Cistanche deserticola cell wall breaking method and preparation method of cinnamon, monkshood and rehmannia root nourishing paste containing cell wall broken cistanche deserticola

Abstract

The invention relates to a cell wall breaking method of cistanche deserticola and a preparation method of cinnamon, monkshood and rehmannia root nourishing paste containing the cell wall broken cistanche deserticola. The cell wall breaking method of the cistanche comprises the following steps: S101, taking a cistanche raw medicinal material, removing floating beetles and wrinkles, cleaning the cistanche raw medicinal material with clear water, drying the cistanche raw medicinal material, forging the cistanche raw medicinal material, crushing the cistanche raw medicinal material into fine powder, and sieving the fine powder to obtain cistanche fine powder; s102, adding yellow rice wine into the cistanche fine powder, cooking, and stirring until the yellow rice wine is completely absorbed to obtain wine-processed cistanche fine powder; s103, purified water is added into the wine-processed cistanche fine powder, the mixture is put into an on-line high-shear emulsion homogenizer to be homogenized, and cistanche emulsion is obtained; and S104, enabling the cistanche emulsion and a carrier purified water to enter a high-pressure micro-jet nano homogenizer to carry out cell wall breaking, so as to obtain the nano-scale cell wall-broken cistanche. The bioactive components of the cistanche deserticola are promoted to be released to the maximum extent, the bioavailability of the cistanche deserticola is remarkably improved, and the cinnamon, monkshood and rehmannia root nourishing paste prepared from the cell-wall-broken cistanche deserticola can better play the effects of nourishing yin, tonifying yang, tonifying kidney and filling marrow.

Description

Technical Field

[0001] The present invention relates to the field of plant cell wall breaking, and discloses a method for breaking the cell wall of Cistanche deserticola and a method for preparing a Guifu Dihuang nourishing paste containing the cell wall-broken Cistanche deserticola. Background Art

[0002] Cistanche deserticola is a precious traditional Chinese medicine, known as the "desert ginseng". It ranks first in the prescriptions for tonifying the kidney and strengthening yang and increasing strength in the past dynasties. It has the effects of tonifying the kidney and strengthening yang, strengthening the liver and kidney, and benefiting the essence and qi. In 2020, Cistanche deserticola was approved by the National Health Commission as a substance for both medicinal and edible use. The aphrodisiac effect of Cistanche deserticola has been recorded in ancient medical books. The "Ben Jing" records that Cistanche deserticola "strengthens yin, benefits essence and qi, and cures seven injuries", which means that it tonifies the kidney and strengthens yang, and is used for kidney qi deficiency. Because Cistanche deserticola is different from other aphrodisiac drugs, it does not hurt yin while tonifying the kidney, and even if it is taken for a long time, it will not cause dry mouth. This has been recorded in the "Compendium of Materia Medica" that "it is not complete, so it has the title of calmness."

[0003] The main chemical components of Cistanche deserticola include phenylethanoid glycosides, iridoid terpenes, lignans, polysaccharides, alkaloids, etc. Among them, phenylethanoid glycosides include echinacoside and verbascoside, which are two biologically active ingredients in Cistanche deserticola. Echinacoside has antioxidant, anti-aging, neuroprotective, anti-inflammatory, and anti-tumor effects, while verbascoside has antioxidant, anti-tumor, memory improvement, and neuroprotective effects. In the conventional roasting methods in the past, the detection rate of these two very important bioactive ingredients was not high, which affected the efficacy of Cistanche deserticola and failed to fully exert the kidney-tonifying and aphrodisiac effects of Cistanche deserticola.

