Mesenchymal stem cell exosome ovarian injection device

Abstract

The invention relates to a mesenchymal stem cell exosome ovary injection device which comprises a puncture needle, an elastic tube and an injector, a plurality of liquid outlet holes are formed in the side wall of the puncture needle, the needle tip of the puncture needle is a sharp solid needle tip, one end of the elastic tube is communicated with the puncture needle through a hollow connecting part, the other end of the elastic tube is provided with a silica gel plug, and the injector is connected with the puncture needle through a connecting part. One end, far away from the elastic tube, of the silica gel plug is provided with a guide hole which is sunken inwards, and the guide hole is used for guiding a needle head of the injector to pierce the silica gel plug. The needle tip is solid, sharp and high in rigidity, and can smoothly pierce into the ovarian tissue; a plurality of liquid outlet holes are formed in the side surface of the puncture needle, so that exosomes can be more uniformly distributed in the ovary during puncture and injection, the puncture frequency is reduced, and the physical damage to the ovary of a patient is reduced; a silica gel plug is designed at one end of the elastic tube for plugging, so that liquid backflow in the process of switching different injection liquid medicine can be prevented, and the infection risk is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and in particular relates to a mesenchymal stem cell exosome ovarian injection device. Background Art

[0002] Premature ovarian insufficiency (POI) is the most common female reproductive aging disease, which not only causes premature decline or even loss of fertility, but also threatens the patient's health. The main diagnostic criteria for POI are that women have oligomenorrhea or amenorrhea for more than 4 months before the age of 40, and the serum level of gonadotropin follicle-stimulating hormone (FSH) is greater than 25 IU / L twice tested more than 4 weeks apart. In China, about 4 million women of childbearing age suffer from POI.

[0003] At present, there are very limited clinical treatments for POI. In recent years, mesenchymal stem cells and their derived exosomes have been tried for POI treatment, and have achieved good clinical results. However, the clinical application of stem cells or exosomes to treat POI often uses laparoscopic injection, which is more traumatic to patients.

[0004] In clinical practice, vaginal ultrasound can be used for visual guidance during egg retrieval, and the egg retrieval needle enters the ovarian tissue through genital puncture, which is less traumatic. However, there are several problems when using the egg retrieval needle for exosome injection under ultrasound guidance: First, the ovaries of POI patients are relatively tight, and conventional egg retrieval needles are difficult to puncture, and the outlet is more likely to be blocked after puncture; second, it is necessary to penetrate the ovaries multiple times to achieve the effect of evenly distributing the exosomes throughout the ovaries, which causes greater damage to the patient's ovaries; third, in order to avoid the residue of exosome liquid in the injection device, different liquids need to be switched for flushing during the injection process, which poses the risk of liquid reflux and contamination. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a mesenchymal stem cell exosome injection device, which enables medical personnel to easily complete the ovarian injection of exosomes under ultrasound guidance, shorten the operation time, and reduce surgical damage to patients.

[0006] The technical solution adopted by the present invention to solve its technical problem is: to provide a mesenchymal stem cell exosome ovarian injection device, including a puncture needle, an elastic tube and a syringe, the side wall of the puncture needle is provided with a plurality of liquid outlet holes, the needle tip of the puncture needle is a sharp solid needle tip, one end of the elastic tube is connected with the puncture needle through a hollow connecting component, and the other end is provided with a silicone plug, and the end of the silicone plug away from the elastic tube is provided with an inwardly recessed guide hole, and the guide hole is used to guide the needle of the syringe to pierce the silicone plug.

[0007] Preferably, there are 6 liquid outlet holes in total, which are arranged in two rows on two opposite sides of the puncture needle.

[0008] Preferably, in the same row, the distance between two adjacent liquid outlet holes is 0.3-1.5 mm.

[0009] Preferably, the needle body of the puncture needle is 20 to 50 mm in length.

[0010] Preferably, the outer diameter of the puncture needle is 0.5-1.8 mm.

[0011] Preferably, the needle tip length of the puncture needle is 1 to 5 mm.

[0012] Preferably, the inner diameter of the liquid outlet hole is 0.1-0.3 mm.

[0013] Preferably, the needle tip of the puncture needle is a solid conical structure, and the angle between its generatrix and the height is ≤30° and >0°.

[0014] Preferably, the elastic tube has a length of 10 to 50 cm and an inner diameter of 0.6 to 1.2 mm.

[0015] Preferably, the syringe has a specification of 1 to 10 mL.

[0016] The beneficial effects are as follows: the needle tip of the present invention is a solid structure with high rigidity, which is convenient for preparing a sharper needle tip and can more smoothly penetrate into the ovarian tissue; the present invention is designed with multiple liquid outlet holes on the side of the puncture needle, which can make the exosomes more evenly distributed in the ovary during puncture and injection, reduce the number of punctures, and thus reduce the physical damage to the patient's ovary; the liquid outlet hole of the present invention is perpendicular to the needle insertion direction, which can better prevent blockage; the elastic tube of the present invention has a smaller inner diameter and a shorter length, which can reduce the residual drug solution in the injection device; the present invention is designed with a silicone plug at one end of the elastic tube, which can prevent liquid reflux during switching between different injection solutions and reduce the risk of infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of a mesenchymal stem cell exosome ovarian injection device.

[0018] Figure 2 for Figure 1 Enlarged schematic diagram of the puncture needle.

[0019] Among them, 1-puncture needle; 101-needle tip; 102-liquid outlet; 2-elastic tube; 3-syringe; 301-injection port; 302-needle; 4-hollow connecting component; 5-rubber plug; 501-guide hole.

