Toothpaste containing various traditional Chinese medicines and preparation method thereof

Abstract

The invention provides toothpaste containing various traditional Chinese medicines and a preparation method of the toothpaste, and the toothpaste containing the various traditional Chinese medicines comprises the following preparation raw materials in percentage by weight: 2-10% of a traditional Chinese medicine composition and the balance of a toothpaste matrix, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20 to 40 parts of radix arnebiae seu lithospermi, 5 to 10 parts of herba cistanche, 15 to 25 parts of radix curcumae from Sichuan of China, 5 to 10 parts of herba sedi, 15 to 30 parts of rhizoma bletillae and 10 to 20 parts of flos caryophylli The toothpaste containing various traditional Chinese medicines has remarkable effects in the aspects of diminishing inflammation, sterilizing, repairing gingival bleeding, enhancing immunity and the like, and can meet diversified oral health requirements of consumers.

Description

Technical Field

[0001] The invention belongs to the technical field of daily necessities, and particularly relates to a toothpaste containing multiple traditional Chinese medicines and a preparation method thereof. Background Art

[0002] Although traditional toothpaste can clean teeth and prevent tooth decay to a certain extent, it has limited effects in terms of anti-inflammatory and sterilization, repairing bleeding gums, and enhancing immunity. Chinese herbal medicine has unique advantages in the field of oral care due to its natural, mild and multi-effect characteristics. Although some Chinese herbal toothpastes are already on the market, most of them focus on a single effect, and the synergistic effect of Chinese herbal medicine compositions has not been fully studied, making it difficult to meet the diverse oral health needs of consumers.

[0003] In view of the deficiencies in the prior art, the present invention aims to provide a toothpaste containing multiple traditional Chinese medicines, which has significant effects in anti-inflammatory and sterilization, repairing gingival bleeding, enhancing immunity, etc., and can meet the diverse oral health needs of consumers. Summary of the invention

[0004] The purpose of the present invention is to provide a toothpaste containing multiple traditional Chinese medicines and a preparation method thereof. The toothpaste containing multiple traditional Chinese medicines has significant effects in anti-inflammatory and sterilization, repairing gingival bleeding, enhancing immunity, etc., and can meet the diverse oral health needs of consumers.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The toothpaste containing multiple traditional Chinese medicines comprises, by weight percentage, 2% to 10% of the traditional Chinese medicine composition as raw materials, and the rest is supplemented by the toothpaste base to 100%.

[0007] The raw materials for preparing the traditional Chinese medicine composition include, by weight, 20-40 parts of lithospermum officinale, 5-10 parts of cistanche deserticola, 15-25 parts of turmeric, 5-10 parts of scutellaria baicalensis, 15-30 parts of bletilla striata, and 10-20 parts of clove.

[0008] The inventor found that by selecting Radix Lithospermi, Herba Cistanches, Radix Curcumae, Herba Bletillae, and Cloves as the Chinese medicine composition of the present invention and extracting its active ingredients, the toothpaste prepared can have certain anti-inflammatory and bactericidal abilities, and can also repair bleeding gums and enhance oral immunity. The synergistic effects of the effective ingredients shikonin, alkaloids, glucomannan, eugenol, etc. in Radix Lithospermi, Herba Citrobii, Herba Bletillae, and Cloves not only have broad-spectrum antibacterial properties to a variety of oral pathogens, but also have the effects of clearing away heat and detoxicating, anti-inflammatory and analgesic, and have the effects of relieving symptoms such as oral ulcers and gingivitis. The compounding of Radix Lithospermi, Herba Bletillae, Herba Curcumae, and Herba Cistanches can not only rapidly control the symptoms of bleeding gums, alleviate inflammatory reactions, but also promote new skin regeneration, accelerate the repair and regeneration process of gingival tissue, and on the other hand, the compounding of Herba Curcumae and Herba Cistanches can accelerate the repair process and promote the self-repairing ability of gingival tissue by improving the blood circulation in the oral cavity and enhancing the immunity of the body. However, if you want the toothpaste to have the best effect, you need to extract as many effective ingredients from traditional Chinese medicine as possible through specific methods.

[0009] The preparation method of the Chinese medicine composition comprises the following steps:

[0010] Prepare the extract of Lithospermum officinale and Herba Crepisae together to obtain the extract of Lithospermum officinale and Herba Crepisae;

[0011] Cistanche deserticola, Bletilla striata and clove are prepared together to obtain an extract of Cistanche deserticola, Bletilla striata and clove;

[0012] The turmeric extract is prepared separately from the turmeric extract;

[0013] Then the extracts of Lithospermum officinale and Herba Creepersii, the extracts of Cistanche deserticola, Bletilla striata and Clove, and Curcuma acuminata extract are mixed to obtain the Chinese medicine composition.

