL of enzyme mix to the double-stranded cDNA product, vortex and centrifuge. Place the reaction system in a PCR instrument and run the following program: heated lid at 105°C; 20°C for 15 minutes; 72°C for 15 minutes; 4°C hold; 70 μL of the system.

[2112] Step 6. Prepare the adapter ligation reagent: Add 23 μL of ligation buffer and 2 μL of ligase to a new 0.2 mL PCR tube, pipette to mix, centrifuge briefly, and equilibrate at room temperature for 30-45 minutes.

[2113] Step 7. Adapter ligation: Add 25 μL of ligation reaction system premix to the end-repair and A-tailing product, vortex to mix, and centrifuge instantly; then add 5 μL of RNA adapter, vortex to mix, and centrifuge instantly; place the adapter ligation reaction system in a PCR instrument and run the program: hot cover 70℃; 20℃ 30min; 4℃ Hold; system 100 μL.

[2114] Step 8. Post-ligation magnetic bead purification: Add 80 μL (0.8×) of AMPure XP magnetic beads to the adapter ligation product for purification, wash twice with 180 μL of 80% ethanol, and discard the supernatant. After drying at room temperature, resuspend in 35 μL of nuclease-free water. After magnetic separation, aspirate 34 μL of the supernatant into a new 0.2 mL PCR tube.

[2115] Step 9. Library Pre-amplification: Add 10 μL of 5× PCR buffer and 1 μL of PCR polymerase to the purified ligation product, vortex to mix, and centrifuge. Add 5 μL of the corresponding double-end indexed primer pair, vortex to mix, and centrifuge. Place the reaction system in a PCR instrument and run the following program: heated lid at 105°C; 98°C for 2 minutes; (12 cycles of 98°C for 30 seconds, 60°C for 30 seconds, and 72°C for 1 minute); 72°C for 5 minutes; 4°C hold; 50 μL of the system.

[2116] Step 10. Post-amplification magnetic bead purification: Add 50 μL (1.0×) AMPure XP magnetic beads to the PCR reaction system for purification. Wash twice with 180 μL 80% ethanol and discard the supernatant. After drying at room temperature, resuspend in 22 μL nuclease-free water. After magnetic separation, pipette 20 μL of the supernatant into a new 0.2 ml PCR tube to obtain the RNA sample intermediate library.

[2117] Step 11. Intermediate library quality control: Determine the intermediate library concentration using a Qubit 3.0 fluorometer and analyze the library fragment distribution using an Agilent 2100. The total library volume must be ≥ 500 ng, and the main peak of the library fragment distribution must be between 250 and 500 bp.

[2118] 2) Probe hybridization capture

[2119] Step 1. Intermediate library mixing and concentration: Take 500 ng of each intermediate library and add it to a 1.5 mL centrifuge tube for mixing. Then add 10 μL Cot DNA and 2 μL universal blocking sequence. Vortex and mix thoroughly, centrifuge briefly, and place the mixture in a concentrator at 60°C to concentrate it to a dry powder.

[2120] Step 2. Hybridization Reaction: Add 10 μL of Hybridization Reaction Solution 1, 2 μL of Hybridization Reaction Solution 2, 2 μL of RNA probe, and 2 μL of nuclease-free water to the concentrated sample. Mix thoroughly and transfer to a new 0.2 mL PCR tube. Incubate at room temperature for 10 minutes. After incubation, place the tube in a PCR instrument and incubate at 95°C for 5 minutes, followed by incubation at 65°C for 2 hours.

[2121] Step 3. Washing of streptavidin magnetic beads: Take 50 μL of streptavidin magnetic beads, wash twice with 100 μL of 1× magnetic bead washing solution, and discard the supernatant; finally, add 50 μL of 1× magnetic bead washing solution and pipette until all the magnetic beads are resuspended, and transfer to a new 0.2 mL PCR tube.

[2122] Step 4. Capture reaction: Transfer all 16 μL of hybridization products to the PCR tube containing capture magnetic beads, mix thoroughly, and incubate at 65°C for 30 minutes to perform the capture reaction.

[2123] Step 5. Post-capture washing: Wash at 65°C: Add 120 μL of 1× Hybridization Wash Solution 1 preheated at 65°C to each tube for washing, aspirate magnetically and discard the supernatant; then add 150 μL of 1× Hybridization Wash Solution 4 preheated at 65°C for washing, aspirate magnetically and discard the supernatant; repeat this process once, add 150 μL of 1× Hybridization Wash Solution 4 preheated at 65°C for washing, aspirate magnetically and discard the supernatant.

[2124] Wash at room temperature: Add 150 μL of room temperature 1× Hybridization Wash Buffer 1 for washing, magnetically aspirate and discard the supernatant; add 150 μL of room temperature 1× Hybridization Wash Buffer 2 for washing, magnetically aspirate and discard the supernatant; add 150 μL of room temperature 1× Hybridization Wash Buffer 3 for washing, transfer the system to a new PCR tube with the corresponding number, magnetically aspirate and discard the supernatant; add 23 μL of nuclease-free water to resuspend.

