Simulation in-vivo environment device for autograft tooth extracorporeal root canal therapy and simulation in-vivo environment preservation method
By designing a simulant in vivo environmental device, using clamping components to fix the neck of the ex vivo and moisturizing components to keep the roots moist, the problems of tooth fixation and periodontal membrane cell activity maintenance during external root canal treatment in the prior art are solved, and the success rate of tooth transplantation is significantly improved.
Patent Information
- Application Number
- CN202411984999.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-08
AI Technical Summary
The prior art is difficult to effectively fix the ex vivo teeth during the treatment of autologous transplanted teeth in vitro root canal, resulting in decreased periodontal membrane cell activity, root surface contamination and transplant failure.
A simile in vivo environmental device is designed, including a clamping assembly and a moisturizing assembly. The clamping assembly fixes the neck of the ex vivo dental through a clamping module and a fastening module, and the moisturizing assembly keeps the roots moist through a microporous atomizing sheet and a water-absorbing cotton swab.
Effective fixation of ex vivo teeth and maintenance of periodontal membrane cells during the in vitro root canal treatment are achieved, contamination of the root surface of the teeth is avoided, and the success rate of tooth transplantation is improved.
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Figure CN119770203A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of clinical application of oral medicine, relates to medical device technology, and in particular to a simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth and a simulated in vivo environment preservation method. Background Art
[0002] Root canal therapy is the most commonly used and effective treatment for pulp disease and periodontal disease. The principle of root canal therapy is to remove most of the infected substances in the root canal by mechanical and chemical methods, and to prevent the occurrence of periodontal lesions or promote the healing of existing periodontal lesions by filling the root canal and sealing the crown.
[0003] The conventional operation methods of autologous transplantation in vitro root canal treatment can be divided into the following two types:
[0004] 1) Hold the root of the excised tooth with bare hands, wrap the root or crown of the excised tooth with gauze soaked in saline to prevent the periodontal membrane from drying and contamination, and quickly perform root canal treatment;
[0005] 2) Hold the crown of the excised tooth with bare hands, spray the root surface with liquid or soak it in solution to ensure that the root surface is moistened to preserve the activity of periodontal ligament cells as much as possible, and perform root canal treatment quickly.
[0006] Using wet gauze to wrap the root surface will cause physical damage to the periodontal membrane of the root surface, and the immersion solution will be contaminated by the chemical irrigants used in root canal treatment; ex vivo teeth are difficult to hold and are prone to accidental fall off during in vitro treatment operations, and the periodontal membrane of the root surface is easily dehydrated, damaged and contaminated, which can lead to poor prognoses such as apical inflammation, internal and external root resorption, and even root shedding after tooth transplantation. Summary of the invention
[0007] In order to solve the deficiencies of the prior art, the purpose of the present invention is to better fix ex vivo teeth during in vitro root canal treatment, while maintaining the activity of periodontal ligament cells and avoiding contamination of the root surface to improve the success rate of tooth transplantation.
[0008] The present invention provides a simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth, the simulated in vivo environment device comprising: a clamping component, a moisturizing component and a base;
[0009] The clamping component and the moisturizing component are both installed on the base, the clamping component fixes the neck of the ex-vivo tooth, and the moisturizing component moisturizes the root of the ex-vivo tooth.
[0010] Preferably, the clamping assembly comprises: a clamping module and a fastening module;
[0011] The clamping module comprises: a first clamping block and a second clamping block;
[0012] The fastening module comprises: a pressing block and a bolt;
[0013] The base is provided with a slot and a slide groove, the front of the slot is connected with the slide groove, and a nut is fixedly installed at the rear;
[0014] The first clamping block and the second clamping block clamp the neck of the excised tooth and then insert into the slot; the clamping block is slidably connected to the slide slot, and the bolt passes through the clamping block and is threadedly connected to the nut; the clamping block presses and fixes the clamping module in the slot by tightening the bolt.
[0015] Preferably, the clamping function position of the first clamping block is provided with: a first avoidance notch extending in the front-to-back direction, a first accommodating groove extending in the left-to-right direction, and a first plug hole extending in the vertical direction, and a first glue stick is inserted in the first plug hole; the clamping function position of the second clamping block is provided with: a second avoidance notch, a second accommodating groove, a second plug hole, and a second glue stick correspondingly;
[0016] The clamping module further includes: a first gasket;
[0017] The first gasket is sleeved on the neck of the explanted tooth, and the first avoidance gap and the second avoidance gap avoid the explanted tooth; the first gasket is inserted into the first receiving groove and the second receiving groove, and is clamped and fixed by the first glue stick and the second glue stick.