[0014] The preparation method of the extract of lithospermum and creeping vine grass comprises the following steps: drying lithospermum and creeping vine grass and then crushing them, passing through a 30-50 mesh sieve, adding an ethanol aqueous solution with a volume fraction of 50%-60%, stirring evenly and adjusting the pH to 4-5, adding an enzyme, ultrasonically treating and then centrifuging, vacuum filtering to obtain a filtrate, repeating the above operation on the filter residue, combining the filtrate, and decompressing and concentrating the filtrate to dryness to obtain the extract of lithospermum and creeping vine grass.

[0015] Preferably, the solid-liquid ratio of the total mass of the lithospermum and the spatholobi to the ethanol aqueous solution is 1 g: 30-50 mL.

[0016] Preferably, the added amount of the enzyme is 0.2% to 0.5% of the total mass of Lithospermum officinale and Scutellaria baicalensis.

[0017] Preferably, the enzymes are cellulase and pectinase.

[0018] Preferably, the mass ratio of the cellulase to the pectinase is (2-4):1; more preferably, it is 3:1.

[0019] Preferably, the enzymatic activity of the cellulase is ≥10000u / g.

[0020] In some preferred embodiments, the cellulase is purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0021] Preferably, the enzymatic activity of the pectinase is ≥25000u / g.

[0022] In some preferred embodiments, the pectinase is purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0023] Preferably, the specific conditions of the ultrasonic treatment are: ultrasonic power of 500-600 W, ultrasonic time of 40-60 min, ultrasonic temperature of 35-50° C., and 4 seconds interval between each ultrasonic treatment.

[0024] The inventors found that the use of specific enzymes and ultrasonic conditions to treat lithospermum and scutellaria baicalensis can improve the antibacterial and anti-inflammatory properties of toothpaste and relieve gum bleeding. This may be because the effective ingredients in lithospermum and scutellaria baicalensis, such as shikonin and total flavonoids, are mostly present in the cell wall. Ultrasonic treatment combined with enzymes can not only accelerate the rupture of plant cell walls, reduce the transfer resistance of active ingredients, promote the extraction and separation of active ingredients, but also avoid the high temperature of water extraction from destroying active ingredients. Specific ultrasonic conditions and specific enzymes work synergistically, on the one hand, increase the catalytic activity of pectinase, help destroy plant cell walls, and reduce the mass transfer resistance of shikonin and total flavonoids; on the other hand, the solubility and diffusion coefficient of shikonin and total flavonoids are improved, which is conducive to the extraction of shikonin and total flavonoids. By leaving a gap of 4s after each ultrasonic 2s, the treatment liquid can be provided with time for heat dissipation, avoiding the occurrence of overheating, and affecting the stability and extraction efficiency of shikonin and total flavonoids. In the actual preparation process, the conditions need to be controlled. On the one hand, too high a temperature will affect the enzyme activity, and on the other hand, it will also destroy the structure of shikonin and total flavonoids, thereby reducing the extraction rate of shikonin and total flavonoids. If the amount of enzyme added is too high, the cell wall will be broken more thoroughly. At this time, the solvent will dissolve too many impurities, which will reduce the solubility of shikonin and total flavonoids, which is not conducive to the extraction of shikonin and total flavonoids. High concentrations of ethanol will dissolve alcohol-soluble impurities and pigments, thereby reducing the solubility of shikonin and total flavonoids, resulting in a decrease in the extraction rate and affecting the beneficial effects of toothpaste.

[0025] The preparation method of the turmeric extract comprises the following steps:

[0026] A1. Take Sichuan turmeric and dry it, grind it through a 30-50 mesh sieve, and reflux extract it twice with a 65%-75% ethanol aqueous solution, each time for 1-2 hours, filter it, and combine the two extracts to obtain a Sichuan turmeric extract;

[0027] A2. The extracted turmeric extract was adjusted to pH 1-2 with 6 mol / L hydrochloric acid, kept at 75-85°C for 0.2-3 h, and then allowed to stand at room temperature for 10-14 h. The precipitate was washed with water, 50% ethanol aqueous solution, and 95% ethanol aqueous solution in sequence until neutral. The ethanol aqueous solution was evaporated and dried at 55-65°C to obtain a crude turmeric extract.

