Microfluidic device and microfluidic detection device

Abstract

A microfluidic device (100) and a microfluidic detection device (1000), the microfluidic device (100) comprising a body (110) and a flow channel switching assembly (120); the body (110) is provided with a second flow channel (P2) and a sealed chamber (R), the second flow channel (P2) comprises a second flow channel first branch (P21), a second flow channel second branch (P22) and a second flow channel main trunk (P23), the second flow channel first branch (P21) and the second flow channel second branch (P22) meet at the second flow channel main trunk (P23), the sealed chamber (R) is located below the second flow channel first branch (P21), and the fluid in the sealed chamber (R) and the fluid in the second flow channel first branch (P21) can perform ion transfer; the flow channel switching assembly (120) is rotationally connected to the body (110) for making the fluid in the second flow channel main trunk (P23) flow to the second flow channel first branch (P21) and / or the second flow channel second branch (P22). The microfluidic detection device (1000) assembled based on the microfluidic device (100) can select different branches (P21, P22) according to requirements to adapt to different biochemical reaction stages required, and the application scenarios are wider.

Description

Technical Field

[0001] This invention belongs to the field of microfluidic control technology, and in particular to a microfluidic device and a microfluidic detection device. Background Technology

[0002] Microfluidics is a technology for controlling, manipulating, and detecting complex fluids at the microscopic level. It is a novel interdisciplinary field developed based on microelectronics, micromechanics, bioengineering, and nanotechnology. Fluid manipulation is frequently required in scientific experiments in biology, chemistry, and materials science. Operations such as DNA sample preparation, liquid chromatography, PCR reactions, and electrophoresis are all performed in a liquid environment. If sample preparation, biochemical reactions, and result detection are integrated onto a biochip, the volume of fluid used in the experiment can be reduced from milliliters to microliters. This makes powerful microfluidic devices indispensable. Due to their advantages such as compact size, small sample / reagent volume usage, fast reaction speed, large-scale parallel processing, and disposable design, their applications in biotechnology research are very extensive.

[0003] Microfluidic detection devices require pre-packaged detection solutions for use in detection. During the detection process using microfluidic detection devices, different reagents need to be injected into the microfluidic detection devices to meet the needs of different stages of the biochemical reaction.

[0004] For some biochemical reactions, the reagents introduced into the microfluidic detection device at different stages do not all need to form a sensing signal with the pre-packaged detection liquid. However, the existing microfluidic detection devices have a simple channel layout, which makes it difficult to be compatible with the requirements of different biochemical reaction stages and limit the application scenarios. Summary of the Invention

[0005] This invention provides a microfluidic device and a microfluidic detection device to solve the problems of incompatibility with different biochemical reaction stages and limited application scenarios in the prior art.

[0006] To solve the above problems, the present invention adopts the following solution:

[0007] In a first aspect, the present invention provides a microfluidic device comprising a body and a flow channel switching assembly; the body is provided with a second flow channel and a sealed chamber, the second flow channel including a first branch of the second flow channel, a second branch of the second flow channel and a main flow channel, the first branch of the second flow channel and the second branch of the second flow channel converging at the main flow channel, the sealed chamber being located below the first branch of the second flow channel, and the fluid in the sealed chamber being capable of ion transfer with the fluid in the first branch of the second flow channel; the flow channel switching assembly is rotatably connected to the body for causing the fluid in the main flow channel to flow to the first branch of the second flow channel and / or the second branch of the second flow channel.

[0008] In an optional embodiment of the present invention, the main body is provided with multiple flow channels, including a third flow channel and a second flow channel; the flow channel switching component is provided with a first liquid passage and a second liquid passage; the flow channel switching component is configured such that when rotated to the second position, the first liquid passage connects the second branch of the second flow channel with the third flow channel; the flow channel switching component is configured such that when rotated to the fourth position, the second liquid passage connects the first branch of the second flow channel with the third flow channel.

[0009] In an optional embodiment of the present invention, the third flow channel is a flow channel that extends in a roundabout manner and is used to collect fluid.

[0010] In an optional embodiment of the present invention, the third flow channel includes an eighth flow channel port, which is located on one side of the flow channel switching assembly and is used to discharge fluid from the third flow channel.

[0011] In an optional embodiment of the present invention, the multiple flow channels further include a fourth flow channel, and the flow channel switching component is configured such that when rotated to the third position, the second liquid passage connects the third flow channel and the fourth flow channel.

[0012] In an optional embodiment of the present invention, the fourth flow channel includes a tenth flow channel port, which is located on one side of the flow channel switching component.

[0013] In an optional embodiment of the present invention, the plurality of flow channels further includes a first flow channel; the flow channel switching component is further provided with a third liquid passage, a fourth liquid passage, and a fifth liquid passage; the flow channel switching component is configured such that when rotated to the first position, the fourth liquid passage connects the first flow channel and the main path of the second flow channel; when rotated to the second position, the third liquid passage connects the first flow channel and the main path of the second flow channel; and when rotated to the fourth position, the fifth liquid passage connects the first flow channel and the main path of the second flow channel.

[0014] In an optional embodiment of the present invention, the first flow channel includes a first flow channel opening, which is located on one side of the flow channel switching component; the microfluidic device further includes a fluid storage component, which is connected to the body and communicates with the first flow channel opening.

[0015] In an optional embodiment of the present invention, the microfluidic device further includes a membrane disposed between the sealed chamber and the first branch of the second flow channel, and the membrane is configured to allow ions to pass through.

[0016] In an optional embodiment of the present invention, the first branch of the second flow channel is provided with a flow channel buffer, which is gradually expanded and then gradually narrowed along the extension direction of the first branch of the second flow channel; the sealing chamber is located below the flow channel buffer, and the membrane is located between the sealing chamber and the flow channel buffer.

[0017] In an optional embodiment of the present invention, the main body is provided with multiple flow channels, including a second flow channel; the main body includes an upper plate, a flow channel plate and a lower plate, and the upper and lower surfaces of the flow channel plate are provided with flow channel grooves; the upper plate and the lower plate are respectively disposed on the upper and lower sides of the flow channel plate to form multiple flow channels together with the flow channel grooves.

[0018] In an optional embodiment of the present invention, the plurality of flow channels further includes a first flow channel; the flow channel groove includes a first flow channel groove, the first flow channel groove includes a first section of the first flow channel groove and a second section of the first flow channel groove; the first section of the first flow channel groove and the second section of the first flow channel groove are connected and are respectively located on the upper surface and the lower surface of the flow channel plate, and the first flow channel groove together with the upper plate and the lower plate form a first flow channel.

[0019] In an optional embodiment of the present invention, the multiple flow channels further include a third flow channel; the flow channel groove includes a third flow channel groove, which includes a first section, a second section, and a third section; the first section and the third section are located on the upper surface of the flow channel plate, and the second section is located on the lower surface of the flow channel plate; the first section, the second section, and the third section are sequentially connected and together with the upper plate and the lower plate form a third flow channel.

[0020] In an optional embodiment of the present invention, the plurality of flow channels further includes a fourth flow channel; the flow channel groove includes a fourth flow channel groove, which is located on the upper surface of the flow channel plate and forms a fourth flow channel together with the upper plate.

[0021] In an optional embodiment of the present invention, the second flow channel main path includes a second flow channel first section and a second flow channel second section; the flow channel groove includes a second flow channel groove, which includes a second flow channel groove first section, a second flow channel groove second section, and a second flow channel groove third section; the second flow channel groove first section and the second flow channel groove second section are located on the lower surface of the flow channel plate, and the second flow channel groove third section is located on the upper surface of the flow channel plate; the second flow channel first section and the lower plate together form the second flow channel first section, the second flow channel groove second section and the second flow channel groove third section are connected and together with the upper plate and the lower plate form the second flow channel second section; the second flow channel first section includes a first liquid outlet, the second flow channel second section includes a second liquid outlet, the first liquid outlet and the second liquid outlet are arranged alternately on the lower plate, and the second flow channel first section and the second flow channel second section can be connected through the first liquid outlet and the second liquid outlet.

[0022] In an optional embodiment of the present invention, the second flow channel further includes a fourth section and a fifth section of the second flow channel, the fourth section and the fifth section of the second flow channel are located on the upper surface of the flow channel plate; the fourth section of the second flow channel is connected to the third section of the second flow channel and forms a first branch of the second flow channel with the upper plate; the fifth section of the second flow channel is connected to the third section of the second flow channel and forms a second branch of the second flow channel with the upper plate.

[0023] In an optional embodiment of the present invention, the first section of the flow channel is provided with a first buffer zone; the second section of the flow channel is provided with a second buffer zone; the shape of the first buffer zone gradually expands from the side away from the first liquid outlet to the side closer to the first liquid outlet; the shape of the second buffer zone gradually expands from the side away from the second liquid outlet to the side closer to the second liquid outlet.

