A bactericidal composition containing a triazole compound and use thereof

Abstract

The present application relates to a kind of triazole-containing fungicidal composition and its application, comprising active ingredient A and active ingredient B, the active ingredient A is the compound shown in formula (I), active ingredient B is triazole compound, the triazole compound is selected from any one of chlorothalonil, fluquinconazole, tebuconazole, leaf fungicide, the mass ratio of active ingredient A and active ingredient B is 1:45~40:1.The fungicidal composition or its preparation of the present application can enhance efficacy, reduce drug dosage, while improving control effect and delaying the development of drug resistance.

Description

Technical Field

[0001] This invention relates to the field of pesticide fungicide technology, specifically to a fungicide composition containing triazole compounds and its application. Background Technology

[0002] Compound (I) is a quinoline carboxamide fungicide developed by Syngenta. It has a broad spectrum of control, is safe and highly effective, and can effectively control various fungal diseases. Its CAS Registry Number is 2132414-06-1 (racemic 2132414-04-9). Its Chinese chemical name is N-(2R)-[1,3-dimethyl-1-(phenylmethyl)butyl]-8-fluoro-3-quinoline carboxamide-N-(2S)-[1,3-dimethyl-1-(phenylmethyl)butyl]-8-fluoro-3-quinoline carboxamide. Its chemical structure is as follows:

[0003] (I).

[0004] Mefentrifluconazole is a broad-spectrum, highly effective fungicide with systemic properties, possessing both protective and therapeutic effects. It belongs to the class of sterol demethylation inhibitors. Its mechanism of action is to inhibit the biosynthesis of ergosterol, thereby inhibiting cell growth and ultimately leading to cell membrane collapse. Its CAS Registry Number is 1417782-03-6.

[0005] Metconazole is a novel broad-spectrum systemic fungicide that acts on C in the biosynthesis of sterols in membranes. 14 - Demethylase. It exhibits excellent activity against *Syngonium* and rust diseases, providing both protection and treatment; it can be used for stem and leaf treatment as well as seed treatment.

[0006] Tebuconazole is a new generation of highly effective broad-spectrum systemic fungicide with protective, curative, and eradicative effects. It has a broad fungicidal spectrum and a long-lasting effect, making it a highly effective fungicide for seed treatment or foliar spraying of important economic crops. It is widely applicable to the prevention and control of various fungal diseases in fruit trees, vegetables, rapeseed, peanuts, grapes, tea trees, lawns, ornamental plants, and various cereal crops.

[0007] Epoxiconazole is a systemic triazole fungicide that can be absorbed by the stems and leaves of plants and translocated upwards and outwards. It is effective against wheat rust, rice sheath blight, rice false smut, and banana leaf spot. It not only possesses excellent protective, curative, and eradicative activity but also exhibits systemic and relatively good residual activity.

[0008] Triazole fungicides utilize the coordination of the triazole ring with the iron atom of the iron porphyrin center within the fungus to inhibit the formation of iron-oxygen complexes in the iron porphyrin. These fungicides are primarily used to treat plant diseases caused by ascomycetes, deuteromycetes, and basidiomycetes. By inhibiting the biosynthesis of ergosterol within the fungus, they cause abnormal fungal membrane function and cell wall damage, thereby achieving fungal suppression and killing. Pesticides are an important means of preventing and controlling plant diseases and pests. This invention has conducted in-depth research on the application of compound (I) to plant diseases. Extensive experimental results show that compound (I) combined with triazole fungicides, especially with chlorfluazuron, flutriafol, tebuconazole, and tebuconazole, under certain mass ratios, can effectively improve the control effect and has a significant synergistic effect. Summary of the Invention

[0009] Based on the above, the present invention aims to provide a fungicide composition and its formulation containing triazole compounds, mainly used for the prevention and control of plant fungal diseases. The composition or its formulation can enhance efficacy, reduce dosage, prolong the duration of action, delay the development of resistance, and has high safety for crops.

[0010] This objective is achieved through the following technical solution: a bactericidal composition containing triazole compounds, comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: (I) Active ingredient B is a triazole compound, which is selected from any one of chlorfluazuron, flutriafol, tebuconazole, and tebuconazole;

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:45 to 40:1;

[0012] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:32 to 32:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20 to 25:11 or any value between the above values;

[0014] Furthermore, the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20, 1:17, 1:10, 1:5, 1:1, 3:1, 7:1, 10:1, 17:1, or 25:1.

[0015] Furthermore, the mass ratio of the compound of formula (I) to flutriafol is 1:30 to 30:11 or any value between the above values;

[0016] Furthermore, the mass ratio of the compound of formula (I) to flutriafol is 1:30, 1:13, 1:6, 4:3, 7:1, 22:1, or 30:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:32 to 32:11 or any value between the above values;

[0018] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:32, 1:20, 1:7, 3:2, 10:1, 16:1, or 32:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:27 to 27:11 or any value between the above values;

[0020] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:27, 1:16, 1:8, 7:2, 8:1, 16:1, or 27.

[0021] Furthermore, based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1-90 wt%.

[0022] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5-80 wt%.