[0028] A3. Dissolve the crude extract of Sichuan turmeric in water, pass it through a macroporous resin at a flow rate of 2.5-3.5 BV / h, and elute it with 3-5 BV of water and 2-4 BV of 50% ethanol aqueous solution in sequence. Collect the eluate, concentrate and dry it under reduced pressure to obtain the Sichuan turmeric extract.

[0029] Preferably, the solid-to-liquid ratio of the crude extract of Curcuma azalea and water is 1 g:10 mL.

[0030] Preferably, the macroporous resin is a non-polar macroporous resin with an average pore size of 8.5 to 9 nm, a pore volume of 1.35 to 1.65 mL / g, and a specific surface area of ​​≥600 m 2 / g.

[0031] In some preferred embodiments, the macroporous resin is purchased from Shandong Shoucheng Chemical Co., Ltd., HPD100.

[0032] When preparing the crude extract of Curcuma acuminata, the pH of the Curcuma acuminata extract is adjusted to be acidic, especially when the pH is 1, the content of effective substances in the extract can be increased. This is because the terpene compounds that are beneficial to toothpaste are all weakly acidic, and their dissociation can be inhibited in an acidic environment, so that they are in a molecular form in the solvent and are more easily freed. By selecting a specific resin to enrich and purify the crude extract of Curcuma acuminata, and eluting under specific conditions, the content of effective substances in the Curcuma acuminata extract can be further increased. This is because the resin selected by the present invention is a non-polar macroporous adsorption resin, and this non-polar property makes it have a good affinity for non-polar or weakly polar terpene compounds. The non-polar part in the molecular structure of terpene compounds can interact with the non-polar surface of the resin, thereby achieving effective adsorption. In addition, the resin selected by the present invention has a porous structure, and these pores provide abundant adsorption sites for terpene compound molecules. At the same time, the ethanol concentration should not be too high. The problems such as the destruction of the terpene compound structure or the poor elution effect of low-concentration ethanol that may be caused by high concentrations of ethanol affect the content of terpene compounds in the product. Through the specific extraction and purification steps of the present invention, a large amount of impurities in Sichuan Curcuma are removed and effective components are retained, thereby improving the purity and stability of the extract.

[0033] The preparation method of the extract of Cistanche deserticola, Bletilla striata and clove comprises the following steps: drying Cistanche deserticola, Bletilla striata and clove, crushing through a 30-50 mesh sieve, adding water and stirring evenly, adding fermentation bacteria, sealing and performing anaerobic fermentation at a fermentation temperature of 25-35°C, adding enzyme preparation after fermentation for 3 days, sealing and performing anaerobic fermentation at 35-45°C for 4 days to obtain the extract.

[0034] Preferably, the amount of water added is 45% to 55% of the total mass of Cistanche deserticola, Bletilla striata and Clove.

[0035] Preferably, the added amount of the fermentation bacteria is 4% to 8% of the total mass of Cistanche deserticola, Bletilla striata and Clove.

[0036] Preferably, the fermentation bacteria is a mixture of Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus rhamnosus.

[0037] Preferably, the mass ratio of Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus rhamnosus is (1-3):(2-4):1; more preferably, it is 2:3:1.

[0038] Preferably, the number of active yeast cells of the brewer's yeast is ≥ 15 billion / g.

[0039] In some preferred embodiments, the brewer's yeast is purchased from Shandong Yingfeida Biotechnology Co., Ltd.

[0040] Preferably, the content of active lactic acid bacteria in the Lactobacillus plantarum and Lactobacillus rhamnosus are both ≥ 5 billion cfu / g.

[0041] In some preferred embodiments, the Lactobacillus plantarum and Lactobacillus rhamnosus are both purchased from Xi'an Xihai Biotechnology Co., Ltd.

[0042] Preferably, the enzyme preparation is cellulase, hemicellulase and tannase.

[0043] Preferably, the mass ratio of the cellulase, hemicellulase and tannase is 1:(0.5-1.5):(1.5-2.5); further preferably, it is 1:1:2.

[0044] Preferably, the enzymatic activity of the cellulase is ≥10000u / g.

[0045] In some preferred embodiments, the cellulase is purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0046] Preferably, the enzymatic activity of the hemicellulase is ≥45000u / g.

[0047] In some preferred embodiments, the cellulase and hemicellulase are purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0048] Preferably, the tannase activity is ≥450u / g

[0049] In some preferred embodiments, the tannase is purchased from Shanghai Huashang Xiangyang Biotechnology Co., Ltd.

