Chromaticity detection instrument based on isothermal amplification

Abstract

The invention discloses an isothermal amplification-based chromaticity detection instrument which comprises a fixing seat for fixing a kit and a power supply unit for supplying power, a light source is arranged on one side of the fixing seat, a light signal sensor corresponding to the light source is arranged on the other side of the fixing seat, a heating body is arranged in the fixing seat, and the power supply unit is used for supplying power to the light source, the light signal sensor and the heating body. A plurality of light path through holes are formed in the fixing seat in the direction that the light source directly faces the light signal sensor, the light path through holes directly face the kit reaction cavity, the light source is connected with light source circuits with the number corresponding to that of the light path through holes, each light source circuit comprises a light-emitting diode and an adjustable resistor which are connected in series, and the light source circuits are connected in parallel. The resistance of the light source circuit is independently adjusted through the adjustable resistor, the current intensity of the circuit is adjusted, the light emitting intensity of the light source is adjusted, the consistency of the emitted light intensity of all the light path through holes is achieved through one-by-one adjustment, the reliability of the detection result is ensured, and the accuracy of the detection result can be effectively improved.

Description

Technical Field

[0001] The invention relates to the field of detection devices, in particular to a colorimetric detection instrument based on isothermal amplification. Background Art

[0002] Loop-mediated isothermal amplification (LAMP) is a new in vitro nucleic acid amplification detection technology established by Notomi in 2001. It uses 4-6 primers to identify 6-8 specific nucleic acid fragments. DNA polymerase has a chain displacement function. With its help, the target gene can be amplified efficiently and specifically in a very short time under constant temperature conditions. At the same time, a large amount of white magnesium pyrophosphate precipitate is precipitated in the reaction. Using this feature, the experimenter can judge whether the result is negative or positive by observing the turbidity of the reaction system. Since the LAMP reaction is carried out under constant temperature conditions, a stable heat source (such as a water bath or a metal bath) can meet the requirements of the experimental conditions. The whole process is monitored by the instrument, which can eliminate the interference of human judgment. The results are credible and can truly reproduce the time point of the LAMP reaction, reaction efficiency and other parameters. It has a very good guiding significance for the best primer screening experiment and the best temperature experiment. It is an indispensable instrument for the current research of LAMP kits. With the increasing popularity of multiple joint detection, how to ensure the consistency of the fluorescence intensity reflected by each amplification chamber is crucial. Summary of the invention

[0003] In view of the above problems, the present invention provides a chromaticity detection module, which has the advantage of being able to adjust the light intensity, ensure that the output light signals of multiple amplification cavities are consistent, and provide detection reliability.

[0004] The technical solution of the present invention is:

[0005] A colorimetric detection instrument based on isothermal amplification comprises a fixing seat for fixing a reagent box and a power supply unit for powering. A light source is arranged on one side of the fixing seat, and a light signal sensor corresponding to the light source is arranged on the other side. A heating body is built in the fixing seat. The power supply unit is used to supply power to the light source, the light signal sensor and the heating body. The fixing seat is provided with a plurality of light path through holes along the direction opposite to the light source and the light signal sensor, the light path through holes are opposite to the reaction chamber of the reagent box, the light source is connected to a light source circuit corresponding to the number of light path through holes, each of the light source circuits comprises a light emitting diode and an adjustable resistor connected in series, and a plurality of the light source circuits are connected in parallel.

[0006] A temperature sensor is arranged at the optical signal sensor.

[0007] A light guide column is coaxially arranged at each of the optical path through holes.

[0008] A protection resistor is connected in series to each light source circuit.

[0009] A normally closed switch is connected in series to each light source circuit.

[0010] Each light source circuit is connected in parallel with a detachable constant resistor.

[0011] The optical path through holes are no less than five.

[0012] The beneficial effects of the present invention are:

[0013] The light source circuit corresponding to each optical path through hole can use an adjustable resistor to independently adjust the resistance. The light intensity of one of the optical path through holes is determined as a benchmark, and the resistance values ​​of the adjustable resistors on the remaining light source circuits are adjusted in turn, thereby adjusting the current intensity of the circuit and then adjusting the luminous intensity of the light source, thereby achieving consistency in the light intensity emitted by all optical path through holes, ensuring that the detection results are reliable, and effectively improving the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the use status of a colorimetric detection instrument based on isothermal amplification according to an embodiment of the present invention;

[0015] Figure 2 It is a schematic diagram of the structure of a detection module of a colorimetric detection instrument based on isothermal amplification according to an embodiment of the present invention;

[0016] Figure 3 It is a circuit diagram of a light source circuit of a colorimetric detection instrument based on isothermal amplification according to an embodiment of the present invention.

