Preparation method of traditional Chinese medicine for promoting wound healing of pets

Abstract

The traditional Chinese medicine is prepared from the following raw material medicines in parts by weight: 8 to 10 parts of pheretima (pheretima), 8 to 10 parts of calcined dragon bone, 6 to 8 parts of rhizoma bletillae, 6 to 8 parts of frankincense, 6 to 8 parts of myrrh, 4 to 6 parts of rhizoma corydalis, 4 to 6 parts of safflower, 3 to 5 parts of rhizoma chuanxiong, 0.5 to 1 part of preservative phenoxyethanol and an oil-in-water (O / W) emulsion matrix, the oil-in-water (O / W) emulsion matrix comprises 1-2 parts of liquid paraffin, distilled water and 0.1-0.5 part of lauryl sodium sulfate. According to the preparation method of the traditional Chinese medicine for promoting the healing of the pet wound, the traditional Chinese medicine composition for promoting the healing of the pet wound has a remarkable treatment effect, good safety and a convenient use mode, and is an innovative achievement in the field of pet medical treatment.

Description

Technical Field

[0001] The invention relates to the technical field of pet medical treatment, and in particular to a preparation method of a Chinese medicine for promoting pet wound healing. Background Art

[0002] In modern society, with the significant improvement of people's living standards, the role of pets in the family has undergone profound changes. Pets have become an indispensable member of many families. Their health and happiness have received unprecedented attention from their owners. The number of pets kept has also shown a trend of continuous growth, becoming an indispensable emotional sustenance and companion in human life. However, in the process of pet keeping, due to various frequent accidents, such as trauma, surgery, traffic accidents, etc., it is not uncommon for pets to suffer trauma. Due to untimely treatment of trauma, the nature of pets being active and difficult to keep the wound still, and the lack of nursing experience of the owners, the wound healing cycle is often longer, which not only increases the time cost and economic burden of treatment, but also may cause greater pain to the pets due to long-term treatment, causing double pressure on the psychological and economic conditions of the pet owners.

[0003] At present, the products used to promote pet wound healing on the market are mainly divided into two categories: chemical synthetic drugs and traditional Chinese medicine preparations, but both have problems to varying degrees. Although chemical synthetic drugs have certain antibacterial effects, their effects are relatively single, mainly focusing on inhibiting bacterial growth. The effects on key links such as accelerating tissue repair and reducing scar formation are not satisfactory, and it is difficult to fully meet the complex needs of pet wound healing. In addition, chemical drugs may cause a series of side effects, posing a potential threat to the physical health of pets. Some pets may even have allergic reactions to them, further limiting their scope of application in pet wound treatment.

[0004] Although the existing Chinese medicine preparations have exerted the natural advantages of Chinese medicine to a certain extent, there are still many deficiencies in practical application. The efficacy of some Chinese medicine preparations is not stable and significant enough, and it is difficult to achieve the ideal healing effect; in the formulation design, the physiological characteristics and pathological mechanisms of pet wound healing are not fully considered, resulting in unreasonable formulations and failure to give full play to the synergistic effects of Chinese medicine; moreover, some Chinese medicine preparations are not easy to use, which brings additional difficulties and inconveniences to pet owners' care work.

[0005] In view of the above problems, it is particularly urgent to develop a safe, effective and easy-to-use Chinese medicine composition that promotes pet wound healing. This can not only meet the high requirements of pet owners for pet health care, but also has important practical significance and broad market prospects. It will inject new vitality into the development of the pet medical industry and promote pet trauma treatment technology to a new level, so that pets can recover faster after trauma, reduce pain, improve the quality of life of pets, and further enhance the emotional bond between pets and their owners. Summary of the invention

[0006] In view of the above-mentioned deficiencies in the prior art, the present invention provides a method for preparing a Chinese medicine for promoting pet wound healing, the purpose of which is to solve the problems of poor efficacy, unreasonable formulation and inconvenient use.

[0007] In order to achieve the above-mentioned purpose of the invention, the technical scheme adopted by the present invention is: a preparation method of a Chinese medicine for promoting pet wound healing, which is prepared from the following raw materials in parts by weight: 8-10 parts of Guangdilong (Pheretima ternatum), 8-10 parts of calcined dragon bones, 6-8 parts of Bletilla striata, 6-8 parts of frankincense, 6-8 parts of myrrh, 4-6 parts of Corydalis yanhusuo, 4-6 parts of safflower, and 3-5 parts of Chuanxiong.