[0023] Furthermore, the bactericidal composition, in addition to containing the active ingredient, also contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

[0024] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0025] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0026] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0027] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0028] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0029] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0030] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0031] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0032] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0033] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0034] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0035] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0036] Furthermore, the dosage form of the bactericidal composition is selected from solid dosage forms and / or liquid dosage forms and / or seed treatment formulations;

[0037] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0038] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0039] Furthermore, the powder is a free-flowing powdered preparation containing active ingredients suitable for spraying or spreading;

[0040] Furthermore, the granules are granular preparations containing active ingredients that are free-flowing and have a certain particle size range;

[0041] Furthermore, the spherical agent is a spherical preparation containing active ingredients (generally with a diameter greater than 6 mm);

[0042] Furthermore, the tablet is a tablet-shaped preparation containing active ingredients with a certain shape and size (usually having two planes or convex surfaces, with the distance between the two surfaces being less than the diameter).

[0043] Furthermore, the strip preparation is a strip or rod-shaped preparation containing active ingredients (generally a few centimeters long and a few millimeters wide / diameter, i.e., the length is greater than the diameter / width).

[0044] Furthermore, the wettable powder is a powdered preparation in which the active ingredient is dispersed in water to form a suspension;

[0045] Furthermore, the oil-dispersible powder is a powdered preparation in which the active ingredient is dispersed in an organic solvent to form a suspension;

[0046] Furthermore, the aforementioned milk powder is a powdered preparation in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert component, and dispersed in water to form an oil-in-water emulsion.

[0047] Furthermore, the water-dispersible granules are granular formulations that disintegrate in water and disperse the active ingredients into a suspension;

[0048] Furthermore, the emulsion granules are granular formulations in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert component, and dispersed in water to form an oil-in-water emulsion.

[0049] Furthermore, the water-dispersible tablets are tablet formulations that disintegrate in water and disperse the active ingredients into a suspension;

[0050] Furthermore, the soluble powder is a powdered preparation in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0051] Furthermore, the soluble granules are granular preparations in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0052] Furthermore, the soluble tablets are tablet-shaped preparations in which the active ingredient forms a true solution in water, and may contain inert components that are insoluble in water;

[0053] Furthermore, the soluble agent is a transparent or translucent liquid preparation containing active ingredients that is diluted with water, and may contain inert components that are insoluble in water;

[0054] Furthermore, the soluble agent is a gel-like preparation containing effective ingredients that is diluted with water to form a true solution;

[0055] Furthermore, the oil is a homogeneous liquid preparation containing active ingredients, diluted (or undiluted) with an organic solvent;

[0056] Furthermore, the spreading oil is an oil containing effective ingredients that automatically diffuses into an oil film on the water surface;

[0057] Furthermore, the emulsifiable concentrate is a homogeneous liquid formulation containing active ingredients that is diluted and dispersed in water into an emulsion.

[0058] Furthermore, the latex is a latex preparation containing effective ingredients that is diluted and dispersed in water into an emulsion.

[0059] Furthermore, the dispersible liquid is a homogeneous liquid formulation containing active ingredients that is diluted and dispersed in water to form a suspension;

[0060] Furthermore, the ointment is a water-based ointment preparation containing effective ingredients that can form a film, and is generally used directly;

[0061] Furthermore, the water-in-water emulsion is a formulation in which the active ingredient (or its organic solution) forms an emulsion liquid in water;

[0062] Furthermore, the oil emulsion is an active ingredient (or its aqueous solution) forming an emulsion liquid formulation in oil;

[0063] Furthermore, the microemulsion is a microemulsion liquid formulation in which the active ingredient is transparent or translucent in water, and can be used directly or after dilution with water;

[0064] Furthermore, the liposome is an oil or fat-based viscous preparation containing active ingredients, which is generally used directly;

[0065] Furthermore, the suspending agent is a stable suspension of active ingredients dispersed in water as solid particles, and is generally diluted with water before use;

[0066] Furthermore, the microcapsule suspension is a stable suspension formulation in which microcapsules containing active ingredients are dispersed in a liquid.

[0067] Furthermore, the oil suspension is a stable suspension formulation in which the active ingredient is dispersed in a liquid as solid particles, and is generally diluted with an organic solvent before use;

[0068] Furthermore, the dispersible oil suspension is a stable suspension formulation in which the active ingredient is dispersed as solid particles in a non-aqueous medium, and is generally diluted with water before use;

[0069] Furthermore, the suspension emulsion is a heterogeneous liquid formulation in which the active ingredients are stably dispersed in a continuous aqueous phase in the form of solid microparticles and water-insoluble microdroplets.

[0070] Furthermore, the microcapsule suspension-suspension agent is a stable suspension liquid formulation in which the active ingredients are dispersed in water as microcapsules and solid particles;

[0071] Furthermore, the microcapsule suspension-water emulsion is a heterogeneous liquid formulation in which the active ingredient is stably dispersed in a continuous aqueous phase in the form of microcapsules and tiny droplets.

[0072] Furthermore, the microcapsule suspension-emulsion is an active ingredient that is stably dispersed in a continuous aqueous phase in the form of microcapsules, solid particles and tiny droplets to form a heterogeneous liquid formulation.

