Traditional Chinese medicine dry distillation preparation for treating livestock metritis and promoting pregnancy and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and discloses a traditional Chinese medicine dry distillation preparation for treating hysteritis of livestock and promoting pregnancy, which is prepared from the following raw materials in parts by weight: 10-50 parts of flatstem milkvetch seed, 10-50 parts of eucommia ulmoides, 10-50 parts of walnut leaf, 10-30 parts of hawthorn, 20-30 parts of pomegranate rind and 5-10 parts of dried ginger. The composition has few toxic and side effects, is free of accumulation, does not generate drug resistance, and realizes the effects of treating endometritis and promoting pregnancy.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese veterinary medicines, and relates to a traditional Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy and a preparation method thereof. Background Art

[0002] Livestock metritis is a common obstetric disease caused by improper midwifery, pathogenic microorganism infection and other reasons. It is also the main cause of female infertility in clinical practice, which has a serious impact on breeding production and causes huge economic losses to healthy breeding. Long-term use of antibiotics for treatment leads to bacterial resistance and drug residues. Especially for dairy cows, milk cannot be delivered to dairy factories, causing greater losses. Therefore, the use of Chinese medicine preparations to treat livestock metritis has become the preferred medication option. At present, veterinary pharmaceutical companies also have related therapeutic products, but the therapeutic effect is not very ideal. Summary of the invention

[0003] Aiming at the technical problem that the therapeutic effect of traditional Chinese veterinary medicine for treating livestock endometritis is not ideal, the present invention provides a traditional Chinese medicine dry distillation preparation for treating livestock endometritis and promoting pregnancy. The prescription has few toxic and side effects, is non-accumulative, does not produce drug resistance, and achieves the effects of treating endometritis and promoting pregnancy.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] The invention provides a traditional Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy. The preparation is prepared by dry distillation liquid of the following raw materials in parts by weight and adding auxiliary materials: 10-50 parts of Astragalus complanatus seeds, 10-50 parts of Eucommia ulmoides, 10-50 parts of walnut leaves, 10-30 parts of hawthorn, 20-30 parts of pomegranate peels and 5-10 parts of dried ginger.

[0006] Preferably, the auxiliary materials are thickeners, solubilizers and emulsifiers.

[0007] Preferably, the mass of the thickener is 1-10% of the mass of the dry distillation liquid, and the thickener is one of carbomer, methyl cellulose or sodium alginate.

[0008] Preferably, the mass of the solubilizer is 5-60% of the mass of the dry distillation liquid, and the solubilizer is one of ethanol or glycerol.

[0009] Preferably, the mass of the emulsifier is 1-60% of the mass of the dry distillation liquid, and the emulsifier is one of polyvinyl alcohol, Span 60 or Tween 80.

[0010] The present invention also provides a method for preparing the above-mentioned Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy, comprising the following steps:

[0011] 1) Weigh the raw materials according to the above weight proportions, crush the seeds of Astragalus membranaceus, Eucommia ulmoides, walnut leaves, pomegranate peel, hawthorn, and dried ginger into 80-100 meshes respectively, and then mix them evenly;

[0012] 2) ultrafinely grinding the mixture obtained in step 1) to 300-500 mesh;

[0013] 3) placing the ultrafine powder mixture obtained in step 2) into a microwave cracking furnace, setting the microwave frequency and extraction temperature, and collecting the condensed dry distillate;

[0014] 4) The dry distillation liquid obtained in step 3) is mixed with a thickener, a solubilizer and an emulsifier, and stirred uniformly, and the pH value is adjusted to 3.0-8.0 with an alkali solution to obtain a dry distillation preparation of traditional Chinese medicine.

[0015] Preferably, the microwave frequency is 915 MHz to 5.8 GHz, and the extraction temperature is 150 to 300°C, preferably 240 to 270°C, and more preferably 270°C.