[0024] In an optional embodiment of the present invention, a liquid storage tank is provided on the lower surface of the flow channel plate; the liquid storage tank is sealed by a membrane and a lower plate to form a sealed chamber; the membrane is disposed between the liquid storage tank and the first branch of the second flow channel; and a conductive sheet is provided on the lower plate at the position where it overlaps with the liquid storage tank in the vertical direction.

[0025] In an optional embodiment of the present invention, the flow channel switching assembly includes an upper sealing plate and a lower sealing plate, which are respectively attached to the upper and lower sides of the body. The first liquid passage groove and the second liquid passage groove are disposed on the upper sealing plate, and the third liquid passage groove, the fourth liquid passage groove and the fifth liquid passage groove are disposed on the lower sealing plate.

[0026] In an optional embodiment of the present invention, the first flow channel includes a second flow channel opening disposed on the lower surface of the body; the main flow channel of the second flow channel includes a third flow channel opening disposed on the lower surface of the body; wherein the second flow channel opening and the third flow channel opening are overlapped with the lower sealing plate in the vertical direction.

[0027] In an optional embodiment of the present invention, the first branch of the second flow channel includes a fourth flow channel opening disposed on the upper surface of the body; the second branch of the second flow channel includes a fifth flow channel opening disposed on the upper surface of the body; the third flow channel includes a sixth and a seventh flow channel opening disposed on the upper surface of the body; the fourth flow channel includes a ninth flow channel opening disposed on the upper surface of the body; the fourth, fifth, sixth, seventh, and ninth flow channel openings are overlapped with the upper sealing plate in the vertical direction.

[0028] In an optional embodiment of the present invention, the main body is provided with a limiting structure; the flow channel switching assembly further includes a limiting component. During the rotation of the flow channel switching assembly relative to the main body, the stopping position of the limiting component is determined by the limiting structure, so that the flow channel switching assembly switches between the first position, the second position, the third position and the fourth position.

[0029] In an optional embodiment of the present invention, the limiting mechanism includes a first limiting groove, a second limiting groove, and a slide groove; the limiting component is configured such that when switched to the first limiting groove, the flow channel switching component is located in the first position; the limiting component is configured such that when switched to the second limiting groove, the flow channel switching component is located in the second position; the limiting component is configured to slide along the slide groove to switch the flow channel switching component between the third position and the fourth position.

[0030] In an optional embodiment of the present invention, the chute includes a first chute section and a second chute section; the limiting component is configured to slide along the first chute section to rotate the flow channel switching component to a third position; the limiting component is configured to slide along the second chute section to rotate the flow channel switching component to a fourth position.

[0031] In an optional embodiment of the present invention, the groove depth is greater than the groove depth of the first limiting groove and the second limiting groove.

[0032] In an optional embodiment of the present invention, the flow channel switching assembly further includes a rotating body and a rotating shaft. The rotating body is rotatably connected to the main body via the rotating shaft. The limiting component and the upper sealing plate are connected to the rotating body and can rotate with the rotating body. The two ends of the rotating shaft are respectively connected to the rotating body and the lower sealing plate, and the lower sealing plate can rotate with the rotating shaft.

[0033] In a second aspect, the present invention provides a microfluidic detection device, which includes a sensing device and the aforementioned microfluidic device; the sensing device is disposed at the main channel of the second flow channel and connected to the body, and the sensing device and the body together form a sensing area in the corresponding part of the main channel of the second flow channel.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The microfluidic device provided by the present invention includes a body and a flow channel switching component. The body is provided with a second flow channel. By setting the second flow channel as a bifurcated flow channel structure and cooperating with the flow channel switching component, the fluid in the second flow channel can flow to different branches. The microfluidic detection device assembled based on this microfluidic device can select different branches according to the needs to adapt to the requirements of different biochemical reaction stages, and has a wider range of application scenarios. Attached Figure Description

[0036] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0037] Figure 1a This is a schematic diagram of a microfluidic detection device provided according to one embodiment of the present disclosure;

[0038] Figure 1b for Figure 1a A cross-sectional view of the microfluidic detection device in the image;

[0039] Figure 1c for Figure 1a One of the exploded diagrams of a microfluidic detection device in China;

[0040] Figure 1d for Figure 1a Exploded view of the microfluidic detection device in the image (Part 2);

[0041] Figure 2a for Figure 1a A schematic diagram of a microfluidic device;

[0042] Figure 2b for Figure 2a One of the exploded diagrams of a microfluidic device in China;

[0043] Figure 2c for Figure 2a Exploded view of a microfluidic device (Part 2);

[0044] Figure 2d for Figure 2c Exploded view of a microfluidic device in China (Part 3);

[0045] Figure 2e for Figure 2c Enlarged view of part Q in the image;

[0046] Figure 2f for Figure 2d Enlarged view of section X in the image;

[0047] Figure 3a for Figure 2a A schematic diagram of the flow channel switching component;

[0048] Figure 3b for Figure 3a Exploded view of the flow channel switching component in the middle;

[0049] Figure 4a This is a partial exploded view of a microfluidic device provided according to one embodiment of the present disclosure;

[0050] Figure 4b for Figure 4a Another exploded view of the microfluidic device in the image;

[0051] Figure 4c for Figure 4b An exploded view of a microfluidic device from another perspective;

[0052] Figure 5a A simplified diagram of the flow channel layout inside the body is provided according to one embodiment of the present invention;

[0053] Figure 5b A simplified diagram showing the connectivity of different flow channels when the flow channel switching component is rotated to the first position;

[0054] Figure 5c A simplified diagram showing the connectivity of different flow channels when the flow channel switching component is rotated to the second position;

[0055] Figure 5d A simplified diagram showing the connectivity of different flow channels when the flow channel switching component is rotated to the third position;

[0056] Figure 5e A simplified diagram showing the connectivity of different flow channels when the flow channel switching component is rotated to the fourth position;

[0057] Figure 6 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid passage groove, respectively, when the limiting component is located in the first limiting groove.

[0058] Figure 7 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid passage groove, respectively, when the limiting component is located in the second limiting groove.

[0059] Figure 8 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid passage groove, respectively, when the limiting component is located at one end of the slide groove near the second limiting groove.

[0060] Figure 9 Figures (a) and (b) respectively show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate and the lower liquid passage groove when the limiting component is located at one end of the slide groove near the first limiting groove.

[0061] The components represented by each number in the above attached diagram are listed below:

[0062] 1000. Microfluidic detection device;

[0063] 100. Microfluidic device; 110. Body; 111. Upper plate; 112. Flow channel plate; 113. Lower plate; 1131. First guide post; 1132. Second guide post; 120. Flow channel switching assembly; 121. Rotating body; 122. Rotating shaft; 1221. Tray; 123. Sealing plate; 124. Limiting component; 130. Fluid storage component; 140. Membrane; 150. Conductive sheet; 160. Sealing sticker;

[0064] 200. Sensing device; 210. Carrier board; 220. Sensing chip; 230. Probe;

[0065] 300. Sealing gasket;

[0066] P1, First flow channel; P2, Second flow channel; P21, First branch of the second flow channel; P22, Second branch of the second flow channel; P23, Main road of the second flow channel; P231, First section of the second flow channel; P232, Second section of the second flow channel; P233, Third section of the second flow channel; P3, Third flow channel; P4, Fourth flow channel;

[0067] C1, First flow channel opening; C2, Second flow channel opening; C3, Third flow channel opening; C4, Fourth flow channel opening; C5, Fifth flow channel opening; C6, Sixth flow channel opening; C7, Seventh flow channel opening; C8, Eighth flow channel opening; C9, Ninth flow channel opening; C10, Tenth flow channel opening; C11, Eleventh flow channel opening; C12, Twelfth flow channel opening;

[0068] B1, First liquid transfer tank; B2, Second liquid transfer tank; B3, Third liquid transfer tank; B4, Fourth liquid transfer tank; B5, Fifth liquid transfer tank;

[0069] V1, First limiting groove; V2, Second limiting groove; V3, Sliding groove;

[0070] G1, First flow channel; G11, First section of first flow channel; G12, Second section of first flow channel; G2, Second flow channel; G21, First section of second flow channel; G211, First buffer zone; G22, Second section of second flow channel; G221, Second buffer zone; G23, Third section of second flow channel; G24, Fourth section of second flow channel; G25, Fifth section of second flow channel; G3, Third flow channel; G31, First section of third flow channel; G32, Second section of third flow channel; G33, Third section of third flow channel; G4, Fourth flow channel;

[0071] H1, First through hole; H2, Second through hole; H3, Third through hole; H4, Fourth through hole; H5, Fifth through hole; H6, Sixth through hole;

[0072] D1, first liquid outlet; D2, second liquid outlet;

[0073] L, liquid storage tank; R, sealed chamber; K, shaft insertion hole;

[0074] A1, Sensing area; A2, Flow channel buffer zone; A3, Observation area. Detailed Implementation

[0075] To make the above and other features and advantages of the present invention clearer, the invention will be further described below with reference to the accompanying drawings. It should be understood that the specific embodiments given herein are for the purpose of explanation to those skilled in the art and are exemplary only, not restrictive.