[0073] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable formulation, wherein the formulation is a microemulsion, water-in-oil emulsion, suspension, dispersible oil suspension, soluble concentrate, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water-dispersible granules, wettable powder, granules, seed treatment suspension, or seed treatment dry powder.

[0074] Furthermore, the formulation is a microemulsion, emulsifiable concentrate, suspension concentrate, water emulsion, water-dispersible granules, or seed treatment suspension.

[0075] The present invention also discloses the application of the bactericidal composition described above in the prevention and control of plant diseases;

[0076] Furthermore, the plant diseases mentioned are plant diseases caused by fungi or bacteria;

[0077] Furthermore, the plant diseases mentioned are plant diseases caused by fungi;

[0078] Furthermore, the plant diseases caused by the fungi mentioned are wheat leaf rust and wheat powdery mildew.

[0079] The beneficial effects of this invention are:

[0080] When the compound shown in formula (I) is combined with chlorfluazuron, flutriafol, tebuconazole, and tebuconazole in a certain proportion, its activity and fungicidal effect are not simply the sum of the activities of each component. It has a significant synergistic effect on wheat leaf rust and wheat powdery mildew, which can reduce the amount of each single agent used, reduce pesticide costs, and has good safety for crops, meeting the safety requirements of pesticide formulations, and has the effect of increasing yield and ensuring income. Detailed Implementation

[0081] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0082] Indoor bioassay for wheat leaf rust

[0083] Experimental basis: Refer to the Agricultural Industry Standard of the People's Republic of China, Indoor Bioassay Test Guidelines for Pesticides, Part 15: Control of Wheat Leaf Rust Test Pot Method NY / T 1156.15-2008.

[0084] 97% chlorfluazuron technical grade, 96% flutriafol technical grade, and 95% compound (I) technical grade are all provided by the Group's R&D Center.

[0085] Test crop: The wheat variety Luyuan 502, which is susceptible to leaf rust, was selected and potted. 20 seeds were sown in each pot. After emergence, 10 plants were selected and numbered when they grew to the 2-leaf and 1-heart stage.

[0086] Experimental target: Puccinia recondita Rob.ex Desm.f.sp.triticiErikss. et Henn., strain obtained from Shenyang Sinochem Pesticide & Chemical R&D Co., Ltd.

[0087] Preparation of spore suspension: Fresh urediniospores of rust fungus produced within 24 hours on diseased leaves were washed with a 0.1% Tween-80 aqueous solution and filtered through 2-4 layers of gauze to prepare a suspension with a concentration of 1×10⁻⁶. 5 A suspension of 1 spore / mL is prepared for later use.

[0088] Reagent preparation: Dissolve the test reagent in acetone, then dilute with 0.1% Tween 80 aqueous solution. Design different ratios according to the purpose of mixing and the activity of the reagent. Prepare the required series of mass concentrations of each group of single agents and each group of mixed agents according to the equal ratio method.

[0089] Chemical treatment: Spray the solution evenly onto the leaves until completely wet, and allow the solution to air dry naturally before use. A control group without the chemical treatment was included. Each concentration was repeated four times, with one plant per replicate.

[0090] Inoculation and culture: Inoculate with spore suspension by spraying. After inoculation, wheat seedlings are cultured in the dark at 20℃ for more than 12 hours with moist conditions. The optimal temperature for the moist conditions is 15~20℃. Then, they are cultured at 18~22℃ with a light ratio of L:D=12:12h.

[0091] Data survey: When the incidence rate in the blank control group reaches more than 80%, the incidence rate of each treatment will be investigated in a graded manner.

[0092] The grading method is as follows:

[0093] Level 0: No spore-bearing mass;

[0094] Grade 1: Spore accumulation accounts for less than 5% of the total leaf area;

[0095] Level 3: Spore masses occupy 5% to 10% of the entire leaf area;

[0096] Level 5: Spore masses occupy 10%~25% of the entire leaf area;

[0097] Level 7: Spore masses occupy 25%~50% of the entire leaf area;

[0098] Level 9: The spore mass accounts for more than 50% of the total leaf area.

[0099] Data statistics and analysis:

[0100] The disease index is calculated using the following formula.

[0101]

[0102] In the formula:

[0103] X—the disease index;

[0104] N i —the number of diseased leaves at each level;

[0105] i—the relative level value;

[0106] N—the total number of leaves investigated.

[0107] The control effect is calculated according to the following formula.

[0108]

[0109] In the formula:

[0110] P—the control effect, unit: %;

[0111] CK—the disease index of the blank control;

[0112] PT—the disease index of the medicament treatment.

[0113] Referring to the standard method of bioassay, the synergistic effect of the mixed medicaments is evaluated according to the co-toxicity coefficient method (CTC) of Sun Yunpei, that is, CTC≤80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC≥120 is synergistic effect.

[0114] The co-toxicity coefficient (CTC) is calculated according to the following formula.

[0115]

[0116] In the formula:

[0117] ATI—the actual toxicity index of the mixture;

[0118] S—the EC 50 of the standard medicament, unit: milligram per liter (mg / L);

[0119] M—the EC 50 of the mixture, unit: milligram per liter (mg / L).

[0120]

[0121] In the formula:

[0122] TTI—the theoretical toxicity index of the mixture;

[0123] TI A —the toxicity index of medicament A;

[0124] P A—The percentage content of drug A in the mixture, expressed as a percentage (%).

