Recombinant three-type collagen cosmetic for sensitive skin care and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a recombinant three-type collagen cosmetic for sensitive skin care and a preparation method of the recombinant three-type collagen cosmetic. The invention relates to the technical field of cosmetics, in particular to a facial mask which is prepared from, by weight, 12-18 parts of recombinant three-type collagen, 4-7 parts of ceramide 3, 6-9 parts of squalane, 8-10 parts of centella asiatica extract, 3-5 parts of sodium hyaluronate, 1.5-2.5 parts of allantoin and 5-8 parts of beta-glucan, the recombinant three-type collagen serves as a core component, and key support is provided for reconstruction of skin barriers by quickly filling intercellular spaces and strengthening the basic structure of skin. Meanwhile, other compounds are matched to lock skin moisture, reinforce an intercellular lipid bilayer and make a skin barrier more compact, meanwhile, the centella asiatica extract is added, the skin stress response can be rapidly pacified, release of inflammatory mediators such as histamine in the skin is inhibited, uncomfortable symptoms such as skin redness, itching and stabbing pain are relieved, and the skin care product is suitable for being used for skin care. The skin is in a long-term sedative state.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a recombinant type III collagen cosmetic for sensitive skin care and a preparation method thereof. Background Art

[0002] With the deterioration of the environment, the accelerated pace of life, and improper skin care habits, the number of people with sensitive skin has been increasing year by year. Sensitive skin has a thin stratum corneum and a fragile skin barrier function. It has poor tolerance to external physical, chemical, and biological stimuli and is prone to a series of uncomfortable symptoms such as redness, itching, stinging, dryness, and desquamation, which not only affects the appearance, but also brings physical and psychological troubles to patients.

[0003] Although there are a wide variety of skin care products on the market, cosmetics for sensitive skin still have many shortcomings. In order to achieve immediate results, traditional cosmetics often add a large amount of irritating ingredients such as fragrances, preservatives, and alcohol, which can easily induce or aggravate sensitive skin problems. Some products that claim to be suitable for sensitive skin are not very effective in repairing the skin barrier and soothing skin inflammation. They only have a surface moisturizing effect and cannot fundamentally improve the inherent fragility of sensitive skin.

[0004] Collagen is a key substance for maintaining skin elasticity and firmness. Type III collagen is abundant in infant skin. As age increases, its synthesis in adults gradually decreases, and the loss of people with sensitive skin is accelerated. The emergence of recombinant type III collagen brings new hope. It overcomes the immunogenicity risk of traditional animal-derived collagen and is highly compatible with the amino acid sequence of human collagen. In theory, it can accurately act on the dermis of the skin and fill the collagen gap.

[0005] However, the existing cosmetics containing recombinant type III collagen are not scientifically formulated and fail to fully exert their advantages. The product effects are often greatly reduced due to poor synergy with other ingredients.

[0006] In view of the urgent need for sensitive skin care and the limitations of existing products, there is an urgent need for a recombinant type III collagen cosmetic for sensitive skin to fill the market gap and provide better quality and more efficient skin care options for people with sensitive skin. Summary of the invention

[0007] The object of the present invention is to provide a recombinant type III collagen cosmetic for sensitive skin care and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A recombinant type III collagen cosmetic for sensitive skin care and a preparation method thereof, comprising the following raw materials in parts by weight: 12-18 parts of recombinant type III collagen, 4-7 parts of ceramide 3, 6-9 parts of squalane, 8-10 parts of Centella asiatica extract, 3-5 parts of sodium hyaluronate, 1.5-2.5 parts of allantoin, 5-8 parts of β-glucan, 0.5-1.5 parts of β-nicotinamide mononucleotide, 0.5-1.5 parts of disodium pyrroloquinoline quinone, and 2-4 parts of maltobionic acid.