[0076] Figure 1aThis is a schematic diagram of a microfluidic detection device 1000 provided according to one embodiment of the present disclosure. Please refer to... Figure 1a The microfluidic detection device 1000 includes a microfluidic device 100 and a sensing device 200, wherein the sensing device 200 is connected to the microfluidic device 100 and is located below the microfluidic device 100.

[0077] Figure 2a for Figure 1a A schematic diagram of the microfluidic device 100 is shown below. Please refer to [link / reference]. Figure 2a The microfluidic device 100 includes a body 110 and a flow channel switching assembly 120. The body 110 has multiple flow channels. Due to the complex flow channel layout, for ease of understanding of the present disclosure, Figure 5a An example is provided: a simplified diagram of the flow channel layout inside the body 110.

[0078] Please see Figure 5a The main body 110 is provided with a first flow channel P1, a second flow channel P2, a third flow channel P3, a fourth flow channel P4 and a sealed chamber R. Each flow channel is independent of each other and includes multiple flow channel openings.

[0079] In this disclosure, the first flow channel P1 includes a first flow channel opening C1 and a second flow channel opening C2, the second flow channel P2 includes a third flow channel opening C3, a fourth flow channel opening C4 and a fifth flow channel opening C5, the third flow channel P3 includes a sixth flow channel opening C6, a seventh flow channel opening C7 and an eighth flow channel opening C8, and the fourth flow channel includes a ninth flow channel opening C9 and a tenth flow channel opening C10.

[0080] The second flow channel P2 includes a first branch P21, a second branch P22, and a main flow channel P23. The first branch P21 and the second branch P22 converge at the main flow channel P23. Therefore, in this disclosure, the second flow channel P2 is a bifurcated flow channel forming two branches. The main flow channel P23 includes a first section P231 and a second section P232. The first section P231 includes a first liquid outlet D1, and the second section P232 includes a second liquid outlet D2. The first liquid outlet D1 and the second liquid outlet D2 are arranged at intervals and located at the ends of the first section P231 and the second section P232, respectively.

[0081] Figure 1b for Figure 1a A cross-sectional view of the microfluidic detection device 1000 in the middle. Figure 1c for Figure 1a One of the exploded views of the microfluidic detection device 1000 in China. Figure 1d for Figure 1a Exploded view of the microfluidic detection device 1000 (Part 2). Please refer to [link / reference]. Figures 1b to 1dIn this disclosure, the sensing device 200 is connected to the body 110. The first liquid outlet D1 and the second liquid outlet D2 are disposed on the lower surface of the body 110. The vertical projection of the sensing device 200 covers the first liquid outlet D1 and the second liquid outlet D2, forming a third section P233 of the second flow channel. The third section P233 connects the first section P231 and the second section P232 of the second flow channel to form a complete main channel P23 of the second flow channel. Understandably, fluid passing through the third section P233 of the second flow channel can be detected by the sensing device 200 and generate a signal. Therefore, a sensing area A1 is formed in the third section P233 of the second flow channel.

[0082] Please see Figure 1a The main body 110 is provided with a transparent observation area A3, which is located above the sensing area A1. In specific applications, the projection of the sensing area A3 in the vertical direction covers the first flow channel opening D1 and the second flow channel opening D2, so as to facilitate the observation of the fluid conditions at the sensing area A1.

[0083] Please see Figures 1b to 1d In order to prevent fluid leakage in the third section P233 of the second flow channel, the microfluidic detection device 1000 in this disclosure also includes a sealing gasket 300. The sealing gasket 300 is located between the body 110 and the sensing device 200. The sealing gasket 300 has an annular structure and is located outside the liquid inlet, surrounding the sensing area A1.

[0084] In this disclosure, the main body 110 extends downward with a sealing gasket guide post. The sealing gasket guide post includes a first sealing gasket guide post 1131 and a second sealing gasket guide post 1132 arranged in parallel at intervals. The sealing gasket 300 is provided with a guide post insertion hole. The installation position of the sealing gasket 300 is determined by the cooperation between the sealing gasket guide post and the guide post insertion hole, so as to achieve quick installation.

[0085] It should be noted that the sealed chamber R can pre-seal the fluid. In this disclosure, the sealed chamber R is located below the first branch of the second flow channel P21. The fluid sealed in the sealed chamber R and the fluid flowing through the first branch of the second flow channel P21 can undergo ion transfer, thereby forming a detectable signal.

[0086] In practical applications, a membrane 140 is provided between the sealed chamber R and the first branch of the second flow channel P21. The membrane 140 separates the sealed chamber R from the first branch of the second flow channel P21. The membrane 140 is configured to allow ions to pass through, thus ensuring that only ion transfer occurs between the fluid in the sealed chamber R and the fluid in the first branch of the second flow channel P21. The membrane 140 can be a semi-permeable membrane or a highly permeable membrane, such as an ion exchange membrane or a proton exchange membrane, as long as it allows ions to pass through; there are no specific restrictions.

[0087] Please see Figure 5aFurthermore, the first branch of the second flow channel P21 is provided with a flow channel buffer zone A2. The flow channel buffer zone A2 is set to gradually expand and then gradually narrow along the extension direction of the first branch of the second flow channel P21. It can be understood that the groove width at the flow channel buffer zone A2 is wider, and correspondingly, the fluid velocity at the flow channel buffer zone A2 is reduced. Preferably, the sealing chamber R is located below the flow channel buffer zone A2, and the membrane 140 is located between the sealing chamber R and the flow channel buffer zone A2. In this way, the fluid in the first branch of the second flow channel P21 is decelerated in the flow channel buffer zone A2, avoiding excessively high flow velocity from affecting ion exchange with the fluid in the sealing chamber R, and ensuring signal stability. It can be seen that the cross-sectional shape of the flow channel can be continuous or varied, and there is no specific limitation.

[0088] Please see Figure 1d In this disclosure, the body 110 also includes a conductive sheet 150 located below the membrane 140, through which signals generated by ion exchange in the sealed chamber R can be transmitted to the outside.

[0089] Please see Figure 1c In this disclosure, the sensing device 200 includes a carrier plate 210, a sensing chip 220, and a probe 230. As previously described, the sealing gasket 300 has an annular structure and is disposed around the sensing area A1, thus forming a sealing gasket opening. The sensing area A1 is located within the sealing gasket opening, and the upper surface of the sensing chip 220 can be embedded within the sealing gasket opening. It is evident that the sensing chip 220 is located within the sensing area A1 and can be wetted by the fluid in the third section P233 of the second flow channel.

[0090] The probe 230 is connected to the carrier plate 210 and its top end is connected to the conductive sheet 150. The signal transmitted by the conductive sheet 150 is transmitted to the carrier plate 210 through the probe 230. In addition, the signal generated by the sensing chip 220 is also transmitted to the carrier plate 210. The carrier plate 210 transmits the signal to the signal acquisition and analysis instrument to complete the detection and analysis.

[0091] It should be noted that the conductive sheet 150 can be of various forms, such as a copper alloy sheet or an aluminum alloy sheet, and the probe 230 can also be of various forms, as long as it can form an electrical connection with the conductive sheet 150. The carrier board 210 can be a PCB board, ensuring signal transmission with the sensing chip 220 and the probe 230. The sensing chip 220 can be a biochip, and the sensing chip 220 is integrated on the carrier board 210 to form the main body of the sensing device 200.

[0092] Please see Figure 5aIn this disclosure, each flow channel opening is located at the end of its corresponding flow channel. Specifically, the third flow channel opening C3 is located at the end of the main flow path P23 of the second flow channel, the fourth flow channel opening C4 is located at the end of the first branch flow path P21 of the second flow channel, and the fifth flow channel opening C5 is located at the end of the second branch flow path P22 of the second flow channel. It should be noted that the flow channel openings can also be located in the middle of the corresponding flow channel, and the specific location of the flow channel openings in the flow channel is not limited.

