Application of natural unsaturated fatty aldehyde in prevention and control of storage pests

Abstract

The invention discloses application of natural unsaturated fatty aldehyde in prevention and control of storage pests. The natural unsaturated fatty aldehyde is trans-2, 4-heptadienal and trans-2-heptenal, and the natural unsaturated fatty aldehyde is trans-2, 4-heptadienal and trans-2-heptenal. The storage pests comprise coleoptera storage pests such as rice weevil, corn weevil, callosobruchus chinensis, coffee weevil, salopterus sajori, tribolium cavaleriei, delavay beetle, barbeque beetle, lasioderma serricorne, round bark beetle, black bark beetle and melanogaster, and lepidoptera storage pests such as corcyra cephalonica, wheat moth, Indian rice moth, diplodia rubella and mediterranean sea moth. The invention discloses that trans-2, 4-heptadienal and trans-2-heptenal can show excellent stored insect killing activity in a fumigation manner for the first time, provides a basis for developing green and environment-friendly insecticides, and has a good application prospect of replacing traditional chemical insecticides.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and particularly relates to application of natural unsaturated fatty aldehydes in preventing and controlling storage pests. Background Art

[0002] Storage pests pose a huge threat to food quality and storage safety, leading to a decline in food quality and a large amount of losses. According to a survey by the Food and Agriculture Organization of the United Nations, losses caused by stored grain pests account for about 4% to 8% of the total storage volume worldwide, and in some countries, this proportion may even be as high as 30%. Therefore, how to scientifically and effectively prevent and control stored grain pests and protect stored grain from pests has become an urgent challenge in the field of food storage safety.

[0003] At present, low-temperature grain storage technology and gas-controlled pest control technology are often used in grain storage pest control. However, physical control of low temperature and nitrogen-filled gas control (low oxygen) still faces the problem of pest occurrence, and low-temperature and low-oxygen resistant pests are difficult to completely eliminate. The application scenario of nitrogen regulation technology is mainly the grain storage environment in the south, and it has disadvantages such as long operation cycle and high requirements for environmental sealing. Chemical fumigants are effective in the application of grain storage pest control, such as sulfuryl fluoride and aluminum phosphide fumigation technology. However, relevant studies have shown that sulfuryl fluoride is a potent greenhouse gas, which has an unignorable impact on global warming. The long-term use of aluminum phosphide has also led to the enhancement of pest resistance, making it difficult to achieve the effect of efficient pest elimination. In addition, aluminum phosphide will produce a large amount of highly toxic gases during decomposition, which is harmful to human health. Although chemical control is cheap and efficient, it will endanger food security, damage the environment, and affect the health of humans and animals. Therefore, it is urgent to develop new efficient and environmentally friendly grain storage pest control technologies to supplement the deficiencies of existing control technologies.

[0004] Natural unsaturated fatty aldehydes are a class of compounds commonly found in volatile essential oils of plants. They play an important role in plant defense mechanisms against pathogenic microorganisms. These compounds not only emit fragrance, but also have important value as natural flavor resources. In particular, trans-, trans-2,4-heptadienal and trans-2-heptenal, as two typical natural unsaturated fatty aldehydes synthesized by plants, have been certified as safe edible flavors and preservatives by the "Food Additives Usage Standard" (GB2760) issued by the National Health Commission of China and the US Food and Drug Administration (FDA). This study evaluated the efficacy of these two natural unsaturated fatty aldehydes in the prevention and control of grain storage pests. The results showed that trans-, trans-2,4-heptadienal and trans-2-heptenal could effectively kill grain storage pests through fumigation treatment, thus showing their potential as new insecticides. In view of their high efficiency and safety, trans-, trans-2,4-heptadienal and trans-2-heptenal are worthy of further research and development in the field of grain storage pest control. Summary of the invention

[0005] In view of this, the object of the present invention is to provide an application of natural unsaturated fatty aldehydes in preventing and controlling storage pests. The natural unsaturated fatty aldehydes include any one of trans-, trans-2,4-heptadienal and trans-2-heptenal. Natural unsaturated fatty aldehydes also have strong volatility in the natural state and can be directly used as storage pest fumigants.

[0006] Preferably, the natural unsaturated fatty aldehyde is trans,trans-2,4-heptadienal and / or trans-2-heptenal.