[0125] TI B —Toxicity index of Agent B;

[0126] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0127]

[0128] In the formula:

[0129] CTC – Cotoxicity Coefficient;

[0130] ATI – Actual Measured Toxicity Index of Mixtures;

[0131] TTI – Theoretical Toxicity Index of Mixtures.

[0132] The experimental results were calculated using DPS data processing software, and the toxicity regression equations and EC values ​​for single-dose and mixtures with different ratios of the test drug were derived. 50 and EC 90 And 95% confidence limit.

[0133] Wheat leaf rust

[0134] Indoor bioassay of compound (I) and chlorfluazuron for the control of wheat leaf rust

[0135] Table 1 shows that compound (I) controls wheat leaf rust EC. 50 The EC50 concentration of chlorfluazuron for controlling wheat leaf rust was 0.431 mg / L. 50 The concentration was 0.515 mg / L. Compound (I) mixed with chlorfluazuril at a mass ratio of 1:20 to 25:1 showed good efficacy, with a co-toxicity coefficient >120, indicating a synergistic effect. When the mass ratio was 1:1, the co-toxicity coefficient was 193.115, showing a significant synergistic effect.

[0136] Table 1. Results of the synergistic effect of compound (I) with chlorfluazuron in different ratios on wheat leaf rust.

[0137] Test reagents virulence regression equation Correlation coefficient r <![CDATA[EC 50 (95% confidence interval) / (mg / L) Cotoxicity coefficient (CTC) interaction Formula (Ⅰ) Compound A y = 1.179x + 5.431 0.997 0.431（0.396~0.470） / / Chlorothalonil B y = 1.185x + 5.342 0.999 0.515（0.494~0.537） / / A:B=1:20 y = 1.187x + 5.537 0.999 0.353（0.341~0.365） 144.551 Enhancement effect A:B=1:10 y = 1.211x + 5.593 0.999 0.324（0.311~0.337） 156.183 Enhancement effect A:B=1:5 y = 1.202x + 5.652 0.999 0.287（0.274~0.300） 173.797 Enhancement effect A:B=1:1 y = 1.166x + 5.716 0.999 0.243（0.237~0.249） 193.115 Enhancement effect A:B=3:1 y = 1.202x + 5.691 0.999 0.266（0.253~0.280） 168.918 Enhancement effect A:B=10:1 y = 1.217x + 5.700 0.998 0.266（0.246~0.287） 164.469 Enhancement effect A:B=25:1 y = 1.223x + 5.636 0.999 0.302（0.292~0.313） 143.616 Enhancement effect

[0138] Table 2 shows the EC50 of flutriafol for controlling wheat leaf rust. 50 The concentration was 0.434 mg / L. Compound (I) mixed with flutriafol at mass ratios of 1:45 to 30:1 showed good efficacy, exhibiting additive or synergistic effects. Specifically, at mass ratios of 1:30 to 30:1, the co-toxicity coefficient was >120, indicating a synergistic effect; at a mass ratio of 1:6, the co-toxicity coefficient was 202.123, demonstrating a significant synergistic effect.

[0139] Table 2. Results of the synergistic effect of compound (I) with flutriafol in different ratios on wheat leaf rust.

[0140] Test reagents virulence regression equation Correlation coefficient r <![CDATA[EC 50 (95% confidence interval) / (mg / L) Cotoxicity coefficient (CTC) interaction Formula (Ⅰ) Compound A y = 1.101x + 5.411 0.999 0.424（0.407~0.441） / / Fluticasone B y = 1.182x + 5.428 0.998 0.434（0.403~0.468） / / A:B=1:45 y = 1.149x + 5.499 0.999 0.368（0.346~0.392） 117.874 Additive effect A:B=1:30 y = 1.202x + 5.607 0.998 0.313（0.291~0.336） 138.553 Enhancement effect A:B=1:13 y = 1.201x + 5.613 0.999 0.309（0.296~0.322） 140.217 Enhancement effect A:B=1:6 y = 1.269x + 5.850 0.998 0.214（0.198~0.231） 202.123 Enhancement effect A:B=4:3 y = 1.203x + 5.792 0.997 0.219（0.202~0.238） 195.538 Enhancement effect A:B=7:1 y = 1.136x + 5.644 0.997 0.271（0.250~0.295） 156.909 Enhancement effect A:B=22:1 y = 1.185x + 5.594 0.998 0.316（0.292~0.341） 134.312 Enhancement effect A:B=30:1 y = 1.16x + 5.560 0.998 0.329（0.306~0.355） 128.971 Enhancement effect

[0141] Indoor bioassay for wheat powdery mildew

[0142] Test basis: NY / T1156.4-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 4: Pot Test for Control of Wheat Powdery Mildew"; NY / T 1156.6-2006 "Agricultural Industry Standard of the People's Republic of China - Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 6: Determination of Combined Effects of Mixtures".

[0143] Experimental target: wheat powdery mildew fungus (Erysiphe graminis), strain obtained from Shenyang Sinochem Pesticide & Chemical R&D Co., Ltd.