[0093] In this disclosure, the flow channel switching assembly 120 is rotatably connected to the body 110, for example, through a shaft or gear. The flow channel switching assembly 120 is used to switch the connection of different flow channels. Specifically, the flow channel switching assembly 120 is provided with a liquid passage groove. The flow channel switching assembly 120 is configured to rotate relative to the body 110 to adjust the position of the liquid passage groove so that at least two of the multiple flow channels are connected via the liquid passage groove. It can be understood that during the rotation of the flow channel switching assembly 120 relative to the body 110, the liquid passage groove rotates with the flow channel switching assembly 120 to adjust its position. Figures 5b to 5e This is a simplified diagram showing the connectivity of different flow channels when the flow channel switching component 120 rotates to different special positions. Please refer to... Figures 5b to 5e The flow channel switching assembly 120 is provided with a first liquid passage tank B1, a second liquid passage tank B2, a third liquid passage tank B3, a fourth liquid passage tank B4 and a fifth liquid passage tank B5.

[0094] Please see Figure 5b When the flow channel switching component 120 rotates to the first position, the fourth liquid tank B4 connects the second flow channel port C2 and the third flow channel port C3, thereby connecting the first flow channel P1 and the second flow channel P2. The third flow channel P3 and the fourth flow channel P4 are not connected to other flow channels and are independent.

[0095] In the first position, the fluid is injected through the first flow channel port C1, and after passing through the first flow channel P1, the fourth liquid tank B4, the first section of the second flow channel P231, the sensing area A1 and the second section of the second flow channel P232, it is diverted into the first branch of the second flow channel P21 and the second branch of the second flow channel P22.

[0096] Please see Figure 5c When the flow channel switching component 120 rotates to the second position, the first flow channel B1 connects the fifth flow channel port C5 and the sixth flow channel port C6, and the third flow channel B3 connects the second flow channel port C2 and the third flow channel port C3, thereby connecting the first flow channel P1, the second flow channel P2 and the third flow channel P3 in sequence. The fourth flow channel P4 is not connected to other flow channels and is independent.

[0097] As can be seen, in the second position, the second flow channel P2 is connected to the third flow channel P3 through the second branch of the second flow channel P22. After the fluid is injected through the first flow channel port C1, it flows into the third flow channel P3 via the first flow channel P1, the third liquid passage B3, the first section of the second flow channel P231, the sensing area A1, the second section of the second flow channel P232, the second branch of the second flow channel P22, and the first liquid passage B1. In this way, the fluid is prevented from passing through the first branch of the second flow channel P21.

[0098] Please see Figure 5d When the flow channel switching component 120 rotates to the third position, the second liquid tank B2 connects the seventh flow channel port C7 and the ninth flow channel port C9, thereby connecting the third flow channel P3 and the fourth flow channel P4. The first flow channel P1 and the second flow channel P2 are not connected to other flow channels, and the first flow channel P1 and the second flow channel P2 are independent.

[0099] Please see Figure 5e When the flow channel switching component 120 rotates to the fourth position, the second flow channel B2 connects the fourth flow channel port C4 and the sixth flow channel port C6, and the fifth flow channel B5 connects the second flow channel port C2 and the third flow channel port C3, thereby connecting the first flow channel P1, the second flow channel P2 and the third flow channel P3 in sequence. The fourth flow channel P4 is not connected to other flow channels and is independent.

[0100] As can be seen, in the fourth position, the second flow channel P2 is connected to the third flow channel P3 through the first branch of the second flow channel P21. After the fluid is injected through the first flow channel port C1, it flows into the third flow channel P3 via the first flow channel P1, the fifth liquid passage B5, the first section of the second flow channel P231, the sensing area A1, the second section of the second flow channel P232, the first branch of the second flow channel P21, and the second liquid passage B2. This avoids the fluid from passing through the second branch of the second flow channel P22.

[0101] As described above, when the flow channel switching component 120 is in the first position, the fluid flows into the first branch P21 and the second branch P22 of the second flow channel via the main flow channel P23; when the flow channel switching component 120 is in the second position, the fluid flows to the second branch P22 of the second flow channel via the main flow channel P23; and when the flow channel switching component 120 is in the fourth position, the fluid flows to the first branch P21 of the second flow channel via the main flow channel P23. Therefore, by rotating the flow channel switching component 120 to different specific positions, the flow direction of the fluid entering the second flow channel P2 can be changed.

[0102] As can be seen, when the injected fluid does not need to interact with the pre-sealed fluid in the sealed chamber R, i.e., the injected fluid should not pass through the flow channel buffer A2, the flow channel switching component 120 is rotated to the second position. The fluid introduced into the flow channel flows to the second branch P22 of the second flow channel after passing through the sensing area A1. When the injected fluid needs to form a signal with the pre-sealed fluid in the sealed chamber R, the flow channel switching component 120 is rotated to the fourth position. The fluid introduced into the flow channel flows to the first branch P21 of the second flow channel after passing through the sensing area A1, thereby passing through the flow channel buffer A2 and exchanging ions with the fluid in the sealed chamber R, thus forming a detectable signal. Therefore, in this disclosure, by setting the second flow channel P2 as a bifurcated flow channel structure and cooperating with the flow channel switching component 120 to allow the fluid in the second flow channel P2 to flow to different branches, it adapts to the needs of different biochemical reaction stages, resulting in better versatility and a wider range of applications.

[0103] In this disclosure, the third flow channel P3 is a fluid collection area used to collect fluid. Its structure can vary; for example, the third flow channel P3 may include a reservoir for storing fluid, or it may be equipped with an absorbent medium, such as absorbent cotton. In a preferred embodiment, the third flow channel P3 is a meandering flow channel. In the illustrated embodiment, the third flow channel P3 has a serpentine structure, but it is not limited to this; for example, it could also be a spiral structure.

[0104] It should be noted that in this disclosure, the microfluidic detection device 1000 is provided with a first mode and a second mode. Different flow channels are connected by rotating the flow channel switching component 120, thereby allowing the microfluidic detection device 1000 to switch between the first mode and the second mode. When the flow channel switching component 120 is in the first or second position, the microfluidic detection device 1000 is in the first mode. When the flow channel switching component 120 is in the third or fourth position, the microfluidic detection device 1000 is in the second mode. In practical applications, depending on the application scenario, the first mode is the administrator mode, and the second mode is the user mode.

[0105] In the first mode, the fluid is used by the manufacturer for pre-processing operations. Specifically, when the flow channel switching component 120 is in the first position, it is used to pre-encapsulate fluid in the microcavity structure on the sensing chip 220. When the flow channel switching component 120 is in the second position, the injected fluid will not contact the membrane 140. For example, oil can be injected in the second position, and the oil will not wet the membrane 140, thus not affecting the permeation performance of the membrane 140.

[0106] In the second mode, when the flow channel switching component 120 is in the fourth position, it is used for user injection or aspiration operations, and the injected fluid will always pass through the membrane 140. When the flow channel switching component 120 is in the third position, it is used for user aspiration.

[0107] As described above, when the flow channel switching component 120 is in the second or fourth position, the fluid passing through the sensing area A1 flows into the third flow channel P3 for temporary storage. Understandably, the fluid in the third flow channel P3 can be extracted through the eighth flow channel port C8. However, this operation would cause air to be drawn into the sensing area A1 from the first flow channel port C1, thus forming air bubbles in the sensing area A1 and affecting the detection results. In practical applications, the flow channel switching component 120 is switched to the third position before liquid extraction is performed through the eighth flow channel port C8 or the tenth flow channel port C10. This isolates the second flow channel P2, preventing air from being drawn into the sensing area A1.

[0108] Please see Figures 5b to 5e The second flow channel port C2 to the seventh flow channel port C7 and the ninth flow channel port C9 are transition flow channel ports, designed to connect different flow channels in conjunction with different liquid transfer tanks. To ensure proper connection between the liquid transfer tank and the transition flow channel ports, the vertical projection of the flow channel switching component 120 covers the second flow channel port C2 to the seventh flow channel port C7 and the ninth flow channel port C9. In this disclosure, the liquid transfer tank is a long, narrow channel, but it is not limited to this; the key is to ensure that the liquid transfer tank and the transition flow channel ports fit together without leakage.

[0109] In this disclosure, the first flow channel C1, the eighth flow channel C8, and the tenth flow channel C10 are used for liquid injection or liquid extraction. The first flow channel C1, the eighth flow channel C8, and the tenth flow channel C10 can be located on the same side or different sides of the flow channel switching component 120, as long as they are not within the vertical projection coverage area of ​​the flow channel switching component 120. It should be noted that the shape of the flow channel orifice is not limited and can be adjusted according to actual needs, such as a round or square or flared orifice.

[0110] Figure 2b for Figure 2a One of the exploded views of the microfluidic device 100 in the image. See also... Figure 2b In this disclosure, the microfluidic device 100 further includes a fluid storage component 130, which is connected to the body 110 and communicates with a first flow channel C1. The fluid storage component 130 has a reservoir for temporarily storing fluid that needs to be injected into the body 110. In an optional embodiment, the reservoir in the fluid storage component 130 is funnel-shaped, wider at the top and narrower at the bottom, so that the fluid in the reservoir is concentrated towards the first flow channel C1. The fluid storage component 130 can be fixedly connected to the body 110; preferably, the fluid storage component 130 is detachably connected to the body 110, thus facilitating removal when the fluid storage component 130 is not needed.