[0010] Preferably, the following raw materials are included in parts by weight: 15 parts of recombinant type III collagen, 3:5 parts of ceramide, 8 parts of squalane, 9 parts of Centella asiatica extract, 4 parts of sodium hyaluronate, 2 parts of allantoin, 6 parts of β-glucan, 0.5 parts of β-nicotinamide mononucleotide, 1.5 parts of disodium pyrroloquinoline quinone, and 2 parts of maltobionic acid.

[0011] A method for preparing recombinant type III collagen cosmetics for sensitive skin care, S1, raw material pretreatment

[0012] Place the recombinant type III collagen powder in a sterile dry environment, filter it with a sterilizing filter with a pore size of 0.22μm to remove trace impurities for later use; place the ceramide 3 raw material in a vacuum drying oven at a temperature of 40-50℃ for 2-3 hours to remove excess moisture for later use; select plant-synthesized squalane, first pass it through an activated carbon adsorption column with a flow rate controlled at 1-2mL / min to remove residual pigments and odor impurities for later use; take the Centella asiatica extract, dissolve and dilute it with an ethanol solution, Centrifuge at 8000-10000 r / min for 10-15 minutes in a high-speed centrifuge, take the supernatant and set aside; sprinkle sodium hyaluronate into deionized water, swell for 2-4 hours, prepare sodium hyaluronate gel and set aside; grind allantoin into fine powder, pass through 80-100 mesh sieve and set aside; soak and disperse β-glucan with phosphate buffer solution with pH value of 7.0-7.4, then place in an ultrasonic cleaner, ultrasonically treat for 15-20 minutes, and set the power at 200-300 W for set aside.

[0013] S2. Mixing and stirring

[0014] In a stainless steel reactor, first add the treated recombinant type III collagen, turn on the stirring paddle, stir at a low speed of 200-300r / min, so that the collagen is evenly dispersed at the bottom of the reactor, and then slowly add ceramide 3, squalane, and Centella asiatica extract supernatant in sequence. Each time a raw material is added, stir for 5-0 minutes, and then add the next one. At the same time, the temperature in the reactor is controlled at 30-40°C. Then add the sodium hyaluronate gel, increase the stirring speed to 400-500r / min, stir for 15-20 minutes, then add allantoin fine powder and dispersed β-glucan, and continue stirring for 20-30 minutes to form a mixture;

[0015] S3, emulsification

[0016] The mixture formed in S2 is added with a pre-prepared emulsifier, wherein the emulsifier is a non-ionic emulsifier and the amount is 1%-3% of the total raw material weight. The emulsifier is turned on and emulsified at a speed of 2500-3500 r / min for 10-15 minutes, and the temperature is maintained at 40-50° C. After the emulsification is completed, the emulsifier is stopped, the temperature of the material in the reactor is reduced to 30-35° C., the speed is maintained at 200-300 r / min, and β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, and maltobionic acid are slowly added in sequence, and stirring is continued for 10-15 minutes after each ingredient is added;

[0017] S4, aging and filling

[0018] Transfer the mixed materials into a sterile stainless steel storage tank, seal it, and age it at 25-30℃ for 24-48 hours. After aging, turn on the vacuum degassing device to remove the bubbles remaining in the material, and then use a filling machine to seal and package it.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention uses recombinant type III collagen as the core ingredient, which quickly fills the intercellular gaps and strengthens the basic structure of the skin, providing key support for the reconstruction of the skin barrier. At the same time, it is combined with other compounds to lock in skin moisture, strengthen the intercellular lipid bilayer, and make the skin barrier denser. At the same time, the addition of Centella asiatica extract can quickly soothe the skin's stress response, inhibit the release of inflammatory mediators such as histamine in the skin, relieve skin redness, itching, tingling and other discomfort symptoms, and keep the skin in a long-term calm state. At the same time, the present invention is compounded with other compounds to replenish and lock in moisture for the skin, keep the skin hydrated and plump, and improve the problem of rapid moisture loss caused by damaged barriers in sensitive skin;