[0008] Furthermore, the invention also comprises 0.5-1 part of preservative phenoxyethanol and an oil-in-water (O / W) emulsion base.

[0009] Furthermore, the oil-in-water (O / W) emulsion base includes 1-2 parts of liquid paraffin, distilled water, and 0.1-0.5 parts of sodium lauryl sulfate.

[0010] Further, the following steps are included:

[0011] Step 1:

[0012] A1. Cut open the fresh earthworm (Pheretima quinata) and remove the internal organs and impurities;

[0013] A2. Wash away the sand and residue with clean water and drain slightly;

[0014] A3. Spread the processed P. radix and place it in a -20°C refrigerator for pre-freezing until it is completely frozen;

[0015] A4. The pre-frozen Guangdilong was placed in a freeze dryer and freeze-dried at 0-10Pa for 10-12 hours;

[0016] A5. Take out the freeze-dried Dilong and grind it into off-white powder using a medicinal material grinder.

[0017] Step 2:

[0018] Accurately weigh the required medicinal materials, including calcined dragon bone, white radix, corydalis, etc., and mix them according to the preset weight ratio. Place the above-mentioned medicinal materials weighed in proportion in a special mixing container and mix them in a mixing tank until all the medicinal materials are evenly distributed without significant agglomeration. Subsequently, send the evenly mixed medicinal materials into a high-speed grinder for grinding until they are made into ultrafine powder.

[0019] Step 3:

[0020] B1. Mix frankincense, myrrh and rice vinegar in a weight ratio of 10:1;

[0021] B2. Pour the mixed frankincense and myrrh into a wok and fry over low heat until the vinegar is completely absorbed;

[0022] B3. Mix the processed frankincense, myrrh, safflower, chuanxiong and other medicinal materials evenly according to the weight ratio;

[0023] B4. Put the mixed medicinal materials into a grinder and grind them until the particle size is between 20 and 80 meshes;

[0024] B5. The crushed medicinal materials were placed in a supercritical extraction device and extracted for 3 hours at a pressure of 25 MPa, a temperature of 45°C, and a carbon dioxide flow rate of 25 kg / h to obtain a mixed extract.

[0025] Furthermore, the medicinal powder and extracts thereof processed and obtained according to step one, step two and step three are mixed in a mixing tank to ensure that the components are evenly distributed to form a uniform medicinal mixture. The preservative phenoxyethanol is accurately measured and added to the above medicinal mixture in an amount between 0.5 and 1 part of the total mass of the mixture to ensure that the phenoxyethanol is fully dissolved and evenly distributed in the mixture to avoid microbial contamination and extend the shelf life of the ointment. An oil-in-water (O / W) emulsion matrix is ​​prepared, and the components are heated to a suitable temperature to reach the emulsification temperature of each component to promote the full fusion of the components and form a stable emulsion structure.

[0026] Furthermore, after the emulsion matrix reaches a stable state, the medicinal material mixture obtained in step 2 is added to the emulsion matrix at a slow and uniform rate, and at the same time, stirred in a mixing tank, and stirred continuously until the medicinal material mixture and the emulsion matrix are completely fused to form a uniform and delicate paste. This process requires strict control of the stirring speed and the adding speed to avoid generating bubbles or affecting the uniformity of the ointment.

[0027] Furthermore, the prepared Chinese medicine ointment is accurately measured and loaded into a pre-prepared container through a filling device. Subsequently, the container is sealed to prevent external factors such as air and moisture from affecting the quality of the ointment. The sealed ointment should be stored in a cool, dry, light-proof environment to ensure its stability and effectiveness.

[0028] Furthermore, the rotation speed of the mixing tank is 800-1000 rpm.

[0029] The beneficial effects of the present invention are:

[0030] The preparation method of a Chinese medicine for promoting pet wound healing of the present invention has the remarkable characteristics that the Chinese herbal medicine raw materials adopted are not only green and environmentally friendly, free of drug residues, but also ensure no toxic side effects, which fully demonstrates the natural advantages of traditional Chinese medicine. The formula design of the composition is scientific and rigorous, and the compatibility is exquisite. It follows the principle of traditional Chinese medicine of "strengthening the body's resistance and repelling evil, and treating both the symptoms and the root causes", and aims to achieve the purpose of curing diseases by regulating the internal environment of the body.