[0111] As described above, the flow channel switching assembly 120 can adopt a single-layer plate structure to achieve switching and connection of each flow channel. In this way, all the liquid passing tanks will be concentrated on the single-layer sealing plate. Accordingly, the surface area of ​​the sealing plate must be large enough to meet the arrangement requirements of the liquid passing tanks, thus making the surface area of ​​the sealing plate covering body 110 larger and correspondingly occupying more space. In order to make the flow channel switching assembly 120 more compact and occupy less space, in this disclosure, the flow channel switching assembly 120 adopts a double-layer sealing plate structure.

[0112] Figure 2c for Figure 2a Exploded view of the microfluidic device 100, Part 2. Figure 2d for Figure 2c Exploded view three of the microfluidic device 100. Please refer to [the diagram]. Figure 2c and Figure 2d The flow channel switching assembly 120 includes an upper sealing plate 123 and a lower sealing plate 125. The first liquid passage B1 and the second liquid passage B2 are upper liquid passages and are disposed on the upper sealing plate 123. The third liquid passage B3, the fourth liquid passage B4 and the fifth liquid passage B5 are lower liquid passages and are disposed on the lower sealing plate 125.

[0113] Figure 2e for Figure 2c A magnified view of part Q in the image. Figure 2f for Figure 2d A magnified view of section X in the image. Please refer to [link / reference]. Figure 2e The fourth flow channel C4 to the seventh flow channel C7 and the ninth flow channel C9 are located on the upper surface of the body 110; please refer to Figure 2f The second flow channel C2 and the third flow channel C3 are located on the lower surface of the body 110.

[0114] As described above, the upper liquid-passing tank cooperates with the flow channel opening located on the upper surface of the body 110 to connect the third flow channel P3 with any one of the first branch of the second flow channel P21, the second branch of the second flow channel P22, and the fourth flow channel P4. The lower liquid-passing tank cooperates with the flow channel opening located on the lower surface of the body 110 to connect the first flow channel P1 with the second flow channel P2. It can be seen that by rotating the flow channel switching component 120 between the first and fourth positions, the positions of the upper and lower liquid-passing tanks are adjusted so that at least two of the multiple flow channels are connected via the upper liquid-passing tank and / or the lower liquid-passing tank.

[0115] As can be seen, in this disclosure, the flow channel switching component 120 adopts a double-layer sealing plate structure to achieve the connection of the switching flow channels. Compared with the single-layer sealing plate structure, the double-layer stacked layout structure occupies less space, is more compact, facilitates miniaturization design, and is easy to operate.

[0116] Understandably, to prevent fluid leakage, the upper sealing plate 123 and the lower sealing plate 125 are respectively attached to the upper and lower sides of the body 110. Secondly, the fourth flow channel C4 to the seventh flow channel C7 and the ninth flow channel C9 are overlapped with the upper sealing plate 123 in the vertical direction, and the second flow channel C2 and the third flow channel C3 are overlapped with the lower sealing plate 125 in the vertical direction. This ensures that the upper and lower liquid channels can be rotated to the corresponding flow channels.

[0117] In this disclosure, the main body 110 is provided with a limiting structure, and the flow channel switching assembly 120 includes a limiting component 124. During the rotation of the flow channel switching assembly 120 relative to the main body 110, the limiting component 124 also rotates together. The stopping position of the limiting component 124 is determined by the limiting structure so that the flow channel switching assembly 120 switches between the first position, the second position, the third position and the fourth position.

[0118] Please see Figures 2c to 2f The limiting structure includes a limiting groove and a sliding groove V3. The limiting component 124 can be a pin or a pre-compression spring pin, etc. The bottom end of the limiting component 124 can be embedded in the limiting groove to determine the relative position of the flow channel switching component 120 and the body 110. Secondly, the bottom end of the limiting component 124 can be embedded in the sliding groove V3 and slide within the sliding groove V3 to limit the rotation angle range of the flow channel switching component 120 relative to the body 110.

[0119] Please see Figure 2e The limiting groove includes a first limiting groove V1 and a second limiting groove V2. When the limiting component 124 switches to the first limiting groove V1, the flow channel switching component 120 is located in the first position; when the limiting component 124 switches to the second limiting groove V2, the flow channel switching component 120 is located in the second position.

[0120] In this disclosure, the limiting member 124 can move from the limiting groove into the slide groove V3 and slide along the slide groove V3, thereby allowing the flow channel switching assembly 120 to switch between the third position and the fourth position. Understandably, since the flow channel switching assembly 120 performs a rotary motion, the movement trajectory of the limiting member 124 is a circular trajectory, and correspondingly, the slide groove V3 is an arc-shaped groove.

[0121] In this disclosure, the limiting position is achieved by the structure of the slide groove V3 itself. When the limiting component 124 slides to the end of the slide groove V3 near the second limiting groove V2, the flow channel switching component 120 is in the third position. When the limiting component slides to the end of the slide groove V3 near the first limiting groove V1, the flow channel switching component 120 is in the fourth position.

[0122] In one optional embodiment, the slide V3 is formed by splicing two slide segments. Specifically, the slide V3 includes a first slide segment and a second slide segment. The limiting member 124 is configured to slide along the first slide segment to rotate the flow channel switching component 120 to a third position, and the limiting member 124 is configured to slide along the second slide segment to rotate the flow channel switching component 120 to a fourth position.

[0123] For example, the slide section near the second limiting groove V2 is the first slide section, and the slide section near the first limiting groove V1 is the second slide section. The limiting component 124 is a pre-compression spring pin. The bottom wall of the first slide section and the second slide section are respectively provided with a shallow pit. The bottom end of the pre-compression spring pin can pop out and embed itself in the shallow pit, generating a sense of lag during the rotation of the flow channel switching component 120, so that the user can determine that the flow channel switching component 120 is in the third or fourth position. Of course, when the rotation torque is increased, it is necessary to ensure that the pre-compression spring pin can be moved out of the shallow pit.

[0124] In a preferred embodiment, the depth of the groove V3 is greater than the depths of the first limiting groove V1 and the second limiting groove V2. Taking the limiting component 124 as an example of a pre-compression spring, when the groove depth of the groove V3 is sufficiently large, the pre-compression spring extends a long length when it is located in the groove V3, making it impossible for the pre-compression spring to retract and thus preventing it from being removed from the groove V3. Therefore, when the bottom end of the limiting component 124 is in the groove V3, the flow channel switching component 120 can only switch between the third and fourth positions. In other words, after the flow channel switching component 120 switches from the first or second position to the groove V3, it cannot switch back to the first or second position. As mentioned above, in this disclosure, the microfluidic detection device 100 has a first mode and a second mode. By reasonably designing the limiting structure, the microfluidic detection device 100 cannot switch back from the second mode to the first mode, thereby preventing user misoperation.

[0125] As can be seen, in this disclosure, the flow channel switching component 120 is essentially a five-position valve, which, in conjunction with the limiting structure, allows for four-position switching to connect and disconnect different flow channels, thereby controlling the fluid flow direction.

[0126] Figure 3a for Figure 2a A schematic diagram of the flow channel switching component 120 in the middle. Figure 3b for Figure 3a An exploded view of the flow channel switching component 120. See also... Figure 2c , Figure 2d , Figure 3a and Figure 3bThe flow channel switching assembly 20 also includes a rotating body 121 and a rotating shaft 122. The body 110 is provided with a rotating shaft mounting hole, and the rotating shaft 122 can be inserted into the rotating shaft mounting hole. The rotating shaft 122 and the rotating shaft mounting hole are in clearance fit, so that the rotating shaft 122 can rotate in the rotating shaft mounting hole.

[0127] Secondly, the two ends of the rotating shaft 122 are respectively connected to the rotating body 121 and the lower sealing plate 125, and the upper sealing plate 123 and the limiting component 124 are connected to the rotating body 121. Since the upper sealing plate 123 is in contact with the body 110, the upper sealing plate 123 is located between the rotating body 121 and the body 110. It can be understood that the rotating body 121 is rotatably connected to the body 110 through the rotating shaft 122. During the process of twisting the rotating body 121, the upper sealing plate 123, the limiting component 124 and the lower sealing plate 123 rotate accordingly.

[0128] In this disclosure, the rotating body 121 is provided with a shaft insertion hole K, into which the top end of the rotating shaft 122 can be inserted. To reduce the risk of relative rotation between the rotating shaft 122 and the rotating body 121, preferably, the shaft insertion hole K is an oblong hole, and correspondingly, the radial cross-section of the top end of the rotating shaft 122 is oblong, so that it can be inserted into the shaft insertion hole K. Of course, the shaft insertion hole K can also be other shaped holes, such as rectangular holes, triangular holes, or other non-circular holes, and no specific limitation is made here.

