A compound pesticide containing validamycin and its application

Abstract

The present invention relates to the field of compound pesticides, and particularly relates to a compound pesticide containing jinggangmycin and its application. The compound pesticide containing jinggangmycin is composed of the following components by mass percentage: active ingredient 10-70%, antioxidant 1-3%, pH regulator 1-2%, and the balance is carrier and / or adjuvant; the active ingredient includes active ingredient A: jinggangmycin and active ingredient B: danshensu, D-limonene and xanthan gum, and the mass ratio of active ingredient A to active ingredient B is 1:(0.4-5). The pesticide of the present invention is compounded by active ingredient A and active ingredient B, which can significantly improve the control effect, has a good effect in controlling rice diseases, and at the same time reduces the dosage and cost of the medicine.

Description

Technical Field

[0001] The present invention relates to the technical field of compound pesticides, and particularly relates to a compound pesticide containing jinggangmycin. The present invention also relates to the application of this fungicide in the prevention and control of sheath blight, rice blast and bacterial blight of rice. Background Art

[0002] Rice is one of the most important food crops in the world, but it is often threatened by various diseases during its growth process. Rice diseases are the main factors affecting rice yield and quality. There are more than a hundred kinds of rice diseases, but the more common ones are mainly rice blast, sheath blight, leaf blight and nematode diseases. In recent years, the occurrence range of rice diseases has been continuously expanding. Despite the increasing annual investment in prevention and control, the loss of paddy rice caused by rice diseases in China is still relatively large every year.

[0003] At present, the commonly used pesticides for rice control are jinggangmycin, gibberellin, carbendazim, etc. Among them, jinggangmycin is an antibiotic produced by actinomycetes, which has strong systemic absorption, is easily absorbed by the mycelial cells and rapidly conducts inside them, interfering with and inhibiting the growth and development of the mycelial cells. It has a significant control effect on rice sheath blight, but the drug effect time is short and it needs to be used frequently, increasing the medicinal cost and also increasing the drug resistance. Summary of the Invention

[0004] In view of the above technical problems, the first object of the present invention is to provide a compound pesticide containing jinggangmycin, which is compounded from active ingredient A jinggangmycin and active ingredient B: danshensu, D-limonene and xanthan gum, can significantly improve the control effect, has a good effect in the prevention and control of rice diseases, and at the same time reduces the dosage and cost of the drug.

[0005] The second object of the present invention is to provide the application of the compound pesticide containing jinggangmycin in the prevention and control of rice sheath blight, rice blast and bacterial blight of rice.

[0006] The first object of the present invention is achieved by the following technical solutions:

[0007] A compound pesticide containing jinggangmycin is composed of the following components by mass percentage: 10-70% of active ingredient, 1-3% of antioxidant, 1-2% of pH regulator, and the balance is carrier and / or adjuvant; the active ingredient includes active ingredient A: jinggangmycin and active ingredient B: danshensu, D-limonene and xanthan gum, and the mass ratio of active ingredient A to active ingredient B is 1:(0.5-5); the mass ratio of danshensu, D-limonene and xanthan gum in active ingredient B is 10:5:0.05.

[0008] According to a preferred embodiment of the present invention, the mass ratio of active ingredient A to active ingredient B is 1:(0.5-2).

[0009] According to another preferred embodiment of the present invention, the mass ratio of the active ingredient A to the active ingredient B is 1:1.5.

[0010] According to a preferred embodiment of the present invention, the compound pesticide is composed of the following components by mass percentage: 35-50% of active ingredient, 3% of antioxidant, 1% of pH regulator, and the balance is carrier and / or auxiliary agent.

[0011] According to a preferred embodiment of the present invention, the pH value of the pesticide is 5.5-7.0.

[0012] According to a preferred embodiment of the present invention, the carrier includes but is not limited to one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyrate, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0013] According to a preferred embodiment of the present invention, the auxiliary agent includes but is not limited to one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzenesulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignosulfonate, carboxymethyl cellulose or polyvinyl alcohol.

[0014] According to a preferred embodiment of the present invention, the antioxidant includes but is not limited to any one of vitamin E, tert-butylhydroxytoluene, propyl gallate or sulfite.