[0018] Preferably, a first jacket is installed on the outer side of the first clamping block, and a second jacket is installed on the outer side of the second clamping block.
[0019] Preferably, an isolation plate is disposed behind the clamping module, and the isolation plate is provided with an isolation hole for the ex-vivo tooth to pass through.
[0020] Preferably, a second gasket is arranged behind the pressing block, and the pressing block contacts the clamping module through the second gasket.
[0021] Preferably, the moisturizing component comprises: a water supply tank, a drainage tank, a water-absorbing cotton swab, a microporous atomizing sheet, an atomizing driving circuit board and an isolation cover;
[0022] A hollow cavity is provided inside the base, in which a water supply trough, a drainage trough and an atomization drive circuit board are installed; one end of the absorbent cotton swab is inserted into the water supply trough, and the other end passes through the top plate of the base and is connected to the microporous atomization sheet; the microporous atomization sheet is electrically connected to the atomization drive circuit board; a drainage pipe extending to the top of the drainage trough is provided on the top plate of the base; an isolation cover is arranged above the microporous atomization sheet, the drainage pipe and the root of the excised tooth.
[0023] Preferably, the isolation cover is snap-connected to the top plate of the base.
[0024] Preferably, an isolation cavity is provided in the isolation cover, an isolation opening is provided in front of the isolation cavity, the isolation opening is fitted with the isolation plate, and the root cover of the ex vivo tooth is arranged in the isolation cavity;
[0025] The isolation chamber is provided with a mist inlet hole and a drainage hole. The mist inlet hole is connected with the microporous atomization sheet, and the drainage hole is connected with the drainage pipe. The drainage hole is arranged at the lowest point of the isolation chamber.
[0026] Preferably, the water supply trough and the drainage trough are inserted into the hollow cavity of the base through drawer openings opened on the left and right sides of the base.
[0027] Based on the above device, the present invention also proposes a method for preserving a simulated in vivo environment for in vitro root canal treatment of autologous transplanted teeth that is not intended for the treatment and diagnosis of a disease, comprising the following steps:
[0028] Step 1: Put the first gasket on the neck of the excised tooth;
[0029] Step 2: Use a glue stick to insert into the hole of the clamping block; install the first clamping block and the second clamping block on the left and right sides of the first gasket on the cervical part of the excised tooth; install the clamping sleeve on the outside of the clamping block and combine it with the isolation plate assembly to form a clamping assembly;
[0030] Step 3: Insert the clamping assembly into the slot above the base; insert the clamping block into the slide slot by placing the second washer, and then tighten the bolts to fix the clamping assembly on the base;
[0031] Step 4: Insert the absorbent cotton swab into the corresponding absorbent hole on the base; insert the isolation cover into the corresponding groove on the base; insert the upper structure of the microporous atomizer sheet into the corresponding hole of the isolation chamber; connect the lower structure of the microporous atomizer sheet to the atomization drive circuit board;
[0032] Step 5: Inject the nutrient solution into the water supply tank; insert the water supply tank and the drainage tank into the base respectively;
[0033] Step 6: Start the atomization drive circuit board and start atomization;
[0034] Step 7: The dirt and sewage generated in the isolation room flows from the drain to the drain tank.
[0035] Step 8: Turn off the atomization drive circuit board, remove the isolation cover, loosen the bolts, remove the entire clamping assembly and lay it flat (root apex facing up, crown facing down), gradually remove the isolation plate, the first gasket, the first sleeve, the first clamping block, the second clamping block, and the second sleeve, and implant the ex vivo tooth into the alveolar socket of the recipient area.
[0036] Preferably, the microporous atomization sheet has a driving voltage of DC3V-12V, atomization volume of 25-660ml / H, a resonance frequency of 103113KHZ+ / -5, and a rated power of 1.5W.