[0016] In the prescription of the present invention:

[0017] (1) Astragalus membranaceus: sweet, warm. Enters the liver and kidney meridians. Function: warms and nourishes the liver and kidneys, consolidates semen and reduces urine. Indications: liver and kidney deficiency, waist and limb weakness, spermatorrhea, premature ejaculation, frequent urination. The mature seeds of Astragalus membranaceus, an annual herbaceous plant of the Leguminosae family, contain fatty oil, tannins, vitamin A substances, etc., which have the effects of contracting the uterus and preventing diuresis.

[0018] (2) Eucommia ulmoides: sweet and warm. Enters the liver and kidney meridians. Functions: nourishes the liver and kidneys, strengthens the tendons and bones, and stabilizes the fetus. Indications: low back pain due to kidney deficiency, weakness in the waist and limbs, rheumatic pain, and restless fetus.

[0019] (3) Walnut leaf: bitter, astringent, flat, toxic. Functions: detoxification, swelling. Used to treat elephantiasis, excessive leucorrhea, scabies.

[0020] (4) Hawthorn: sour, sweet, slightly warm. Enters the spleen, stomach, and liver meridians. Function: helps digestion and resolves stagnation, promotes qi and dissipates blood stasis. Mainly used to treat abdominal distension caused by indigestion, indigestion, and postpartum lochia.

[0021] (5) Pomegranate peel: sour, astringent, warm in nature, enters the large intestine meridian. Functions: astringent to the intestines, stops diarrhea, stops bleeding, and drives away parasites. Mainly used to treat diarrhea, blood in stool, rectal prolapse, and parasite accumulation.

[0022] (6) Dried ginger: spicy, slightly warm. Enters the lung, spleen, and stomach meridians. Efficacy: relieves cold, warms the middle and stops vomiting, resolves phlegm and stops cough. Indications: exogenous wind-cold, stomach cold vomiting, cold phlegm cough.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] According to the Chinese medicine prescription, the prescription of the present invention is as follows: the Astragalus membranaceus in the prescription can warm and nourish the liver and kidney, consolidate the essence and reduce urine, and the Eucommia ulmoides is warm in nature, nourishes the liver and kidney, and strengthens the tendons and bones, and the two are combined as the main medicine; the walnut leaf can detoxify and reduce swelling, kill bacteria and reduce inflammation, and is the ministerial medicine; the pomegranate peel can astringe the intestines and stop diarrhea, stop bleeding, and the hawthorn can excite the uterus and promote qi and dissipate blood stasis, and are all adjuvants; the dried ginger is slightly warm, relieves the exterior and dissipates cold, warms the middle and stops vomiting. The combination of the medicines can play the effects of nourishing the liver and kidney, clearing away heat and detoxifying, reducing inflammation and swelling, and promoting qi and dissipating blood stasis.

[0025] The present invention adopts a dry distillation extraction process. Compared with conventional water extraction and alcohol extraction, the clinical effect of the dry distillation extract is better than that of water extraction, alcohol extraction and powder. The six medicinal materials, including Astragalus, Eucommia, Walnut Leaf, Crataegus, Pomegranate Peel and Dried Ginger, are subjected to anaerobic pyrolysis during the dry distillation extraction process under negative pressure and high temperature conditions, and new substances such as guaiacol and furfural are generated, which not only retains the effects of the original drugs, but also enhances the effects between compound drugs. Furfural is the raw material of intermediates such as furan acrylic acid, furfurylamine fumaric acid, adipic acid and furfuryl alcohol, and is widely used in the synthesis of fine chemicals such as medicine, pesticides, veterinary drugs, dyes, spices, rubber additives and preservatives. Some derivatives of furfural have strong bactericidal ability and a wide antibacterial spectrum. Endometritis in dairy cows is mostly caused by infections such as bacteria and fungi. Therefore, in the investigation and extraction of dry distillation liquid, the furfural content is taken as the standard. In addition, the mature seeds of Astragalus membranaceus in the present invention contain fatty oil and tannins; Eucommia ulmoides contains pinoresinol diglucoside; the main effective ingredient of pomegranate peel is tannins; the active ingredients of walnut leaves are quinones, tannins, isoflavones, vitamins, hawthorn hawthorn pulp acid and hawthorn kernel, etc., and dried ginger contains gingerol, etc. The diversity of components can carry out cracking reaction under high temperature and anaerobic conditions, promote the cracking reaction, and increase the diversity of new cracking substances.