[0129] In one alternative embodiment, the top end of the rotating shaft 122 and the rotating shaft insertion hole K can be interference-fitted, thereby achieving a fixed connection between the rotating shaft 122 and the rotating body 121. In this disclosure, the top end of the rotating shaft 122 is fixedly connected to the rotating body 121 by a fastening connector (e.g., screw, pin).

[0130] In this disclosure, a tray 1221 is provided at the bottom end of the rotating shaft 122, and a lower sealing plate 125 is connected to the tray 1221. Specifically, the lower sealing plate 125 can be placed onto the tray 1221 from the top of the rotating shaft 122, and the tray 1221 supports the lower sealing plate 125. It can be understood that since the lower sealing plate 125 needs to fit against the body 110, the lower sealing plate 125 is located between the body 110 and the tray 1221. It should be noted that the upper sealing plate 123 and the rotating body 121, as well as the lower sealing plate 125 and the tray 1221, can be connected by means of hot melting, ultrasonic welding, laser welding, adhesive bonding, etc.

[0131] In this disclosure, the projection of the upper sealing plate 123 in the vertical direction at least covers the lower sealing plate 125. As mentioned above, the sensing device 200 is connected to the lower part of the body 110. In order to leave more space for the installation of the sensing device 200, the lower sealing plate 125 is made as small as possible while satisfying the liquid passage layout. Although the number of liquid passages arranged on the lower sealing plate 125 is greater than the number of liquid passages on the upper sealing plate 123, the area of ​​the lower sealing plate 125 is still smaller than the area of ​​the upper sealing plate 123.

[0132] Figure 4a This is a partial exploded view of a microfluidic device 100 provided according to one embodiment of the present disclosure. Figure 4b for Figure 4a Another exploded view of the microfluidic device 100 in the image; Figure 4c for Figure 4b An exploded view of the microfluidic device 100 from another perspective. Figures 4a to 4c This disclosure primarily illustrates the structure of the body 110 and the formation of each flow channel and sealing chamber R. Please refer to [link / reference needed]. Figures 4a to 4c In this disclosure, the body 110 is a multi-plate assembly structure. Specifically, the body 110 includes an upper plate 111, a flow channel plate 112, and a lower plate 113. In specific applications, the plates can be assembled through processes such as bonding, ultrasonic welding, laser welding, or adhesive bonding.

[0133] Furthermore, flow channel plates 112 are provided with flow channel grooves on both their upper and lower surfaces. Lower plates 111 and 113 are respectively disposed on the upper and lower sides of the flow channel plate 112 to form multiple flow channels together with the flow channel grooves. Understandably, the multiple flow channels arranged on the upper and lower sides of the flow channel plate 112 form a double-layer flow channel structure, which greatly reduces the space occupied by the microfluidic device 100 and achieves the purpose of miniaturization.

[0134] Specifically, the flow channel includes a first flow channel G1, a second flow channel G2, a third flow channel G3, and a fourth flow channel G4, for forming the first flow channel P1, the second flow channel P2, the third flow channel P3, and the fourth flow channel P4, respectively.

[0135] Please see Figures 4a to 4c In this disclosure, the second flow channel C2 and the third flow channel C3 are disposed on the lower plate 113, and the first flow channel C1 and the fourth flow channel C4 to the tenth flow channel C10 are disposed on the upper plate 111.

[0136] In this disclosure, the first flow channel G1 includes a first flow channel first section G11 and a first flow channel second section G12. The first flow channel first section G11 is located on the upper surface of the flow channel plate 112, and the first flow channel second section G12 is located on the lower surface of the flow channel plate 112. The first flow channel opening C1 connects the first flow channel first section G11, and the second flow channel opening C2 connects the first flow channel second section G12. The flow channel plate 112 is provided with a first through hole H1. The first flow channel first section G11 and the first flow channel second section G12 are connected by the first through hole H1 to form the first flow channel G1. When the upper plate 111 and the lower plate 113 cover the flow channel plate 112, they together form the first flow channel P1.

[0137] In this disclosure, the third flow channel G3 includes a first section G31, a second section G32, and a third section G33. The first section G31 and the third section G33 are located on the upper surface of the flow channel plate 112, and the second section G32 is located on the lower surface of the flow channel plate 112. The sixth flow channel port C6 and the seventh flow channel port C7 are both connected to the first section G31, and the eighth flow channel port C8 is connected to the third section G33.

[0138] The flow channel plate 112 is also provided with a third through hole H3 and a fourth through hole H4. The third through hole H3 and the fourth through hole H4 are located at both ends of the second section G32 of the third flow channel, thereby connecting the first section G31, the second section G32 and the third section G33 of the third flow channel in sequence to form the third flow channel G3. When the upper plate 111 and the lower plate 113 cover the flow channel plate 112, they together form the third flow channel P3.

[0139] As mentioned above, in one optional embodiment, the third flow channel P3 is a meandering flow channel to facilitate fluid collection. Understandably, the third flow channel P3 occupies a large space, therefore, the third flow channel P3 is arranged on the upper and lower sides of the flow channel plate 112, so that the third flow channel P3 has a stacked arrangement structure, which is beneficial for miniaturization.

[0140] In this disclosure, the fourth flow channel G4 is a single flow channel segment located on the upper surface of the flow channel plate 112. The ninth flow channel port C9 and the tenth flow channel port C10 are both connected to the fourth flow channel G4, and together they form the fourth flow channel P4 when the upper plate 111 covers the flow channel plate 112.

[0141] In this disclosure, the second flow channel G2 includes a first section G21, a second section G22, and a third section G23. The first section G21 and the second section G22 are located on the lower surface of the flow channel plate 112, and the third section G23 is located on the upper surface of the flow channel plate 112. The third flow channel opening C3 is connected to the first section G21.

[0142] The first liquid passage D1 and the second liquid passage D2 are located on the lower plate 113. The flow channel plate 112 is also provided with a second through hole H2. The second through hole H2 is located at the end of the second section G22 of the second flow channel, so as to connect the second section G22 of the second flow channel with the third section G23 of the second flow channel. When the upper plate 111 and the lower plate 113 cover the flow channel plate 112, they together form the second section P232 of the second flow channel. The first section G21 of the second flow channel and the lower plate 113 cooperate together to form the first section P231 of the second flow channel.

[0143] Furthermore, the second flow channel G2 also includes a fourth section G24 and a fifth section G25 of the second flow channel. Both the fourth section G24 and the fifth section G25 of the second flow channel are located on the upper surface of the flow channel plate 112. The fourth flow channel opening C4 is connected to the fourth section G24 of the second flow channel, and the fifth flow channel opening C5 is connected to the fifth section G25 of the second flow channel.

[0144] The fourth section G24 and the fifth section G25 of the second flow channel are both connected to the third section G23 of the second flow channel and together with the upper plate 111, they form the first branch P21 and the second branch P22 of the second flow channel, respectively.

[0145] Furthermore, the first section G21 of the flow channel is provided with a first buffer zone G211; the second section G22 of the flow channel is provided with a second buffer zone G221; the shape of the first buffer zone G211 gradually expands from the side away from the first liquid outlet D1 towards the side closer to the first liquid outlet D1; the shape of the second buffer zone G221 gradually expands from the side away from the second liquid outlet D2 towards the side closer to the second liquid outlet D2. In other words, the channel width on the side closer to the liquid outlet is greater than the channel width on the side away from the liquid outlet, thus resulting in a lower fluid flow velocity at the sensing area A1 to facilitate sensing. In this disclosure, the first buffer zone G211 and the second buffer zone G221 are isosceles trapezoids, but are not limited to this; for example, they can be semicircles.

[0146] It should be noted that the number and arrangement of the channels in each flow channel can be adjusted as needed, and no specific restrictions are imposed here.

[0147] Please see Figures 4a to 4b In this disclosure, the lower surface of the flow channel plate 112 is provided with a liquid storage tank L, which is sealed by the membrane 140 and the lower plate 113 to form a sealed chamber R. In a specific application, the flow channel buffer zone A2 has a through opening formed on the bottom wall at the corresponding position of the fourth section G24 of the second flow channel to connect with the liquid storage tank L. The membrane 140 seals and fits the through opening. It can be seen that in this disclosure, the membrane 140 is located between the outlet tank L and the flow channel buffer zone A2 to separate the sealed chamber R from the fourth section G24 of the second flow channel, so that the fluid in the second section G22 of the second flow channel and the fluid in the sealed chamber R can only exchange ions.

[0148] In a specific application, the lower plate 113 is provided with an opening for mounting the conductive sheet 150. This opening overlaps with the liquid storage tank L in the vertical direction, that is, the opening is connected to the liquid outlet tank L and is sealed by the conductive sheet 150, so that both the conductive sheet 150 and the membrane 140 are wetted by the fluid in the sealed chamber R.

