A composite plant growth regulator and its preparation method

Abstract

The present application relates to the technical field of growth regulators, specifically to a composite plant growth regulator and a preparation method thereof, comprising the following raw materials in parts by weight: 0.3-0.8 parts of 14-hydroxybrassinosteroid, 1-3 parts of thiadiazole, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acids, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel, and 5-8 parts of porous adsorption particles. By combining a variety of raw materials and adopting a specific preparation method, the composite plant growth regulator prepared not only slows down the release rate and effectively regulates plant growth in the long term, but also improves the comprehensive properties of soil such as fertilization, air permeability, and water retention, thereby promoting the growth and development of crops.

Description

Technical Field

[0001] The present application relates to the technical field of growth regulators, and in particular to a composite plant growth regulator and a preparation method thereof. Background Art

[0002] Plant growth regulators are a class of chemical substances that have a regulatory effect on the growth and development of plants. They have chemical properties and physiological effects similar to plant hormones. Their presence can affect and effectively regulate the growth and development of plants, including a series of plant life processes from cell growth and division to rooting, germination, flowering, fruiting, maturity and shedding.

[0003] General plant growth regulators are released quickly after application and can regulate plant growth and development in a short period of time, but they are not effective in the long term and have limited effects on crop growth regulation. After they become ineffective, they need to be applied multiple times at appropriate times, which is not only cumbersome to apply but also easily leads to waste of plant growth regulators.

[0004] Hydrogel-encapsulated plant growth regulators can reduce their release rate and have potential applications in agricultural production. However, existing hydrogels are usually synthetic polymers that are not easily degraded by microorganisms. Large-scale use can affect plant growth, reduce soil aeration and permeability, and cause environmental pollution. Summary of the Invention

[0005] The present application aims to overcome at least one of the defects of the prior art and provide a composite plant growth regulator and a preparation method thereof. By combining a variety of preparation raw materials and adopting a specific preparation method, the composite plant growth regulator prepared not only slows down the release rate and effectively regulates plant growth in the long term, but also can improve the comprehensive properties of the soil such as fertility, air permeability, and water retention, thereby promoting the growth and development of crops.

[0006] In a first aspect, the present invention provides a composite plant growth regulator, which is implemented by the following technical solutions:

[0007] A composite plant growth regulator comprises the following raw materials in parts by weight:

[0008] 0.3-0.8 parts of 14-hydroxybrassinosteroid, 1-3 parts of thidiazuron, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acids, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel, and 5-8 parts of porous adsorption particles;

[0009] The preparation method of the degradable hydrogel comprises the following steps: first, dissolving 8-12 parts of sulfonated modified lignin and 7-9 parts of chitosan by weight in a calcium chloride solution with a solute content of 12-15 parts, heating and stirring at 55-65° C. for 15-25 minutes until completely dissolved; after cooling to room temperature, adding 4-5 parts of acrylic acid and 0.8-1.2 parts of ammonium persulfate, bubbling nitrogen and stirring for 4-8 minutes, and finally adding 0.3-0.5 parts of N,N'-methylenebisacrylamide and stirring until completely dissolved to obtain a prepolymer solution, then quickly transferring the prepolymer solution to a polytetrafluoroethylene mold, placing it in an oven at 65-75° C. for crosslinking for 1.5-2.5 hours to obtain the degradable hydrogel.

[0010] The composite plant growth regulator according to the embodiments of the present application has at least the following beneficial effects:

[0011] This application uses sulfonated modified lignin as the matrix and chitosan as the Ca 2+ The connecting units are connected by ionic bonds, and the sulfonated modified lignin and chitosan are connected by chemical cross-linking to form a sulfonated modified lignin-based double network hydrogel, which is a degradable hydrogel. The degradable hydrogel has a denser and richer network structure, which gives it less water loss. Adding chitosan and Ca 2+ Both can improve the water retention properties of hydrogels and can be used to improve the water utilization rate and drought resistance of plants; calcium ions are an important component of plant cell walls and are essential for maintaining the stability of cell structure. They also participate in various signal transduction processes in plants and have an important impact on plant growth and development. Adding an appropriate amount of calcium ions to plant growth regulators can enhance the plant's stress resistance and improve yield and quality.