[0050] Preferably, the added amount of the enzyme preparation is 1% to 3% of the total mass of Cistanche deserticola, Bletilla striata and Clove.

[0051] The inventor found that after using specific fermentation bacteria to ferment Cistanche deserticola, Bletilla striata, and cloves, adding a specific enzyme preparation for anaerobic treatment can greatly improve the effective ingredient content of Cistanche deserticola saponin, Bletilla striata polysaccharide, clove oil and eugenol in the Chinese medicine composition, thereby improving the performance of toothpaste. This may be because on the one hand, three kinds of fermentation bacteria synergize, produce different metabolites during the fermentation process, there is synergy between these metabolites, and act on the effective ingredient in the raw material together, not only can destroy the cell wall structure, make other fermentation bacteria or enzymes more easily contact the effective ingredient in the cell, also have antibacterial effect, protect the fermentation system from the pollution of harmful microorganisms. On the other hand, three kinds of enzyme compounding can not only destroy Cistanche deserticola, Bletilla striata, and clove cell wall structure more comprehensively, release the effective ingredient in the cell, and can also significantly improve the extraction efficiency of the extract, make the effective ingredient in the cell more easily dissolved, remove the bad flavor components in the extract, improve the purity and quality of the extract, thereby avoiding the peculiar smell of toothpaste, affecting use. In addition, there is also a synergistic effect between fermentation bacteria and enzyme preparations. Fermentation bacteria will produce various metabolites during the fermentation process, such as organic acids, alcohols, amino acids, etc. These metabolites can adjust the pH value, osmotic pressure and other environmental parameters of the fermentation system, and provide a more suitable working environment for enzyme preparations. The addition of enzyme preparations can decompose the complex components in the raw materials and generate small molecules that are easily used by fermentation bacteria, thereby promoting the growth and metabolic activities of fermentation bacteria. This mutually reinforcing relationship makes the fermentation process more efficient and stable. The toothpaste matrix, by weight, includes 15 to 30 parts of abrasives, 1 to 3 parts of thickeners, 5 to 10 parts of moisturizers, 10 to 20 parts of foaming agents, and 5 to 10 parts of deionized water.

[0052] Preferably, the friction agent is silicon dioxide.

[0053] Preferably, the particle size of the silicon dioxide is ≤100 nm.

[0054] In some preferred embodiments, the silicon dioxide is purchased from Shijiazhuang Borui Building Materials Co., Ltd.

[0055] Preferably, the thickener is xanthan gum.

[0056] Preferably, the shear performance value of the xanthan gum is ≥6.5, the pH value of a 1% xanthan gum aqueous solution is 6-8, and the viscosity of the xanthan gum at a mass fraction of 1% in a 1% KCl solution is 1200-1500 mPa·s; further preferably, the shear performance value of the xanthan gum is 7.6, the pH value of a 1% xanthan gum aqueous solution is 7.2, and the viscosity of the xanthan gum at a mass fraction of 1% in a 1% KCl solution is 1370 mPa·s.

[0057] In some preferred embodiments, the xanthan gum is purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0058] Preferably, the humectant is glycerin.

[0059] Preferably, the foaming agent is sodium laurate sarcosinate.

[0060] The preparation method of the toothpaste containing multiple traditional Chinese medicines comprises the following steps: adding the traditional Chinese medicine composition into the toothpaste matrix, heating to 70-80° C., and stirring for 1-2 hours under vacuum conditions to obtain the toothpaste.

[0061] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0062] 1. The present invention provides a toothpaste containing multiple traditional Chinese medicines and a preparation method thereof. The toothpaste containing multiple traditional Chinese medicines has significant effects in anti-inflammatory and sterilization, repairing gingival bleeding, enhancing immunity, etc., and can meet the diverse oral health needs of consumers.

[0063] 2. The present invention selects Lithospermum officinale, Cistanche deserticola, Curcuma aromatica, Herba Creepersii, Bletilla striata, and Clove as the Chinese medicine composition of the present invention and extracts the active ingredients thereof, so that the prepared toothpaste has certain anti-inflammatory and bactericidal abilities, and can also repair gingival bleeding and enhance oral immunity.

[0064] 3. The present invention uses specific enzymes and ultrasonic conditions to treat Lithospermum officinale and Herba Lysimachiae, which can improve the antibacterial and anti-inflammatory and relieving gum bleeding capabilities of the toothpaste.