[0017] Description of reference numerals:

[0018] 1 is a fixing seat, 2 is a power supply unit, 3 is a light source, 4 is a light signal sensor, 5 is a reagent box, 11 is a heating body, 31 is a light source circuit, 311 is a light source, 312 is an adjustable resistor, 313 is a protective resistor, and 314 is a constant resistor. DETAILED DESCRIPTION

[0019] The embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0020] Example:

[0021] like Figure 1-Figure 3As shown, a colorimetric detection instrument based on isothermal amplification includes a fixing seat 1 for fixing a reagent kit and a power supply unit 2 for powering. A light source 3 is arranged on one side of the fixing seat 1, and an optical signal sensor 4 corresponding to the light source is arranged on the other side. The fixing seat 1 has a built-in heating body 11, and the power supply unit 2 is used to supply power to the light source 3, the optical signal sensor 4, and the heating body 11. The fixing seat 1 is provided with a plurality of optical path through holes along the direction opposite to the light source 3 and the optical signal sensor 4, and the optical path through holes are opposite to the reaction chamber of the reagent kit. The light source 3 is connected to light source circuits 31 corresponding to the number of optical path through holes, and each light source circuit 31 includes a light emitting diode 311 and an adjustable resistor 312 connected in series, and a plurality of light source circuits 31 are connected in parallel with each other.

[0022] The working principle of the above technical solution is as follows:

[0023] The fixing seat 1 is used to accommodate a detection kit containing a detection sample. The power supply unit 2 supplies power to the light source 3 on one side of the fixing seat 1, the optical signal sensor 4 on the other side, and the built-in heating body 11 of the fixing seat 1. The heating body 11 raises the temperature in the fixing seat 1 to the amplification temperature to ensure that the reagent kit 5 placed in the fixing seat 1 can undergo an amplification reaction. The light source 311 emits light along the optical path through hole to illuminate the reaction chamber of the reagent kit. After the light penetrates the reaction chamber of the reagent kit, it is received by the optical signal sensor 4. Since there are multiple optical path through holes for multiple detection items, in order to ensure that the light intensity signal received by the optical signal sensor 4 is consistent, the light source circuit 31 corresponding to each optical path through hole can use an adjustable resistor 312 to independently adjust the resistance. The light intensity of one of the optical path through holes is determined as a reference, and the resistance values ​​of the adjustable resistors 312 on the remaining light source circuits 31 are adjusted in turn, thereby adjusting the current intensity of the circuit and then adjusting the luminous intensity of the light source, thereby achieving consistency in the light intensity emitted by all optical path through holes, ensuring that the detection results are reliable, and effectively improving the accuracy of the detection results.

[0024] A temperature sensor is arranged at the optical signal sensor 4 to monitor the amplification temperature and ensure the smooth progress of the amplification reaction.

[0025] A light guide column is coaxially arranged at each optical path through hole 5 to ensure that the light penetrates the amplification cavity along the light guide column, thereby preventing the light from being dispersed and affecting the detection result.

[0026] A protective resistor 313 is connected in series to each light source circuit 31 to prevent the normal operation of the circuit from being affected when the adjustable resistor fails.

[0027] Each light source circuit 31 is connected in series with a normally closed switch, and the number of light sources can be controlled according to the needs of the detection items. Each light source circuit 31 is connected in parallel with a detachable constant resistor 314, and the constant resistor 314 and the adjustable resistor 312 can be switched to improve the flexibility of detection.

[0028] There are no less than five optical path holes, which can be used for multiple inspection projects. Some of the optical path holes can be opened or closed as needed.

[0029] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A colorimetric detection instrument based on isothermal amplification, comprising a fixing seat for fixing a reagent kit and a power supply unit for powering, a light source is arranged on one side of the fixing seat, and an optical signal sensor corresponding to the light source is arranged on the other side, a heating body is built in the fixing seat, and the power supply unit is used to supply power to the light source, the optical signal sensor, and the heating body, characterized in that: The fixing seat is provided with a plurality of light path through holes along the direction opposite to the light source and the optical signal sensor, the light path through holes are opposite to the reaction chamber of the reagent kit, the light source is connected to a light source circuit corresponding to the number of light path through holes, each of the light source circuits includes a light emitting diode and an adjustable resistor connected in series, and a plurality of the light source circuits are connected in parallel.

2. A colorimetric detection instrument based on isothermal amplification according to claim 1, characterized in that: A temperature sensor is arranged at the optical signal sensor.

3. A colorimetric detection instrument based on isothermal amplification according to claim 1, characterized in that: A light guide column is coaxially arranged at each of the optical path through holes.

4. A colorimetric detection instrument based on isothermal amplification according to claim 1, characterized in that: A protection resistor is connected in series to each light source circuit.

5. A colorimetric detection instrument based on isothermal amplification according to claim 4, characterized in that: A normally closed switch is connected in series to each light source circuit.

6. A colorimetric detection instrument based on isothermal amplification according to claim 5, characterized in that: Each light source circuit is connected in parallel with a detachable constant resistor.

7. A colorimetric detection instrument based on isothermal amplification according to claim 1, characterized in that: The optical path through holes are no less than five.

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