[0012] The lignin and chitosan in the degradable hydrogel of the present application are both derived from renewable resources, and both lignin and chitosan are biodegradable, so the degradable hydrogel has excellent environmental performance. Lignin has rich active functional groups such as phenolic hydroxyl groups, which give it strong chemical reactivity and bonding properties. After sulfonation modification, the dispersibility and compatibility of lignin in polylactic acid emulsion are improved. After the hydrogel is applied to the plant, its functional groups can agglomerate and bond soil particles through electrostatic adsorption in the soil to form large-size soil particles with a granular structure, effectively improving the soil structure, enhancing the soil particles' resistance to erosion, anti-collapse and water stability, and improving water retention and soil solidification.

[0013] The present application combines porous adsorption particles with a variety of raw materials such as 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid, etc., so that some effective ingredients are adsorbed inside the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers. The raw materials are synergistically matched and promote each other to give full play to the efficacy of each raw material, improve fertility, promote plant growth and development, and after entering the soil, they can also adsorb microorganisms, improve soil structure, increase soil permeability, and facilitate the respiration and growth of plant roots.

[0014] The 14-hydroxybrassinosteroid of the present application has physiological effects such as promoting cell elongation and division, regulating leaf shape, changing cell membrane potential and enzyme activity, and enhancing photosynthesis, thereby significantly improving the growth rate and growth quality of plants. It can also stimulate the activity of various immune enzymes in the plant body, activate the immune system, and thus enhance the plant's resistance to drought, high temperature, frost and other stresses; thiadiazole has extremely strong cell division activity, can delay plant aging, enhance its stress resistance, promote plant photosynthesis, increase crop yield, and improve product quality; triacontanol can increase the effective tillering of crops, promote the healthy growth of seedlings, and can also enhance the respiration of crop plants and promote the absorption and utilization of mineral nutrients by the roots. Using triacontanol for foliar spraying during the initial and peak flowering periods of crops can promote the formation of flower buds, increase flowering pollination and fruit setting rates, and promote flower bud differentiation of fruit trees, increase fruit setting rates, and increase fruit set rates, thereby achieving the purpose of increasing production.

[0015] The seaweed fertilizer in this application refers to a biological fertilizer produced from seaweed or seaweed extracts through fermentation, acid-base process or fertilizer mixing process. Seaweed fertilizer is rich in nutrients and is rich in various minerals such as potassium, calcium, magnesium, iron, zinc, etc. The seaweed polysaccharides, alginate, highly unsaturated fatty acids and various natural plant growth regulators unique to seaweed can stimulate the production of nonspecific active factors in plants and regulate the balance of endogenous hormones. It can also improve soil, enhance crop photosynthesis and improve crop stress resistance.

[0016] The fulvic acid of the present application has a small molecular weight and is easily absorbed and utilized by organisms. It can reduce the opening of crop stomata, reduce water transpiration, and increase the activity of various enzymes and chlorophyll content, increase metabolism and photosynthesis, improve crop resistance to frost and disease, increase crop yield, improve crop quality, and can also chelate trace elements and improve the crop's ability to absorb trace elements.

[0017] According to some embodiments of the present application, the method for preparing the porous adsorption particles comprises the following steps:

[0018] S1. The rapeseed pollen, Fe3O4 powder, nano-zinc oxide was added to the silane coupling agent aqueous solution, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0019] S2 weighed 30-40 parts by weight of clay, 4-8 parts by weight of ammonium bicarbonate, 35-50 parts by weight of the composite powder, mixed uniformly, added 25-35 parts by weight of water, granulated to form composite particles;

[0020] S3. The composite particles are preheated to 105-115°C, then calcined at 500-700°C, cooled, and ground to form porous adsorption particles.

[0021] Rapeseed pollen is abundant in nature, has good biocompatibility, is easily degraded in the later stage, and has strong adsorption properties in the porous material after sintering. Nano zinc oxide, as a nanomaterial, has high biological activity, high absorption rate, strong antioxidant capacity, safety and stability. Adding nano zinc oxide to porous adsorption particles can supplement the plant's demand for zinc, participate in various metabolic processes, including protein synthesis, carbohydrate metabolism and redox reactions, improve the plant's stress resistance, promote root development and nutrient absorption, and thus promote plant growth and development.

[0022] Furthermore, in step S1, the weight ratio of the rapeseed pollen to the Fe3O4 powder and the nano zinc oxide is (1-3):1:1.

[0023] Furthermore, the silane coupling agent in step S1 is γ-aminopropyltriethoxysilane.

