A transdermal migration device

Abstract

The present application relates to a kind of transdermal migration devices, including the receiving pool for containing human body fluid, adsorption phase, link cover, diffusion membrane, sealing element, diffusion cell for placing sample and top cover;Adsorption phase is placed in receiving pool;Link cover is recessed, hollow in the middle, and the upper and lower ends are respectively detachably connected to diffusion cell and receiving pool;Diffusion membrane is laid in the recess of link cover and is fixed by diffusion cell, and the upper and lower surfaces of diffusion membrane are respectively closely attached to sample in diffusion cell and human body fluid in receiving pool;Link cover is provided with air hole, for connecting receiving pool and ambient air, and sealing element is detachably blocked in air hole;Top cover covers the top of diffusion cell.The device is set through diffusion membrane, so that sample is only contacted with human body fluid through membrane surface, and realizes that chemical substance in sample is unidirectional migration to human body fluid;The position of diffusion membrane is fixed by the design of link cover, and it is convenient to place and replace adsorption phase.The device is simple to operate, and can resist environmental background anti-interference.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of risk assessment of human body exposure to consumer products, in particular to a transdermal migration device. BACKGROUND

[0002] Skin, as the largest organ of the human body, is exposed to the environment and numerous chemicals at all times. Among them, the skin contacting the surface of consumer products is a common skin exposure scenario. Chemicals in consumer products can enter the human body through the skin from the surface of consumer products. At present, the exposure level of chemicals in consumer products to human skin can be estimated by methods such as wiping the surface of consumer products or artificial immersion, to explore the migration rules of chemicals in consumer products mediated by sweat and assess the risk of human transdermal exposure. Among them, the wiping method is affected by the wiping force and wiping liquid, and can only evaluate the release potential in a very short time. The artificial sweat immersion method often cuts or completely breaks the consumer products for immersion. This method can effectively increase the contact area of consumer products and sweat and shorten the evaluation time. However, this method greatly destroys the structure of consumer products and ignores the actual situation that the skin only contacts the surface of consumer products.

[0003] Franz transdermal diffusion cell is often used for transdermal test of preparations such as patches, suppositories and semisolid preparations, which can objectively reflect the transdermal release process of the preparation. However, this device is often used for polar and high-solubility chemicals, and it is not easy to reach the detection limit of the instrument for low-solubility chemicals. Existing studies often use the method of adding adsorption phase, such as XAD resin, polydimethylsiloxane and polyethylene, to enrich low-solubility chemicals in environmental water samples, so as to improve the detection amount of target substances. However, the repeated replacement of adsorbents will greatly affect the position of the diffusion membrane in the Franz transdermal diffusion cell. In addition, the chemicals in consumer products include non-polar and low-solubility trace substances, which are easily contaminated by the potential environmental background. SUMMARY

[0004] In view of the problems in the prior art, the purpose of the present application is to provide a transdermal migration device, which is easy to operate, can resist environmental background interference, and can highly simulate the migration process of chemicals from the surface of consumer products to human body fluids through the skin.

[0005] In order to achieve the above purpose, the present application adopts the following technical scheme:

[0006] A transdermal migration device, comprising a receiving pool for containing human body fluids, an adsorption phase, a linking cover, a diffusion membrane, a sealing member, a diffusion cell for placing a sample, and a top cover;

[0007] The adsorption phase is placed in the receiving pool;

[0008] The linking cover is in the form of a groove, hollow in the middle, and detachably connected to the diffusion cell and the receiving pool at its upper and lower ends, respectively;

[0009] The diffusion membrane is laid in the groove of the linking cover and fixed by the diffusion cell, and the upper and lower surfaces of the diffusion membrane are tightly attached to the sample in the diffusion cell and the human body fluid in the receiving cell respectively;

[0010] The linking cover is provided with a vent hole for connecting the receiving cell with the ambient air, and the sealing member is detachably blocked in the vent hole.

[0011] The top cover covers the top of the diffusion cell.

[0012] Further, the linking cover is threadedly connected to the diffusion cell and the receiving cell respectively, and the diffusion membrane is fixed by threadedly rotating the linking cover with the diffusion cell.

[0013] Further, the lower peripheral surface of the linking cover is provided with external threads, and the upper peripheral surface of the receiving cell is provided with matching internal threads; the upper peripheral surface of the linking cover is provided with internal threads, and the lower peripheral surface of the diffusion cell is provided with matching external threads.

[0014] Further, a small sealing ring is arranged on the diffusion membrane, and the diffusion cell abuts against the small sealing ring.

[0015] Further, a large sealing ring is arranged at the joint of the linking cover and the receiving cell.

[0016] Further, the lower peripheral surface of the linking cover is provided with a ring groove, and the large sealing ring is sleeved in the ring groove.