[0144] Test crop: Selected wheat (Liaochun 10) susceptible to powdery mildew in pots. After the seedlings grow to the 2-3 leaf stage, they are numbered for future use.

[0145] The technical grade of 97% chlorfluazuron, 95% tebuconazole, 95% tebuconazole, and 95% compound (I) were all provided by the Group's R&D Center.

[0146] Drug preparation: Dissolve the original drug in acetone first, then dilute it with 0.1% Tween 80 aqueous solution to prepare a single-dose stock solution. Design 5 groups of ratios according to the purpose of mixing and drug activity. Prepare 5 series of mass concentrations for each single agent and each group of ratios according to the equal ratio method.

[0147] Chemical treatment: The pesticide was evenly sprayed onto the prepared wheat seedlings using a spraying method and allowed to air dry naturally. A control group without pesticide was included. Each concentration treatment was replicated 4 times, with 3 pots per replicate and 10 seedlings per pot.

[0148] Inoculation and culture: Fresh powdery mildew spores produced on diseased wheat leaves within 24 hours were evenly shaken off and inoculated onto treated 2-leaf stage potted wheat seedlings. Then, they were placed in suitable conditions for culture.

[0149] Data survey: A tiered survey of incidence rates was conducted based on the blank control group. The following tiering method was used:

[0150] Grade 0: No lesions;

[0151] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0152] Grade 3: Lesions cover 6-15% of the entire leaf area;

[0153] Level 5: The lesion area accounts for 16 - 25% of the whole leaf area;

[0154] Level 7: The lesion area accounts for 26 - 50% of the whole leaf area;

[0155] Level 9: The lesion area accounts for more than 50% of the whole leaf area.

[0156] Data statistics and analysis:

[0157] The disease index is calculated according to the following formula

[0158]

[0159] In the formula:

[0160] X - disease index;

[0161] N i — Number of diseased leaves at each level;

[0162] i - relative value of the level;

[0163] N - total number of leaves surveyed.

[0164] The control effect is calculated according to the following formula

[0165]

[0166] In the formula:

[0167] P - control effect, unit is %;

[0168] CK - disease index of the blank control;

[0169] PT - disease index of the medicament treatment.

[0170] According to Sun Yunpei's co-toxicity coefficient method (CTC) to evaluate the synergistic effect of the mixed medicaments, that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect.

[0171] The co-toxicity coefficient (CTC) is calculated according to the following formula.

[0172]

[0173] In the formula:

[0174] ATI - measured toxicity index of the mixture;

[0175] S - EC of the standard medicament 50 , unit is milligram per liter (mg / L);

[0176] M - EC of the mixture 50 , unit is milligram per liter (mg / L).

[0177]

[0178] In the formula:

[0179] TTI – Theoretical toxicity index of the mixture;

[0180] TI A —The toxicity index of drug A;

[0181] P A —Percentage content of agent A in the mixture, expressed as percentage (%);

[0182] TI B —Toxicity index of Agent B;

[0183] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0184]

[0185] In the formula:

[0186] CTC – Cotoxicity Coefficient;

[0187] ATI – Actual Measured Toxicity Index of Mixtures;

[0188] TTI – Theoretical Toxicity Index of Mixtures.

[0189] The test results are shown in the table below:

[0190] wheat powdery mildew

[0191] Table 3 shows that compound (I) controls wheat powdery mildew EC. 50 The EC50 concentration of tebuconazole for controlling wheat powdery mildew is 1.135 mg / L. 50 The concentration was 0.696 mg / L. Compound (I) mixed with tebuconazole at mass ratios of 1:32 to 40:1 showed good efficacy, exhibiting additive or synergistic effects. Specifically, at mass ratios of 1:32 to 32:1, the co-toxicity coefficient was >120, indicating a synergistic effect; at a mass ratio of 10:1, the co-toxicity coefficient was 189.655, demonstrating a significant synergistic effect.

[0192] Table 3. Results of the synergistic effect of compound (I) with tebuconazole in different ratios on wheat powdery mildew.

[0193] Test reagents virulence regression equation Correlation coefficient r <![CDATA[EC 50 (95% confidence interval) / (mg / L) Cotoxicity coefficient (CTC) interaction Formula (Ⅰ) Compound A y = 1.149x + 4.937 0.997 1.135（1.039~1.24） / / Tebuconazole B y = 1.109x + 5.174 0.994 0.696（0.612~0.792） / / A:B=1:32 y = 1.287x + 5.379 0.995 0.508（0.452~0.570） 138.633 Enhancement effect A:B=1:20 y = 1.221x + 5.406 0.999 0.466（0.446~0.486） 152.159 Enhancement effect A:B=1:7 y = 1.183x + 5.434 0.995 0.430（0.383~0.483） 170.084 Enhancement effect A:B=3:2 y = 1.083x + 5.339 0.993 0.486（0.425~0.557） 186.488 Enhancement effect A:B=10:1 y = 1.182x + 5.292 0.996 0.566（0.511~0.628） 189.655 Enhancement effect A:B=16:1 y = 1.270x + 5.210 0.995 0.684（0.613~0.762） 159.999 Enhancement effect A:B=32:1 y = 1.309x + 5.183 0.991 0.725（0.615~0.856） 153.616 Enhancement effect A:B=40:1 y = 1.278x + 5.003 0.997 0.995（0.918~1.078） 112.342 Additive effect

[0194] Table 4 shows the EC50 of tebuconazole for controlling wheat powdery mildew. 50The concentration was 0.611 mg / L. Compound (I) mixed with tebuconazole at a mass ratio of 1:27 to 27:1 showed good efficacy, with a co-toxicity coefficient >120, indicating a synergistic effect. When the mass ratio was 7:2, the co-toxicity coefficient was 197.516, indicating a significant synergistic effect.

