A bionic vaginal fluid with moisturizing and anti-inflammatory effects and a preparation method and application thereof

Abstract

The present application belongs to the technical field of raw materials for female and gender external products, and particularly relates to a bionic vaginal fluid with moisturizing and anti-inflammatory effects and a preparation method and application thereof. The bionic vaginal fluid comprises the following components in parts by weight: polysaccharide of Phaeolepiota aurea 0.1-2 parts and succinyl polysaccharide 0.1-1 part. The present application innovatively uses the combination of polysaccharide of Phaeolepiota aurea and succinyl polysaccharide to prepare the bionic vaginal fluid. The two components cooperate and interact with each other, can play a synergistic effect, and can significantly improve the moisturizing property and anti-inflammatory soothing effect of the bionic vaginal fluid, can provide lubrication and good moisturizing feeling for the vagina, and at the same time help maintain the microecological balance in the vagina and improve the immune resistance of the vagina.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of raw materials for female and bisexual external products, and particularly relates to a bionic vaginal fluid with moisturizing and anti-inflammatory effects and a preparation method and application thereof. BACKGROUND

[0002] The existing bionic vaginal fluid or other bionic body fluid products mainly imitate the composition of vaginal secretions and add bactericides, thickeners and other corresponding components to achieve the corresponding purposes and effects. Owen DH and Katz DF provide a specific formula of a vaginal secretion simulator: NaCl 3.51%; KOH 1.40%; Ca(OH)2 0.222%; bovine serum albumin 0.018%; lactic acid 2.00%; acetic acid 1.00%; glycerol 0.16%; urea 0.4%; glucose 5.0%. This developed liquid is based on the existing analysis of the composition of human vaginal secretions, thereby simulating human vaginal fluid. CN103126917B discloses a bionic gynecological care fluid and a preparation method thereof. In this patent, artificial body fluid is used as a solvent, and antibacterial peptides are selected as bactericides, thereby obtaining an artificial vaginal secretion that can treat vaginitis.

[0003] However, the existing bionic vaginal fluid or other bionic body fluid only simply imitates the vaginal fluid, and the moisturizing effect is weak. Moreover, some bionic vaginal fluids need to add bactericides, thickeners and other components to achieve specific effects, which not only may destroy the vaginal microecological balance, but also may cause damage to the vaginal mucosa after long-term and repeated use, thereby affecting the vaginal health and causing vaginal dryness and other problems. SUMMARY

[0004] The present application aims to overcome the deficiencies of the prior art and provide a bionic vaginal fluid with moisturizing and anti-inflammatory effects and a preparation method and application thereof, so as to play the effects of shrinking, moisturizing, soothing and anti-inflammatory, thereby providing lubrication and good moisturizing to the vagina, helping to maintain the microecological balance in the vagina and improving the immune resistance of the vagina.

[0005] To achieve the above-mentioned purposes, the technical solutions adopted by the present application include:

[0006] In a first aspect, the present application provides a bionic vaginal fluid with moisturizing and anti-inflammatory effects, which comprises the following components by weight: 0.1-2 parts of small-spored pholiota polysaccharide and 0.1-1 parts of succinyl polysaccharide.

[0007] The human body's first line of defense consists of the skin and mucous membranes and their secretions, which can block most pathogens from invading the body. The main component of the mucosal barrier is mucin, and the basic structure of mucin is glycoprotein. The vaginal mucosa itself also secretes a fluid rich in mucin as a lubricant. The microsporum polysaccharide used in this invention is extracted from Pleurotus eryngii and is a component of the plant's secreted mucus barrier. It is a unique polysaccharide similar to the biological mucosal protective component mucin. This biomolecule, composed of proteins and sugars, can protect the mucosal cell layer in contact with the external air layer from dryness and external stimuli, and has both moisturizing and barrier functions. At the same time, as a polysaccharide, it has the effect of prebiotics and can regulate the vaginal microecology. In addition, microsporum polysaccharide has a slippery moisturizing effect, and its water retention effect is 6 times that of hyaluronic acid, with an extremely high water retention rate. It not only has a good moisturizing effect, but also has excellent anti-inflammatory and soothing effects.

