Method for collecting juvenile sea urchins by using allicin

Abstract

The invention discloses a method for collecting juvenile sea urchins by using allicin, which comprises the following steps: soaking a substratum to which juvenile sea urchins are attached in an allicin solution for 0.5-1.5 minutes, and then collecting the juvenile sea urchins which are autonomously stripped from the substratum; the stripping rate of the juvenile sea urchins on the substratum is larger than or equal to 90%, and the survival rate of the juvenile sea urchins automatically stripped from the substratum is larger than or equal to 88%. According to the method, the juvenile sea urchins are stimulated to be autonomously stripped through the biological activity of the allicin, physical damage to the juvenile sea urchins caused by a traditional mechanical stripping method is effectively avoided, and the survival rate of the juvenile sea urchins after stripping is remarkably increased. Moreover, the source of the allicin is wide, the extraction process is relatively simple, the cost is relatively low, and compared with some special chemical stripping agents or biological agents, the method is more suitable for large-scale sea urchin culture production application.

Description

Technical Field

[0001] The invention relates to the field of sea urchin breeding, in particular to a method for collecting young sea urchins by utilizing allicin. Background Art

[0002] During the early development of sea urchins, the fertilized egg undergoes multiple stages and eventually metamorphoses into a juvenile gall. This process includes the fertilized egg, cleavage stage, blastocyst stage, rotating blastocyst stage, gastrula stage, prismatic larvae, two-armed larvae, four-armed larvae, six-armed larvae, eight-armed larvae, until it metamorphoses into a juvenile gall.

[0003] Juvenile gall refers to the growth period of sea urchins from metamorphosis to maturity. Cultivation at this stage is of great significance to the sea urchin aquaculture industry. The key to juvenile gall cultivation is to provide a suitable growth environment, adequate nutrition and disease prevention. After metamorphosis, juvenile galls begin to feed on benthic diatoms, the ciliary activity on the ciliary belt gradually weakens, the tube feet begin to extend, and the shell diameter of juvenile galls gradually increases during the juvenile gall cultivation process. Therefore, the growth rate and survival rate at this stage are important indicators for evaluating the effect of juvenile gall cultivation. However, the juvenile gall cultivation process often has a high mortality rate due to water quality or aquaculture management. Especially when the juvenile galls are transferred from the corrugated board to the aquaculture pond with denser benthic diatoms, the juvenile gall tube feet will be destroyed by human factors such as traditional mechanical stripping methods, resulting in the death of many juvenile gall larvae. Collecting juvenile galls after metamorphosis during the attachment period is an important part of the sea urchin aquaculture process. Therefore, a more gentle, efficient and environmentally friendly method for stripping juvenile sea urchins is urgently needed. Allicin can react with the sulfhydryl (-SH) of biological molecules to destroy the structure and function of proteins and enzymes. Summary of the invention

[0004] The purpose of the present invention is to provide a method for collecting young sea urchins by using allicin, so as to solve the problem that the traditional mechanical stripping method causes mechanical damage to the young sea urchins, thus affecting their survival rate and growth and development.

[0005] The present invention provides a method for collecting young sea urchins by using allicin, comprising soaking a substrate with young sea urchins attached in an allicin solution, soaking for 0.5-1.5 minutes, and collecting young sea urchins peeled off from the substrate by a sieve; the peeling rate of young sea urchins on the substrate is greater than or equal to 90%, and the survival rate of young sea urchins peeled off from the substrate is greater than or equal to 88%; The enzymes in the tube foot cells of the young sea urchins that were detached from the substrate include superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX) and catalase (CAT). Allicin is an organic sulfur compound extracted from the bulb of garlic, a plant of the Allium family. Its scientific name is diallyl thiosulfinate, and its chemical formula is C6H 10 OS2.

[0006] Furthermore, in the method of the present invention, the superoxide dismutase content in the tube foot cells of the juvenile sea urchins that are peeled off from the attachment substrate is in the range of 6000-14000 U / mgprot, the malondialdehyde content is in the range of 80-320 nmol / mgprot, the glutathione peroxidase content is in the range of 5000-10000 U / mgprot, and the catalase content is in the range of 30-80 U / mgprot.