[0021] The present invention cooperates with β-nicotinamide mononucleotide (NMN), disodium pyrroloquinoline quinone (PQQ) and maltobionic acid to exert skin care effects far exceeding those of a single ingredient. NMN enters cells and is converted into NAD+, which provides energy for cells, accelerates metabolism, and promotes the synthesis and renewal of recombinant type III collagen. At the same time, NAD+ participates in DNA repair, maintains its coding gene normal, and reduces damage from external stimuli; PQQ has strong antioxidant properties, removes free radicals to protect recombinant type III collagen, activates mitochondria to provide energy for its synthesis and cell repair, and can also reduce inflammation of sensitive skin; maltobionic acid, as a mild organic acid, removes aged keratin, stimulates cell renewal, creates an active cell environment for recombinant type III collagen, and enhances skin elasticity and firmness. Compared with existing products, the three ingredients in this formula cooperate to comprehensively improve the skin's self-repair and damage resistance from multiple aspects such as cell energy supply, anti-oxidation, anti-inflammatory, exfoliation and cell renewal, and effectively improve sensitive skin inflammation, damage and other problems, and avoid exfoliation irritation to sensitive skin. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] Embodiment 1:

[0024] A method for preparing recombinant type III collagen cosmetics for sensitive skin care, weighing 15 parts of recombinant type III collagen powder and placing them in a clean bench. First, spread a thin layer of powder and turn on the laminar air flow for 5 minutes to loosen it. Then connect a sterilizing filter with a pore size of 0.22μm, filter at a filtration pressure of 0.15MPa, and collect in a sterile plastic bottle sterilized with ethylene oxide. Take 5 parts of ceramide 3 raw materials and put them in a vacuum drying oven, set the starting temperature to 40°C, increase 1°C to 50°C every 10 minutes, vacuum degree -0.085MPa, and dry for 2.5 hours. Take out and cool in a nitrogen-filled glove box, and put it into a light-proof sterile bag.

[0025] Use plant-synthesized squalane and let it pass through an activated carbon adsorption column (activated carbon filling height 2 / 3) at a flow rate of 1.5mL / min. Collect the effluent and put it into a light-shielding glass container and refrigerate. Take 9 parts of Centella asiatica extract and add an appropriate amount of 55% ethanol solution. Stir with a magnetic stirrer at 350r / min for 30 minutes at 30°C. Then use a high-speed centrifuge to accelerate to 8000r / min for 12 minutes. Take the supernatant and sterilize it through a 0.22μm filter membrane and store it in a brown glass bottle. Preheat 35°C deionized water, sprinkle 4 parts of sodium hyaluronate powder into the water that is continuously stirred (200r / min) at a rate of 1g / min, swell for 3 hours to obtain a gel, and transfer it to a sterile plastic bucket. Put 2 parts of allantoin in an agate mortar, break it and grind it for 10 minutes to pass through a 90-mesh sieve, grind the unsieved part again, and put it into a sterile bottle.

[0026] β-glucan: Soak 6 parts of β-glucan with 2 times the weight of phosphate buffer, pH = 7.2, stir while adding, put it in an ultrasonic cleaner, ultrasonicate at 40kHz, 250W for 18 minutes (stop for 10 seconds every 3 minutes), and transfer to a sterile centrifuge tube.

[0027] First add the treated recombinant type III collagen into the stainless steel reactor and stir at 200 r / min for 5 minutes.

[0028] Use a pipette to add ceramide 3, squalane, and the supernatant of Centella asiatica extract in sequence, stirring at 300 r / min for 8 minutes after each addition. Pass 35°C water through the jacket to control the temperature.

[0029] Pour in the sodium hyaluronate gel, increase the speed to 450r / min and stir for 18 minutes, monitoring the viscosity during the process. Then add allantoin powder and β-glucan, continue stirring for 25 minutes, and take samples from different parts to check until the mixture is uniform.