[0008] Succinyl polysaccharide is an extracellular polysaccharide composed of glucose and galactose produced by fermentation. It is an extracellular active polysaccharide that also has moisturizing effects. At the same time, it can reduce the response of inflammatory factors, enhance the immune activity of epidermal cells, and thus enhance the skin barrier's resistance.

[0009] Experimental research has revealed that this invention innovatively uses a combination of Microsporum polysaccharide and succinopolysaccharide to prepare a biomimetic vaginal solution. The two components work together and interact with each other to exert a synergistic effect, significantly improving the moisturizing and anti-inflammatory soothing effects of the biomimetic vaginal solution without excessively altering the vaginal microenvironment or disrupting the physiological balance of the vagina. It can be used for daily vaginal care.

[0010] Furthermore, when the inventors investigated the dosage ratio of the two components, they found that when the ratio of Microsporum polysaccharide and succinopolysaccharide exceeded the above-mentioned preferred range, the synergistic effect between the components was not obvious or even antagonistic, which led to a significant decrease in the moisturizing and anti-inflammatory effects of the biomimetic vaginal fluid. This indicates that the two components can only exert an ideal synergistic effect within the scope defined by this invention.

[0011] Preferably, the biomimetic vaginal fluid comprises the following components in parts by weight: 0.5-1 parts of Microsporum polysaccharide and 0.1-0.5 parts of succinyl polysaccharide.

[0012] Preferably, the biomimetic vaginal fluid comprises the following components in parts by weight: 0.6 parts of Microsporum polysaccharide and 0.4 parts of succinyl polysaccharide.

[0013] Experimental research has shown that when Microsporum polysaccharide and succinopolysaccharide are combined in the above-mentioned optimal ratio, the synergistic effect of the two is the best, thereby maximizing the moisturizing, hydrating, soothing and anti-inflammatory effects of the biomimetic vaginal fluid, effectively maintaining the vaginal microecological balance, and improving the vaginal immune resistance.

[0014] Preferably, the mass concentration of the microsporum polysaccharide is 0.5%, and the mass concentration of the succinyl polysaccharide is 1%.

[0015] Preferably, based on 100 parts by weight, the biomimetic vaginal liquid further comprises the following components in parts by weight: 4-6 parts of preservative, 0.6-1.5 parts of moisturizer, 1-3 parts of lactic acid, 0.05-0.15 parts of pH adjuster, and the balance being water.

[0016] Preferably, the biomimetic vaginal fluid further comprises the following components in parts by weight: 4 parts preservative, 1.1 parts moisturizer, 2 parts lactic acid, 0.1 parts pH adjuster, and the balance being water, based on 100 parts by weight.

[0017] Preferably, the preservative comprises 1,2-hexanediol, raspberry ketone, and butylene glycol, wherein the mass ratio of 1,2-hexanediol, raspberry ketone, and butylene glycol is 0.6:0.4:(3-5).

[0018] More preferably, the mass ratio of 1,2-hexanediol, raspberry ketone and butanediol is 0.6:0.4:3.

[0019] Preferably, the moisturizer comprises glycerin, urea, and allantoin, wherein the mass ratio of glycerin, urea, and allantoin is (0.2-0.5):(0.2-0.5):(0.2-0.5).

[0020] More preferably, the mass ratio of glycerol, urea and allantoin is 0.4:0.4:0.3.

[0021] In the preparation of the biomimetic vaginal liquid, this invention adds glycerin, urea, and allantoin as moisturizing ingredients. The three work synergistically to further enhance the moisturizing effect of the biomimetic vaginal liquid and improve the comfort of the vaginal mucosa to a certain extent, providing additional repair and protection, thereby helping the biomimetic vaginal liquid achieve better nursing effects.

[0022] Preferably, the pH adjuster comprises potassium hydroxide.

[0023] Secondly, the present invention provides the application of the aforementioned biomimetic vaginal fluid in the preparation of intimate products for women or both sexes.