[0015] According to a preferred embodiment of the present invention, the fungicide is a wettable powder, a suspension concentrate or a water dispersible granule.

[0016] When preparing the suspension concentrate of the present invention, the carrier or auxiliary agent that can be used may be a dispersant selected from sodium dodecylbenzenesulfonate, polycarboxylate or sodium lignosulfonate; a wetting agent selected from alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate or naphthalene sulfonate; an organosilicon defoamer; an adhesive selected from polyethylene glycol and glycerol.

[0017] When preparing the wettable powder of the present invention, the carrier or auxiliary agent that can be used may be a dispersant selected from polycarboxylate, sodium lignosulfonate or alkylnaphthalene sulfonate; a wetting agent selected from alkyl sulfonate, alkyl sulfate or naphthalene sulfonate; a carrier selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyrate, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0018] When preparing the water dispersible granule of the present invention, the carrier or adjuvant that can be used may be a dispersant selected from polycarboxylate, lignosulfonate or alkylnaphthalenesulfonate; a wetting agent selected from alkyl sulfate, polyvinyl alcohol, alkyl sulfonate or naphthalenesulfonate; a binder selected from corn starch, microcrystalline cellulose or diatomite; and a carrier selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyrate, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0019] The second object of the present invention is achieved by the following technical solution: the application of a compound pesticide containing jinggangmycin in the prevention and control of sheath blight, rice blast and bacterial blight of rice.

[0020] According to a preferred embodiment of the present invention, the period of applying the fungicide is the rice seed treatment period or the rice growth period, the number of applications is 1-2 times, and it is sprayed once every 10-15 days.

[0021] According to another preferred embodiment of the present invention, the concentration of the liquid medicine for each application is 300-4000 ppm.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] 1. In the compound pesticide containing jinggangmycin of the present invention, the pesticide is compounded from active ingredient A: jinggangmycin and active ingredient B: danshensu, D-limonene and xanthan gum, which has a synergistic effect, can significantly improve the control effect, has a good effect in the prevention and control of rice diseases, and at the same time reduces the dosage and cost of the pesticide. Jinggangmycin inhibits the growth and reproduction of pathogenic bacteria by producing antibiotics, enzymes, etc., but the drug effect time is short. Danshensu is a plant-derived fungicide that can prevent and control various diseases of fabrics, and can also enhance the adaptability of plants to the environment, improve productivity and disease resistance, but the effect is not good when used alone. D-limonene has the ability to inhibit the synthesis of biological macromolecules such as mycelial DNA, RNA and proteins, and has a synergistic effect on danshensu. Xanthan gum, as a stabilizer, also has a dispersing effect, can increase the full contact between D-limonene and danshensu, and prolong the drug effect. The combination of the two active ingredients can interact through different mechanisms, reduce drug resistance, further improve the disease resistance of crops, improve the protection effect and protection duration, and thus reduce the amount of pesticide used.

[0024] 2. In the compound pesticide containing validamycin of the present invention, an antioxidant is added to prevent or slow down the oxidative decomposition of pesticide components, thereby prolonging the service life and improving its field stability; at the same time, a pH regulator is added to control the acidity and alkalinity of the pesticide within an appropriate range, improving safety, stability and effectiveness; the auxiliaries or carriers used are all environmentally friendly substances, improving the application efficiency and reducing environmental pollution. With the cooperation of each component, the compound bactericide of the present invention has the advantages of high efficiency, low residue, long effective period and environmental friendliness.

[0025] 3. The compound pesticide containing validamycin of the present invention has significant effects in the application of preventing and controlling sheath blight, rice blast and bacterial blight of rice, and reduces the amount of pesticide used and has a long drug efficacy time, reducing the cost of pesticide use. Detailed implementation manners

[0026] The technical solutions of the present invention will be further described in detail below in conjunction with the specific implementation manners, but it does not constitute any limitation to the present invention.

[0027] In the present invention, the active ingredient A is validamycin, and validamycin is a commercially available drug; the active ingredient B is danshensu, D-limonene and xanthan gum. Danshensu is added to the xanthan gum aqueous solution according to the mass ratio of 10:5:0.05 in the formula amount, stirred evenly and then D-limonene is added, and stirring is continued until all components are mixed evenly, the insoluble impurities are filtered off, and dried at 60 °C to obtain the active ingredient B.