[0030] Prepare 10% polyglycerol-6 distearate (2% by weight of total raw materials) solution 1 hour in advance and dissolve it in 50°C deionized water. After mixing evenly, add the emulsifier solution at a drop speed of 1mL / min, emulsify at 2500r / min for 5 minutes, then emulsify at 3000r / min for 10 minutes, control the temperature of the jacket at 45°C, and monitor the particle size to 100-200nm with a laser particle size analyzer. After emulsification, stop the emulsifier, reduce the temperature of the material in the reactor to 30°C, maintain the speed at 200r / min, and slowly add β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, and maltobionic acid in turn, and continue stirring for 10 minutes after adding each component.

[0031] The material was transferred to a sterile stainless steel storage tank via a sterile pipeline and aged for 36 hours at 28°C and 40%-50% humidity, with vibration for 10 minutes every 6 hours (50 Hz, 1 mm amplitude).

[0032] After aging, vacuum degassing is performed at -0.09MPa for 30 minutes to remove the bubbles remaining in the material. The material is then sealed and packaged using a filling machine to obtain recombinant type III collagen cosmetics for sensitive skin care.

[0033] Embodiment 2:

[0034] Accurately weigh 12 parts of recombinant type III collagen powder, place it in a drying room that meets the Class 100 sterile standard, and filter it with a sterilizing filter with a pore size of 0.22μm to ensure that any fine impurities that may be mixed in are removed. The filtered collagen will be used in the next step. Prepare 4 parts of ceramide 3, put it in a vacuum drying oven with a set temperature of 40°C, set the drying time to 2 hours, thoroughly remove the excess water contained in the raw materials, and take it out after drying. Select plant-synthesized squalane, slowly introduce it into the activated carbon adsorption column, accurately control the flow rate to 1mL / min, and let the squalane flow through the column. The residual pigments and odor impurities are fully adsorbed by the activated carbon, and the treated squalane is collected for use. Take 8 parts of Centella asiatica extract, dissolve and dilute it with a 75% ethanol solution, transfer it to a high-speed centrifuge, set the speed to 8000r / min, centrifuge for 10 minutes, wait for the centrifuge to stop, carefully extract the supernatant and set it aside for use.

[0035] Evenly sprinkle 3 parts of sodium hyaluronate into sufficient deionized water and allow it to fully swell for 2 hours to finally obtain a sodium hyaluronate gel with appropriate viscosity.

[0036] Take 1.5 parts of allantoin, use a mortar and pestle to carefully grind it into fine powder, then pass it through an 80-mesh sieve, and collect the fine powder for later use.

[0037] β-glucan: Soak 5 parts of β-glucan with phosphate buffer with a pH value of 7.0, gently stir to disperse it, then put it into an ultrasonic cleaner, adjust the power to 200W, and ultrasonically treat it for 15 minutes. After treatment, take it out and set it aside.

[0038] In a clean and dry stainless steel reactor, first add the pretreated recombinant type III collagen, start the stirring paddle, set the speed to 200r / min for low-speed stirring, so that the collagen is evenly spread on the bottom of the reactor. In order, slowly add ceramide 3 and stir for 5 minutes; then add squalane and continue stirring for 5 minutes; then pour in the supernatant of Centella asiatica extract and stir for 5 minutes. During this process, the temperature of the reactor is stably maintained at 30°C. Then add sodium hyaluronate gel, increase the stirring speed to 400r / min, and continue stirring for 15 minutes. Finally, add allantoin fine powder and dispersed β-glucan, and then stir for 20 minutes to obtain a preliminary mixed material.

[0039] Add nonionic emulsifier cetearyl olive oil ester to the above mixture, the addition amount is 1% of the total raw material weight. Turn on the emulsifier, adjust the speed to 2500r / min, emulsify for 10 minutes, during the emulsification process, the temperature of the reactor is maintained at 40°C, so that the material forms a stable emulsified state, after the emulsification is completed, stop the emulsifier, reduce the temperature of the material in the reactor to 30°C, maintain the speed at 200r / min, and slowly add 0.5 parts of β-nicotinamide mononucleotide, 1.5 parts of pyrroloquinoline quinone disodium, and 2 parts of maltobionic acid in sequence, and continue stirring for 10 minutes after adding each ingredient.