[0017] Further, the vent hole is a screw hole, and the sealing member is a screw which is threadedly connected to the screw hole.

[0018] Further, the adsorption phase is made of XAD resin, polydimethylsiloxane or polyethylene.

[0019] Further, the diffusion membrane is made of dialysis membrane or skin membrane.

[0020] Further, the receiving cell, the linking cover, the diffusion cell and the top cover are made of stainless steel, glass or plastic.

[0021] In general, the present application has the following advantages:

[0022] The transdermal migration device of the present application aims to highly simulate the migration process of chemical substances from the surface of a consumer product to human body fluid through the skin.

[0023] (1) The diffusion membrane is arranged in the groove of the linking cover and fixed by threadedly rotating the linking cover with the diffusion cell, and the upper and lower surfaces of the diffusion membrane are tightly attached to the sample in the diffusion cell and the human body fluid in the receiving cell respectively, so that the sample can only be in full contact with the human body fluid through the surface of the diffusion membrane, and the one-way migration of chemical substances in the sample to the human body fluid is realized.

[0024] (2) The receiving cell, the connecting cover and the diffusion cell can be joined together by screwing them together; the joining can be undone by simply screwing the receiving cell and the connecting cover together, without moving the diffusion membrane, which facilitates the recovery and replacement of the adsorbed phase.

[0025] (3) The vent hole of the connecting cover can easily discharge the gas in the receiving pool and extract the human body fluid in the receiving pool. The vent hole can be sealed by the sealing element to cut off the connection between the receiving pool and the external atmosphere of the device, thus ensuring the airtightness of the device.

[0026] (4) The device is fully enclosed after assembly, which can prevent the human body fluid in the receiving pool from being reduced due to leakage or evaporation. At the same time, it can resist environmental background interference and improve the accuracy of detection and analysis. Attached Figure Description

[0027] Figure 1 A schematic diagram of the transdermal migration device according to the present invention is shown.

[0028] In the picture:

[0029] 1 is the receiving cell; 2 is the adsorption phase; 3 is the large sealing ring; 4 is the connecting cover; 401 is the groove; 402 is the annular groove; 403 is the screw hole; 5 is the diffusion membrane; 6 is the small sealing ring; 7 is the screw; 8 is the diffusion cell; 9 is the top cover. Detailed Implementation

[0030] The present invention will now be described in further detail.

[0031] like Figure 1 As shown, a transdermal migration device includes a receiving cell 1, an adsorption phase 2, a large sealing ring 3, a connecting cover 4, a diffusion membrane 5, a small sealing ring 6, a screw 7, a diffusion cell 8, and a top cover 9.

[0032] The receiving cell 1 is used to hold human body fluids, and the connecting cover 4 is used to connect the receiving cell 1 and the diffusion cell 8. The diffusion cell 8 is used to place the sample.

[0033] The connecting cover 4 is detachably connected to the diffusion pool 8 and the receiving pool 1, preferably by a threaded connection. Specifically, the lower circumferential surface of the connecting cover 4 is provided with an external thread, and the upper circumferential surface of the receiving pool 1 is provided with a matching internal thread; the upper circumferential surface of the connecting cover 4 is provided with an internal thread, and the lower circumferential surface of the diffusion pool 8 is provided with a matching external thread.

[0034] The connecting cover 4 is shaped like a groove 401 with a hollow center, an annular groove 402 on the lower circumference, and two symmetrically distributed vent holes on the top, preferably screw holes 403.

[0035] Screw hole 403 connects the receiving pool 1 with the ambient air, and is used for discharging the gas in the receiving pool 1, so that the human body fluid can fill the receiving pool 1 and tightly adhere to the diffusion membrane 5. Meanwhile, the screw hole 403 can be used as a sampling port for extracting the human body fluid in the receiving pool 1.

[0036] The diffusion membrane 5 is laid in the groove 401 of the linking cover 4, and is fixed by screwing the linking cover 4 and the diffusion pool 8. The upper and lower surfaces of the diffusion membrane 5 are respectively tightly adhered to the sample in the diffusion pool 8 and the human body fluid in the receiving pool 1. The diffusion membrane 5 is made of dialysis membrane or skin membrane. The diffusion membrane 5 makes the sample contact the human body fluid only through the membrane surface, and realizes the one-way migration of the chemical substances in the sample to the human body fluid. In addition, the position of the diffusion membrane 5 can be fixed by screwing the linking cover 4 and the diffusion pool 8. Meanwhile, the combination of the receiving pool 1 and the linking cover 4 can be disassembled by only screwing, which is convenient for replacing the adsorption phase 2 in the receiving pool 1.