[0037] Compared with the prior art, the simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth proposed by the present invention has the following beneficial effects:
[0038] In the present invention, the simulated in vivo environment device clamps the neck of the ex vivo tooth and fixes it on the base through the clamping component, and moisturizes the root of the ex vivo tooth with nutrient mist through the moisturizing component; the effective fixation of the ex vivo tooth during in vitro root canal treatment is achieved, and the activity of periodontal ligament cells is maintained, the contamination of the root surface is avoided, and the success rate of tooth transplantation is improved; the clamping module sets a first gasket on the neck of the ex vivo tooth, and the first avoidance gap and the second avoidance gap avoid the ex vivo tooth, the first gasket is inserted into the first accommodating groove and the second accommodating groove, and is clamped and fixed by the first glue stick and the second glue stick, thereby achieving stable clamping of the neck of the ex vivo tooth; the moisturizing component atomizes the nutrient solution in the water supply tank through the absorbent cotton swab and the microporous atomizing sheet, and the nutrient mist enters the isolation chamber through the mist inlet hole to moisturize the root of the ex vivo tooth therein, maintains the activity of the periodontal ligament cells, and the sewage condensed by the nutrient mist flows into the drainage tank through the drainage hole and the drainage pipe, effectively avoiding the contamination of the root surface and improving the success rate of tooth transplantation. BRIEF DESCRIPTION OF THE DRAWINGS
[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.
[0040] Figure 1 1 is an exploded view of a simulated in vivo environment device in an embodiment of the present invention.
[0041] Figure 2 Detailed description of the invention FIG. 1 is an exploded view of a clamping module in an embodiment of the present invention.
[0042] Figure 3 It is a longitudinal cross-sectional view of the simulated in vivo environment device in an embodiment of the present invention.
[0043] Figure 4 It is a longitudinal cross-sectional view of another position of the simulated in vivo environment device in an embodiment of the present invention.
[0044] Figure 5 It is an axonometric diagram of the simulated in vivo environment device in an embodiment of the present invention.
[0045] Figure 6 FIG. 4 is an axonometric view of the simulated in vivo environment device according to an embodiment of the present invention from another angle.
[0046] Figure 7 Schematic diagram of the structure of the drainage groove in the embodiment of the present invention.
[0047] Figure 8 Schematic diagram of the structure of the water supply tank in the embodiment of the present invention.
[0048] Fig. 9 Schematic diagram of the structure of the compression block in the embodiment of the present invention.
[0049] Fig.10 Schematic diagram of the structure of the bolt in the embodiment of the present invention.
[0050] Fig.11 Schematic diagram of the structure of the isolation cover in an embodiment of the present invention.
[0051] Fig.12 Schematic diagram of the structure of the atomization drive circuit board in an embodiment of the present invention.
[0052] Fig.13 Schematic diagram of the structure of the base in an embodiment of the present invention.
[0053] Fig.14 Schematic diagram of the structure of the microporous atomizer in an embodiment of the present invention.
[0054] Fig.15 , 16 Schematic diagram of the structure of the clamping module in an embodiment of the present invention.
[0055] Figure 17-Figure 20 Schematic diagram of base installation in an embodiment of the present invention.
[0056] Fig.21 It is a schematic diagram of the clamping block of the present invention.
[0057] Fig. 22 It is a schematic diagram of the installation of the device of the present invention.
[0058] Fig.23 This is a schematic diagram of the installation of the compression block of the present invention.
[0059] Fig.24 This is a schematic diagram of the cooperation between the first clamping block and the second clamping block of the present invention.
[0060] Fig.25 It is a schematic diagram of the method for preserving a simulated in vivo environment for in vitro root canal treatment of an autologous transplanted tooth according to the present invention. DETAILED DESCRIPTION
[0061] The invention is further described in detail with reference to the following specific embodiments and drawings. The process, conditions, experimental methods, etc. for implementing the present invention, except for the contents specifically mentioned below, are all common knowledge and common common sense in the art and are not particularly limited by the present invention.
[0062] Figure 1-Figure 6Among them, 10-base; 11-slot; 12-slide; 13-nut; 21-first clamping block; 22-second clamping block; 23-first avoidance gap; 24-first accommodating groove; 25-first plug hole; 26-first glue stick; 27-second avoidance gap; 28-second plug hole; 29-second glue stick; 210-first gasket; 211-first jacket; 212-second jacket; 213-isolation plate; 214-isolation hole; 31-clamping block; 32-bolt; 33-second gasket; 34-drain pipe; 41-water supply trough; 42-drainage trough; 43-water-absorbing cotton swab; 44-microporous atomization sheet; 45-atomization drive circuit board; 46-isolation cover; 47-isolation chamber; 48-mist inlet hole; 49-drainage hole.
