A soothing and repairing composition and its preparation method

Abstract

This invention relates to a soothing and repairing composition and its preparation method, belonging to the field of cosmetic technology. The composition comprises the following raw materials: Polygala tenuifolia root extract, Caprifolium arvense fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, bitter orange oil, β-glucan, hydrogenated rapeseed oil alcohol, and a solvent. This invention utilizes the combined action of Polygala tenuifolia root extract, Caprifolium arvense fruit extract, and bitter orange oil to effectively give the prepared composition an excellent soothing effect; the synergistic effect of β-glucan, Fucus vesiculosus extract, and Artemisia capillaris extract gives the prepared composition an excellent repairing effect. Furthermore, this invention also adds hydrogenated rapeseed oil alcohol to provide moisturizing and hydrating effects, and the added β-glucan, in addition to its repairing effect, can also lock in skin moisture. Therefore, β-glucan can also assist hydrogenated rapeseed oil alcohol in giving the prepared composition a moisturizing and water-locking effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a soothing and repairing composition and its preparation method. Background Technology

[0002] With the fast pace of life and increasing environmental pollution, modern people face more and more skin problems. On the one hand, modern people are often under stress, and coupled with prolonged use of electronic devices and exposure to ultraviolet rays, their skin is prone to discomfort such as sensitivity, redness, and itching, creating an urgent need for soothing skincare products. On the other hand, due to harmful substances in the environment, inappropriate skincare methods, and aging, the skin's barrier function may be impaired. Damaged skin has difficulty retaining moisture and is more susceptible to external stimuli, leading to problems such as sensitivity, redness, damaged skin barrier, and dryness. These problems not only affect skin health but also reduce people's quality of life.

[0003] While some skincare products on the market claim to have soothing, repairing, or moisturizing effects, their results are often unsatisfactory, or they suffer from problems such as limited ingredients, limited efficacy, or poor stability. Therefore, developing a composition with multiple functions including soothing, repairing, and moisturizing has become an important topic in the current skincare industry. Summary of the Invention

[0004] The purpose of this invention is to provide a soothing and repairing composition and its preparation method, which solves the problem that existing compositions have poor soothing, repairing and moisturizing effects.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] A soothing and repairing composition comprising the following ingredients in parts by weight:

[0007] Polygala tenuifolia root extract 0.001-1.5%

[0008] Caprifolia fruit extract 0.1-1%

[0009] Fucus vesiculosus extract 0.1-5%

[0010]

[0011] β-glucan is a polysaccharide composed of glucose molecules linked by β-1,3- and β-1,6- bonds. It can promote the migration of keratinocytes (HaCaT) and skin fibroblasts (HDFa) by regulating intercellular signaling pathways, thereby accelerating the repair and reconstruction of the skin barrier. At the same time, β-glucan can also adsorb and lock in moisture on the skin surface, preventing moisture evaporation and having good moisturizing ability, thus maintaining the skin's hydration.

[0012] The raw material for the root extract of Polygala tenuifolia is the dried root bark of Polygala tenuifolia, a herbaceous plant belonging to the Polygalaceae family and the Polygala genus. The root extract of Polygala tenuifolia can inhibit cell degranulation to a certain extent, reduce the secretion of β-aminohexosidase and histamine, thereby inhibiting the production of cytokines and alleviating the body's allergic reaction.

[0013] Capers are plants belonging to the Capernaum genus of the Capernaum family. The raw material for extracting caper fruit is the fruit of the capernaum. Capernaum fruit extract contains a variety of functional components such as flavonoids and alkaloids, which have analgesic and anti-inflammatory effects. It can effectively act on opioid receptors and TRPV1 receptors, reduce the transmission of pain signals, and play a soothing role.

[0014] Bitter orange oil mainly contains chemical components such as dextrorotatory limonene, nerol, and citronellol, thus having a calming effect. At the same time, it also has a light, fresh, and sweet orange scent, which can provide users with a pleasant aroma and a relaxing feeling, increasing the sense of soothing.