[0028] Example 1

[0029] Pesticide with the mass ratio of active ingredient A to active ingredient B being 1:0.5

[0030] Active ingredient A 30%, active ingredient B 15%, antioxidant 3%, pH regulator (phosphate buffer solution) 2%, sodium lignosulfonate 2%, polyethylene glycol 3%. Sodium lignosulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0031] Add each component into the mixer according to the formula amount, stir for 20 minutes, then send the mixture into the air flow crusher for ultrafine crushing, with the particle size below 3 microns, and then put it into the mixer again and stir for 30 minutes to make each component evenly distributed.

[0032] Example 2

[0033] Pesticide with the mass ratio of active ingredient A to active ingredient B being 1:0.75

[0034] Active ingredient A 20%, active ingredient B 15%, antioxidant 2%, pH regulator 1%, sodium lignosulfonate 2%, polyethylene glycol 3%. Sodium lignosulfonate 5%, naphthalene sulfonate 3%, the balance being lactic acid-glycolic acid copolymer.

[0035] The preparation method is the same as that of Example 1.

[0036] Example 3

[0037] Pesticide with a mass ratio of active ingredient A to active ingredient B of 1:1.5

[0038] Active ingredient A 20%, active ingredient B 30%, antioxidant 3%, pH regulator 2%, sodium lignosulfonate 2%, polyethylene glycol 3%. Sodium lignosulfonate 5%, naphthalene sulfonate 3%, the balance being lactic acid-glycolic acid copolymer.

[0039] The preparation method is the same as that of Example 1.

[0040] Example 4

[0041] Pesticide with a mass ratio of active ingredient A to active ingredient B of 1:2

[0042] Active ingredient A 10%, active ingredient B 20%, antioxidant 1%, pH regulator 1%, sodium lignosulfonate 2%, polyethylene glycol 3%. Sodium lignosulfonate 5%, naphthalene sulfonate 3%, the balance being lactic acid-glycolic acid copolymer.

[0043] The preparation method is the same as that of Example 1.

[0044] Example 5

[0045] Pesticide with a mass ratio of active ingredient A to active ingredient B of 1:2.5

[0046] Active ingredient A 20%, active ingredient B 50%, sodium lignosulfonate 2%, silicone defoamer 1%, polyethylene glycol 3%, the balance being water.

[0047] Put 20% of active ingredient A, 50% of active ingredient B, 2% of sodium lignosulfonate, 3% of polyethylene glycol and half of the water into a stone mill according to the formula amount and grind until the particle size is less than 3 microns, then disperse it into a dispersion tank, add the remaining water and defoamer, and stir at high speed for 30 minutes.

[0048] Example 6

[0049] Pesticide with a mass ratio of active ingredient A to active ingredient B of 1:5

[0050] Active ingredient A 10%, active ingredient B 50%, sodium lignosulfonate 2%, silicone defoamer 1%, polyethylene glycol 3%, the balance being water.

[0051] The preparation method is the same as that of Example 5.

[0052] Comparative Example 1

[0053] Pesticide without validamycin

[0054] Active ingredient B 30%, antioxidant 3%, pH regulator 2%, sodium lignosulfonate 2%, polyethylene glycol 3%, sodium lignosulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0055] Other steps are the same as those of Example 1.

[0056] Comparative Example 2

[0057] Pesticide without active ingredient B

[0058] Validamycin 20%, antioxidant 3%, pH regulator 2%, sodium lignosulfonate 2%, polyethylene glycol 3%, sodium lignosulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0059] The preparation method is the same as that of Example 1.

[0060] Comparative Example 3

[0061] Add D-limonene to the xanthan gum aqueous solution according to the mass ratio of 5:0.05 in the formula amount, stir evenly, filter out the insoluble impurities, and dry at 60°C to obtain active ingredient B.

[0062] Active ingredient B 30%, antioxidant 3%, pH regulator 2%, sodium lignosulfonate 2%, polyethylene glycol 3%, sodium lignosulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0063] Other steps are the same as those of Example 1.