[0024] Furthermore, after the grinding in step S3, a pressurized spray treatment using sodium hexadecyl diphenyl oxide disulfonate is also performed. In the molecular structure of sodium hexadecyl diphenyl oxide disulfonate, the long alkyl chain provides hydrophobicity, the ether bond connecting the two benzene rings increases the stability and compatibility of the molecule, and the sulfonic acid group provides hydrophilicity and ionicity, enhancing solubility in water. This molecular structure gives sodium hexadecyl diphenyl oxide disulfonate excellent dispersibility, allowing it to effectively disperse porous adsorption particles in liquid media.

[0025] Furthermore, the amount of the sodium hexadecyl diphenyl ether disulfonate is 1-2 wt % of the weight of the porous adsorption particles.

[0026] According to some embodiments of the present application, the porous adsorption particles have a particle size of 1-3 μm.

[0027] According to some embodiments of the present application, the method for preparing the sulfonated modified lignin comprises the following steps:

[0028] Lignin and Na2SO3 are evenly mixed in a weight ratio of (3-5):(2-3), and a NaOH solution with a mass fraction of 15% is added, and the mixture is reacted at a temperature of 150-200°C for 3-5 hours to obtain sulfonated modified lignin.

[0029] The reaction temperature, reaction time and Na2SO3 dosage in the preparation of sulfonated modified lignin are carefully set. For example, too high a reaction temperature may lead to lignin degradation, while too much Na2SO3 may increase the cost and reduce the purity of the product.

[0030] According to some embodiments of the present application, the amino acid is selected from at least one of alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

[0031] In a second aspect, the present invention provides a method for preparing the composite plant growth regulator, which is achieved by the following technical solutions:

[0032] The preparation method of the composite plant growth regulator comprises the following steps:

[0033] 14-hydroxybrassinosteroid, thiadipyridin, triacontanol, seaweed fertilizer, amino acid, and fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment according to weight parts, and the degradable hydrogel is stirred at a speed of 80-100 r / min while pouring. Porous adsorption particles are added and mixed evenly to obtain a composite plant growth regulator.

[0034] The preparation method of the composite plant growth regulator according to the embodiment of the present application has at least the following beneficial effects:

[0035] The preparation method of the present application has simple steps. By fully mixing 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid, porous adsorption particles and degradable hydrogel, some effective ingredients are adsorbed inside by the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers; after the composite plant growth regulator is sprayed onto the plant surface, due to the action of the degradable hydrogel, the release rate of the plant growth regulator can be further slowed down, promoting plant growth for a long time, and can also store part of the water, reduce water evaporation on the plant surface, and replenish the water required for plant growth; at the same time, the preparation method of the present application does not require the use of complex production equipment, has low cost, is pollution-free in the manufacturing process, does not emit toxic substances, does not harm the health of operators, and is suitable for industrial production. DETAILED DESCRIPTION

[0036] To make the purpose, technical solutions and advantages of this application more clear, the following will be further described in detail with reference to specific embodiments. The embodiments described here are only part of the embodiments of this application and should not be understood as limiting the scope of protection of this application.

[0037] Example 1

[0038] Preparation of compound plant growth regulator:

[0039] (1) Preparation of sulfonated modified lignin:

[0040] Lignin and Na2SO3 were mixed evenly in a weight ratio of 4:2.5, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0041] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0042] (3) Preparation of porous adsorption particles:

[0043] S1 according to a weight ratio of 2:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0044] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed uniformly, added 30 parts by weight of water, granulated to form composite particles;

[0045] S3. The composite particles are preheated to 110°C, then calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0046] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5 wt% of the weight of the porous adsorption particles;

[0047] (4) According to weight parts, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90r / min while pouring, and 6 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0048] Example 2

[0049] Preparation of compound plant growth regulator:

[0050] (1) Preparation of sulfonated modified lignin:

[0051] Lignin and Na2SO3 were mixed uniformly in a weight ratio of 5:2, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 200°C for 3 hours to obtain sulfonated modified lignin;

[0052] (2) Preparation of degradable hydrogel: First, 12 parts of sulfonated modified lignin and 7 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 15 parts according to weight, and heated and stirred at 55°C for 25 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1.2 parts of ammonium persulfate were added, nitrogen was bubbled and stirred for 4 minutes, and finally 0.5 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 65°C for crosslinking for 2.5 hours to obtain a degradable hydrogel;

[0053] (3) Preparation of porous adsorption particles:

[0054] S1 according to a weight ratio of 1:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0055] S2 weighed 40 parts by weight of clay, 4 parts by weight of ammonium bicarbonate, 50 parts by weight of the composite powder, mixed uniformly, added 25 parts by weight of water, granulated to form composite particles;