[0149] Please see Figure 4c The upper plate 111 is also provided with an eleventh flow channel port C11 and a twelfth flow channel port C12. The flow channel plate 112 is also provided with a fifth through hole H5 and a sixth through hole H6. The eleventh flow channel port C11 is connected to the liquid passage tank L through the fifth through hole H5, and the twelfth flow channel port C12 is connected to the liquid passage tank L through the sixth through hole H6. Both the eleventh flow channel port C11 and the twelfth flow channel port C12 are connected to the sealed chamber R. It can be understood that the detection liquid is injected into the sealed chamber R through one of the eleventh flow channel port C11 and the twelfth flow channel port C12, while the other is used to expel the air in the sealed chamber R. Only in this way can the detection liquid be smoothly injected into the sealed chamber. It can be seen that the eleventh flow channel port C11 and the twelfth flow channel port C12 are used for injecting the detection liquid.

[0150] Please see Figure 2b In this disclosure, the microfluidic device 100 also includes a sealing patch 160, which covers the eleventh flow channel port C11 and the twelfth flow channel port C12 to pre-seal the fluid in the sealed chamber R and prevent fluid leakage.

[0151] In this disclosure, the sealed chamber R is formed by the liquid storage tank L in conjunction with the membrane 140 and the lower plate 113. Of course, it is not limited to this. For example, the sealed chamber R is formed in a sealed container provided with the membrane 140 and the conductive sheet 150. The sealed container is embedded in the body 110, or even detachably connected to the body 110. It can be seen that the structure and shape of the sealed chamber R can be diverse, and no specific limitation is made here.

[0152] It should be noted that in this disclosure, the body 110 is a three-plate-like structure stacked and assembled vertically, but it is not limited to this. For example, in one optional embodiment, the body 110, which has various flow channels and a liquid storage tank L, is manufactured using an integral molding process, such as 3D printing; then the membrane 140 and the conductive sheet 150 are assembled to seal the liquid storage tank L to form a sealed chamber R, followed by the assembly of the flow channel switching component 120, the fluid storage component 130, and the sealing patch 160. Understandably, this method has a higher production cost, especially since the membrane 140 is difficult to assemble. Alternatively, in another optional embodiment, the flow channel plate 112 and the upper plate 111 or the lower plate 113 are manufactured using an integral molding process, and the body 110 is made using a two-plate assembly structure, thus avoiding the difficulty of assembling the membrane 140, but the production cost is higher. It can be seen that the structure of the body 110 is not limited to this embodiment, and no specific limitations are made here.

[0153] In practical applications, the observation area A3 has openings in the projection areas of the upper plate 111 and the flow channel plate 112, and the projection area of ​​the lower plate 113 is set as a transparent area, or the entire lower plate 113 is made of transparent material (such as a transparent acrylic plate), thus forming the observation area A3 for convenient viewing of the sensing area A1.

[0154] It should be noted that in this disclosure, the slide V3 is a quarter-circular arc groove, and the two ends of the first limiting hole V1, the second limiting hole V2 and the slide V3 are evenly spaced relative to the rotation center of the flow channel switching assembly 120, so that the included angle between the line connecting two adjacent special positions and the rotation center is 90°.

[0155] As previously stated, when the flow channel switching component 120 is in the first position, the limiting component 124 is located at the first limiting groove V1. Figure 6 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid passage groove, respectively, when the limiting component 124 is located in the first limiting groove V1.

[0156] Please see Figure 6 The previous liquid tanks did not connect the different flow channels. The fourth liquid tank B4 connects the second section G12 of the first flow channel and the first section G21 of the second flow channel. Thus, when fluid is injected through the first flow channel port C1, the fluid sequentially passes through the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the fourth liquid tank B4, the first section G21 of the second flow channel, and the sensing area A1. Figure 6 (Not shown), the second section G22 of the second flow channel, the second through hole H2 and the third section G23 of the second flow channel, then the flow is diverted into the fourth section G24 and the fifth section G25 of the second flow channel.

[0157] The flow channel switching assembly 120 rotates 90° counterclockwise from the first position, and the flow channel switching assembly 120 switches to the second position, with the limiting component 124 in the second groove V2. Figure 7 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid passage groove, respectively, when the limiting component 124 is located in the second limiting groove V2.

[0158] Please see Figure 7 The first liquid-passing tank B1 connects the fifth section G25 of the second flow channel to the first section G31 of the third flow channel, and the third liquid-passing tank B3 connects the second section G12 of the first flow channel and the first section G21 of the second flow channel. Thus, fluid is injected through the first flow channel opening C1, and the fluid sequentially passes through the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the third liquid-passing tank B3, the first section G21 of the second flow channel, and the sensing area A1. Figure 7 (Not shown) After passing through the second section G22 of the second flow channel, the second through hole H2, the third section G23 of the second flow channel, the fifth section G25 of the second flow channel, and the first liquid passage B1, it enters the first section G31 of the third flow channel, enters through the third through hole H3 and gradually fills the second section G32 of the third flow channel, and then enters the third section G33 of the third flow channel through the fourth through hole H4.

[0159] The flow channel switching assembly 120 rotates 90° counterclockwise from the second position, and the flow channel switching assembly 120 switches to the third position, with the limiting component 124 located at one end of the slide groove V3 near the second limiting groove V2. Figure 8 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid passage groove, respectively, when the limiting component 124 is located at one end of the slide groove V3 near the second limiting groove V2.

[0160] Please see Figure 8 The lower liquid tank does not connect the different flow channels. The second liquid tank B2 connects the first section of the third flow channel G31 with the fourth flow channel G4. It should be noted that in specific applications, the eighth flow channel port C8 is a waste discharge port, through which the fluid temporarily stored in the third flow channel G3 is extracted. The tenth flow channel port C10 is a balance port used to connect to the atmosphere during liquid extraction. When waste liquid is extracted through the eighth flow channel port C8, the fluid flows along the first section of the third flow channel G31, the third through hole H3, the second section of the third flow channel G32, the fourth through hole H4, and the third section of the third flow channel G33, and flows out from the eighth flow channel port C8.

[0161] The flow channel switching component 120 rotates 90° counterclockwise from the third position, and the flow channel switching component 120 switches to the fourth position, with the limiting component 124 located at the end of the slide groove V3 near the first limiting groove V1. Figure 9 Figures (a) and (b) show the relative positional relationship between the flow channel groove on the upper surface of the flow channel plate 112 and the upper liquid passage groove, and the relative positional relationship between the flow channel groove on the lower surface of the flow channel plate 112 and the lower liquid passage groove, respectively, when the limiting component 124 is located at one end of the slide groove V3 near the first limiting groove V1.

[0162] Please see Figure 9 The second liquid-passing tank B2 connects the fourth section G24 of the second flow channel to the first section G31 of the third flow channel, and the fifth liquid-passing tank B5 connects the second section G12 of the first flow channel and the first section G21 of the second flow channel. Thus, fluid is injected through the first flow channel opening C1, and the fluid sequentially passes through the first section G11 of the first flow channel, the first through hole H1, the second section G12 of the first flow channel, the fifth liquid-passing tank B5, the first section G21 of the second flow channel, and the sensing area A1. Figure 9 (Not shown) After passing through the second section G22 of the second flow channel, the second through hole H2, the third section G23 of the second flow channel, the fourth section G24 of the second flow channel (including the flow channel buffer zone A2) and the second liquid passage B2, it enters the first section G31 of the third flow channel, enters through the third through hole H3 and gradually fills the second section G32 of the third flow channel, and then enters the third section G33 of the third flow channel through the fourth through hole H4.

[0163] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0164] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0165] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A microfluidic device, characterized in that, The microfluidic device includes a body, a flow channel switching component, and a membrane; The main body is provided with a second flow channel and a sealed chamber. The second flow channel includes a first branch of the second flow channel, a second branch of the second flow channel, and a main channel of the second flow channel. The first branch of the second flow channel and the second branch of the second flow channel converge at the main channel of the second flow channel. The sealed chamber is located below the first branch of the second flow channel. The membrane is disposed between the sealed chamber and the first branch of the second flow channel. The membrane is configured to allow ions to pass through, and the fluid in the sealed chamber and the fluid in the first branch of the second flow channel can exchange ions. The flow channel switching component is rotatably connected to the body to direct the fluid in the main flow channel of the second flow channel to the first branch of the second flow channel and / or the second branch of the second flow channel.

2. The microfluidic device according to claim 1, characterized in that, The body is provided with multiple flow channels, including a third flow channel and a second flow channel; The flow channel switching component is provided with a first liquid passage tank and a second liquid passage tank; When the flow channel switching component is rotated to the second position, the first liquid passage is connected to the second branch of the second flow channel and the third flow channel; When the flow channel switching component is rotated to the fourth position, the second liquid passage is connected to the first branch of the second flow channel and the third flow channel.