[0004] Ultrafine grinding technology is an advanced technology that emerged rapidly in the 1980s. This technology converts raw materials into micron or nanometer-level powders by mechanical or fluid dynamic means. This treatment method makes the powder have extremely fine particle size, large specific surface area, high surface activity, rapid chemical reaction and excellent physical properties. Due to its unique optical, electrical and magnetic properties, ultrafine grinding technology has been widely used in many fields such as metallurgy, food, medicine, cosmetics and aerospace in developed countries. Ultrafine grinding of traditional Chinese medicine refers to the micro-grinding of traditional Chinese medicine at the cell level, in which the central particle size should be kept below 75μm and the cell wall breaking rate should reach or exceed 90%. Doing so will not only not change the original pharmacodynamic material basis of traditional Chinese medicine, but also significantly improve the homogeneity of the powder and the bioavailability of the medicinal materials, thereby helping to improve the overall quality of the preparation. Mechanical ultrafine powder technology is also used in the processing of Cistanche deserticola, but the release rate of its two main bioactive ingredients is not ideal.

[0005] In recent years, a new high-energy homogenization technology called dynamic high-pressure microfluidization technology has emerged. It uses high pressure to send materials into an oscillating reactor, causing the materials to undergo a series of actions such as shearing, pulverization, oscillation, high-speed impact, and puffing, thereby achieving the purpose of material refinement, emulsification, homogenization, and modification. Dynamic high-pressure microfluidization can quickly prepare tiny emulsion droplets in a short period of time, overcoming the shortcomings of traditional homogenization methods such as long processing time or multiple sample processing. As a new homogenization technology, high-pressure microfluidization has now been widely used in the preparation of nanoemulsions to increase the release rate of nutrients and active factors.

[0006] Chinese medicine internal ointment is moist and delicate, and its efficacy is mainly tonic, and it also has a slow therapeutic effect, so it is also called Chinese medicine ointment, ointment, and commonly known as ointment prescription. Ointment has the advantages of high concentration of effective drugs, easy to carry, easy to store, and easy to use.

[0007] The name "Guifu Dihuang Pill" was first seen in the Shenqi Bawei Pill (or Cui's Bawei Pill) in Zhang Zhongjing's "Golden Chamber" in the Eastern Han Dynasty, which is mainly used to treat consumptive back pain. When Wu Qian (Qing Dynasty) wrote "Yi Zong Jin Jian·Shan Bu Ming Yi Fang Lun", the dried Rehmannia in Zhongjing's "Shenqi Bawei Pill" was changed to cooked Rehmannia, and the name was "Bawei Dihuang Pill" or Guifu Dihuang Pill, which is mainly used to treat insufficient kidney fire. The 1963 edition of the "Pharmacopoeia of the People's Republic of China" first included Guifu Dihuang Pill, which is composed of 8 ingredients: cooked Rehmannia, Chinese yam, cornus officinalis, Poria, peony bark, oriental water plantain, cinnamon, and processed aconite. It can be seen that this prescription has been passed down for thousands of years, and its function of warming and nourishing kidney yang has been recognized by doctors. Summary of the invention

[0008] In view of this, the present invention provides a method for breaking the cell wall of Cistanche deserticola and a method for preparing a Guifu Dihuang nourishing paste containing the cell-broken Cistanche deserticola. The present invention not only promotes the release of active ingredients in Cistanche deserticola, but also improves the bioavailability of Cistanche deserticola, and the preparation of Guifu Dihuang nourishing paste can better exert the nourishing effects of nourishing yin and strengthening yang, and tonifying the kidney and filling the marrow.

[0009] In a first aspect of the present disclosure, a method for disrupting the cell wall of Cistanche deserticola is provided, which comprises the following steps: S101 Take the original medicinal material of Cistanche deserticola, remove the floating shell and wrinkled skin, clean it with clean water, dry it, forge it, and then grind it into fine powder, and pass it through a 100-mesh sieve to obtain Cistanche deserticola fine powder; S102 Add 1-3 times (e.g., 1, 2, 3 times) the total weight of the medicinal material to the Cistanche deserticola powder, and add 8-12% yellow wine (e.g., 8, 10, 12% yellow wine) for steaming and stirring for 4-8 hours (e.g., 4, 5, 6, 7, 8 hours) until the yellow wine is completely absorbed, to obtain the wine-processed Cistanche deserticola powder; S103 The wine-processed Cistanche deserticola powder is added with 2-5 times (e.g., 2, 3, 4, 5 times) of purified water by weight, and then put into an online high shear homogenizer for homogenization at a speed of 1500-3500 rpm (e.g., 1500 rpm, 2000 rpm, 2500 rpm, 3000 rpm, 3500 rpm) to obtain a Cistanche deserticola emulsion; S104 The Cistanche deserticola emulsion and carrier pure water enter the high-pressure microfluidizer to break the cell wall (see Figure 1 , Figure 2 ), the pressure is selected to be 100-220 MPa (e.g. 100 MPa, 120 MPa, 140 MPa, 160 MPa, 180 MPa, 200 MPa, 220 MPa), and the flow rate is 50-150 mL / min (e.g. 50, 100, 150 mL / min) to obtain nano-scale cell-wall-broken Cistanche deserticola.