[0063] Example
[0064] like Fig.25 As shown, the method for preserving a simulated in vivo environment for in vitro root canal treatment of an autologous transplanted tooth in this embodiment comprises the following steps:
[0065] Step 1: Put the first gasket on the neck of the excised tooth;
[0066] Step 2: Use a glue stick to insert into the hole of the clamping block; install the first clamping block and the second clamping block on the left and right sides of the first gasket on the cervical part of the excised tooth; install the clamping sleeve on the outside of the clamping block and combine it with the isolation plate assembly to form a clamping assembly;
[0067] Step 3: Insert the clamping assembly into the slot above the base; insert the clamping block into the slide slot by placing the second washer, and then tighten the bolts to fix the clamping assembly on the base;
[0068] Step 4: Insert the absorbent cotton swab into the corresponding absorbent hole on the base; insert the isolation cover into the corresponding groove on the base; insert the upper structure of the microporous atomizer into the corresponding hole of the isolation chamber; connect the lower structure of the microporous atomizer to the atomization drive circuit board; the microporous atomizer drive voltage: DC3V-12V; atomization volume: 25-660ml / H; resonance frequency: 103113KHZ+ / -5; rated power: 1.5W.
[0069] Step 5: Inject the nutrient solution into the water supply tank; insert the water supply tank and the drainage tank into the base respectively;
[0070] Step 6: Start the atomization drive circuit board and start atomization;
[0071] Step 7: The dirt and sewage generated in the isolation room flows from the drain to the drain tank.
[0072] Step 8: Turn off the atomization drive circuit board, remove the isolation cover, loosen the bolts, remove the entire clamping assembly and lay it flat (root apex facing up, crown facing down), gradually remove the isolation plate, the first gasket, the first sleeve, the first clamping block, the second clamping block, and the second sleeve, and implant the ex vivo tooth into the alveolar socket of the recipient area.
[0073] like Figure 1-Figure 24 As shown, the simulated in vivo environment device includes: a clamping component, a moisturizing component and a base 10;
[0074] The clamping assembly and the moisturizing assembly are both installed on the base 10. The clamping assembly fixes the neck of the ex-vivo tooth, and the moisturizing assembly moisturizes the root of the ex-vivo tooth.
[0075] like Figure 1-Figure 24 As shown, the clamping assembly includes: a clamping module and a fastening module;
[0076] The clamping module comprises: a first clamping block 21 and a second clamping block 22;
[0077] The fastening module comprises: a pressing block 31 and a bolt 32;
[0078] The base 10 is provided with a slot 11 and a slide groove 12. The slot 11 is connected to the slide groove 12 in front and a nut 13 is fixedly installed in the rear.
[0079] The first clamping block 21 and the second clamping block 22 clamp the neck of the excised tooth and then insert into the slot 11; the clamping block 31 is slidably connected to the slide groove 12, and the bolt 32 passes through the clamping block 31 and is threadedly connected to the nut 13; the clamping block 31 presses and fixes the clamping module in the slot 11 by tightening the bolt 32.
[0080] like Figure 2 As shown, the first clamping block 21 is provided at the clamping function position with: a first avoidance notch 23 extending in the front-to-back direction, the notch having a width of 1 mm, a first receiving groove 24 extending in the left-right direction, and a first plug hole 25 extending in the vertical direction, a first glue stick 26 being inserted in the first plug hole 25; the second clamping block 22 is provided at the clamping function position with: a second avoidance notch 27 having a width of 1 mm, a second receiving groove, a second plug hole 28, and a second glue stick 29;
[0081] After the glue sticks are introduced and the excised tooth is not installed, the relative gap between the two glue sticks in the clamping module is 1 mm, and the deepest distance between them and the avoidance gap is 9.5 mm.
[0082] The clamping module further includes: a first gasket 210;
[0083] The first gasket 210 is sleeved on the neck of the ex-vivo tooth, and the first avoidance gap 23 and the second avoidance gap 27 avoid the ex-vivo tooth; the first gasket 210 is inserted into the first accommodating groove 24 and the second accommodating groove, and is clamped and fixed by the first glue stick 26 and the second glue stick 29, so that the clamping module clamps and fixes the neck of the ex-vivo tooth.
[0084] like Figure 2 As shown, a first clamping sleeve 211 is installed on the outer side of the first clamping block 21 , and a second clamping sleeve 212 is installed on the outer side of the second clamping block 22 .
[0085] like Figure 2 As shown, an isolation plate 213 is disposed at the rear of the clamping module, and the isolation plate 213 is provided with an isolation hole 214 for the ex-vivo tooth to pass through.