[0015] The raw material for extracting Fucus vesiculosus is the dried algae of Fucus vesiculosus. Fucus vesiculosus extract is rich in fucoidan, glutamic acid, minerals, polyphenols and vitamins. Glutamic acid can increase skin smoothness, provide the skin with a sebum film, and help promote cell metabolism and regeneration of connective tissue. Minerals, polyphenols and vitamins can stimulate the body's natural healing process and have a certain repair effect.

[0016] The raw material for Artemisia capillaris extract is Artemisia capillaris, a plant belonging to the Artemisia genus of the Asteraceae family. Artemisia capillaris extract can increase the expression of filaggrin, promote cell damage repair, and thus gently repair the skin structure.

[0017] Furthermore, the solvent includes deionized water and anhydrous ethanol.

[0018] Furthermore, the deionized water and anhydrous ethanol are composed in a mass ratio of 4.5-5:1.

[0019] Furthermore, the preparation methods for the Polygala tenuifolia root extract, Caprifolia fruit extract, Fucus vesiculosus extract, and Artemisia capillaris extract are all as follows:

[0020] Mix the raw material with deionized water evenly, soak overnight at a controlled temperature, filter to obtain filtrate and residue, soak the residue repeatedly, combine the obtained filtrates to obtain soaking solution, spray dry the soaking solution to obtain the extract.

[0021] Furthermore, the amount of deionized water added is just enough to cover all the raw materials; the temperature controlled is 25-30℃; and the number of repeated soakings is 3-5 times.

[0022] A method for preparing a soothing and repairing composition specifically includes the following steps:

[0023] Bitter orange oil and hydrogenated rapeseed oil alcohol were stirred evenly under controlled temperature, and then anhydrous ethanol was added and stirred evenly to obtain mixture A. Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, β-glucan and deionized water were mixed evenly to obtain mixture B. Mixture A and mixture B were stirred evenly under controlled temperature to obtain the soothing and repairing composition.

[0024] Furthermore, the temperature for temperature control is 30-35℃.

[0025] Application of a soothing and repairing composition, wherein the soothing and repairing composition is added to a skin care product at a mass percentage of 2-2.5%.

[0026] Furthermore, the skincare products include, but are not limited to, toners, serums, and creams.

[0027] The beneficial effects of this invention are:

[0028] (1) This invention mainly focuses on adding raw materials with anti-inflammatory and sedative effects. It uses the combined action of Polygala tenuifolia root extract, Caprifolia fruit extract and bitter orange oil to avoid discomfort such as redness, itching and stinging of the skin in an inflammatory state. At the same time, the sedative ingredients can help the skin to soothe quickly, so that the soothed skin can better activate its self-repair mechanism. Therefore, the finished product of the composition has an excellent soothing effect.

[0029] (2) In this invention, β-glucan promotes and accelerates the migration of keratinocytes (HaCaT), Fucus vesiculosus extract assists in promoting cell metabolism and the regeneration of connective tissue, and Artemisia capillaris extract further promotes cell damage repair; therefore, the three can work synergistically to give the prepared composition excellent repair efficacy.

[0030] (3) The present invention also adds hydrogenated rapeseed oil alcohol to provide moisturizing and hydrating effects, increasing the skin's suppleness. In addition to its repairing effect, the β-glucan added in the present invention can also lock in skin moisture. Therefore, β-glucan can also assist hydrogenated rapeseed oil alcohol in making the finished product of the composition have a moisturizing and water-locking effect. Detailed Implementation

[0031] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0032] The bitter orange oil used in this embodiment of the invention was purchased from Jiangxi Chuanhui Herbal Biotechnology Co., Ltd.; β-glucan and hydrogenated rapeseed oil alcohol were purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.; the above will not be repeated hereafter.