[0010] In a second aspect of the present disclosure, a method for preparing Guifu Dihuang nourishing paste is provided, which comprises the cell-wall-broken Cistanche deserticola described in the first aspect. The method comprises the following steps: S201: freeze-drying Radix Rehmanniae, Fructus Corni (processed), Rhizoma Dioscoreae, Poria, Lycium barbarum, and Fructus Schisandrae Chinensis, and then grinding them by an ultrafine grinder, passing them through 300-1800 mesh sieves (e.g., 300, 600, 900, 1200, 1500, 1800 mesh) respectively, and then mixing them to obtain mixed ultrafine powder; S202 Crushing peony bark, oriental water chestnut, cinnamon bark, aconite root (processed), epimedium, curculigo orchioides, and liquorice into pieces, adding 8-16 times (e.g., 8, 10, 12, 14, 16 times) of purified water in an amount equal to the total weight of the medicinal materials, heating to 100° C., boiling for 6-12 hours (e.g., 6h, 8h, 10h, 12h), filtering, and removing the residue to obtain a medicinal solution; S203 Add the liquid medicine described in S202, the mixed ultrafine powder described in S201, and the cell-wall-broken Cistanche deserticola described in S104, heat to 75-95°C (e.g. 75, 85, 95°C) in a special Chinese medicine paste-making machine, start the mixer, and then add deer antler glue, red date powder, and honey in turn, boil and concentrate to make a paste, perform quality inspection, and fill.

[0011] The disclosed Guifu Dihuang nourishing paste is a new medicine composition, which is prepared by combining the following medicinal materials in parts by weight: 40-80 parts of Cistanche deserticola, 140-180 parts of Rehmannia root, 60-100 parts of Cornus officinalis (processed), 60-100 parts of Chinese yam, 40-80 parts of Poria cocos, 10-40 parts of Lycium barbarum, 40-80 parts of Schisandra chinensis, 40-80 parts of Paeonia suffruticosa, 40-80 parts of Alisma orientalis, 10-40 parts of cinnamon bark, 10-40 parts of Aconite root (processed), 10-40 parts of Epimedium, 10-40 parts of Curculigo orchioides, 10-50 parts of Licorice, 5-30 parts of Deer Horn Glue, 40-80 parts of Red Date Powder and 60-100 parts of Honey.

[0012] In some optional embodiments of the present disclosure, the optimal combination of Guifu Dihuang tonic paste is calculated by weight: 60 parts of Cistanche, 160 parts of Rehmannia glutinosa, 80 parts of Cornus officinalis (processed), 80 parts of Dioscorea opposita, 60 parts of Poria, 20 parts of Lycium barbarum, 60 parts of Schisandra chinensis, 60 parts of Paeonia suffruticosa, 60 parts of Alisma orientalis, 20 parts of cinnamon, 20 parts of Aconite (processed), 20 parts of Epimedium, 20 parts of Curculigo orchioides, 30 parts of Licorice, 10 parts of Deer Horn Glue, 60 parts of Red Date Powder, and 80 parts of Honey.

[0013] In summary, the present disclosure has the following beneficial effects: The present invention not only improves the release rate of the main active ingredients of Cistanche deserticola, but also improves the bioavailability of Cistanche deserticola. By analyzing the release rate of the active ingredients of Cistanche deserticola before and after cell wall breaking, the release rates of the two main bioactive substances of Cistanche deserticola were increased by 60% and 40% respectively (see Table 1), and the bioavailability in the body was also improved accordingly.