[0026] The present invention firstly grinds the medicinal material into coarse powder and then grinds it into ultrafine powder to obtain micron-grade powder material, the cell wall breaking rate of the medicinal material is greater than 95%, the surface area is increased, and the effective components are released faster, thereby increasing the extraction rate and extraction speed of subsequent dry distillation extraction. Adding alcohol is more conducive to dissolving the new substances therein, and then adding carbomer and polyvinyl alcohol 2000 makes the dry distillation liquid become a viscous gel-like liquid. During use, it is injected into the uterus and is not easy to be discharged, thereby reducing drug waste.

[0027] The prescription of the present invention has few toxic and side effects, no accumulation, no drug resistance, and achieves the effects of treating endometritis and promoting pregnancy. The present invention can replace antibiotics, has a relatively ideal treatment effect, and has a very broad application prospect. DETAILED DESCRIPTION

[0028] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means known to those skilled in the art. The test methods in the following examples are conventional methods unless otherwise specified.

[0029] The model of the pulverizer used in the following examples is: JYNU40-37CS; the model of the ultrafine pulverizer is: WFM-100; the model of the microwave cracking furnace is: LM-SD-15. The moisture content of the following medicinal materials is controlled within 8%. The ethanol concentration is 95%. Polyvinyl alcohol uses polyvinyl alcohol 2000, purchased from Jinan Ruihongde Chemical Technology Co., Ltd. Carbomer uses Carbomer 980, purchased from Shandong Tianqi Pharmaceutical Co., Ltd.

[0030] Example 1

[0031] The compound formula is as follows:

[0032] Compound formula 1: Astragalus membranaceus 500g, Eucommia ulmoides 500g;

[0033] Compound formula 2: Astragalus membranaceus 300g, Eucommia ulmoides 300g, and walnut leaf 400g;

[0034] Compound formula 3: Astragalus membranaceus 250g, Eucommia ulmoides 250g, Juglans regia leaves 250g, and Hawthorn 250g;

[0035] Compound formula 4: Astragalus membranaceus 200g, Eucommia ulmoides 200g, walnut leaf 200g, hawthorn 200g, pomegranate peel 200g;

[0036] Compound formula 5: Astragalus membranaceus 150g, Eucommia ulmoides 150g, walnut leaf 200g, hawthorn 200g, pomegranate peel 200g, dried ginger 100g;

[0037] Compound formula 6: Astragalus membranaceus 250g, Eucommia ulmoides 250g, walnut leaf 200g, hawthorn 200g, pomegranate peel 200g, dried ginger 100g;

[0038] Compound formula 7: Astragalus membranaceus 150g, Eucommia ulmoides 150g, walnut leaf 300g, hawthorn 300g, pomegranate peel 200g, dried ginger 100g;

[0039] Compound formula 8: Astragalus membranaceus 150g, Eucommia ulmoides 150g, walnut leaf 200g, hawthorn 200g, pomegranate peel 300g, and dried ginger 200g.

[0040] 1000g each of Astragalus membranaceus, Eucommia ulmoides, walnut leaf, hawthorn, pomegranate peel, and dried ginger, as well as compound formulas 1 to 8, were processed according to the following steps:

[0041] (1) According to the single drug and compound formula 1 to 8, grind them into coarse powder to 100 mesh using a pulverizer, and then mix them evenly in a mixer;

[0042] (2) crushing the medicinal material powder obtained in step (1) into 400 meshes using an ultrafine grinder;

[0043] (3) The ultrafine powder mixture obtained in step (2) is put into a microwave cracking furnace, the microwave frequency is set to 2.45 GHz, the cracking temperature is set to 260° C., and the condensed dry distillate is collected;

[0044] (4) 100 g of the dry distillate obtained in step (3) was mixed with 5 g of carbomer, 45 g of ethanol, and 40 g of Tween, and stirred to prepare a dry distillation perfusion solution, and the pH value was adjusted to 5.5 with 0.5 mol / L sodium hydroxide solution.