[0033] Example 1

[0034] A soothing and repairing composition comprising the following ingredients in parts by weight:

[0035]

[0036]

[0037] The solvent is composed of deionized water and anhydrous ethanol in a mass ratio of 5:1;

[0038] The raw material for the extraction of the root extract of Polygala tenuifolia is its dried root bark; the raw material for the extraction of the fruit extract of Caprifolia arvense is the fruit of Caprifolia arvense; the raw material for the extraction of the extract of Fucus vesiculosus is its dried algae; and the raw material for the extraction of Artemisia capillaris is Artemisia capillaris.

[0039] The preparation methods for the Polygala tenuifolia root extract, Caprifolia fruit extract, Fucus vesiculosus extract, and Artemisia capillaris extract are all as follows:

[0040] The raw materials were mixed evenly with deionized water and soaked overnight at 25°C. After filtration, filtrate and residue were obtained. The residue was soaked 5 times, and the filtrates were combined to obtain soaking solution. The soaking solution was spray-dried to obtain Polygala tenuifolia root extract, Capsicum arvense fruit extract, Fucus vesiculosus extract and Artemisia capillaris extract, respectively.

[0041] The amount of deionized water added is just enough to cover all the raw materials;

[0042] A method for preparing a soothing and repairing composition specifically includes the following steps:

[0043] Bitter orange oil and hydrogenated rapeseed oil alcohol were stirred evenly at a controlled temperature of 32.5℃, and then anhydrous ethanol was added and stirred evenly to obtain mixture A. Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, β-glucan and deionized water were mixed evenly to obtain mixture B. Mixture A and mixture B were stirred evenly at a controlled temperature of 30℃ to obtain the soothing and repairing composition.

[0044] Example 2

[0045] A soothing and repairing composition comprising the following ingredients in parts by weight:

[0046]

[0047]

[0048] The solvent is composed of deionized water and anhydrous ethanol in a mass ratio of 4.75:1;

[0049] The raw material for the extraction of the root extract of Polygala tenuifolia is its dried root bark; the raw material for the extraction of the fruit extract of Caprifolia arvense is the fruit of Caprifolia arvense; the raw material for the extraction of the extract of Fucus vesiculosus is its dried algae; and the raw material for the extraction of Artemisia capillaris is Artemisia capillaris.

[0050] The preparation methods for the Polygala tenuifolia root extract, Caprifolia fruit extract, Fucus vesiculosus extract, and Artemisia capillaris extract are all as follows:

[0051] The raw materials were mixed evenly with deionized water and soaked overnight at 27.5℃. After filtration, filtrate and residue were obtained. The residue was soaked three times, and the filtrates were combined to obtain soaking solution. The soaking solution was spray-dried to obtain Polygala tenuifolia root extract, Capsicum serrata fruit extract, Fucus vesiculosus extract and Artemisia capillaris extract, respectively.

[0052] The amount of deionized water added is just enough to cover all the raw materials;

[0053] A method for preparing a soothing and repairing composition specifically includes the following steps:

[0054] Bitter orange oil and hydrogenated rapeseed oil alcohol were stirred evenly at a controlled temperature of 32.5℃, and then anhydrous ethanol was added and stirred evenly to obtain mixture A. Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, β-glucan and deionized water were mixed evenly to obtain mixture B. Mixture A and mixture B were stirred evenly at a controlled temperature of 30℃ to obtain the soothing and repairing composition.

[0055] Example 3

[0056] A soothing and repairing composition comprising the following ingredients in parts by weight:

[0057]

[0058]

[0059] The solvent is composed of deionized water and anhydrous ethanol in a mass ratio of 4.5:1;

[0060] The raw material for the extraction of the root extract of Polygala tenuifolia is its dried root bark; the raw material for the extraction of the fruit extract of Caprifolia arvense is the fruit of Caprifolia arvense; the raw material for the extraction of the extract of Fucus vesiculosus is its dried algae; and the raw material for the extraction of Artemisia capillaris is Artemisia capillaris.