[0014]

[0015] According to the second aspect of the present disclosure, the Guifu Dihuang nourishing paste is a brand-new drug combination carefully formulated on the basis of classical prescriptions, and the preparation process has been completely innovated, so its effects of nourishing yin, strengthening yang, tonifying the kidney and filling the marrow are greatly enhanced, and it has good palatability, which enhances the compliance of consumers (see Table 2).

[0016]

[0017] Attached photos: Figure 1 High-pressure microfluidizer for cell disruption Figure 2 Dynamic high-pressure microfluidizer plant cell wall disruption test DETAILED DESCRIPTION

[0018] The present disclosure is further described in detail below in conjunction with embodiments.

[0019] Prescription (weight ratio) 60 parts of Cistanche deserticola, 160 parts of Rehmannia glutinosa, 80 parts of Cornus officinalis (processed), 80 parts of Dioscorea opposita, 60 parts of Poria, 20 parts of Lycium barbarum, 60 parts of Schisandra chinensis, 60 parts of Paeonia suffruticosa, 60 parts of Alismatis, 20 parts of Cinnamon bark, 20 parts of Aconite (processed), 20 parts of Epimedium, 20 parts of Curculigo orchioides, 30 parts of Licorice, 10 parts of Deer Horn Glue, 60 parts of Red Date Powder, and 80 parts of Honey.

[0020] Preparation process The first step is to prepare cell wall-broken Cistanche deserticola, which comprises the following steps: (1) Take the raw material of Cistanche deserticola, remove the floating scales and wrinkled skin, wash it with clean water, dry it, crush it, and pass it through a 100-mesh sieve to obtain fine Cistanche deserticola powder; (2) using the Cistanche deserticola powder obtained in (1), adding 12-proof rice wine of 3 times the total amount of the medicinal material, steaming and stirring for 4 hours until the rice wine is completely absorbed, thereby obtaining the wine-processed Cistanche deserticola powder; (3) Add 3 times the weight of purified water to the fine powder of Cistanche deserticola obtained in (2) and put it into an online high shear homogenizer for homogenization at a speed of 3000 rpm to obtain a Cistanche deserticola emulsion; (4) The Cistanche deserticola emulsion obtained in (3) and purified water were used as carriers and placed in a high-pressure microfluidizer for cell wall disruption. The pressure was selected to be 220 MPa and the flow rate was 50 ml / min. Nano-scale cell wall-broken Cistanche deserticola D was obtained.

[0021] In the second step, 160 parts of Radix Rehmanniae Preparata, 80 parts of Cornus officinalis (processed), 80 parts of Dioscorea opposita, 60 parts of Poria cocos, 20 parts of Lycium barbarum and 60 parts of Schisandrae Chinensis are cleaned, freeze-dried and then crushed by an ultrafine grinder, passed through a 300-mesh sieve, and the powders are mixed to obtain ultrafine powder drug material A.

[0022] Step 3: Forge 60 parts of Paeonia suffruticosa, 60 parts of Rhizoma Alismatis, 20 parts of Cinnamon Bark, 20 parts of Aconite (processed), 20 parts of Epimedium, 20 parts of Curculigo orchioides, and 30 parts of Licorice into pieces and place them in a decoction pot, then add 12 times the total amount of the above medicinal materials in pure water, heat to 100°C and decoct for 6 hours. Filter and remove the residue to obtain liquid B.

[0023] The fourth step is to add the ultrafine powder A and the cell-broken Cistanche D to the liquid medicine B, put them into a professional Chinese medicine paste making machine, and start the machine to stir. The temperature does not exceed 85℃, and then add 80 parts of honey, 60 parts of red date powder, and 10 parts of antler glue in turn; when the liquid medicine is observed to be paste-like, stop heating, take samples for quality inspection, and if qualified, fill and get the finished product.