[0007] Preferably, the storage pests include Coleopteran storage pests such as rice weevils, corn weevils, mung bean weevils, coffee bean weevils, saw-saw grain beetles, false flour beetles, large grain beetles, grain borers, scaly skin borers, tobacco beetles, small round skin borers, black skin borers and black fungus borers, and Lepidoptera storage pests such as rice moths, wheat moths, Indian meal borers, powdery patch borers and Mediterranean stem borers.

[0008] Preferably, the natural unsaturated fatty aldehyde is used to control storage pests by fumigation.

[0009] Preferably, the concentration of the natural unsaturated fatty aldehyde used in the fumigation is 1.5-36 μL / L.

[0010] Preferably, the concentration of trans, trans-2,4-heptadienal used in the fumigation is 20 μL / L, and the concentration of trans-2-heptenal is 36 μL / L.

[0011] The natural unsaturated fatty aldehydes trans, trans-2,4-heptadienal and trans-2-heptenal of the present invention have a broad-spectrum effect of killing storage pests, and can be used to prevent and control coleopteran storage pests including rice weevils, corn weevils, mung bean weevils, coffee bean weevils, saw-toothed grain beetles, flour beetles, large grain beetles, grain borers, spot-skin beetles, tobacco beetles, small round skin beetles, black skin beetles and black fungus beetles, and lepidopteran storage pests such as rice moths, wheat moths, Indian meal borers, pink spot borers and Mediterranean borers. Natural unsaturated fatty aldehydes are widely present in plants, are green, environmentally friendly, and have strong volatility, and can kill storage pests through fumigation. The trans, trans-2,4-heptadienal and trans-2-heptenal of the present invention have excellent storage pest killing activity, can replace traditional chemical storage pest killers, and have broad application prospects in the field of pest control. DETAILED DESCRIPTION

[0012] The present invention will be described in detail below by examples, which are intended to demonstrate the effects of the present invention and cannot represent the scope of the present invention. The protection scope of the present invention is not limited to the following embodiments or examples, and any modifications and substitutions made in accordance with the present invention should be included within the scope of the present invention. In the following examples, unless otherwise specified, the experimental reagents used can be obtained through commercial channels. The natural unsaturated fatty aldehydes trans, trans-2,4-heptadienal and trans-2-heptenal used in the following examples were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0013] Example 1: Control of stored rice pests by natural unsaturated fatty aldehydes

[0014] 1) Test pests

[0015] The adult of the rice borer originates from rice grains.

[0016] 2) Fumigants

[0017] Trans, trans-2,4-heptadienal were fumigated at 1.5μL / L, 3μL / L and 6μL / L respectively, and trans-2-heptenal were fumigated at 6μL / L, 12μL / L and 18μL / L respectively. Note: 6μL / L means that 6μL of the stock solution of trans, trans-2,4-heptadienal or trans-2-heptenal is used per cubic decimeter of space volume, and the rest are similar.

[0018] 3) Fumigation test

[0019] About 50 adults of rice borers were placed in a 1L cube acrylic box (i.e., a space volume of 1 cubic decimeter), and a concave slide was placed in the box. Samples were added according to the concentration of the fumigant described in 2) as independent treatment groups, with an equal amount of ddH2O as the control, and three replicates were performed for each treatment. The cube acrylic box was covered with a lid and sealed with a PARAFILM film. Then it was placed in a 28°C incubator for 24 hours, and the corrected mortality rate was calculated. The corrected mortality rate calculation formula is shown below:

[0020] Adjusted mortality rate = (mortality rate of treatment group - mortality rate of control group) / (1 - mortality rate of control group) × 100%.

[0021] 4) Result analysis

[0022] The 24h fumigation activity of different concentrations of trans, trans-2,4-heptadienal and trans-2-heptenal against adults of P. pygmaea is shown in Table 1. The corrected mortality rate of 6 μL / L trans, trans-2,4-heptadienal or 18 μL / L trans-2-heptenal against adults of P. pygmaea is 100%, indicating that the natural unsaturated fatty aldehydes described in the present invention have excellent fumigation activity against adults of P. pygmaea at low doses.

[0023] Table 1 Fumigation activity of natural unsaturated fatty aldehydes against adults of Pseudostellaria spp. within 24 h

[0024]

[0025] Example 2: Natural unsaturated fatty aldehydes to control the green bean pest green bean weevil in storage

[0026] 1) Test pests

[0027] The mung bean elephant comes from mung beans.