[0086] like Figure 1 As shown, a second gasket 33 is disposed behind the pressing block 31 , and the pressing block 31 contacts the clamping module through the second gasket 33 .
[0087] like Figure 1 , Figure 3 , Figure 4 As shown, the moisturizing component includes: a water supply tank 41, a drainage tank 42, a water-absorbing cotton swab 43, a microporous atomizing sheet 44, an atomizing driving circuit board 45 and an isolation cover 46;
[0088] The base 10 is provided with a hollow cavity inside, in which a water supply groove 41, a drainage groove 42 and an atomization drive circuit board 45 are installed; one end of a water-absorbing cotton swab 43 is inserted into the water supply groove 41, and the other end passes through the top plate of the base 10 and is connected to a microporous atomization sheet 44; the microporous atomization sheet 44 is electrically connected to the atomization drive circuit board 45; the top plate of the base 10 is provided with a drainage pipe 34 extending to above the notch of the drainage groove 42; an isolation cover 46 is provided above the microporous atomization sheet 44, the drainage pipe 34 and the root of the excised tooth.
[0089] like Figure 1 , Figure 3-Figure 6 As shown, the isolation cover 46 is snap-connected to the top plate of the base 10 for easy assembly and disassembly.
[0090] like Figure 3 , Figure 4 As shown, an isolation chamber 47 is provided in the isolation cover 46, an isolation opening is provided in front of the isolation chamber 47, and the isolation opening is fitted with the isolation plate 213, and the root cover of the ex vivo tooth is arranged in the isolation chamber 47;
[0091] The isolation chamber 47 is provided with a mist inlet hole 48 and a drainage hole 49 . The mist inlet hole 48 is connected to the microporous atomization sheet 44 , and the drainage hole 49 is connected to the drainage pipe 34 . The drainage hole 49 is provided at the lowest point of the isolation chamber 47 .
[0092] like Figure 1 As shown, the water supply trough 41 and the drainage trough 42 are inserted into the hollow cavity of the base 10 through drawer openings opened on the left and right sides of the base 10, so as to facilitate the loading and unloading of the water supply trough 41 and the drainage trough 42.
[0093] The materials and dimensions of the various components in this embodiment are shown in the following table:
[0094]
[0095] The protection content of the present invention is not limited to the above embodiments. Without departing from the spirit and scope of the inventive concept, changes and advantages that can be thought of by those skilled in the art are included in the present invention and are protected by the attached claims.
Claims
1. A simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth, characterized in that: include: A clamping component, a moisturizing component and a base (10); wherein, The clamping component and the moisturizing component are both installed on the base (10); the clamping component fixes the neck of the ex-vivo tooth, and the moisturizing component moisturizes the root of the ex-vivo tooth.
2. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 1, characterized in that: The clamping assembly includes: a clamping module and a fastening module; wherein, The clamping module comprises: a first clamping block (21) and a second clamping block (22); The fastening module comprises: a pressing block (31) and a bolt (32); The base (10) is provided with a slot (11) and a slide groove (12); the slot (11) is connected to the slide groove (12) at the front, and a nut (13) is fixedly installed at the rear; The first clamping block (21) and the second clamping block (22) clamp the neck of the excised tooth and then are inserted into the slot (11) to limit the positions on both sides; the clamping block (31) is slidably connected to the slide groove (12), and the bolt (32) passes through the clamping block (31) and is threadedly connected to the nut (13); the clamping block (31) presses and fixes the clamping module in the slot (11) by tightening the bolt (32).
3. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 2, characterized in that: The first clamping block (21) is provided with a first avoidance notch (23) extending in the front-to-back direction, a first receiving groove (24) extending in the left-to-right direction, and a first plug hole (25) extending in the vertical direction at the clamping function position, wherein a first glue stick (26) is inserted into the first plug hole (25); the second clamping block (22) is provided with a corresponding second avoidance notch (27), a second receiving groove, a second plug hole (28), and a second glue stick (29) at the clamping function position; The clamping module further comprises: a first gasket (210); The first gasket (210) is sleeved on the neck of the ex-vivo tooth, and the first avoidance notch (23) and the second avoidance notch (27) avoid the ex-vivo tooth; the first gasket (210) is inserted into the first receiving groove (24) and the second receiving groove, and is clamped and fixed by the first glue stick (26) and the second glue stick (29).
4. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 2, characterized in that: A first clamping sleeve (211) is installed on the outer side of the first clamping block (21), and a second clamping sleeve (212) is installed on the outer side of the second clamping block (22); The first clamping sleeve (211) is combined with the first clamping block (21), and the second clamping sleeve (212) is combined with the second clamping block (22), and then the whole is inserted into the slot 11.
5. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 2, characterized in that: An isolation plate (213) is arranged at the rear of the clamping module, and the isolation plate (213) is provided with an isolation hole (214) for the ex-vivo tooth to pass through.
6. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 2, characterized in that: A second gasket (33) is arranged behind the pressing block (31), and the pressing block (31) is in contact with the clamping module through the second gasket (33).
7. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 1, characterized in that: The moisturizing component comprises: a water supply tank (41), a drainage tank (42), a water-absorbing cotton swab (43), a microporous atomizing sheet (44), an atomizing driving circuit board (45) and an isolation cover (46); wherein: The base (10) is provided with a hollow cavity inside, and a water supply groove (41), a drainage groove (42) and an atomization drive circuit board (45) are installed in the hollow cavity; one end of the absorbent cotton swab (43) is inserted into the water supply groove (41), and the other end passes through the top plate of the base (10) and is connected to the microporous atomization sheet (44); the microporous atomization sheet (44) is electrically connected to the atomization drive circuit board (45); the top plate of the base (10) is provided with a drainage pipe (34) extending to the top of the notch of the drainage groove (42); the isolation cover (46) is arranged above the microporous atomization sheet (44), the drainage pipe (34) and the root of the excised tooth.
8. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 7, characterized in that: The isolation cover (46) is snap-connected to the top plate of the base (10); or, the isolation cover (46) is magnetically connected to the base (10).
9. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth as claimed in claim 7, characterized in that: An isolation chamber (47) is provided in the isolation cover (46), an isolation opening is provided in front of the isolation chamber (47), the isolation opening is fitted with the isolation plate (213), and the root cover of the ex vivo tooth is arranged in the isolation chamber (47); The isolation chamber (47) is provided with a mist inlet hole (48) and a drainage hole (49); the mist inlet hole (48) is connected to the microporous atomization sheet (44); the drainage hole (49) is connected to the drainage pipe (34); and the drainage hole (49) is provided at the lowest point of the isolation chamber (47).
10. The simulated in vivo environment device for in vitro root canal treatment of autologous transplanted teeth according to claim 7, characterized in that: The water supply trough (41) and the drainage trough (42) are inserted into the hollow cavity of the base (10) through drawer openings opened on the left and right sides of the base (10), so that the water supply trough (41) and the drainage trough (42) are easy to load and unload.
11. A method for preserving a simulated in vivo environment for in vitro root canal treatment of an autologous tooth transplant, characterized in that: The method adopts the simulated in vivo environment device as described in any one of claims 1 to 10; the method comprises the following steps: Step 1: Put the first gasket (210) on the neck of the excised tooth; Step 2: Use a glue stick to insert into the hole of the clamping block; install the first clamping block and the second clamping block (22) on the left and right sides of the first gasket (210) on the neck of the excised tooth; install the clamping sleeve on the outside of the clamping block and combine it with the isolation plate assembly to form a clamping assembly; Step 3: insert the clamping assembly into the slot above the base; insert the clamping block (31) into the slide groove (12) by placing the second washer (33), and then tighten the bolt (32) to fix the clamping assembly on the base; Step 4: insert the absorbent cotton swab (43) into the corresponding absorbent hole on the base; insert the isolation cover (46) into the corresponding groove on the base; insert the upper structure of the microporous atomizing sheet (44) into the corresponding hole of the isolation chamber; connect the lower structure of the microporous atomizing sheet (44) to the atomizing driving circuit board (45); Step 5: injecting nutrient solution into the water supply tank (41); inserting the water supply tank (41) and the drainage tank (42) into the base respectively; Step 6: Start the atomization drive circuit board and start atomization.
12. The storage method according to claim 11, characterized in that: Further comprising the steps of: Step 7: The dirt and sewage generated in the isolation room flows from the drain to the drain tank; Step 8: Close the atomization drive circuit board, remove the isolation cover (46), loosen the bolts, remove the entire clamping assembly and lay it flat, and gradually remove the isolation plate (213), the first gasket (210), the first jacket (211), the first clamping block (21), the second clamping block (22), and the second jacket (212).
13. The storage method according to claim 11, characterized in that: The microporous atomizer has a driving voltage of DC3V-12V, atomization volume of 25-660ml / H, a resonance frequency of 103113KHZ+ / -5, and a rated power of 1.5W.