[0065] 4. The present invention can further increase the content of effective substances in the turmeric extract by selecting a specific resin to enrich and purify the turmeric crude extract and eluting it under specific conditions.

[0066] 5. The present invention uses specific fermentation bacteria to ferment Cistanche deserticola, Bletilla striata and clove, and then adds specific enzyme preparations for anaerobic treatment, which can greatly increase the content of effective ingredients such as Cistanche deserticola saponin, Bletilla striata polysaccharide, clove oil and eugenol in the traditional Chinese medicine composition, thereby improving the performance of the toothpaste. DETAILED DESCRIPTION

[0067] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0068] The raw materials used in the present invention are all commercially available, specifically:

[0069] The enzymatic activity of cellulase is about 11000u / g, the enzymatic activity of pectinase is about 30000u / g, and the enzymatic activity of hemicellulase is about 50000u / g, all of which were purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0070] The enzyme activity of tanninase was about 500u / g and was purchased from Shanghai Huashang Xiangyang Biotechnology Co., Ltd.

[0071] The macroporous resin is a non-polar macroporous resin with an average pore size of 8.5-9 nm, a pore volume of 1.35-1.65 mL / g, and a specific surface area of ​​≥600 m 2 / g, purchased from Shandong Shoucheng Chemical Co., Ltd., HPD100.

[0072] The number of active yeast cells of Saccharomyces cerevisiae was about 15 billion / g, which was purchased from Shandong Infida Biotechnology Co., Ltd.

[0073] The active lactic acid bacteria content in Lactobacillus plantarum and Lactobacillus rhamnosus was about 5 billion cfu / g, and both were purchased from Xi'an Xihai Biotechnology Co., Ltd.

[0074] The particle size of silicon dioxide was ≤100 nm and was purchased from Shijiazhuang Borui Building Materials Co., Ltd.

[0075] The shear performance value of xanthan gum is 7.6, the pH value of 1% xanthan gum aqueous solution is 7.2, and the viscosity of xanthan gum at 1% mass fraction in 1% KCl solution is 1370 mPa·s. It was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0076] Example 1

[0077] This embodiment provides a toothpaste containing multiple traditional Chinese medicines. The raw materials for its preparation include 5% of the traditional Chinese medicine composition and the rest of the toothpaste base is supplemented to 100% by weight.

[0078] The raw materials for preparing the traditional Chinese medicine composition include, by weight, 30 parts of lithospermum officinale, 8 parts of cistanche deserticola, 20 parts of turmeric, 8 parts of scutellaria baicalensis, 25 parts of bletilla striata and 15 parts of clove.

[0079] The preparation method of the traditional Chinese medicine composition comprises the following steps: preparing lithospermum and scutellaria baicalensis together to obtain extracts of lithospermum and scutellaria baicalensis; preparing extracts of Cistanche deserticola, Bletilla striata and Clove together to obtain extracts of Cistanche deserticola, Bletilla striata and Clove; preparing Curcuma acuminata alone to obtain Curcuma acuminata extract; and then mixing the extracts of lithospermum and scutellaria baicalensis, the extracts of Cistanche deserticola, Bletilla striata and Clove, and the Curcuma acuminata extract to obtain the traditional Chinese medicine composition.

[0080] The preparation method of the extract of lithospermum and creeping vine grass comprises the following steps: drying lithospermum and creeping vine grass and then crushing them, passing through a 40-mesh sieve, adding an ethanol aqueous solution with a volume fraction of 55%, stirring evenly and adjusting the pH to 4.5, adding an enzyme, ultrasonically treating and then centrifuging, vacuum filtering to obtain a filtrate, repeating the above operation on the filter residue, combining the filtrate, and decompressing and concentrating the filtrate to dryness to obtain the extract of lithospermum and creeping vine grass.

[0081] The solid-liquid ratio of the total mass of the lithospermum and the scutellaria baicalensis to the ethanol aqueous solution is 1 g:40 mL.

[0082] The added amount of the enzyme is 0.4% of the total weight of Lithospermum officinale and Scutellaria baicalensis.

[0083] The enzymes are cellulase and pectinase.

[0084] The mass ratio of the cellulase to the pectinase is 3:1.

[0085] The specific conditions of the ultrasonic treatment are as follows: ultrasonic power of 550 W, ultrasonic time of 50 min, ultrasonic temperature of 40° C., and a 4-second interval between each 2-second ultrasonic treatment.