[0061] The preparation methods for the Polygala tenuifolia root extract, Caprifolia fruit extract, Fucus vesiculosus extract, and Artemisia capillaris extract are all as follows:

[0062] The raw materials were mixed evenly with deionized water and soaked overnight at 30°C. After filtration, filtrate and residue were obtained. The residue was soaked 4 times, and the filtrates were combined to obtain soaking solution. The soaking solution was spray-dried to obtain Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract and Artemisia capillaris extract, respectively.

[0063] The amount of deionized water added is just enough to cover all the raw materials;

[0064] A method for preparing a soothing and repairing composition specifically includes the following steps:

[0065] Bitter orange oil and hydrogenated rapeseed oil alcohol were stirred evenly at 35°C, and then anhydrous ethanol was added and stirred evenly to obtain mixture A. Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, β-glucan and deionized water were mixed evenly to obtain mixture B. Mixture A and mixture B were stirred evenly at 32.5°C to obtain the soothing and repairing composition.

[0066] Comparative Example 1

[0067] Compared with Example 2, the difference is that no Polygala tenuifolia root extract was added in Comparative Example 1, while the extracts of Caprifolia fruit and bitter orange oil were both 1.05%; the other operating steps and parameters remained unchanged.

[0068] Comparative Example 2

[0069] Compared with Example 2, the difference is that no capernaum fruit extract was added in Comparative Example 2, while the amount of Polygala tenuifolia root extract was 1.5% and the amount of bitter orange oil was 0.6%; the other operating steps and parameters remained unchanged.

[0070] Comparative Example 3

[0071] Compared with Example 2, the difference is that bitter orange oil was not added in Comparative Example 3, while the amount of Polygala tenuifolia root extract was 1.5% and the amount of Caprifolia fruit extract was 0.6%; the other operating steps and parameters remained unchanged.

[0072] Comparative Example 4

[0073] Compared with Example 2, the difference is that no β-glucan was added in Comparative Example 4, while the content of Fucus vesiculosus extract was 0.2% and the content of Artemisia capillaris extract was 12%; the other operating steps and parameters remained unchanged.

[0074] Comparative Example 5

[0075] Compared with Example 2, the difference is that no Fucus vesiculosus extract was added in Comparative Example 5, while β-glucan was 0.2% and Artemisia capillaris extract was 12%; the other operating steps and parameters remained unchanged.

[0076] Comparative Example 6

[0077] Compared with Example 2, the difference is that Artemisia capillaris extract was not added in Comparative Example 6, while β-glucan was 6.1% and Fucus vesiculosus extract was 6.1%; the other operating steps and parameters remained unchanged.

[0078] Comparative Example 7

[0079] Compared with Example 2, the difference is that hydrogenated rapeseed oil alcohol was not added in Comparative Example 7, while β-glucan was 4.35%; the other operating steps and parameters remained unchanged.

[0080] Comparative Example 8

[0081] Compared with Example 2, the difference is that no β-glucan was added in Comparative Example 8, while the hydrogenated rapeseed oil alcohol content was 4.35%; the other operating steps and parameters remained unchanged.

[0082] Test Example 1

[0083] Soothing performance test:

[0084] Principle: Neutrophils in zebrafish embryos are highly similar to human neutrophils in morphology, biochemistry, and physiological function. Neutrophils are among the first white blood cells to appear at the site of injury or invasion when damage occurs or pathogens invade, and their main function is to clear infection or remove harmful substances. Copper sulfate was used to induce damage to neurothalamic cells in the lateral line region of zebrafish embryos, leading to neutrophil aggregation. The greater the damage to the neurothalamic cells, the more neutrophils aggregated. If the tested sample has a soothing effect, the number of neutrophils will decrease. The changes in the number of neutrophils in the lateral line region of the embryos in the tested sample group and the blank control group were compared, and the neutrophil inhibition rate of zebrafish embryos was calculated to evaluate the soothing efficacy of the tested sample.