[0031] In terms of preparation technology, the present invention adopts advanced technologies such as vacuum freeze drying, ultrafine grinding and carbon dioxide supercritical extraction, which can retain the active ingredients in the medicinal materials to the maximum extent, ensuring the full extraction and efficient utilization of the effective ingredients of the medicinal materials. Through the use of these advanced technologies, the present invention successfully constructs the unique "monarch, minister, assistant and messenger" system in the compatibility of traditional Chinese medicine, and achieves the therapeutic goal of strengthening the body and consolidating the foundation.

[0032] In terms of efficacy, the Chinese medicine composition of the present invention shows significant therapeutic effects at different stages of pet trauma. In the early stage of trauma, the composition can quickly converge and stop bleeding, effectively relieve pain, and inhibit the release of inflammatory factors, thereby alleviating inflammatory reactions and creating a gentle and conducive environment for wound healing. In the middle and late stages of trauma, the composition can significantly inhibit the growth and reproduction of pathogens such as Staphylococcus aureus and Escherichia coli, while stimulating the proliferation and migration of fibroblasts, and promoting tissue regeneration and repair. In addition, the composition can also regulate qi and blood, promote blood circulation and remove blood stasis, accurately deliver oxygen and nutrients to wound tissues, and further accelerate the healing process of wounds.

[0033] In terms of dosage form selection, the present invention makes it into an ointment, so that the effective ingredients in each medicinal material can directly act on the wound surface, achieving a slow and lasting release effect. At the same time, the ointment can also form a protective film on the wound surface, effectively preventing the invasion of external bacteria, dust and other pollutants, reducing excessive evaporation of wound moisture, keeping the wound surface moist and clean, and providing a strong guarantee for the rapid healing of the wound.

[0034] It is worth mentioning that compared with traditional Western medicine and frequent injections of antibiotics for anti-inflammatory purposes, the Chinese medicine composition described in the present invention can significantly reduce the stress response to pets, thereby alleviating the tension between owners and pets and improving the quality of life of pets. In summary, the Chinese medicine composition for promoting pet wound healing described in the present invention has significant therapeutic effects, good safety and convenient use, and is an innovative achievement in the field of pet medicine. DETAILED DESCRIPTION

[0035] In order to make the contents of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention.

[0036] A method for preparing a Chinese medicine for promoting wound healing of pets, characterized in that the Chinese medicine comprises the following raw materials, measured by weight: 8-10 parts of Pheretima ternatum (Earthropoda ciliata), 8-10 parts of calcined dragon bones, 6-8 parts of Bletilla striata, 6-8 parts of frankincense, 6-8 parts of myrrh, 4-6 parts of Corydalis yanhusuo, 4-6 parts of safflower, and 3-5 parts of Chuanxiong.

[0037] The invention also comprises 0.5-1 part of preservative phenoxyethanol and an oil-in-water (O / W) emulsion base.

[0038] The oil-in-water (O / W) emulsion base includes 1-2 parts of liquid paraffin, distilled water, and 0.1-0.5 parts of sodium lauryl sulfate.

[0039] Through the above scheme, "Compendium of Materia Medica" records that radix galangae can treat scrofula ulcers and oral erosions, indicating that the ancients have realized that radix galangae has a certain therapeutic effect on some ulcers and traumatic diseases on the body surface. After vacuum freeze-drying, fresh radix galangae can retain effective ingredients such as lumbrokinase and anti-inflammatory active ingredients to the greatest extent, and the structure and performance of antibacterial ingredients can be maintained. Lumbrokinase can activate plasminogen to convert into plasmin, dissolve fibrin at the site of injury, and improve local blood circulation. This is conducive to the transportation of nutrients and oxygen to the wound tissue, providing a good material basis for wound healing, promoting fibroblasts to synthesize collagen, and accelerating the wound healing process. Anti-inflammatory active substances inhibit the release of inflammatory factors such as interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Reduce inflammatory symptoms such as redness, swelling, and pain at the site of injury, reduce the damage to tissues caused by excessive aggregation of inflammatory cells, and create a relatively stable local environment for wound healing. Antibacterial ingredients inhibit the growth and reproduction of common pathogens such as Staphylococcus aureus and Escherichia coli, reducing the probability of wound infection. Vacuum freeze-dried Guangdilong can better play its role in promoting cell proliferation. By regulating cell signaling pathways, it stimulates fibroblasts to synthesize more collagen and provide structural support for new tissues. At the same time, it can also promote the activity of epithelial cells, accelerate wound epithelialization, enable the wound site to complete tissue repair and remodeling faster, and improve the quality and speed of wound healing.

