Fresh water chlorella carbon sink amount calculation method and system

Abstract

The invention discloses a fresh water chlorella genus carbon sink amount calculation method and system, and belongs to the technical field of carbon sink measurement. Firstly, the OD680 value and the biomass of the freshwater chlorella are collected to obtain a correlation model, then the actual carbon sink amount is obtained by combining a carbon dioxide absorption amount formula, then a predicted carbon sink amount calculation formula is constructed, and the predicted carbon sink amount can be output by inputting the real-time OD680 value. According to the method, the carbon sink amount is estimated in real time by dynamically monitoring the OD680 value, the repeated determination of biomass and carbon content is avoided, the operation process is simplified, the cost is reduced, the carbon sink potential of the freshwater chlorella can be quickly and accurately evaluated, the optimal harvesting time is helped to be determined, and the maximization of the cost benefit and the carbon sink potential is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of carbon sink measurement, and more particularly to a method and system for calculating the carbon sink of freshwater Chlorella. Background Art

[0002] Algae carbon sink refers to the absorption of carbon dioxide from the atmosphere by algae and its fixation in organisms or ecosystems. Freshwater Chlorella is a super oxygen-releasing organism that exceeds any terrestrial plant in the world. Because freshwater Chlorella can convert and utilize a large amount of carbon dioxide (CO 2 ), and store it as biomass, so it is an excellent "carbon sink" organism. Through large-scale, efficient, intensive and clean production of freshwater Chlorella, atmospheric CO can be effectively absorbed and utilized. 2 , mitigating climate change. In the process of artificially cultivating freshwater Chlorella, it is necessary to determine the best harvest time based on the carbon sink absorption of the algae during the production process, so as to obtain the maximum carbon sink potential.

[0003] The amount of algae carbon sink absorption is generally calculated based on the algae biomass and carbon content. Each time the carbon sink absorption is determined, it is necessary to measure the biomass and carbon content of the dry matter algae, resulting in huge workload and economic costs.

[0004] Therefore, how to propose a method and system for calculating the carbon sink of freshwater Chlorella to quickly estimate the carbon sink of freshwater Chlorella is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention provides a method and system for calculating the carbon sink amount of freshwater Chlorella, which estimates the carbon sink absorption amount of freshwater Chlorella in real time by dynamically monitoring OD680, so as to solve the problems of complex operation, high cost, difficulty in rapid and accurate monitoring, etc. of the existing estimation methods, and realize a simple, efficient and accurate evaluation of the carbon sink potential of freshwater Chlorella.

[0006] In order to achieve the above object, the present invention adopts the following technical solution: In one aspect, the present invention provides a method for calculating the carbon sink of freshwater Chlorella, comprising the following steps: Collect the OD680 value and corresponding biomass of freshwater Chlorella, and obtain the correlation model between biomass and OD680 value; The actual carbon sink amount is obtained based on the correlation model and the calculation formula of the carbon dioxide absorption amount; the relationship between the actual carbon sink amount and the OD680 value is analyzed, and a calculation formula for predicting the carbon sink amount based on OD680 is constructed; The OD680 value of the freshwater Chlorella genus collected in real time is input, and the predicted carbon sequestration amount of the freshwater Chlorella genus is output according to the OD680-based predicted carbon sequestration amount calculation formula.

[0007] Preferably, the correlation model is as follows: ; Where x is the OD680 value and P is the biomass.

[0008] Preferably, the calculation formula of the actual carbon sink Fc is as follows: ; Where P is biomass and C% is the carbon content of dry matter of freshwater Chlorella.

[0009] Preferably, the predicted carbon sink based on OD680 The calculation formula is as follows: ; In the formula, is the predicted carbon sink, and x is the OD680 value.

[0010] On the other hand, the present invention also provides a freshwater Chlorella carbon sink calculation system, comprising: A data acquisition module is used to collect the OD680 value and the corresponding biomass of freshwater Chlorella and obtain a correlation model between the biomass and the OD680 value; A carbon sink analysis module is used to obtain the actual carbon sink based on the correlation model and the calculation formula of carbon dioxide absorption; analyze the relationship between the actual carbon sink and the OD680 value, and construct a calculation formula for predicting carbon sink based on OD680; The carbon sink output module is used to input the OD680 value of freshwater Chlorella collected in real time, and output the predicted carbon sink of freshwater Chlorella according to the OD680-based predicted carbon sink calculation formula.