[0007] The contents of SOD, MDA, GPx and CAT in the podocytes of young sea urchins must be appropriate, representing the young sea urchin's immune ability and resistance to environmental stress. If it is lower or higher than this range, it proves that the young sea urchin is under stress from the environment and causes physiological discomfort, which may even lead to the death of the young sea urchin. This patent uses these four indicators to illustrate the range of the young sea urchin's resistance to allicin concentration.

[0008] Furthermore, the mass concentration of allicin in the allicin solution in the method of the present invention is 1-20 mg / L.

[0009] Furthermore, in the method of the present invention, the site of action of the allicin is the tube feet extended by the young sea urchin.

[0010] Furthermore, in the method of the present invention, the density of immature gallbladders in the allicin solution is 30-50 / mL.

[0011] The concentration of the allicin solution and the soaking time must be appropriate. If the concentration of the allicin solution is too high, too much irritant will cause the death of the young sea urchins. If the concentration of the allicin solution is too low, the expected effect of self-peeling cannot be achieved. In the present invention, the action time of the allicin solution on the young sea urchins adsorbed on the attachment base is 0.5-1.5 min, and the action time must also meet the above range. If the action time exceeds 1.5 min, the activity of the young sea urchins will decrease. On the contrary, if the action time is too short, the effect of self-peeling cannot be achieved.

[0012] Furthermore, the method of the present invention specifically comprises the following steps: 1) Pretreatment of juvenile sea urchins The substrate with the young sea urchins adsorbed is placed in a temporary culture pond, and the temporary culture environment includes: using natural seawater, the water temperature is 22-24 ℃, the salinity is 28-30‰, the dissolved oxygen content is 5.5-6.0 mg / L, and the temporary culture time is 12-24 hours; 2) Treatment of the substrate with juvenile sea urchins Taking out the attachment base with the young sea urchins adsorbed from the temporary culture pond, and then immersing the attachment base with the young sea urchins adsorbed in an allicin solution with a mass concentration of 1-20 mg / L of allicin, and controlling the immersion time to be 0.5-1.5 min; 3) Collecting juvenile sea urchins After the soaking is completed, the juvenile sea urchins that have peeled off by themselves are collected using a sieve, and the collected juvenile sea urchins are cleaned and temporarily cultured.

[0013] Furthermore, in the method of the present invention, the diameter of the sieve is less than 100 meshes, and a mesh that is too large may cause the immature gall to leak out from the gaps.

[0014] Furthermore, in the method of the present invention, the solvent of the allicin solution is seawater, and the pH of the allicin solution is 6-8.

[0015] Furthermore, in the method of the present invention, the attachment base is any one or more combinations of corrugated plates, shells, plastic plates, and ceramic sheets on which benthic diatoms grow.

[0016] Furthermore, in the method of the present invention, the cleaning and temporary culture process of the collected juvenile sea urchins includes rinsing with natural seawater 2-3 times to remove residual allicin solution and impurities, and then transferring them to a breeding pond for subsequent breeding. The breeding environment includes a water temperature of 22-24°C, a salinity of 28-30‰, a dissolved oxygen content of 5.50-6.00 mg / L, and regular water changes and water quality monitoring.

[0017] The principle of allicin on the self-detachment of juvenile sea urchins is as follows: allicin is an irritant with a strong odor and chemical activity. Allicin can stimulate the sensory system of juvenile sea urchins, causing a stress response and leading to escape behavior in a short period of time. Juvenile sea urchins are very sensitive creatures, and any drastic changes in the environment (such as strong irritants in the water) may cause them to fall off, die or escape. On the one hand, allicin at an appropriate concentration has less toxicity to juvenile sea urchins, and on the other hand, it can interfere with the normal physiological activities of juvenile sea urchins by affecting the nerve and enzyme activity in the pod cells of juvenile sea urchins, thereby leading to their self-detachment.

[0018] The superoxide dismutase, malondialdehyde, glutathione peroxidase, and catalase are all closely related to the sea urchin immune system and play an important role in the body's resistance to oxidative stress and maintaining redox balance. SOD mainly removes superoxide anion free radicals in the body ( ), which plays an antioxidant and cell-protective role. MDA is a product of oxidative stress and is used as an important indicator of cell oxidative damage, reflecting the level of lipid peroxidation. GPx can effectively remove hydrogen peroxide and lipid peroxides in cells, protecting biological membranes and organelles (such as mitochondria) from oxidative damage. CAT decomposes hydrogen peroxide in cells ( ) is water and oxygen, preventing hydrogen peroxide accumulation from causing oxidative damage to cells. In this article, it is used to indicate the degree of damage of allicin to young bile.