[0040] Calcined dragon bone has the effects of astringent hemostasis, astringent and anti-loosening. In the early stage of trauma, it can shrink the local blood vessels of the wound and reduce blood outflow. It plays a hemostatic role. In the repair stage of trauma, it can absorb wound exudate, keep the wound surface dry, and stimulate the growth of local tissue cells to accelerate the formation of granulation tissue. Bletilla striata is known as the "holy medicine for sores and ulcers". It has the effects of astringent hemostasis, swelling reduction and muscle growth. The main ingredient, Bletilla striata glue, can form a gelatinous protective film on the surface of the wound. This film can block the rupture of blood vessels and prevent blood from flowing out. At the same time, Bletilla striata can also promote platelet aggregation and enhance the hemostatic effect. In the process of tissue repair after trauma, it can inhibit the release of inflammatory mediators and reduce the aggregation of inflammatory cells. It promotes the proliferation and migration of fibroblasts and the synthesis of collagen, thereby promoting the growth of granulation tissue and wound healing. Yuanhu (Yanhusuo) has the effects of promoting blood circulation, dispersing blood stasis, regulating qi and relieving pain, and contains a variety of alkaloids, such as corydalis. It can invigorate blood circulation and remove blood stasis, make the blood flow of the injured part smooth, effectively relieve pain, promote blood circulation, transport nutrients and oxygen to the tissue, facilitate tissue regeneration and repair, and accelerate wound healing. These three medicinal materials are made into ultrafine powder to increase their specific surface area, make the effective ingredients dissolve better, improve their bioavailability, and improve the fineness of the drug. They cooperate with each other in hemostasis, wound protection, and pain relief, and work synergistically to promote the entire process of wound healing.

[0041] Frankincense and myrrh are often used together, and have the effects of promoting blood circulation and relieving pain, reducing swelling and promoting tissue regeneration. After being processed with vinegar, it is more conducive to the release of the effective ingredients of the two. The effect of promoting blood circulation and relieving pain is significantly enhanced, and the effect of reducing swelling and removing blood stasis is better. Chuanxiong is known as the "Qi medicine in the blood", which has the effects of promoting blood circulation and qi, dispelling wind and relieving pain. The Chuanxiong extract extracted by supercritical carbon dioxide mainly contains ingredients such as ligustrazine, which has a strong effect of promoting blood circulation and qi. In the process of wound healing, qi and blood stasis is a common problem. Chuanxiong extract can promote blood circulation and regulate the movement of qi and blood. It can dilate blood vessels and make blood flow smoother, especially for small blood vessels and capillaries in the injured area, which can effectively improve their blood perfusion. By promoting blood circulation and qi, the normal circulation of qi and blood is guaranteed, and delayed wound healing caused by qi and blood stasis is avoided. Safflower has the effects of promoting blood circulation, relieving menstruation, dispersing blood stasis and relieving pain. Safflower contains ingredients such as safflower yellow, which can improve blood circulation, regulate the activity of inflammatory cells, promote the migration and proliferation of epithelial cells, and improve the quality of wound healing. In a supercritical state, the density of carbon dioxide is close to that of liquid, and it has a strong solubility. It can dissolve and extract the active ingredients in frankincense, myrrh, and Chuanxiong. Extraction at a relatively low temperature can effectively avoid chemical reactions such as oxidation and decomposition of heat-sensitive components in medicinal materials due to high temperature, retain the active ingredients and biological activity of medicinal materials to the greatest extent, promote blood circulation and remove blood stasis, and promote tissue regeneration and healing.

[0042] Implementation 1

[0043] 1. Preparation of API

[0044] According to the preparation method of the present invention, the following raw materials need to be prepared first and proportioned according to the specified weight proportions:

[0045] Guangdilong (Pheretima ginseng): 9 portions

[0046] Calcined dragon bone: 9 parts

[0047] Bletilla striata: 7 portions

[0048] Frankincense: 7 parts

[0049] Myrrh: 7 parts

[0050] Yuanhu: 5 portions

[0051] Safflower: 5 parts

[0052] Chuanxiong: 4 servings

[0053] Preservative phenoxyethanol: 0.75 parts

[0054] Oil-in-water (O / W) emulsion base (containing 1.5 parts of liquid paraffin, appropriate amount of distilled water, and 0.3 parts of sodium lauryl sulfate)

[0055] 2. API Processing and Preparation Steps

[0056] Step 1: Treatment of Guangdilong

[0057] A1. Open up the fresh earthworms (Pheretima annuli) and remove the internal organs and impurities to ensure the purity of the raw materials.