[0028] 2) Fumigants

[0029] Trans, trans-2,4-heptadienal was tested for fumigation at concentrations of 1.5 μL / L, 3 μL / L and 6 μL / L, and trans-2-heptenal was tested for fumigation at concentrations of 3 μL / L, 6 μL / L and 12 μL / L.

[0030] 3) Fumigation test

[0031] About 50 green bean weevils were placed in a 1L cube acrylic box (i.e., a space volume of 1 cubic decimeter), and a concave hole slide was placed in the box. Samples were added as independent treatment groups according to the concentration of the fumigant described in 2), and an equal amount of ddH2O was used as a control. Each treatment was repeated three times. The cube acrylic box was covered with a lid and sealed with a PARAFILM film. Then it was placed in a 22°C incubator for 24 hours, and the corrected mortality rate was calculated. The corrected mortality rate calculation formula was the same as in Example 1.

[0032] 4) Result analysis

[0033] The 24h fumigation activity of different concentrations of trans, trans-2,4-heptadienal and trans-2-heptenal against green bean weevils is shown in Table 2. The corrected mortality rate of 6 μL / L trans, trans-2,4-heptadienal or 12 μL / L trans-2-heptenal against green bean weevils was 100%, indicating that the natural unsaturated fatty aldehydes of the present invention have excellent fumigation activity against green bean weevils at a low dose.

[0034] Table 2 Fumigation activity of natural unsaturated fatty aldehydes against adults of green bean weevil within 24 hours

[0035]

[0036] Example 3: Control of stored wheat pest Pseudostellaria castanea by natural unsaturated fatty aldehydes

[0037] 1) Test pests

[0038] Castanea paniculate, originates from wheat.

[0039] 2) Fumigants

[0040] Trans, trans-2,4-heptadienal was tested for fumigation at concentrations of 1.5 μL / L, 3 μL / L and 6 μL / L, and trans-2-heptenal was tested for fumigation at concentrations of 3 μL / L, 6 μL / L and 12 μL / L.

[0041] 3) Fumigation test

[0042] About 50 adult castaneums were placed in a 1L cube acrylic box (i.e., a space volume of 1 cubic decimeter), and a concave hole slide was placed in the box. Samples were added as independent treatment groups according to the concentration of the fumigant described in 2), and an equal amount of ddH2O was used as a control, and three replicates were used for each treatment. The cube acrylic box lid was covered and sealed with a PARAFILM film. Then it was placed in a 28°C incubator for treatment for 24 hours, and the corrected mortality rate was calculated. The corrected mortality rate calculation formula was the same as in Example 1.

[0043] 4) Result analysis

[0044] The 24h fumigation activity of different concentrations of trans, trans-2,4-heptadienal and trans-2-heptenal against adults of P. castaneum is shown in Table 3. The corrected mortality rate of 6 μL / L trans, trans-2,4-heptadienal or 12 μL / L trans-2-heptenal against adults of P. castaneum was 100%, indicating that the natural unsaturated fatty aldehydes of the present invention have excellent fumigation activity against adults of P. castaneum at a low dose.

[0045] Table 3 Fumigation activity of natural unsaturated fatty aldehydes against adults of Castanea castanea within 24 h

[0046]

[0047] Example 4: Control of stored rice pest rice weevils by natural unsaturated fatty aldehydes

[0048] 1) Test pests

[0049] Rice weevils originate from rice.

[0050] 2) Fumigants

[0051] Trans, trans-2,4-heptadienal were tested by fumigation at concentrations of 3 μL / L, 6 μL / L and 12 μL / L, and trans-2-heptenal were tested by fumigation at concentrations of 12 μL / L, 24 μL / L and 36 μL / L.

[0052] 3) Fumigation test

[0053] About 50 rice weevils were placed in a 1L cube acrylic box (i.e., a space volume of 1 cubic decimeter), and a concave hole slide was placed in the box. Samples were added as independent treatment groups according to the concentration of the fumigant described in 2), and an equal amount of ddH2O was used as a control. Each treatment was repeated three times. The cube acrylic box was covered with a lid and sealed with a PARAFILM film. Then it was placed in a 26°C incubator for treatment for 24 hours, and the corrected mortality rate was calculated. The corrected mortality rate calculation formula was the same as in Example 1.