[0086] The preparation method of the turmeric extract comprises the following steps:

[0087] A1. Take Sichuan turmeric and dry it, grind it through a 40-mesh sieve, and reflux extract it with 70% ethanol aqueous solution twice, each time for 1.5 hours, filter it, and combine the two extracts to obtain Sichuan turmeric extract;

[0088] A2. The extracted turmeric extract was adjusted to pH 1 with 6 mol / L hydrochloric acid, kept at 80°C for 1 hour, and then allowed to stand at room temperature for 12 hours. The precipitate was washed with water, 50% ethanol aqueous solution, and 95% ethanol aqueous solution in sequence until neutral. The ethanol aqueous solution was evaporated and dried at 60°C to obtain a crude turmeric extract.

[0089] A3. Dissolve the crude extract of Curcuma australis in water, pass it through a macroporous resin at a flow rate of 3BV / h, and elute it with 4BV of water and 3BV of 50% ethanol aqueous solution in sequence. Collect the eluate, concentrate and dry it under reduced pressure to obtain the Curcuma australis extract.

[0090] The solid-to-liquid ratio of the crude extract of Curcuma australis and water is 1 g:10 mL.

[0091] The preparation method of the extract of Cistanche deserticola, Bletilla striata and clove comprises the following steps: drying Cistanche deserticola, Bletilla striata and clove, crushing through a 40-mesh sieve, adding water and stirring evenly, adding fermentation bacteria, sealing and performing anaerobic fermentation at a fermentation temperature of 30°C, adding enzyme preparation after fermentation for 3 days, and sealing and performing anaerobic fermentation at 30°C for 4 days to obtain the extract.

[0092] The added amount of water is 50% of the total weight of Cistanche deserticola, Bletilla striata and Clove.

[0093] The added amount of the fermentation bacteria is 6% of the total weight of Cistanche deserticola, Bletilla striata and Clove.

[0094] The fermentation bacteria is a mixture of saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus.

[0095] The mass ratio of the brewer's yeast, Lactobacillus plantarum and Lactobacillus rhamnosus is 2:3:1.

[0096] The enzyme preparations are cellulase, hemicellulase and tannase.

[0097] The mass ratio of the cellulase, the hemicellulase and the tannase is 1:1:2.

[0098] The added amount of the enzyme preparation is 2% of the total mass of Cistanche deserticola, Bletilla striata and Clove.

[0099] The toothpaste matrix comprises, by weight, 25 parts of abrasive, 2 parts of thickener, 8 parts of moisturizer, 15 parts of foaming agent and 8 parts of deionized water.

[0100] The friction agent is silicon dioxide.

[0101] The thickener is xanthan gum.

[0102] The humectant is glycerin.

[0103] The foaming agent is sodium laurate sarcosinate.

[0104] The preparation method of the toothpaste containing multiple traditional Chinese medicines comprises the following steps: adding the traditional Chinese medicine composition into the toothpaste matrix, heating to 75° C., and stirring for 1.5 hours under vacuum conditions to obtain the toothpaste.

[0105] Example 2

[0106] The difference between this embodiment and embodiment 1 is that the raw materials for preparing the Chinese medicine composition, measured by weight, include 40 parts of lithospermum officinale, 10 parts of Cistanche deserticola, 25 parts of Curcuma aromatica, 10 parts of Creeping Peony vine, 20 parts of Bletilla striata, and 10 parts of Clove.

[0107] Example 3

[0108] The difference between this embodiment and embodiment 1 is that the raw materials for preparing the Chinese medicine composition, measured by weight, include 20 parts of lithospermum officinale, 5 parts of Cistanche deserticola, 15 parts of Curcuma aromatica, 5 parts of Creeping Peony, 15 parts of Bletilla striata, and 20 parts of cloves.

[0109] Comparative Example 1

[0110] The difference between this comparative example and Example 1 is that the raw materials for preparing the Chinese medicine composition, by weight, are 8 parts of Cistanche deserticola, 20 parts of Curcuma aromatica, 8 parts of Herba Creepersii, 25 parts of Bletilla striata, and 15 parts of Clove.

[0111] Comparative Example 2

[0112] The difference between this comparative example and Example 1 is that the raw materials for preparing the Chinese medicine composition, measured by weight, include 30 parts of Lithospermum officinale, 8 parts of Cistanche deserticola, 8 parts of Herba Crepisae, 25 parts of Bletilla striata, and 15 parts of Clove.

[0113] Comparative Example 3

[0114] The difference between this comparative example and Example 1 is that the added amount of the enzyme is 1% of the total mass of Lithospermum officinale and Scutellaria baicalensis.