[0019] The method for collecting young sea urchins using allicin described in the present invention has the following beneficial effects: On the one hand, the method of collecting young sea urchins using allicin described in the present invention stimulates the self-peeling of young sea urchins through the biological activity of allicin, effectively avoids the physical damage caused to young sea urchins by traditional mechanical peeling methods, and significantly improves the survival rate of young sea urchins after peeling. Using the method of the present invention, the peeling rate of young sea urchins on the attachment substrate is greater than or equal to 90%, and the survival rate of young sea urchins peeled off from the attachment substrate is greater than or equal to 88%. The content of SOD in the pod cells of young sea urchins peeled off from the attachment substrate is in the range of 6000-14000 U / mgprot, the content of MDA is in the range of 80-320nmol / mgprot, the content of GPx is in the range of 5000-10000 U / mgprot, and the enzyme content of CAT is in the range of 30-80 U / mgprot. The results show that allicin has no obvious coercive and toxic effects on young gallbladders under the above-mentioned appropriate conditions.

[0020] On the other hand, allicin has a wide range of sources, a relatively simple extraction process, and low cost. Compared with some special chemical stripping agents or biological agents, it is more suitable for large-scale sea urchin farming and production applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The morphology of the free eight-armed sea urchin of the present invention; Figure 2 It is the immature gallbladder attached to the corrugated plate under the microscope in the present invention. DETAILED DESCRIPTION

[0022] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described in detail with reference to the accompanying drawings. The described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. If the specific conditions are not specified in the specific implementation method, they shall be carried out according to the conventional conditions or the conditions provided by the manufacturer.

[0023] When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present disclosure. The terms used in the present disclosure are only for the purpose of describing specific embodiments and are not intended to limit the present disclosure. Specific implementation method one:

[0025] A method for collecting young sea urchins using allicin, comprising soaking a substrate with young urchins attached in an allicin solution, soaking for 0.5-1.5 minutes, and collecting young sea urchins that are peeled off from the substrate on their own; the peeling rate of young sea urchins on the substrate is greater than or equal to 90%, and the survival rate of young sea urchins that are peeled off from the substrate on their own is greater than or equal to 88%; The enzymes in the tube foot cells of juvenile sea urchins that were detached from the substrate spontaneously included superoxide dismutase, malondialdehyde, glutathione peroxidase and catalase.

[0026] In other embodiments, the superoxide dismutase content in the tube foot cells of the juvenile sea urchins that are peeled off from the attachment substrate is in the range of 6000-14000 U / mgprot, the malondialdehyde content is in the range of 80-320 nmol / mgprot, the glutathione peroxidase content is in the range of 5000-10000 U / mgprot, and the catalase content is in the range of 30-80 U / mgprot. Specific implementation method 2:

[0028] A method for collecting young sea urchins using allicin based on the above-mentioned specific embodiment 1, the method specifically comprises the following steps: 1) Pretreatment of juvenile sea urchins The substrate with the young sea urchins adsorbed is placed in a temporary culture pond, and the temporary culture environment includes: using natural seawater, the water temperature is 22-24 ℃, the salinity is 28-30‰, the dissolved oxygen content is 5.5-6.0 mg / L, and the temporary culture time is 12-24 hours; 2) Treatment of the substrate with juvenile sea urchins Taking out the attachment base with the young sea urchins adsorbed from the temporary culture pond, and then immersing the attachment base with the young sea urchins adsorbed in an allicin solution with a mass concentration of 1-20 mg / L of allicin, and controlling the immersion time to be 0.5-1.5 min; 3) Collecting juvenile sea urchins After the soaking is completed, the juvenile sea urchins that have peeled off by themselves are collected using a sieve, and the collected juvenile sea urchins are cleaned and temporarily cultured.

[0029] In other embodiments, the mass concentration of allicin in the allicin solution is 1-20 mg / L.

[0030] In other embodiments, the density of immature gallbladders in the allicin solution is 30-50 / mL.

[0031] In other embodiments, the site of action of the allicin is the tube feet extended from juvenile sea urchins.

[0032] In other embodiments, the solvent of the allicin solution is natural seawater, and the pH of the allicin solution is 6-8.