[0045] In addition, 3 portions of compound formula 6 were taken and coarsely ground into 100 meshes using a pulverizer, and then mixed evenly using a mixer, and then subjected to the following three treatments:

[0046] (1) The conventional alcohol extraction (5 times ethanol, 2 extractions, 1.5 h each time) and 1:2 times concentration (i.e. 1 mL is equivalent to 2 g of the original medicinal material) were performed to obtain the alcohol-extracted perfusion solution, which was recorded as the alcohol-extracted group.

[0047] (2) The extracts were extracted with water (8 times water, extracted twice, 1.5 h each time) and concentrated 1:2 times (i.e., 1 mL is equivalent to 2 g of the original medicinal material) to obtain the water-extracted perfusion solution, which was recorded as the water-extracted group.

[0048] (3) The samples were ultrafinely pulverized and then injected into the rumen via a gavage device, which was recorded as the ultrafine pulverization group.

[0049] The prepared test sample was tested for furfural content by high performance liquid chromatography, with furfural as the reference. The test results are shown in Table 1.

[0050] Table 1 Furfural detection results

[0051]

[0052]

[0053] It can be seen that among the single medicines, the furfural content of Astragalus membranaceus is the highest; compared with compound formulations 1 to 5, the content of compound formulation 5 is higher, which may be related to the diversity of Chinese medicine components, and compared with compound formulations 6 and 7, the furfural content of compound formulation 6 is higher, from which it can be inferred that Astragalus membranaceus and Eucommia ulmoides have a greater impact on the furfural content in the formulation; compared with compound formulation 5, the furfural content of samples obtained by dry distillation extraction process in the alcohol extraction group and water extraction group is higher, it can be inferred that furfural is more suitable for dry distillation extraction.

[0054] In order to obtain a more suitable dry distillation extraction temperature of the microwave cracking furnace, 500g of Astragalus membranaceus was used as the medicinal material, firstly coarsely ground to 100 mesh by a pulverizer, then ultrafinely ground to 400 mesh, and then dry distilled and extracted by a microwave cracking furnace, the microwave frequency was set to 2.45GHz, and the temperature in the microwave cracking furnace was set to 150℃, 180℃, 210℃, 240℃, 270℃, and 300℃ for extraction, and the condensed dry distillate was collected. The extraction rate and furfural content of the dry distillate were measured, as shown in Table 2.

[0055] Table 2 Different temperature distillation results

[0056]

[0057] Note: Extraction rate = volume of extracted distillate (ml) / weight of feed (g)*100%.

[0058] As shown in Table 2, with the increase of the dry distillation temperature, the extraction rate of the dry distillation liquid and the furfural content also increase accordingly. However, after the temperature exceeds 270°C, the extraction rate of the dry distillation liquid and the furfural content show a downward trend. Therefore, the optimal temperature range for dry distillation extraction is 240-270°C.

[0059] Example 2

[0060] In a certain ranch, there are 2600 lactating cows. From June to August 2024, the vaginal secretions of the second-born cows with metritis were scored. The scores are shown in the following table. The test products obtained by dry distillation extraction, alcohol extraction, water extraction and ultrafine grinding of the compound formula 6 in Example 1 were used for clinical trials, and compared with the rifaximin uterine injection and oxytetracycline uterine injection on the market. The comparative treatment results are shown in Table 3 below.

[0061] Table 3 Record and comparison of treatment results for uterine inflammation in cattle

[0062]

[0063] Notes: (1) Vaginal discharge is scored based on its color, odor, viscosity and amount of secretion. The higher the score, the more serious the condition. (2) Since the number of experimental animals is clinically ill, the onset time is not uniform and the number is uncontrollable, the number of animals in each experimental animal group is inconsistent. When statistically comparing, the comparison is based on the effectiveness rate and cure rate.