[0115] Comparative Example 4

[0116] The difference between this comparative example and Example 1 is that the enzyme is cellulase.

[0117] Comparative Example 5

[0118] The difference between this comparative example and Example 1 is as follows: A2. The pH of the extracted turmeric extract is adjusted to 4 with 6 mol / L hydrochloric acid, and after being kept at 80°C for 1 hour, it is allowed to stand at room temperature for 12 hours, centrifuged, and the precipitate is washed with water, 50% ethanol aqueous solution, and 95% ethanol aqueous solution in sequence until neutral, the ethanol aqueous solution is evaporated, and the extract is dried at 60°C to obtain a crude turmeric extract.

[0119] Comparative Example 6

[0120] The difference between this comparative example and Example 1 is as follows: A3, the crude extract of Sichuan turmeric is dissolved in water, passed through a macroporous resin at a flow rate of 3BV / h, eluted with 4BV of water and 3BV of 90% ethanol aqueous solution in sequence, the eluate is collected, concentrated and dried under reduced pressure, and Sichuan turmeric extract is obtained.

[0121] The difference between this comparative example and Example 1 is that the fermentation bacteria is a mixture of saccharomyces cerevisiae and Lactobacillus plantarum.

[0122] The mass ratio of brewer's yeast to lactobacillus plantarum is 2:3.

[0123] Comparative Example 8

[0124] The difference between this comparative example and Example 1 is that the enzyme preparation is cellulase and tannase.

[0125] The mass ratio of the cellulase to the tannase is 1:2.

[0126] Comparative Example 9

[0127] The difference between this comparative example and Example 1 is that the preparation method of the extract of Cistanche deserticola, Bletilla striata and clove comprises the following steps: Cistanche deserticola, Bletilla striata and clove are dried, crushed through a 40-mesh sieve, added with water and stirred evenly, and then fermented with fermentation bacteria, sealed for anaerobic fermentation, the fermentation temperature is 30°C, and the fermentation is carried out for 7 days to obtain the product.

[0128] Performance Testing

[0129] Referring to the method in WS / T 326.3-2010 "Toothpaste Efficacy Evaluation Part 3: Inhibition of Dental Plaque and (or) Relief of Gingival Inflammation", people with BOP (i.e., the percentage of sites with bleeding after probing to the total sites in the whole mouth) ≥ 50% were selected, and 3 people were selected for each toothpaste sample. The initial BOP was recorded as CI. The toothpaste of the present invention was used in the morning and evening, and a light diet was eaten at the same time. After one week of use, the BOP was measured and recorded as C2. The BOP change rate was (C1-C2) / C1×100%. The antibacterial rate of the Chinese medicine composition against Staphylococcus aureus was measured by referring to the method in QB / T 2738-2012 "Evaluation Method for Antibacterial and Antibacterial Effect of Daily Chemical Products". The results are shown in Table 1.

[0130] Table 1 Measurement results

[0131]

[0132]

[0133] According to statistics, the toothpaste containing multiple Chinese medicines prepared by embodiments 1 to 3 of the present invention has good effect in reducing gingivitis, and has high antibacterial rate. Comparative Example 1 does not add Lithospermum officinale, Comparative Example 2 does not add Curcuma australis, Comparative Example 3 enzyme addition is too high, Comparative Example 4 is continuously ultrasonic, Comparative Example 5 pH is relatively low, Comparative Example 6 eluted with ethanol aqueous solution concentration is too high, Comparative Example 7 does not add Lactobacillus rhamnosus, Comparative Example 8 does not add hemicellulase, Comparative Example 9 does not add enzyme preparation, and the prepared toothpaste containing multiple Chinese medicines has poor anti-inflammatory effect and antibacterial effect. Therefore, the toothpaste containing multiple Chinese medicines prepared by the method described in the present application has significant effects in anti-inflammatory and sterilization, repairing gingival bleeding, enhancing immunity, etc., and can meet the diverse oral health needs of consumers.