[0020] The same reference numerals in the various drawings represent the same components. DETAILED DESCRIPTION

[0021] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0022] like Figure 1 As shown, the present invention provides a mesenchymal stem cell exosome ovarian injection device, comprising a puncture needle 1, an elastic tube 2 and a syringe 3, wherein the side wall of the puncture needle 1 is provided with a plurality of liquid outlet holes 102, the needle tip 101 of the puncture needle 1 is a sharp solid needle tip, one end of the elastic tube 2 is connected to the puncture needle 1 through a hollow connecting component 4, and the other end is provided with a silicone plug 5, and the end of the silicone plug 5 away from the elastic tube 2 is provided with an inwardly recessed guide hole 501.

[0023] The guide hole 501 is used to guide the needle 302 of the syringe 3 to pierce the silicone plug 5 so that the solution in the syringe 3 can flow into the elastic tube 2. Since the silicone plug 5 is made of silicone, it can be automatically closed after the needle 302 exits the silicone plug 5 to prevent the drug or solution from flowing back and avoid contamination.

[0024] The syringe 3 is a conventional medical syringe, including a syringe barrel, a piston, a needle 302 and a protective cap. One of the characteristics of the syringe 3 is its high precision and ability to accurately control the dosage of the drug. The specific structure will not be described in detail here.

[0025] The elastic tube 2 is a hollow tube, which can deliver the therapeutic drug from the syringe 3 into the hollow connecting component 4. At the same time, the elastic tube 2 is soft and bendable, so that the syringe 3 is not restricted in position during injection and can be placed at will, reducing the work pressure of the doctor. At the same time, the elastic tube 3 has a small inner diameter and a short length, which can reduce the residual drug solution in the injection device.

[0026] The front end of the elastic tube 2 is connected to the end of the hollow connecting component 4 , and a joint can be provided at the connection to prevent leakage. The end of the elastic tube 2 is connected to the injection port 301 of the syringe 3 , and the needle 302 is inserted into the elastic tube 2 .

[0027] In a Figure 2 In the specific embodiment shown, the number of the liquid outlet holes 102 is 6, which are arranged in two rows on two opposite sides of the puncture needle 1, with 3 holes in each row. The multi-hole design can improve the distribution uniformity of the therapeutic drug injection.

[0028] In a specific embodiment, in the same column, the spacing between two adjacent liquid outlet holes 102 is 0.3-1.5 mm (for example, 0.3, 0.5, 0.8, 1.2 mm); the needle body length of the puncture needle 1 is 20-50 mm, preferably 30-40 cm; the outer diameter of the needle body of the puncture needle 1 is 0.5-1.8 mm (for example, 0.8, 1.2, 1.6 mm); the needle tip 101 of the puncture needle 1 is 1-5 mm (for example, 1.5, 3, 4.5 mm) long; the inner diameter of the liquid outlet hole 102 is 0.1-0.3 mm, preferably 0.2 mm. The needle tip of the puncture needle is a solid cone structure, and the angle between its generatrix and the height is ≤30° and >0° (for example, 30°, 20°, 15°); the length of the elastic tube 2 is 10 to 50 cm, preferably 15 cm; the inner diameter of the elastic tube 2 is 0.6 to 1.2 mm, preferably 0.6 to 0.8 mm; the specification of the syringe 3 is 1 to 10 mL.

[0029] When in use, the mesenchymal stem cell exosome solution used for treating POI is injected into the syringe 3 of the present invention, and then the needle 302 is inserted into the guide hole 501 through the needle on the silicone plug 5 and inserted into the elastic tube 2, and the piston of the syringe 3 is pushed to fill the entire device with liquid and discharge the gas in the device. The physician holds the hollow connecting component 4 and performs ovarian puncture under ultrasound guidance, so that the puncture needle 1 is inserted into the ovary of the POI patient, and then the therapeutic drug is pushed into the patient's ovary by pushing the piston of the syringe 3. While keeping the puncture needle 1 in the patient's ovary, the syringe 3 is pulled out from the silicone plug 5, and then physiological saline is sucked into the syringe 3 and re-inserted into the silicone plug 5, and then the therapeutic drug remaining in the device is pushed into the patient's ovary by pushing the piston of the syringe 3. Finally, the puncture needle 1 is pulled out from the patient's ovary to end the treatment process.

Claims

1. A mesenchymal stem cell exosome ovarian injection device, comprising a puncture needle, an elastic tube and a syringe, characterized in that: The side wall of the puncture needle is provided with a plurality of liquid outlet holes, the needle tip of the puncture needle is a sharp solid needle tip, one end of the elastic tube is connected with the puncture needle through a hollow connecting component, and the other end is provided with a silicone plug, and the end of the silicone plug away from the elastic tube is provided with an inwardly recessed guide hole, and the guide hole is used to guide the needle of the syringe to pierce the silicone plug.

2. A mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: There are 6 liquid outlet holes in total, which are arranged in two rows on two opposite sides of the puncture needle.

3. A mesenchymal stem cell exosome ovarian injection device according to claim 2, characterized in that: In the same row, the distance between two adjacent liquid outlet holes is 0.3-1.5 mm.

4. A mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The needle body length of the puncture needle is 20 to 50 mm.

5. A mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The outer diameter of the puncture needle is 0.5-1.8 mm.

6. A mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The needle tip length of the puncture needle is 1 to 5 mm.

7. A mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The inner diameter of the liquid outlet hole is 0.1-0.3 mm.

8. The mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The needle tip of the puncture needle is a solid cone structure, and the angle between its generatrix and the height is ≤30° and >0°.

9. The mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The elastic tube has a length of 10 to 50 cm and an inner diameter of 0.6 to 1.2 mm.

10. The mesenchymal stem cell exosome ovarian injection device according to claim 1, characterized in that: The specification of the syringe is 1 to 10 mL.

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