[0195] Table 4. Results of the synergistic effect of compound (I) with different ratios of tebuconazole on wheat powdery mildew.

[0196] Test reagents virulence regression equation Correlation coefficient r <![CDATA[EC 50 (95% confidence interval) / (mg / L) Cotoxicity coefficient (CTC) interaction Formula (Ⅰ) Compound A y = 1.127x + 4.923 0.998 1.169（1.093~1.251） / / Leaf azole B y = 1.103x + 5.236 0.997 0.611（0.56~0.666） / / A:B=1:27 y = 1.285x + 5.389 0.994 0.498（0.442~0.561） 124.819 Enhancement effect A:B=1:16 y = 1.215x + 5.409 0.999 0.460（0.450~0.471） 136.663 Enhancement effect A:B=1:8 y = 1.169x + 5.425 0.995 0.433（0.388~0.483） 149.012 Enhancement effect A:B=7:2 y = 1.028x + 5.317 0.994 0.492（0.433~0.558） 197.516 Enhancement effect A:B=8:1 y = 1.140x + 5.299 0.994 0.547（0.482~0.620） 194.023 Enhancement effect A:B=16:1 y = 1.138x + 5.197 0.999 0.672（0.641~0.704） 165.090 Enhancement effect A:B=27:1 y = 1.192x + 5.094 0.998 0.834（0.769~0.905） 135.741 Enhancement effect

[0197] Table 5 shows the EC50 of chlorfluazuron for controlling wheat powdery mildew. 50 The concentration was 1.257 mg / L. Compound (I) mixed with chlorfluazuron at a mass ratio of 1:17 to 17:1 showed good efficacy, with a co-toxicity coefficient >120, indicating a synergistic effect. When the mass ratio was 1:1, the co-toxicity coefficient was 179.305, indicating a significant synergistic effect.

[0198] Table 5. Results of the synergistic effect of compound (I) with chlorfluazuron in different ratios on wheat powdery mildew.

[0199] Test reagents virulence regression equation Correlation coefficient r <![CDATA[EC 50 (95% confidence interval) / (mg / L) Cotoxicity coefficient (CTC) interaction Formula (Ⅰ) Compound A y = 1.119x + 4.921 0.998 1.177（1.099~1.261） / / Chlorothalonil B y = 1.112x + 4.890 0.999 1.257（1.186~1.333） / / A:B=1:17 y = 1.100x + 5.074 0.992 0.857（0.742~0.991） 146.123 Enhancement effect A:B=1:5 y = 1.147x + 5.114 0.997 0.796（0.734~0.864） 156.146 Enhancement effect A:B=1:1 y = 1.115x + 5.188 0.998 0.678（0.630~0.730） 179.305 Enhancement effect A:B=7:1 y = 1.134x + 5.163 0.999 0.719（0.678~0.761） 165.012 Enhancement effect A:B=17:1 y = 1.135x + 5.156 0.997 0.728（0.672~0.789） 162.249 Enhancement effect

[0200] Formulation Examples

[0201] Preparation Example 1:

[0202] 40% Formula (I) Compound·Tebuconazole Wettable Powder (36:4)

[0203] Formulation: 36% of compound (I), 4% of tebuconazole, 5% of sodium polycarboxylate, 5% of dispersant NNO, 5.5% of sodium alkyl polyoxyethylene ether sulfonate, 2.5% of sodium lignosulfonate, 0.5% of sodium dioctyl succinate sulfonate, 5% of bentonite, and the balance is made up with kaolin.

[0204] Preparation method: The active ingredients, other functional additives and fillers are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0205] Preparation Example 2:

[0206] 45% Formula (I) Compound·Iprodione Water Dispersible Granules (35:10)

[0207] Formulation: 35% of compound (I), 10% of tebuconazole, 3% sodium dodecyl sulfate, 5.5% of naphthalene sulfonate formaldehyde condensate, 1% of sodium fatty alcohol polyoxyethylene ether sulfate, 2% of lignosulfonate BX, 6.5% of sodium lignin sulfonate, 5% of attapulgite, and the remainder is made up with kaolin.

[0208] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet milling, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0209] Preparation Example 3:

[0210] 42% Formula (I) Compound·Fluorazole Water Dispersible Granules (6:36)

[0211] Formulation: 6% of compound (I), 36% of flutriafol, 4.5% sodium dodecyl sulfate, 5% of naphthalene sulfonate formaldehyde condensate, 1% of sodium fatty alcohol polyoxyethylene ether sulfate, 2% of BX sizing agent, 6.5% of sodium lignosulfonate, 5% of diatomaceous earth, and kaolin to make up the balance.

[0212] Preparation method: Same as in preparation example 2.