[0056] S3. The composite particles were preheated to 115°C, then calcined at 500°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0057] The amount of sodium hexadecyl diphenyl ether disulfonate is 2 wt% of the weight of the porous adsorption particles;

[0058] (4) According to weight parts, 0.3 parts of 14-hydroxybrassinosteroid, 3 parts of thiadiazole, 2 parts of triacontanol, 8 parts of seaweed fertilizer, 1 part of glutamic acid, and 3 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 30 parts of the degradable hydrogel is stirred at a speed of 80 r / min while pouring, and 5 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0059] Example 3

[0060] Preparation of compound plant growth regulator:

[0061] (1) Preparation of sulfonated modified lignin:

[0062] Lignin and Na2SO3 were mixed uniformly in a weight ratio of 3:3, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 150°C for 5 hours to obtain sulfonated modified lignin;

[0063] (2) Preparation of degradable hydrogel: First, 8 parts of sulfonated modified lignin and 9 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 12 parts according to weight, and heated and stirred at 65°C for 15 minutes until completely dissolved; after cooling to room temperature, 5 parts of acrylic acid and 0.8 parts of ammonium persulfate were added, nitrogen was bubbled and stirred for 8 minutes, and finally 0.3 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 75°C for crosslinking for 1.5 hours to obtain a degradable hydrogel;

[0064] (3) Preparation of porous adsorption particles:

[0065] S1 according to a weight ratio of 3:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0066] S2 weighed 30 parts by weight of clay, 8 parts by weight of ammonium bicarbonate, 35 parts by weight of the composite powder, mixed uniformly, added 35 parts by weight of water, granulated to form composite particles;

[0067] S3. The composite particles are preheated to 105°C, then calcined at 700°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0068] The amount of sodium hexadecyl diphenyl ether disulfonate is 1 wt% of the weight of the porous adsorption particles;

[0069] (4) According to weight parts, 0.8 parts of 14-hydroxybrassinosteroid, 1 part of thiadiazole, 4 parts of triacontanol, 5 parts of seaweed fertilizer, 1 part of glycine, 1 part of lysine, 1 part of phenylalanine, and 1 part of fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, and 20 parts of the degradable hydrogel are stirred at a speed of 100 r / min while pouring, and 8 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0070] Example 4

[0071] Preparation of compound plant growth regulator:

[0072] (1) Preparation of sulfonated modified lignin:

[0073] Lignin and Na2SO3 were mixed uniformly in a weight ratio of 4:3, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 170°C for 4 hours to obtain sulfonated modified lignin;

[0074] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 5 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0075] (3) Preparation of porous adsorption particles:

[0076] S1 according to a weight ratio of 2:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0077] S2 weighed 35 parts by weight of clay, 5 parts by weight of ammonium bicarbonate, 40 parts by weight of the composite powder, mixed uniformly, added 30 parts by weight of water, granulated to form composite particles;

[0078] S3. The composite particles are preheated to 110°C, then calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0079] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5 wt% of the weight of the porous adsorption particles;

[0080] (4) According to weight parts, 0.6 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 7 parts of seaweed fertilizer, 2 parts of tyrosine, and 1 part of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 900 r / min while pouring, and 6 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0081] Comparative Example 1

[0082] Preparation of compound plant growth regulator:

[0083] (1) Preparation of sulfonated modified lignin:

[0084] Lignin and Na2SO3 were mixed evenly in a weight ratio of 4:2.5, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0085] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in water according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0086] (3) Preparation of porous adsorption particles:

[0087] S1 according to a weight ratio of 2:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0088] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed uniformly, added 30 parts by weight of water, granulated to form composite particles;

[0089] S3. The composite particles are preheated to 110°C, then calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0090] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5 wt% of the weight of the porous adsorption particles;

[0091] (4) According to weight parts, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90r / min while pouring, and 6 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0092] Comparative Example 2

[0093] Preparation of compound plant growth regulator:

[0094] (1) Preparation of degradable hydrogel: First, 10 parts of lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0095] (2) Preparation of porous adsorption particles:

[0096] S1 according to a weight ratio of 2:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0097] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed uniformly, added 30 parts by weight of water, granulated to form composite particles;

[0098] S3. The composite particles are preheated to 110°C, then calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0099] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5 wt% of the weight of the porous adsorption particles;

[0100] (3) According to weight parts, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90r / min while pouring, and 6 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0101] Comparative Example 3

[0102] Preparation of compound plant growth regulator:

[0103] (1) Preparation of sulfonated modified lignin:

[0104] Lignin and Na2SO3 were mixed evenly in a weight ratio of 4:2.5, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0105] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0106] (3) According to weight parts, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90r / min while pouring. After mixing evenly, the composite plant growth regulator is obtained.