[0024] Preferably, the biomimetic vaginal fluid, as a raw material for external products, has a mass percentage of 5-10% in female or sexual intimate products.

[0025] The anti-inflammatory vaginal liquid described in this invention can be applied in cosmetics, disinfectants and other fields, and is especially suitable for use in women's and sexual intimate products. When diluted at 5.0-10%, it is similar to human vaginal secretions in composition, viscosity and pH. It can not only play a moisturizing and anti-inflammatory role, but also enhance the comfort and health of the vaginal area and help maintain the natural barrier function of the vagina.

[0026] Thirdly, the present invention provides a method for preparing the aforementioned biomimetic vaginal fluid, comprising the following steps:

[0027] S1. Heat and stir the preservative at 65-75℃ to obtain the preservative system;

[0028] S2. Add humectant, microsporum polysaccharide, succinyl polysaccharide and water to the preservative system, and stir until the system is clear and transparent;

[0029] S3. Add lactic acid and adjust the pH of the system to 4.0-4.5 using a pH adjuster to obtain the biomimetic vaginal fluid.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0031] This invention innovatively uses a combination of microsporum polysaccharide and succinyl polysaccharide to prepare a biomimetic vaginal fluid. The two components work together and interact with each other to exert a synergistic effect, significantly improving the moisturizing and anti-inflammatory soothing effects of the biomimetic vaginal fluid. This provides the vagina with a lubricating and moisturizing feel while helping to maintain the vaginal microecological balance and enhance the vagina's immune resistance. Attached Figure Description

[0032] Figure 1 The graph shows the effect of the biomimetic vaginal fluid described in Example 3 on the OD value of Lactobacillus curvature.

[0033] Figure 2 The graph shows the effect of the biomimetic vaginal fluid described in Example 3 on the pH value of Lactobacillus curvature. Detailed Implementation

[0034] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0035] Unless otherwise specified, all raw materials used in the following examples and comparative examples are commercially available and commonly used raw materials.

[0036] The microsporum polysaccharide was purchased from Shanghai Yuncui Chemical Technology Co., Ltd., and the succinopolysaccharide was purchased from Shanghai Gefeng Biotechnology Co., Ltd. In the examples and comparative examples, the microsporum polysaccharide was a microsporum polysaccharide solution with a mass concentration of 0.5%, and the succinopolysaccharide was a succinopolysaccharide solution with a mass concentration of 1%.

[0037] Examples 1-5

[0038] Examples 1-5 provide a biomimetic vaginal liquid with moisturizing and anti-inflammatory effects, the specific formula of which is shown in Table 1 (total weight parts: 100 parts). The preparation method of the biomimetic vaginal liquid includes the following steps:

[0039] S1. Heat and stir the preservative at 65-75℃ to obtain the preservative system;

[0040] S2. Add humectant, microsporum polysaccharide, succinyl polysaccharide and water to the preservative system, and stir until the system is clear and transparent;

[0041] S3. Add lactic acid and adjust the pH of the system to 4.0-4.5 using a pH adjuster to obtain the biomimetic vaginal fluid.

[0042] Table 1. Formulation of the biomimetic vaginal fluid described in Examples 1-5 (parts by weight)

[0043]

[0044]

[0045] Comparative Examples 1-5

[0046] The present invention also provides the formulations of the biomimetic vaginal fluids of Comparative Examples 1-5, as shown in Table 2 (total weight parts: 100 parts). The preparation methods of the biomimetic vaginal fluids provided in Comparative Examples 1-5 are similar to those in Examples 1-5, the difference being in the formulation.

[0047] Table 2. Formulation of the biomimetic vaginal fluids described in Comparative Examples 1-5 (parts by weight)

[0048]

[0049]

[0050] Comparative Example 6

[0051] This comparative example provides a biomimetic vaginal fluid, which differs from Example 1 only in that hyaluronic acid is used instead of Microsporum polysaccharide, while the remaining components and amounts are the same as in Example 1.