[0058] A2. Use clean water to clean the mud and residue on the surface of Guangdilong, and then drain the water slightly to avoid excessive moisture affecting the subsequent freeze-drying process.

[0059] A3. Spread the processed radix radix into a -20℃ refrigerator for pre-freezing until it is completely frozen. The purpose of pre-freezing is to destroy the cell structure of radix radix radix and facilitate subsequent freeze-drying.

[0060] A4. Place the pre-frozen radix radix in a freeze dryer and freeze dry it under vacuum conditions of 0-10Pa for 12 hours. Freeze drying can remove moisture from radix radix radix while retaining its medicinal ingredients and improving the stability of the product.

[0061] A5. Take out the freeze-dried Guangdilong and use a medicinal material grinder to grind it until it becomes an off-white powder. The crushed Guangdilong powder has better solubility and absorption, which helps to improve its efficacy.

[0062] Step 2: Mixing and crushing other medicinal materials

[0063] Accurately weigh other required medicinal materials, including calcined dragon bone, white radix, corydalis, etc., and mix them according to the preset weight ratio. Place the mixed medicinal materials in a special mixing container and use a mixing tank for mixing. The speed of the mixing tank is controlled between 800 and 1000 rpm until all the medicinal materials are evenly distributed without significant agglomeration. Subsequently, the evenly mixed medicinal materials are sent to a high-speed pulverizer for pulverization until ultrafine powder is made. Ultrafine powder has a larger specific surface area and better solubility, which helps to improve the efficacy of the medicine.

[0064] Step 3: Preparation and extraction of frankincense and myrrh

[0065] B1. Mix frankincense, myrrh and rice vinegar in a weight ratio of 10:1. The addition of rice vinegar can soften the medicinal materials and enhance the processing effect.

[0066] B2. Pour the mixed frankincense and myrrh into a wok and fry over low heat until the vinegar is completely absorbed. The frying process can remove impurities and moisture from the herbs and enhance their efficacy.

[0067] B3. The processed frankincense, myrrh, safflower, chuanxiong and other medicinal materials are mixed evenly according to the weight ratio. The addition of these medicinal materials can further enhance the effects of the ointment such as promoting blood circulation and removing blood stasis, reducing swelling and relieving pain.

[0068] B4. Put the mixed medicinal materials into a grinder for grinding until the particle size is between 20 and 80 meshes. The crushed medicinal materials have better solubility and absorption, which helps to improve their efficacy.

[0069] B5. Put the crushed medicinal materials into the supercritical extraction device and perform extraction under the conditions of pressure of 25MPa, temperature of 45℃, carbon dioxide flow rate of 25kg / h, and extraction time of 3 hours. Supercritical extraction technology can efficiently extract the effective ingredients in medicinal materials while avoiding the problem of harmful substance residues that may be caused by traditional extraction methods.

[0070] Step 4: Mixing of medicinal material mixture and emulsion matrix

[0071] The medicinal powder and extracts obtained by processing according to step one, step two and step three are mixed in a mixing tank to ensure that each component is evenly distributed and form a uniform medicinal mixture. Then, the preservative phenoxyethanol is accurately measured and added to the medicinal mixture (the amount added is 0.75 parts of the total mass of the mixture) to ensure that the phenoxyethanol is fully dissolved and evenly distributed in the mixture to extend the shelf life of the ointment and avoid microbial contamination. Then, an oil-in-water (O / W) emulsion matrix is ​​prepared and these components are heated to a suitable temperature (such as 45°C) to reach the emulsification temperature of each component and promote the full fusion of each component to form a stable emulsion structure.

[0072] Step 5: Preparation of ointment

[0073] After the emulsion matrix reaches a stable state, the medicinal material mixture obtained in step 2 is added to the emulsion matrix at a slow and uniform speed. At the same time, a mixing tank is used for stirring (the speed of the mixing tank is controlled between 800 and 1000 rpm), and stirring is continued until the medicinal material mixture and the emulsion matrix are completely fused to form a uniform and delicate paste. In this process, the stirring speed and the adding speed must be strictly controlled to avoid generating bubbles or affecting the uniformity of the ointment.