[0107] Comparative Example 4

[0108] Preparation of compound plant growth regulator:

[0109] (1) Preparation of sulfonated modified lignin:

[0110] Lignin and Na2SO3 were mixed evenly in a weight ratio of 4:2.5, and a 15% mass fraction of NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0111] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0112] (3) Preparation of porous adsorption particles:

[0113] S1 according to a weight ratio of 2:1:1 rape pollen, Fe3O4 powder and nano-zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0114] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed uniformly, added 30 parts by weight of water, granulated to form composite particles;

[0115] S3. The composite particles are preheated to 110°C, then calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm.

[0116] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5 wt% of the weight of the porous adsorption particles;

[0117] (4) According to weight, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a dark environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90 r / min while pouring, and 6 parts of porous adsorption particles are added. After mixing evenly, a composite plant growth regulator is obtained.

[0118] Experimental example

[0119] Pea plants with similar growth conditions and a plant height of 5 cm were selected and planted in 8 different farmlands in the same village. 20 pea plants were planted in each farmland. The 8 farmlands were sprayed with the composite plant growth regulators prepared in Examples 1-4 and Comparative Examples 1-4, respectively. The plants were watered once a week. The soil moisture content was measured before the last watering for 3 weeks. The plant height was tested after the last watering for 3 weeks. The photosynthesis rate of the leaves was tested using a portable photosynthesis meter at 1:00 p.m. the next day. Healthy leaves from similar parts of the plants were selected for the test. The measured data for the peas planted in each farmland were averaged. The results are shown in Table 1.

[0120] Table 1

[0121]

[0122] As can be seen from Table 1, the composite plant growth regulators prepared in Examples 1-4 of the present application, after being sprayed on pea fields, can promote the growth of pea plants, accelerate the photosynthesis rate of the plants, and increase the moisture content in the soil.

[0123] Calcium chloride was not used in the preparation of the degradable hydrogel in Comparative Example 1. The rest was the same as in Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 1 was significantly inferior to that in Example 1 of the present application. The height of pea plants and the photosynthesis rate of leaves were significantly reduced. This shows that the present application uses sulfonated modified lignin as the matrix and chitosan as the matrix to react with CaCl2. 2+ The connecting units are connected by ionic bonds, and the sulfonated modified lignin and chitosan are connected by chemical cross-linking to form a sulfonated modified lignin-based double network hydrogel, which is a degradable hydrogel. The degradable hydrogel has a denser and richer network structure, which gives it less water loss. Adding chitosan and Ca 2+Both can improve the water retention properties of hydrogels and can be used to improve the water utilization rate and drought resistance of plants; calcium ions are an important component of plant cell walls and are essential for maintaining the stability of cell structure. They also participate in various signal transduction processes in plants and have an important impact on plant growth and development. Adding an appropriate amount of calcium ions to plant growth regulators can enhance the plant's stress resistance and improve yield and quality.

[0124] The lignin in the raw material of the degradable hydrogel of Comparative Example 2 is sulfonated, and the rest are the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 2 is significantly inferior to that of Example 1 of the present application. The pea plant height, leaf photosynthesis rate and soil moisture content are significantly reduced, indicating that lignin has rich active functional groups such as phenolic hydroxyl groups, which give it strong chemical reactivity and bonding properties. After sulfonation modification, the dispersibility and compatibility of lignin in polylactic acid emulsion are improved. After the hydrogel is applied to the plant, its functional groups in the soil can agglomerate and bond soil particles through electrostatic adsorption to form large-particle soil particles with a granular structure, effectively improving the soil structure, enhancing the anti-erosion, anti-disintegration and water stability of the soil particles, and improving the water retention and soil solidification properties.

[0125] Porous adsorption particles were not used in the preparation raw materials of Comparative Example 3, and the rest were the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 3 was significantly inferior to that of Example 1 of the present application. The pea plant height, leaf photosynthesis rate and soil moisture content were significantly reduced, indicating that the present application combines porous adsorption particles with 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid and other raw materials. Part of the active ingredients are adsorbed into the interior by the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers. The raw materials are synergistically matched and promote each other to give full play to the efficacy of each raw material, improve fertility, promote plant growth and development, and can also adsorb microorganisms after entering the soil, improve soil structure, increase soil permeability, and facilitate the respiration and growth of plant roots.