[2125] Step 6. Post-capture library amplification: Add 25 μL of 2× PCR reaction mixture and 2 μL of P5&P7 primer mixture to the captured sample, vortex and centrifuge, place on the PCR instrument, and run the program: hot cover 105°C; 98°C 45s; (98°C 15s, 57°C 30s, 72°C 30s; 10 cycles); 72°C 1min; 4°C Hold; system 50 μL.

[2126] Step 7. Post-amplification magnetic bead purification: Add 50 μL (1.0×) AMPure XP magnetic beads to the PCR reaction product for purification, wash twice with 180 μL 80% ethanol, and discard the supernatant. After drying at room temperature, resuspend in 25 μL nuclease-free water. After magnetic separation, pipette 23 μL of the supernatant into a new labeled 1.5 mL centrifuge tube to obtain the captured library.

[2127] Step 8. Post-capture library quality control: Determine the post-capture library concentration using a Qubit 3.0 fluorometer and analyze the library fragment distribution using an Agilent 2100. The library concentration must be ≥ 3 nM, and the main peak of the library fragment distribution must be between 250 and 500 bp.

[2128] 3. Experimental results

[2129] The total amount of intermediate libraries constructed for each sample was >500 ng, and the main peak of library fragment distribution was 250-500 bp; the concentration of the library after capture was >3 nM, and the main peak of library fragment distribution was 250-500 bp.

[2130] Step 3: Library sequencing and data analysis

[2131] 1. Analysis Method

[2132] EGFRvIII has a large deletion of exons 2-7 at the DNA level. GeneFuse software was used to scan DNA sequencing data and compare it with the hg19 human reference genome to obtain large-scale variants of the EGFR gene. If the breakpoints of the large-scale variant were within intron 1 and exon 7 to intron 7, respectively, and if at least four sequencing reads supported the variant, the test was considered positive for EGFRvIII.

[2133] At the RNA level, EGFRvIII has breakpoints mostly occurring in exon 1 and exon 8 because the introns are cut out. The bioinformatics analysis method is as follows: First, use sentieon STAR to align the RNA sequencing data to the hg19 version of the human reference genome to obtain the alignment information of each sequencing read. Because the PE150 sequencing strategy is used, the same RNA molecule fragment will be sequenced from both ends to obtain two reads, R1 and R2. The two reads are merged into a complete read based on their alignment positions. Then, the merged reads are classified according to their alignment information:

[2134] 1) If a read segment is soft-truncated, the front end of the read segment can be correctly aligned to exon 1 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 8. The read segment is recorded as m and the breakpoint is recorded as M.

[2135] 2) Similarly, if there is soft truncation in the read segment, the front end of the read segment can be correctly aligned to exon 8 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 1. The read segment is recorded as n and the breakpoint is recorded as N.

[2136] 3) The total number of sequencing reads is recorded as s.

[2137] Reads classified as 1) and 2) are reads supporting the EGFRvIII mutation. Because the number of these reads is affected by the EGFR gene expression level and the total sequencing data volume, even for the same sample, the number of supporting reads obtained from different sequencing data volumes will also be different. In order to make the number of supporting reads more representative of the accuracy of exon mutations, the number of supporting reads needs to be normalized. The normalized value is the number of variant supporting reads per megabyte of sequencing reads, calculated as follows:

[2138] Number of variant support reads per megabyte of sequencing reads = ((m+n)*1000000) / s

[2139] If the number of variant support reads per megabyte of sequencing reads is greater than or equal to 0.2, the sample is judged as positive, otherwise it is judged as negative.

[2140] 2. Experimental results

[2141] This method was used to test six samples with known RNA-based EGFRvIII mutations. Three samples tested positive for both DNA and RNA, and three tested positive only for RNA. The results are shown in Table 1.

[2142] Table 1 DNA level and RNA level detection results

[2143]

[2144] These results indicate that this assay can effectively detect EGFRvIII mutations at both the DNA and RNA levels. RNA detection is more sensitive than DNA detection.

[2145] Example 2 Comparison of RNA-level EGFRvIII mutation detection results with Sanger detection results

[2146] For 36 clinical FFPE samples of glioma with existing Sanger RNA-based EGFRvIII mutation detection results, library construction and sequencing data analysis were performed using the same method as described in step 2 of Example 1. The RNA-based EGFRvIII mutation detection probe set and detection method were compared with the Sanger RNA-based EGFRvIII mutation detection results, as shown in Table 2.

[2147] Table 2 Comparison of RNA level detection results and Sanger detection results

[2148]

[2149] The probe set detection method provided in this example has a consistency rate of 94.44% compared to Sanger results, and the positive detection rate of this method for EGFRvIII mutation is higher than that of Sanger detection.

[2150] The above examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. Those skilled in the art can make several simple deductions, modifications or substitutions based on the concept of the present invention.