[0074] Step 6: Filling and sealing

[0075] The prepared Chinese medicine ointment is accurately measured by the filling equipment and filled into the pre-prepared container. The container is then sealed to prevent external factors such as air and moisture from affecting the quality of the ointment. The sealed ointment should be stored in a cool, dry, light-proof environment to ensure its stability and effectiveness.

[0076] 3. Implementation Effect

[0077] The Chinese medicine ointment prepared according to this embodiment has significant curative effect and wide application prospects. The ointment can quickly promote pet wound healing, relieve pet pain and accelerate the recovery process. At the same time, the ointment also has good stability and safety and is suitable for the treatment of various pet wounds.

[0078] Comparative Example

[0079] In order to verify the importance of the raw material ratio and preparation steps in the present invention, this comparative example adopts a method of changing the raw material for comparison. The following is the specific implementation process of this comparative example:

[0080] 1. Preparation of API

[0081] The preparation process of the raw material medicine is different from that in the embodiment, as follows:

[0082] Guangdilong (Earthropoda cernua): Replace with an equal amount of dried earthworms (naturally sun-dried or oven-dried)

[0083] Forged dragon bone: replaced with an equal amount of raw dragon bone

[0084] White jellyfish: remain unchanged

[0085] Frankincense: Remains the same

[0086] Myrrh: Stay the same

[0087] Yuanhu: Replace with an equal amount of Panax notoginseng powder

[0088] Red flower: remain unchanged

[0089] Chuanqiong: Remain unchanged

[0090] Preservative Phenoxyethanol: Unchanged

[0091] Oil-in-water (O / W) emulsion base: remains unchanged

[0092] 2. API Processing and Preparation Steps

[0093] Step 1: Processing of Dried Earthworms

[0094] Since the dried earthworm is already in a dry state, there is no need to freeze-dry it. It can be directly crushed until it becomes powdery.

[0095] Step 2: Mixing and crushing other medicinal materials

[0096] The replaced medicinal materials (raw dragon bone, Panax notoginseng powder, etc.) are mixed with other unreplaced medicinal materials (Bletilla striata, frankincense, myrrh, safflower, Chuanxiong) according to a preset weight ratio. The mixing and crushing process is the same as in the embodiment.

[0097] Step 3: Preparation and extraction of frankincense and myrrh

[0098] The preparation and extraction process of frankincense and myrrh are the same as those in the embodiment.

[0099] Step 4: Mixing of medicinal material mixture and emulsion matrix

[0100] The process of mixing the processed medicinal material powder and its extract with the emulsion matrix is ​​the same as in the embodiment.

[0101] Step 5: Preparation of ointment

[0102] The ointment preparation process is the same as that in the embodiment.

[0103] Step 6: Filling and sealing

[0104] The filling and sealing process is the same as in the embodiment.

[0105] 3. Comparison of Implementation Effects

[0106] By comparing the efficacy of the Chinese medicine ointments prepared in the embodiment and the comparative example, it was found that the ointment prepared using the raw material drug ratio and preparation steps in the embodiment has a faster wound healing speed and better therapeutic effect; while the ointment in the comparative example has a relatively poor efficacy due to the replacement of some raw materials.

[0107] Compared with the comparative example, the experimental example significantly shortened the healing course, and the scar left on the wound surface was not obvious, which shows that the raw material drug ratio and preparation steps in the present invention have an important influence on improving the efficacy of the ointment. At the same time, by testing the stability and safety of the ointment, it was found that the ointment prepared in the embodiment has better stability and safety and is suitable for the treatment of various pet injuries.

[0108] In summary, the Chinese medicine preparation method for promoting pet wound healing provided by the present invention has significant therapeutic effect and broad application prospects. By adopting specific raw material drug ratios and preparation steps and supercritical extraction technology and other means, a Chinese medicine preparation that can quickly promote pet wound healing, relieve pet pain and accelerate the recovery process can be prepared. The preparation method is simple, easy to implement, and highly operable, and the prepared ointment has excellent therapeutic effect and stability, which is suitable for industrial production and promotion and application in the field of pet medicine.