[0024] It is to be understood that the above embodiments are merely exemplary embodiments used to illustrate the principles of the present disclosure, but the present disclosure is not limited thereto. For those of ordinary skill in the art, various modifications and improvements can be made without departing from the spirit and substance of the present disclosure, and these modifications and improvements are also considered to be within the scope of protection of the present disclosure.

Claims

1. A method for breaking the cell wall of Cistanche deserticola and a method for preparing Guifu Dihuang nourishing paste containing the cell wall-broken Cistanche deserticola. The method comprises the following steps: The first aspect includes providing a method for breaking the cell wall of Cistanche deserticola, and the second aspect provides a method for preparing Guifu Dihuang nourishing paste, which includes the cell wall-broken Cistanche deserticola described in the first aspect.

2. The first aspect of claim 1 includes providing a method for disrupting the cell wall of Cistanche deserticola, characterized in that: The method comprises the following steps: S101 Take the original medicinal material of Cistanche deserticola, remove the floating shell and wrinkled skin, clean it with clean water, dry it, forge it, and then grind it into fine powder, and pass it through a 100-mesh sieve to obtain Cistanche deserticola fine powder; S102 Add 8-12% rice wine in an amount of 1-3 times the total weight of the medicinal materials to the Cistanche deserticola powder, steam and stir for 4-8 hours until the rice wine is completely absorbed, and obtain the wine-processed Cistanche deserticola powder; S103 Add 2-5 times the weight of purified water to the wine-processed Cistanche deserticola powder and put it into an online high shear homogenizer for homogenization at a speed of 1500-3500 rpm to obtain a Cistanche deserticola emulsion; S104 The Cistanche deserticola emulsion and carrier pure water are fed into a high-pressure microfluidizer for cell wall disruption. The pressure is 100-220 MPa and the flow rate is 50-150 mL / min to obtain nano-scale cell wall-broken Cistanche deserticola.

3. The second aspect of claim 1 provides a method for preparing Guifu Dihuang nourishing paste, which comprises the cell-wall-broken Cistanche deserticola described in the first aspect, and the method comprises its drug combination and preparation process.

4. The pharmaceutical combination according to claim 3, characterized in that The drug combination and weight proportion are as follows: 40-80 parts of Cistanche deserticola, 140-180 parts of Rehmannia root, 60-100 parts of Cornus officinalis (processed), 60-100 parts of Dioscorea opposita, 40-80 parts of Poria cocos, 10-40 parts of Lycium barbarum, 40-80 parts of Schisandra chinensis, 40-80 parts of Paeonia suffruticosa, 40-80 parts of Alisma orientalis, 10-40 parts of cinnamon bark, 10-40 parts of Aconite root (processed), 10-40 parts of Epimedium, 10-40 parts of Curculigo orchioides, 10-50 parts of Licorice, 5-30 parts of Deer Horn Glue, 40-80 parts of Red Date Powder and 60-100 parts of Honey.

5. The preparation process of the Guifu Dihuang nourishing paste according to claim 3, characterized in that: The preparation process comprises the following steps: S201 freeze-drying the medicinal materials of Radix Rehmanniae Preparata, Fructus Corni (processed), Rhizoma Dioscoreae, Poria, Lycium barbarum and Fructus Schisandrae Chinensis, and then grinding them by an ultrafine grinder, passing the powders through a 300-1800 mesh sieve, and mixing the powders to obtain mixed ultrafine powder; S202 Crushing peony bark, oriental water rhizome, cinnamon bark, aconite root (processed), epimedium, curculigo orchioides, and liquorice into pieces, adding purified water in an amount of 8-16 times the total amount of the medicinal materials, heating to 100° C., boiling for 6-12 hours, filtering, and removing the residue to obtain a medicinal solution; S203 Add the liquid medicine described in S202, the mixed ultrafine powder described in S201, and the cell-wall-broken Cistanche deserticola described in S104, heat to 75-95°C in a special Chinese medicine paste-making machine, start the mixer, and then add deer antler glue, red date powder, and honey in turn, decoct and concentrate into a paste, and then fill it after quality inspection.

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