[0052] Comparative Example 7

[0053] This comparative example provides a biomimetic vaginal fluid, which differs from Example 1 only in that β-glucan is used instead of succinopolysaccharide, while the other components and amounts are the same as in Example 1.

[0054] Example 1

[0055] This example uses the biomimetic vaginal fluids (5% aqueous dilution) prepared in Examples 1-5 and Comparative Examples 1-7 as samples to determine their moisturizing properties. The specific methods are as follows:

[0056] (1) Select the product test area and blank control area on the forearm. Measure the baseline value of the stratum corneum moisture content using a Corneometer.

[0057] (2) Test method: The flexor surface of the forearm was used as the test site, with a test area of ​​3cm*3cm. No product was used in the blank control area. A single, uniform application of (2±0.1)μL / cm of test sample was applied to the product test area. 2 Start timing after application. Measure the stratum corneum moisture content 30 minutes after application using a skin moisture meter (Corneometer); Test conditions: temperature 21℃±1℃, humidity 50%RH±5%RH;

[0058] (3) The moisture content and moisture increase of the stratum corneum were calculated respectively. Each group of samples was repeated three times, and the final result was the average value. The results are shown in Table 3.

[0059] Table 3. Difference in water content of the stratum corneum of the skin

[0060]

[0061]

[0062] As shown in Table 1, the bionic vaginal liquid prepared by the technical solution of the present invention has a good moisturizing effect, thus providing better lubrication and hydration to the vagina. In particular, the bionic vaginal liquid prepared in Example 1 showed a significant increase of 75.69% in the moisture content of the stratum corneum of the skin after testing, and had the best moisturizing effect.

[0063] In Comparative Examples 1-2, when the microsporum polysaccharide and succinopolysaccharide components were missing, the moisturizing effect of the prepared biomimetic vaginal liquid was significantly reduced compared to the examples. In Comparative Examples 4-5, when the ratio of the two components exceeded the preferred range of the present invention, the moisturizing effect of the prepared biomimetic vaginal liquid also decreased. This indicates that the combination of microsporum polysaccharide and succinopolysaccharide described in the present invention can synergistically enhance the moisturizing effect of the biomimetic vaginal liquid, and the best synergistic effect can only be achieved when the dosage of both is within the range defined by the present invention. In Comparative Examples 6-7, when the two components were replaced with other components with similar effects, the moisturizing and water-locking effect of the prepared biomimetic vaginal liquid was also significantly reduced, that is, the synergistic effect was not obvious. Therefore, the microsporum polysaccharide and succinopolysaccharide components in the biomimetic vaginal liquid described in the present invention cannot be replaced by conventional components.

[0064] Example 2

[0065] This study uses the biomimetic vaginal fluid (5% water dilution) prepared in Examples 1-5 and Comparative Examples 1-7 as samples to determine its anti-inflammatory and soothing effects.

[0066] The samples were tested according to laboratory method HMC-WI-029, "Hyaluronase Inhibition Rate". When the hyaluronase inhibition rate of the positive control (1% dipotassium glycyrrhizate) was >50%, the reaction system was considered effective. When the hyaluronase inhibition rate of the sample was significantly higher than that of the negative control (distilled water) (P<0.05), the test sample was considered to have a certain soothing effect. Specific test results are shown in Table 4.

[0067] Table 4 Hyaluronidase Inhibition Rate

[0068]

[0069]

[0070] The hyaluronidase inhibition rate of the sample was significantly higher than that of the negative control (P<0.05), indicating that the test sample had a certain soothing effect; *** indicates that the sample or positive control was significantly different from the negative control (P<0.001); ** indicates that the sample or positive control was significantly different from the negative control (P<0.01).

[0071] As shown in Table 4, the biomimetic vaginal solutions described in Examples 1-5 possess certain soothing and anti-inflammatory effects, effectively improving vaginal mucosal comfort and reducing discomfort or rejection reactions caused by the introduction of foreign substances, thus providing a gentler nursing effect. However, in Comparative Examples 1-7, the soothing and anti-inflammatory effects of the prepared biomimetic vaginal solutions were significantly reduced compared to the examples due to the absence of Microsporum polysaccharide and succinomeric components or when their proportions exceeded the preferred range of this invention.