[0040] The emulsified material is carefully transferred to a sterile stainless steel storage tank, tightly sealed, and placed in a temperature-controlled environment at 25°C for aging for 24 hours. After aging, the vacuum degassing equipment is turned on to remove the remaining bubbles in the material. Finally, the filling machine is operated to accurately fill the processed cosmetic raw materials into the pre-prepared packaging bottles, and the sealed packaging is completed. A recombinant type III collagen cosmetic for sensitive skin care is ready.

[0041] Embodiment three:

[0042] Take 18 parts of recombinant type III collagen powder, place it in a sterile drying room with an air purification system and humidity maintained below 30%, and filter it carefully with a 0.22μm sterilizing filter to ensure the purity of the powder. The filtered material is placed in a clean glass container for standby use. Take out 7 parts of ceramide 3 raw materials, put them in a vacuum drying oven with a temperature precisely controlled at 50°C, and dry them for 3 hours to allow excess water to fully evaporate. After drying, quickly transfer them to a dry sealed bag for storage to prevent moisture absorption. Select high-quality plant-synthesized squalane and let it flow through an adsorption column filled with sufficient activated carbon. The flow rate is stably controlled at 2mL / min. After adsorption and purification, collect them in a light-shielding glass bottle to reduce the potential impact of light on its quality. Weigh 10 parts of Centella asiatica extract, pour an appropriate amount of 95% ethanol solution to dissolve and dilute, put them in a high-speed centrifuge, set a high speed of 10000r / min, centrifuge for 15 minutes, and after centrifugation, quickly use a pipette to absorb the supernatant into a sterile centrifuge tube for standby use. Take 5 parts of sodium hyaluronate and gently sprinkle it into a large glass beaker filled with deionized water. Stir well and let it stand for 4 hours to swell. Stir occasionally during the period until a uniform, transparent sodium hyaluronate gel is formed. Grind 2.5 parts of allantoin into a very fine powder, sieve it through a 100-mesh sieve several times until the sieved material is fine powder, collect it in a clean plastic box, and cover it tightly to prevent dust from flying.

[0043] β-glucan: Soak 8 parts of β-glucan with phosphate buffer at pH 7.4. After it is initially dispersed, put it into an ultrasonic cleaner, set the power to 300W, and ultrasonically treat it for 20 minutes. The treated β-glucan solution is more uniform and stable.

[0044] In a pre-sterilized stainless steel reactor, first pour in the properly treated recombinant type III collagen, turn on the stirring paddle, maintain a speed of 300r / min, and allow the collagen to be evenly distributed at the bottom of the reactor. Carefully add ceramide 3, squalane, and Centella asiatica extract supernatant in turn. Stir for 10 minutes each time you add a raw material, and keep the temperature in the reactor at 40°C throughout the process. After adding sodium hyaluronate gel, increase the stirring speed to 500r / min and stir vigorously for 20 minutes to fully mix the gel with the previous raw materials. Finally, add allantoin fine powder and treated β-glucan, and then stir for 30 minutes to obtain a mixture with a uniform texture.

[0045] Add nonionic emulsifier, PEG-40 hydrogenated castor oil, to the mixture in an amount of 3% of the total raw material weight. Turn on the emulsifier, adjust the speed to 3500r / min, set the emulsification time to 15 minutes, and use the temperature control device to keep the temperature constant at 50°C during the emulsification process to form a delicate and stable emulsified system. After the emulsification is completed, stop the emulsifier, lower the temperature of the material in the reactor to 30°C, maintain the speed at 200r / min, and slowly add 1 part of β-nicotinamide mononucleotide, 1 part of disodium pyrroloquinoline quinone, and 2 parts of maltobionic acid in turn. After adding each ingredient, continue stirring for 10 minutes.

[0046] The emulsified material is slowly transferred to a sterile stainless steel storage tank, tightly sealed, and placed in a constant temperature room at 30°C for aging for 48 hours. After aging, the vacuum degassing device is turned on to remove the residual bubbles in the material, and then a high-precision filling machine is used to accurately fill the cosmetics into a light-proof, airtight packaging container to complete the sealed packaging process.