[0213] Preparation Example 4:

[0214] 24% Formula (I) Compound·Clonopyram Suspension (12:12)

[0215] Formulation: 12% of compound (I), 12% of chlorfluazuron, 2.5% of alkylphenol polyoxyethylene ether, 3% of phenethylphenol polyether phosphate salt, 3% of sodium dioctyl succinate sulfonate, 1.5% of sodium lignosulfonate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of organosilicon defoamer, and deionized water to make up the balance;

[0216] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension.

[0217] Preparation Example 5:

[0218] 18% Formula (I) Compound·Iprodione Suspension (14:4)

[0219] Formulation: 14% of compound (I), 4% of tebuconazole, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of sodium dioctyl succinate sulfonate, 2.5% of naphthalene sulfonate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.45% of organosilicon defoamer, and deionized water to make up the balance;

[0220] Preparation method: Same as in preparation example 4.

[0221] Preparation Example 6:

[0222] 25% Formula (I) Compound·Tebuconazole Suspension (20:5)

[0223] Formulation: 20% of compound (I), 5% tebuconazole, 1% castor oil polyoxyethylene ether, 2.5% glycerol fatty acid ester polyoxyethylene ether, 3.5% phenethylphenol polyether phosphate salt, 3% sodium dioctyl succinate sulfonate, 1.5% calcium dodecylbenzene sulfonate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.02% benzisothiazolinone, 0.5% organosilicon defoamer, and deionized water to make up the balance;

[0224] Preparation method: Same as in preparation example 4.

[0225] Preparation Example 7:

[0226] 20% Formula (I) Compound·Fluorotriazole Suspension (3:17)

[0227] Formulation: 3% of compound (I), 17% of flutriafol, 1.5% of castor oil polyoxyethylene ether, 3% of phenethylphenol polyether phosphate salt, 3% of sodium dioctyl succinate sulfonate, 1.5% of calcium dodecylbenzene sulfonate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.45% of organosilicon defoamer, and deionized water to make up the balance;

[0228] Preparation method: Same as in preparation example 4.

[0229] Preparation Example 8:

[0230] 10% Formula (I) compound·tebuconazole emulsifiable concentrate (8.5:1.5)

[0231] Preparation formula: 8.5% of compound (Ⅰ), 1.5% of tebuconazole, 1.2% of BHT, 10% of propylene carbonate, 16% of DMF, 5.2% of cyclohexanone, 3% of calcium dodecylbenzenesulfonate, 12% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0232] Preparation method: Add the active ingredients to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto. Stir and mix evenly in a stirring mixing tank to obtain emulsifiable oil.

[0233] Preparation Example 9:

[0234] 10% Formula (I) compound·Imidacloprid EC (7.5:2.5)

[0235] Preparation formula: 7.5% of compound (Ⅰ), 2.5% of tebuconazole, 1.2% of BHT, 10% of propylene carbonate, 18% of DMF, 4.5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 12% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance.

[0236] Preparation method: Same as in preparation example 8.

[0237] Field efficacy trials

[0238] Field efficacy trial of wheat leaf rust

[0239] Experimental basis: Refer to "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Cereal Rusts (Leaf Rust, Stripe Rust, Stem Rust) with Fungicides (GB / T 17980.23-2000)";

[0240] Experiment location: Wheat field in Nanfan Village, Shouguang City, Shandong Province;

[0241] Experimental target: Puccinia recondita f.sp.tritici., the fungus that causes wheat leaf rust.

[0242] Experimental crop and variety: Wheat (Tanmai 98);

[0243] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 The cells are randomly arranged.

[0244] Test reagents:

[0245] Table 6 Test Treatment

[0246] medicine <![CDATA[Dosage g a.i / hm 2 > 24% Formula (I) Compound·Clonopyram Suspension (12:12) 80 20% Formula (I) Compound·Fluorotriazole Suspension (3:17) 80 30% Formula (I) Compound Suspension 200 400 g / L chlorfenapyr suspension 100 12.5% ​​Fluticasone Suspension 100 Water comparison /

[0247] Application method: Use conventional spraying method to spray evenly on both sides of the leaves.

[0248] Survey and statistical methods: The survey was conducted 10 days after the last application of the pesticide. Five points were randomly selected from the diagonal of each plot, and 20 plants were surveyed at each point. The top 3 leaves of each plant were surveyed (including the flag leaf if present). The plants were graded according to the percentage of disease spots on each leaf covering the entire leaf area.

[0249] Grading method:

[0250] Level 0: No disease;

[0251] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0252] Grade 3: Lesions cover 6% to 25% of the total leaf area;

[0253] Level 5: Lesions cover 26% to 50% of the total leaf area;

[0254] Level 7: Lesions cover 51% to 75% of the total leaf area;

[0255] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0256] Methods for calculating drug efficacy

[0257] Calculate the disease index and prevention efficacy using the following formula.

[0258]

[0259] During the experiment, wheat growth was observed to be good in all treatment plots, and no herbicide damage was observed in any treatment.

[0260] The test results are shown in the table below:

[0261] Table 7. Results of field efficacy trials of various treatments for controlling wheat leaf rust.