[0064] Experimental Example 1 Determination of in vitro antibacterial activity of pesticides (Examples 1-6, Comparative Examples 1-2) containing different mass ratios of active ingredient A and active ingredient B against Rhizoctonia solani

[0065] Rhizoctonia solani was provided by Zhejiang University and purchased from ATCC.

[0066] Test method: Refer to NY / T 1156.2-2006 "Pesticide Bioassay Guidelines for Indoor Fungicides Part 2: Plate Method for Inhibiting the Growth of Pathogenic Fungal Hyphae".

[0067] (1) First, dissolve the technical material with dimethyl sulfoxide, and then dilute it into a single-agent mother liquor; set multiple groups of ratios, and each single-agent mother liquor and the mixed agent of the ratio are set according to a certain proportion, and 5 mass concentration gradients are set for each ratio.

[0068] (2) Mix the medicaments with different concentrations with PDA medium, pour the medium into plates to prepare culture medium plates containing different concentrations, and use a punch with a diameter of 7 mm to cut the cultured Rhizoctonia solani, the pathogen of rice sheath blight, and inoculate it onto the PDA plates containing a series of concentrations of the medicament; use the corresponding dimethyl sulfoxide as a control, and set a blank control. Each treatment is repeated 3 times, and all treatments are cultured in a constant temperature incubator at 28 °C.

[0069] (3) Record the results and calculate: When the colony diameter of the blank control grows to about 2 / 3 of the diameter of the culture dish, measure the colony diameter by the cross method and calculate the inhibition rate of mycelial growth for different treatments. Convert the mycelial growth inhibition rate into a probit value (y), convert the medicament concentration (mg / L) into a logarithm to the base 10, and use DPS software to obtain the virulence regression equation, the effective median inhibitory concentration (EC50) and the correlation coefficient of each test medicament, and calculate the co-toxicity coefficient (CTC value) of the mixture according to the method of Sun Yunpei.

[0070] Mycelial growth inhibition rate = [(control colony diameter - diameter of the agar disc) - (treatment colony diameter - diameter of the agar disc)] / (control colony diameter - diameter of the agar disc) × 100%;

[0071] Actual toxicity index (ATI) = (standard medicament EC50 ÷ test medicament EC50) × 100;

[0072] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of A in the mixture + toxicity index of agent B × percentage content of B in the mixture;

[0073] Co-toxicity coefficient (CTC) = [mixture actual toxicity index (ATI) ÷ mixture theoretical toxicity index (TTI)] × 100.

[0074] According to the classification standard of joint action: co-toxicity coefficient (CTC) ≥ 120 indicates synergistic effect; co-toxicity coefficient (CTC) ≤ 80 indicates antagonistic effect; 80 < co-toxicity coefficient (CTC) < 120 indicates additive effect. The experimental results are shown in Table 1.

[0075] Table 1 Indoor biological activities of pesticides with different mass ratios of A and B against Rhizoctonia solani

[0076]

[0077] As can be seen from Table 1, the EC 50 of active ingredient A and active ingredient B against Rhizoctonia solani of rice sheath blight are 18.72 mg / L and 39.25 mg / L respectively. When the mass ratio of active ingredient A to active ingredient B is in the range of 1:(0.5 - 5), the co-toxicity coefficients are all greater than 120, showing a synergistic effect.

[0078] Experimental Example 2 Control Effect of Pesticides with Active Ingredients A and B in Different Mass Ratios on Rice Diseases

[0079] Taking Examples 1 - 6 and Comparative Examples 1 - 2 as examples, field trials were conducted to test their control effects on sheath blight, rice blast, bacterial blight, and false smut of rice. The trials were carried out at the rice experimental base in Fuyang District, Zhejiang Province. The test variety was a hybrid rice variety. Each treatment had 4 replicates, and each replicate had an area of 4 square meters. 5 hill points were investigated along the two diagonals in each plot, and all the leaves of all rice plants at each hill point were investigated to count the occurrence of diseases.