[0109] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a Chinese medicine for promoting wound healing in pets, characterized in that: The invention is prepared from the following raw materials in parts by weight: 8-10 parts of Pheretima ternatum (Earthropoda quinata), 8-10 parts of calcined dragon bones, 6-8 parts of Bletilla striata, 6-8 parts of frankincense, 6-8 parts of myrrh, 4-6 parts of Corydalis yanhusuo, 4-6 parts of safflower, and 3-5 parts of Chuanxiong.

2. A method for preparing a Chinese medicine for promoting wound healing in pets according to claim 1, characterized in that: The invention also comprises 0.5-1 part of preservative phenoxyethanol and an oil-in-water (O / W) emulsion base.

3. A method for preparing a Chinese medicine for promoting wound healing in pets according to claim 1, characterized in that: The oil-in-water (O / W) emulsion base comprises 1-2 parts of liquid paraffin, distilled water and 0.1-0.5 parts of sodium lauryl sulfate.

4. A method for preparing a Chinese medicine for promoting wound healing in pets according to any one of claims 1 to 3, characterized in that: The following steps are included: Step 1: A1. Cut open the fresh earthworm (Pheretima quinata) and remove the internal organs and impurities; A2. Wash away the sand and residue with clean water and drain slightly; A3. Spread the processed P. radix and place it in a -20°C refrigerator for pre-freezing until it is completely frozen; A4. The pre-frozen Guangdilong was placed in a freeze dryer and freeze-dried at 0-10Pa for 10-12 hours; A5. Take out the freeze-dried Dilong and grind it into off-white powder using a medicinal material grinder. Step 2: Accurately weigh the required medicinal materials, including calcined dragon bone, white radix, corydalis, etc., and mix them according to the preset weight ratio. Place the above-mentioned medicinal materials weighed in proportion in a special mixing container and mix them in a mixing tank until all the medicinal materials are evenly distributed without significant agglomeration. Subsequently, send the evenly mixed medicinal materials into a high-speed grinder for grinding until they are made into ultrafine powder. Step 3: B1. Mix frankincense, myrrh and rice vinegar in a weight ratio of 10:1; B2. Pour the mixed frankincense and myrrh into a wok and fry over low heat until the vinegar is completely absorbed; B3. Mix the processed frankincense, myrrh, safflower, chuanxiong and other medicinal materials evenly according to the weight ratio; B4. Put the mixed medicinal materials into a grinder and grind them until the particle size is between 20 and 80 meshes; B5. The crushed medicinal materials were placed in a supercritical extraction device and extracted for 3 hours at a pressure of 25 MPa, a temperature of 45°C, and a carbon dioxide flow rate of 25 kg / h to obtain a mixed extract.

5. A method for preparing a Chinese medicine for promoting wound healing in pets according to claim 4, characterized in that: The medicinal material powder and extracts processed and obtained according to step one, step two and step three are mixed in a mixing tank to ensure that the components are evenly distributed to form a uniform medicinal material mixture. The preservative phenoxyethanol is accurately measured and added to the above medicinal material mixture in an amount between 0.5 parts and 1 part of the total mass of the mixture to ensure that the phenoxyethanol is fully dissolved and evenly distributed in the mixture to avoid microbial contamination and extend the shelf life of the ointment. An oil-in-water (O / W) emulsion matrix is ​​prepared, and the components are heated to an appropriate temperature to reach the emulsification temperature of each component to promote the full fusion of the components and form a stable emulsion structure.

6. A method for preparing a Chinese medicine for promoting wound healing in pets according to claim 5, characterized in that: After the emulsion matrix reaches a stable state, the medicinal material mixture obtained in step 2 is added to the emulsion matrix at a slow and uniform rate. At the same time, stirring is performed using a mixing tank. Stirring is continued until the medicinal material mixture and the emulsion matrix are completely fused to form a uniform and delicate paste. During this process, the stirring speed and the adding speed must be strictly controlled to avoid generating bubbles or affecting the uniformity of the ointment.

7. A method for preparing a Chinese medicine for promoting wound healing in pets according to claim 6, characterized in that: The prepared Chinese medicine ointment is accurately measured and loaded into a pre-prepared container through a filling equipment. Subsequently, the container is sealed to prevent external factors such as air and moisture from affecting the quality of the ointment. The sealed ointment should be stored in a cool, dry, light-proof environment to ensure its stability and effectiveness.

8. The method for preparing a Chinese medicine for promoting wound healing of pets according to claim 6, characterized in that: The rotating speed of the mixing tank is 800-1000 rpm.