[0011] It can be known from the above technical scheme that compared with the prior art, the present invention discloses a method and system for calculating the carbon sink of freshwater Chlorella, firstly collects the OD680 value and biomass of freshwater Chlorella to obtain a correlation model, then combines the carbon dioxide absorption formula to obtain the actual carbon sink, and then constructs a predicted carbon sink calculation formula, and inputs the real-time OD680 value to output the predicted carbon sink. The present invention estimates the carbon sink in real time by dynamically monitoring the OD680 value, avoids repeated measurement of biomass and carbon content, simplifies the operation process, reduces costs, and can quickly and accurately evaluate the carbon sink potential of freshwater Chlorella, help determine the optimal harvest time, and maximize cost-effectiveness and carbon sink potential. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0013] Figure 1 A flow chart of the method provided by the present invention; Figure 2 This is a system architecture diagram provided by the present invention. DETAILED DESCRIPTION

[0014] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] On the one hand, the embodiment of the present invention provides a method for calculating the carbon sink of freshwater Chlorella, referring to Figure 1 , the present invention comprises the following steps: S1. Collect the OD680 value and corresponding biomass of freshwater Chlorella, and obtain a correlation model between biomass and OD680 value.

[0016] In this embodiment, freshwater Chlorella genus takes Chlorella vulgaris as an example, collects a uniformly mixed algae liquid of freshwater Chlorella genus, and then uses a sterile sampling tool to collect a mixed liquid sample containing freshwater Chlorella vulgaris. Use an ELISA reader to measure the optical density value (OD680) of the freshwater Chlorella vulgaris suspension at a wavelength of 680nm. The optical density value can reflect the content of photosynthetic pigments such as chlorophyll in Chlorella vulgaris cells, and thus indirectly reflect the biomass of Chlorella vulgaris.

[0017] The suspension of Chlorella sphaerocephala with measured OD680 was mixed evenly, 5 ml of the algae solution was extracted and filtered with a pre-dried and weighed 0.22 micron filter membrane, the filter membrane was dried in a 60°C oven for 24 hours, taken out and placed in a dryer to cool to room temperature, and weighed again. The difference between the two is the dry biomass of Chlorella sphaerocephala. The carbon content of the dry algae was determined using an elemental analyzer. A total of 40 samples were collected, with a data range of 47.72% to 50.60%, and the average value was 48.71%.

[0018] The correlation model between the biomass of Chlorella vulgaris and OD680 was made based on a large amount of experimental data as follows: ; Where x is the OD680 value and P is the biomass (g / L).

[0019] S2. Obtain the actual carbon sink based on the correlation model and the calculation formula of carbon dioxide absorption; analyze the relationship between the actual carbon sink and the OD680 value, and construct a calculation formula for predicting carbon sink based on OD680.

[0020] Specifically, the actual carbon sink is obtained by combining the biomass obtained based on the correlation model with the calculation formula of carbon dioxide absorption. , where P is the biomass of Chlorella vulgaris, C% is the carbon content of Chlorella vulgaris stem cells. Through experiments, C% is 48.71%, and 12 and 44 represent the molecular masses of carbon and carbon dioxide, respectively.

[0021] Statistical analysis software was used to analyze the relationship between the actual carbon sink and the OD680 value, and the predicted carbon sink based on OD680 was obtained. The calculation formula is: ; In the formula, is the predicted carbon sink, and x is the OD680 value.

[0022] S3. Input the OD680 value of freshwater Chlorella collected in real time, and output the predicted carbon sink of freshwater Chlorella according to the calculation formula of predicted carbon sink based on OD680.

[0023] The carbon sequestration results can be output once or multiple times according to actual needs and drawn into a graph for analyzing the changing trend of carbon sequestration of freshwater Chlorella in different growth stages.