[0080] The disease incidence was recorded by the grading method, and the grading criteria are as follows:

[0081] Grade 0: Disease - free;

[0082] Grade 1: The area of the disease spot pile accounts for less than 5% of the whole leaf area;

[0083] Grade 3: The area of the disease spot pile accounts for 6% - 10% of the whole leaf area;

[0084] Grade 5: The area of the disease spot pile accounts for 11% - 20% of the whole leaf area;

[0085] Grade 7: The area of the disease spot pile accounts for 21% - 50% of the whole leaf area;

[0086] Grade 9: The area of the disease spot pile accounts for more than 50% of the whole leaf area.

[0087] Calculation method of control effect

[0088] Diseased leaf rate (%) = Number of diseased leaves / Total number of investigated leaves × 100%

[0089] Disease index = ∑(Number of diseased leaves at each level × Relative level value) / (Total number of investigated leaves × 9) × 100

[0090] Control effect (%) = [1 - (Disease index before treatment in the blank control area × Disease index after treatment in the treatment area) / (Disease index after treatment in the blank control area × Disease index before treatment in the treatment area)] × 100%.

[0091] Pesticides were applied twice during the heading stage of rice, with a 10 - day interval between each application. The control effect 7 days after the second application was counted. After the completion of the test, the control effects of sheath blight, rice blast, and bacterial blight of rice were mainly counted to calculate the drug efficacy. At the same time, the control effect of false smut of rice was counted. For false smut of rice, one more count was added, and the counting time was 20 days after the second application. The control effect of false smut of rice counted this time was used as the final control effect data of false smut of rice. The control effect data of false smut of rice in the following table are the statistical data 20 days after the second application. The results are shown in Table 2.

[0092] Table 2 Statistical Table of Field Drug Efficacy Test Results

[0093]

[0094] As can be seen from Table 2, the pesticides prepared with the active ingredient A and the active ingredient B in a mass ratio of 1:(0.5 - 5) have significantly higher control effects on various common diseases of rice than the single agents of the active ingredient A jinggangmycin and the active ingredient B. There is a synergistic effect between the two in controlling rice diseases; the effect of using the active ingredient B alone is close to that of jinggangmycin. When danshensu is not contained in the active ingredient B, its control effect is greatly reduced, indicating that the compounding of danshensu with D-limonene and xanthan gum has a synergistic effect on the active ingredient B and is also a key factor in the interaction between the active ingredient A and the active ingredient B. It can also be seen from the above table that the compound pesticides of the present invention show excellent control effects on the four diseases of rice, that is, only by using the compound pesticides of the present invention can rice sheath blight, rice blast and bacterial blight be controlled simultaneously, thus achieving the control effect of "one drug for multiple prevention", greatly reducing the usage amount of chemical pesticides, reducing the cost, and at the same time being able to reduce the drug resistance to antibiotic pesticides, greatly improving the control efficiency of rice, and is very worthy of promotion.

[0095] The above embodiments are the preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A compound pesticide containing Jinggangmycin, characterized in that: The invention is composed of the following components by mass percentage: 10-70% of active components, 1-3% of antioxidants, 1-2% of pH regulators, and the remainder being carriers and / or adjuvants; the active components include active component A: Jinggangmycin and active component B: danshensu, D-limonene and xanthan gum, and the mass ratio of the active component A to the active component B is 1:(0.5~5); the mass ratio of danshensu, D-limonene and xanthan gum in the active component B is 10:5:0.

05.

2. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The mass ratio of the active component A to the active component B is 1:(0.5-2).

3. A compound pesticide containing Jinggangmycin according to claim 1 or 2, characterized in that: The mass ratio of the active component A to the active component B is 1:1.

5.

4. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The composition is composed of the following components by mass percentage: 35-50% of active component, 3% of antioxidant, 1% of pH adjuster, and the balance is carrier and / or auxiliary agent.

5. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The auxiliary agent includes, but is not limited to, one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.

6. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The carrier includes, but is not limited to, one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or polyalkyl cyanoacrylate.

7. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The antioxidant includes but is not limited to any one of vitamin E, tert-butylhydroxytoluene, propyl gallate or sulfite.

8. The compound pesticide containing Jinggangmycin according to claim 1, characterized in that: The pH value of the pesticide is 5.5-7.

0.

9. Use of the compound pesticide containing Jinggangmycin according to claim 1 in preventing and controlling rice sheath blight, rice blast and bacterial leaf blight.

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