[0126] 14-hydroxybrassinosteroid was not used in the preparation raw materials of Comparative Example 4, and the rest were the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 4 was significantly inferior to that of Example 1 of the present application. The plant height and leaf photosynthesis rate of peas were significantly reduced, indicating that the 14-hydroxybrassinosteroid, thiadiazole, and triacontanol of the present application are mutually complementary and indispensable. 14-hydroxybrassinosteroid has physiological effects such as promoting cell elongation and division, regulating leaf shape, changing cell membrane potential and enzyme activity, and enhancing photosynthesis, thereby significantly improving the growth rate and growth quality of plants. It can also stimulate the activity of various immune enzymes in the plant body and activate the immune system, thereby enhancing the plant's drought resistance, high temperature resistance, frost resistance and other stress resistance.

[0127] Although the embodiments of the present application have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions or variations may be made to these embodiments without departing from the principles and purpose of the present application, and that the technical solutions after these changes, modifications, substitutions or variations will fall within the scope of protection of the present application.

Claims

1. A composite plant growth regulator, characterized in that: The preparation comprises the following raw materials in parts by weight: 0.3-0.8 parts of 14-hydroxybrassinosteroid, 1-3 parts of thidiazuron, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acids, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel, and 5-8 parts of porous adsorption particles; The preparation method of the degradable hydrogel comprises the following steps: first, dissolving 8-12 parts of sulfonated modified lignin and 7-9 parts of chitosan by weight in a calcium chloride solution having a solute content of 12-15 parts, heating and stirring at 55-65° C. for 15-25 minutes until completely dissolved; after cooling to room temperature, adding 4-5 parts of acrylic acid and 0.8-1.2 parts of ammonium persulfate, bubbling nitrogen and stirring for 4-8 minutes, and finally adding 0.3-0.5 parts of N,N'-methylenebisacrylamide and stirring until completely dissolved to obtain a prepolymer solution, then quickly transferring the prepolymer solution to a polytetrafluoroethylene mold, placing the mold in an oven at 65-75° C. for crosslinking for 1.5-2.5 hours to obtain the degradable hydrogel; The method for preparing the porous adsorption particles comprises the following steps: S1. The rapeseed pollen, Fe3O4 powder, nano-zinc oxide was added to the silane coupling agent aqueous solution, ultrasonicated and stirred, and then dried and ground to form a composite powder; S2 weighed 30-40 parts by weight of clay, 4-8 parts by weight of ammonium bicarbonate, 35-50 parts by weight of the composite powder, mixed uniformly, added 25-35 parts by weight of water, granulated to form composite particles; S3. The composite particles are preheated to 105-115 ℃, then calcined at 500-700 ℃, cooled, ground, and prepared into porous adsorption particles; In step S1, the weight ratio of the rapeseed pollen to the Fe3O4 powder and the nano zinc oxide is (1-3):1:1; The preparation method of the sulfonated modified lignin comprises the following steps: Lignin and Na2SO3 are evenly mixed in a weight ratio of (3-5):(2-3), and a NaOH solution with a mass fraction of 15% is added, and the mixture is reacted at a temperature of 150-200°C for 3-5 hours to obtain sulfonated modified lignin.

2. A composite plant growth regulator according to claim 1, characterized in that: After the grinding in step S3, the process further includes performing a pressurized spray treatment using sodium hexadecyl diphenyl ether disulfonate.

3. A composite plant growth regulator according to claim 2, characterized in that: The amount of the sodium hexadecyl diphenyl ether disulfonate is 1-2 wt % of the weight of the porous adsorption particles.

4. A composite plant growth regulator according to claim 1, characterized in that: The silane coupling agent in step S1 is γ-aminopropyltriethoxysilane.

5. A composite plant growth regulator according to claim 1, characterized in that: The particle size of the porous adsorption particles is 1-3 μm.

6. A composite plant growth regulator according to claim 1, characterized in that: The amino acid is selected from at least one of alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

7. A method for preparing the composite plant growth regulator according to any one of claims 1 to 6, characterized in that: The following steps are involved: 14-hydroxybrassinosteroid, thiadipyridin, triacontanol, seaweed fertilizer, amino acid, and fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment according to weight parts, and the degradable hydrogel is stirred at a speed of 80-100 r / min while pouring. Porous adsorption particles are added and mixed evenly to obtain a composite plant growth regulator.

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