[0072] Example 3

[0073] This example uses the biomimetic vaginal fluid prepared in Example 1 as a sample to test its effect on the proliferation of Lactobacillus curvature at different concentrations (5% and 8%). The specific methods are as follows:

[0074] In conventional MRS culture media, 5% and 8% of the biomimetic vaginal fluid described in Example 1 were used to replace glucose to prepare experimental culture media. A basal culture medium without a carbon source was used as a blank control group. All the above culture media were adjusted to pH 6.2 ± 0.2, dispensed, and sterilized in an autoclave at 121℃ for 20 min. An appropriate amount of *Lactobacillus curvaturei* lyophilized powder was dissolved in sterile distilled water, spread on plates, and incubated at 37℃ for 24 hours. Single colonies were selected and inoculated into liquid MRS culture medium, incubated at 37℃ for 16 hours. The activated strain was then inoculated into the experimental MRS culture medium at a 1% inoculation rate and incubated at 37℃ for 48 hours. OD values ​​and pH values ​​were measured. OD values ​​were measured at 600 nm using a UV spectrophotometer. OD values ​​indicate the amount of *Lactobacillus curvaturei*; an increase in OD value indicates proliferation. Extensive proliferation of *Lactobacillus curvaturei* produces more extracellular secretions, namely lactic acid; increased lactic acid leads to a decrease in the pH of the system. The specific test results are shown in Table 5.

[0075] Table 5

[0076] Sample origin OD value after 48h incubation pH value after 48h incubation Blank control group 0.1999 5.61 5% biomimetic vaginal fluid 0.3416 4.62 8% biomimetic vaginal fluid 0.3951 4.55

[0077] *Lactobacillus curvatureii* is one of the most common and important probiotics in the vagina, playing a crucial role, particularly in women's vaginal health. (See Table 5 and...) Figures 1-2 The results show that the biomimetic vaginal fluid of the present invention can effectively promote the proliferation of Lactobacillus curvature, thereby helping to maintain and restore the vaginal microecological balance, enhance immune defense, prevent and alleviate various vaginal infections and inflammatory reactions, and effectively maintain vaginal health.

[0078] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A biomimetic vaginal wash with moisturizing and anti-inflammatory effects, characterized in that, Based on 100 parts by weight, the biomimetic vaginal fluid is composed of the following components in parts by weight: 0.6 parts of Microsporum polysaccharide, 0.4 parts of succinyl polysaccharide, 4-6 parts of preservative, 0.6-1.5 parts of moisturizer, 1-3 parts of lactic acid, 0.05-0.15 parts of pH adjuster, and the balance being water; The preservatives include 1,2-hexanediol, raspberry ketone, and butylene glycol, wherein the mass ratio of 1,2-hexanediol, raspberry ketone, and butylene glycol is 0.6:0.4:(3-5). The moisturizer comprises glycerin, urea and allantoin, wherein the mass ratio of glycerin, urea and allantoin is (0.2-0.5):(0.2-0.5):(0.2-0.5).

2. The biomimetic vaginal fluid as described in claim 1, characterized in that, Based on 100 parts by weight, the biomimetic vaginal fluid is composed of the following components in parts by weight: 0.6 parts of Microsporum polysaccharide, 0.4 parts of succinyl polysaccharide, 4 parts of preservative, 1.1 parts of moisturizer, 2 parts of lactic acid, 0.1 parts of pH adjuster, and the balance being water.

3. The method for preparing the biomimetic vaginal fluid as described in claim 1 or 2, characterized in that, Includes the following steps: S1. Heat and stir the preservative at 65-75℃ to obtain the preservative system; S2. Add humectant, microsporum polysaccharide, succinyl polysaccharide and water to the preservative system, and stir until the system is clear and transparent; S3. Add lactic acid and adjust the pH of the system to 4.0-4.5 using a pH adjuster to obtain the biomimetic vaginal fluid.

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