[0064] As shown in Table 2, the therapeutic effect of the dry distillation preparation of compound formula 6 on dairy cow metritis is better than similar products commonly used on the market, and the pregnancy rate after treatment is relatively high.

[0065] Example 3

[0066] In a sow breeding farm, there are 3,500 breeding sows. Vaginal secretions of sows with metritis were scored in sows with two or more parities within two months from July to August 2024. The test product obtained by dry distillation extraction of compound formula 6 was used for clinical trials and compared with rifaximin uterine injection and oxytetracycline uterine injection on the market. The comparative treatment results are shown in Table 4.

[0067] Table 4 Record and comparison of treatment results for sows with metritis

[0068]

[0069] Notes: (1) Vaginal discharge is scored based on its color, odor, viscosity and amount of secretion. The higher the score, the more serious the condition. (2) The number of experimental animals is clinically ill animals. The onset time is not uniform and the number is uncontrollable. Therefore, the number of animals in each experimental animal group is inconsistent. When statistically comparing, the comparison is based on the effectiveness rate and cure rate.

[0070] As shown in Table 4, the therapeutic effect of the compound formula 6 distillation preparation on sow metritis is better than similar products commonly used on the market, and the pregnancy rate after treatment is significantly higher than that of the control group of market products.

[0071] The embodiments described above are only preferred embodiments of the present invention and are only used to explain the present invention, not to limit the scope of implementation of the present invention. For those skilled in the art, other implementation methods can certainly be easily made by replacement or modification based on the technical contents disclosed in this specification. Therefore, all changes and improvements made on the principles of the present invention should be included in the scope of the patent application of the present invention.

Claims

1. A Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy, characterized in that: The method is prepared by adding auxiliary materials to the dry distillation liquid of the following raw materials in parts by weight: 10-50 parts of Astragalus complanatus seeds, 10-50 parts of Eucommia ulmoides, 10-50 parts of walnut leaves, 10-30 parts of hawthorn, 20-30 parts of pomegranate peels and 5-10 parts of dried ginger.

2. A Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy according to claim 1, characterized in that: The auxiliary materials are thickener, solubilizer and emulsifier.

3. A Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy according to claim 2, characterized in that: The mass of the thickener is 1-10% of the mass of the dry distillation liquid, and the thickener is one of carbomer, methyl cellulose or sodium alginate.

4. A Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy according to claim 2, characterized in that: The mass of the solubilizer is 5-60% of the mass of the dry distillation liquid, and the solubilizer is one of ethanol or glycerol.

5. A Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy according to claim 2, characterized in that: The mass of the emulsifier is 1-60% of the mass of the dry distillation liquid, and the emulsifier is one of polyvinyl alcohol, Span 60 or Tween 80.

6. The method for preparing the Chinese medicine dry distillation preparation for treating livestock uterine inflammation and promoting pregnancy according to any one of claims 1 to 5, characterized in that: The following steps are involved: 1) Weigh the raw materials according to the weight proportions of any one of claims 1 to 5, crush the seeds of Astragalus membranaceus, Eucommia ulmoides, walnut leaves, pomegranate peel, hawthorn, and dried ginger into 80-100 meshes respectively, and then mix them evenly; 2) ultrafinely grinding the mixture obtained in step 1) to 300-500 mesh; 3) placing the ultrafine powder mixture obtained in step 2) into a microwave cracking furnace, setting the microwave frequency and extraction temperature, and collecting the condensed dry distillate; 4) The dry distillation liquid obtained in step 3) is mixed with a thickener, a solubilizer and an emulsifier, and stirred uniformly, and the pH value is adjusted to 3.0-8.0 with an alkali solution to obtain a traditional Chinese medicine dry distillation preparation.

7. The method for preparing the Chinese medicine dry distillation preparation for treating uterine inflammation and promoting pregnancy in livestock according to claim 6, characterized in that: The microwave frequency is 915 MHz to 5.8 GHz, and the extraction temperature is 150 to 300° C.

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