[0047] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0048] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A recombinant type III collagen cosmetic for sensitive skin care, characterized in that: The invention comprises the following raw materials in parts by weight: 12-18 parts of recombinant type III collagen, 4-7 parts of ceramide 3, 6-9 parts of squalane, 8-10 parts of Centella asiatica extract, 3-5 parts of sodium hyaluronate, 1.5-2.5 parts of allantoin, 5-8 parts of beta-glucan, 0.5-1.5 parts of beta-nicotinamide mononucleotide, 0.5-1.5 parts of disodium pyrroloquinoline quinone and 2-4 parts of maltobionic acid.

2. The recombinant type III collagen cosmetic for sensitive skin care according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15 parts of recombinant type III collagen, 3:5 parts of ceramide, 8 parts of squalane, 9 parts of Centella asiatica extract, 4 parts of sodium hyaluronate, 2 parts of allantoin, 6 parts of beta-glucan, 0.5 parts of beta-nicotinamide mononucleotide, 1.5 parts of disodium pyrroloquinoline quinone and 2 parts of maltobionic acid.

3. A method for preparing a recombinant type III collagen cosmetic for sensitive skin care according to any one of claims 1-2, characterized in that: The method comprises the following preparation steps: S1. Raw material pretreatment Place the recombinant type III collagen powder in a sterile dry environment, filter it with a sterilizing filter with a pore size of 0.22μm to remove trace impurities for later use; place the ceramide 3 raw material in a vacuum drying oven at a temperature of 40-50℃ for 2-3 hours to remove excess moisture for later use; select plant-synthesized squalane, first pass it through an activated carbon adsorption column with a flow rate controlled at 1-2mL / min to remove residual pigments and odor impurities for later use; take the Centella asiatica extract, dissolve and dilute it with an ethanol solution, Centrifuge at 8000-10000r / min for 10-15 minutes in a high-speed centrifuge, take the supernatant and set aside; sprinkle sodium hyaluronate into deionized water, swell for 2-4 hours, prepare sodium hyaluronate gel and set aside; grind allantoin into fine powder, pass through 80-100 mesh sieve and set aside; soak and disperse β-glucan with phosphate buffer solution with pH value of 7.0-7.4, then place in an ultrasonic cleaner, ultrasonically treat for 15-20 minutes, and set the power at 200-300W, and set aside; S2. Mixing and stirring In a stainless steel reactor, first add the treated recombinant type III collagen, turn on the stirring paddle, stir at a low speed of 200-300r / min, so that the collagen is evenly dispersed at the bottom of the reactor, and then slowly add ceramide 3, squalane, and Centella asiatica extract supernatant in sequence. Each time a raw material is added, stir for 5-0 minutes, and then add the next one. At the same time, the temperature in the reactor is controlled at 30-40°C. Then add the sodium hyaluronate gel, increase the stirring speed to 400-500r / min, stir for 15-20 minutes, then add allantoin fine powder and dispersed β-glucan, and continue stirring for 20-30 minutes to form a mixture; S3, emulsification The mixture formed in S2 is added with a pre-prepared emulsifier, wherein the emulsifier is a non-ionic emulsifier and the amount is 1%-3% of the total raw material weight. The emulsifier is turned on and emulsified at a speed of 2500-3500 r / min for 10-15 minutes, and the temperature is maintained at 40-50° C. After the emulsification is completed, the emulsifier is stopped, the temperature of the material in the reactor is reduced to 30-35° C., the speed is maintained at 200-300 r / min, and β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, and maltobionic acid are slowly added in sequence, and stirring is continued for 10-15 minutes after each ingredient is added; S4, aging and filling Transfer the mixed materials into a sterile stainless steel storage tank, seal it, and age it at 25-30℃ for 24-48 hours. After aging, turn on the vacuum degassing device to remove the bubbles remaining in the material, and then use a filling machine to seal and package it.