[0085] (1) Test samples: The soothing and repairing compositions of Examples 1-3 and Comparative Examples 1-3 were prepared into a 2% solution by mass using fish embryo culture medium.

[0086] (2) Test Procedure (Test Sample Group):

[0087] (a) Select 6 fish embryos into a culture dish (6 fish embryos per test sample), add 5 mL of fish embryo culture medium (containing 0.16 mg / L copper sulfate pentahydrate) to the culture dish, and incubate at 28°C for 50 min.

[0088] (b) After fixing the test group fish embryos in paraformaldehyde for at least 1 hour, the fish embryos were treated with PBST solution, then stained with Sudan Black staining solution for 1 hour, and finally treated with bleaching solution to obtain fixed and stained fish embryos.

[0089] (c) Place the fish embryos on their side, observe and photograph them under a microscope, count the number of neutrophils in the three-quarters of the tail lateral line region from the anus of each fish embryo, take the average of the count results, retain one decimal place, and calculate the neutrophil aggregation inhibition rate.

[0090] (3) Test procedure (blank control group):

[0091] The test steps for the blank control group are similar to those for the test sample group, except that in step (a), the 5 mL of fish embryo culture medium (containing 0.16 mg / L copper sulfate pentahydrate) for the test sample is replaced with 5 mL of fish embryo culture medium (containing 0.16 mg / L copper sulfate pentahydrate), meaning that no test sample is added to the blank control group.

[0092] (4) Neutrophil aggregation inhibition rate (%) = (CT) / C*100%

[0093] C: Number of neutrophils in fish embryos of the blank control group; T: Number of neutrophils in fish embryos of the test sample group.

[0094] Evaluation criteria: The higher the neutrophil aggregation inhibition rate, the better the soothing effect of the test sample; the test results are shown in Table 1.

[0095] Table 1

[0096] neutrophil count Neutrophil aggregation inhibition rate (%) Blank control 25.5 —— Example 1 18.15 30.2 Example 2 17.99 30.8 Example 3 18.04 30.6 Comparative Example 1 20.20 22.3 Comparative Example 2 20.57 20.9 Comparative Example 3 19.58 24.7

[0097] As can be seen from Table 1, the soothing and repairing composition of the present invention has excellent soothing effects.

[0098] Test Example 2

[0099] Repair performance test:

[0100] Effect of the composition on Hacat cell scratch migration

[0101] Principle: Cell migration rate has a significant impact on skin repair and wound healing. During wound healing, cell migration is a crucial step in the process of epidermal cells covering the wound; therefore, the higher the cell migration rate, the faster the wound heals. Simultaneously, cell migration also participates in the repair process after skin injury. Impaired cell migration can lead to impaired skin repair, subsequently causing various skin diseases.

[0102] Test Method: HaCat cells were added to DMEM basal medium and incubated in a 37°C, 5% CO2 incubator for 24 hours. Cells were then gently scratched with a 200 μL pipette tip, the supernatant was discarded, and the cells were washed twice with culture medium before adding the sample. For the negative control group, 2 mL of DMEM basal medium was added to each well as the sample. For the positive control group, 2 mL of DMEM medium containing 5% fetal bovine serum was added to each well as the sample. The compositions prepared in Examples 1-3 and Comparative Examples 4-6 were diluted to a 2% concentration using culture medium, and 2 mL of the diluted sample was added to each well. The cells were then incubated in a 37°C, 5% CO2 incubator for 24 and 48 hours, respectively, and observed and photographed under a microscope. The scratch area in the images was scanned using ImageJ image analysis software, and the data were calculated and analyzed. The cell migration rate for each group was calculated using the following formula:

[0103] Mobility (%) - (T0 - Tx) / T0 × 100%;

[0104] Where: T0—initial measured migration area; Tx—measured migration area after 24 or 48 hours;

[0105] The test results are shown in Table 2.