[0033] In other embodiments, the attachment base is any one or more of a combination of corrugated plates, shells, plastic plates, and ceramic sheets on which benthic diatoms grow.

[0034] In other embodiments, the cleaning and temporary culture process of the collected juvenile sea urchins includes rinsing with natural seawater 2-3 times to remove residual allicin solution and impurities, and then transferring them to a breeding pond for subsequent breeding. The breeding environment includes a water temperature of 22-24°C, a salinity of 28-30‰, a dissolved oxygen content of 5.5-6.0 mg / L, and regular water changes and water quality monitoring.

[0035] In other embodiments, the source of allicin is the natural ingredients in garlic - allicin precursor (Alliin) and allicinase (Alliinase). They generate allicin through enzymatic reactions when cutting or crushing garlic, thereby giving garlic a unique smell and biological activity. The method for extracting allicin is: select fresh, mildew-free garlic, peel it and then crush it mechanically to make garlic paste, use an extraction solvent to extract the garlic paste, the water temperature during the extraction process is 22-30 ° C, and after the extraction is completed, the extraction solvent is removed by filtering and reduced pressure distillation to obtain a high-purity allicin extract.

[0036] In other embodiments, the juvenile sea urchins are collected two weeks after the sea urchins develop to the eight-arm stage.

[0037] In other embodiments, the allicin solution is prepared by garlic powder.

[0038] The present invention is further described in detail below in conjunction with specific embodiments.

[0039] Embodiment 1:

[0040] The method for collecting young sea urchins by using allicin according to the present invention, Figure 1 This is the morphology of a free-ranging sea urchin; Figure 2 This is a young gall attached to a corrugated plate under a microscope.

[0041] The implementation steps of the method are as follows: 1) Pretreatment of juvenile sea urchins The attachment substrate with juvenile sea urchins adsorbed thereon is placed in a temporary culture pond, and the temporary culture environment includes: using natural seawater, the water temperature is 22-24 ℃, the salinity is 28-30‰, the dissolved oxygen content is 5.5-6.0 mg / L, and the temporary culture time is 12-24 h; in this embodiment 1, the attachment substrate is a corrugated board with benthic diatoms growing thereon.

[0042] 2) Treatment of the substrate with juvenile sea urchins The substrate with the young sea urchins adsorbed was taken out from the temporary holding pond, and then the substrate with the young sea urchins adsorbed was immersed in a pool of 1 mg / L allicin solution; In this embodiment 1, the solvent of the allicin solution is natural seawater, and the pH of the allicin solution is 7; the attachment base in the step 2) is a corrugated board on which the juvenile sea urchins have eaten the benthic diatoms, and the juvenile sea urchins on the corrugated board are used as samples. The corrugated board is placed horizontally and completely immersed in the allicin solution, and the corrugated board is shaken slowly for 1 min.

[0043] 3) Collecting juvenile sea urchins The sieve net collects the juvenile sea urchins that have been peeled off by themselves, and the collected juvenile sea urchins are cleaned and temporarily cultured.

[0044] In this embodiment 1, the cleaning and temporary culture process of the collected juvenile sea urchins includes rinsing with natural seawater 2-3 times to remove residual allicin solution and impurities, and then transferring them to a culture pond for subsequent culture. The culture environment includes a water temperature of 22-24 ° C, a salinity of 28-30‰, a dissolved oxygen content of 5.50-6.00 mg / L, and regular water changes and water quality monitoring.

[0045] Results Statistics: The concentration of superoxide dismutase (SOD) was detected by the A001-3-2 kit of Nanjing Jiancheng Company; The concentration of the malondialdehyde was detected by the A003-1-2 test kit of Nanjing Jiancheng Company; The concentration of the glutathione peroxidase was detected by the H545-1-1 kit produced by Nanjing Jiancheng Company; The concentration of the catalase was detected by the A007-1-1 kit produced by Nanjing Jiancheng Company; The specific detection steps were carried out strictly according to the kit instructions.

[0046] After testing, the enzyme content of SOD in the tube foot cells of the juvenile sea urchins that were peeled off from the attachment substrate was 8932U / mgprot, the content of MDA was 246nmol / mgprot, the enzyme content of GPx was 7231U / mgprot, and the enzyme content of CAT was 63U / mgprot.

[0047] Embodiment 2:

[0048] The only difference between Example 2 and Example 1 is that the mass concentration of allicin in the allicin solution is 5 mg / L; the test results are shown in Table 1.