[0054] 4) Result analysis

[0055] The 24h fumigation activities of different concentrations of trans, trans-2,4-heptadienal and trans-2-heptenal against rice weevil adults are shown in Table 4. The corrected mortality rate of 12 μL / L trans, trans-2,4-heptadienal or 36 μL / L trans-2-heptenal against rice weevil adults is 100%, indicating that the natural unsaturated fatty aldehydes of the present invention have excellent fumigation activity against rice weevil adults at a low dose.

[0056] Table 4 Fumigation activity of natural unsaturated fatty aldehydes against rice weevil adults within 24 hours

[0057]

[0058] Example 5: Control of stored rice pest rice moth by natural unsaturated fatty aldehydes

[0059] 1) Test pests

[0060] Rice moth, comes from rice.

[0061] 2) Fumigants

[0062] Trans, trans-2,4-heptadienal and trans-2-heptenal were fumigated at concentrations of 5 μL / L, 10 μL / L, and 20 μL / L, respectively.

[0063] 3) Fumigation test

[0064] About 50 rice moth larvae were placed in a 1L cube acrylic box (i.e., a space volume of 1 cubic decimeter), and a concave hole slide was placed in the box. Samples were added as independent treatment groups according to the concentration of the fumigant described in 2), and an equal amount of ddH2O was used as a control, and three replicates were used for each treatment. The cube acrylic box was covered with a lid and sealed with a PARAFILM film. Then it was placed in a 26°C incubator for treatment for 24 hours, and the corrected mortality rate was calculated. The corrected mortality rate calculation formula was the same as in Example 1.

[0065] 4) Result analysis

[0066] The 24h fumigation activities of different concentrations of trans, trans-2,4-heptadienal and trans-2-heptenal on rice moth larvae are shown in Table 5. The corrected mortality of 20 μL / L trans, trans-2,4-heptadienal or trans-2-heptenal on rice moth larvae is 100%, indicating that the natural unsaturated fatty aldehydes of the present invention have excellent fumigation activity on rice moth larvae at a low dose.

[0067] Table 5 Fumigation activity of natural unsaturated fatty aldehydes against rice moth larvae within 24 hours

[0068]

[0069] In summary, the natural unsaturated fatty aldehydes trans, trans-2,4-heptadienal and trans-2-heptenal described in the present invention have excellent fumigation activity against a variety of storage pests and are worthy of further research and development in pest control.

[0070] The above embodiments and / or experimental examples describe in detail the preferred implementation modes of the present invention. However, the protection scope of the present invention is not limited to the specific details in the above implementation modes. Within the technical framework of the present invention, various direct adjustments and changes to the technical scheme of the present invention are allowed, and these adjustments and changes should be regarded as part of the protection scope of the present invention.

Claims

1. The application of natural unsaturated fatty aldehydes in preventing and controlling storage pests is characterized in that: The natural unsaturated fatty aldehyde includes trans, trans-2,4-heptadienal or trans-2-heptenal.

2. The use according to claim 1, characterized in that The natural unsaturated fatty aldehyde is trans, trans-2,4-heptadienal and / or trans-2-heptenal.

3. The use according to claim 1, characterized in that The storage pests include Coleoptera storage pests and Lepidoptera storage pests.

4. The use according to claim 1, characterized in that The coleopteran storage pests include rice weevils, corn weevils, mung bean weevils, coffee bean weevils, saw-saw grain beetles, flour beetles, large grain beetles, grain borers, spotted cuttlefish borers, tobacco beetles, small round cuttlefish borers, black cuttlefish borers and black fungus borers, and the lepidopteran storage pests include rice moths, wheat moths, Indian meal borers, pinky patch borers and Mediterranean stem borers.

5. The use according to claim 1, characterized in that The natural unsaturated fatty aldehyde is used to control storage pests by fumigation.

6. The use according to claim 4, characterized in that The concentration used for the fumigation is 0.1-1000 μL / L.

7. The use according to claim 6, characterized in that The concentration used for the fumigation is 1.5-36 μL / L.

8. The use according to claim 7, characterized in that The fumigation concentration of the trans, trans-2,4-heptadienal is 20 μL / L, and the fumigation concentration of the trans-2-heptenal is 36 μL / L.

Images