[0262]

[0263] As shown in the table above, the field efficacy test results indicate that the compound shown in (I) and its mixture with chlorfluazuron and flutriafol showed good control effects against wheat leaf rust. Ten days after the last application, the control efficacy of 24% compound (I)·chlorfluazuron suspension (12:12) and 20% compound (I)·flutriafol suspension (3:17) against wheat leaf rust was 90.84% ​​and 91.73%, respectively, both showing good control effects.

[0264] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. No extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0265] Field efficacy trial of pesticides for wheat powdery mildew

[0266] Test basis: The test was conducted in accordance with GB / T17980.22-2000 "Guidelines for Field Efficacy Tests of Pesticides: Control of Powdery Mildew in Cereals by Fungicides".

[0267] Experimental location: Wheat field in Hongze District, Huai'an City, Jiangsu Province. The previous crop in the experimental field was rice. The soil fertility was moderate. The cultivation conditions of all experimental plots were consistent and in line with local scientific agricultural practices.

[0268] Experimental target: wheat powdery mildew fungus (Blumeria graminis f.sp. tritici).

[0269] Experimental crop: Wheat (Xinong 9718).

[0270] Experimental treatments: The experimental plots for the test reagent, control reagent, and blank control were arranged in a randomized block design, with guard rows between adjacent plots. Each plot had an area of ​​20 m². 2 Each treatment was repeated 4 times.

[0271] Table 8 Test Treatment

[0272] medicine <![CDATA[Dosage g a.i / hm 2 > 25% Formula (I) Compound·Tebuconazole Suspension (20:5) 80 18% Formula (I) Compound·Iprodione Suspension (14:4) 80 30% Formula (I) Compound Suspension 200 430 g / L Tebuconazole Suspension 75 50% tebuconazole water dispersible granules 75 Water comparison /

[0273] Application time and method: The first application was carried out at the early stage of wheat powdery mildew (April 10, 2023). After preparing the pesticide solution according to the experimental design, it was evenly sprayed using a 3WBD-16 backpack electric sprayer. Two sprays were carried out, with a water volume of 450 kg / hm² for each application. 2 The second dose will be administered 7 days later.

[0274] Survey methods: Disease index was surveyed before application of the pesticide, and the efficacy was assessed 7 days after the last application. Five fixed sampling points were taken along the diagonal of each plot, with each point measuring 0.25m. 2 For each plant, examine the flag leaf and the first leaf below the flag leaf.

[0275] The wheat powdery mildew grading method was used to conduct a grading survey, and the total number of leaves and the number of diseased leaves at each level were recorded.

[0276] Grade 0: No lesions;

[0277] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0278] Grade 3: Lesions cover 6% to 15% of the total leaf area;

[0279] Level 5: Lesions cover 16% to 25% of the total leaf area;

[0280] Level 7: Lesions cover 26% to 50% of the total leaf area;

[0281] Level 9: The lesion area accounts for more than 51% of the total leaf area.

[0282] The efficacy of the drug is calculated using the following formula:

[0283] Calculate the disease index and prevention efficacy using the following formula.

[0284]

[0285] The test results are shown in the table below:

[0286] Table 9. Results of field efficacy trials of various treatment mixtures for controlling wheat powdery mildew.

[0287]

[0288] As shown in the table above, the compound shown in (I) and its mixture with chlorfluazuron and flutriafol showed good control effects against wheat powdery mildew. Seven days after the last application, the control efficacy of 25% compound (I)·tebuconazole suspension (20:5) and 18% compound (I)·tebuconazole suspension (14:4) against wheat powdery mildew was 83.33% and 85.39%, respectively, both showing good control effects.

[0289] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. No extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0290] Through indoor toxicity testing and field trials, the compound shown in formula (I) of this invention, combined with chlorfluazuron, flutriafol, tebuconazole, and tebuconazole, exhibited excellent control effects against wheat leaf rust and wheat powdery mildew. The fungicidal composition or formulation obtained by this invention demonstrates significant efficacy, exhibiting high efficiency, broad spectrum, low residue, long-lasting effect, and strong systemic properties. Furthermore, no phytotoxicity was observed in the experiments, indicating that the enhanced synergistic effect of the obtained fungicidal composition or formulation reduces production and usage costs while ensuring crop safety.

[0291] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition containing triazole compounds, characterized in that: The bactericidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I). Chemical structural formula: (I) Active ingredient B is a triazole compound, wherein the triazole compound is chlorfluazuron, and the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20~25:

1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to chlorfluazuron is 1:20, 1:17, 1:10, 1:5, 1:1, 3:1, 7:1, 10:1, 17:1, or 25:

1.

3. The bactericidal composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%.

4. The bactericidal composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 5-80 wt%.

5. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredient, the bactericidal composition contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

6. The bactericidal composition according to claim 5, characterized in that, The bactericidal composition is prepared into a pesticide-acceptable formulation, wherein the formulation is a microemulsion, emulsifiable concentrate, suspension concentrate, water-in-oil emulsion, water-dispersible granules, or seed treatment suspension.

7. The application of the bactericidal composition according to any one of claims 1-6 in the prevention and control of plant diseases, characterized in that, The plant diseases mentioned are wheat leaf rust and wheat powdery mildew.

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