[0106] Table 2

[0107] Cell migration rate (%) at 24 hours Cell migration rate (%) at 48 hours negative control 40.6 67.5 Positive control 62.7 84.2 Example 1 70.2 83.6 Example 2 70.5 83.9 Example 3 70.4 83.7 Comparative Example 4 61.4 75.4 Comparative Example 5 65.3 78.3 Comparative Example 6 62.5 76.1

[0108] As can be seen from Table 2, the finished product of the composition prepared by the present invention has excellent repair effects.

[0109] Test Example 3

[0110] Sixty volunteers aged 25-55 with dry skin problems were selected, including 30 men and 30 women, and randomly divided into 5 groups of 12 each. The volunteers used the essence prepared by the soothing and repairing composition in Examples 1-3 and Comparative Examples 7-8 (the amount of soothing and repairing composition added was 2%). The cream sample was applied to the face area of ​​the volunteers as the test area, once in the morning and once in the evening for 28 days.

[0111] Skin moisture content was measured using a Corneometer CM825 at 0 and 28 days after product use to assess skin moisture levels before and after cream use.

[0112] The results are shown in Table 3.

[0113] Table 3

[0114]

[0115]

[0116] As can be seen from Table 3, the finished product of the composition prepared by the present invention has the effect of replenishing and locking water.

[0117] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0118] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, they should all fall within the protection scope of the present invention.

Claims

1. A soothing and repairing composition, characterized in that, The composition comprises the following raw materials in parts by weight: Polygala tenuifolia root extract 0.001-1.5% Capernaum fruit extract 0.1-1% Fucus vesiculosus extract 0.1-5% Artemisia capillaris extract 0.1-25% Bitter orange oil 0.01-0.5% β-glucan 0.01-0.1% Hydrogenated rapeseed oil alcohol content: 0.01-4.25% Solvent balance.

2. The soothing and repairing composition according to claim 1, characterized in that, The solvents include deionized water and anhydrous ethanol.

3. The soothing and repairing composition according to claim 2, characterized in that, The deionized water and anhydrous ethanol are composed of a mass ratio of 4.5-5:

1.

4. The soothing and repairing composition according to claim 1, characterized in that, The preparation methods for the Polygala tenuifolia root extract, Caprifolia fruit extract, Fucus vesiculosus extract, and Artemisia capillaris extract are all as follows: Mix the raw material with deionized water evenly, soak overnight at a controlled temperature, filter to obtain filtrate and residue, soak the residue repeatedly, combine the obtained filtrates to obtain soaking solution, spray dry the soaking solution to obtain the extract.

5. The soothing and repairing composition according to claim 4, characterized in that, The amount of deionized water added is just enough to cover all the raw materials.

6. The soothing and repairing composition according to claim 4, characterized in that, The temperature for temperature control is 25-30℃; the number of repeated soakings is 3-5 times.

7. A method for preparing the soothing and repairing composition according to any one of claims 1-6, characterized in that, Specifically, the steps include the following: Bitter orange oil and hydrogenated rapeseed oil alcohol were stirred evenly under controlled temperature, and then anhydrous ethanol was added and stirred evenly to obtain mixture A. Polygala tenuifolia root extract, Capsella bursa-pastoris fruit extract, Fucus vesiculosus extract, Artemisia capillaris extract, β-glucan and deionized water were mixed evenly to obtain mixture B. Mixture A and mixture B were stirred evenly under controlled temperature to obtain the soothing and repairing composition.

8. The method for preparing the soothing and repairing composition according to claim 7, characterized in that, The temperature for temperature control is 30-35℃.

9. The use of a soothing and repairing composition as described in any one of claims 1-6 in the preparation of skincare products, characterized in that, The soothing and repairing composition is added to skincare products at a weight percentage of 2-2.5%.

10. The application of the soothing and repairing composition according to claim 9 in the preparation of skin care products, characterized in that, The skincare products include toner, serum, and face cream.

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