[0049] Embodiment 3:

[0050] The only difference between Example 3 and Example 1 is that the mass concentration of allicin in the allicin solution is 15 mg / L; the test results are shown in Table 1.

[0051] Embodiment 4:

[0052] The only difference between Example 4 and Example 1 is that the mass concentration of allicin in the allicin solution is 20 mg / L; the test results are shown in Table 1.

[0053] Embodiment 5:

[0054] The difference between Example 5 and Example 1 is that the amount of the allicin solution added is 50 pieces / mL; the test results are shown in Table 1.

[0055] Embodiment 6:

[0056] The difference between Example 6 and Example 1 is that the amount of the allicin solution added is 30 pieces / mL; the test results are shown in Table 1.

[0057] Embodiment 7:

[0058] The only difference between Example 7 and Example 1 is that the action time of the allicin solution is 0.5 min; the test results are shown in Table 1.

[0059] Embodiment 8:

[0060] The only difference between Example 8 and Example 1 is that the action time of the allicin solution is 1.5 min; the test results are shown in Table 1.

[0061] Comparative Example 1: The difference between this comparative example 1 and example 1 is that the young sea urchins are collected mechanically using a soft brush, and no allicin solution is added during the whole process.

[0062] After testing, the survival rate of juvenile sea urchins that were peeled off from the attachment substrate by themselves dropped to 72%, and the natural peeling rate of juvenile gallbladders was only 6%.

[0063] Comparative Example 2: The difference between Comparative Example 2 and Example 1 is that the mass concentration of allicin in the allicin solution is 0.5 mg / L; the test results are shown in Table 1.

[0064] Comparative Example 3: The only difference between Comparative Example 3 and Example 1 is that the mass concentration of allicin in the allicin solution is 30 mg / L; the test results are shown in Table 1.

[0065] Comparative Example 4: The difference between Comparative Example 4 and Example 1 is that the amount of the allicin solution added is 55 g / mL; the test results are shown in Table 1.

[0066] Comparative Example 5: The difference between Comparative Example 5 and Example 1 is that the amount of the allicin solution added is 20 pieces / mL; the test results are shown in Table 1.

[0067] Comparative Example 6: The difference between Comparative Example 6 and Example 1 is that the action time of the allicin solution is 0.3 min; the test results are shown in Table 1.

[0068] Comparative Example 7: The difference between Comparative Example 7 and Example 1 is that the action time of the allicin solution is 3 min; the test results are shown in Table 1.

[0069] Table 1 Data of juvenile sea urchins that were peeled off from the substrate in Examples 1-8 and Comparative Examples 1-7 Allicin concentration mg / L Garlicin dosage / ml Garlicin treatment time min Superoxide dismutase U / mgprot Malondialdehyde content nmol / mgprot Glutathione peroxidase U / mgprot Catalase U / mgprot Survival rate% Peeling rate% Thorn swing amplitude and thorn state Example 1 1 40 1 8932 246 7232 63 94 97 normal Example 2 5 40 1 8359 199 8151 61 92 98 normal Example 3 15 40 1 7455 2366 8272 59 92 96 normal Example 4 20 40 1 6902 2077 7663 58 91 99 normal Example 5 1 50 1 8504 2401 9166 62 90 93 normal Example 6 1 30 1 7287 1873 8288 64 88 90 normal Example 7 1 40 0.5 9304 1794 7780 62 91 90 normal Example 8 1 40 1.5 6773 2185 7909 63 89 90 normal Comparative Example 1 0 40 1 8843 2373 7373 66 72 6 Damaged Comparative Example 2 0.5 40 1 8633 2562 8266 54 93 16 normal Comparative Example 3 30 40 1 15721 3909 11307 129 53 95 normal Comparative Example 4 1 55 1 7590 2600 8574 63 72 43 normal Comparative Example 5 1 20 1 9976 2784 8672 67 53 51 Low Comparative Example 6 1 40 0.3 9040 2399 9489 66 74 32 normal Comparative Example 7 1 40 3 13303 3843 10940 114 49 55 Low In summary, in Examples 1-8 of the present invention, the collected juvenile sea urchins were observed and found to be complete in appearance, normal in morphology, purple-black in color, with obvious tube foot activity, and good feeding ability. In the comparative examples, either the survival rate was too low or the peeling rate was too low. The results of the four immune indicators show that allicin has no obvious toxicological effects on juvenile galls within the range of added amount, and can be used as an excellent choice for shedding and transferring juvenile gall seedlings during juvenile gall cultivation. In addition, the soaking time of allicin is also crucial for the physiological survival of juvenile galls. Too long a time will cause the activity of sea urchins to weaken in a short period of time and even affect the long-term survival. Too short a soaking time cannot achieve the purpose of collecting the shed juvenile galls.