Claims

1. A probe set for detecting EGFRvIII mutation at the DNA level, characterized in that: The probe group comprises any one of the probes with nucleotide sequences as shown in SEQ ID NO.1-1032, or a probe with a homology of more than 70% with the sequence shown in SEQ ID NO.1-1032.

2. A probe set for detecting EGFRvIII mutation at RNA level, characterized in that: The probe group comprises any one of the probes with nucleotide sequences as shown in SEQ ID NOs. 1033 to 1037, or probes with 70% or more homology to the sequences shown in SEQ ID NOs. 1033 to 1037.

3. A kit for detecting EGFRvIII mutation, characterized in that: The kit comprises the probe set according to claim 1 and / or claim 2.

4. The kit according to claim 3, characterized in that The kit also comprises any one or a combination of at least two of a DNA fragmentation reagent, an RNA fragmentation reagent, a cDNA single-strand synthesis reagent, a cDNA double-strand synthesis primer, a cDNA double-strand synthesis reagent, a linker connection reagent, a hybridization capture reagent, a hybridization product amplification reagent or streptavidin affinity magnetic beads.

5. A method for preparing a library for detecting EGFRvIII mutations, characterized in that: The method comprises performing a mixed reaction on a DNA and / or RNA intermediate library obtained from a sample to be tested using the probe set according to claim 1 or 2 to obtain a library for detecting EGFRvIII mutations; Preferably, the DNA and / or RNA intermediate library comprises DNA and / or RNA molecules that have been subjected to fragmentation, end repair, end "A" addition reaction, adapter ligation, and PCR amplification in sequence.

6. A method for detecting EGFRvIII mutation at the DNA level, characterized in that: The method comprises performing probe hybridization capture on a DNA intermediate library obtained from a sample to be tested using the probe set of claim 1; Preferably, the step of obtaining a DNA intermediate library from the sample to be tested comprises extracting DNA from the sample to be tested, fragmenting the sample DNA, performing end repair, end "A" reaction, linker ligation, PCR amplification and purification on the fragmented DNA, and then using Qubit quantification and 2100 quality control detection to obtain the DNA intermediate library.

7. The method according to claim 6, characterized in that The method also includes the steps of library amplification and purification, sequencing and analysis of sequencing results after probe hybridization capture; Preferably, the sequencing includes sequencing through a sequencing platform to obtain the original data of the sample to be tested; preferably, the sequencing is performed by an Illumina platform in a PE150 mode; Preferably, the step of analyzing the sequencing results includes comparing the original offline data of the sample to be tested with the hg19 version of the human reference genome to obtain the large-fragment variation results of the EGFR gene. If the breakpoints of the large-fragment variation belong to intron 1 and exon 7 to intron 7, respectively, and no less than 4 sequencing reads support the number of variation reads, it is judged to be EGFRvIII positive.

8. A method for detecting EGFRvIII mutation at RNA level, characterized in that: The method comprises performing probe hybridization capture on an RNA intermediate library obtained from a sample to be tested using the probe set of claim 2; Preferably, the step of obtaining the RNA intermediate library from the sample to be tested includes extracting RNA from the sample, fragmenting the sample RNA, and sequentially subjecting the fragmented RNA to single-strand cDNA synthesis, second-strand cDNA synthesis, end repair, end "A" reaction, and adapter ligation, PCR amplification and purification, and then using Qubit quantification and 2100 quality control detection to obtain the RNA intermediate library.

9. The method according to claim 8, characterized in that The method also includes the steps of library amplification and purification, sequencing and analysis of sequencing results after probe hybridization capture; Preferably, the sequencing is performed by Illumina platform in PE150 mode; Preferably, the step of analyzing the sequencing results includes aligning the captured RNA molecule sequencing data to the hg19 version of the human reference genome to obtain the alignment information of each sequencing read segment; merging the alignment positions of the two read segments R1 and R2 sequenced from both ends of the same RNA molecule fragment into a complete read segment; and classifying according to the alignment information of the merged read segments: 1) If there is soft truncation in the read segment, the front end of the read segment can be correctly aligned to exon 1 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 8. The read segment is recorded as m and the breakpoint is recorded as M; 2) If there is soft truncation in the read segment, the front end of the read segment can be correctly aligned to exon 8 of the reference genome sequence, and the back end of the read segment can be correctly aligned to exon 1. The read segment is recorded as n and the breakpoint is recorded as N; 3) The total number of sequencing reads is recorded as s; The reads classified as 1) and 2) are the reads supporting the EGFRvIII mutation. The number of supporting reads is then normalized to the number of variant supporting reads per megabyte of sequencing reads, calculated as follows: Number of variant support reads per megabyte of sequencing reads = ((m+n)*1000000) / s; If the number of variant support reads per megabyte of sequencing reads in the sample is greater than or equal to 0.2, it is judged as positive, otherwise it is judged as negative.

10. The method according to claim 5, 6 or 8, characterized in that: The sample to be tested includes tumor cells, blood, plasma, exosome solution, pleural effusion, peritoneal effusion, saliva, urine or tissue.

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