3. The microfluidic device according to claim 2, characterized in that, The third flow channel is a meandering flow channel used to collect fluid.

4. The microfluidic device according to claim 3, characterized in that, The third flow channel includes an eighth flow channel port, which is located on one side of the flow channel switching assembly and is used to discharge fluid from the third flow channel.

5. The microfluidic device according to claim 2, characterized in that, The plurality of flow channels also includes a fourth flow channel, and the flow channel switching component is configured such that when rotated to the third position, the second liquid passage connects the third flow channel and the fourth flow channel.

6. The microfluidic device according to claim 5, characterized in that, The fourth flow channel includes a tenth flow channel opening, which is located on one side of the flow channel switching component.

7. The microfluidic device according to claim 5, characterized in that, The plurality of flow channels also includes a first flow channel; The flow channel switching component is also provided with a third liquid passage tank, a fourth liquid passage tank and a fifth liquid passage tank; When the flow channel switching component is rotated to the first position, the fourth liquid passage tank connects the first flow channel and the main flow channel of the second flow channel. When the flow channel switching component is rotated to the second position, the third liquid passage tank is connected to the main flow path of the first flow channel and the second flow channel. When the flow channel switching component is rotated to the fourth position, the fifth liquid passage connects the first flow channel and the main flow channel of the second flow channel.

8. The microfluidic device according to claim 7, characterized in that, The first flow channel includes a first flow channel opening, which is located on one side of the flow channel switching component; The microfluidic device further includes a fluid storage component, which is connected to the main body and communicates with the first flow channel.

9. The microfluidic device according to claim 1, characterized in that, The first branch of the second flow channel is provided with a flow channel buffer, which is gradually expanded and then gradually narrowed along the extension direction of the first branch of the second flow channel; The sealed chamber is located below the flow channel buffer zone, and the membrane is located between the sealed chamber and the flow channel buffer zone.

10. The microfluidic device according to claim 1, characterized in that, The body is provided with multiple flow channels, including the second flow channel; The body includes an upper plate, a flow channel plate, and a lower plate, and the upper and lower surfaces of the flow channel plate are provided with flow channel grooves. The upper plate and the lower plate are respectively disposed on the upper and lower sides of the flow channel plate to form a plurality of flow channels together with the flow channel groove.

11. The microfluidic device according to claim 10, characterized in that, The plurality of flow channels also includes a first flow channel; The flow channel includes a first flow channel, which includes a first flow channel first section and a first flow channel second section; The first section of the first flow channel groove is connected to the second section of the first flow channel groove and is located on the upper surface and lower surface of the flow channel plate, respectively. The first flow channel groove, together with the upper plate and the lower plate, forms the first flow channel.

12. The microfluidic device according to claim 10, characterized in that, The plurality of flow channels also includes a third flow channel; The flow channel includes a third flow channel, which includes a first section of the third flow channel, a second section of the third flow channel, and a third section of the third flow channel. The first section and the third section of the third flow channel are located on the upper surface of the flow channel plate, and the second section of the third flow channel is located on the lower surface of the flow channel plate. The first section, the second section, and the third section of the third flow channel are sequentially connected and together with the upper plate and the lower plate form the third flow channel.

13. The microfluidic device according to claim 10, characterized in that, The plurality of flow channels also includes a fourth flow channel; The flow channel includes a fourth flow channel, which is located on the upper surface of the flow channel plate and forms the fourth flow channel together with the upper plate.

14. The microfluidic device according to claim 10, characterized in that, The second flow channel main road includes the first section of the second flow channel and the second section of the second flow channel; The flow channel includes a second flow channel, which includes a first section of the second flow channel, a second section of the second flow channel, and a third section of the second flow channel. The first and second sections of the second flow channel are located on the lower surface of the flow channel plate, and the third section of the second flow channel is located on the upper surface of the flow channel plate. The first section of the second flow channel groove together with the lower plate forms the first section of the second flow channel, and the second section of the second flow channel groove is connected to the third section of the second flow channel groove and together with the upper plate and the lower plate forms the second section of the second flow channel; The first section of the second flow channel includes a first liquid outlet, and the second section of the second flow channel includes a second liquid outlet. The first liquid outlet and the second liquid outlet are arranged at intervals on the lower plate, and the first section of the second flow channel and the second section of the second flow channel can be connected through the first liquid outlet and the second liquid outlet.

15. The microfluidic device according to claim 14, characterized in that, The second flow channel also includes a fourth section and a fifth section of the second flow channel, which are located on the upper surface of the flow channel plate. The fourth section of the second flow channel is connected to the third section of the second flow channel and forms the first branch of the second flow channel with the upper plate; The fifth section of the second flow channel is connected to the third section of the second flow channel and forms the second branch of the second flow channel with the upper plate.

16. The microfluidic device according to claim 14, characterized in that, The first section of the second flow channel is provided with a first buffer zone; the second section of the second flow channel is provided with a second buffer zone; The shape of the first buffer zone gradually expands from the side away from the first liquid outlet to the side closer to the first liquid outlet; The shape of the second buffer zone gradually expands from the side away from the second liquid outlet to the side closer to the second liquid outlet.

17. The microfluidic device according to claim 10, characterized in that, The lower surface of the flow channel plate is provided with a liquid storage tank; The liquid storage tank is sealed by a membrane and the lower plate, thereby forming the sealed chamber; The membrane is disposed between the liquid storage tank and the first branch of the second flow channel; The lower plate is provided with a conductive sheet at the position where it overlaps with the liquid storage tank in the vertical direction.

18. The microfluidic device according to claim 7, characterized in that, The flow channel switching assembly includes an upper sealing plate and a lower sealing plate. The upper sealing plate and the lower sealing plate are respectively attached to the upper and lower sides of the body. The first liquid passage groove and the second liquid passage groove are disposed on the upper sealing plate, and the third liquid passage groove, the fourth liquid passage groove and the fifth liquid passage groove are disposed on the lower sealing plate.

19. The microfluidic device according to claim 18, characterized in that, The first flow channel includes a second flow channel opening disposed on the lower surface of the body; The second main flow path includes a third flow path opening disposed on the lower surface of the body; The second flow channel and the third flow channel overlap with the lower sealing plate in the vertical direction.

20. The microfluidic device according to claim 18, characterized in that, The first branch of the second flow channel includes a fourth flow channel opening disposed on the upper surface of the body; The second branch of the second flow channel includes a fifth flow channel opening disposed on the upper surface of the body; The third flow channel includes a sixth flow channel opening and a seventh flow channel opening disposed on the upper surface of the body; The fourth flow channel includes a ninth flow channel opening disposed on the upper surface of the body; The fourth, fifth, sixth, seventh, and ninth flow channels overlap with the upper sealing plate in the vertical direction.

21. The microfluidic device according to claim 20, characterized in that, The main body is equipped with a limiting structure; The flow channel switching assembly further includes a limiting component. During the rotation of the flow channel switching assembly relative to the body, the stopping position of the limiting component is determined by the limiting structure, so that the flow channel switching assembly can switch between the first position, the second position, the third position, and the fourth position.

22. The microfluidic device according to claim 21, characterized in that, The limiting structure includes a first limiting groove, a second limiting groove, and a sliding groove; When the limiting component is switched to the first limiting groove, the flow channel switching component is located in the first position; when the limiting component is switched to the second limiting groove, the flow channel switching component is located in the second position. The limiting component is configured to slide along the groove to allow the flow channel switching component to switch between the third position and the fourth position.

23. The microfluidic device according to claim 22, characterized in that, The chute includes a first chute section and a second chute section; The limiting component is configured to slide along the first slide section to rotate the flow channel switching component to the third position; the limiting component is configured to slide along the second slide section to rotate the flow channel switching component to the fourth position.

24. The microfluidic device according to claim 22, characterized in that, The groove depth is greater than the groove depth of the first limiting groove and the second limiting groove.

25. The microfluidic device according to claim 22, characterized in that, The flow channel switching assembly further includes a rotating body and a rotating shaft. The rotating body is rotatably connected to the main body via the rotating shaft. The limiting component and the upper sealing plate are connected to the rotating body and can rotate with the rotating body. The two ends of the rotating shaft are respectively connected to the rotating body and the lower sealing plate, and the lower sealing plate can rotate with the rotating shaft.

26. A microfluidic detection device, characterized in that, The microfluidic detection device includes a sensing device and a microfluidic device according to any one of claims 1 to 25; The sensing device is disposed at the main channel of the second flow channel and connected to the body, and the sensing device and the body together form a sensing area in the corresponding part of the main channel of the second flow channel.

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