[0024] During the determination process, three carbon dioxide concentrations were set to culture Chlorella. The OD680 value of the algae solution was measured every three days during the culture process. The carbon sink of Chlorella was calculated by substituting the predicted carbon sink calculation formula based on OD680, and the carbon fixation status of Chlorella was detected. When the carbon sink reached a certain level, Chlorella could be harvested. At the time of harvest, the evenly mixed algae solution was taken to measure the dry biomass of Chlorella, and the actual carbon sink of Chlorella was calculated and compared with the predicted carbon sink, as shown in Table 1.

[0025] Table 1 Predicted and measured values ​​of carbon sink for Chlorella vulgaris

[0026] According to Table 1, 5% CO 2 The predicted values ​​of the concentration group were slightly higher than the measured values, but the difference was small, with an average deviation of +0.225; 10% CO 2 The predicted values ​​of the concentration group were slightly higher than the measured values, but the difference was small, with an average deviation of +0.292; 15% CO 2The predicted values of the concentration groups are close to the measured values, and the prediction of some data points is more accurate, with an average deviation of +0.105.

[0027] From the overall data, the deviation between the predicted values and the measured values is within a reasonable range, and with the increase of CO 2 concentration, the difference between the predicted values and the measured values does not increase significantly. This indicates that the prediction model has a certain stability and reliability.

[0028] On the other hand, as Figure 2 shown, the present invention also provides a carbon sink amount calculation system for freshwater Chlorella, which is used to implement the above-mentioned carbon sink amount calculation method for freshwater Chlorella, including: A data acquisition module, which is used to collect the OD680 value of freshwater Chlorella and the corresponding biomass, and obtain the correlation model between the biomass and the OD680 value; A carbon sink amount analysis module, which is used to obtain the actual carbon sink amount based on the correlation model and the calculation formula of the carbon dioxide absorption amount; analyze the relationship between the actual carbon sink amount and the OD680 value, and construct a prediction carbon sink amount calculation formula based on OD680; A carbon sink amount output module, which is used to input the OD680 value of freshwater Chlorella collected in real time, and output the predicted carbon sink amount of freshwater Chlorella according to the prediction carbon sink amount calculation formula based on OD680.

[0029] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. The same or similar parts among the embodiments can be referred to each other. For the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the description of the method part.

[0030] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for calculating the carbon sink of freshwater Chlorella, characterized in that: The following steps are involved: Collect the OD680 value and corresponding biomass of freshwater Chlorella, and obtain the correlation model between biomass and OD680 value; Obtaining the actual carbon sink amount based on the correlation model and the calculation formula of carbon dioxide absorption amount; Analyze the relationship between the actual carbon sink amount and the OD680 value, and construct a calculation formula for predicting carbon sink amount based on OD680; The OD680 value of the freshwater Chlorella genus collected in real time is input, and the predicted carbon sequestration amount of the freshwater Chlorella genus is output according to the OD680-based predicted carbon sequestration amount calculation formula.

2. The method for calculating the carbon sink of freshwater Chlorella according to claim 1, characterized in that: The correlation model is as follows: ； Where x is the OD680 value and P is the biomass.

3. The method for calculating the carbon sink of freshwater Chlorella according to claim 1, characterized in that: The calculation formula of the actual carbon sink Fc is as follows: ； Where P is biomass and C% is the carbon content of dry matter of freshwater Chlorella.

4. The method for calculating the carbon sink of freshwater Chlorella according to claim 1, characterized in that: The predicted carbon sink based on OD680 The calculation formula is as follows: ； In the formula, To predict carbon sequestration, x is the OD680 value.

5. A freshwater Chlorella carbon sink calculation system, characterized in that: include: A data acquisition module is used to collect the OD680 value and the corresponding biomass of freshwater Chlorella and obtain a correlation model between the biomass and the OD680 value; A carbon sink analysis module, used to obtain the actual carbon sink based on the correlation model and the calculation formula of carbon dioxide absorption; Analyze the relationship between the actual carbon sink amount and the OD680 value, and construct a calculation formula for predicting carbon sink amount based on OD680; The carbon sink output module is used to input the OD680 value of freshwater Chlorella collected in real time, and output the predicted carbon sink of freshwater Chlorella according to the OD680-based predicted carbon sink calculation formula.

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