[0037] The small sealing ring 6 can be stacked on the diffusion membrane 5, and the diffusion pool 8 is tightly pressed against the small sealing ring 6. The large sealing ring 3 can be nested in the ring groove 402 of the linking cover 4. The small sealing ring 6 and the large sealing ring 3 can be used to enhance the sealing performance of the device, prevent liquid leakage at the linking cover 4 and the diffusion pool 8, and prevent liquid leakage at the receiving pool 1.

[0038] The adsorption phase 2 can be placed in the receiving pool 1, and is made of XAD resin, polydimethylsiloxane or polyethylene. The adsorption phase 2 has an enrichment effect on the target chemical substances, and can improve the detection amount of the target chemical substances.

[0039] The sealing member is used to block the screw hole 403, and is preferably a screw 7. The screw 7 can cut off the communication between the receiving pool 1 and the atmosphere outside the device, ensure the sealing performance of the device, resist the environmental background interference, and prevent the human body fluid from being reduced due to evaporation.

[0040] The top cover 9 covers the top of the diffusion pool 8. After the device is assembled, the whole device is in a closed form, which can prevent the human body fluid in the receiving pool 1 from being reduced due to liquid leakage or evaporation, and can resist the environmental background interference.

[0041] Preferably, the receiving pool 1, the linking cover 4, the screw 7, the diffusion pool 8 and the top cover 9 are made of stainless steel, glass or plastic.

[0042] When the sampler is used, the following steps are performed:

[0043] (1) Assemble the transdermal migration device, including the receiving pool 1, the adsorption phase 2, the large sealing ring 3, the linking cover 4, the diffusion membrane 5, the small sealing ring 6, the screw 7, the diffusion pool 8 and the top cover 9. The receiving pool 1 is used to contain the human body fluid, the linking cover 4 is used to link the receiving pool 1 and the diffusion pool 8, and the diffusion pool 8 is used to place the sample.

[0044] (2) Fill the receiving pool 1 with human body fluid, and plug the screw hole 403 with the screw 7. Place the sample in the diffusion pool 8 and lay it on the diffusion membrane 5.

[0045] (3) Place the device in a 37 o C incubator to conduct the transdermal migration test.

[0046] (4) Replace the adsorbent and human body fluid regularly, and treat the adsorbent and human body fluid respectively and detect and analyze them on a gas chromatography-mass spectrometer and other instruments.

[0047] The above examples are the preferred embodiments of the present application, but the embodiments of the present application are not limited to the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application should be equivalent replacement methods, and all are included in the protection scope of the present application.

Claims

1. A transdermal migration device, characterized in that: It includes a receiving cell for holding human body fluids, an adsorption phase, a connecting cover, a diffusion membrane, a sealing element, a diffusion cell for placing samples, and a top cover; The adsorbent phase is placed in the receiving cell; The connecting cover is grooved with a hollow center, and its upper and lower ends are detachably connected to the diffusion pool and the receiving pool, respectively. The diffusion membrane is placed flat in the groove of the connecting cover and fixed by the diffusion cell. The upper and lower surfaces of the diffusion membrane are in close contact with the sample in the diffusion cell and the human body fluid in the receiving cell, respectively. The connecting cover has a vent hole for connecting the receiving pool with ambient air, and the seal is removably plugged into the vent hole; The top cover covers the top of the diffusion pool; The connecting caps are threaded to the diffusion cell and the receiving cell respectively, and the diffusion membrane is fixed by screwing the connecting caps into the diffusion cell.

2. The transdermal migration device according to claim 1, characterized in that: The lower circumferential surface of the connecting cover is provided with an external thread, and the upper circumferential surface of the receiving pool is provided with a matching internal thread; the upper circumferential surface of the connecting cover is provided with an internal thread, and the lower circumferential surface of the diffusion pool is provided with a matching external thread.

3. The transdermal migration device according to claim 1, characterized in that: A small sealing ring is provided on the diffusion membrane, and the diffusion cell is pressed tightly against the small sealing ring.

4. The transdermal migration device according to claim 1, characterized in that: A large sealing ring is provided at the connection between the connecting cover and the receiving pool.

5. A transdermal migration device according to claim 4, characterized in that: The lower circumference of the connecting cover is provided with an annular groove, and the large sealing ring is fitted into the annular groove.

6. The transdermal migration device according to claim 1, characterized in that: The vent is a screw hole, and the seal is a screw, with the screw threaded into the screw hole.

7. The transdermal migration device according to claim 1, characterized in that: The adsorbent phase is made of XAD resin, polydimethylsiloxane or polyethylene.

8. A transdermal migration device according to claim 1, characterized in that: The diffusion membrane is made of dialysis membrane or skin membrane.

9. A transdermal migration device according to claim 1, characterized in that: The receiving pool, connecting cover, diffusion pool, and top cover are made of stainless steel, glass, or plastic.

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