[0134] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A preparation method of a toothpaste containing multiple Chinese medicines, characterized in that: The toothpaste comprises, by weight percentage, 2% to 10% of the raw materials of the Chinese medicine composition, and the toothpaste base supplements the balance to 100%; The raw materials for preparing the traditional Chinese medicine composition include, by weight, 20-40 parts of lithospermum officinale, 5-10 parts of cistanche deserticola, 15-25 parts of turmeric, 5-10 parts of scutellaria baicalensis, 15-30 parts of bletilla striata, and 10-20 parts of cloves; The preparation method of the Chinese medicine composition comprises the following steps: Prepare the extract of Lithospermum officinale and Herba Crepisae together to obtain the extract of Lithospermum officinale and Herba Crepisae; Cistanche deserticola, Bletilla striata and clove are prepared together to obtain an extract of Cistanche deserticola, Bletilla striata and clove; The turmeric extract is prepared separately from the turmeric extract; Then, the extracts of Lithospermum officinale and Herba Creepersii, the extracts of Cistanche deserticola, Bletilla striata and Clove, and the extract of Curcuma australis are mixed to obtain a Chinese medicine composition; The preparation method of the extract of lithospermum and creeping vine grass comprises: drying lithospermum and creeping vine grass and then crushing them, passing through a 30-50 mesh sieve, adding an ethanol aqueous solution with a volume fraction of 50%-60%, stirring evenly and adjusting the pH to 4-5, adding an enzyme, ultrasonically treating and then centrifuging, vacuum filtering to obtain a filtrate, repeating the above operation on the filter residue, combining the filtrate, and decompressing and concentrating the filtrate to dryness to obtain the extract of lithospermum and creeping vine grass.

2. The preparation of the toothpaste containing multiple Chinese medicines according to claim 1, characterized in that: The preparation method of the turmeric extract comprises the following steps: A1. Take Sichuan turmeric, dry it, crush it, pass it through a 30-50 mesh sieve, and reflux extract it twice with a 65%-75% ethanol aqueous solution, each time for 1-2 hours, filter it, and combine the two extracts to obtain a Sichuan turmeric extract; A2. The extracted turmeric extract was adjusted to pH 1-2 with 6 mol / L hydrochloric acid, kept at 75-85°C for 0.2-3 h, and then allowed to stand at room temperature for 10-14 h. The precipitate was washed with water, 50% ethanol aqueous solution, and 95% ethanol aqueous solution in sequence until neutral. The ethanol was evaporated and dried at 55-65°C to obtain a crude turmeric extract. A3. Dissolve the crude extract of Sichuan turmeric in water, pass it through a macroporous resin at a flow rate of 2.5-3.5 BV / h, and elute it with 3-5 BV of water and 2-4 BV of 50% ethanol aqueous solution in sequence. Collect the eluate, concentrate and dry it under reduced pressure to obtain the Sichuan turmeric extract.

3. The preparation of toothpaste containing multiple Chinese medicines according to claim 1, characterized in that: The preparation method of the extract of Cistanche deserticola, Bletilla striata and clove comprises the following steps: drying Cistanche deserticola, Bletilla striata and clove, crushing through a 30-50 mesh sieve, adding water and stirring evenly, adding fermentation bacteria, sealing and performing anaerobic fermentation at a fermentation temperature of 25-35°C, adding enzyme preparation after fermentation for 3 days, sealing and performing anaerobic fermentation at 35-45°C for 4 days to obtain the extract.

4. The preparation of toothpaste containing multiple Chinese medicines according to claim 1, characterized in that: The specific conditions of the ultrasonic treatment are as follows: ultrasonic power of 500-600 W, ultrasonic time of 40-60 min, ultrasonic temperature of 35-50° C., and ultrasonic treatment of 2 s with a rest of 4 s.

5. The preparation of the toothpaste containing multiple Chinese medicines according to claim 3, characterized in that: The fermentation bacteria is a mixture of saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus.

6. The preparation of toothpaste containing multiple Chinese medicines according to claim 5, characterized in that: The mass ratio of the brewer's yeast, Lactobacillus plantarum and Lactobacillus rhamnosus is (1-3):(2-4):

1.

7. The preparation of toothpaste containing multiple Chinese medicines according to claim 3, characterized in that: The enzyme preparations are cellulase, hemicellulase and tannase.

8. The preparation of the toothpaste containing multiple Chinese medicines according to claim 7, characterized in that: The mass ratio of the cellulase, hemicellulase and tannase is 1:(0.5-1.5):(1.5-2.5).

9. The preparation of toothpaste containing multiple Chinese medicines according to claim 8, characterized in that: The friction agent is silicon dioxide; the particle size of the silicon dioxide is ≤100nm.

10. A method for preparing a toothpaste containing multiple Chinese medicines according to any one of claims 1 to 9, characterized in that The method comprises the following steps: adding the traditional Chinese medicine composition into a toothpaste matrix, heating the mixture to 70-80° C., and stirring the mixture for 1-2 hours under vacuum conditions to obtain the toothpaste.

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