[0070] The present invention is described by the above specific embodiments, and those skilled in the art should understand that various changes and equivalent substitutions can be made to the present invention without departing from the scope of the present invention. The parts not described in detail in the present specification are well-known technologies to those skilled in the art. In addition, various modifications can be made to the present invention for specific situations or specific circumstances without departing from the scope of this new use. Therefore, the present invention is not limited to the disclosed specific embodiments, but should include all implementation methods falling within the scope of protection of the present invention.

Claims

1. A method for collecting young sea urchins using allicin, characterized in that: The method comprises soaking the attachment substrate with the juvenile sea urchins attached in a garlic solution for 0.5-1.5 minutes, and collecting the juvenile sea urchins peeled off from the attachment substrate on their own; the peeling rate of the juvenile sea urchins on the attachment substrate is greater than or equal to 90%, and the survival rate of the juvenile sea urchins peeled off from the attachment substrate on their own is greater than or equal to 88%; The enzymes in the tube foot cells of juvenile sea urchins that were detached from the substrate spontaneously included superoxide dismutase, malondialdehyde, glutathione peroxidase and catalase.

2. The method for collecting young sea urchins by utilizing allicin according to claim 1, wherein: The superoxide dismutase content in the tube foot cells of juvenile sea urchins that were peeled off from the substrate was in the range of 6000-14000 U / mgprot, the malondialdehyde content was in the range of 80-320 nmol / mgprot, the glutathione peroxidase content was in the range of 5000-10000 U / mgprot, and the catalase content was in the range of 30-80 U / mgprot.

3. The method for collecting young sea urchins using allicin according to any one of claims 1 and 2, characterized in that: The mass concentration of allicin in the allicin solution is 1-20 mg / L.

4. The method for collecting young sea urchins by utilizing allicin according to claim 3, wherein: The action site of the allicin is the tube feet extended from the young sea urchin.

5. The method for collecting young sea urchins by utilizing allicin according to claim 4, wherein: The density of immature gallbladders in the allicin solution is 30-50 gallbladders / mL.

6. The method for collecting young sea urchins using allicin according to claim 5, wherein: The method specifically comprises the following steps: 1) Pretreatment of juvenile sea urchins The substrate with the young sea urchins adsorbed is placed in a temporary culture pond, and the temporary culture environment includes: using natural seawater, the water temperature is 22-24 ℃, the salinity is 28-30‰, the dissolved oxygen content is 5.5-6.0 mg / L, and the temporary culture time is 12-24 hours; 2) Treatment of the substrate with juvenile sea urchins Taking out the attachment base with the young sea urchins adsorbed from the temporary culture pond, and then immersing the attachment base with the young sea urchins adsorbed in an allicin solution with a mass concentration of 1-20 mg / L of allicin, and controlling the immersion time to be 0.5-1.5 min; 3) Collecting juvenile sea urchins After the soaking is completed, the juvenile sea urchins that have peeled off by themselves are collected using a sieve, and the collected juvenile sea urchins are cleaned and temporarily raised.

7. The method for collecting young sea urchins using allicin according to claim 6, wherein: The solvent of the allicin solution is natural seawater, and the pH of the allicin solution is 6-8.

8. The method for collecting young sea urchins using allicin according to claim 6, wherein: The attachment base is any one or more combinations of corrugated plates, shells, plastic plates, and ceramic sheets on which benthic diatoms grow.

9. The method for collecting young sea urchins using allicin according to claim 6, wherein: The cleaning and temporary culture process of the collected juvenile sea urchins includes rinsing with natural seawater for 2-3 times to remove residual allicin solution and impurities, and then transferring them to a culture pond for subsequent culture. The culture environment includes a water temperature of 22-24°C, a salinity of 28-30‰, a dissolved oxygen content of 5.5-6.0 mg / L, and regular water changes and water quality monitoring.

Images